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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean journal of applied entomology
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Journal DOI :
Korean Society of Applied Entomology
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Volume & Issues
Volume 45, Issue 3 - Dec 2006
Volume 45, Issue 2 - Aug 2006
Volume 45, Issue 1 - Apr 2006
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Biological Control of Thrips with Orius strigicollis (Poppius) (Hemiptera:Anthocoridae) and Amblyseius cucumeris (Oudemans) (Acari:Phytoseiidae) on Greenhouse Green pepper, Sweet pepper and Cucumber
Kim Jeong-Hwan ; Byeon Young-Woong ; Kim Yong-Heon ; Park Chang-Gyu ;
Korean journal of applied entomology, volume 45, issue 1, 2006, Pages 1~7
Effectiveness of Orius strigicollis (Poppius) and Amblyseius cucumeris (Oudemans) as natural enemies against thrips were evaluated on greenhouse green pepper, sweet pepper and cucumber respectively. Control efficacy was calculated by the formula,
is the average density of thrips on the plots in which any natural enemy was not released and
is the average density of thrips on the plots in which natural enemies were released respectively. As a result, control efficacies of O. strigicollis against Frankliniella occidentalis (Pergande) on green pepper and sweet pepper were
, respectively. In addition, control efficacy of it against Thrips palmi Kany on cucumber was 61.2-74.4%. Control efficacies of A. cucumeris against F. occidentalis on green pepper and sweet pepper were
, respectively. Control efficacy of it against T. palmi on cucumber was
. Field evaluation showed that the prompt applications of natural enemies were effective to reduce the density of thrips. In detail, to control F. occidentalis effectively on green pepper and sweet pepper in spring season, five to six individuals of O. strigicollis per crops should be released three to six times continuously. To control T. palmi effectively on cucumber in autumn, more than 100 individuals of A. cucumeris per crop should be released four times repeatedly.
Seasonal occurrence and damage by thrips on open red pepper in Jeonbuk Province
Moon Hyung-Cheol ; Cho In-Kwon ; Im Ju-Rock ; Goh Bok-Rae ; Kim Dae-Hyang ; Hwang Chang-Yeon ;
Korean journal of applied entomology, volume 45, issue 1, 2006, Pages 9~13
Seasonal occurrence of thrips and its damage on fruits were studied at in open field red pepper in Jeonbuk Province. The kind of thrips were Franklinella occidentalis and F. intonsa. The ratio of F. occidentalis was about 30% in periods of survey. Density of thrips increased in late May and showed peak occurrence in early to middle July. The peak occurrence of thrips was appeared at 4 pentad June, 2 pentad July, 3 pentad August, and 4 pentad September. The part of fruit damaged by thrips became discolored and roughed. When turned red, the colors of damaged parts changed from dark brown to yellowish brown. As a result, damage fruits by thrips decreased marketability. The percent of damaged fruits was highest in Imsil at 20.8% in early August. Density of thrips on flowers was highest in middle July.
Gene Structure of Cotesia plutellae Bracovirus (CpBV)-
and Its Expression Pattern in the Parasitized Diamondback Moth, Plutella xylostella
Kim Yong-Gyun ; Basio Neil A. ; Ibrahim Ahmed M.A. ; Bae Sung-Woo ;
Korean journal of applied entomology, volume 45, issue 1, 2006, Pages 15~24
Inhibitor kB (IkB)-like gene has been found in the genome of Cotesia plutellae bracovirus (CpBV), which is the obligatory symbiont of an endoparsitoid wasp, C. plutellae. The open reading frame of CpBV-IkB was 417 bp and encoded 138 amino acids. Four ankyrin repeat domains were found in CpBV-IkB, which shared high homology with other known polydnavirus IkBs. Considering a presumptive cellular IkB based on Drosophila Cactus, CpBV-IkB exhibited a truncated structure with deletion of signal-receiving domains, which suggested its irreversible inhibitory role in NFkB signal transduction pathway of the parasitized host in response to the wasp parasitization. CpBV-IkB was expressed only in the parasitized diamondback moth, Plutella flostella. Its expression was estimated by quantitative RT-PCR during parasitization period, showing a constitutive expression pattern from the first day of parasitization. An indirect functional analysis of CpBV-IkB was conducted and suggested a hypothesis of host antivirus inhibition
Sex Pheromone and Seasonal Occurrence of the peach leafminer, Lyonetia clerkella Linne
Yang Chang-Yeol ; Jeon Heung-Yong ; Kim Dae-Young ; Kim Hyeong-Hwan ;
Korean journal of applied entomology, volume 45, issue 1, 2006, Pages 25~30
The female sex pheromone of the peach leafminer, Lyonetia clerkella Linne (Lepidoptera: Lyonetiidae), was analyzed by coupled gas chromatographic-electroantennographic detector (GC-EAD). GC-EAD analyses of pheromone gland extract revealed a single compound that elicited responses from male antennae. Retention time on DB-1 column of EAD-active compound was identical to that of synthetic (145)-14-Methyl-1-octadecene (14Sme-1-18Hy). In field tests, sticky traps baited with synthetic 14Sme-1-18Hy alone were highly attractive to male. Traps with 0.1 mg dose showed the lowest catches, but there were no significant difference in the numbers of moth caught in traps baited with doses of 0.5 and 1.0 mg. The results of the field assays for longevity of pheromone traps showed that effectiveness of lures maintained for at least 8 weeks under field condition. The attractiveness of 14Sme-1-18Hy was not affected by the addition of butylated hydroxytoluene (BHT) in lures as an antioxidant. Traps baited with 0.5 mg 14Sme-1-18Hy were successfully used to monitor L. clerkella male flights. Analysis of seasonal trap catches over two years showed that moth flight activity in peach orchards occurred over a period of seven months with six generations in Suwon.
Inhibition of Nitric Oxide Production by ladybug extracts(Harmonia axyridis) in LPS-activated BV-2 cells
Han Sang-Mi ; Lee Sang-Han ; Yun Chi-Young ; Kang Seok-Woo ; Lee Kyung-Gill ; Kim Ik-Soo ; Yun Eun-Young ; Lee Pyeong-Jae ; Kim Sun-Yeou ; Hwang Jae-Sam ;
Korean journal of applied entomology, volume 45, issue 1, 2006, Pages 31~36
Inflammation in the brain has known to be associated with the development of a various neurologiacal diseases. The hallmark of neuro-inflammation is the activation of microglia, brain macrophage. Pro-inflammatory compounds including nitric oxide(NO) are the main cause of neuro-degenerative disease such as Alzheimer's disease. In the study, we examined whether Harmonia axyridis extracts inhibit the NO production by a direct method using Griess reagent, western blotting and by RT-PCR(Reverse Transcription-Polymerase Chain Reactionin) the gene expression of inducible nitric oxide synthase(iNOS). Distilled water
and methanol(MeOH) extracts of H. axyridis inhibited the protein expression of TNF-a(Tumor Necrosis Factor) and IL-6(Interleukin) in LPS (Lipopolysaccharide) stimulated BV-2 cells at the concentration of 100 ng/ml. Incubation of BV-2 cells with the extracts of
of MeOH inhibited the LPS induced NO and iNOS protein. And this inhibition of iNOS protein is concordant with the inhibition of iNOS mRNA expression. These data suggested that H. axyridis extracts may play a crucial role in inhibiting the NO production.
Attraction of the Garden Thrips, Frankliniella intonsa (Thysanoptera:Thripidae), to Colored Sticky Cards in a Nonsan Strawberry Greenhouse
Seo Mi-Ja ; Kim Sun-Jin ; Kang Eun-Jin ; Kang Myong-Ki ; Yu Yong-Man ; Nam Myeong-Hyeon ; Jeong Suk-Gee ; Youn Young-Nam ;
Korean journal of applied entomology, volume 45, issue 1, 2006, Pages 37~43
Catch of garden thrips, Frankliniella intonsa, on sticky traps was assessed by color and height in commercial plastic vinyl house strawberry crops grown on 40-cm-high trellises. Yellow, blue and white sticky cards were tested for color attractiveness, and upper (50 cm), lower (10 cm above the floor) and ground were for height. At each height, trapped thrips on yellow, blue and white were significantly different. Blue sticky cards were significantly greater than traps on yellow and white. The trapped thrips were also significantly greatest at 50 cm above the floor, less at lower and ground. However, there is no significantly different the population of garden thrips on the flower in each tested site.
Fumigant Toxicity of Pennyroyal and Spearmint oils against Western Flower Thrips, Frankliniella occidentalis
Han Jong-Been ; Ahn Ki-Su ; Lee Chong-Kyu ; Kim Gil-Hah ;
Korean journal of applied entomology, volume 45, issue 1, 2006, Pages 45~49
Fumigant toxicity of four plant essential oils (muguet flower, patchouli, pennyroyal, spearmint) were tested against the adults of western flower thrips, Frankliniella occidentalis. Among them, pennyroyal and spearmint oils showed 100% mortality at
air dose. Through the constituent analysis using GC and GC-MS, we confirmed that main constituents of pennyroyal oil were pulegone(100.0%), and spearmint oil were
, limonene(12.1%), carvone(85.4%). Among them, carvone and pulegone showed 100% fumigation at
air dose, respectively. It can concluded that two oils are potential control agents against F. occidentalis.
Occurrence of Culex pipiens (Diptera, Culicidae) and Effect of Vent Net Sets for Mosquito Control at Septic Tanks in South-eastern Area of the Korean Peninsula
Lee Dong-Kyu ;
Korean journal of applied entomology, volume 45, issue 1, 2006, Pages 51~57
Culex pipiens complex larvae were found at the septic tanks in Busan and Ulsan cities located in South-eastern area of Korean peninsula. The larvae were collected in 25, 35 and 75 septic tanks out of 3,527, 622 and 1,111 septic tanks which the breeding rates were averages of 0.7%, 5.6% and 6.8% in Busan from January to May, 2003; Busan from February to March, 2004; and Ulsan from January to April, 2005, respectively. The average numbers of the mosquitoes in the tanks were 38.8, 14.9 and 13.7 larvae/dip with a 355-ml dipper. The larval densities were from 25.4 larvae/dip in January to 43.7 larvae/dip in April. 2003 which were not significantly different between them. It was the first report to find mosquito breeding at septic tink in Republic of Korea. Culex pipiens larvae were found in 6 out of 379 septic tanks at complex buildings (over
in size) in Ulsan from June to September, 2005, having an average of 1.6% for mosquito breeding rate. The mosquito larvae were collected in 8 and 530 septic tanks out of 210 and 3,729 septic tanks at large (over 300 homes) and small apartments (below 300 homes) with 3.8% and 14.2% of the average breeding rates, respectively At the septic tanks of private houses for below 50 persons, 708 out of 7,178 septic tan had Cx. pipiens larvae and pupae with 9.9% of the average breeding rate. Total average of the mosquito breeding rate was 10.9% of the investigated septic tanks of residential buildings in Ulsan. The number of mosquito larvae in the septic tanks were 15 to 75 larvae/dip. The bent net sets of septic tanks prevented from mosquito breeding to the rate of 93.1%.
Distribution and Seasonal Occurrence of Japanese Gall-forming Thrips, Ponticulothrips diospyrosi, in Korea
Huh Wan ; Huh Hye-Soon ; Yun Ji-Eun ; Son Jun-Ki ; Jang Hyeon-Kyu ; Uhm Ki-Baik ; Park Chung-Gyoo ;
Korean journal of applied entomology, volume 45, issue 1, 2006, Pages 59~66
The damaged area by and the distribution of Japanese gall forming thrips, Ponticulothrips diospyrosi Haga et Okajima (Thysanoptera: Phlaeothripidae) in Korea were surveyed based on the answers of persimmon growers to questionnaire. Seasonal occurrence of each developmental stage was estimated by monitoring the individuals in damaged leaves, and effect of trap site and side of sticky board were studied by yellow sticky traps at three sweet persimmon orchards in Gimhae and Jinju (orchards A and B), Gyeongnam province, Korea in 2005. P. diospyrosi gave damage to astringent persimmon as well as sweet one. The damaged area of persimmon orchard has sharply increased since 2000, reaching 446 ha nationwide; Gyeongnam province was most severely damaged, followed by Gyeongbuk and Jeonnam provinces. Most of the farmers answered that they sprayed insecticides and removed the damaged leaves to control P. diospyrosi. The overwintered adults were observed in the damaged-rolled leaves from mid May and the 1st generation adults from early-mid June. No thrips were observed in the damaged leaves after mid July. Yellow sticky trap data showed that peak adults catch of the overwintered generation was mid May, and that of the 1st generation was mid-late June in Gimhae and late June in Jinju. Sticky traps set at orchard-pine forest border caught significantly more number of the 1st generation adults than those at the orchard center, even though timing of peak catches was not different between the two sites. There was a tendency of more catches on the side of sticky trap facing pine forest than the other side facing orchard center, even though the catches on both sides showed no statistical difference.
Effect of Turfgrass Height and Aeration on Pathogenicity of Entomopathogenic Nematodes to White Grubs in Golf Courses
Lee Dong-Woon ; Choi Woo-Geun ; Lee Sang-Myeong ; Choo Ho-Yul ; Kweon Tae-Woong ;
Korean journal of applied entomology, volume 45, issue 1, 2006, Pages 67~74
Korean entomopathogenic nematodes were introduced into cultural management of turfgrasses to enhance white grub control in golf courses for saving labour and expenses and contribution to giving safe playing and working places for golfers and superintendents by environmentally friendly control strategy. The turfgrass height influenced efficacy of entomopathogenic nematodes. Efficacy was higher at short turfgrass both in pot using Galleria mellonella larvae and in golf courses using 2nd instar of Exomala orientalis and Ectinohoplia rufipes as baits. Aeration increased the efficacy of entomopathogenic nematodes which were lower virulent. Pre-aeration was more effective than post-aeration. Exomala orientalis larval population was reduced 80.4 and 66.0% in the pre-aeration and post-aeration with entomopathogenic nematode, Steinernema carpocapsae Pocheon strain compared to 35.4% in the no aeration treatment.
Development of Pellet-type Artificial Diet for Lepidopteran Insect by Using a Twin Screw Extruder
Seol Kwang-Youl ; Hong Seong-Jin ; Kim Nam-Jung ; Kim Seong-Hyun ;
Korean journal of applied entomology, volume 45, issue 1, 2006, Pages 75~78
Development of pellet-type artificial diet for insect was tried by using a twin screw extruder(TSE). Screws were arranged several times and two reverse screws were equipped on the foreparts of 2 axes of TSE. Pellet-type diet could be produced successfully under conditions of TSE set as the following. : 300 rpm of main motor speed, 228 rpm of feed motor speed,
of running water speed and 5 mm of extrusion diameter. The optimum adding quantity of water to the manufactured diet was
times of dry diet. On the rearing results of beet armyworm, Spodoptera exigua and common cutworm, Spedoptera litura with the pellet-type diet, the final survival ratio (emergence rate) of these two species was over than 50%, and so it was concluded that the manufactured pellet-type artificial diet was sufficient dietetically to rear those insects.
Occurrence of Plant-parasitic Nematodes in Major Potato Production Areas and PCR Identification of Root-knot Nematodes
Cho Myoung-Rae ; Lee Young-Gyu ; Kim Jum-Soon ; Yoo Dong-Lim ;
Korean journal of applied entomology, volume 45, issue 1, 2006, Pages 79~85
This study was conducted to get basic information on the occurrence of plant-parasitic nematodes for the establishment of nematode management strategy in major potato production areas in Korea. Nationwide soil collection was done in 11 areas of Cheju, Yesan, Gimchun, Goryoung, Hong chun, Pyungchang, Gimjae, Milyang, Namwon, Gangnung, and Inje in 2004-2005. Root-hot nematode juveniles(J2) were detected in 30 samples among the 50 samples. The average density was 12-69 J2/100cc soil. Pratylenchus sp., Helicotylenchus sp., Ditylenchus sp., Tylenchus sp., and Tylenchorhynchus sp. were also detected in various locations, however, their densities were very low. Root-knot nematode females were collected from tomato roots inoculated with the potato field soils for PCR-RFLP identification. The females from Cheju, Milyang, and Goryung showed PCR products of 500 bp. And the Dra I restriction enzyme digestions showing 290 bp and 230 bp fragments confirmed their identity as Meloidogyne hapla.
Increased Attractiveness of the Aggregation Pheromone Trap of Bean Bug, Riptortus clavatus
Huh Wan ; Park Chung-Gyoo ;
Korean journal of applied entomology, volume 45, issue 1, 2006, Pages 87~90
The effect of food source in aggregation pheromone trap of bean bug, Riptortus clavatus, was evaluated in terms of the trap attractiveness using water-ran and cylinder-type traps. Water-pan trap baited with pheromone + food (dried soybean + dried peanut + water) attracted significantly more number of females and males than those with pheromone or control trap. Although cylinder-type trap baited with pheromone + food did not show significantly higher attractiveness, it attracted more adults and nymphs than the pheromone and control traps. Further research on the effect of trap types on attractiveness is needed.
Rearing system for rice leaffolder, Cnaphalocrocis medinalis (Lepidoptera: Crambidae) using corn seedlings
Park Hong-Hyun ; Park Chang-Gu ; Park Hyung-Man ; Uhm Ki-Baik ;
Korean journal of applied entomology, volume 45, issue 1, 2006, Pages 91~95
This paper reports a simple rearing system for Cnaphalocrocis medinalis based on corn seedlings diet. C. medinalis population under this system has been maintained by four stages (egg, young larva-1st to 3rd instar, old larva-4th to prepupa, 3nd adult) at
RH, 3nd 16L:8D photoperiod in a laboratory. We have elaborated a new egg collection method using a polystyrene container
which has made a great contribution in easy collection of eggs and storage of them for long period. Under this system, pupation and adult emergence rate, and pupal weight of C. medinalis population were close to the other reports by com seedling diet, and superior to those by artificial diets. Therefore, this rearing system would be useful in obtaining C. medinalsis population suitable to each specific needs.
Life Cycle of Tipula nova Alexander (Diptera: Tipulidae) under the Rearing Condition of Room Temperature
Kim Dong-Sang ; Lee Jong-Eun ;
Korean journal of applied entomology, volume 45, issue 1, 2006, Pages 97~100
Tipula nova appeared to have three generations a yew under the rearing conditions at room temperature. The first-generation with its eggs laid in April spent from 51 to 117 days, while the second-generation with its eggs laid in July spent from 57 to 93 days. The third-generation in which eggs were laid in September to grow until the following spring took 79 to 200 days. All the processes of life cycle of the species, when reared at room temperature from the spring to the summer with eggs deposited in the spring, were as follows: Eggs usually hatched between 7 and 10 days after oviposition. First instar larvae molted to the second instar in 7-10 days. Second instar larvae spent 7-12 days for next molting and third instar period lasted approximately 7-11 days. Fourth instar larvae spent 17-50 days for pupating. The duration of pupal stage was 3-6 days.