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Korean Journal of Horticultural Science and Technology
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Inheritance of Carotenoids Content in Red Pepper (Capsicum annuum L.) Fruit
Kwon, Oh-Yeol ; Yun, Hwa-Mo ; Park, Hyo-Geun ; Kim, Yong-Kwon ; Yun, Jae-Bok ;
Korean Journal of Horticultural Science and Technology, volume 24, issue 2, 2006, Pages 123~127
As a fundamental experiment for breeding chili pepper with high carotenoids content in red pepper, inheritance analysis for the trait was carried out using two different cross combinations between breeding lines of Korean chili pepper. The ASTA color value in the two F1 populations was a little low compared with the mean value of both parents, and the segregating populations of F2, BCP1 and BCP2 showed continuous distribution. The segregating pattern was quite similar in both cross combinations. These results indicated that the carotenoids content of powdered red pepper was inherited quantitatively, and the carotenoids content of both F1 populations seemed to have no heterosis and low additive gene effect. In spite of the fact that, the transgressive inheritance patterns of both BCP1 populations and somewhat high value of broad-sense heritability in both F2 populations were appeared to be that the possibility of breeding pepper with higher carotenoids content.
Enhanced Carotenoid Pigmentation during Room Storage of Red Peppers
Jung, Ji-Won ; Lee, Seung-Gu ;
Korean Journal of Horticultural Science and Technology, volume 24, issue 2, 2006, Pages 128~132
Peppers were harvested at faint red and deep red stages and stored at room temperature. Changes in surface color, carotenoid content, esterification degree, moisture and sugars were measured during storage. Reflected color attributes (L, chroma, hue angle) were higher in fruits of faint red than of deep red, whereas the carotenoid content and esterification degree were lower in fruits of faint red at harvest. After 6 days of storage, reflected color attributes of fruits harvested at faint red decreased with a rapid increase of carotenoid content and esterification degree, and as a consequence, there was no significant difference between both fruits harvested at different ripeness stage. However, chroma, hue angle and carotenoid content showed a difference after 12 days of storage. These were caused by a decrease in nonesterified carotenoids of fruits harvested at faint red as compared with fruits harvested at deep red. Moisture content and sugars were found to be similar in both stages during storage, as well as at harvest.
Line Selection Effects by Single Seed Descent with Different Population Size in Cherry Tomato (Lycopersicon esculentum var. cerasiforme Alef.)
Park, Kwon-Seo ; Yun, Hwa-Mo ;
Korean Journal of Horticultural Science and Technology, volume 24, issue 2, 2006, Pages 133~137
This study was conducted to investigate the effects of the population sizes on the line selection by single seed descent (SSD) breeding method in cherry tomato. The seed were obtained from crossing for ‘Mini Carol’ and ‘Dadagi’. The population groups were separated for 30, 60, 120, and 210 seeds group. Soluble solids contents were higher and fruit weights were more decreased in late generation (G5) than in early generation (G2). In addition, the appearance ratio of the plants with sweet fruits was increased with the generations advanced and soluble solid was ranged from 8.3 to 8.8 oBrix. By these results, it is expected to be able to select the lines with sweeter fruits than those of parents. The selection effect was low in the population with 30 plants and it was same in three popuiations 60, 120 and 210.
Effect of Automatic Ventilation of Greenhouse During Daytime on the Growth and Wilting Occurrence in Watermelon
Kwon, Jun-Guk ; Kang, Kyeong-Hui ; Choi, Young-Ha ; Lee, Jae-Han ; Kwon, Ki-Beom ; Kang, Nam-Jun ; Lee, Han-Chul ;
Korean Journal of Horticultural Science and Technology, volume 24, issue 2, 2006, Pages 138~142
This study was performed to investigate the effect of automatic ventilation based on greenhouse air temperature on the growth, fruit quality and wilting occurrence of greenhouse watermelon (Citrullus vulgaris) at annually wilting field of a chief producing district, Haman, from 2002 to 2004. The automatic ventilation with setpoint 35℃ and the control (mostly close) of greenhouse during the daytime were compared. Automatic ventilation produced around 3℃ low air temperature and 7% low air humidity in greenhouse during the daytime, and thus vigorous growth, 10 to 20% heavier fruit and reduced wilting occurrence, compared to those of the control. Results indicate that automatic ventilation could be effective in temperature management for high productivity and low wilting occurrence of greenhouse watermelon in low temperature season.
Effect of Thermal Tunnel Covers and Warm-Water Heating Using Excel Pipe on the Growth and Wilting of Greenhouse Watermelon
Kwon, Jun-Kuk ; Choi, Young-Ha ; Lee, Jae-Han ; Kang, Kyeong-Hee ; Kwon, Ki-Beom ; Kang, Nam-Jun ;
Korean Journal of Horticultural Science and Technology, volume 24, issue 2, 2006, Pages 143~147
Effects of thermal tunnel covers and warm-water heating using excel (XL) pipe on the growth, yield, and wilting of greenhouse watermelon were investigated at annually wilting field of a chief producing district, Haman. Thermal tunnel covers (four, eight and twelve ounces) and warm-water heating setpoints (15℃ and 20℃) with laying XL pipes on the soil surface during the night time were compared, respectively. High plant dry matter and fruit yield and low wilting occurrence were observed in heavier thermal cover but the difference between eight and twelve ounce was not significant. Warm-water heating of 20℃ setpoint increased 3.5℃ in air temperature and 800 g in fruit weight, and reduced 20% in wilting occurrence, compared to those in non-heating. Results indicate that eight to twelve ounces of thermal tunnel cover and warm-water heating using XL pipe could be some effective in temperature management for high productivity and low wilting occurrence of greenhouse watermelon in low temperature season without excessive fuel consumption.
Effect of Liquid Pig Manure on Growth, Nutrient Uptake of Onion, and Chemical Properties in Soil
Lee, Jong-Tae ; Ha, In-Jong ; Kim, Hee-Dae ; Mun, Jin-Sung ; Kim, Woo-Il ; Song, Won-Du ;
Korean Journal of Horticultural Science and Technology, volume 24, issue 2, 2006, Pages 148~156
This study was carried out to evaluate the effect of liquid pig manure (LPM) on the nutrient uptake, yield of onion and soil properties. The LPM applied to onion field contained 4.2 g kg-1 N, 0.34 g kg-1 P, 1.2 g kg-1 K and it was diluted with water by 1:2 for top-dressing, which was applied separately 4 or 6 times from February to April. The LPM application rate for basal fertilization was 19,280 kg ha-1 which included 80 kg N, 6.6 kg P, and 22 kg K. The application rate for top-dressing was 101,910 kg ha-1 which included 160 kg N, 13 kg P, and 53 kg K. The plant height was not almost different among treatments until 136 days after planting. Thereafter, it enlarged abruptly until 186 days, on May 4. The significant difference among CF and LPM + top-dressing by CF or LPM I was not shown, but no fertilizer and LPM only (it was applied totally as basal fertilization) made plant height of onion lessen at the maximum growth stage because of insufficient nutrients. The plant diameter increased after 136 days, and there was a significant difference between CF or LPM and no fertilizer from 186 days after planting. The leaf weight was similar tendency to plant height while bulb weight was similar to plant diameter. The amount of nutrient uptake in leaf coincided with leaf growth and the nutrient uptake in bulb swelled simultaneously with bulb growth. The difference between CF and LPM + top-dressing by CF or LPM I was not significant in nutrient uptake of onion leaf and bulb. But LPM only decreased in nitrogen and potassium of leaf and in nitrogen of bulb. And no fertilizer made a remarkable difference to others in nitrogen, potassium and magnesium of leaf and bulb. The utilization of nitrogen and phosphorus in LPM + top-dressing by CF were 23.5%, 13.2% respectively, more than the others. The LPM + top-dressing by LPM I was most available with potassium, and it leaded the most abundant marketable yield by 55.0 t ha-1 as well. The nitrogen, phosphorus, and potassium contents in bulb were inclined to decrease with progress of onion growth. The nitrogen contents in leaf maximized at the initial growth stage while potassium contents increased at the initial bulbing stage. The LPM application increased pH, nitrate nitrogen, potassium and EC in soil more than chemical fertilizer (CF) did.
Effect of Double Layered Substrate on the Growth, Yield and Fruit Quality of Strawberry in Elevated Hydroponic System
Jeon, Ha-Jun ; Lee, Ki-Myeong ; Hwang, Jin-Kyu ; Kim, In-Kyu ; Son, Mi-Ja ;
Korean Journal of Horticultural Science and Technology, volume 24, issue 2, 2006, Pages 157~161
This experiment has investigated the effects of growth, yield, and fruit quality of strawberries by two different kinds of growth substrate layers. The experiment was performed with strawberry cultivar ‘Akihime’ in greenhouse in Daegu University. Perlite, cocopeat, cocopeat/perlite (upper layer/lower layer), vermiculite/perlite and the mixture of vermiculite and rice hull were used for solid substrate. Nutrient solution for strawberries, which made by Yamazaki, was supplied in proper concentration by its growth stage. Early stages of strawberry growth were the best at the cocopeat substrate in terms of number of leaves, leaf length, and leaf width. The soluble solids and fruit length of the strawberries did not differ from the different kind of substrates. However, the weight and diameter of strawberries did have the best outcomes from the cocopeat substrate and the cocopeat/perlite two layer substrates. The number of strawberries was the best at the cocopeat/perlite substrates due to the high capacity of moisture holding at the upper layer and the good air permeability of the lower layer, which derived good nourishment from the well developed roots. The results of this experiment will be utilized in the new substrate application for strawberry hydroponics.
Growth and Fruit Characteristics as Affected by Different Planting Distances among Plant Types in Pepper (Capsicum annuum L.) for Pickles
Lee, Su-Yeon ; Lee, Sang-Woo ; Im, Jae-Wook ; Sim, Sang-Yeon ; Lee, Hae-Kil ;
Korean Journal of Horticultural Science and Technology, volume 24, issue 2, 2006, Pages 162~167
This experiment was conducted to determine planting distance of jalapeo pepper (Capsicum annuum L. cv. Sierra Fuego) and 3 types of plant attitude in pepper for pickles. Jalapeo pepper was grown at in-row spacings of 30, 45, 60, and 70 cm. To compare the growth and fruit characteristics among the different plant types, erect, semi-spreading, and spreading were used in this study, and those were grown at in-row spacings of 20, 30, and 45 cm. In jalapeo pepper, stem diameter was gradually thicken as increasing in-row spacing, but another growth characteristics such as plant height, leaf length and width, and main stem length were not different among in-row spacings. External characteristics and color degree of jalapeo pepper fruit were not different among in-row spacings. The yield per plant increased as increasing in-row spacing, however, yield per unit area increased as decreasing in-row spacing with the highest at 30 cm. There showed no difference at nutrient contents in various in-row spacing. In different plant types, plant height showed no difference among in-row spacings, but stem diameter increased as increasing in-row spacing. There were not significant difference in fruit characteristics such as fruit length and width, sugar contents, fruit hardness, and color degree among in-row spacings, but fruit length was long, and fruit width diminished as decreasing in-row spacing. The number of fruits and yield per plant increased as increasing in-row spacing, but the total yield in unit area was the highest at the lowest in-row spacing of 30 cm regardless of plant type.
Changes in Fruit Characteristics after Brining of Pickling Pepper (Capsicum annuum. L) Cultivars
Lee, Su-Yeon ; Lee, Sang-Woo ; Lee, Yong-Beom ; Sim, Sang-Yeon ; Seo, Myeong-Hun ; Im, Jae-Wook ;
Korean Journal of Horticultural Science and Technology, volume 24, issue 2, 2006, Pages 168~176
The purpose of this research was to determine changes in characteristics after brining fruits of various pepper cultivars. Peppers were 7 cultivars which were ‘Sierra Fuego’ and ‘Umgi’ in jalapeo type, ‘Piccante a Mazzetti’ in cherry type, ‘Robustini’ and ‘Serenade’ in ancho type, and ‘Peperone thor’ and ‘Corno di Toro Giallo’ in wax type. After brining, the amounts of water and EDTA soluble pectins were lower in cv. ‘Sierra Fuego’ and ‘Umgi’ than the others, and were the highest in ‘Robustini’. Insoluble pectins were the highest in cv. ‘Sierra Fuego’, and decreased in all cultivars after brining. Capsaicin content increased in all cultivars after brining, and were higher in cv. ‘Piccante a Mazzetti’ than other cultivars. Capsaicin contents were quitely different among 7 cultivars. Lightness (L) degree of fruit went down in all cultivars after brining. The degree of greenness (a) has been fallen off, but yellowness became higher after brining in cv. ‘Sierra Fuego’ and ‘Umgi’ as green type cultivars. In the red type of cultivars, color degree was not different between fresh and brined fruit. Sensory characteristics by panel test were texture, color, and taste of brined pepper, and good scores were gotten in ‘Sierra Fuego’, ‘Umgi’, and ‘Piccante a Mazzetti’. Free sugar contents were the highest in ‘Robustini’ as 28.5%, and were the lowest in ‘Peperone thor’ as 7.9%. After brining, total free sugar contents were not different in cultivars.
Effect of Wood Vinegar and Charcoal on Growth and Quality of Sweet Pepper
Jeong, Chun-Sun ; Park, Jong-Nam ; Lee, Seong-Jae ; Jo, Tae-Su ; Yun, In-Ju ; Jeong, Jang-Hyeon ; Kang, Jung-Pil ; An, Byeong-Jun ;
Korean Journal of Horticultural Science and Technology, volume 24, issue 2, 2006, Pages 177~180
This study was conducted to investigate effects of physiological characteristics and fruit quality by treatment of wood vinegar and charcoal during culture of sweet pepper. Plant height, chlorophyll content and photosynthetic rate were appreciated in only wood vinegar and mixed treatment (wood vinegar+charcoal powder). Fruit length, fruit diameter and pericarp thickness, estimating the quality were very effective in mixed treatment. Percentage of marketable fruit was also the greatest in mixed treatment. Contents of soluble soild, sugar, and ascorbic acid content were the highest in mixed treatment and followed by treatments of wood vinegar, charcoal powder, and control.
Effect of KCl or K2SO4 Supplement to Nutrient Solution on Yield and Fruit Quality in Sweet Peppers (Capsicum annuum 'Special' and 'Fiesta')
An, Cheol-Geun ; Jeong, Byeong-Ryong ; Yun, Hye-Suk ; Hwang, Hae-Jun ; Hwang, Yeon-Hyeon ; Son, Kil-Man ; Song, Keun-Woo ;
Korean Journal of Horticultural Science and Technology, volume 24, issue 2, 2006, Pages 181~189
This study was carried out to reduce fruits with uneven-coloration, which is a main attribute to low quality fruits in sweet pepper. To improve fruit coloration, 1, 2, or 4 mM KCl or 2 mM K2SO4 were supplied to the nutrient solution, and plant growth and fruit quality were compared with those in the control, the Grodan standard nutrient solution treatment. Levels of N, Ca, and Mg in leaves decreased, while K and Cl increased, as compared to those of the control. With the promoted uptake of K and Cl as well as the suppressed uptake of N by the KCl or K2SO4 supplement to the solution, fruit content of capsanthin and hunter value of fruit color increased, while chlorophyll content decreased, as compared to the control. As a result, percentage of unevenly colored fruits, decreased by nearly 6%. Although plant growth was slightly suppressed, yield was not affected by these treatments.
Breeding of Cluster Type Tomato 'Geumgwan Gaya' for Protected Cultivation
No, Il-Rae ; Jeong, Jae-Wan ; Kang, Kyeong-Hee ; Jeong, Hae-Bung ; Lee, Seong-Chan ;
Korean Journal of Horticultural Science and Technology, volume 24, issue 2, 2006, Pages 190~193
Geumgwan Gaya’ is a new variety of cluster-type tomato for protected cultivation. This cultivar was developed by crossing two inbred lines, ̒Wonung No. 1’ of male sterile line and ‘(G/MY)-6-28-4-37- 6-54-9’ bred following the pedigree breeding method. ‘Geumgwan Gaya’ is an indeterminate type. It has heart shaped fruit with red flesh and colorless skin. The number of locule are 2 or 3. The soluble solid contents of this cultivar is 7.0 °Bx above. But this cultivar was susceptible to tomato mosaic virus, fusarium wilt and leaf mold disease. it can be cultivated in the protected cultivation area everywhere in Korea.
Breeding of Full-Ripe Tomato 'Tamsrye' for Protected Cultivation
Jeong, Hae-Bung ; Jeong, Jae-Wan ; Kang, Kyeong-Hee ; No, Il-Rae ; Lee, Seong-Chan ; Kim, Hui-Tae ;
Korean Journal of Horticultural Science and Technology, volume 24, issue 2, 2006, Pages 194~197
‘Tamsrye’ is a newly bred full-ripe type tomato cultivar for protected cultivation. This cultivar is a F1 hybrid variety originated from the cross of ‘Tamnara’ and (T/MY)-98-21-5-28-6-32-348. ‘Tamsrye’ is an indeterminate type. The fruit shape is round flat and the skin color is yellow with red flesh. The number of locule are 3 or 4. The soluble solid contents is about 6.0 °Bx. The average fruit weight of ‘Tamsrye’ is about 180 g and the marketable yield is relatively high because of low percentage of abnormal fruit bearing. But this cultivar is susceptible to tomato mosaic virus, fusarium wilt and leaf mold disease. This cultivar can be cultivated in the protected cultivation area everywhere in Korea.
Persistence of Entomopathogenic Nematode, Steinernema carpocapsae GSN1 Strain on Vegetable Leaf in Greenhouses
Kim, Hyeong-Hwan ; Jeon, Heung-Yong ; Lee, Dong-Un ; Chu, Ho-Ryeol ; Jo, Seong-Rae ;
Korean Journal of Horticultural Science and Technology, volume 24, issue 2, 2006, Pages 198~204
Persistence of the entomopathogenic nematode Steinernema carpocapsae GSN1 strain applied by foliar sprays was evaluated on the leaves of Chinese cabbage, cabbage, and kale in greenhouses. Kinds of vegetables, seedling age, and application time influenced persistence of S. carpocapsae GSN1. More S. carpocapsae GSN1 survived on Chinese cabbage leaf than on cabbage or kale and persisted longer on older leaves than younger leaves. S. carpocapsae GSN1 did not survive for 6 hour at the applications of 08:00 and 14:00 while persisted 12 hour at the application of 18:00 hour. The survial rate of S. carpocapsae GSN1 sprayed 200,000 and 400,000 infective juveniles (Ijs) was 3.5 times higher than 100,000 Ijs. For the Chinese cabbage, the suitable water volume for the application of S. carpocapsae GSN1 was 150 mL per m2, which was better for the survival of nematodes than 300 and 600 mL per m2.
Fruit Quality of Once-over Harvest Pepper (Capsicum annuum) Cultivar 'Saengryeg No. 211' and 'Saengryeg No. 213'
Jeong, Ji-Won ; Jo, Yong-Seop ; Jo, Myeong-Chul ;
Korean Journal of Horticultural Science and Technology, volume 24, issue 2, 2006, Pages 205~209
The characteristics of pepper fruit (Capsicum annuum), ‘Saengryeg No. 211’ and ‘Saengryeg No. 213’ bred for onceover harvest were compared to those of other commercially available cultivars. Yield, weight, height, and width of fresh fruits were evaluated at red stage. Firmness and contents of capsaicinoids, vitamin C, tocopherol, and sugars were measured in green and red stages. Yield and ASTA value of ‘Saengryeg No. 211’ and ‘Saengryeg No. 213’ were found to be in the range of commercial cultivars. Fresh fruit weight and height were lowest in ‘Saengryeg No. 213’. The capsaicinoid content of ‘aengryeg No. 211’ was higher than any other examined cultivars, whereas that of ‘Saengryeg No. 213’ was not detected in both green and red stages. Firmness of ‘Saengryeg No. 213’ was lower than any other varieties in both green and red stages. Other characteristics of quality in ‘Saengryeg No. 211’ and ‘Saengryeg No. 213’ were similar with examined commercial cultivars in both stages.
Fruit Quality, Yield, and Profitability of 'Hongro' Apple as Affected by Crop Load
Jo, Kwang-Hyun ; Yun, Tae-Myeong ;
Korean Journal of Horticultural Science and Technology, volume 24, issue 2, 2006, Pages 210~215
Fruit load on 5-year-old ‘Hongro’/M.9 (3.5×1.0 m in planting distance) apple was adjusted to 3, 4, 5, or 6 fruits per trunk cross-sectional area (TCA) cm2 in 2003, and to 4, 5, or 6 fruits in 2004, in oder to find the optimum fruit load level from the viewpoint of profitability in case of farmer-to-consumer direct sale. Vegetative growth such as TCA increment and shoot length per fruit weight was reduced, but yield per tree significantly increased by increasing crop load level. Average fruit weight decreased from 309 g in 3 fruit load to 241 g in 6 fruit load in 2003 and from 270 g in 4 fruit load to 241 g in 6 fruit load in 2004, while yield per tree increased from 18.7 to 27.8 kg in 2003 and from 25.5 to 32.6 kg in 2004. Large fruit ratio increased significantly, and soluble solid contents and color development of fruits were more or less improved by lowing the crop load. Return bloom was enough for ordinary fruit set even in the highest crop load in the previous year. Crop load of 5 fruits per TCA cm2 generated the highest income in case of farmer-to-consumer direct sale, 18,471 thousand won per 10a in 2003 and 21,116 thousand won in 2004. In conclusion, because apple consumer prefers taste, color, safety, and confidence to fruit grower to fruit size in case of direct sale to consumer, about 100 fruits per tree in full productive age, or 5 fruits per TCA cm2 in 5 to 6 years old tree, in high density ‘Hongro’ growing was optimal crop load for high income which could bring higher yield with more or less smaller fruits, compared to lower fruit load.
Ripening Responses and Quality Changes of 'Fuyu' Persimmon Fruit as Influenced by Exogenous Ethylene and Subsequent Short-term Storage Temperature
Park, Yun-Mun ; Lee, Yong-Jae ;
Korean Journal of Horticultural Science and Technology, volume 24, issue 2, 2006, Pages 216~221
‘Fuyu’ persimmon fruits were harvested at commercial maturity, exposed to exogenous ethylene for one day, and then put on the shelf under different temperature conditions to elucidate physiological response and postharvest quality changes. Effects on ripening events were discriminated between ethylene treatment and shelf temperature through two-way analysis of variance. Exogenous ethylene at 10 μLL-1 level induced significantly higher ethylene evolution and respiration rates, whereas effects of 1 μLL-1 ethylene treatment were slight. Level of exogenous ethylene to induce ethylene production and respiratory changes appeared to be in the range of 1 and 10 μLL-1. Unlike physiological metabolism, flesh firmness was significantly reduced even by 1 μLL-1 ethylene treatment indicating that critical exogenous ethylene level hastening cell wall metabolism as related to fruit softening seemed to be lower than 1 μLL-1. Effects of storage temperature were significant on the changes in respiration rates, while the effects were minimal and occasional on the changes in ethylene evolution and flesh softening. Coloration, in contrast, was influenced by both the external ethylene and storage temperature at the similar significant level. Effects of the two factors on the changes in soluble solid concentrations were not consistent. Responsiveness to exogenous ethylene and storage temperature in ‘Fuyu’ persimmon may differ according to respective ripening events.
Characteristics of Flower Bud Differentiation in Highbush Blueberry (Vaccinium corymbosum L.) Cultivars
Bae, Kang-Sun ; Kim, Tae-Chun ; Lee, Hee-Jae ; Lee, Byeong-Il ; Kim, Ho-Chul ;
Korean Journal of Horticultural Science and Technology, volume 24, issue 2, 2006, Pages 222~227
Characteristics of flower bud differentiation in highbush blueberry (Vaccinium corymbosum L.) cultivars were investigated. The blueberry buds were burst from late March to mid April in Iksan area. The buds of ‘Sharpblue’, blueberry in Southern type, swelled on March 23, while those of ‘Jersey’ and ‘Sunrise’ did on April 13. Their blooming in Iksan area started from mid April to late May. ‘Sharpblue’ and ‘Jersey’ were the earliest (April 16) and the latest (April 25) blooming cultivars, respectively. Shoot resting dates of the cultivars, as estimated by the apical bud wilting and tip blackening, ranged from early May to mid July. Since the axillary buds enlarged and took the form of terminal buds, they were regarded as pseudoterminal buds. The flower buds were not changed morphologically until late August, but subsequently became larger and developed numerous florets. Thus, the flower bud differentiation was thought to occur late August. Flower buds were formed at the second or third node from the apical bud. Number of flower buds on short length shoot and shoot of 10～30 cm length was from 1 to 2 and from 2 to 3, respectively. Number of florets per flower bud was difference as that was from 6.8 to 11.0 and ‘Bluecrop’ and ‘Dixi’ were very large in coefficients of variation.
Identification of Fruit-dominant cDNAs in a Ripened Citrus (Citrus unshiu) Using Differential Screening
Jeon, Oh-Sung ; Heo, Yun-Kang ; Kim, In-Jung ;
Korean Journal of Horticultural Science and Technology, volume 24, issue 2, 2006, Pages 228~235
Fruit ripening is a specialized process that can not be observed in other plant organ. Research about genes relating to major characteristic of fruit such as color, sugar content, and aroma is required to understand the ripening process and to breed new cultivar by molecular engineering. Through differential screening between leaf and fruit mRNA, we isolated six genes including Lea (late-embryogenesis-abundant) protein, pyruvate kinase, signal recognition particle protein, dehydrogenase and two unknown genes, whose transcripts were dominantly detected in the fruit. The expression analysis showed stronger signal in fruit than in leaf, which is matched to the results of differential screening. The results suggest that fruit-dominant genes are related to the physiological functions including stress-responsive and sugar metabolism in fruits.
Stimulation Effect of Environment-Friendly HrpNEp Protein on Growth and Cut Flower Characteristics of Oriental Hybrid Lily
Kim, Hak-Ki ; Park, Deok-Hwan ; Im, Chun-Geun ; Hong, Se-Jin ; Lee, Mi-Rim ; Eom, Byeong-Heon ;
Korean Journal of Horticultural Science and Technology, volume 24, issue 2, 2006, Pages 236~242
This study was conducted to investigate the effect of HrpNEp protein extracted and purified from Erwinia pyrifoliae WT3 on the growth and cut flower quality of oriental hybrid lily. HrpNEp, a kind of environment-friendly protein, stimulated growth of the shoot length and stem diameter, and increased the stem strength, but decreased the curvature of the shoots with flower buds. Calcium and magnesium which are supposed to have some positive effects on the hardness of tissues were absorbed more in HrpNEp-treated shoots than the control. To improve the quality of cut flowers, the lily needed 4 or 2 times treatments with 10 or 40 mgL-1 HrpNEp, respectively, and additional treatment with HrpNEp gave even better quality. There was some antagonism between magnesium and calcium when they were used alternatively with HrpNEp. High quality cut flowers were produced by treatment with 10 mgL-1 HrpNEp and calcium, with a synergistic effect within the limits of antagonism, and also with 40 mgL-1 HrpNEp treatment alone.
Effective In Vitro Propagation of Narcissus pseudonarcissus through Hypocotyl Culture
Go, Jeong-Aae ; Kim, Hyeong-Mu ; Kim, Hyun-Sun ; Kim, Myeong-Jun ; Lee, Eun-Jeong ; Choi, Jeong-Ran ;
Korean Journal of Horticultural Science and Technology, volume 24, issue 2, 2006, Pages 243~247
수선의 효율적 기내번식을 위해 MS 기본배지에 2,4-D, NAA, kinetin 및 BA 다양하게 조합하여 배축을 배양하였다. 절편체 부위와 식물생장조절물질은 캘러스, 자구형성 및 식물체 기관분화에 상당한 영향을 미쳤다. 배축 기부로부터 2.0cm 길이는 배양에 가장 적합하였고, 캘러스유도 및 자구 형성을 위한 2,4-D 적정농도는 2.0mgL-1 이었다. NAA와 BA 혼용처리구에서 분화된 캘러스 및 자구는 2,4-D와 kinetin 혼용구에서 형성된 신초수에 비해 1.5-2.0배 증식효과가 있었다. 1.0mgL-1 NAA와 1.0mgL-1 BA 혼용처리는 65%이상 자구가 형성되어 가장 효과적이었다. 개체별로 분리한 자구 및 신초는 0.5mgL-1 NAA처리에서는 자구 비대와, 0.01mgL-1 IAA처리에서 뿌리 분화에 가장 양호하였다.
Plant Regeneration According to Culture Conditions in Leaf Culture of Drosera spatulata sp. Tokaiensis
Kim, Seong-Jin ; Jo, Gyeong-Chul ; Kim, Jeong-Geun ; Hwang, In-Taek ; Kim, Su-Ok ; Kim, Kwang-Su ;
Korean Journal of Horticultural Science and Technology, volume 24, issue 2, 2006, Pages 248~252
In order to establish in vitro propagation of sundew, Drosera spatulata sp. Tokaiensis, the effect of MS medium, sucrose, activated charcoal concentration on shoot proliferation and root formation were investigated using leaves at 6 months after seed germination. Survival rates of leaf explants were higher in dark condition than light condition and as low as lower MS medium strength. Shoot and root growth were better in quarter strength of MS medium than that of other MS media, but were not different significantly according to light condition. Survival rate accroding to activated charcoal concentration was higher in 1/4 MS medium than those of 1/2 MS and 1 MS media, but was not different significantly. In the whole treatments, the optimum sucrose concentration for shoot proliferation was over 30 gL-1. Therefore, to increase the proliferation efficiency through leaf culture of sundew, it might be cultured in quarter strength of MS medium with 30 gL-1 sucrose.
Selection of RAPD Primers for Efficient Fingerprinting in Roses
Kim, Ki-Jun ; Choi, Jeong-Geun ; Im, Ki-Byeong ; Han, Tae-Ho ; Ki, Kwang-Yeon ;
Korean Journal of Horticultural Science and Technology, volume 24, issue 2, 2006, Pages 253~257
This study was conducted to select random amplified polymorphic DNA (RAPD) primers for showing reliable polymorphism in roses. The rose genomic DNA concentration for polymerase chain reaction (PCR) was from 1 to 20 ng and annealing temperature was from 35 to 40℃. Taq polymerase concentration was efficient in 1.0 unit. Band patterns were distinguishable in 155 primers out of 191 primers and the 100 primers were reproducible. Twenty primers were selected with distinguishable and over 80% reproducible polymorphism on agarose gel. This study will support Korean rose breeding program and the fingerprints obtained in this study were uploaded on the internet site; http://hanth.jnu.ac.kr.
Inheritance of Qualitative Character in Primula polyantha
Song, Chun-Young ;
Korean Journal of Horticultural Science and Technology, volume 24, issue 2, 2006, Pages 258~265
This experiment was conducted to determine the inheritance of the flower color, flower size, petal shape, leaf color, and leaf shape in Primula polyantha. The cross combination (F1) by pure line (S7), its self-pollination (F2) and backcross were tested by segregation. The petal color was inherited a monogenic complete dominance by progenies (F2) and backcross with Yellow × White (J × F) and Yellow × Red (J × B). However, the combinations of Yellow × Pink (J × D) and Purple × White (C × F) showed a monogenic partial dominance. Based on Mendelism analysis, it was found that petal color was inherited a complete or partial dominant pattern. The big size of petal, wave of petal shape, dark green of leaf color and oval of leaf shape were inherited a monogenic complete dominance by testing of progenies (F2) and backcross.
Seed Formation by Crossing Time, Methods and Stigma Position of Primula polyantha
Song, Chun-Young ;
Korean Journal of Horticultural Science and Technology, volume 24, issue 2, 2006, Pages 266~271
This experiment was carried out to evaluate of seed formation by crossing time, methods, and stigma position with 45 inbred lines of Primula polyantha. By the stigma position, the ripening seed capsule of Thrum style was low to 53.3% and its seed number per capsule was also low to 22.9. The Pin style was much more ripening seed capsule to 80.6% and number of seed per capsule than that of Thrum. And the Homo style was formed 94.8% of ripening seed capsule and number of seed in a capsule to 27.5. The ripening seed capsule of selfing was low to 44.3% and its seed number per capsule was also low to 9.5. The sib crossing was much more ripening seed capsule and number of seed per capsule than that of selfing in the lines. However, the outcrossings were formed 83.6% of ripening seed capsule and increased number of seed in a capsule ranged 18.2 to 47.5 compared to selfing or sib crossing. The ripening seed capsule and number of seed in a capsule within 10 day’s crossings after flowering very low, however, the crossings after 10 to 20 days from flowering in the lines were well formed the seed. The big size of stigma head and plenty quantity of pollen in a stamen head were well formed ripening seed capsule and high number of seed in a capsule.
In Vitro Propagation of Korean Native Winter Hazel (Corylopsis coreana)
Ko, Gap-Cheon ; Im, Dong-Ok ;
Korean Journal of Horticultural Science and Technology, volume 24, issue 2, 2006, Pages 272~278
This study was carried out to propagate an endangered Korean winter hazel (Corylopsis coreana) in vitro. It was effective to do suspension-culture 2 mm shoot tips in size on the MS liquid media containing 2 mgL-1 6-benzylaminopurine (BA) for establishing shoots in vitro. Shoots were well multiplicated and grown when subcultured on the MS media containing 1~2 mgL-1 BA + 0.1 mgL-1 α-naphthaleneacetic acid (NAA), while shoots were over-multiplicated when on the MS media containing more than 3 mgL-1 BA. A two-step culture method was used for the rooting of shoots in vitro. In the first rooting step, 1 cm shoot tips or nodes with one leaf were cultured on the 1/4 MS media containing NAA or indole-3-butyric acid (IBA) for 6 days in the dark. Then, they were cultured on 1/4 MS media without growth regulators. The best rooting, 80~100%, was obtained from the shoot tips cultured on the 1/4 MS media containing 1~3 mgL-1 IBA in the first rooting step. The rooted plantlets were acclimatized in the closed box, and then grown in the plastic house.
Effects of Scale Position and Cutting Condition on Bulblet Growth in Perforated Polyethylene Film Bag in Scaling of Lilium longiflorum 'Gelria'
Yu, Yong-Kwon ; Kim, Byeong-Woon ;
Korean Journal of Horticultural Science and Technology, volume 24, issue 2, 2006, Pages 279~284
This study was carried out to examine the effect of scale position and cutting condition on bulblet growth in perforated polyethylene film bag in scaling of Lilium longiflorum ‘Gelria’. Cutting with scales obtained from outer position of mother bulb was more effective in bulblet formation and growth than those from inner or middle position. Weight, diameter, height, and number of bulblets were increased at 25℃ as compared to 15, 20 or 30℃. Addition of 300～400 mL water per 1 L medium increased the weight of bulblet by 80% as compared to the control. Scaling media were the best in peatmoss for bulblet growth. Mixing of scales and medium at 1:4 ratio was effective for scaling of Lilium longiflorum ‘Gelria’ in perforated polyethylene film bag.
In Vitro Bulblet Induction and Plant Regeneration from Immature Zygotic Embryos Cullture of Lilium tsingtauense Gilg.
Korean Journal of Horticultural Science and Technology, volume 24, issue 2, 2006, Pages 285~289
A system for multiple bulb scale formation and plantlet regeneration from immature embryos explants of Lilium tsingtauense Gilg. cultured on MS medium containing NAA/BA and 2,4-D/BA was established. Developmental stage of embryos and plant growth regulators significantly affected on callus and bulb scale induction. The globular and torpedo stage of embryos did not callus and bulb scale induction. The optimal stage of embryos was early cotyledonary stage of embryos in 70-90 days after pollination. In culture of immature embryos with cotyledonary stage, organogenic callus induced and led to bulb, shoot and root formation. Explants on medium with NAA/BA produced higher number of bulb scales than with 2,4-D/BA. Sixty-seven percent of explants was produced when explants were cultured with a combination of 1.0 mgL-1 NAA and 1.0 mgL-1 BA for 30 days. The highest number of bulb scale (12.0 to 15.0 bulb scales per explant) was obtained when explants were treated with a combination of 3.0 mgL-1 NAA and 3.0 mgL-1 BA for 60 days culture. The number of shoots formed from the bulb scales by transferred to the medium with 2.0 mgL-1 NAA and 2.0 mgL-1 BA. Bulb scale disks formed roots in MS medium without plant growth regulators. A total of 90 plants were regenerated from explants, morphological abnormalities were not observed among regenerated plants. Regenerants were transferred to the pot and grown to maturity.
Microspore-derived Embryogenesis and Plant Regeneration from Anther Culture of Pulsatilla cernua var. koreana
Ko, Jeong-Aae ; Kim, Hyun-Sun ;
Korean Journal of Horticultural Science and Technology, volume 24, issue 2, 2006, Pages 290~295
Microspores of Pulsatilla cernua var. koreana (Ranunculaceae) which could produce embryogenic calli or embryos were cultured on Murashige and Skoog’s medium with different kinds of plant growth regulators. Three different pathways could be recognized in this process; the first involving the main vegetative cells, the second containing all the generative and vegetative cells, and the third starting with two equal cells in the pollen grains. Androgenesis initiation was highest when anthers contained pollen at the uninucleate stage or early binucleate stage 4-5 days before anthesis. MS medium with 0.5 mgL-1 NAA and 1.0 mgL-1 BA was found to be highly desirable for the induction of androgenesis in this plants. Addition of 10% coconut water to the culture medium seemed to significantly improve callus or embryo production. The calli developed buds and subsequently regenerated plantlets. Shoot multiplication and elongation were also increased by adding 10% coconut water. Rooting occurred rarely from the plantlets that formed from the pollen embryos. However, 5% of roots from shoot were produced on MS medium with 0.5 mgL-1 NAA and 1.0 mgL-1 BA.