Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Horticultural Science and Technology
Journal Basic Information
Journal DOI :
Korean Society of Horticultural Science
Editor in Chief :
Volume & Issues
Selecting the target year
Effect of Plant Growth Regulators on Growth and Quality of Fruits in Triploid Hybrid Grapes
Park, Sung-Min ;
Korean Journal of Horticultural Science and Technology, volume 28, issue 1, 2010, Pages 1~7
This study has been conducted to investigate the effect of gibberellic acid and several plant growth regulators on growth and quality of fruits in triploid grapes. The gibberellic acid (GA
, 50 and 100 mg L
) and GA3 (100 mg L
) with other plant growth regulators such as streptomycin (SM, 200 mg L
), mepiquat chloride (MC, 880 mg L
), and N-(2-chloro-4-pyridyl)-N'-phenylurea (CPPU, 2.5 mg L
) were treated to grapes. There were no noticeable differences in fruit growth and quality of grapes when only GA
were treated as 50 or 100 mg L
in all of triploid hybrid grapes. Statistically significant effects on the enlargement of cluster weight and berry size were shown in 'KB0120', 'KN200110', 'KS20015', 'KTS011', 'YRB9803' triploid hybrids treated with 100 mg L
2.5 mg L
CPPU. In combined treatment of 100 mg L
and 2.5 mg L
CPPU treatment, harvest time was delayed for up to 10 days. The sugar content and organic acid varied depending on the hybrid conditions. The decline of total sugar content was the highest in 100 mg L
200 mg L
SM for all hybrid grapes. There were no differences between 100 mg L
2.5 mg L
CPPU and GA
alone treatment in organic acid and total sugar contents. The results indicated that combined berry treatment of 100 mg L
and 2.5 mg L
CPPU seem to be useful for the production of high quality fruit in triploid hybrid grapes.
Effect of Waterlogging Conditions on the Growth, Root Activities and Nutrient Content of 'Campbell Early' Grapevine
Dhital, Shambhu Prasad ; Hwagn, Won-Nam ; Lim, Hak-Tae ;
Korean Journal of Horticultural Science and Technology, volume 28, issue 1, 2010, Pages 8~14
Effects of bromoethane (BE), ethanol (EtOH), and gibberellic acid (GA
) applications were evaluated for dormancy breaking of potato (Solanum tuberosum L.) tubers under room condition. Seed size (30-50 g/tuber) tubers of potato cvs. Gogu Valley, Purple Valley, Bora Valley and Superior were used. In the case of single application of chemicals, higher concentrations of BE and GA
resulted in earlier day of sprouting and earlier days to 80% sprouting; in contrast, higher concentrations of EtOH delayed the sprouting. Among the evaluated chemicals and concentrations, BE (0.2-0.3 mL
) showed the most desirable effect in breaking the tuber dormancy, which was about two times earlier than the untreated control. BE treated tubers also produced short and strong sprouts with the highest percentage of sprouted eyes in both of the tested potato cvs. Gogu Valley and Purple Valley. In the other experiment, double application of BE (0.2 mL
) at 24 h interval, and combined application of GA
) and BE (0.2 mL
) showed significant effect on breaking the tuber dormancy with high percentage of sprouted eyes and medium-length multi-sprouts; whereas, tubers treated with GA
alone produced long sprouts with few sprouted eyes in medium dormancy (Superior) and long dormancy (Bora Valley) potato cultivars. Tubers treated with BE (0.2 mL
) alone or combination of GA
) and BE (0.2 mL
) treatment showed the most number of main stems and higher tuber yield in the subsequent cultivation under field condition without any morphological changes in tuber and plant characters. Thus, the double application of BE (0.2 mL
) or combined application of GA
) and BE (0.2 mL
) is recommended for breaking dormancy of freshly harvested potato tubers in diverse potato cultivars.
Aging-related Changes of Inorganic Compound Leaching and Carbohydrates in Pyracantha angustifolia Seeds
Kim, Du-Hyun ; Han, Sim-Hee ;
Korean Journal of Horticultural Science and Technology, volume 28, issue 1, 2010, Pages 15~21
Changes of germination percentage, inorganic compound leaching, and carbohydrates were measured in Pyracantha angustifolia seeds throughout accelerated aging under 35
and 75% relative humidity (RH) to investigate whether these indicators can be used as a rapid vigor test. The decrease in seed germination was accompanied as increasing by Na
leakage concentration during artificial aging. However, the leachate electrical conductivity test and potassium and magnesium ion tests had no relation with seed germination. The ratios of inorganic ions from accelerated aged seeds show that K
, and Mg
were inversely correlated with germination and their correlation values were -0.75, -0.83, -0.79, and -0.79, respectively. Change in carbohydrates indicates that glucose concentration in aged seeds was associated with a decrease in seed vigor; however, the changes of sucrose and starch concentration did not show any relationship. In conclusion, the results show that, K
, and Mg
ratios and glucose concentration changes were highly correlated with seed vigor; thus, these changes could provide more sensitive and accurate index for the assessment of P. angustifolia seed vigor.
Cold Tolerance Assessment of Sedum Species for Shallow-Extensive Green Roof System
Kim, In-Hea ; Huh, Keun-Young ; Huh, Moo-Ryong ;
Korean Journal of Horticultural Science and Technology, volume 28, issue 1, 2010, Pages 22~30
This study was carried out to suggest an experimental base for selecting evergreen groundcover plants which can adapt well to the environmental condition of shallow-extensive green roof system by evaluating cold tolerance of seven Sedum species: Sedum acre, S. album, S. oryzifolium, S. reflexum, S. rupestre, S. sexangulare, and S. spurium. The cold tolerance of the plants subjected to laboratory low temperature treatments was evaluated using both electrolyte leakage and regrowth test, which was compared to electrolyte leakage responses of the plants exposed to on-site temperatures in a shallow-extensive green roof system. The lethal temperatures at the inflection points of sigmoidal curves fitted through electrolyte leakage were predicted with the range of -19.9
indicating that S. rupestre and S. sexangulare were more tolerant of low temperature while S. spurium and S. oryzifolium were less tolerant. These results were consistent with those from the regrowth test though the cold tolerance tested by electrolyte leakage evaluation tended to be rather overestimated. At the lowest on-site temperature recorded in December 2003 to January 2004 (-9.8
) electrolyte leakage values of the plants except S. oryzifolium were lower than 50% demonstrating that they could be applied stably to green roof system installed in South Korea during the winter.
Effects of Ringing Time on Vegetative Growth, Fruit Quality, and Return Bloom of 'Fuji'/M.9 Apple Trees
Sagong, Dong-Hoon ; Yoon, Tae-Myung ;
Korean Journal of Horticultural Science and Technology, volume 28, issue 1, 2010, Pages 31~35
In order to control tree vigor, 'Fuji'/M.9 apple trees in high density orchard were ringed width 5 mm in 2004 in tree trunk in different phenological stages; terminal bud break, full bloom and fruitlet (fruit size is about 15 mm). It was observed that ringing at bud break stage reduced the total shoot growth of trees to 64% of the uncut control, while ringing at full bloom stage to 73%, indicating that the early ringing, the more effective on controlling vegetative growth. When the trees were ringed at full bloom, return bloom was the highest, 85.7%, compared to the control of 33.6%. Ringing at bud break stage was remarkably increased return bloom to 56.0%, and resulted in low vegetative growth. Ringing at fruitlet stage only got a slightly higher return bloom compared to the control trees. Appropriate shoot growth reduction resulted from ringing in tree trunk before full bloom stage did not have certain negative effect on fruit quality and improve tree flowering in the following year. With respect to increasing return bloom and yield efficiency, the optimum ringing time was full bloom.
Effects of Bagging with Different Pear Paper Bags on the Color of Fruit Skin and Qualities in 'Manpungbae'
Kim, Yoon-Kyeong ; Kang, Sam-Seok ; Cho, Kwang-Sik ; Jeong, Sang-Bouk ;
Korean Journal of Horticultural Science and Technology, volume 28, issue 1, 2010, Pages 36~40
The study was conducted to examine physical characteristics of paper bags and evaluate skin color through in the fruits produced by bagging different paper bags. Newspaper bag showed the highest light transmittance with 6.6% and produced fruits which contain high chlorophyll contents in skin with 1.03 mg
showing that L and a values were good. Fruits skin by double or triple paper bags except for newspaper bag contained chlorophyll contents 0.86-0.7 mg
and they were increased as light transmittance rate increased. Especially, black outer bag and blue inner bag was similar to triple paper bags in light transmittance rate but hardness was similar to ones by newspaper bag. In result, it seemed that there was a close correlation among light transmittance of paper bag, chlorophyll content and appearance of the fruit skin in 'Manpungbae'.
Cultural and Pathogenic Characteristics Causing Anthracnose in Blueberry
Cheon, Mi-Geon ; Kwon, Jin-Hyeuk ; Jeong, Seon-Gi ; Kim, Min-Keun ; Chong, Byeong-Mahn ; Kang, Nam-Dae ; Shon, Gil-Man ; Park, Chang-Seuk ;
Korean Journal of Horticultural Science and Technology, volume 28, issue 1, 2010, Pages 41~45
The cultivation area of blueberry was magnified in Gyeongnam area and it was known as one of the most profitable crops in Korea. Breaking out in anthracnose during rainy season at the time of fruit maturity and ripeness causes a reduction in productivity and income. This experiment was conducted to minimize and pre-control the blueberry anthracnose by pesticides selection and infectiousness of it. To monitor pathogenicity in bluberry, leaf, stem and fruit were inoculated with several anthracnose strains which were the isolates in Gyeongsangnam-do Agricultural Research and Extension Services and Korea agricultural culture collection, 2 from red pepper and 1 from apple. Inoculation was done by needle and spray of anthracnose contained solution. Seven pesticides were treated on PDA medium to measure their inhibitory effect on the growth of anthracnose hypha. Anthracnose of blueberry fruit broke out much under the condition of high temperature and humidity, and appeared mainly in cracked region of ripened fruit skin. At 3-day after inoculation, disease lesion were visible on the leaf and fruit in the needle inoculation and on the fruit in the spray inoculation. It showed that 4 crops (red pepper, apple, grape, peach) inoculation of anthracnose that was grown in blueberry leaf was high in the spray inoculation in general, and an apple and a grape was produced pathogenic high needle inoculation. The effective fungicides for blueberry anthracnose control were Benomyl, Thiophanate-methyl and Tebuconazole.
Effect of Nutrient Solution Concentration on the Growth and Quality of Paprika Grown by Fertigation Using Waste Nutrient Solution
Zhang, Cheng-Hao ; Lim, Yong-Sup ; Kang, Ho-Min ; Kim, Il-Seop ;
Korean Journal of Horticultural Science and Technology, volume 28, issue 1, 2010, Pages 46~50
Waste nutrient solution (WNS) using non-recycled hydroponics for paprika (Capsicum annuum L.) decreased NH
-N, but increased Mg and SO
-S concentration as the paprika plants grew. EC and pH of WNS were maintained well at
15% levels of supplied solution, respectively. The paprika plants cultivated by WNS 2.0 dS
m-1 showed thicker stem diameter and higher leaf area index (LAI), but there were no significant differences in the plant height, number of leaf, and leaf thickness. The paprika plants cultivated by WNS showed higher chlorophyll content as they grew. The blossom end rot (BER) of fruits did not appear in WNS treatment, but the occurrence rate of BER was 5.3% in the fruits grown by pepper Hanbang fertigation solution (1.5 dS
, N: 9.4% (A)+11% (B), P: 6%, K: 28% (A)+22% (B), Mg: 3.5%, B: 0.1%, Zn: 0.003%, Cu: 0.0005%, Mo: 0.0005%). The total yield and number of fruit were higher in WNS 2.0 dS
treatment compared to the others. The paprika leaf grown by WNS had higher Mg and Ca contents. The K content of paprika leaf was higher in pepper Hanbang fertigation solution treatment. The fruit size of paprika classified by weight showed difference among treatments. The medium size fruits (100-150 g) were above 52% in WNS 2.0 dS
and Hanbang fertigation solution, whereas the percentage of small (<100 g) and large (>150 g) size fruits were the highest in Hanbang fertigation solution and WNS 2.0 dS
treatments, respectively. Therefore, for paprika plants cultivated in WNS 2.0 dS
, growth, yield and quality of fruit could be enhanced more than fertigation solution in fertigation culture.
Effects of Supplementary LED Lighting on Growth and Biochemical Parameters in Dieffenbachia amoena'Camella' and Ficus elastica 'Melany'
Heo, Jeong-Wook ; Lee, Yong-Beum ; Kim, Dong-Eok ; Chang, Yu-Seob ; Chun, Chang-Hoo ;
Korean Journal of Horticultural Science and Technology, volume 28, issue 1, 2010, Pages 51~58
To verify the possibility of LEDs as supplementary lighting sources for greenhouse production of foliage plants, growth and development, sugar content, starch content, and chlorophyll and carotenoid contents of potted Dieffenbachia amoena 'Camella' and Ficus elastica 'Melany' plants were investigated. The plant response varied by monochromatic or mixture of blue and red LEDs and by species. In both species, monochromatic red and supplementation with blue plus red LEDs increased plant height and number of unfolded leaves, while the blue plus red LED treatment promoted the uptake and utilization of nitrogen in Ficus plant. Supplementation with any LEDs did not cause any changes in contents of chlorophyll a and b and total carotenoids. Dry weight and starch content of Dieffenbachia and Ficus and the number of branches in Dieffenbachia increased by supplementation with mixture of blue and red LEDs without any negative effects compared with the control treatment. Results indicate that supplementation with mixture of blue and red LEDs, compared to other light treatments, is beneficial for promotion of growth and development without any negative effects on biochemical characteristics in Dieffenbachia and Ficus.
Analysis of Relationship among Growth, Environmental Factors and Transpiration in Soilless Culture of Paprika Plants
Tai, Nguyen Huy ; Park, Jong-Seok ; Ahn, Tae-In ; Lee, Jeong-Hyun ; Myoung, Dong-Ju ; Cho, Young-Yeol ; Son, Jung-Eek ;
Korean Journal of Horticultural Science and Technology, volume 28, issue 1, 2010, Pages 59~64
An accurate moisture control with precise estimation of transpiration of plants is required for low capacity and small size of growing substrate in soilless culture. In this study, a relationship among cumulative transpiration, radiation, vapor pressure deficit, and a relationship between cumulative irrigation and drainage with growth stage were analyzed for development of an optimum irrigation system. Paprika (Capsicum annuum L., cv. Cupura) plants were grown with a plant density of 4.05 plants/m
. For obtaining leaf area index (LAI), leaves were sampled every month. The LAI increased from 2.39 to 3.51 for 4 months, reflecting growth stage. Cumulative transpiration was calculated by subtracting the sum of cumulative drainage and an increase in slab weight from cumulative irrigation. Transpiration rate was changed responding proportionally to irradiation. By detailed analysis of transpiration and radiation, cumulative transpiration was regressed well with radiation integral by 2nd-order linear equations in different growth stages, while those with cumulative vapor pressure deficit was regressed by 1st-order linear equations. With increase of LAI, the slopes of cumulative irrigation and drainage curves and the difference between two slopes increased from 0.312 to 0.611, from 0.154 to 0.358, and from 0.158 to 0.253, respectively. In this study, we estimated cumulative transpiration of paprika with several environmental factors according to the plant growth stage and expect this data will contribute to making accurate irrigation strategy based on environmental and growing conditions.
Effect of Rootstock on the Tree Growth and Fruit Quality of 'Shiranuhi' Mandarin Hybrid in Plastic Film House
Moon, Young-Eel ; Kim, Chang-Myung ; Kim, Kwang-Sik ; Yun, Su-Hyun ; Park, Jae-Ho ; An, Hyun-Ju ; Lee, Dong-Hoon ; Moon, Doo-Khil ;
Korean Journal of Horticultural Science and Technology, volume 28, issue 1, 2010, Pages 65~69
The tree growth and fruit quality of 'Shiranuhi' [(Citrus unshiu
C. reticulata] grafted on five rootstocks were measured in 2004 to 2006 namely, 'Trifoliate orange' (Poncirus trifoliata), 'Swingle citrumelo' (C. paradisi
P. trifoliata), 'Shiikuwasha' (C. depressa Hayata), 'Yuzu' (C. junos Sieb.), and 'Shin Amanatsu' (C. natsudaidai) in plastic film house. The trunk diameter, spring shoot length, and the height and width of the tree were significantly higher in 'Swingle citrumelo' and 'Shiikuwasha' than the other rootstocks in 2004 and 2006. The fruit widths were not significantly different in five rootstocks. The fruit height of 'Swingle citrumelo' was the highest compared with other rootstocks. The number of fruits and the yield per tree in 'Swingle citrumelo' were significantly the highest among the rootstocks studied in 2004 and 2006. Soluble solids content and acidity of 'Swingle citrumelo' and 'Shiikuwasha' were significantly lower than those of 'Trifoliate orange'. The concentration of glucose in 'Swingle citrumelo' and 'Shiikuwasha' rootstock was lower than those of other rootstocks, but the concentration of sucrose in 'Swingle citrumelo' increased, which was higher than other rootstocks. In 'Swingle citrumelo', concentration of citric acid was lower and malic acid was higher than 'Trifoliate orange'.
Quality Changes of King Oyster Mushroom as Influenced by Controlled Atmosphere Regimens during Storage and Shelf Temperature Conditions
Park, Youn-Moon ; Jhune, Youn-Moon ;
Korean Journal of Horticultural Science and Technology, volume 28, issue 1, 2010, Pages 70~76
Changes in quality of king oyster mushrooms (Pleurotus eryngii) were investigated under controlled atmosphere (CA) storage and simulated marketing conditions to provide basic ideas for modified atmosphere packaging (MAP) application during export process. Mushrooms were harvested on Nov. 9, precooled to 4
within 4 h, and then stored under two CA regimens with different CO
conditions; 5 kPa O
+ 10 kPa CO
and 5 kPa O
+ 15 kPa CO
. Storage temperature was maintained at 0.5
with 95% RH. After 3 and 4 week storage, mushrooms were removed from the CA, packaged into polypropylene container, and then put on the shelf for 5 and 7 days at 20
, respectively. Beneficial effect of 15 kPa CO
CA was very limited on the reduction of stem browning ratings, which was significant only at 5 days on the shelf after 4-week storage. High CO
CA storage tended to reduce ethanol accumulation inside packages during 20
shelf life, with no effect on off-flavor rating. In contrast, low shelf temperature significantly maintained better sensory and physicochemical quality attributes, and overall marketability. The results suggest different potentials for shipping plus marketing period by shelf temperature regardless of high CO
-CA conditions; 3-week shipping + 5-day marketing period when 20
shelf temperature is unavoidable, whereas, under 7
marketing conditions, the potential period could be extended to > 4-week shipping + 7-day marketing period.
Changes in Residual Chlorine Content on Fresh-cut Iceberg Lettuce after Chlorine Treatment
Cho, Sun-Duk ; Chang, Min-Sun ; Lee, Yu-Si ; Ha, Sang-Do ; Kim, Gun-Hee ;
Korean Journal of Horticultural Science and Technology, volume 28, issue 1, 2010, Pages 77~81
This study analyzes the residual chlorine content of fresh-cut lettuce (Lactuca sativa L.) according to changes the concentration of sodium hypochlorite, dipping time, number of washes, and the temperature of wash water to verify the level of toxicity. When the concentration of sodium hypochlorite was under 200 mg
, the residual amount of free chlorine was 1.0-7.0 mg
. However, when the concentration of sodium hypochlorite exceeded 300 mg
, the residual amount dramatically increased to 30.0-45.0 mg
. The amount of free chlorine did not change much by the time of dipping. In contrast, the total residual chlorine content rose slightly as the dipping time increased. When the subject was washed once after chlorine treatment, the total residual chlorine content stood at 16.0 mg
. Residual chlorine was not detected when the subject was washed for more than two times. Between the chlorinated water (200 mg
) temperature of 5-20
, the total residual chlorine content was 22.0-28.0 mg
. When the temperature of washing water was raised to 30-50
, the content decreased to 5.0-12.0 mg
Effects of Ethylene Absorbent on Quality Changes of Fresh Ginger Rhizomes during Modified Atmosphere Storage
Chung, Hun-Sik ; Lee, Hyeon-Jeong ; Moon, Kwnag-Deog ;
Korean Journal of Horticultural Science and Technology, volume 28, issue 1, 2010, Pages 82~88
Fresh ginger rhizomes were kept in sealed low density polyethylene (0.04 mm thickness) bags with and without an ethylene absorbent for 5 months at 12
. The effects of ethylene removal on the changes in gas levels of the packages and quality characteristics of the rhizomes were studied. Oxygen and carbon dioxide levels in the packages with ethylene absorbent were maintained higher and lower, respectively, compared to control. Decrease of L
value and increase of a
value in surface and internal tissue of the rhizomes were found to be higher in the rhizomes packaged with than without ethylene absorbent. Sprouting and rotting were restrained by ethylene absorbent treatment. Weight loss, flesh firmness, soluble solids content and pH were not affected by ethylene absorbent. Sensory score of appearance, odor and overall acceptability were higher in ginger packaged with than without ethylene absorbent. These results show that ethylene absorbent could be used for retarding the sprouting and rotting and for retention of the sensory quality of fresh ginger rhizomes.
Evaluation of TYLCV-resistant Tomato Germplasm Using Molecular Markers
Park, Young-Hoon ; Kim, Kwang-Hwan ; Choi, Young-Mi ; Choi, Hak-Soon ; Chae, Young ; Park, Kwon-Seo ; Chung, Sang-Min ;
Korean Journal of Horticultural Science and Technology, volume 28, issue 1, 2010, Pages 89~97
Tomato yellow leaf curl virus (TYLCV) infection causes serious economic losses in tomato production world-wide, and the development of new domestic cultivars with high levels of resistance to TYLCV is required. In this study, major advanced breeding lines carrying TYLCV-resistance loci, Ty-1, Ty-2, Ty-3, and Ty-3a were collected from overseas breeding programs, and their allelic composition of each resistance locus were confirmed by using public PCR-based DNA markers. Perfect matches between marker genotypes and the resistance indicated a feasibility of marker-assisted selection (MAS). In addition, genetic relationship of the TYLCV-resistant germplasm with commercial hybrids broadly grown in South Korea and other breeding lines were evaluated by AFLPs. A very close genetic background among these tomato germplasm revealed by the genetic similarity coefficient (0.87 to 1.00) and UPGMA implied the easiness of a rapid incorporation of the resistance genes. Phenetic tree showed a close relationship between TYLCV-resistant CLN lines and general type commercial hybrids, while Gc lines formed an independent group distinguished from both general and cherry type cultivars. Pyramiding TYLCV-resistance alleles using the resistant germplasm and DNA markers introduced in this report will greatly improve the breeding process for high levels of TYLCV-resistance. For accurate evaluation of the TYLCV-resistance, however, a reliable method of bio-assay still remains to be established.
Analysis of Ploidy and S-alleles of 'Kiyo' a Japanese Plum
Jun, Ji-Hae ; Kwon, Jung-Hyun ; Do, Kyeong-Ran ; Yun, Ik-Koo ; Ma, Kyung-Bok ; Kim, Jung-Bae ;
Korean Journal of Horticultural Science and Technology, volume 28, issue 1, 2010, Pages 98~102
'Kiyo' was newly planted in many plum orchards in Korea and Japan. However, farmers who planted 'Kiyo' have suffered from little fruit set. To elucidate the cause for bad fruit set of 'Kiyo', we investigated S-alleles of S-RNase which is based on self-incompatibility of Japanese plum (Prunus salicina Lindl.). The compared eight cultivars of Japanese plum had 2 alleles while 'Kiyo' had 3 alleles, Sc, Sd and Sf. Most of Japanese plums are diploids (2n=2x=16) but 'Kiyo' was found to be a triploid (2n=3x=24) by cytometric analysis. A triploid 'Kiyo' produced more poor and abnormal pollens than 'Simka', a diploid Japanese plum. 'Kiyo' could set the fruits with pollens of 'Biocherry', 'Hollywood', and 'Simka' by artificial pollination.
Early Detection of Cymbidium Mosaic Virus and Odontoglossum Ringspot Virus in Tissue-Cultured Phalaenopsis Plantlets
Choi, Sun-Hee ; Hong, Jin-Sung ; Ryu, Ki-Hyun ;
Korean Journal of Horticultural Science and Technology, volume 28, issue 1, 2010, Pages 103~107
Virus resistant Phalaenopsis breeding requires a lengthy time to make cultivar with qualification for obtaining practical virus resistance. We analyzed infection pattern and possibility for early detection of both CymMV and ORSV in tissue cultured Phalaenopsis plantlets to shorten the breeding time to selection of virus resistant Phalaenopsis. Both viruses could be detected by RT-PCR with specific primers for coat protein (CP) and by western blotting with respective CP specific antiserum in tissue cultured Phalaenopsis plantlets inoculated deliberately at the early stage (1-4 dpi) and we could monitor virus movement pattern from the site of initial inoculation to other parts of the leaf tissue by serial observation according to dpi. This study provides methods to confirm true virus resistance by mechanical inoculation for transgenic orchids against virus infection, and can be adopted to raise selection efficiency. Therefore, it might be useful to shorten the breeding period with rapid detection of virus-resistant transgenic plantlets in vitro or check for virus free in tissue-cultured Phalaenopsis.
Factors Affecting on in Vitro Gametophyte Formation from Spore Culture of Four Moss Species
Ahmed, Giush Uddin ; Chang, Young-Deug ; Lee, Cheol-Hee ;
Korean Journal of Horticultural Science and Technology, volume 28, issue 1, 2010, Pages 108~114
Effects of various culture media and physical environments on in vitro spore germination and bud inductions of four mosses were studied. Primary cultures were initiated from spores of Hypnum plumaeforme, Amblystegium serpens, Bartramia pomiformis and Polytrichum commune. Spores were cultured on hormone-free Knop and three concentrations of MS (1/2 MS, 1/4 MS, 1/8 MS) media, and low concentration of mineral salts (1/8 MS) stimulated spore germination and growth of protonema. Protonemata were then subcultured on the same media, and intermediate strength (1/4 MS) of the culture medium was required for optimal bud induction as well as for vegetative growth. Bud free protonemata were tested at four temperatures (15, 20, 25 and 30
) under 27
illumination. All species exhibited maximum gametophyte numbers and height at 20 and 25
. Cultures were also subjected to different light intensities: 13.5, 27, 40.5 and 54
at the optimal temperature (20
). Light intensity within the range of 27 to 40.5
was optimum for bud induction. So, it was confirmed that culture media and environmental factors regulated protonema differentiation and bud induction of mosses.
Antioxidant Activities of Fractions Obtained from Flowers of Coreopsis tinctoria Nutt.
Woo, Jeong-Hyang ; Jeong, Heon-Sang ; Chang, Young-Deug ; Shin, So-Lim ; Lee, Cheol-Hee ;
Korean Journal of Horticultural Science and Technology, volume 28, issue 1, 2010, Pages 115~119
The objective of this study was to examine the possibility of using Coreopsis tinctoria flowers as a new natural antioxidant. Flowers of C. tinctoria were solvent fractioned stepwise with n-hexane, chloroform, ethyl acetate, n-butanol and water. In n-butanol fraction, soluble solid yield was the highest. Ethyl acetate fraction showed the highest contents of total polyphenol and total flavonoid whose RC
, respectively. The most effective DPPH and ABTS radical scavenging activities were shown in ethyl acetate fraction whose RC
, being even more effective than BHT and similar to ascorbic acid. Next was n-butanol with high DPPH and ABTS radical scavenging activities whose RC
, respectively. In conclusion, it was confirmed that flowers of C. tinctoria have high antioxidant effect and thus have high potential as good sources of natural antioxidants.
Antioxidant Activity and Quinone Reductase Inductive Activity of Pepper Leaves according to Leaf Lengths
Ku, Kang-Mo ; Kim, Byung-Su ; Kang, Young-Hwa ;
Korean Journal of Horticultural Science and Technology, volume 28, issue 1, 2010, Pages 120~125
Various leaf lengths of pepper (Capsicum annuum L.) leaves were examined to evaluate the antioxidant activity and quinone reductase (QR) inductive activity. Total phenolic (TP) and total flavonoid (TF) contents were decreased as leaf length was increased. Antioxidant activity was also shown to be decreased as leaf length was increased. There were significant correlations between antioxidant activities and TP and TF. Thus, it could be assumed that phenolic and flavonoid compounds were responsible for antioxidant activity of pepper leaves. On the other hand, QR inductive activity and total chlorophyll content were increased as the leaf length was increased. This study revealed that total phenol and total flavonoid content are significantly different depending on length of pepper leaves. As a result, antioxidant activity and QR inductive activity of pepper leaves are dependent on length of pepper leaves.
Application of Chlorophyll Fluorescence as a Useful Tool for Evaluating the Influence of a Gamma Ray on Some Horticultural Crops
Kim, Jing-Hong ; Moon, Yu-Ran ; Lee, Min-Hee ; Kim, Tae-Hoon ; Lee, Ju-Woon ; Chung, Byung-Yeoup ;
Korean Journal of Horticultural Science and Technology, volume 28, issue 1, 2010, Pages 126~131
With the data reported in this paper, we demonstrate how the chlorophyll fluorescence analysis can be utilized in evaluating the influence of gamma rays on plants. When seedlings of five horticultural vegetables, Chinese cabbage, cucumber, tomato, lettuce, and red pepper, were irradiated with an acute dose of 50 Gy
for 4 h, they didn't show noticeable changes in the phenotype and the maximum photochemical efficiency of photosystem II, Fv/Fm. However, the performance index (PI) of photosynthesis which is based on the OJIP induction kinetics of chlorophyll fluorescence, was decreased in the irradiated leaves of all the plant species tested, especially in those of tomato and red pepper. Similarly, the buildup of the non-photochemical quenching (NPQ) was suppressed by the gamma irradiation. The marked inhibition of the NPQ in the irradiated leaves of cucumber, tomato, and pepper was easily distinguishable in the respective chlorophyll fluorescence images. These data suggest that the PI and NPQ are radiation-sensitive biological parameters applicable to the plant ecological monitoring of natural or artificial radiation environments.
Effect of Horticultural Therapy Program Applying Prompt on the Hand Function of the Intellectual Disabilities
Lee, Min-Jung ; Kim, Hong-Yul ;
Korean Journal of Horticultural Science and Technology, volume 28, issue 1, 2010, Pages 132~137
This study was carried out to determine the effect of horticultural therapy program applying prompt on the hand function of the intellectual disabilities. From January 2007 to November 2008, flower pressing and flower decoration were carried 24 times every week while planting and topiary were performed 20 times at week intervals. These programs were intended to improve the fingertip power, grip power, writs power of the intellectual disabilities through the horticultural therapy which utilized prompt. As for the fingertip power, the flower pressing program resulted in significantly improved power in the left hand (P=0.009) and right hand (P=0.001). As for the grip power, the flower decoration brought about the right hand (P=0.019). In the case of the wrist power, the flower pressing resulted in a significasignificant improvement in the right hand (P=0.020), and the topiary resulted in a significant improvement in nt improvement in the left hand (P=0.041), while the planting resulted in a significant improvement in the right hand (P=0.028). Meanwhile, the topiary brought about a significant improvement in the right hand (P=0.019) and left hand (P=0.003). The evaluation results on the hand function indicated that topiary was the program that helped improve overall hand functions. It was found that the flower pressing was highly effective in increasing the power of fingertip, and the flower decoration was very effective in boosting the grip power. Meanwhile, planting was very effective in building the wrist power. Based on the aforesaid results, it can be found that horticultural programs using prompt have different effects on the improvement of hand functions such as the power of finger tip, grip power, and wrist function.
Aroma Components and Antioxidant Activities of Pure Lavender Essential Oil Goods in Different Produced Countries
Woo, Jin-Ho ; Mok, Min-Gyun ; Park, Kuen-Woo ;
Korean Journal of Horticultural Science and Technology, volume 28, issue 1, 2010, Pages 138~143
The internal and external circulation of pure (100%) lavender (Lavandula angustifolia Mill.) essential oils (EO) were collected from 33 kinds produced different countries. These samples were analyzed of its aroma components and measured of the antioxidant activity. As the result of analyzing the components of lavender EO including linalool, linalyl acetate, pinene, camphene, camphor, and borneol were identified. Main components (linalool, linalyl acetate) content difference of lavender essential oil appeared by Australia> France> Not marked origin> etc..> South Korea. However, all samples weren't met requirement of ISO lavender standard. Electron donating ability (EDA) in the 2.6 to 94.0% contents showed significant differences. EO produced in Australia, France, Switzerland, Germany, and Korea was more than 50% of antioxidant activity. Most of EOs that have high contents of linalool and linalyl acetate showed high antioxidant effect. By comparing antioxidant activity according to the listed place of origin, it showed highest antioxidant activity from the EO produced in Australia. The high antioxidant activity of 52.1% of EO cultivated and extracted in Korea, despite the low content of linalool and linalyl acetate content of EDA (%), could be considered that the
-phellandrene, which is the monoterpene component is contained in large composition.
Effects of a Horticultural Program on the Preschoo Children's Emotional Intelligence and Daily Stress
Park, Seong-Hyun ; Huh, Moo-Ryong ;
Korean Journal of Horticultural Science and Technology, volume 28, issue 1, 2010, Pages 144~149
Preschool is currently underutilized as a potential setting to enhance emotional intelligence and stress reduction in children. This study investigated the effects of a horticultural program on the preschool children's emotional intelligence and daily stress. Sixty children with ages 5 to 7 participated in this study. Children in the experimental group participated in a one-hour horticultural program twice a week for four months. An activity room and an outside garden in the preschool were used for the horticultural program. Data collected included the Emotional Intelligence Rating Scale (EIRS), the Korean Preschool Daily Stress Scale (KPDSS), the Horticultural Program Evaluation (HPE) and demographic measures. The program made a statistically significant impact on differences in the EIRS scores between groups (P=0.001). Among the EIRS subcategories, "appraising and expressing emotions in self" and "regulating emotions in others and handling relationships" significantly increased over the course of the program for the experimental group. The KPDSS was significantly different between groups as well (P=0.01). Among the KPDSS subcategories, significant increase was found in "hurt pride" for the experimental group. Positive outcomes were also found in the HPE for the experimental group indicated by high preference of the program. In conclusion, the horticultural program was effective for preschool children in improving emotional intelligence and in decreasing daily stress. Further investigations should examine aspects of gardening that have the greatest impact on influencing emotional intelligence and stress reduction.
Effects of the Horticultural Activity Program on Change of Intergenerational Perception and Interaction of the Elderly and Young Children
Lee, Eun-Sook ; Pak, Chun-Ho ;
Korean Journal of Horticultural Science and Technology, volume 28, issue 1, 2010, Pages 150~154
This study investigated the effects of the horticultural activity program on the change of intergenerational perception and interaction for the elderly and young children. The result of repeated measurements of the behavior of young children in interactions between the elderly and young children for horticultural activity program, positive interaction increased while negative interaction decreased. When a comparison was made before, during, and after treatment, the control group didn't show a significant difference in the perception of the elderly; on the contrary, treatment group showed a highly significant difference. In the perception of young children, the control group and the treatment group didn’t show a significant difference. The results suggest that horticultural activity program can help young children to improve perception of the elderly by young children through positive interaction.
A New Gerbera 'Sugar Pink' with Semi-double for Cut Flower
Chung, Yong-Mo ; Hwang, Ju-Chean ; Chin, Young-Don ; Kim, Su-Kyeong ; Roh, Chi-Woong ; Yi, Young-Byung ; Kwon, Oh-Chang ;
Korean Journal of Horticultural Science and Technology, volume 28, issue 1, 2010, Pages 155~158
A new pink gerbera (Gerbera hybrida Hort.) 'Sugar Pink' was developed from a cross 'Temptation' and 'Grandeur' followed by seedling and line selections at the Flower Research Institute, Gyeongnam Agricultural Research and Extension Services (ARES) in 2006. Characteristic tests were conducted three times from 2004 to 2006. 'Sugar Pink' has semi-double type in good harmony with bright pink (RHS 68-C) ray floret and green center. It has good stable flower shape and strong peduncle. Its vase life was 13.8 days. The average yield of 'Sugar Pink' was 49.2 flowers per plant a year in greenhouse yield trials carried out from 2004 to 2006. This cultivar was registered for commercialization in 2006. 'Sugar Pink' could be cultured under greenhouse condition in Korea.
Simultaneous Blooming 'Shine', a New Cultivar of Gypsophila paniculata
Cheong, Dong-Chun ; Lim, Hoi-Chun ; Choi, Chang-Hak ; Kim, Kab-Cheol ; Song, Young-Ju ; Kim, Jeong-Man ;
Korean Journal of Horticultural Science and Technology, volume 28, issue 1, 2010, Pages 159~161
'Shine', a cultivar of Gypsophila paniculata was newly developed for cut flowers by Floriculture Resource Research Institute of Jeonbuk Agricultural Research and Extention Services. It was selected by selection of seedlings and lines in a seed-set bud mutant (Gyp99 line) derived from 'Bristol Fairy' in 1999. Its several traits were evaluated from 2005 to 2006. Its plant type is the upright type, and the flower characteristic is many semi-double florets with emarginate petal tip and campanulate calyx shape. 'Shine' had a trait of simultaneous blooming of many florets and moreover it produced normal flowers with two pistils, with little production of malformed flowers in high temperature growth conditions. It also differentiated more node and primary branches number, and longer cut flower stalk, larger leaf length and calyx size, whereas there was less petal number and narrower branching angle than check variety 'Million Stars'. The blooming of 'Shine' in summer cultivation was delayed by 17 days, compared to 'Million Stars'.
A New Cymbidium 'Yellow Princess' with Bright Yellow Flower Color and Medium Sized Plant
Kim, Mi-Seon ; Cho, Hae-Ryong ; Lee, Hye-Kyung ; Lim, Jin-Hee ; Shin, Hak-Ki ;
Korean Journal of Horticultural Science and Technology, volume 28, issue 1, 2010, Pages 162~165
A new Cymbidium variety 'Yellow Princess' was developed from a cross between 'Eastern Star' and 'Tropical Yellow' followed by seedling and line selections at the National Institute of Horticultural and Herbal Science (NIHHS), Rural Development Administration (RDA) in 2006. Trials were conducted from 2000 to 2005 for evaluation and selection of this cultivar. 'Yellow Princess' has medium flowering habit and bright yellow flowers (RHS, Y11B: petal and sepal) with grayed purple (GP183A) spot on lip when fully opened. The number of flower on each peduncle was 12.9 and the flower diameter was 6.8 cm. It has adequate peduncle height (56.5 cm) compared to leaf length (75.0 cm). The general impression of petals and sepals show some incurved and some spreading shape. It blooms starting from early-winter (from Nov.) under optimal culture conditions.