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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Horticultural Science and Technology
Journal Basic Information
Journal DOI :
Korean Society of Horticultural Science
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Volume & Issues
Volume 33, Issue 5 - Oct 2015
Volume 33, Issue 4 - Aug 2015
Volume 33, Issue 3 - Jun 2015
Volume 33, Issue 2 - Apr 2015
Volume 33, Issue 1 - Feb 2015
Selecting the target year
Effects of Elevated Spring Temperatures on the Growth and Fruit Quality of the Mandarin Hybrid 'Shiranuhi'
Moon, Young-Eel ; Kang, Seok-Beom ; Han, Seung-Gab ; Kim, Yong-Ho ; Choi, Young-Hun ; Koh, Seok Chan ; Oh, Soonja ;
Korean Journal of Horticultural Science and Technology, volume 33, issue 4, 2015, Pages 459~469
DOI : 10.7235/hort.2015.14190
The effects of elevated spring temperatures on the growth and fruit quality of the mandarin hybrid 'Shiranuhi' [(Citrus unshiu
C. reticulata] were investigated in plastic greenhouses, to develop a cropping system to improve the quality of the fruit and increase the income of growers on Jeju Island, South Korea. Under conditions of elevated temperature I (
, day/night) and elevated temperature II (
, day/night) during early spring, budburst was advanced by 11 and 15 d, and full bloom by 22 and 45 d, respectively, compared to those of the plants grown at ambient air temperature in a plastic greenhouse. Elevated temperatures decreased the number of spring shoots but increased mean spring shoot length and leaf area. Growing 'Shiranuhi' trees at elevated temperatures resulted in increases in mean fruit weight and fruit L/D ratio (> 1.0). In addition, fruit color development was significantly advanced in trees grown under elevated temperatures during early spring, which allowed the fruit to be harvested 1-2 months earlier than trees grown under ambient air temperature. Fruit soluble solids content (SSC) and titratable acidity (TA) at harvest were similar between elevated temperature I and ambient air temperature, but were significantly higher than at elevated temperature II. Considering fruit quality, harvest time, and yield, the elevated temperature treatment regime of
(day/night) during early spring could be useful for cultivation of the mandarin hybrid 'Shiranuhi' to increase the income of growers.
Anthocyanin Composition and Characteristics of 'Gaeryangmeoru', 'Kyoho', and 'Hongisul' Grape Varieties
Kwon, YongHee ; Park, Hee-Seung ;
Korean Journal of Horticultural Science and Technology, volume 33, issue 4, 2015, Pages 470~478
DOI : 10.7235/hort.2015.15029
This study was investigated the anthocyanin composition of 'Gaeryangmeoru', 'Kyoho', and 'Hongisul' grapes cultivated in Korea using ultra-performance liquid chromatography (UPLC) coupled to a mass spectrometer (MS) equipped with an ESI (electrospray ionization) source. 'Gaeryangmeoru' is a dark-blue grape used for winemaking that can reach its coloring in unfavorable weather. The 'Kyoho' and 'Hongisul' varieties are hybrid grapes that feature black and pink skin, respectively. The anthocyanins extracted from the peels of grapes were analyzed using UPLC-ESI-MS/MS. Twenty-five anthocyanins were identified in the 'Gaeryangmeoru' and 'Kyoho' varieties, and 21 were identified in the 'Hongisul' variety. Eight, 14 and five predominant anthocyanins were identified in 'Gaeryangmeoru', 'Kyoho' and 'Hongisul' grape respectively. In all three varieties, mono-glucosides were 2.3-5.9 times more abundant than di-glucoside. Malvidin was the predominant anthocyanidin in 'Gaeryangmeoru' (44.1%) and 'Kyoho' (56.5%), but cyanidin (96.8%) was in 'Hongisul'. The acylated anthocyanins in 'Gaeryangmeoru' (2.0%) were rare, whereas acylated anthocyanins with p-coumaric acid were predominant in 'Kyoho' (40.9%) and 'Hongisul' (70.7%). In particular, cyanidin feruloyl glucoside was found only in the 'Hongisul' cultivar and considered to be useful as a criterion for identification of the variety. As a result, the grape varieties were demonstrated to have variety-specific anthocyanin characteristics, enabling classification based on anthocyanin composition in terms of anthocyanidins, glucosylation and acylation. The taxonomical application of anthocyanin composition confirmed the possibility that 'Gaeryangmeoru' originated from Vitis amurensis or its hybrids, and the 'Hongisul' grape was distinguished from other grapes by cyanidin feruloyl glucoside.
Occurrence of Micro-cracking According to Bagging Paper in 'Mansoo' Pear Fruits
Choi, Jin-Ho ; Yim, Sun-Hee ; Kim, Sung-Jong ; Lee, Han-Chan ; Kwon, YongHee ;
Korean Journal of Horticultural Science and Technology, volume 33, issue 4, 2015, Pages 479~485
DOI : 10.7235/hort.2015.15026
This study was conducted to study the factors inducing micro-cracking and determine proper practices to reduce its occurrence in 'Mansoo' pear fruits. Micro-cracking was observed on ripe fruits. Occurrence of micro-cracking was closely related to sun duration time in August. Micro-cracking occurred severely with shorter sun duration, but weakly with longer sun duration and continuous sunlight. Micro-cracking fruits occurred more frequently in trees with a pergola training system than in those with Y-trellis, but there was no difference between the irrigated and non-irrigated groups. While no micro-cracking was observed without fruit bagging, micro-cracking occurred in fruits with black and yellow outer paper bagging at rates of 62.2 and 17.3%, respectively. Our results suggest that the light condition was the most important cause of micro-cracking because the occurrence of micro-cracking in 'Mansoo' fruits was affected by factors including sun duration, trellis system and fruit bag color. We suggested that micro-cracking could be reduced in 'Mansoo' fruit by optimizing the light conditions with the proper trellis system, bagging color and summer pruning.
Comparison of Fruit Development and Quality Indices According to Blossom Thinning on Early-season 'Hanareum' and Mid-season 'Niitaka' Pears
Lee, Ug-Yong ; Kim, Yoon-Kyoung ; Shin, Il-Sheob ; Oh, Kwang-Suk ; Jung, Ok-Kun ; Chun, Jong-Pil ;
Korean Journal of Horticultural Science and Technology, volume 33, issue 4, 2015, Pages 486~491
DOI : 10.7235/hort.2015.15030
In this study, we evaluated the differences in fruit development and quality indices of fruits subjected to blossom thinning before full bloom in 'Hanareum' and 'Niitaka' pears (Pyrus pyrifolia Nakai). We carried out a thinning treatments at 5 days before full bloom, targeting the blossoms at the 1st and 2nd position (T1) or the blossoms at the 6th and 7th position (T2) from the basal part of the flower cluster in both cultivars. Blossom thinning treatments increased the average fruit weight of early-season Asian pear 'Hanareum' up to 10.5% (T1) and 11.0% (T2) at harvest time (110 days after full bloom). The mid-season 'Niitaka' pear showed increased fruit weight of up to 12.6% (T1) and 16.8% (T2) at harvest time (170 days after full bloom). Moreover, these treatments increased the production rate of bigger sized fruits in both cultivars. Removal of blooms also affected fruit quality indices; the bloom-thinned fruits showed higher soluble solids contents (about
) and higher skin color redness (
) especially in 'Niitaka' pears when compared to the non-thinned controls. Overall, the treatments increased the fruit size and weight as well as fruit quality including soluble solids and skin color. Bloom thinning treatment targeting the 6th to 7th blossom from the basal part of the flower cluster may be recommended as the practical thinning method in Asian pear 'Hanareum' and 'Niitaka'.
Antagonistic Mechanisms and Culture Conditions of Isolated Microbes Applied for Controlling Large Patch Disease in Zoysiagrass
Kim, Young-Sun ; Ma, Ki-Yoon ; Lee, Geung-Joo ;
Korean Journal of Horticultural Science and Technology, volume 33, issue 4, 2015, Pages 492~500
DOI : 10.7235/hort.2015.15066
Our previous report demonstrated successful isolation of soil-borne bacteria that suppressed the potential of Rhizoctonia solani AG2-2 (IV) causing turfgrass large patch disease when applied to Korean lawngrass (Zoysia japonica). The current study aimed to uncover the mechanisms of this antagonism of Rhizoctonia solani and to define culture conditions for the isolated microbes. We found that two Bacillus isolates, I-009 and FRIN-001-1 strains, produced cellulase and siderophore, but not chitinase, while the Pseudomonas YPIN-022 strain was found to release only siderophore, implying that three antagonistic bacteria commonly interrupt Fe uptake by the large patch pathogen. The I-009 and FRIN-001-1 isolates grew best at 35 and
in growth medium of pH 5 to 8 for 32 and 28 h, respectively, while optimum growth for the YPIN-022 strain was found at
at pH 5 to 9 for 24 h. Good growth of I-009 and YPIN-022 over 24 h was obtained in M9 minimal medium supplemented with 1% sucrose, 0.5% yeast extract and 0.1% potassium chloride. FRIN-001-1 grew well in M9 medium with 1% mannitol, 0.5% yeast extract and 0.1% potassium phosphate dibasic.
Changes in Dormant Phase and Bud Development of 'Fuji' Apple Trees in the Chungju Area of Korea
Lee, ByulHaNa ; Park, YoSup ; Park, Hee-Seung ;
Korean Journal of Horticultural Science and Technology, volume 33, issue 4, 2015, Pages 501~510
DOI : 10.7235/hort.2015.15010
In this study, we investigated the onset and release of endo-dormancy under natural conditions by observing bud break characteristics in 'Fuji' apple trees using water cuttings. Through examinations of bud break rate and days to bud break, we found that the endo-dormancy of 'Fuji' apple tree continues for 70 d from 165 to 255 d after full bloom (DAFB), from late October to early January of the following year. In addition, within 20 d of first bud break, based on a final bud break rate of 60% or more, we able to identify the timing of the changeover from para-dormancy to endo-dormancy, and endo-dormancy to eco-dormancy. Analysis of the chilling requirement during the endo-dormancy period revealed that chilling accumulation up to 255 DAFB to release endo-dormancy amounted to 666 and 517 h based on the CH and Utah models, respectively. Observation of internal changes in the bud during endo-dormancy showed that flower bud differentiation begins from mid-July, and t ime of inflorescence o f the disk f lower is a vailable to f ind. The f lower buds subsequently developed slowly but steadily during endo-dormancy and in the following year in February, the developmental stage of each organ had progressed. Moreover, the flower buds of 'Fuji' apples were mostly healthy during the dormancy period, but some exhibited necrosis of flower primordium, due partial cell damage from the formation of ice crystals rather than a direct effect of the low temperature. Flower buds were formed in both the axillary buds of bourse shoots and terminal buds of spurs, but lower bud differentiation was observed for the terminal buds of spurs at rate of about 65% of total buds, which was directly related to the bud size and shoot diameter.
Influence of Root Restriction Materials and Media on Soil Environment and Growth of Runner Plantlets during Propagation of 'Seolhyang' Strawberry
Park, Gab Soon ; Chae, Soo Cheon ; Oh, Chan Sik ; Choi, Jong Myung ;
Korean Journal of Horticultural Science and Technology, volume 33, issue 4, 2015, Pages 511~517
DOI : 10.7235/hort.2015.15003
This research was conducted to evaluate the influence of root restriction materials and media on the growth of runner plantlets of 'Seolhyang' strawberry in a nursery field. To achieve this, the influence of three kinds of root media on the growth of runner plantlets was monitored when polyethylene film was used as the root restriction material. In addition, the influence of various root restriction materials (RRS) such as transparent polyethylene film (PE), non-woven fabric (NF), perforated polyethylene film (PP), and root proofing sheet (RPS) on the changes in volumetric water content (VWC) and temperature of root media as well as growth of runner plantlet were investigated when expanded rice hull (ERH) was used as the root medium. In the comparison of root media, growth parameters such as leaf area and crown thickness at 20 d after fixation as well as crown thickness and fresh weights of root and above-ground tissue at 40 d after runner plantlet fixation were higher in the ERH treatment than in sandy loam and loamy sand. When the influence of RRS was compared, the VWC of ERH was 55% just after irrigation, but decreased to 26% at just before irrigation. Ranges of the VWC as influenced by irrigation cycle were 16 to 10% in the PP and less than 10% in the NF and RPS. The soil temperature in the PE treatment was around
lower than in NF, PP, and RPS. The differences between day and night temperatures were also smaller in the PE treatment rather than those in NF, PP, and RPS. The growths of runner plantlet 50 d after fixation showed that plant heights as well as fresh weights of root and above-ground tissue were higher in the PE treatment than in NF, PP, and RPS. NF and PP did not effectively restrict roots inside the medium and the roots of runner plantlets penetrated through the root restriction materials resulting in the formation of root system below the restriction materials. The above results indicate that ERH is more effective than sandy loam or loamy sand as root medium. PE rather than NF, PP, or RPS as root restriction material resulted in better growth of runner plantlets in propagation of 'Seolhyang' strawberry. The results of this research will be used for production of high quality runner plantlets in strawberry propagation.
Medium Depths and Fixation Dates of 'Seolhyang' Strawberry Runner Plantlets in Nursery Field Influence the Seedling Quality and Early Growth after Transplanting
Park, Gab Soon ; Choi, Jong Myung ;
Korean Journal of Horticultural Science and Technology, volume 33, issue 4, 2015, Pages 518~524
DOI : 10.7235/hort.2015.15004
The objective of this research was to investigate the influence of various depths of expanded rice hull (ERH) medium and fixation dates of runner plantlets of 'Seolhyang' strawberry on the growths in nursery field and in plastic house soil after transplanting. The five treatments in medium depths (30, 50, 70, 90, and 110 mm) and four treatments in fixation dates (1st and 15th July and 1st and 15th August) were tested. The growths of runner plantlets were investigated before transplanting to plastic house soil. The early growth and inflorescence rates of crops after transplant to plastic house soil were also investigated. The plant height and fresh weight of runner plantlets were the highest in the medium depths of 50, 70, and 90 mm. The medium depth of 30 mm had higher numbers of first roots, but lower root fresh weight compared to those of 70, 90, and 110 mm. The treatment of 30 mm in medium depth showed poorer growth in all indexes except root length and root weight compared to those of 70, 90, and 110 mm. The runner plantlets fixed on July 1 and July 15 showed good root growth and the weights of ERH adhered to form root balls were 18.3 g and 13.9 g, respectively. The detached amount of ERH was less than 40% in the two treatments when root balls were shaken by a vibratory sieve shaker. The plant growth at 45 days after transplanting to plastic house soil were not significantly different when the runner plantlets were fixed in the period from July 1 to Aug. 1. The inflorescence rates of the first cluster were 93 to 100% when runner plantlets were fixed in the period from July 1 to Aug. 1. By contrast the runner plantlets fixed on the Aug. 15 had a 67% in florescence rate for the first cluster. These results indicate that optimum depth of ERH medium was 7 cm and the ranges of optimum fixation dates are from July 20 to 25.
Effect of CaO Treatment on Quality Characteristics and Storage of Mulberry (Morus alba L.) Fruits in Yecheon
Yang, Ji-won ; Kim, Young Eon ;
Korean Journal of Horticultural Science and Technology, volume 33, issue 4, 2015, Pages 525~534
DOI : 10.7235/hort.2015.14198
The effects of aqueous calcium oxide (CaO) treatment on the quality characteristics and shelf life of mulberry (Morus alba L.) were investigated. Mulberry fruits were immersed in 0, 0.5, 1, and
CaO solutions for 0, 1, 3, 6, and 12 min. Mulberries were then rinsed with potable tap water for 1 min and stored at
for 14 days. CaO treatment was effective at promoting the retention of titratable acid, pH, and ascorbic acid as well as total flavonoid contents. CaO concentration and treatment time were significant factors affecting the sensory qualities of the fruits, including off-odor, flavor, and texture. For shelf life determinations, the total bacterial count was reduced by CaO treatment so that the samples treated with
CaO for 12 min had bacterial levels at 14 days comparable to those of the control at 4 day, and no coliform group was detected after CaO treatment. These results indicate that calcium oxide treatment is a promising approach for the preservation of mulberry fruit.
The Potassium to Magnesium Ratio Enables the Prediction of Internal Browning Disorder during Cold Storage of Asian Pears
Seo, Ho-Jin ; Chen, Po-An ; Lin, Shu-Yen ; Choi, Jin-Ho ; Kim, Wol-Soo ; Haung, Tzu-Bin ; Roan, Su-Feng ; Chen, Iou-Zen ;
Korean Journal of Horticultural Science and Technology, volume 33, issue 4, 2015, Pages 535~541
DOI : 10.7235/hort.2015.15050
'Taichung No. 2' is a new Asian pear cultivar developed in Taiwan with low chilling requirement; however, is likely to develop internal browning disorder under low temperature storage conditions. We investigated the impact of storage time on flesh browning disorder in pears harvested from 22 orchards in 2010 and 2011, and analyzed the levels of nutrients in different fruit parts such as the peel, flesh, and core. Calcium and potassium contents were higher in the flesh and peel, respectively, of more severely browned fruits, whereas a lower magnesium content was recorded in the peel and core of these fruits. Nitrogen and potassium contents in the peel, and calcium content in the flesh were positively correlated with browning disorder severity. By contrast, the magnesium content in the core was negatively correlated with browning disorder severity. However, the nutrient contents in fruits varied between the two sampling years considered. Only the K/Mg ratio was an effective predictor of the browning disorder severity and showed a positive linear correlation in the two years. We recommend that the K/Mg ratio should be lower than 10 to avoid severe browning disorder in pears.
Effect of Preharvest and Postharvest 1-Methylcyclopropene (1-MCP) Treatments on Fruit Quality Attributes in Cold-stored 'Fuji' Apples
Yoo, Jingi ; Kang, Bong Kook ; Lee, Jinwook ; Kim, Dae Hyun ; Lee, Dong Hoon ; Jung, Hee-Young ; Choi, DongGeun ; Choung, Myoung-Gun ; Choi, In Myung ; Kang, In-Kyu ;
Korean Journal of Horticultural Science and Technology, volume 33, issue 4, 2015, Pages 542~549
DOI : 10.7235/hort.2015.15057
This work was carried out to evaluate the effects of preharvest 1-methylcyclopropene (1-MCP,
) and postharvest 1-MCP (
) treatments on the fruit quality attributes of cold-stored 'Fuji' ( Malus domestica Borkh.) apples. Fruits were exposed to 0, 95, 125, or
at 3, 2, 1 weeks before harvest (WBH), and treated with 0 or
at harvest. Fruit was then stored for up to 180 days at
. Fruit fresh weight, Hunter's value a, internal ethylene concentration (IEC), flesh firmness, titratable acidity (TA), and soluble solids content (SSC) in fruit treated with
were not different from those of control fruit at harvest. During cold storage, flesh firmness and TA were higher in fruit treated with
at 2 and 3 WBH than in control fruit. IEC was 5.5-10.0% lower in fruit treated with
at 2 and 3 WBH compared with control fruit as storage duration progressed, while SSC was not affected. Furthermore, flesh firmness, TA, and IEC were affected neither by
treatments, compared with those fruit quality attributes at harvest. The correlation maps indicated that IEC was negatively correlated with firmness and TA, regardless of
application levels. In addition, positive correlations between fruit quality attributes were detected in treatments with
. Therefore, the results suggest that with a single application of
, a higher level of
application would help in retention of fruit quality attributes during cold storage.
Application of the Molecular Marker in Linkage Disequilibrium with Ms, a Restorer-of-fertility Locus, for Improvement of Onion Breeding Efficiency
Kim, Sujeong ; Kim, Sunggil ;
Korean Journal of Horticultural Science and Technology, volume 33, issue 4, 2015, Pages 550~558
DOI : 10.7235/hort.2015.15006
To analyze the linkage relationships among molecular markers recently reported to be linked to onion (Allium cepa L.) Ms, a restorer-of-fertility locus, in onion (Allium cepa L.), three single nucleotide polymorphism markers were converted into cleaved amplified polymorphic sequence (CAPS) markers based on onion transcriptome sequences and the rice genome database. Analysis of the recombinants selected from 4,273 segregating plants using CAPS and other linked markers demonstrated the jnurf13 and jnurf610 markers to perfectly co-segregate with the Ms locus. In contrast to jnurf13, the jnurf610 marker was not in perfect linkage disequilibrium with the Ms locus in diverse breeding lines. Thus, the jnurf13 marker and the marker for identification of cytoplasm types were utilized to enhance the efficiency of onion breeding through four applications. First, 89 maintainer lines containing the normal cytoplasm and homozygous recessive Ms genotypes were successfully identified from 100 breeding lines. Second, these two molecular markers were used to analyze the main sources of male-fertile contaminants frequently found in the male-sterile parental lines during F1 hybrid seed production. The majority of the contaminants contained heterozygous Ms genotypes, indicating that pollen grains harboring the dominant Ms genotype may have been introduced during propagation of the maintainer lines. Therefore, the genetic purity of the two maintainer lines was analyzed in the third application, and the results showed that both maintainer lines contained 13-21% off-types. Finally, the two markers were used to increase the seed yield potentials of two open-pollinated varieties containing sterile cytoplasms by removing the plants harboring homozygous recessive and heterozygous Ms genotypes.
Linkage Analysis of the Three Loci Determining Rind Color and Stripe Pattern in Watermelon
Yang, Hee-Bum ; Park, Sung-woo ; Park, Younghoon ; Lee, Gung Pyo ; Kang, Sun-Cheol ; Kim, Yong Kwon ;
Korean Journal of Horticultural Science and Technology, volume 33, issue 4, 2015, Pages 559~565
DOI : 10.7235/hort.2015.14070
The rind phenotype of watermelon fruits is an important agronomic characteristic in the watermelon market. Inheritance and linkage analyses were performed for three rind-related traits that together determine the rind phenotype: foreground stripe pattern, rind background color, and depth of rind color. The inheritance of the foreground stripe pattern was analyzed using three different
populations, showing that the striped pattern is dominant over the non-striped pattern. The inheritance analysis of the rind background color was performed using
populations of the '10909' and '109905', and the depth of rind color was analyzed using
populations of the '90509' and '109905'. Yellow color was found to be dominant over green color, and a deep color was dominant over the standard color. Linkage analysis of the three traits was conducted using three
populations in which two traits were segregating. Each pair of traits was inherited independently, which demonstrated that the three traits are not linked. Therefore, we propose a three-locus model for the determination of rind phenotype, providing novel insight that rind phenotype is determined by the combination of three genetically independent loci.
Single Nucleotide Polymorphisms linked to the SlMYB12 Gene that Controls Fruit Peel Color in Domesticated Tomatoes (Solanum lycopersicum L.)
Kim, Bichsaem ; Kim, Nahui ; Kang, Jumsoon ; Choi, Youngwhan ; Sim, Sung-Chur ; Min, Sung Ran ; Park, Younghoon ;
Korean Journal of Horticultural Science and Technology, volume 33, issue 4, 2015, Pages 566~574
DOI : 10.7235/hort.2015.15032
Yellow or transparent fruit peel color is caused by the accumulation or lack of naringenin chalcone (NG, C) in fruit peel and determines the red or pink appearance of tomato fruit, respectively. NGC biosynthesis is regulated by the SlMYB12 gene of the Y locus on chromosome 1, and DNA markers derived from SlMYB12 would be useful for marker-assisted selection (MAS) of tomato fruit color. To develop a gene-based marker, 4.9 kb of the SlMYB12 gene including a potential promoter region was sequenced from the red-fruited (YY) line 'FCR' and pink-fruited (yy) line 'FCP'. Sequence alignment of these SlMYB12 alleles revealed no sequence variations between 'FCR' and 'FCP'. To identify SlMYB12-linked single nucleotide polymorphisms (SNPs), 'FCR' and 'FCP' were genotyped using a SolCAP Tomato SNP array and CAPS markers (CAPS-456, 531, 13762, and 38123) were developed from the four SNPs (solcap_snp_sl_456, 531, 13762, and 38123) most closely flanking the SlMYB12. These CAPS markers were mapped using
plants derived from 'FCR'
'FCP'. The map positions of the fruit peel color locus (Y) were CAPS-13762 (0 cM) - 456 (11.09 cM) - Y (15.71 cM) - 38123 (17.82 cM) - 531 (30.86 cM), and the DNA sequence of SlMYB12 was physically anchored in the middle of CAPS-456 and CAPS-38123, indicating that fruit peel color in domesticated tomato is controlled by SlMYB12. A total of 64 SolCAP tomato germplasms were evaluated for their fruit peel color and SNPs located between solcap_snp_sl_456 and 38123. Seven SNPs that were detected in this interval were highly conserved for pink-fruited accessions and specific to transparent fruit peel traits, as depicted by a phenetic tree of 64 accessions based on the seven SNPs.
Isolation and Functional Identification of BrDSR, a New Gene Related to Drought Tolerance Derived from Brassica rapa
Yu, Jae-Gyeong ; Park, Young-Doo ;
Korean Journal of Horticultural Science and Technology, volume 33, issue 4, 2015, Pages 575~584
DOI : 10.7235/hort.2015.15056
Drought stress is a crucial environmental factor determining crop survival and productivity. The goal of this study was to clearly identify a new drought stress-tolerance gene in Brassica rapa. From KBGP-24K microarray data with the B. rapa ssp. pekinensis inbred line 'Chiifu' under drought stress treatment, a gene which was named BrDSR (B. rapa Drought Stress Resistance) was chosen among 738 drought-responsive unigenes. BrDSR function has yet to be determined, but its expression was induced over 6-fold by drought. To characterize BrDSR, the gene was isolated from B. rapa inbred line 'CT001' and found to contain a 438-bp open reading frame encoding a 145 amino acid protein. The full-length cDNA of BrDSR was used to construct an over-expression vector, 'pSL100'. Tobacco transformation was then conducted to analyze whether the BrDSR gene can increase drought tolerance in plants. The BrDSR expression level in T1 transgenic tobacco plants selected via PCR and DNA blot analyses was up to 2.6-fold higher than non-transgenic tobacco. Analysis of phenotype clearly showed that BrDSR-expressing tobacco plants exhibited more tolerance than wild type under 10 d drought stress. Taking all of these findings together, we expect that BrDSR functions effectively in plant growth and survival of drought stress conditions.
Optimalization of Flurprimidol Concentration for Improvement of Acclimatization Rate and In Vitro Growth Inhibition in Bioreactor Culture of Strawberry Plantlets via Bioreactor Culture
Kim, Hye Jin ; Lee, Jong Nam ; Kim, Ki Deog ; Kwon, Gi Bum ; Yoo, Dong Lim ; Lim, Hak Tae ; Yeoung, Young Rok ;
Korean Journal of Horticultural Science and Technology, volume 33, issue 4, 2015, Pages 585~590
DOI : 10.7235/hort.2015.14058
This study was conducted to improve the acclimatization rate of in vitro strawberry plantlets through bioreactor culture using the growth retardant flurprimidol. Different concentrations [0 (Control), 0.1, 0.5, 1.0, and
] of flurprimidol were added during bioreactor culture. After six weeks of treatments, various growth characteristics were investigated and in vitro plantlets were acclimated in the greenhouse. The growth rate of treated plantlets was much lower than that of control, and as the treatment concentration increased, the growth rate was much decreased. Shoots of plantlets treated with flurprimidol were shorter (2.2-3.7 cm) than those of control (7.9 cm). The number of roots per treated plant was around 11.6-34.2, compared with 51.8 in the control. Root length was also lower (0.88-3.08 cm) than control (4.36 cm). However, the number of new shoots and leaves increased in all treatments except for
concentration. The root was partially decayed in
concentration and was completely decayed in
. The survival rate in
concentrations was 100% and 23.3% respectively. After four weeks of acclimatization, the plantlets restarted growth, and growth characteristics of shoots and roots recovered to the levels of control, except for fresh weight. Based on our results, a concentration of
flurprimidol is appropriate for improvement of acclimatization rate of in vitro strawberry plantlets in bioreactor culture.
Annual Increase in Carbon and Nitrogen Stocks of Trees and Soils in a 'Niitaka' Pear Orchard Following Standard Fertilization Recommendations
Ro, Hee-Myong ; Choi, Jin-Ho ; Lee, Seo-Yeon ; Lee, Tae-Kyu ; Kim, Jong-Sung ; Park, Ji-Suk ; Choi, Jang-Jeon ; Lee, Min-Jin ;
Korean Journal of Horticultural Science and Technology, volume 33, issue 4, 2015, Pages 591~597
DOI : 10.7235/hort.2015.14191
We determined the total C and N stocks in trees and soils after 1 year of fertilization in an experimental orchard with 16-year-old 'Niitaka' pear (Pyrus pyrifolia Nakai cv. Niitaka) trees planted at
spacing on a Tatura trellis system. Pear trees were fertilized at the rate of 200 kg N, 130 kg P and
. At the sampling time (August 2013), trees were uprooted, separated into six fractions [trunk, main branches, lateral branches (including shoots), leaves, fruit, and roots] and analyzed for their total C and N concentrations and dry masses. Soil samples were collected from 0 to 0.6 m in 0.1 m intervals at 0.5 m from the trunk, air-dried, passed through a 2-mm sieve, and analyzed for total C and N concentrations. Undisturbed soil core samples were also taken to determine the bulk density. Dry mass per tree was 5.6 kg for trunk, 12.0 kg f or m ain branches, 15.7 kg for lateral branches, 5.7 kg for leaves, 9.8 kg for fruits, and 10.5 kg for roots. Total amounts of C and N per tree were respectively 2.6 and 0.02 kg for trunk, 5.5 and 0.04 kg for main branches, 7.2 and 0.07 kg for lateral branches, 2.6 and 0.11 kg for leaves, 4.0 and 0.03 kg for fruit, and 4.8 and 0.05 kg for roots. Carbon and N stocks stored in the soil per hectare were 155.7 and 14.0 Mg, respectively, while those contained in pear trees were 17.8 and
based on a tree density of 667 trees/ha. Overall, C and N stocks per hectare stored in the pear orchard were 173.6 and 14.2 Mg, respectively. Compared with results obtained in 2012, the amounts of C stocks have increased by
, while those of N stocks remained virtually unchanged (
Early Identification of Citrus Zygotic Seedlings Using Pollen-specific Molecular Markers
Jin, Seong Beom ; Yun, Su Hyun ; Park, Jae Ho ; Park, Suk Man ; Koh, Sang Wook ; Lee, Dong Hoon ;
Korean Journal of Horticultural Science and Technology, volume 33, issue 4, 2015, Pages 598~604
DOI : 10.7235/hort.2015.14200
This study was carried out to develop molecular techniques to allow the selection of zygotic seedlings in the early stage of the plant development. We identified 37 pollen-specific molecular markers from RAPD analysis and successfully used them for identification of the zygotic seedlings from various hybrid crosses. Three Satsuma mandarin cultivars ('Morita unshiu', 'Nangan 20' and 'Miyagawawase') were used as mother parents and seven cultivars ('Ponkan', 'Lee', 'Kinokuni', 'Shiranuhi', 'Tamnaneunbong', 'Shinyegam', and 'Sunburst' mandarins) served as pollen parents. PCR analysis showed that 2 primers could identify zygotic hybrid seedlings. Among them, an UBC-27 primer was used to identify the zygotic seedlings from hybrid crosses of "'Nangan 20'
'Kinokuni'" mandarin, "'Nangan 20
Ponkan'" mandarin and "'Miyagawawase
Sunburst'" tangerine. In total 29 out of 40 seedlings (73%), 9 out of 47 seedlings (19%), and 13 out of 45 (29%) were identified as zygotic seedlings, respectively. These results can show that the pollen-specific markers selected in this study can be used effectively for early identification of zygotic seedlings from Citrus hybrid crosses.
Stabilizing Soil Moisture and Indoor Air Quality Purification in a Wall-typed Botanical Biofiltration System Controlled by Humidifying Cycle
Lee, Chang Hee ; Choi, Bom ; Chun, Man Young ;
Korean Journal of Horticultural Science and Technology, volume 33, issue 4, 2015, Pages 605~617
DOI : 10.7235/hort.2015.15047
The ultimate goal of this research is to develop a botanical biofiltration system that combines a green interior, biofiltering, and automatic irrigation to purify indoor air pollutants according to indoor space and the size of biofilter. This study was performed to compare the stability of air flow characteristics and removal efficiency (RE) of fine dust within a wall-typed (vertical) botanical biofilter depending on humidifying cycle and to investigate RE of volatile organic compounds (VOCs) by the biofilter. The biofilter used in this experiment was designed as an integral form of water metering pump, water tank, blower, humidifier, and multi-level planting space in order to be suitable for indoor space utilization. As a result, relative humidity, air temperature, and soil moisture content (SMC) within the biofilter showed stable values regardless of three different humidifying cycles operated by the metering pump. In particular, SMCs were consistently maintained in the range of 27.1-29.7% during all humidifying cycles; moreover, a humidifying cycle of operating for 15 min and pausing for 45 min showed the best horizontal linear regression (y = 0.0008x + 29.09) on SMC (
) during 120 hour. REs for number of fine dust (PM10) and ultra-fine dust (PM2.5) particles passed through the biofilter were in the range of 82.7-89.7% and 65.4-73.0%, respectively. RE for weight of PM10 passed through the biofilter was in the range of 58.1-78.9%, depending on humidifying cycle. REs of xylene, ethyl benzene, total VOCs (TVOCs), and toluene passed through the biofilter were in the range of 71.3-75.5%, while REs of benzene and formaldehyde (HCHO) passed through the biofilter were 39.7% and 44.9%, respectively. Hence, it was confirmed that the wall-typed botanical biofilter suitable for indoor plants was very effective for indoor air purification.
Breeding of a Multi-flowering and Early-flowering White Calla Lily Cultivar 'White Cutie' Resistant to Soft Rot Disease
Joung, Hyang Young ; Cho, Hae Ryong ; Rhee, Ju Hee ; Shin, Hak Ki ; Park, Sang Kun ;
Korean Journal of Horticultural Science and Technology, volume 33, issue 4, 2015, Pages 618~623
DOI : 10.7235/hort.2015.14199
The new white calla lily (Zantedeschia aethiopica) cultivar 'White Cutie' was bred at the National Institute of Horticultural & Herbal Science (NIHHS) in 2011. 'Childsiana' showing the multi-flowering characteristic and 'Wedding March' resistant to soft rot disease were artificially crossed in 2004. Of the progeny, 'White Cutie' was selected specifically for use in cut flower production after investigation over seven years (2005 to 2011) of genetic and phenotypic characteristics, resistance against soft rot, and customer preference regarding the culture vigor and post-harvest quality. 'White Cutie' was early flowering (85.6 days to flowering) with white flowers (RHS W155C), although it had a mid-sized flower in which spathe height and width were 8.6 cm and 8.7 cm, respectively. It was multi-flowering (6.2 flowers per plant) and produced a very high number of cormels (13.4 per plant). Furthermore, it was resistant to soft rot disease.