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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Medicinal Crop Science
Journal Basic Information
Journal DOI :
The Korean Society of Medicinal Crop Science
Editor in Chief :
Volume & Issues
Volume 10, Issue 5 - Dec 2002
Volume 10, Issue 4 - Nov 2002
Volume 10, Issue 3 - Sep 2002
Volume 10, Issue 2 - Jun 2002
Volume 10, Issue 1 - Mar 2002
Selecting the target year
Isolation of the Phytophthora Root Rot Pathogen of Atractylodes macrocephala, Phytophthora drechsleri, and Bioassay of the isolates with Seedlings
Cho, Joon-Hyeong ; Kim, Yong-Wook ; Bang, Kyong-Hwan ; Park, Chun-Gun ; Seong, Nak-Sul ;
Korean Journal of Medicinal Crop Science, volume 10, issue 3, 2002, Pages 155~161
Using semi-selective (SS-) and selective (Jee-) medium, we identified the pathogens isolated from the symptomatic plants and soils collected from different locations, such as Suwon, Andong, and Youngju, as P. drechsleri, which is Phytophthora root rot causal agent of A. macrocephala. At
, these isolates were grown faster on 10% V8A (V8 juice agar) medium than on PDA (potato dextrose agar) with hyphal swelling, but no growing was observed at below
. In order to identify the pathogenicity of each isolate, seedlings of A. macrocephala were inoculated with mycelium -zoospore suspended inoculum, which was prepared by culturing on 10% V8A medium and homogenizing in distilled water. By this method, wide ranges of pathogenicity were observed as follows;
of disease severities concerning the lesion areas of the top plants and
of disease incidences. Therefore, this was considered as a efficient method to identify the pathogenicity of P. drechsleri in large scale screening. P-A200073, isolated from soils in Andong, and P-9755, from the root of symptomatic plant of A. macrocephala in Suwon, showed the highest degree of pathogenicity to the seedlings. By these isolates, lesion areas and disease incidences of the inoculated seedlings were occurred
, respectively. However, no symptoms were observed in uninoculated control. Same pathogens were reisolated from roots and lower stems of the inoculated plants, but not from leaves.
Variation of Volatile Composition in the Leaf of Zanthoxylum schinifolium Siebold et Zucc. & Zanthoxylum piperitum DC
Cho, Min-Gu ; Chang, Chin-Sung ; Chae, Young-Am ;
Korean Journal of Medicinal Crop Science, volume 10, issue 3, 2002, Pages 162~166
and citronellal showed seasonal variation in the leaves of Z. schinifolium. Especially estragole was detected at fruiting stage regardless of collection sites. Common variation components in the leaves of Z. piperitum at all collection sites were (Z)-3-hexenol,
, limonene and citronellal. The compositions with monthly variation in Z. schinifolium at arboretum were
, myrcene, (Z)-3-hexenyl acetate,
, linalool, geranyl acetate while in Z. piperitum were hexanal, (Z)-3-hexenol, (E)-2-hexenal, hexanol,
, (Z)-ocimene, limonene, citronellal, geranyl acetate,
. Estragole was not detected in Z. schinifolium leaves at arboretum due to too young tree to bearing fruit on it.
Effect of Extract Agrimonsa Pilosa L. on biological Activity in Rats
Lee, Yong-Ho ; Kim, Man-Bae ; Chung, Dae-Soo ;
Korean Journal of Medicinal Crop Science, volume 10, issue 3, 2002, Pages 167~170
Agromonia pilosa L. has been used as a medicinal plant in traditional folk remedy, and it shows increasing tendency at various sections such as medicine-making material, functional food, and agricultural chemicals using plant or extract. Dosage effect of extract from Agrimonia pilosa L. plant on rat performance experiments were summarized as follows : Body weight was increased with 0.02% dosage treated-rat by 5% as compared to non-treated one, however, decreasing tendency was observed with 0.04% extract of Agrimonia pilosa L. plant fed rat to control Considering feed efficiency was similar result between extract dosage with 0.02% and non-treated rat. The number of leucocyte was increased by dosage-treated level except 0.02% dosage-treated rat. Number of erythrocyte was increased with 0.06% extract dosage-treated rat by 20% as compared to non-treated one.
Screening for Antioxidative Activity of Oriental Medicinal Plant Materials
Lee, Seung-Eun ; Seong, Nak-Sul ; Park, Chun-Geun ; Seong, Jung-Suk ;
Korean Journal of Medicinal Crop Science, volume 10, issue 3, 2002, Pages 171~176
Antioxidative effect of 42 medicinal plant extracts was screened to search natural antioxidants. The extracts of Terminalia chebula, Caesalpinia sappan, Sanguisorba officinalis, Rubus coreanus, and Alpinia katsumadai showed strong inhibition effect on DPPH radical, and LDL oxidation. Those of Eugenia caryophyllata, Alpinia officinarum, Zingiber officinale, Xanthium strumarium, Sophora japonica, Aristolochia contorta, and Alpinia katsumadai exhibited potent antioxidative activity on linoleic acid peroxidation. Among 42 medicinal plant materials, the extract of Terminalia chebula showed the highest scavenging activity
on DPPH radical which was higher than that
. The extract of Terminalia chebula showed also stronger inhibition activity on LDL oxidation than that of
Effect of Water Extract of Ulmi pumilae Cortex on Gastric Ulcer in Rats
Lim, Jong-Pil ; Cui, Xun ;
Korean Journal of Medicinal Crop Science, volume 10, issue 3, 2002, Pages 177~180
Ulmi pumilae Cortex(bark of Ulmus pumila L.), oriental medicine, has been used for the folk remedy of the gastric diseases. In order to investigate antiulcer activities, some experiments for Shay, aspirin-induced and indomethacin-induced gastric ulcers were conducted. The water extract of Ulmi pumilae Cortex(UX) was given intraperitoneally, and the groups of UX 500 and 1,000mg/kg significantly inhibited Shay, aspirin and indomethacin-induced ulcers in rats.
Variation of Growth and Allylisothiocyanate contents of Wasabia japonica Matsum. Cultivar
Byeon, Hak-Soo ; Heo, Su-Jeong ; Lim, Soo-Jeong ; Seo, Jeong-Sik ;
Korean Journal of Medicinal Crop Science, volume 10, issue 3, 2002, Pages 181~184
This study was conducted to select the optimum cultivar of wasabi in water condition. In three cultivar, Daruma, Simanesarai and Daihoichigo, the Daruma was superior to the others. Though total rhizome weight was similar to Daruma and Daihoichigo, rhizome of main stem of Daruma was bigger than that of Daihoichigo, specially distribute in
size. The allylisothiocyanate content was higher in rhizome than that of leaf, lateral rhizome and petiole. In three cultivar, the allylisothiocyanate content of rhizome, Daihoichigo, Daruma and Simanesairai was 0.3389, 0.6332, 0.3956(mg/g), respectively.
Major Chemical Components in Rhizome of Native Polygonatum Species in Korea
Jang, Kye-Hyun ; Kang, Jin-Ho ; Jeon, Byeong-Sam ; Song, Geun-Woo ; Lee, Sang-Dae ;
Korean Journal of Medicinal Crop Science, volume 10, issue 3, 2002, Pages 185~193
This study was done to accept basic data on chemical components in rhizome of native Polygonatum species in Korea. In mineral contents of their rhizome, total nitrogen, potassium, calcium, magnesium and sodium concentration were higher in Polygonatum inflatum, P. humile and P. odoratum var. pluriflorum 1 than the other species in all the sampling dates. P. odoratum var. pluriflorum 1, P. odoratum var. pluriflorum 2 and P. inflatum showed relatively higher iron and mananese contents but P. odoratum var. pluriflorum 1 and P. stenophyllum did in copper content on November 20. Total sugar was acculated in later growth stages, although P. stenophyllum had the highest content but P. lasianthum var. coreanum had the lowest in all the sampling dates. Protein content was the highest in P. lasianthum var. coreanum but the lowest in P. stenophyllum in all the sampling dates. The major amino acids in their rhizomes were arginine followed by glutamic acid, and total and essential amino acid content were abundant in P. lasianthum var. coreanum and P. humile.
Analysis of Genetic Relationship by RAPD Technique for Codonopsis lanceolata Trauty Collected from the Baekdoo Mountain and Korea
Doo, Hong-Soo ; Ryu, Jeom-Ho ; Lee, Kang-Soo ; Li, Hu Lin ; Liu, Xian Hu ;
Korean Journal of Medicinal Crop Science, volume 10, issue 3, 2002, Pages 194~199
Extracted genomic DNA from 16 accessions of Codonopsis lanceolata collected from South Korea and the Baekdoo Mt. areas of China were analyzed for their genetic relationships by RAPD. Twenty 10-mer-oligonucleotide primers having reproductive polymorphism were selected for the RAPD analysis. The size of amplified DNA was almost between 125 bp and 2.0 kbp. Sixteen collected Codonopsis lanceolata were analyzed with 20 primers which generated 73(49.3%) polymorphic bands among 148 PCR products. The mean number of polymorphic bands were 7.4 and varied
per primer. It was, thus, demonstrated that RAPD was useful for detecting polymorphism in Codonopsis lanceolata. The range of 1-F value(genetic similarity) was from 0.682 to 0.959. These results indicate variable genetic similarities. By UPGMA (Unweighted Pair Group Method using an Arithmetic average) cluster analysis based on 1-F value, genetic distance among the 16 collected Codonopsis lanceolata was
. It was certainly classified into two groups between collected accessions from Korea and China, and the genetic distance was about 0.281. Both accessions collected from Korea and China showed miner differences, while the genetic relationships of Tonghua Xian and Liuhe Xian from China was farthest with other accessions collected.
Quantitative Analysis of Platycodin D from Platycodon grandiflorum by HPLC-ELSD
Kim, Geum-Soog ; Kim, Hyun-Tae ; Seong, Jae-Duck ; Park, Hee-Saeng ; Kim, Soo-Dong ;
Korean Journal of Medicinal Crop Science, volume 10, issue 3, 2002, Pages 200~205
Platycodin D was isolated from n-butanol extract of Platycodi radix(Platycodon grandiflorum and identified by the spectroscopic analysis using
NMR techniques. A new method of analysis of platycodin D by high performance liquid chromatography(HPLC) was established using a reversed phase system with YMC-Pack ODS-AM( 250 X 4.6 mm) column and 30% acetonitrile as a mobile phase. Evaporative light scattering detector was used as detector. The kinds of extraction solvents and methods were examined to determine the efficient extraction condition and HPLC analysis was carried out to establish the optimum drying condition for the root of Platycodon grandiflorum. The contents of Platycodin D was highest as 0.083% when platycodon roots were dried at
using dry oven.
Antimicrobial Activity of Essential Oils from Mentha arvensis L. var. piperascens Malivaud and Agastache rugosa O. Kuntze on Escherichia coli and Salmonella typhimurium
Lee, Seung-Eun ; Park, Chun-Geon ; Cha, Moon-Seok ; Kim, Jin-Kyong ; Seong, Nak-Sul ; Bang, Kyong-Hwan ; Bang, Jin-Ki ;
Korean Journal of Medicinal Crop Science, volume 10, issue 3, 2002, Pages 206~211
For developing natural presevatives, essential oils of Mentha arvensis L. var. piperascens Malivaud and Agastache rugosa O. Kuntze were analyzed the composition of two oils and experimented on microorganism survival. Main components of Mentha arvensis oil were isomenthol (26.84%) and menthol (25.48%), and those of Agastache rugosa oil were estragole (79.83%) and limonene (4.13%) from GC-MSD analysis. Inhibition activities of Mentha arvensis oil against growth of Escherichia coli O157 : H7 ATCC 43895 and Salmonella typhimurium ATCC 7988 were observed from their clear zone
, and that of Agastache rugosa oil were done from the clear zone
by concentration-dependent manner, respectively. In the inhibition test on CFU/ml of the microorganisms, both of the plant essential oils at concentration of 5 and 10 mg showed potent growth inhibition activities from 9 hour of incubation. Analysis using transmission electron microscope on E. coli also showed antimicrobial activities of the oils as deformation of the cell and loss of the intracellular materials.
Comparison of biological activities of MeOH extracts in different cultivars and organs of Catharanthus roseus (L.) G.Don
Lee, Hui-Kyoung ; Kim, Ju-Sung ; Kim, Myong-Jo ; Heo, Kwon ; Lee, Hyeon-Yong ; Yu, Chang-Yeon ;
Korean Journal of Medicinal Crop Science, volume 10, issue 3, 2002, Pages 212~216
Catharanthus roseus was extracted with methanol and its Vinblastine contents and antioxidant and antimicrobial activities in each methanol extracts were determined. Vinblastine contents of leaves were much higher than those in other plant parts. The highest DPPH(1,1-diphenyl-2 -picrylhydrazy) radical scavenging activities in plnat parts showed in leaves of Stardust Orchid and Cooler Rose and the value was
respectively. Leaves of Cooler Rose and Coller Strawberry showed strong antimicrobial activities. Particularly, Cooler Rose showed strong antimicrobial activites except to Penicillium oxalicum.
Effect of Genotype and Explant on Somatic Embryogenesis and Acclimatization of Acanthopanax senticosus
Lee, Cheng-Hao ; Yu, Chang-Yeon ;
Korean Journal of Medicinal Crop Science, volume 10, issue 3, 2002, Pages 217~221
Callus induction and embryogenesis were studied in three different genotypes of Acanthopanax senticosus, to develop a protocol for somatic embryogenesis and acclimatization. Young leaf, stem, node, petiole, peduncle, flower and root explants were collected from 3-year old trees of A. senticosus accessions (Korea, Russia and Japan). Callus was obtained from all cultured explants but showed the higher rate of callus formation in flower cultured. For the three A. senticosus accessions, callus was well formd on MS media containing 2mg/ l of 2,4-D and 2mg/ l of TDZ, 4mg/ l of 2,4-D and 1mg/ l of TDZ than other treatments. For three A. senticosus accessions, when callus transferred to MS medium with 2,4-D, embryogenic cell formed. For A. senticosus accessions Korea, embryogenic cells were obtained on callus induced from petiole, stem, node and root explants, and induction rate was lower than 3%. 200mg of embryogenic callus was transferred to MS free liquid medium and somatic embryos of heart stage were obtained after 45days of culture. When somatic embryo of germination stage were transferred to solid medium, most of the embryos were regenerated into plantlets on 1/4 MS medium. Normal plants with both shoots and roots were transferred to greenhouse soil and were successfully acclimatized.
Effect of Antioxidant, Antimutagenicity and Anticancer of Root Extract from Ixeris dentata Nakai
Kim, Myong-Jo ; Kim, Ju-Sung ; Jeong, Dong-Myong ; Ham, Seung-Shi ; Yu, Chang-Yeon ;
Korean Journal of Medicinal Crop Science, volume 10, issue 3, 2002, Pages 222~229
Ixeris dentata root were extracted with methanol and then fractionated with n-hexane, EtOAc and BuOH to get active fractions. and their antioxidant and antimicrobial activities in each fraction were determined. Ethyl acetate fraction of Ixeris dentata root showed strong antioxidant activities, but aqueous fraction did not show any activities. But in the antimicrobial test, aqueous fraction showed strong antimicrobial activities except to Escherichia coli. especially, aqueous fraction showed the strongest activities against Hypocrea nigricans. and butanol fraction showed the strongest activities against Cladosporium herbarum. This study was performed to determine the antimutagenic and cytotoxic effect of Ixeris dentata root methanol extract on Salmonella typhimurium TA98, TA100 and cancer cell lines using ames test and cytotoxicity assay, respectively. Cancer cell lines include human lung carcinoma(A549), human breast adenocarcinoma(MCF-7) and human hepatocellular carcinoma (Hep 3B). Futher fractionations with hexane, ethyl acetate, butanol and water from methanol extract of Ixeris dentata root were performed to obtain effective fraction, methanol extracts showed 79.94% inhibitory effect on the mutagenesis induced by N' -methyl- N' -nitro-N-nitrosoguanidine(MNNG) against TA100, while 89.99% inhibition was observed on the mutagenesis induced by 4-nitroquinoline-1-oxide(4NQO) against TA98. In the meanwhile, butanol fraction showed 89.92% and 71.01% inhibitory effect on the mutagenesis induced by benzo(a)pyrene(B(a)P) against TA98 and TA100, respectively. Ethyl acetate fraction showed the strongest effect against A549, MCF-7 and Hep3B at the same concentration compared to those of other fration.