Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Medicinal Crop Science
Journal Basic Information
Journal DOI :
The Korean Society of Medicinal Crop Science
Editor in Chief :
Volume & Issues
Volume 10, Issue 5 - Dec 2002
Volume 10, Issue 4 - Nov 2002
Volume 10, Issue 3 - Sep 2002
Volume 10, Issue 2 - Jun 2002
Volume 10, Issue 1 - Mar 2002
Selecting the target year
The Effect of Polysaccharide from Angelica Gigas Nakai on Controlling the Differentiation of Human Embryonic Stem Cells
Park, Young-S. ; Lee, Jae-E. ; Lee, Seo-H. ; Lee, Hyeon-Y. ;
Korean Journal of Medicinal Crop Science, volume 10, issue 4, 2002, Pages 237~242
It was found that the purified extract from A. gigas Nakai (polysaccharide, M.W., 25 kD) controled differentiating human ES cells. Its optimal supplementation concentration was decided as 0.8
to efficiently control the differentiation. It also enhanced the cell growth, compared to the control. However, most widely used and commercially available differentiating agent, Leukemia Inhibitory Factor (LIF) negatively affected on the cell growth even though it controls the differentiation of ES cells, down to 40-50 % based on morphological observation and telomerase activity. It was presumed that the extract first affected on cell membrane and resulted in controlling signal system, then amplify gene expression of telomere, which enhanced the telomerase activity up to three times compared to the control. LIF only increased the enzyme activity up to two times. It was confirmed that the extract from A. gigas Nakai could be used for substituting currently used differentiation controlling agent, LIF from animal resources as a cheap plant resource and not affecting the cell growth. It can broaden the application of the plants not only to functional foods and their substitutes but also to fine chemicals and most cutting-edge biopharmaceutical medicine.
Optimization of Major Culture Elements on Growth and Shikonin Production in the Lithospermum erythrorhizon Hairy Root Culture
Hwang, Ok-Jin ; Kim, Yu-Jeong ; Sung, Nak-Sul ; Ahn, Jun-Cheul ; Kim, Sik-Eung ; Hwang, Baik ;
Korean Journal of Medicinal Crop Science, volume 10, issue 4, 2002, Pages 243~248
The effects of basal media, carbon, nitrogen, phosphate and some major macro elements on growth and shikonin production in Lithospermum erythrorhizon hairy root culture were studied. Among examined media, growth of hairy root cultured in B5 liquid medium was rapid, whereas shikonin production was high in MS liquid medium. Under B5 basal medium, sucrose concentration for optimal growth and shikonin production was 9% and 4% respectively. The growth and shikonin production on pH changes in B5 medium resulted little effect in pH 5.8 to pH 8.8 ranges, whereas growth was decreased dramatically in both above 8.8 and under 5.8. Nitrogen source and concentration effected on the growth and shikonin production. The highest growth rate was in B5 medium (50 mM
and 1 mM
, whereas the highest shikonin production was in the condition supplemented with 5 mM
and 10 mM
Micropropagation of Kalopanax
by bud cultrue
Kim, Bong-Kyu ; Yi, Yong-Sub ; Ahn, Joong-Hoon ;
Korean Journal of Medicinal Crop Science, volume 10, issue 4, 2002, Pages 249~252
Several plant growth regulators were examined for on their effect on the in vitro propagation of Kalopanax pictus
on WPM medium. Among the cytokinins tested, BA at
appeared to be the most effective for multiple axillary shoot formation. Although the addition of
promoted stem elongation, it produced morphologically abnormal leaves and stems. For rooting of the shoots,
IBA seemed to be more effective than
NAA. When the regenerated plants were transferred on artificial mixture containing vermiculite and peat-moss (1 : 1, v/v), 81% of them survived and grew normally.
Effects of Buckwheat, Potato and Rice on Glycemic Indices in Healthy Subjects
Koh, Eun-Sook T. ; Ju, Jin-Soon ; Choi, Moon-Gi ; Yoon, Tae-Heon ; Ahn, Young-Sook ; Lim, Kyung-Ja ; Kim, Soon-Ok ; Kim, Jong-Dai ;
Korean Journal of Medicinal Crop Science, volume 10, issue 4, 2002, Pages 253~258
We compared the long-term metabolic effects of equal amounts of carbohydrate from potato, rice and buckwheat on glycemic indices and blood lipids in healthy subjects. Nine healthy volunteers-2 men and 7 women were studied. All subjects ate diets based on the same-7-day rotating menu differing only in that the major source of carbohydrate (about 50% of daily total calories) came either from buckwheat, rice or potato. The study was conducted with a triple crossover design over three 7 day periods. On the morning of the 8th day, fasting blood was drawn from each subject to determine serum glucose, insulin, triglycerides, total and HDL-cholesterol. Subjects were then asked to eat breakfast with their respective carbohydrate within a 20 min period. Blood samples were drawn at 30, 60, 120 and 180 min after the start of breakfast to determine glucose and insulin levels. At 30 min the glucose response to the rice meal(7.15mmol/L) and potato meal(6.71mmol/L) were greater than the response to the buckwheat meal(5.855mmol/L) (P < 0.05). The mean area under the glucose response to the curve following the rice meal was greater than that following the buckwheat meal(P < 0.05). The insulin responses to the potato and rice meals at 30 and 60 min were greater than those to the buckwheat meal (P < 0.05). The mean area under the serum insulin response curve after the rice meal was greater than of buckwheat. Blood lipids, uric acid and glycosylated hemoglobin were not affected by the three meals. The study shows that the buckwheat meal has more beneficial effects on glycemic indices than either the rice meal or potato meal in healthy subjects.
In vitro Callus formation and Plant Regeneration of Epimedium koreanum Nakai
Yu, Chang-Yeon ; Kim, Na-Young ; Kang, Won-Hee ; Kim, Myong-Jo ; Heo, Kwon ; Choi, Won-Cheol ; Lee, Hyeon-Yong ;
Korean Journal of Medicinal Crop Science, volume 10, issue 4, 2002, Pages 259~262
The efficiency of regeneration of callus and explants from leaf and stem disks of Epimedium koreanum was examined on the MS media containing 2,4-D, NAA, Kinetin, BA and TDZ. Calli were formed on the 2mg/l 2,4-D media at the rate of 32% from leaf discs and 52% from stems. No callus was produced on the media which are containing BA or TDZ alone. The combination of 2,4-D and BA showed the effect on the formation of callus. The combination of 2mg/l 2,4-D and 0.lmg/l BA in the MS media had produced the highest percentage of callus formation, 50% from leaf discs and 40% from stems, respectively. The combination of 2mg/l 2,4-D and 1mg/1 BA in the MS media had affected the formation of callus in the rate of 40% from leaf discs and 25% from stems. The combined plant growth regulators of 2,4-D and BA increased the formation of calli from leaf discs, but single treatment of 2,4-D showed the highest callus formation from stems. Multiple shoots from leaf discs were formed on the media containing NAA, BA, kinetin, and TDZ. The highest number of multiple shoots were obtained 0.1mg/l NAA combined with 1mg/l kinetin. As a result, leaf discs or stems can be used for the mass propagation of Epimedium koreanum, but stem elongation of shoots from calli was not easy.
In vitro Selection of Acifluorfen-tolerant Solanum ptycanthum and Phenotypic Variation in Regenerated Plants
Yu, Chang-Yeon ; Lim, Jeong-Dae ; Kim, Myong-Jo ; Kang, Won-Hee ; Hyun, Tae-Kyoung ;
Korean Journal of Medicinal Crop Science, volume 10, issue 4, 2002, Pages 263~268
Acifluorfen-tolerant callus lines of Solanum ptycanthum were isolated by stepwise selection. Growth of the unselected line was completely inhibited at 0.5 uM. while some selected lines grew at 8 uM acifluorfen. Twenty-two of twenty-five acifluorfen-tolerant callus lines regenerated shoots. Many of the regenerated somaclones were variants, differing in leaf shape, leaf color, number of flower parts, flower color, and fertility. The acifluorfen tolerant S. ptycanthum callus lines differed.
In vitro Antioxidant Activity and Anticancer Effects of The Extracts from Eleutherococcus senticosus Max
Kim, Myong-Jo ; Kim, Na-Young ; Kang, Won-Hee ; Choi, Won-Cheol ; Yu, Chang-Yeon ;
Korean Journal of Medicinal Crop Science, volume 10, issue 4, 2002, Pages 269~272
Ethanol and butanol fractionation of E. senticosus showed strong anti-oxidant activity, and methanol and water extracts also had high anti-oxidant activity. The anti-oxidant activities in ethanol and butanol fractionation were higher than or similar to those of
. The cytotoxic effect of root extract of E. senticosus was evaluated on seven different human cancer cell lines, The extracts of leaf and stem of E. senticosus also had strong antioxidant activity, but the antioxidant activity in root extract was higher than those in leaf and stem extracts. Methanol, hexane, and aqueous fraction layer had much higher inhibitory activities on lipid peroxidation in rat liver microsomes compared with
. The effect of root extract of E. senticosus was evaluated on six human cancer cell lines. The values of 50% growth inhibition
for the extracts were mostly below
, and the extracts are considered as active inhibitory compounds on cancer cells.
Selection of disease resistant lines of Rehmannia glutinosa with mutant treatment
Yu, Chang-Yeon ; Lee, Youn-Su ;
Korean Journal of Medicinal Crop Science, volume 10, issue 4, 2002, Pages 273~276
Rehmannia glutinosa is one of the most important medicinal crops in Korea. However, various plant pathogens including Fusarium sp. cause great damages and cause enormous economic losses. Therefore, this study was conducted to select Fusarium resistant plants by using mutagen-treated Rehmannia glutinosa. The plant material used was a native accession of Rehmannia glutinosa. As a result, among the Rehmannia glutinosa treated with various concentrations of EMS, R. glutinosa treated with 0. 03M EMS showed higher resistance against infection of F. oxysporum in pot tests, and Rehmannia glutinosa plants treated with 0.12M EMS showed higher resistance against infection of F. oxysporum in field tests.
Medicinal Plants from Central and Western Asia
Zaurov, David E. ; Simon, James E. ; Park, Chung-Heon ; Letchamo, Wudeneh ; Wu, Qing-Li ; Wang, Mingfu ; Belolipov, Igor V. ; Sodombekov, Ishenbay ;
Korean Journal of Medicinal Crop Science, volume 10, issue 4, 2002, Pages 277~283
Inhibitory Effects of the Extract of Rhus verniciflua Stokes on the Reverse Transcriptase of AIDS
Kim, Myong-Jo ; Choi, Won-Cheol ; Barshinikov, A. M. ; Kobayashi, A. ;
Korean Journal of Medicinal Crop Science, volume 10, issue 4, 2002, Pages 284~287
Four olefinic catechols, commonly referred to as urushiol were isolated from the sap of Korean Rhus verniciflua Stokes and had the stronger inhibitory effects on the reverse transcriptase of AIDS. The hexane extract with a inhibitory effects on reverse transcriptase was purified by silica and ODS gel column chromatography. The active compounds were identified by MS and
as 3-[8' (Z), 11' (Z), 14' -pentadecatrienyl]catechol, 3-[8' (Z), 11' (Z)-pentadecadienyl]catechol, 3-[8' (Z)-penta- decenyl]catechol, and 3-pentadecylcatechol. All of these compounds showed strong inhibitory effects on reverse transcriptase of AIDS, in which 3-pentadecylcatechol exhibited the highest activity
Anticancer and Antioxidant Activity of Allergen-Removed Extract in Rhus verniciflua Stokes
Kim, Myong-Jo ; Choi, Won-Cheol ; Barshinikov, A. M. ; Kobayashi, A. ;
Korean Journal of Medicinal Crop Science, volume 10, issue 4, 2002, Pages 288~293
Allergen-removed-extract was produced from Rush verniciflua by two phase methods. Phase one was high temperature treatment of Rush verniciflua tree to get allergen-removed-extract. Phase two was extraction of solution from phase one product using water or organic solvents. The solutions from above method show high antioxidant activity, anticancer activity, and improvement in lung function, but did not contain urushiol family compounds.
Cloning and expression of glutathione S-transferase (GST) cDNA from Gossypium hirsutum L.
Kang, Won-Hee ; Kim, Myong-Jo ; Lim, Jung-Dae ; Yun, Song-Joong ; Chung, Ill-Min ; Yu, Chang-Yeon ;
Korean Journal of Medicinal Crop Science, volume 10, issue 4, 2002, Pages 294~297
A gene coding for the GST of cotton (Gh-5) was cloned into Escherichia coli and experssed. The enzyme remained within the cytoplasm of E. coli. An 696 bp open reading frame was in the 988 base pair fragment of the recombinant plasmid pET-30b(+). The deduced protein sequence consists of 232 amino acids and has a molecular mass of 30235.58 Da. The cloned enzyme conjugated reduced glutathione and 1-chloro-2,4-dinitrobenzene (CDNB). Plant GST cDNA was expressed in microbe and produced polypeptide had function as an enzyme.
Variation of the Essential oil and Main Component from Different Origin of Levisticulum officinale Koch
Chung, Hae-Gon ; Kim, Seong-Min ; Nemeth, E. ;
Korean Journal of Medicinal Crop Science, volume 10, issue 4, 2002, Pages 298~302
The essential oils were isolated by hydrodistillation and their composition determined capillary GC method with standards. The essential oil content showed significant differences between the two populations on the vegetative organs. The essential oil level of the leaves and roots was considerably higher in the Korean population at full flowering and waxy ripening stage but essential oil content of the roots was significantly higher in the Hungarian taxon at leaf rosette stage. We observed the essential oil accumulation tendency was mianly dependent on plant organs and intra-specific taxon during the vegeation period. Butylidene-phthalide was proved to be the main component of the oil in both population roots (50.9-73.3%), while dimethyl-acetate was showed as a major compound on the over-ground parts (56.7-62.0%). The qualitative composition of the essential oil in the reproductive organs concerning the identified compounds was the same as the vegetative parts with the main component
(4.8-28.1%) and butylidene-phtalide (9.7-16.1%), The quantitative composition showed some changes during the ontogenesis phases. Most characteristic ones are the decreasing proportion of dimethyl-acetate (from 7.3% to 1.1%) and the appearance of
(from 0.5% to 1.5%) only after fruit setting in both population.
Challenges and Tribulations in the Commercialization of New Crops: Aromatic and Medicinal Plants
Simon, James E. ; Jefthas, Elton ; Longenhaven, Petrus ; Smith, Marianna ; Renaud, Erica ; Juliani, Rodolfo ; Wang, Mingfu ; Zimba, Noah ; Acquaye, Dan ; Park, Chung-Heon ; Raskin, Ilya ; Tannous, Pierre ;
Korean Journal of Medicinal Crop Science, volume 10, issue 4, 2002, Pages 303~313