Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Medicinal Crop Science
Journal Basic Information
Journal DOI :
The Korean Society of Medicinal Crop Science
Editor in Chief :
Volume & Issues
Volume 11, Issue 5 - Dec 2003
Volume 11, Issue 4 - Nov 2003
Volume 11, Issue 3 - Sep 2003
Volume 11, Issue 2 - Jun 2003
Volume 11, Issue 1 - Mar 2003
Selecting the target year
Isolation and Structure Elucidation of Radical Scavengers from Chrysanthemum boreale Makino
Han, Wan-Soo ;
Korean Journal of Medicinal Crop Science, volume 11, issue 1, 2003, Pages 1~4
Two antioxidative compounds in Chrysanthemum boreale were isolated by a bioassay using a 2, 2-diphenyl-1-picrydrazyl(DPPH) free radical. They were identified as apigenin, linarin(acacetin-7-O-rutinoside) on the basis of
and MS data. The DPPH radical scavenging activity
of apigenin was similar to that of
and L-ascorbic acid
Screening of biological activities of the extracts from Rubus coreanus Miq
Lee, Mi-Kyoung ; Lee, Hyun-Soo ; Choi, Geun-Pyo ; Oh, Deog-Hwan ; Kim, Jong-Dai ; Yu, Chang-Yeon ; Lee, Hyeon-Yong ;
Korean Journal of Medicinal Crop Science, volume 11, issue 1, 2003, Pages 5~12
The biological activities of extracts from Rubus coreanus Miq. were compared. About 70% of the growth of human hepatocarcinoma and 79% of human gastric cancer cell was inhibited in adding 1.0 mg/ml of the extracts of Rubus coreanus Miq. respectively. The growth of human breast cancer cells was also inhibited in adding 1.0 mg/ml of the extracts as well as 78% of the human cancer cells. It was proved that the growth of human normal lung cell, scored as 15% for the extracts. Overall selectivity of the extracts on several human cancer cell line was over 5, which is higher than those from the Rubus coreanus Miq. The growth of both human immune B and T cells was enhanced up to 1.4 to 1.8 times by adding the extracts, compared to the controls. The secretion of tumor necrosis
from T cell was also increased up to 78.8 pg/ml in adding the ethanol extract (0.5 mg/ml). Ethanol extract also increased up to about 70 pg/ml of interleukin-6(IL-6) from B cell. For screening regulate function of blood pressure, angiotensin converting enzyme(ACE) activity was inhibited up to 25% by adding the ethanol extract (1.0 mg/ml). In testing the hypoglycemic activity, 20% of
activity was inhibited for the extracts (0.5 mg/ml). GST activity was increased in the range of 1.2 to 1.6 times by adding extracts.
Screening of immune enhancement activities of the extracts from Rosa rugosae Radix
Lee, Mi-Kyoung ; Lee, Seo-Ho ; Choi, Geun-Pyo ; Yu, Chang-Yeon ; Lee, Sin-Yeong ; Lee, Jin-Ha ; Lee, Hyeon-Yong ;
Korean Journal of Medicinal Crop Science, volume 11, issue 1, 2003, Pages 13~18
The biological activities of extracts from Rosa rugosae Radix were compared. About 78% of the growth of human hepato- carcinoma and 68% of human gastric cancer cell was inhibited in adding 0.5 mg/ml of the extracts of Rosa rugosae Radix respectively. The growth of human breast cancer cells was also inhibited in adding 0.5 mg/ml of the extracts as well as 66% of the human cancer cells. It was proved that the growth of human normal lung cell, scored as 20% for the extracts. Overall selectivity of the extracts on several human cancer cell line was over 4, which is higher than those from the Rosa rugosae Radix. The growth of both human immune B and T cells was enhanced up to 1.2 to 1.5 times by adding the extracts, compared to the controls. The secretion of tumor necrosis factor-alpha
from T cell was also increased up to 61.9 pg/ml in adding the ethanol extract (0.5 mg/ml). Ethanol extract also increased up to about 61.3 pg/ml of interleukin-6(IL-6) from B cell.
The extraction condition of pungent compounds from Zanthoxylum piperitum D.C pericarps by using supercritical fluid extraction
Lee, Chang-Joo ; Kim, Myung-Seok ; Shen, Jing-Yu ; Kim, Yong-Doo ; Shin, Jae-Han ;
Korean Journal of Medicinal Crop Science, volume 11, issue 1, 2003, Pages 19~23
The optimum extraction condition of pungent component from Zanthoxylum piperitum D.C pericarps by using supercritical fluid extraction(SFE) was investigated. The optimum condition of SFE was
of extraction temperature, 80% of
fluid, 20% of modifier(methanol) volume and 20 min of extraction time. The extraction efficiency between the classical solvent extraction method and SFE was studied. About 40% of extraction efficiency was improved when SFE was applied.
Screening of Plant Resources of anti-viral activity
Kwon, Dur-Han ; Kim, Man-Bae ; Yoon, Do-Young ; Lee, Young-Hee ; Kim, Jae-Wha ; Lee, Hee-Gu ; Choi, In-Seong ; Lim, Jong-Seok ; Choe, Yong-Kyeung ;
Korean Journal of Medicinal Crop Science, volume 11, issue 1, 2003, Pages 24~30
Aqueous extracts from eleven species of Korean medicinal plants were screened for antiviral activity against transmissble gastroenteritis virus (TGEV) and porcine epidemic diarrhae virus (PEDV). Seven of eleven plant extracts were found to have cytotoxicity for ST cells at the concentration of 1mg/ml, and ten extracts except Ephedra sinica were found to have non-cytotoxicity for Vero cells at the same concentration, Extracts of Zanthoxylum piperium, Cudrina tricuspidata, Clerodendron trichomum, Sophora flavescens, effectively inhibited TGEV, respectively. There were the antiviral activity against TGEV in the extracts of Glesdistsia sinensis, Euonymus alatus, Ficus carica, Solanum nigrum, Ephedra sinica, Xanthium strumarium, Acanthopanax seeliflorus in spite of their cytotoxicities. Nine extracts except Xanthium strumarium and Acanthopanax seeliflorus were found to have the antiviral activitity against PEDV. Extracts of Zanthoxylum piperium, Sophora flavescens was shown to have antiviral activities against both TGEV and PEDV.
The activity of antioxidants and suppression of cancer cell proliferation in extracts of Orostachys japonicus A. Berger
Kim, Chung-Hyun ; Park, Jae-Ho ; Lim, Jong-Kuk ; Lee, Kon-Joo ; Chung, Gyu-Young ; Jeong, Hyung-Jin ;
Korean Journal of Medicinal Crop Science, volume 11, issue 1, 2003, Pages 31~39
The antioxidative activities and biological properties in the EtOAc extracts and purified extracts of Orostachys japonicus were measured by assay of DPPH, xanthine/xanthine oxidase and mammalian cells(2-12). Scavenging of DPPH radical and inhibition of xanthine/xanthine oxidase of EtOAc extracts were showed the highest activity in the arable land and in September. The S-4 fraction showed the highest activity among the silica-gel column chromatography fractions. LH-4 fraction showed higher activity than the other fractionsins in assay of DPPH and xanthine/xanthine oxidase. Fatty acids and phenolic compounds were identified by GC/MS and main compounds were 1,2,3-benzenetriol, alpha-androsta-7,14-diene in LH-4 fraction. The activities of POD and SOD in samples havested on different habitats were high such as arable land> intermountain> seashore. That of POD and SOD in crude extracts of late stage were higher than early stage. Isozyme bands of crude extracts samplinged in all habitats and all growing stages showed two bands and the signal intensity showed strongly according to passage of growing stage. The purified extracts of LH-4 fraction showed excellent inhibition effect in proliferation of HL-60 cells and markedly suppressed colony formation in mouse fibroblast cells. Dose response between partially purified extracts(400ppm) and negative control did not produced statistically significant reduction in colony formation.
Analysis of Volatile Compounds in Leaves and Fruits of Zanthoxylum schinifolium Siebold et Zucc. & Zanthoxylum piperitum DC. by Headspace SPME
Cho, Min-Gu ; Kim, Hui ; Chae, Young-Am ;
Korean Journal of Medicinal Crop Science, volume 11, issue 1, 2003, Pages 40~45
Volatile components in the leaves and fruits of Z. schinjfolium and Z. piperitum were analyzed by Headspace SPME(Solid phase Microextraction). Fifty two and 48 components in the leaves and fruits, repectively, were identified in Z. schinifolium. (E)-2-hexenal,
, (Z)-ocimene+limonene, estragole, germacrene-d were detected at common components in the leaves and estragole in the fruits of Z. schinjfolium. Regardless of collection sites hexanal, (Z)-3-hexenol, (E)-2-hexenal, n-hexanol were appeared in the leaves while undecanone in the fruits. Thirty and 27 components in the leaves and fruits, respectively, were identified in Z. piperitum.
, 1,8-cineole, citronellal and myrcene, (Z)-ocimene+limonene,
were appeared as common components in the leaves and fruits collected from Baeck-yang-sa and Nae-jang-sa. (Z)-3-hexenol, (E)-2-hexenal,
, citronellal, geranyl acetate were major components in the leaves and fruits from Tong-do-sa.
Growth of Seedling and Germination Characteristics of Acanthopanax koreanum NAKAI
Ko, Han-Jong ; Song, Chang-Khil ; Cho, Nam-Ki ;
Korean Journal of Medicinal Crop Science, volume 11, issue 1, 2003, Pages 46~52
This experiment was conducted to investigate the propagation for mass production of Acanthopanax koreanum NAKAI utilized for health food and medicine material. Germination rate of A. koreanum seeds were 64% when seeds were stored at 15 for 60 days and then were treated with 50 ppm of kinetin and dormancy were broken at 5 for 60 days. Rooting rates of green-wood cuttings treated with IBA 100 ppm, NAA 50 ppm, and IAA 100 ppm were 61.7, 56.7, and 60.0%, respectively. Rooting rates of greenwood cuttings treated with Rooton in scoria + horticulture media and volcanic ash were 76.7 and 66.7%, respectively. Survival rate of seedlings planted on Aug. 10 was highest (96%) under 75% shading net while shoot growth was best under 55% shading net. Survival rate of seedlings planted on May 2 was highest (91%) 55% shading net, and shoot growth was also best 55% under shading net.
Antioxidant, Antimutagenicity and Anticancer Activities of Extracts from Cirsium japonicum var. ussuriense KITAMURA
Lee, Hui-Kyoung ; Kim, Ju-Sung ; Kim, Na-Young ; Park, Sang-Un ; Kim, Myong-Jo ; Yu, Chang-Yeon ;
Korean Journal of Medicinal Crop Science, volume 11, issue 1, 2003, Pages 53~61
Cirsium japonicum var. ussuriense were extracted with methanol and then fractionated with nhexane, EtOAc and BuOH to get active fractions. And their antioxidant and antimicrobial activities in each fraction were determined. Ethyl acetate and butanol fraction of Cirsium japonicum var. ussuriense showed strong antioxidant activities, but hexane fraction did not show any activities. But in the antimicrobial test, Ethyl acetate fraction showed strong antimicrobial activities except to Aspergillus awamori, Asperigillus niger. Especially, Ethyl acetate fraction showed the strongest activities against Bacillus subtilis. And aqueous fraction showed the strongest activities against Cladosporium herbarum, Hypocrea nigricans. This study was performed to determine the antimutagenic and cytotoxic effect of Cirsium japonicum var. ussuriense methanol extract on Salmonella typhimurium TA98, TA100 and cancer cell lines using Ames test and cytotoxicity assay, respectively. Cancer cell lines include human lung carcinoma(A549), human breast adenocarcinoma(MCF-7) and human hepatocellular carcinoma (Hep3B). Futher fractionations with hexane, ethyl acetate, butanol and water from methanol extract of Cirsium japonicum var. ussuriense were performed to obtain effective fraction, methanol extract showed 60.14% inhibition effect on the mutagenesis induced by MNNG against TA100, while 77% and 72.5% inhibition was observed on the mutagenesis induced by 4NQO against TA98 and TA100, respectively. and methanol extract showed 82.25% and 73.7% inhibitory effect on the mutagenesis induced by Trp-P-1 against TA98 and TA100, respectively. methanol extract showed the strongest effect against A549, MCF-7 and Hep3B at the same concentration compared to those of other fration.
Growth enhancement and cytotoxicity of Korean mistletoe fractions on human cell lines
Lee, So-Jin ; Lee, Mi-Kyoung ; Choi, Geun-Pyo ; Yu, Chang-Yeon ; Roh, Seong-Kyu ; Kim, Jong-Dai ; Lee, Hyeon-Yong ; Lee, Jin-Ha ;
Korean Journal of Medicinal Crop Science, volume 11, issue 1, 2003, Pages 62~70
The biological activities on human immune and cancer cell lines of the four kinds of Korean mistletoes (Korean Viscum album, var. coloratum, : Korean Viscum sp. in Quercus acutissima Carr., Korean Viscum sp. in Castanea crenata, Korean Viscum sp. in Betula platyphylla, and Korean Viscum sp. in Salix koreensis) extracts were investigated. The extracts were preparated with ethanol, and fractionated with n-butanol, ethyl acetate, chloroform, hexane, and second distilled water. Cytotoxic potencies of the fractions on human normal lung cell line (HEL 299) showed under 28% in the concentration of 0.5 mg/ml. Growth inhibition effect of the Korean mistletoe extracts on the several human cancer cell lines depends on the concentration of the extracts, and extracting solvent. The hexane, chloroform, and ethyl acetate fractions indicated a strong anticancer activity, but not in aqueous and butanol fractions. Some mistletoe fractions have a different characteristic on the cancer cell lines. Stimulation on the growth of human immuno cell lines(B cell : Raji, T cell: Jurkat) of the extracts were confirmed in the ethyl acetate, chloroform, hexane fractions, but not in aqueous system.
Varietal and Yearly Differences of Lignan Contents in Fruits of Collected Lines of Schizandra chinensis Baillon
Kim, Kwan-Su ; Park, Chun-Gun ; Bang, Jin-Ki ;
Korean Journal of Medicinal Crop Science, volume 11, issue 1, 2003, Pages 71~75
To know the varietal difference and yearly changes of lignan contents in the fruits of collected lines of Schizandra chinensis Baillon, three lignan compounds, schizandrin, gomisin A, and gomisin N, were quantitatively analyzed using High Performance liquid Chromatography. The average contents of schizandrin, gomisin A, and gomisin N, showed 0.78%, 0.25%, and 0.63% in the 6-year-old fruits of 24 lines harvested in 1996, and 0.72%, 0.22%, and 0.63% in the 7-year-old ones of 59 lines harvested in 1997. There were the wide range of coefficient of variation (CV) values, the significant differences by the lines and harvest years, and the significant interaction between line and year for lignan contents. Schizandrin contents during 3 years,
, showed relatively higher amounts more than 0.9% in Chungju 143, Chungju 453, and Chungju 532. Among 23 lines analyzed from 1995 to 1997, Chungju 542, Chungju 547, and Chungju 580 contained continuously higher amounts more than 0.8% of schizandrin and had lower CV values of lignan contents by the harvest years than the other lines. There were a highly significant and negative correlation between fruit weight and lignan contents, and a positive correlation among contents of schizandrin, gomisin A, gomisin N, and total lignan.