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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Medicinal Crop Science
Journal Basic Information
Journal DOI :
The Korean Society of Medicinal Crop Science
Editor in Chief :
Volume & Issues
Volume 11, Issue 5 - Dec 2003
Volume 11, Issue 4 - Nov 2003
Volume 11, Issue 3 - Sep 2003
Volume 11, Issue 2 - Jun 2003
Volume 11, Issue 1 - Mar 2003
Selecting the target year
Characteristics of Morphological and Production from Different Origin of Foeniculum vulgare Mill.
Chung, Hae-Gon ; Kim, Seong-Min ; Nemeth, E. ;
Korean Journal of Medicinal Crop Science, volume 11, issue 4, 2003, Pages 259~263
This study was carried out to get basic information about the morphological and production characteristics of fennel populations different origin under different ecological conditions in Hungary. The Korean population can be registered as a medium high genotype and showed a more accelerated growth characters than Hungarian taxon and the two populations have a medium homogeneity
. In the second year plant height in Hungarian taxon was significantly higher than that of the Korean population. The shooting dynamic of individual plants might be heterogeneous in both taxa and the Korean population was not only shorter in the height but had also less shoots by 16% than the Hungarian one. The Hungarian taxon exceeded the umbel size and numbers to the Korean one (by 3.62 cm in the average) and the variability in the populations was high
. Seed size was proved to be also a discriminative feature between the examined taxa. With small deviations (CV < 2) the Hungarian population produced seeds longer by 38%. Homogeneity in the populations was dependent more on the vegetation years and on the characteristics measured but less on the origins, respectively.
Inhibitory Effects of Methanol Extracts from Korean Medicinal Plants against HIV-1 Protease Activity
Park, Jong-Cheol ; Miyashiro, Hirotsugu ; Hattori, Masao ;
Korean Journal of Medicinal Crop Science, volume 11, issue 4, 2003, Pages 264~267
Korean medicinal plants were screened for their inhibitory activity against HIV-1 protease. The inhibitory activity of protease was determined by incubating the extracts in reaction mixtures containing protease and substrate
to perform proteolytic cleavage reactions. In this study the twenty six extracts from medicinal plants were investigated. Of the extracts tested, the extracts from the stem of Morus alba. exhibited the strongest activity with inhibition of 81% at a concentration of
. The extracts of the flower of Saxjfraga stolonifera, and stems of Euonymus japonica and Castanea crenata showed appreciable inhibitory activity (>50%) against HIV-1 protease at same concentration.
Discrimination of Species Specific DNA Markers Using RAPD and AFLP Analysis between Atractylodes japonica Koidz. and Atractylodes macrocephala Koidz.
Bang, Kyong-Hwan ; Park, Chun-Geon ; Jin, Dong-Chun ; Kim, Hong-Sig ; Park, Hee-Woon ; Park, Chung-Heon ; Seong, Nak-Sul ;
Korean Journal of Medicinal Crop Science, volume 11, issue 4, 2003, Pages 268~273
To identify the variation of the RAPD patterns between two Atractylodes species, 52 kinds of random primers were applied to each eight of A japonica and A. macrocephala genomic DNA. Ten primers of 52 primers could be used to discriminate between the species and 18 polymorphisms among 67 scored DNA fragments (18 fragments are specific for A. japonica and A. macrocephala) were generated using these primers, 26.9% of which were polymorphic. RAPD data from the 10 primers was used for cluster analysis. The cluster analysis of RAPD markers showed that the two groups are genetically distinct. On the other hand, to identify the variation of the AFLP patterns and select the species specific AFLP markers, eight combinations of EcoRI/MseI primers were applied to the bulked A. japonica and A. macrocephala genomic DNA. Consequently, three combinations of EcoRI/MseI primers (EcoRI /Mse I ; AAC/CTA, AAC/CAA, AAG/CTA) used in this study revealed 176 reliable AFLP markers, 42.0% of which were polymorphic. 74 polymorphisms out of 176 scored DNA fragments were enough to clearly discriminate between two Atractylodes species.
Identification of the Major Volatile Components from Different Plant Organs of Foeniculum vulgare Mill.
Chung, Hae-Gon ; Bang, Jin-Ki ; Kim, Geum-Soog ; Seong, Nak-Sul ; Kim, Seong-Min ;
Korean Journal of Medicinal Crop Science, volume 11, issue 4, 2003, Pages 274~278
The various plant organs of fennel (Foeniculum vulgare Mill.) were investigated to identify their volatile components using Dynamic Headspace (purge & trap). They showed slight differences concerning the volatile components both qualitatively and quantitatively. Results revealed that trans-anethole (12.65%) was the major compound in the leaf. The highest compound was
(28.78%), and trans-anethole (7.90%) was highly detected in the stem. The maximum values were 5.64, 4.59, 1.58, 1.51, and 1.04% for
, 1,8-cineol and fenchone, respectively in the flower. However, very little trans-anethole was detected (0.27%) in the flower. From these results, it was suggested that the major components were different depending on the plant organs. However it was demonstrated that the related plant organs like flower-fruit and leaf-stem contained the similar components.
Lignans from the Root of Acanthopanax chiisanensis Nakai
Lee, Sang-Hyun ; Shin, Kuk-Hyun ; Lee, Sang-Chul ; Cho, Seon-Haeng ;
Korean Journal of Medicinal Crop Science, volume 11, issue 4, 2003, Pages 279~283
Five lignans were isolated from the chloroform fraction of Acanthopanax chiisanensis Nakai root by open column chromatography. Their structures were elucidated as (-)-sesamin (1), helioxanthin (2), savinin (3), taiwanin C (4), and 3-(3",4"-dimethoxybenzyl)-2-(3',4'-methylenedioxybenzyl) butyrolactone (5) by spectral analysis. Among them, compounds 2, 3, 4, and 5 were isolated for the first time from this plant.
Changes of Rutin Content and Photosynthesis Rate of Korean Buckwheat Cultivars under Various Environmental Stresses
Yoon, Byeong-Sung ; Kwun, Hyok-Oun ; Shin, Sang-Eun ; Jin, Cheng-Wu ; Yu, Chang-Yeon ; Cho, Dong-Ha ;
Korean Journal of Medicinal Crop Science, volume 11, issue 4, 2003, Pages 284~288
This study was conducted to classify photosynthesis rate and changes of rutin content of Korean buckwheat (cv. Chunchon-jaerae and Yangjeul-memil) treated with salinity, UV-C and low temperature. In case of cv. Chunchon-jaerae and Yangjeul-memil, according to the salt stress, transpiration rate, stomatal conductance and photosynthesis rate were decreased. Both cultivars also showed decrease of transpiration rate and photosynthesis rate under the UV-C. Rutin contents within leaf and stem of cv. Yangjeul-memil were decreased when NaCl concentration was high. Rutin contents within leaf and stem of cv. Yangjeul-memil were generally decreased when the time laps under the UV-C stress. Rutin contents within leaf and stem of cv. Chunchon-jaerae was also generally decreased when the time laps under the low temperature stress.
Agrobacterium Mediated Transformation of Rehmannia glutinosa L. with Glutathione S-Transferase Gene (Gh-5)
Lim, Jung-Dae ; Sung, Eun-Soo ; Yang, Deok-Chun ; Yun, Song-Joong ; Chung, Ill-Min ; Kim, Myong-Jo ; Yu, Chang-Yeon ;
Korean Journal of Medicinal Crop Science, volume 11, issue 4, 2003, Pages 289~297
Using Agrobacterium-me야ated transformation method the auxin-regulated cotton GST (Gh-5) constructs were used to transform Rehmannia glutinosa L. The PCR analysis was conducted to verify transgenicity. Based on the PCR analysis, there was verified that the 988 bp DNA band had showed in transgenic plant genomes in PCR anaJysis using Gh5-1 and Gh5-2 primers. The effects of cocultivation with Agrobacterium tumefaciens, regeneration and selection conditions on the transformation efficiency of Chinese foxglove (Rehmannia glutinosa L.) were investigated. Factors such as cocultivation period, use of acetosyringone, postcultivation in darkness, and different kanamycin concentrations for selection were assessed. In vitro regeneration, the number of leaves, shoot lengths and numbers on MS medium were superior to on B5 and WPM medium, and the shoot formation rate was highest level of 95% in cultured base part containing leaf stalk. Addition of acetosyringone at concentration of
to cocultivation medium and 3-day of cocultivation improved transformation frequencies. Exposure of explants to darkness for 4 weeks on selection medium resulted in further increased the regeneration frequency of transgenic shoots. In PCR analysis, the amplified fragments of Gh5 gene were detected (988 bp), and GST-expressing transgenic R. glutinosa L. plants had approximately three-fold higher activity in leaf extracts compared with control plant.
Methanol Extract of Polygalae Radix Protects Excitotoxicity in Cultured Neuronal Cells
Ban, Ju-Yeon ; Lee, Hyun-Joo ; Lee, Soo-Bae ; Lee, Young-Jong ; Seong, Nak-Sul ; Song, Kyung-Sik ; Bae, Ki-Whan ; Seong, Yeon-Hee ;
Korean Journal of Medicinal Crop Science, volume 11, issue 4, 2003, Pages 298~305
Polygalae Radix (PR) from Polygala tenuifolia. (Polygalaceae) is traditionally used in China and Korea, since this herb has a sedative, antiinflammatory, and antibacterial agent. To extend pharmacological actions of PR in the CNS on the basis of its CNS inhibitory effect, the present study examined whether PR has the neuroprotective action against kainic acid (KA) -induced cell death in primarily cultured rat cerebellar granule neurons. PR, over a concentration range of 0.05 to
-induced neuronal cell death, which was measured by a trypan blue exclusion test and a 3-[4,5-dimethylthiazol-2-y1]-2,5-diphenyl-tetrazolium bromide (MTT) assay. PR
inhibited glutamate release into medium induced by KA
, which was measured by HPLC. Pretreatment of PR
-induced elevation of cytosolic calcium concentration
which was measured by a fluorescent dye, Fura 2-AM, and generation of reactive oxygen species (ROS). These results suggest that PR prevents KA-induced neuronal cell damage in vitro.
Phytochemical Constituents of Acanthopanax senticosus (Rupr. & Maxim.) Harms Stem
Ryu, Ji-Young ; Son, Dong-Wook ; Kang, Jung-Il ; Lee, Sang-Yun ; Kim, Hyun-Su ; Shin, Kuk-Hyun ; Lee, Sang-Hyun ;
Korean Journal of Medicinal Crop Science, volume 11, issue 4, 2003, Pages 306~310
Five constituents were isolated from the stem of Acanthopanax senticosus. Their structures were elucidated as (-)-sesamin (1), iso-fraxidin (2), 5-hydroxymethylfurfural (3), syringin (4) and acanthoside D (5) by spectral analysis. Among these compounds, 5-hydroxymethylfurfural (3) was isolated for the first time from this plant.
Comparative Cytogenetic Characteristics and Physical Mapping of the 17S and 5S Ribosomal DNAs between Atractylodes japonica Koidz. and Atractylodes macrocephala Koidz.
Bang, Kyong-Hwan ; Koo, Dal-Hoe ; Kim, Hong-Sig ; Song, Beom-Heon ; Cho, Yong-Gu ; Cho, Joon-Hyeong ; Bang, Jae-Wook ;
Korean Journal of Medicinal Crop Science, volume 11, issue 4, 2003, Pages 311~315
This study was carried out to compare chromosomal characteristics between Atractylodes japonica and A macrocephala. Cytogenetic analysis was conducted based on karyotype analysis and physical mapping using fluorescence in situ hybridization. As a result of karyotype analysis by feulgen staining, somatic chromosome numbers of A. japonica and A. macrocephala were 2n=24. The length. of the mitotic metaphase chromosomes of A. japonica ranged from
with a total length. of
and the homologous chromosome complement comprised six metacentrics, five submetacentrics and one subtelocentrics. On the other hand, the length of the mitotic metaphase chromosomes of A. macrocephala ranged from
with a total length of
and the homologous chromosome complement comprised seven metacentrics and five submetacentrics. The total length of A. japonica chromosomes was shorter than that of A. macrocephala, but A. japonica had one subtelocentrics (chromosomes 4) different from A. macrocepha1a. chromosomes. The F1SH technique using 17S and 5S rDNA was applied to metaphase chromosomes. The signals for 17S rDNA were detected on the telomeric regions of chromosomes 4 and 5 in both A japonica and A. macrocephala. The 5S rDNA signal was found in the short arm of chromosome 1.
An Efficient In vitro Propagation of Zanthoxylum piperitum DC.
Hwang, Sung-Jin ; Hwang, Baik ;
Korean Journal of Medicinal Crop Science, volume 11, issue 4, 2003, Pages 316~320
A protocol is described for rapid multiplication of Zanthoxylum piperitum DC. (Rutaceae), an important aromatic and medicinal plant, through shoot-tip explant cultures. Murashige and Skoog (MS) medium supplemented with various concentrations of N-6-benzyladenine (BA), N-6-benzylaminopurine (BAP) and thidiazuron (TDZ), in single or in combination with
acid (NAA), was used to determine the rate of shoot proliferation. N-6-benzyladenine (BA) used at 0.5mg/l, was the most effective in initiating multiple shoot proliferation at the rate of 23 microshoots per shoot-tip explants after 40 days of culture. Shoot multiplication increased 1.2-fold in each successive subculture. Induction of rooting (98%) was achieved by transferring the shoots to the same basal medium containing 2 mg/l indole-3-butyric acid (IBA). Plantlets went through a hardening phase in a controlled growth chamber, prior to in vivo transfer. These results represented that possible application for the mass production of plantlets through in vitro culture system of Zanthoxylum piperitum DC.