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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Medicinal Crop Science
Journal Basic Information
Journal DOI :
The Korean Society of Medicinal Crop Science
Editor in Chief :
Volume & Issues
Volume 11, Issue 5 - Dec 2003
Volume 11, Issue 4 - Nov 2003
Volume 11, Issue 3 - Sep 2003
Volume 11, Issue 2 - Jun 2003
Volume 11, Issue 1 - Mar 2003
Selecting the target year
A Proposed Model for Prosowing Seed Treatments to Promote Germination and Seedling Emergence
Kang, Jin-Ho ; Yoon, Soo-Young ;
Korean Journal of Medicinal Crop Science, volume 11, issue 5, 2003, Pages 321~328
Various presowing seed treatments have been used to enhance the rates of germination and afterward seedling emergence. Seeds treated by them occasionally have not shown the same rate in indoor and field. The presowing treatments considering germination mechanism and factors affecting germination should be totally included in indoor experiments so that the results drawn could be reproduced in the field. Seed drying after the treatments and field conditions after sowing especially able to show Phytochromemediated responses related to light treatment must be determined prior to force the treatments to seeds. After set up these prerequisites to promote the rates of germination and emergence, many seed treatments proposed so far must be fixed how to be done; alone, sequential and simultaneous.
Biological Activities of Roasted Chicory Root
Park, Chae-Kyu ; Jeon, Byeong-Seon ; Shim, Ki-Hwan ;
Korean Journal of Medicinal Crop Science, volume 11, issue 5, 2003, Pages 329~335
This study was carried out to investigate physiological activities of chicory root (Cichorium intybus L. var. sativus). The anti-hepatotoxic activity of roasted chicory was studied using primary cultured rat hepatocytes where cytotoxicity was induced by galactosamine. The water extract of roasted chicory did not induced of cytotoxicity in primary cultured rat hepatocytes. Treatment with 5 mM galactosamin for 5.0 hr showed maximum increase in activity of lactic dehydrogenase (LDH) released in the medium. The water extract of roasted chicory inhibited significantly and dose-dependently the release of LDH activity increased by galactosamine-induced cytotoxity. The antidiabetic activity of water extract of roasted chicory was examined in streptozotocin induced diabetic rats. The increased blood glucose level in the streptozotocin induced diabetic rats was significantly decreased by the administration of chicory extract (800 mg/kg). Chicory water extract (800 mg/kg) prevented weight losses in streptozotocin induced diabetic rats. The antimutagenic activities of chicory water extract were tested using Salmonella thyphimurium YG 1024 as tester strains and 2-aminofluorence as a potent carcinogen in the presence of S-9 mix. No mutagenic activities of the water extracts of roasted chicory were observed on all the tested strains at dose
per plate. Water extract of roasted chicory did not inhibit the mutagencities of Salmonella thyphimurium YG 1024 induced by 2-aminofluorene.
Somatic Embryogenesis and Organogenesis via Callus Culture from Hydrocotyl maritima Honda
Kim, Ok-Tae ; Kim, Min-Young ; Kim, Kwang-Soo ; Ahn, Jun-Cheul ; Hwang, Baik ;
Korean Journal of Medicinal Crop Science, volume 11, issue 5, 2003, Pages 336~339
Hydrocotyle maritima Honda used as medicinal plants for a hemostatic agent was investigated for in vitro regeneration. The petiole explants of H. maritima were cultured on callus induction medium containing growth regulators (
, NAA and
2,4-D) either in single or in combination with
BA for 6 weeks. Although single treatments of 2,4-D or NAA resulted in callus formation, the best results were combination of
, 2,4-D and
, NAA and
, BA, respectively. The highest number of shoot (12 shoots per callus) was achieved with
, Kinetin. Also, when pieces of embryogenic callus induced on the medium supplemented with
, 2,4-D and
, BA were subcultured on hormone-free medium, somatic embryos were differentiated and developed further into welldeveloped plants.
Seedling Growth of Ginseng (Panax ginseng C. A. Meyer) Affected by Composition of its Bed Soil and Height of Front Piller
Ahn, Mun-Sub ; Kang, An-Seok ; Kim, Se-Won ; Lee, Se-Jong ;
Korean Journal of Medicinal Crop Science, volume 11, issue 5, 2003, Pages 340~346
This study was carried out to improve cultivation techniques by low cost and labour saving in ginseng (Panax ginseng C. A. Meyer) seedling production, by elucidating proper ratio virgin soil and organic fertilizer, suitable height of front piller. The obtained results are as follows ; The optimal ratio of white decomposition of virgin soil and organic fertilizer was 10:1 for good yield of standard seedling, The fittest height of front piller was 150 cm because of both good seedling growth and yield. The cost for production of seedling of ginseng could be reduced by both optimal ratio of virgin soil, organic fertilizer and selection of front piller height.
Dehisced Seed Germination and Seedling Growth Affected by Chilling Period in Eleutherococcus senticosus Maxim.
Li, Cheng Hao ; Lim, Jung-Dae ; Kim, Myong-Jo ; Heo, Kwon ; Yu, Chang-Yeon ;
Korean Journal of Medicinal Crop Science, volume 11, issue 5, 2003, Pages 347~351
This experiment was carried out to study the optimal chilling period for breaking the physiological dormancy of dehisced E. senticosus seeds and to investigate the critical seeding date in Kangwondo. Storage at
for 85 days was most effective in breaking dormancy of dehisced seeds, but didn't germinated synchronously. Only 28.1% of undehisced seeds germinated after 125 days of low temperature storage. For improving seedling survival rate in the field, 50% shading was more effective than 30% shading. Delayed seeding date resulted in decreased seedling fresh weight, dry weight, leaf area and root length, but seeding date has no significant affecte on stem length.
Comparison of Volatile Compounds in Plant Parts of Angelica gigas Nakai and A. acutiloba Kitagawa
Cho, Min-Gu ; Bang, Jin-Ki ; Chae, Young-Am ;
Korean Journal of Medicinal Crop Science, volume 11, issue 5, 2003, Pages 352~357
Volatile flavor compounds Angelica gigas Nakai and Angelica acutiloba Kitagawa were extracted by SDE (simultaneous steam distillation & extraction) using the mixture of n-pentane and diethylether (1:1, v/v) as an extract solvent and analyzed by GC-FID and GC-MS. The amount of essential oils of top part and root in Angelica gigas were obtained in 0.063% (v/w) and 0.389% (v/w) yields as a fresh weight base, respectively. The main compounds in top parts and the root were identified as nonane (7.51% and 24.49%, respectively),
(14.64% and 31.75%), limonene+
(14.01% and 9.66%),
(7.85% and 1.20%), germacrene-d (5.85% and 0.22%), (E,E)-
(6.05% and 1.40%),
(5.26% and 1.84%). Although these compounds were present in both parts. The results showed large differences in. the concentrations of them much varied. The amount of essential oils stem and leaf obtained (0.068% and 0.127% in A. gigas) and (0.153% and 0.243% in A. acutiloba) yields as a fresh weight base, respectively. More than 18 and 32 components in stem and leaf have been identified, which of main components in A. gigas were
, myrcene, limonene, germacrene-d, eudesmol and butylphthalide, but germacrene-d and butylphthalide contents were also different in stem and leaf. And more than 21 and 32 components in A. acutiloba were
and butylphthalide. Volatile compounds were very different in both species.
Selection of Optimal Biotic Elicitor on Tropane Alkaloid Production of Hairy Roots in Scopolia parviflora Nakai
Jung, Hee-Young ; Kang, Seung-Mi ; Kang, Young-Min ; Kim, Yong-Duk ; Yang, Jae-Kyung ; Chung, Young-Gwan ; Choi, Myung-Suk ;
Korean Journal of Medicinal Crop Science, volume 11, issue 5, 2003, Pages 358~363
ScopoIamine and hyoscyamine which belong to tropane alkaloids are the pharmaceutically valuable anticholinergic drugs. In order to increase the productivities, the effects of elicitation were investigated during hairy root cultures of Scopolia. parviflora. Biotic elicitors originated from 3 fungi and 1 yeast were prepared as homogenate and supernatant and added to 3-week-old cultures. Both of homogenate and supernatant of Candida albicans elicitors increased the scopolamine production. The production of hyoscyamine was enhanced by homogenate of Fusarium solani and supernatant of C. albicans. Most of the other fungal elicitors were also improved on the tropane alkaloid production compared to non-treatment. Among the elicitors tested, C. albicans was proved the optimal biotic elicitor on tropane alkaloids production. These results will be served mass production of tropane alkaloids by large-scale production.
Extraction and Biological Activities of Essential Oil from Thuja occidental Leaves
Seo, Weon-Taek ; Yang, Jae-Kyung ; Kang, Byung-Kuk ; Park, Woo-Jin ; Hong, Seong-Cheol ; Kang, Young-Min ; Jung, Hee-Young ; Kim, Yong-Duck ; Kang, Seung-Mi ; Kim, Seon-Won ; Choi, Myung-Suk ;
Korean Journal of Medicinal Crop Science, volume 11, issue 5, 2003, Pages 364~370
Essential oil of Thuja occidental leaves was extracted by steam distillation method, and chemical composition of essential oil was analyzed by GC-MS. The essential oil was tested to antimicrorbiaI activity against 28 test microorganisms and antioxident assay through DPPH free radical scaveging test and insecticidal activity by mosquito insecticide assay. The major constituents in the essential oil of T. occidental were monoterpene as d-isothujone,
, camphor, L-fenchone, and hornyl acetate. The essential oil have broad antimicrobiaI activity, which showed strong antimicrobiaI activity for Streptococcus pyogenes as bacteria, Alternaria alternata, Aspergillus niger, Aspergillus oryzae, Saccharomyces cerevisiae, and Streptococcus mutans as fungi. The essential oil also showed DPPH free radical scavenging ability at concentration of
. In spraying and fumigation tests, the essential oil had strong insecticidal activity against mosquito.
Effects of Pig Manure Application on Nitrogen Uptake, Yield and Active Components of Chrysanthemum boreale M.
Lee, Kyung-Dong ; Yang, Min-Suk ;
Korean Journal of Medicinal Crop Science, volume 11, issue 5, 2003, Pages 371~376
To develop an efficient cultivation system to increase the productivity and the high quality of Chrysanthemum boreale M., the effects of pig manure (PM) application on the yield and the effective component were investigated in the pot scale (1/2000a scale). PM applied at the equivalent of six rates (with rate of 0, 2000, 4000, 6000, 8000, and 12000 kg
). Maximum plant biomass yield was achieved at 9510 kg
and at 9940 kg
for flower biomass. Nitrogen recovery efficiency was more than 42% for all nitrogen treatments and reached 66.6% at 4000 kg
was the most abundant amino acid in the flower of C. boreale M. and the contents of amino acids increased with increasing PM application rate. Contents of cumambrin A. a sesquiterpene compound known to reduce blood-pressure, decreased with increasing PM application. The highly negative correlation was found (
= -0.723, P<0.01) between content of cumambrin A and PM application. However, the amount of cumambrin A in flowers increased as PM rate increased, because of increasing flower yield. In conclusion, PM fertilization increases yields and enhances overall quality.
Analysis of Diversity of Panax ginseng Collected in Korea by RAPD Technique
Seo, Sang-Deog ; Yuk, Jin-Ah ; Cha, Sun-Kyung ; Kim, Hyun-Ho ; Seong, Bong-Jae ; Kim, Sun-Ick ; Choi, Jae-Eul ;
Korean Journal of Medicinal Crop Science, volume 11, issue 5, 2003, Pages 377~384
Genetic differences among nine land races of Korean ginseng (Panax ginseng C. A. Meyer) were examined using RAPD markers. Land races of Korean ginseng were collected from nine regions in Korea: Cheongwon, Guesan, Geumsan, Namwon, Pochun, Yangju, Yeoncheon, Yeongju. Out of 48 RAPD primers tested, 5 primers (OPA 7, OPA 13, URP 2, URP 3 and UBC 3) produced remarkable bands which showing polymorphisms among evaluated collections. Lower levels of genetic diversity were in detected same land races than among other land races. Genetic differences within and among land races indicate heterogeneity. These results indicate that cultivated ginseng in Korea is heterogeneous. Genetic similarity matrices of RAPD profiles were generated via coefficients of variation and the data were processed by the cluster analysis (UPGMA). When 90 collections were evaluated using selected 5 primers, those were clustered to 5 and 3 subgroups. These differences in genetic variation between land races of Korean ginseng implied the potential source for further breeding of Korean ginseng.
Effects of Some Herbal Extracts on Aflatoxin
Production from Aspergillus parasiticus
Chung, Sang-Jin ;
Korean Journal of Medicinal Crop Science, volume 11, issue 5, 2003, Pages 385~391
The influences of the extracts from Cinnamomi Cortex, Eucommiae Cortex, Puerariae Radix, Lycii Fructus, Zizyphi Fructus, Schisandrae Fructus, Mume Fructus, Chaenomelis Fructus on mycelial growth and aflatoxin
production from Aspergillus parasiticus were analyzed. The pH of the culture media were reduced to below pH 4 by all the herbal extracts after 3 days incubation. However, the pH of the culture media increased above pH 6 after 6 days incubation using the extracts from Cinnamomi Cortex, Eucommiae Cortex, Puerariae Radix and Lycii Fructus. The mycelial growth of A. parasiticus was increased over the amount of the control. Puerariae Radix produced the largest amount of mycelial growth and Chaenomelis Fructus produced the smallest amount of mycelial growth. The productions of aflatoxin
from A. parasticus culture were increased by the extracts of Puerariae Radix and Zizyphi Fructus, while inhibited by the extracts of Cinnamomi Cortex, Eucommiae Cortex, Lycii Fructus, Schisandrae Fructus, Mume Fructus and Chaenomelis Fructus. In particular, the extracts of Cinnamomi Cortex, Lycii Fructus and Schisandrae Fructus almost inhibited the production of aflatoxin
. The production of the total protein from Cinnamomi Cortex, which produced much less aflatoxin
, and Puerariae Radix, which produced a great deal of aflatoxin
from A parasticus were slightly higher than the production of the total protein of the control medium.
Plant Growth and Bolting Affected by Transplanting Time in Angelica gigas
Yu, Hong-Seob ; Jo, Jae-Seong ; Park, Chung-Heon ; Park, Chun-Geon ; Sung, Jung-Sook ; Park, Hee-Woon ; Seong, Nak-Sul ; Jin, Dong Chun ;
Korean Journal of Medicinal Crop Science, volume 11, issue 5, 2003, Pages 392~396
This study was carried out to determine plant growth and bolting characteristics of Angelica gigas. Three varieties and/or lines Manchu bolting resistant variety, AGJ90-1 medium type line, and bolting Jinbu local were used for field trial in Suwon. FIve different transplanting times were applied on March 25, April 5, April 15, April 25, and May 5th. Survival rate was decreased by the delayed transplantation and it affected after April 25th respectively. Plant growth by the early transplantation showed the best growth during the beginning stage and recognized signifiquence but not by the late transplantation. Bolting rate revealed severe different by the variety and line, it ranged from
of AGJ90-1, and
of Jinbu local clearly. By the treatment of transplantation time bolting rate was different, early plantation showed high bolting rate compare to the late plantation from the all of tested variety and lines. Bolting response according to the transplanting time, bolting resistant variety showed insensibility and boling line showed sensitive response. In Manchu root yield was best when planted on April 5th as 389 kg /10a, in case of AGJ90-1 was determined 377 kg/10a when planted on April 15th, and Jinbu local root yield was showed 133 kg/10a planted on April 25.
Shoot Regeneration from Leaf Explants of Digitalis purpurea L.
Hwang, Sung-Jin ; Lee, Hye-Jung ; Hwang, Baik ;
Korean Journal of Medicinal Crop Science, volume 11, issue 5, 2003, Pages 397~401
Digitalis purpurea L. is a medicinal herb and have been used to congestive heart failure, mycocardial infarction, edema, angina etc. A protocol has been developed for in vitro propagation of adventitious shoot buds directly from leaf segments of D. purpurea Leaf explants of D. purpurea directly formed shoot buds when cultured on a MS medium supplemented with
IAA for 5 weeks. Adventitious shoots were multiplied by subculturing on the
medium and shoot elongation was developed by subculturing on the WPM medium. Root formation from the shoot regenerated was achieved on MS basal medium containing 1 mg/ IBA.
Karyotype Analysis and Physical Mapping of rDNAs in Bupleurum longeradiatum
Koo, Dal-Hoe ; Seong, Nak-Sul ; Seong, Jong-Suk ; Bang, Kyong-Hwan ; Bang, Jae-Wook ;
Korean Journal of Medicinal Crop Science, volume 11, issue 5, 2003, Pages 402~407
Karyotype analysis and chromosomal localization of 5S and 45S rDNAs using multi-color fluorescence in situ hybridization (McFISH) technique were carried out in Bupleurum longeradiatum. Somatic metaphase chromosome number was 2n=12. Karyotype was composed of three pairs of metacentrics (No.3, 4 and 6) and three pairs of submetacentrics (No. 1, 2 and 5). The length of somatic prometaphase chromosomes ranges from 2.55 to
with total length of
. In FISH experiment, one pair of 5S rDNA signals was detected on the pericentromeric region of chromosome 4 and one pair of 45S rDNA signals was detected on the telomeric region of chromosome 2.