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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Medicinal Crop Science
Journal Basic Information
Journal DOI :
The Korean Society of Medicinal Crop Science
Editor in Chief :
Volume & Issues
Volume 12, Issue 6 - Dec 2004
Volume 12, Issue 5 - Nov 2004
Volume 12, Issue 4 - Sep 2004
Volume 12, Issue 3 - Jun 2004
Volume 12, Issue 2 - May 2004
Volume 12, Issue 1 - Mar 2004
Selecting the target year
Isolation of Antimicrobial Compounds from Salvia miltiorrhiza Bunge
Han, Wan-Soo ;
Korean Journal of Medicinal Crop Science, volume 12, issue 3, 2004, Pages 179~182
Bioassay-directed fractionation of the dried roots of Salvia miltiorrhiza led to the isolation of abietane tanshinones, cryptotanshinone and dibydrotanshinone I. Their structures were elucidated using
, UV, IR and mass spectral analyses. These compounds exhibited a moderate antimicrobial activities against Staphylococcus epidemidis, Staphylococcus aureus, and Staphylococcus pyogene.
Major Components of Teas Manufactured with Leaf and Flower of Korean Native Camellia japonica L.
Cha, Young-Ju ; Lee, Jang-Won ; Kim, Ju-Hee ; Park, Min-Hee ; Lee, Sook-Young ;
Korean Journal of Medicinal Crop Science, volume 12, issue 3, 2004, Pages 183~190
The major compositions of leaf tea and flower tea were investigated to develope as a new functional tea using Korean native Camellia japonica L. Most of leaf teas, except flower tea, were considered as good materials with basic conditions for tea manufacture because water content was below 6%. Crude protein was the greatest component in roasted young leaf tea (RYLT), crude fats in roasted mature leaf tea (RMLT) and ashes in fermented young leaf tea (FYLT). Caffein were present as the highest amount (5.18%) in steamed mature leaf tea (SMLT), showing less amount than green tea. Catechin were contained as the highest amount in all kinds of teas, especially FYLT was the highest (9.57%). Tannin, which highly related with tea quality including astringent taste, color and perfume, were present as the highest amount in FYLT. Vitamin C was highly detected in the tea from flowers (22.7 mg/l00 g) rather than in the tea from leaves. The content of theanine were found in flower tea by 1,074 mg/l00 g, and had about twofold of FYLT and RYLT. Among free amino acids, glutamic acid and aspartic acid were higher detected in SMLT and RMLT while asparagine was present as higher amounts in RYLT and FYLT, expecting these components can improve tea taste. Nucleic acids and their derivatives including GMP, hypoxanthine and AMP were detected as the higher amounts by 7.86, 8.57, and
, respectively, however IMP content was even reduced by all manufacturing processes. In all kinds of tea, sugars such as glucose, fructose, sucrose and maltose were detected, specially glucose and fructose were found as highest amount in RFT by 65.5 and 59.6 nmol/0.1 mg, respectively.
Comparision of SOD Activity and Phenolic Compound Contents in Various Korean Medicinal Plants
Lim, Jung-Dae ; Yu, Chang-Yeon ; Kim, Myong-Jo ; Yun, Song-Joong ; Lee, Sun-Ju ; Kim, Na-Young ; Chung, Ill-Min ;
Korean Journal of Medicinal Crop Science, volume 12, issue 3, 2004, Pages 191~202
82 species of Korean medicinal plants were measured to their SOD (superoxide dismutase) activity, total phenol and individual phenol compounds. The SOD activity of medicinal plants was varied from 3.67% to 48.3%, and especially, 4 species including Polygala tenuifolia Willd. showed activity over 40% higher than other species. The 16 individual phenol compounds concentrations have a severe variation in medicinal plants. Codonopsis lanceolata is the highest concentration
, and 10 species including Glycyrrhiza uralensis Fisch were showed to concentrations more than
. And also, salicylic acid was generally found in many plants, but trans-cinnamic acid, naringenin and kampferol was little found in plants. The total phenol by Folin-Dennis methods showed a respectively variation
in 82 medicinal plants, and 7 species including Astragalus membranaceus showed low total phenol concentrations less than
. In conclusion, we expect that selecting medicinal plants with high biological activity might develop to chemicals related to antioxidants, as well as these become most popular substance at many industrial materials, and suggest to more researchs in future.
Identification of Salidroside from Rhodiola sachalinensis A. Bor. and its Production through Cell Suspension Culture
Kim, Soo-Jung ; Kim, Kwang-Soo ; Hwang, Sung-Jin ; Chon, Sang-Uk ; Kim, Young-Ho ; Ahn, Jun-Cheul ; Hwang, Baik ;
Korean Journal of Medicinal Crop Science, volume 12, issue 3, 2004, Pages 203~208
Salidroside was isolated and purified from R. sachalinensis A. Bor. roots. Purified salidroside was obtained from repeated silicagel column chromatography and preparative HPLC, and identified by
COSY spectra analyzer. Callus induction and cell suspension from R. sachalinensis leaf segments were established on 1/2MS solid medium and in
liquid medium containing 0.5 mg/l NAA and 1 mg/l, BA in the dark condition, respectively. The contents of salidroside for suspension culture were ranging from 0.12% to 0.41% in comparison with 0.17% for natural roots.
Embryological Characteristics on Seed Sterility of Ligusticum chuanxiong Hoit.
Park, Chung-Heon ; Lee, Man-Sang ; Namkoong, Seung-Bak ; Yu, Hong-Seob ; Park, Hee-Woon ;
Korean Journal of Medicinal Crop Science, volume 12, issue 3, 2004, Pages 209~213
Ligusticum chuanxiong is receiving much attentions as one of the important medicinal crops with the increasement of the crude drug demands. This study was conducted to obtain the basic informations of breeding of Ligusticum chuanxiong. Embryological characteristics were examined to elucidate the process of male and female gametophytic development and fertilization. Meiosis and nucleus division of megaspore and microspore were proceeded normally. With regard to the formation of female gametophyte, only a half of female gametophyte developed to normal egg apparatus. While another 50% showed abnormal egg apparatus with poly-nuclei or non-nuclei ovule. The pollens developed from the micros pore were formed more than 90 % of normal pollen. It was difficult to observe fertilization because ovule tissue was very compact and cell was extremely tiny, but could be easely observed proembryo and embryo formation. Only 30 percent developed into proembryo and subsequently into embryo, and the others were degenerated.
Analysis of Genetic Relationships of Korean Native Polygonatum spp.
Jang, Kye-Hyun ; Song, Geun-Woo ; Chung, Jong-Il ; Kang, Jin-Ho ;
Korean Journal of Medicinal Crop Science, volume 12, issue 3, 2004, Pages 214~218
Germplasms having valuable characters are increasjngly important for modern breeding programs. This study was conducted to obtain the basic data for effective use of genetic resources of Polygonatum. The relationship of seven Polygonatum species collected widely in Korea was analyzed by RAPD markers. Total number of alleles amplified by nineteen random primers were 114 to 157, and variation in number of alleles was also diverse among seven species examined. The seven species were divided into two groups; one was of Polygonatum stenophyllum and Polygonatum humile. the other was of Polygonatum inflatum, Polygonatum lasianthum, Polygonatum odoratum var. pluriflorum (1), (2), and for variegatum.
Growth and Yield Characteristics by Tillering Sequence in Coix lachryma-jobi L. var. mayuen Stapf
Yi, Eun-Sub ; Yoon, Seong-Tak ; Park, Kyeong-Yeol ;
Korean Journal of Medicinal Crop Science, volume 12, issue 3, 2004, Pages 219~225
This study was conducted to evaluate growth and yield characteristics and the contribution to the yield according to the emergent sequence of tiller under two planting density
in Coix lachryma-jobi L. var. mayuen Stapf. The average culm length and number of rachillas of
planting density were 20 cm shorter and 4.7 higher, respectively, whereas seed setting position of
planting density was 1.7 nodes lower compared with
planting density. Culm length and culm diameter of each tiller became shorter and thinner as the tiller emerged later. Length of rachillas was longer, number of grains was higher and ripening rate was also higher in
planting density than those of
planting density, whereas 100 grain weight in
planting density was lower by 0.5 g compared with
planting density. Length of rachillas and number of grains according to the emergent sequence of tiller were shown shorter and lower as the tiller emerged later. The highest number of bract was obtained from the main culm in both planting densities. 100 grain weight according to the emergent sequence of tiller became lower as the tiller emerged later in
planting density, but there was no clear tendency in
planting density. Among 8 tillers, 5th tiller and 4th tiller of main culm contributed 84.4% to the yield in
planting density, while it was 84.1% in
planting density. Analysis of path coefficient to grain yield showed that direct effect was the highest in the number of grain per tiller followed by in the 100 grain weight and in ripening rate.
Growth and Yield of Atractylodes japonica Koidz. Affected by Mulching Methods
Park, Jeong-Min ; Kang, Jin-Ho ; Jeong, Eun-Ho ; Song, Gewn-Woo ;
Korean Journal of Medicinal Crop Science, volume 12, issue 3, 2004, Pages 226~230
Plant growth should be affected by mulching method. The study was done to examine the effect of different mulching methods on growth and yield of Atractylodes japonica Koidz. The experiment was done by six different mulching methods; no mulching, rice straw, transparent polyethylene (PE), black PE, two combined treatments of rice hull and transparent PE or black PE. Green shoot and related characters, growth and yield of early and late stages were measured on May 8, June 8 and October 26, respectively. Green shoot fresh weight was the highest in transparent PE mulching, while number of leaves, shoot and stem lengths were the greatest in rice straw mulching of the methods. In its early stage, rice straw mulching had the fewest number of leaves, roots, stems, the smallest width of leaves, and the least fresh weights of shoots and rhizomes. Combined mulching of black PE and rice hull showed the highest values in root-related characters; number of roots, fresh weights of rhizomes and roots. In its late stage, the treatment effects shown in the early stage disappeared owing to high temperature of mid-summer so that there was no significant difference between the mulching treatments. Yield per unit area and marketable yield were the highest in rice straw mulching, and combined mulching of black PE and rice hull.
Growth and Yield of Atractylodes japonica Koidz. Affected by Shading and Flower Bud Pinching
Park, Jeong-Min ; Kang, Jin-Ho ; Kim, Man-Bae ;
Korean Journal of Medicinal Crop Science, volume 12, issue 3, 2004, Pages 231~236
Crop productivity would be determined by relative capacity of source and sink. The study was carried out to measure the effects of shading and flower bud pinching on growth and yield of Atractylodes japonica Koidz. Shading treatment after transplanting was done at 100%, 65, 45 or 25% level of natural light intensity but the pinching treatment was to remove all the inflorescences formed after June 10, July 10, August 10 or none. Growth and yield at early and late stages were periodically measured. At the early stage, shading mainly influenced fresh weight rather than morphological characters. At the late stage, however, severer shading decreased numbers of stems, leaves, roots, fraction and total fresh weight. 65% shading from July 1 to September 30 after skipped the emerging and early growth stages showed nearly same growth and morphological characters but more rhizome yield per unit area than non-shading. In flower bud pinching treatment, earlier pinching increased number of roots, fraction fresh weights per plant and rhizome yield per unit area.
Antioxidant Activities of Leaf, Stem and Root of Panax ginseng C. A. Meyer
Lee, Seung-Eun ; Lee, Sung-Woo ; Bang, Jin-Ki ; Yu, Young-Ju ; Seong, Nak-Sul ;
Korean Journal of Medicinal Crop Science, volume 12, issue 3, 2004, Pages 237~242
To develop a new functional material, leaf, stem and root of Panax gjnseng were analyzed in their antioxidant activities. Root and leaf of ginseng collected from 3 regions, exhibited inhibition activity as
on peroxidation of low density lipoprotein and linoleic acid, respectively. Scavenging activities of stem, leaf and root of ginseng on superoxide anion radical were
, respectively. Total phenol contents of leaf, stem and root of ginseng were
respectively as tannic acid equivalent.
Comparison of Immune Activities of Essential Oils from Juniperus rigida S. et Z. and Boswellia carteii Birew by Supercritical Fluid Extraction System
Mun, Hyoung-Chul ; Park, Jin-Hong ; Kim, Dae-Ho ; Yoo, Jae-Eun ; Kim, Jung-Hwa ; Kim, Chang-Ho ; Kim, Jong-Dai ; Park, Young-Sik ; Lee, Hak-Ju ; Lee, Hyeon-Yong ;
Korean Journal of Medicinal Crop Science, volume 12, issue 3, 2004, Pages 243~248
Essential oils from Needl Juniperrus seed and trunk (Juniperrus rigida Sieb.) and Olibanum resin (Boswellia carteii Birew) were extracted by a supercritical fluid extraction system (SFE) and immune activity of each essential oils were observed. The immune activities of each essential oil were compared. Essential oil from Olibanum resin enhanced the growth of human immune T cell up to 1.33 times, compared to control group. Each essential oils showed the potent inhibitory effect on the human cancer cell lines, and increased the secretion of cytokines, IL-6 and
from human B cell as well as the growth of human immune cells.
Sap Collection and Major Components of Acer okamotoanum Nakai Native in Ullungdo
Moon, Hyun-Shik ; Kwon, Su-Duk ;
Korean Journal of Medicinal Crop Science, volume 12, issue 3, 2004, Pages 249~254
The collecting amount by DBH class and contents of mineral and free sugar in the sap of Acer okamotoanum Nakai being autogenous in Ullungdo were investigated. The total amount of sap was 376 l Sap of A. okamotoanum were increased with increasing DBH, 67 l (18%), 121 l (32%) and 188 l (50%) from small (below 18 cm), middle
and large (above 30cm) diameter tree, respectively. Amounts of sap was required by big temperature fluctuation in day and night. The contents of solid, ash and sugar were 3.04%, 0.06% and 3.06% in the sap of A. okamotoanum. Free sugar determined in the sap was sucrose with 30.6 g/l, but glucose, fructose and maltose were not detected. The sap of A. okamotoanum was composed of seven kinds of mineral. The prominent minerals in the sap were Ca and K, and the concentrations of Ca and K were 522 mg/l and 309 mg/l, respectively.
A Cytotoxic Compound from Moutan Cortex Radicis
Chu, Bo-Yeun ; Jin, Wen-Yi ; Choi, Su-La ; Myung, Pyung-Keun ; Seong, Yeon-Hee ; Song, Kyung-Sik ; Bae, Ki-Hwan ;
Korean Journal of Medicinal Crop Science, volume 12, issue 3, 2004, Pages 255~261
The MeOH extracts from 21 species were tested for their cytotoxicity against SK-MEL-28 melanoma cell line and HaCat normal cell line in 5 g/ml by sulforrhodamine-B (SRB) method. Among them, the MeOH extract from Moutan Cortex Radicis showed the moderate activity with the growth rate of 74.3% in SK-MEL-28 cells and the high activity with the growth rate of 207.8% in HaCat cells. Activity-guided fractionation was performed and five compounds, paeonol (1), benzoylpaeoniflorin (2), benzoic acid (3), 2,5-dihydroxy-4-methoxy- acetophenone (4), paeonfliorin (5) were isolated from hexane and EtOAc fraction. The structures were established by physicochemical and spectrometric methods
. All compounds were evaluated for their cytotoxicity, compound 4 showed the significant cytotoxic activity with
, but the other compounds no activity. These results suggest that compound 4 is a novel anticancer candidate against SK-MEL-28 melanoma cells.
Standardization of Geniposide Content of Gardeniae Fructus
Zheng, Ming-Shan ; Cai, Xing-Fu ; Choi, Ill-Sup ; Kim, Young-Ho ; Kang, Jong-Seong ; Bae, Ki-Hwan ;
Korean Journal of Medicinal Crop Science, volume 12, issue 3, 2004, Pages 262~265
Gardeniae Fructus (Gardenia jasmmoides Ellis) is widely used as a natural food colorant and as a traditional medicine for the treatment of hepatic and inflammatory diseases. The quality evaluation of Gardeniae Fructus was carried out by HPLC using geniposide as a marker. The geniposide was isolated from commercial Gardeniae Fructus on ODS column using a mixed solvent of acetonitrile-water (v/v 9:91) as mobile phase and a detection wavelength 240 mm. The content of geniposides in twenty different samples was in a range of 1.20% to 7.17% of total tissues and the average was 4.97%.