Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Medicinal Crop Science
Journal Basic Information
Journal DOI :
The Korean Society of Medicinal Crop Science
Editor in Chief :
Volume & Issues
Volume 12, Issue 6 - Dec 2004
Volume 12, Issue 5 - Nov 2004
Volume 12, Issue 4 - Sep 2004
Volume 12, Issue 3 - Jun 2004
Volume 12, Issue 2 - May 2004
Volume 12, Issue 1 - Mar 2004
Selecting the target year
Effect of Split Application of Nitrogen Fertilizer on Growth and Yield in Platycodon grandiflorum A. DC.
Seong, Jae-Duck ; Kim, Geum-Soog ; Kim, Hyun-Tae ; Park, Chung-Berm ; Kim, Seong-Man ;
Korean Journal of Medicinal Crop Science, volume 12, issue 6, 2004, Pages 437~441
These studies were conducted to investigate the proper method of nitrogen application for increasing the growth stability and quality in ballon flower (Platycodon grandiflorum A. DC.). In growth characteristics by split ratio of nitrogen application, stem diameter, number of branching roots and number of leaves were higher at the split ratio of 50:50 (nitrogen were splitted a half with basal application and the other half in autumn) than at 70:30. Root length, root diameter and number of branching root were higher at 50:50 than those of 70:30. The root yield was increased by 30% (230 kg/10a) at 50:50 compared with that of the 70:30. The crude saponin contents of root from 50:50 was 4.68%.
Production of Gomisin J from Cell Suspension Cultures of Schisandra chinensis Baillon
Hwang, Sung-Jin ; Pyo, Byoung-Sik ; Lee, Hak-Ju ; Hwang, Baik ;
Korean Journal of Medicinal Crop Science, volume 12, issue 6, 2004, Pages 442~447
Cell growth and gomisin J production by suspension cultures of Schisandra chinensis Baillon were investigated under various culture media, initial sucrose concentrations, shaking speeds, and inoculum sizes. Callus was induced from in vitro cultivated leaf segments on MS medium supplemented with
NAA. The maximum dry cell weight of 2.23 g was obtained at inoculum size of 0.5 g fresh cell weight and in MB5 medium supplemented with
NAA, 3% sucrose after 8 weeks. The production of gomisin J in suspension cell cultures was maximized in WPM medium containing 5% sucrose. The shaking speed for maintaining maximal cell dry weight was 100 rpm while the best shaking speed for gomisin J accumulation was 140 rpm.
Detection of Lignans from Transformed Root Cultures of Schisandra chinensis Baillon
Hwang, Sung-Jin ; Pyo, Byoung-Sik ; Hwang, Baik ;
Korean Journal of Medicinal Crop Science, volume 12, issue 6, 2004, Pages 448~453
Transformed roots of Schisandra chinensis were obtained following co-cultivation of in vitro cultivated plantlet segments with Agrobaterium rhizogens ATCC15834. This root was examined for its growth and gomisin J contents under various culture conditions. Among the six basal culture media tested, WPM (Lloyd & McCown, 1980) medium supplemented with 5% sucrose was the best roots growth 6.2 (g D.W/flask) and gomisin J accumulation 1.56
. Initial inoculum size correlated with the yield of biomass while gomisin J contents was not affect. Gomisin J production was influenced by the initial sucrose concentration and the highest production yield was achieved at the concentration of 7%. The optimal shaking speeds for roots growth and gomisin J production was 120 and 140 rpm, respectively.
Effect of Sulfur Fertilization Method on Quality of Safflower Seed
Kim, Min-Ja ; Kim, In-Jae ; Nam, Sang-Young ; Lee, Cheol-Hee ; Song, Beom-Heon ;
Korean Journal of Medicinal Crop Science, volume 12, issue 6, 2004, Pages 454~458
This study was carried out to clarify the effect of sulfur fertilization method on the qualities of safflower (Carthamus tinctorious L.) seed. Sulfur application increased the nitrogen and sulfur content of seed. In nitrogen content, there was no significant difference among sulfur fertilizers, whereas sulfur content was increased by raising ammonium sulfate (AS) amounts by soil application. In application method of AS, there was no significantly difference between foliar application and soil application with 8 kg S/10a. N/S ratio in seed was decreased by sulfur application, and also decreased with the increase of AS amounts by soil application. The contents of lipid and protein were increased by sulfur application. Lipid content was highest in foliar application, and protein content was lowest in AS 4 kg S/10a. By sulfur application, the content of total phenolics and electron donating ability (EDA) were increased by
, respectively compared with control. In content of total phenolics, there was no significant difference among sulfur fertilizers, but EDA was higher in sulfur powder 20 kg/10a and AS 8 kg S/10a than that in other fertilizers. Sulfur application showed positive effects on the qualities of safflower seed. In application effects, AS and foliar application were more efficient than sulfur powder and soil application, respectively.
Characteristics of Volatile Oil Components in Elsholtzia splendens Nakai Collected in Korea
Song, Song-Eui ; Chae, Young-Am ;
Korean Journal of Medicinal Crop Science, volume 12, issue 6, 2004, Pages 459~462
Essential oil components were analysed in forty seven individual plants of Elsholtzia splendens collected from eight different regions in Korea to identify their chemotypes. Major oil components of chemotype 1 was dihydrotagentone (75%) and naginataketone and elsholtziaketone were not detected at all. Chemotype 2 was naginataketone (NK) type which content was more than 60%. Chemotype 3 had more than 60% of elsholtziaketone (EK) as major volatile oil. EK type and NK type plants selected were maintained stably in their progenies after seed generation. Naginatketone and elsholtziaketone had functional properties such as antioxidation and antibacteria.
Chemical Composition and Biological Activities of Elsholtzia ciliata (Thunb.) Hylander
Jeong, Jae-Hoon ; Lim, Heung-Bin ;
Korean Journal of Medicinal Crop Science, volume 12, issue 6, 2004, Pages 463~472
This study was carried out to investigate the chemical composition of essential oils, absolutes and oleoresins isolated from Elsholtzia ciliata and the biological activities of them. Yields of essential oils, absolutes and oleoresins were 0.34%, 11.33% and 15.24%, respectively. The major component was naginate ketone in essential oils, methyl linolenate in absolutes and 9,12,15-octadecatrienoic acid in oleoresins. Eseential oils and oleoresins showed the inhibitory activities in enzyme-dependent, enzyme-independent and autooxidatve lipid peroxidation systems.
values in neutral red uptake assays 24 h of exposure times were
in essential oils, absolutes and oleoresins, respectively, and essential oils and oleoresins showed the cytotoxic effect at the only high dose. Absolutes and oleoresins did not show antibiotic and mutagenic activities. On the contrary, essential oils with over
showed antibiotic and mutagenic activities in Ames test. Essential oils and oleoresins have a prolongating effect the ciliostasis of rat trachea.
Development of Upland Cultivation for Production of Marketable Rhizomes in Wasabia japonica Matsum.
Moon, Jung-Seob ; Jang, Young-Gik ; Choi, Dong-Chil ; Choi, Joung-Sik ; Kim, Hyung-Moo ; Choi, Yeong-Geun ;
Korean Journal of Medicinal Crop Science, volume 12, issue 6, 2004, Pages 473~477
Wasabis (Wasabia japonica Matsum.) have been used as a spice with its petioles and rhizomes, but the production of its rhizomes in upland cultivation was very difficult for the severe damage of rotting disease during summer season. This experiment was carried out to increase the yield of marketable rhizome in wasabi when cultured in upland. Seedlings were raised in sub-alpine area for 7 and 12 months and then transplanted to experimental area on Sept. 20 and the yield was investigated on May 12 of the next year. The marketable rhizome(above 40g/plant) was produced in seedlings raised for 7 months. Production rate of marketable rhizome was 13.3% and total yield was 72.9 kg/10a. The content of allylisothiocyanate in rhizome of upland wasabi was 0.777 mg/g and its content was equal to wasabi cultured in water condition for 18 months. These results suggested that the upland cultivation of wasabi seedlings raised in sub-alpine area for 7 months was possible to product marketable rhizome.
Production of Gomisin J from Suspension Cultured Cells of Schisandra chinensis Baillon in Airlift-type Bioreactor
Hwang, Sung-Jin ; Pyo, Byoung-Sik ; Hwang, Baik ;
Korean Journal of Medicinal Crop Science, volume 12, issue 6, 2004, Pages 478~482
Suspension culture of Schisandra chinensis for production of gomisin J was perfomed in bioreactor. The inoculum size and initial sucrose concentration had significant effect on the cell growth and gomisin J accumulation. The maximum dry cell weight
and gomisin J content
were obtained at inoculum size of 100 g fresh cell weight (FCW) per liter and MB5 medium containing 6% sucrose after 8 weeks of culture. The effect of oxygen supply on the cell growth and gomisin J accumulation was also investigated in an airlift-type bioreactor. The optimal cell growth and gomisin J content was obtained under 0.5 vvm. The productivity of gomisin J was 0.7 fold in bioreactor culture lower than that obtained in a flask cultivation.
Seasonal Changes of Growth and Extract Content of Roots in Panax ginseng C. A. Meyer
Lee, Sung-Woo ; Kang, Seung-Won ; Seong, Nak-Sul ; Hyun, Geun-Su ; Hyun, Dong-Yun ; Kim, Young-Chang ; Cha, Seon-Woo ;
Korean Journal of Medicinal Crop Science, volume 12, issue 6, 2004, Pages 483~489
Monthly changes of underground growth and ethanol extract content was investigated from April to September in Korean ginseng of 4, 5 and 6 year-old. Taproot's length was repeatedly reduced and recovered without increasing by growth duration, while it's diameter was gradually increased until September of harvest time. Top weight reached to the peak on July, and decreased gradually until harvest time. Root weight of 4, 5 and 6-year-old ginseng was decreased on May, but increased gradually until harvest time except 6-year-old ginseng decreased after July. Relative growth rate (RGR) of root per plant was low on May and August, but high on June, July, and September in 4, 5 and 6 year-old ginseng. RGR of 4 and 5-year-old ginseng in each lateral and fine root showed distinct changes by month, while those showed little seasonal changes in 6 year-old ginseng. Ratio of root above 60g on september was 23%, 60% and 69%, respectively, among 4, 5 and 6 year-old ginseng. Moisture content of root was gradually decreased until september without significant difference from July to september in 4, 5 and 6 year-old ginseng, and its descending order on september was 4, 5 and 6 year-old ginseng. Hardness of taproot was decreased on May, but increased until september in 4 and 5 year-old ginseng, while that of 6 year-old ginseng was gradually decreased from August to september. Extract content of 4, 5 and 6 year-old ginseng in taproot and lateral root was distinctly decreased from March to June, but gradually increased until September. Although extract content of March and May in 4 year-old ginseng on showed higher than that of 5 and 6 year-old ginseng, it of September didn't show significant difference among 4, 5 and 6 year-old ginseng.
Karyotype Analysis of Five Species of Genus Pulsatilla
Lee, Woo-Kyu ; Choi, Hae-Woon ; Bang, Jae-Wook ;
Korean Journal of Medicinal Crop Science, volume 12, issue 6, 2004, Pages 490~493
Karyotypes were established in five Pulsatilla species from Korea : P. cernua, P. davurica, P. koreana, P. chinensis and P. tongkangensis. The somatic chromosome numbers of five species were all 2n=2x=16 with the basic number of x=8. The chromosome complement of P. cernua consisted of 5 pairs of metacentric, 1 pair of submetacentric and 2 pairs of subtelocentric. P. davurica, P. koreana and P. chinensis consisted of 5 pairs of metacentric and 3 pairs of subtelocentric. P. tongkangensis consisted of 5 pairs of metacentric, 2 pairs of submetacentric, and 1 pair of subtelocentric. Karyotype formulas of P. davurica, P. koreana, and P. chinensis were the same as K (2n) = 2x = 16 = 10m + 6st, while those of P. cernua was K (2n) = 2x = 16 = 10m + 2sm + 4st and P. tongkangensis was K (2n) = 2x = 16 = 10m + 4sm + 2st, respectively.
Long-Term Cold Storage and Plant Regeneration of Suspension Cultured Somatic Embryos of Eleutherococcus senticosus Maxim
Li, Cheng Hao ; Lim, Jung-Dae ; Heo, Kwon ; Kim, Myong-Jo ; Lee, Chan-Ok ; Lee, Jae-Geun ; Cui, Xue Shu ; Yu, Chang-Yeon ;
Korean Journal of Medicinal Crop Science, volume 12, issue 6, 2004, Pages 494~499
A method for long-term conservation of somatic embryos of Eleutherococcus senticosos was described. Suspension cultured globular somatic embryos were successfully conservated for 36 months at
. The embryos resumed growth within two weeks when returned to MS liquid medium containing
. 2,4-dichlorophenoxy acetic acid. The optimal condition for cell proliferation was achieved when somatic embryos cultured at
in 1/3 MS liquid medium, and about 1.2 g of embryogenic cell was induced from 150 globular embryos after 6 weeks of suspension culture. The embryogenic cells produced from these somatic embryos exhibited normal plant regeneration on auxin-free medium.
Regeneration Ability in Germplasms of Perilla frutescens
Lee, Chan-Ok ; Li, Cheng Hao ; Lim, Jung-Dae ; Yu, Chang-Yeon ;
Korean Journal of Medicinal Crop Science, volume 12, issue 6, 2004, Pages 500~507
The establishment of an efficient protocol of plant regeneration from leaf explant cultures of Perilla spp. is reported. Regenerated shoots were obtained from leaf explant cultures on solid MS medium containing different concentrations of cytokinins and auxin. The effect of cytokonin and auxin differed depending on each acession. The combination treatments of high level of cytokinin and low level of auxin was more effective for plant regeneration in Perilla frutescens. The best concentration of sucrose was 3% for regeneration. Of spermidine, spermin and putrescine. treatments, the most effective treatment for plant regeneration was
Effect of ABA and Salicylic Acid on Yields, Mineral Contents and Active Components of Chrysanthemum boreale M.
Lee, Kyung-Dong ; Tawaha, Abdel R.M. ; Yang, Min-Suk ;
Korean Journal of Medicinal Crop Science, volume 12, issue 6, 2004, Pages 508~514
Chrysanthemum boreale M. is an important medicinal plant that has been historically used in herbal medicine and in the health food throughout East Asia. This study was conducted to investigate the influence of abscisic acid (ABA) and salicylic acid (SA) on plant growth, mineral content and effective components, such as essential oil, amino acid and cumambrin A, by means in order to increase the productivity and the quality of flowerheads in the plant. Yields of flowerheads were increased by 12.7%, 21.7% and 15.5% by ABA, SA and both treatments, respectively, as compared with the control. Inorganic nutrient content was changed by PGRs; SA treatment was increased by nitrogen, phosphorus and magnesium content but decreased by potassium of C. boreale M. flowerheads. Total content of amino acid was increased by SA but decreased by ABA treatment. Essential oil content and yields were increased to 9.7% and 33.8% by SA treatment. Moreover, the content of terpene, monoterpenoids and sesquiterpenoids, were improved by ABA treatment, especially, germacrene-D content was increased by 39.1%, as compared to control. In addition, yields of cumambrin A, sesquiterpene compound exhibiting blood-pressure activity, increased in all PGRs treatments, but its concentration in the C. boreale M. flowerheads only increased by ABA and both treatment. The experiment suggests that PGRs using ABA and SA could increase the yields and quality of C. boreale M. flowerheads.
Physical Mapping of rDNAs Using McFISH in Anemarrhena asphodeloides Bunge
Kim, Soo-Young ; Choi, Hae-Woon ; Bang, Jae-Wook ;
Korean Journal of Medicinal Crop Science, volume 12, issue 6, 2004, Pages 515~518
Anemarrhena asphodeloides, a medicinal plant, has chromosome number of 2n=2x=22. To characterize the somatic metaphase chromosomes, physical mapping of 45S and 5S rDNAs using McFISH (multi-color fluorescence in situ hybridization) was applied. Two pairs of 45S rDNA loci were detected on the terminal regions of the short arm of chromosomes 1 and 3. A pair of 5S rDNA signal was observed on the short arm of chromosome 3. 5S rDNA site seemed to be the same locus as one of the 45S rDNA site. McFISH was very useful tool for the localization and identification of rDNAs on the metaphase chromosomes in A. asphodeloides.
New Variety 'Sanggang' of Coix lachryma-jobi L. with Early Maturity and Resistance to Leaf Blight
Yi, Eun-Seob ; Kim, Ki-Jung ; Kim, Hee-Dong ; Kim, Sung-Kee ; Chung, Kil-Woong ;
Korean Journal of Medicinal Crop Science, volume 12, issue 6, 2004, Pages 519~522
'Sanggang' was a new adlay cultivar which was developed from the three way crosses of
Suwon-6 at the Northern Agricultural Research Station, Gyunggido ARES. This cultivar had green sheaf, dark-green leaf, green culm, milky white silk and darkbrown seedcoat. Sanggang shortened 10 days of heading date and 12 days of ripening time compared with Yulmoo 1. In the yield's conponent of Sanggang, the number of grains was by 56 grains less but ripening rate and 1,000 grain weight were higher by 6.5% and 20.1 g compared with Yulmoo 1, respectively. This cultivar showed moderate resistance to leaf blight. The yield of Sanggang was about 313 kg/10a of unpolished grain at ordinary cultivation. This cutivar showed about 17% and 27% higher yield in Yonchon Gyeonggi province and Cheongju Chungbuk province compared with the check variety respectively. This cultivar was adaptable to mono cropping in central and northern region of Korea.