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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Journal of Medicinal Crop Science
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Journal DOI :
The Korean Society of Medicinal Crop Science
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Volume & Issues
Volume 13, Issue 6 - Dec 2005
Volume 13, Issue 5 - Oct 2005
Volume 13, Issue 4 - Aug 2005
Volume 13, Issue 3 - Jun 2005
Volume 13, Issue 2 - Apr 2005
Volume 13, Issue 1 - Feb 2005
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Comparison of Genetic Diversity and Population Structure of Kalopanax pictus (Araliaceae) and its Thornless Variant Using RAPD
Huh, Man-Kyu ; Jung, Sang-Duk ; Moon, Heung-Kyu ; Kim, Sea-Hyun ; Sung, Jung-Sook ;
Korean Journal of Medicinal Crop Science, volume 13, issue 2, 2005, Pages 69~74
Kalopanax pictus is a long-lived woody species mostly distributed in East Asia. K. pictus has been regarded as medically and ecologically important species in Korea. Thornless castor aralia variant, local name 'Cheongsong' is an endemic to Cheongsong province in Korea. Random amplified polymorphic DNA (RAPD) was used to investigate the genetic variation and structure of Korean populations of two species. A high level of genetic variation was found in six K. pictus populations. Twelve primers revealed 49 loci, of which 29 were polymorphic (59.2%). Nei's gene diversity for K.pictus and K. pictus variant were 0.119 and 0.098, respectively. Mean of genetic diversity in K. pictus was higher than average values for species with similar life history traits. The asexual and sexual reproduction, perennial habitat, and longevity are proposed as possible factors contributing to high genetic diversity. An indirect estimate of the number of migrants per generation (Nm=0.857) indicated that gene flow was not extensive among Korean populations of K.pictus. It is suggested that the isolation of geographical distance and reproductive isolation between K.pictus and K.pictus variant populations may have played roles in shaping the population structure of this species.
The Constituents Isolated from Peucedanum japonicum Thunb. and their Cyclooxygenase (COX) Inhibitory Activity
Zheng, Mingshan ; Jin, Wenyi ; Son, Kun-Ho ; Chang, Hyeun-Wook ; Kim, Hyun-Pyo ; Bae, Ki-Hwan ; Kang, Sam-Sik ;
Korean Journal of Medicinal Crop Science, volume 13, issue 2, 2005, Pages 75~79
Five coumarins, psoralen (1), scopoletin (2), isoimperatorin (4), (+)-marmesin (5) and xanthotoxin (6), three chromones, cimifugin (3), hamaudol (7) and sec-O-glucosylhamaudol (10), one sterol, daucosterol (8) and one aliphatic alcohol, galactitol (9) were isolated from the root of Peucedanum japonicum. Their chemical structures were identified by the physicochemical and spectroscopic data by comparing literature values. Among them, compounds 9 and 10 were isolated for the first time from this plant. The anti-inflammatory effects of isolated compounds were examined on cyclooxygenase (COX), compounds 1, 2 and 7 showed inhibitory activity on COX-1 with
values of 0.88, 0.27 and 0.30 mM, respectively. In the test for COX-2 activity, only compound 7 showed significant inhibitory activity with the
value of 0.57 mM. The other compounds exhibited weak inhibitory or no inhibitory activity.
Cytotoxic Anthraquinones and Stilbenes from Reynoutria sachalinensis (Fr. Schm.) Nakai
Jin, Wenyi ; Na, Min-Kyun ; Song, Gyu-Yong ; Lee, Young-Mi ; Bae, Ki-Hwan ;
Korean Journal of Medicinal Crop Science, volume 13, issue 2, 2005, Pages 80~84
Five known anthraquinones, physcion (1), I-O-methylemodin (2), emodin (3),
(6) and two known stilbenes, trans-resveratrol (4),
(7) were isolated from MeOH extract of Reynoutria sachalinensis (Polygonaceae). All structures were unambiguously established by 1D and 2D NMR and MS data and the compounds were evaluated for their cytotoxicity against L1210, HL-60, BI6F10 tumor cell lines in MTT assay. Among the compounds, trans-resveratrol (4) exhibited significant cytotoxic activity with
values of 9.2, 6.7 and
, against the test cell lines respectively, but compounds 1-3 exhibited the moderate cytotoxic activity.
Constituents and their DPPH Scavenging Activities from the Leaves of Alnus hirsuta (Spach) Rupr.
Dai, Yinghui ; Thuong, Phuong Thien ; Hung, Tran Manh ; Jin, Wenyi ; Cui, Zheng ; Bae, Ki-Hwan ;
Korean Journal of Medicinal Crop Science, volume 13, issue 2, 2005, Pages 85~90
Phytochemical study on the EtOAc fraction from a MeOH extract of the leaves of Alnus hirsuta Rupr. led to the isolation of nine compounds betulin (1), betulinic acid (2), hirsutanonol (3), hirsutenone (4), quercetin (5), avicularin (6), gallic acid (7), hyperin (8), and daucosterol (9). Among them, six compounds 1, 2, 57, and 9 are report from this plant for the first time. All isolated compounds were evaluated for their antioxidant activity using DPPH radical scavenging capacity and inhibition effect on mitochondrial lipid peroxidation. Six phenolic compounds 3-8 were found to have potent antioxidant activity. Of which, compounds 3, 4 and 5 showed significant free radical scavenging activity with the
, respectively. In addition, the compounds 3-8 exhibited inhibition effect on the mitochondrial lipid peroxidation with the
Effects of Precursor and Thidiazuron on Triterpene Saponin Biosynthesis in Centella asiatica (L.) Urban
Kim, Ok-Tae ; Kim, Min-Young ; Ahn, Jun-Cheul ; Li, Mei-Yang ; Hwang, Baik ;
Korean Journal of Medicinal Crop Science, volume 13, issue 2, 2005, Pages 91~94
Plants have been known to accumulate a very diverse range of triterpene saponins. We have investigated the regulation of saponin biosynthesis in higher plants using Centella asiatica (L.) Urban as a model plant. Effects of a feeding precursor on asiaticoside production from leaves and on the level of two-type OSCs mRNA were investigated. As a feeding precursor, squalene negatively affected the levels of CYS and bAS mRNA, but it also decreased the production of asiaticoside from whole plants. Plant hormones regulate secondary metabolism, and in plant tissue cultures they could affect both culture growth and secondary metabolite production. Although enhancement of asiaticoside production from whole plant cultures by addition of TDZ (thidiazuron) has been reported, the positive effect of TDZ on the levels of OSCs transcripts was not observed.
Protection of Amyloid
Protein (25-35)-induced Neuronal Cell Damage by Methanol Extract of New Stem of Phyllostachys nigra Munro var. henonis Stapf in Cultured Rat Cortical Neuron
Ban, Ju-Yeon ; Cho, Soon-Ock ; Kwon, Soon-Ho ; Kim, Jin-Bae ; Song, Nak-Sul ; Bae, Ki-Whan ; Song, Kyung-Sik ; Seng, Yeon-Hee ;
Korean Journal of Medicinal Crop Science, volume 13, issue 2, 2005, Pages 95~102
Caulis Bambusae in Taenia is widely used in Korea and China due to its various pharmacological activity. The present study aims to investigate the effect of the methanol extract of Caulis Bambusae in Taenia (CB) from Phyllostachys nigra Munro var. henonis Stapf (Gramineae) on amyloid
, a synthetic 25-35 amyloid peptide, -induced neurotoxicity using cultured rat cortical neurons. CB, over a concentration range of
, inhibited the
-induced neuronal cell death, as assessed by a 3-[4,5-dimethyIthiazole-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and the number of apoptotic nuclei, evidenced by Hoechst 33342 staining. CB
inhibited glutamate release into medium induced by
, (25-35) which was measured by HPLC. Pretreatment of CB
(25-35)-induced elevation of cytosolic calcium concentration
, which was measured by a fluorescent dye, fluo-4 AM, and generation of reactive oxygen species. These results suggest that CB prevents
(25-35)-induced neuronal ell damage in vitro.
Screening of Anti-stress Activities in Extracts from Korean Medicinal Herbs
Kim, Dae-Ho ; Kim, Jung-Hwa ; Kim, Cheol-Hee ; You, Jin-Hyun ; Hwang, Baik ; Lee, Hak-Ju ; Kim, Jong-Dai ; Lee, Hyeon-Yong ;
Korean Journal of Medicinal Crop Science, volume 13, issue 2, 2005, Pages 103~109
When we carried out an anti-stress study using the extracts of A. senticosus, R. coreanus, F .japonica root, and A. fruticosa fruit grown in Korea, the results showed that blood cell counts returned to normal levels fastest with R. coreanus after stress application. The total WBC count was decreased in mice due to stress, whereas the number of lymphocytes was increased. The serum glucose level was higher in the control group compared with the comparative stress group. The weight of each organ to returning to normal level was significantly faster in those mice treated with the extracts compared with those in control groups. Especially, it even return to that of normal level with R. coreanus extract, suggesting that the administration of the plant extracts in this study would affect hormonal activities in the body to increase immune activities. F. japonica root that showed the highest anti-stress activities maybe effective for lipid and carbohydrate metabolism. These results would provide basic data to examine anti-stress effects of natural plants. Further in-depth studies could contribute in the development of functional plant materials with anti-stress activities.
Negative Effects of Water Extracts from Pinus densiflora Sawdust on Mycelium Growth of the Shiitake Mushroom Lentinula edodes
Choi, Myung-Suk ; Yang, Jae-Kyung ;
Korean Journal of Medicinal Crop Science, volume 13, issue 2, 2005, Pages 110~114
Extracts from sawdust of Pinus densiflora were showed antifungal activity against Lentinula edodes. It was extracted by hot water and then successively extracted by n-hexane, ethyl acetate, and methanol. The yields of the n-hexane-soluble, ethyl acetatesoluble, methanol-soluble and methanol-insoluble fractions of water extracts were 8.2%, 10.6%, 32.0%, and 49.2%, respectively. The ethyl acetate-soluble fraction showed the greatest antifungal activity against L. edodes: 41.5% inhibition at 1,000 ppm. However, there were not significant differences of antifungal activities between n-hexane-soluble fraction and methanol-soluble fraction at a concentration of 1,000 ppm. The hot water extracts showed 23.5% of antifungal activity against L. edodes at a concentration of 1000 ppm. The four antifungal compounds were separated from ethyl acetate fraction by thin layer chromatography.
Genetic Diversity and DNA Polymorphism in Platycodon grandiflorum DC. Collected from East-Asian Area
Park, Chun-Geun ; Yan, Zhi-Yi ; Lee, Sang-Chul ; Shon, Tae-Kwon ; Park, Hee-Woon ; Jin, Dong-Chun ;
Korean Journal of Medicinal Crop Science, volume 13, issue 2, 2005, Pages 115~120
Broadening the genetic base of Platycodon grandiflorum DC. cultivar to sustain improvement requires assessment of genetic diversity available in P. grandiflorum DC.. The objective of this study was to analyze the genetic variation, genetic relationship among 48 samples collected from East-Asian Area by means of RAPD-PCR (random amplified polymorphic DNA-polymerase chain reaction) markers. From the 18 primers tested, produced total 211 bands with an average of 11.7 bands per primer and obtained 103 polymorphic band with an average of 5.7 bands per primer,s revealed relatively high percentage of polymorphic bands (48.8%). The genetic similarities calculated from RAPD data varied from 0.688 to 0.994 and were clustered to six major groups on a criterion of 0.78 similarity coefficient. The present study has revealed the significant genetic similarity among the samples tested. The analysis of genetic relationships in P. grandiflorum using RAPD-PCR banding data can be useful for the breed improvement.
Selection of PCR Markers and Its Application for Distinguishing Dried Root of Three Species of Angelica
Jin, Dong-Chun ; Sung, Jung-Sook ; Bang, Kyong-Hwan ; In, Dong-Su ; Kim, Dong-Hwi ; Park, Hee-Woon ; Seong, Nak-Sul ;
Korean Journal of Medicinal Crop Science, volume 13, issue 2, 2005, Pages 121~125
An analysis of RAPD-PCR (random amplified polymorphic DNA-polymerase chain reaction) was performed with three Angelica species (A. gigas Nakai, A. sinensis (Olive.) Diels and A. acutiloba Kitag) in an effort to distinguish between members of these three species. Two arbitrary primers (OPC02, OPD11) out of80 primers tested, produced 17 species-specific fragments among the three species. Eight fragments were specific for A. sinensis, four fragments specific for A. gigas, five specific for A. acutiloba. When primers OPC02 and OPD11 were used in the polymerase chain reaction, RAPD-PCR fragments that were specific for each of the three species were generated simultaneously. Primer OPC02 produced eight species-specific fragments: four were specific for A. sinensis, one for A. gigas, and three for A. acutiloba. Primer OPD11 produced nine speciesspecific fragments: four for A. sinensis, three for A. gigas, and two for A. acutiloba. The RAPD-PCR markers that were generated with these two primers should rapidly identify members of the three Angelica species. The consistency of the identifications made with these species-specific RAPD-PCR markers was demonstrated by the observation that each respective marker was generated from three accessions of each species, all with different origins. We also performed the RAPD-PCR analysis with the dried Angelica root samples that randomly collected from marketed and from the OPC02 primer, obtained a A. gigasspecific band and the band were cloned and sequenced.
Metabolism Characteristics of Acifluorfen Tolerant in Somaclones of Eastern Black Nightshade (Solanum ptycanthum Dun.)
Yu, Chang-Yeon ; Li, Cheng Hao ; Lee, Dong-Wook ; Lim, Jung-Dae ; Kim, Jae-Kwang ;
Korean Journal of Medicinal Crop Science, volume 13, issue 2, 2005, Pages 126~130
Acifluorfen tolerance charateristics determined the involvement of absorption, translocation, and metabolism in acifluorfen tolerance. Less than 6% of the applied
was absorbed. There were no differences in acifluorfen absorption between susceptible and tolerant somaclones. More
was translocated in the susceptible than the tolerant somaclones. The susceptible somaclone did not metabolize acifluorfen while some somaclones (i.e.,EBN-3A) metabolized
. Nomenclature: Acifluorfen, 5-[2-chloro-4-(trifluoromethyl)phenoxy]-2-nitrobenzoic acid; eastern black night shade, Solanum ptycanthum Dun.,