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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Journal of Medicinal Crop Science
Journal Basic Information
Journal DOI :
The Korean Society of Medicinal Crop Science
Editor in Chief :
Volume & Issues
Volume 13, Issue 6 - Dec 2005
Volume 13, Issue 5 - Oct 2005
Volume 13, Issue 4 - Aug 2005
Volume 13, Issue 3 - Jun 2005
Volume 13, Issue 2 - Apr 2005
Volume 13, Issue 1 - Feb 2005
Selecting the target year
Investigation of Medicinal Substances from in vitro Cultured Cells and Leaves of Artemisia princeps var. Orientalis
Shin, Dong-Ho ; In, Jun-Gyo ; Yu, Sang-Ryul ; Choi, Kwan-Sam ;
Korean Journal of Medicinal Crop Science, volume 13, issue 3, 2005, Pages 69~76
The young leaves of A. princeps have been a well known for a crude medicine and used in treatment of colic pain, vomiting and menstrual irregularity. Based on TLC and HPLC and used an artemisinin, an anti-malarial compounds which is believed to be detected only in A. annuaup so far can be biosynthesized in A. princeps. To investigate the production of secondary metabolites like artemisinin in cultured cells, the cell culture of A. princeps was established. Callus and suspension cultured cells of A. princeps were induced and grown highest in MS media containing
BAP and 2% sucrose. Different metabolites from in vitro cultured cells (callus and suspension cultured cell) and intact plants were analyzed by TLC analysis. As a result, we can confirm that in vitro culture has a potential for mass production of secondary metabolites from A. princeps.
Several Factors Affecting to Rooting of Stem Cuttings in Rubus buergeri Miquel
Kang, Young-Kil ; Ko, Mi-Ra ; Kang, Si-Young ; Riu, Key-Zung ;
Korean Journal of Medicinal Crop Science, volume 13, issue 3, 2005, Pages 77~80
This study was conducted to determine effects of cutting date, cutting position, and node number on rooting of Rubus buergeri Miquel. Apical, medial, and basal parts of the vines emerged in 2002 were cut on June 26, August 2, and September 4, in 2002. Vine pieces with one to four nodes taken from the medial parts of the vines were also cut on July 10. The greatest rooting (69.6%) occurred in cuttings taken on August 2. Cutting taken on June 26 had the longest roots (23.1 cm). Rooting and new shoot growth markedly decreased at September 4 cutting. The greatest rooting (55.4%) occurred in cuttings taken from medial part of the vines and followed by apical parts (45.6%), and basal parts (25.4%). The other traits were not significantly affected by cutting position. Rooting percentage and percent new shoot growth were not significantly affected by node number, but root number and root dry weight per cutting, and new shoot growth increased with node number.
Enhancement of Immune Activities of Ephedrae Herba and Rubi Fructus at Low Temperature Extraction
Kim, Dae-Ho ; Park, Jin-Hong ; Kim, Jung-Hwa ; Kim, Cheol-Hee ; You, Jin-Hyun ; Kwon, Min-Chul ; Lee, Hyeon-Yong ;
Korean Journal of Medicinal Crop Science, volume 13, issue 3, 2005, Pages 81~86
The immune activities of the extracts from Ephedrae Herba and Rubi Frutus extraction with ultrasonification at
were compared with the extracts though water extraction at
. The growth of human T cells was increased up to
of the ultrasonification extracts of R. Fructus at
, compared to adding the extracts at
. The secretion of
for human T cells were also increased up to
pg/cell by adding extracts at
, compared to extracts at
. The extracts of R. Fructus at
increased NK cell activities up to 50% and the secretion of
from macrophage to
for ultrasonification extracts at
Screening of Anticancer and Immune Activities by the Extracts of Fatsia japonica Decne. et Planch.
Kim, Dae-Ho ; Kim, Jung-Hwa ; You, Jin-Hyun ; Kim, Cheol-Hee ; Kwon, Min-Chul ; Lee, Hak-Ju ; Hwang, Baik ; Lee, Hyeon-Yong ;
Korean Journal of Medicinal Crop Science, volume 13, issue 3, 2005, Pages 87~92
The study was performed using of ethanol and water extracts of Fatsia japonica Decne. et Planch. in anticancer and immune activities. All extracts of 1.0
concentration were increased in over 60% of the anticancer activites in A549 and MCF7 cells. Root barks inhibited 55%, 74% in A549 and MCF7 cell by adding ethanol extracts of
concentration. The cytotoxicity of human lung nomal cell (HEL299) counted up to about 22% for ethanol extracts of root barks in 1
concentration. The activity of human immune T and B cells were increased up to
by adding ethanol extract of the root barks. Increasing trend of secretion of cytokine (IL-6,
) from human B and T cell for 5 days cultivation has been abserved. From the results, the anticancer and immune-stimulatory activities of the roots extract were higher than the extracts of other parts.
Antifungal and Antioxidant Activities of Extracts from Leaves and Flowers of Camellia japonica L.
Lee, Sook-Young ; Hwang, Eun-Ju ; Kim, Gi-Hae ; Choi, Young-Bok ; Lim, Chae-Young ; Kim, Sun-Min ;
Korean Journal of Medicinal Crop Science, volume 13, issue 3, 2005, Pages 93~100
This research was performed to investigate the possibilities of industrial usage of camellia (Camellia japonica L.) by examining the antioxidant and antimicrobial effects of methanol extract with different sections. Content of total phenolics, DPPH radical scavenging activities and antibacterial activity of young leaf, mature leaf, flower bud, flower, bark, and seed of camellia were compared in vitro experimental models. Total phenolics was contained the higher in young leaf (74.62 mg), flower bud (65.02 mg) and flower (62.42 mg) but less than 20.95 mg per 100 g of dry weight in other parts of Camellia japonica L. And effects of antioxidant measured by DPPH radical scavenger activity (
, reduce concentration 50%), was shown higher
in methanol extract of young leaf, flower bud and flower than
of BHT as a chemical oxidant. Also, the antimicrobial activity of Camellia japonica L. extracts determined using a paper disc method against food-borne pathogen and food spoilage bacteria, the young leaves extracts showed the most active antimicrobial activity against 7 kinds of harmful microorganisms. Flower bud extracts showed the highest antibacterial activity against P. aeruginosa and Enterobacter spp. C1036. In addition, the minimum inhibitory concentration (MIC) of young leaf extract against B. subtillis,S. fradiae,S. aureus,E. coli,P. aeruginosa, Enterobacter spp. C1036, and S. typhimurium were revealed 1 to 15
. As a result, antimicrobial activity of camellia extracts was shown higher gram positive bacteria than gram negative bacteria.
Hypericin Contents of Hypericum erectum Thunberg
Kim, Sun-Hee ; Jung, Young-Jae ; Ahn, Jun-Cheul ; Hwang, Baik ;
Korean Journal of Medicinal Crop Science, volume 13, issue 3, 2005, Pages 101~104
To explore the Korea native plants to substitute for St. John's wort, which produce hypericin that use commercially for the treatment of mild to moderate depression, hypericin contents of Hypericum erectum and H. ascyron collected in two mountain of Korea were examined. From TLC and TLC-densitometer analysis of hypericin contents and biosynthetic pattern, hypericin was detected in flower and leaf of H. erectum, but not in all organs of H. ascyron. The hypericin content of H. erectum grown 200 m high hill in Mt. Byoung-pung was higher than that of Mt. Ji-ri 500 m and 1300 m high hill. When the seasonal variation of hypericin contents in H. erectum leaf collected from two regions was investigated, leaf collected from both regions on July was higher than other seasons.
Adventitious Bud Induction and Plant Regeneration from Cotyledon Explants of Camellia japonica L.
Kim, Kwang-Soo ; Hwang, Sung-Jin ; Pyo, Byoung-Sik ; Kim, Sun-Min ;
Korean Journal of Medicinal Crop Science, volume 13, issue 3, 2005, Pages 105~108
Culture conditions for plant regeneration of Camellia japonica were achieved by organogenesis in explants of cotyledon. Seed cotyledons were cultured on MS medium containing various auxin, 2,4-D or NAA and cytokinins BA. The adventitious shoot buds were efficiently formed without embryogenesis on the basal region of cotyledon cultured on MS medium supplemented with
BA. Seed cotyledons could be used as a source of explants in experiments of genetic transformation of the genotypes evaluated for improving the efficiency of production of transgenic Camellia plants.
Genetic Relationship among Ostericum koreanum Kitakawa Collections by RAPD Analysis
Kim, Soo-Yong ; Sim, Yong-Gu ; Kwon, Soon-Tae ; Oh, Sei-Myung ;
Korean Journal of Medicinal Crop Science, volume 13, issue 3, 2005, Pages 109~113
To analyze the genetic relationship 8 accessions of Ostericum koreanum Kitakawa, random amplified polymorphic DNA(RAPD) analysis was performed using 60 Operon primers. The 25 primers out of 60 random primers were amplified DNA by PCR using genomic DNA of O. koreanum. Eighty-five (49.1%) among 173 bands derived from 25 primers showed poly morphism, On the basis of similarity coefficient analysis by UPGMA, 8 accessions of O. koreanum Kitakawa could be classified into three groups at the similarity coefficient value of 0.71. Group I contained three accessions (Nam Gangwhal), Group II contained one accession (Nam Gangwhal) and Group III contained four accessions (Buk Gangwhal), The range of total genetic similarity coefficient value of 8 accessions of O. koreanum Kitakawa was
. Buk Gangwhal was flowered 18 to 26 days earlier than Nam Gangwhal, and Nam Gangwhal leaf stalk was thin and long as bolting rate high compared with Buk Gangwhal.
Growth and Flowering of Orostachys japonicus A. Berger by Controlling Daylengths
Kang, Jin-Ho ; Jeon, Seung-Ho ; Yoon, Soo-Young ; Hong, Dong-Oh ; Shin, Sung-Chul ;
Korean Journal of Medicinal Crop Science, volume 13, issue 3, 2005, Pages 114~117
Orostachys japonicus, called Wasong as herbal medicine and a short day plant, should be artificially kept in long daylength to control anthesis of its florets. The study was done to clarify the effect of daylength (10, 13, 16 hours a day) on growth, morphological characters, and flowering. The treatments were done on August 25 and afterward samples were taken every 2 weeks. Growth, morphological and flowering related characters were measured from each sample. With longer daylength plant height and inflorescence length were increased but number of leaves including bracts and stem diameter were decreased. Leaves and bracts, stem, root, shoot and total dry weights were increased with longer daylength from late September while floret dry weight showed reverse result. Florets formed were the greatest in daylength of 10 hours during September but the least during the other period. Anthesis of the florets was observed only in daylength of 10 hours during October and all the plants from the treatment were flowered.
Cytogenetic Analysis of Seven Angelica Species
Choi, Hae-Woon ; Koo, Dal-Hoe ; Lee, Woo-Kyu ; Kim, Soo-Young ; Sung, Jung-Sook ; Seong, Nak-Sul ; Suh, Young-Bae ; Bang, Jae-Wook ;
Korean Journal of Medicinal Crop Science, volume 13, issue 3, 2005, Pages 118~121
Karyotypes were established in seven Angelica species cultivated in Korea. The somatic chromosome numbers were 2n = 2x = 22 with the basic number of x = 11 in all Angelica plants examined. Their metaphase chromosomes ranged from 3.56
. to 8.91 x. in length. Distinctive Karyotypes were found in two species, A. tenuissima with all metacentries, K(2n) = 2x = 22m, and A. genuflexa with all subtelocentrics, K(2n) = 2x = 22st. Karyotype formulas of A. gigas, A. acutiloha, A. sinensis, A. decursiva and A. dahurica were K(2n) = 2x = 20m + 2sm, K(2n) = 2x = 12m + 10sm, K(2n) = 2x = 16m + 6sm, K(2n) = 2x = 18m + 4sm and K(2n) = 2x = 10m + 10sm + 2st, respectively. Cytological data showed that chromosomal polymorphisms within species were observed in Angelica plants compare to other regions.
New Variety 'Johyun' of Coix lacryma-jobi var. mayuen Stapf with Early Maturity and Short Plant Height
Jang, Jung-Hee ; Yi, Eun-Seob ; Choi, Byeong-Yeol ; Kim, In-Jae ; Park, Jung-Soo ; Kim, Sung-Kee ; Kim, Hee-Dong ;
Korean Journal of Medicinal Crop Science, volume 13, issue 3, 2005, Pages 122~125
‘Johyun’ was a new adlay variety which was developed from three way crosses of (Suwon-6xOkayama)x UCN300-25 at the Northern Agricultural Research Station, Gyeonggi Province ARES. This variety had purple sheath and green leaf blade in primitive growth stage and green leaf and culm in heading stage. Colors of pistil and seed coat were milky-white and dark-brown, respectively. Plant height of Johyun was 179 cm and shortened 22 cm less than Yulmoo-1 which was tested by check variety. Johyun shortened 8 days of heading date and 15 days of ripening date compared with Yulmoo-1. Yield of Johyun was 320 kg/10a of hulled grains in three regional yield trials and 18% higher than Yulmoo-1. This variety was adaptable to single cropping in central and northern region of Korea.