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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Journal of Medicinal Crop Science
Journal Basic Information
Journal DOI :
The Korean Society of Medicinal Crop Science
Editor in Chief :
Volume & Issues
Volume 13, Issue 6 - Dec 2005
Volume 13, Issue 5 - Oct 2005
Volume 13, Issue 4 - Aug 2005
Volume 13, Issue 3 - Jun 2005
Volume 13, Issue 2 - Apr 2005
Volume 13, Issue 1 - Feb 2005
Selecting the target year
Immunoregulatory Effects of Phellinus linteus (Berk. et Curt) Teng Extract on the Cytokine Production, T Cell Population and Immunoglobulin E Level in Murine Mesenteric Lymph Node Lymphocytes
Lim, Beong-Ou ; Hong, Doo-Pyo ; Yun, June-Yong ; Jeoung, Young-Jun ; Lee, Ju-Yeon ; Chung, Hae-Gon ; Choi, Dong-Kug ; Choi, Wahn-Soo ; Cho, Bong-Gum ; Park, Tae-Kyu ; Park, Dong-Ki ;
Korean Journal of Medicinal Crop Science, volume 13, issue 5, 2005, Pages 213~218
Phellinus linteus (PL), one of the immune-regulatory substances, is recognized to play the role in the metabolic process on inflammation and immunity. It has been traditionally used in the oriental medicine to treat inflammatory related disease. The purpose of this study was to evaluate the effects of water extracts of PL on the mesenteric lymph node lymphocytes immune function in the ICR male mice. Control mice received vehicle only. The PL treated mice were administered the respective extract by oral gavages for 4 weeks. IgE concentrations in serum and MLN lymphocytes were significantly lower in PL treated mice than in control mice. PL increased the proportion of
T cells in MLN lymphocytes. PL significantly decreased Th2 cytokine concentrations and mRNA expression levels in cytokine secretions. Therefore, water extracts of PL modulate inflammatory parameters through regulation of immunoglobulin production resulting from decreased Th2 cytokine secretion and mRNA expression levels and reduce pro-inflammatory cytokine secretion and mRNA expression in MLN lymphocytes.
Protective Effect of Sanguisorba officinalis L. Root on Amyloid
Protein (25-35)-induced Neuronal Cell Damage in Cultured Rat Cortical Neuron
Ban, Ju-Yeon ; Cho, Soon-Ock ; Jeon, So-Young ; Song, Kyung-Sik ; Bae, Ki-Hwan ; Seong, Yeon-Hee ;
Korean Journal of Medicinal Crop Science, volume 13, issue 5, 2005, Pages 219~226
Sanguisorbae radix (SR) from Sanguisorba officinalis L. (Losaceae) is widely used in Korea and China due to its various pharmacological activity. The present study aims to investigate the effect of the methanol extract of SR on amyloid
, a synthetic 25-35 amyloid peptide, -induced neurotoxicity using cultured rat cortical neurons. SR, over a concentration range of
, inhibited the
neuronal cell death, as assessed by a 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and the number of apoptotic nuclei, evidenced by Hoechst 33342 staining. Pretreatment of SR
(25-35)-induced} elevation of cytosolic calcium concentration
, which was measured by a fluorescent dye, fluo-4 AM. SR
inhibited glutamate release into medium induced by
, which was measured by HPLC, and generation of reactive oxygen species. These results suggest that SR prevents
(25-35)-induced neuronal cell damage in vitro.
Antioxidant Effect of Flavonoids Isolated from the Root of Clematis trichotoma Nakai
Hung, Tran Manh ; Thuong, Phuong Thien ; Bae, Ki-Hwan ;
Korean Journal of Medicinal Crop Science, volume 13, issue 5, 2005, Pages 227~232
The antioxidant effect of methanol extract (ME) and water extract (WE) from Clematis trichotoma was evaluated as primary study to scavenge stable 1,1-diphenyl-2-picrylhydrazyl radicals (DPPH), inhibited iron-induce lipid peroxidation in linoleic acid emulsion, peroxidation of liposome induced by
acid, and on
induced the mitochondrial lipid peroxidation. In secondary study, five flavonoids as luteolin (1), quercetin (2), apigenin (3), hirsutrin (4), kaempferol-3-O-glucoside were isolated (5). Among them, compounds 1 and 2 showed good activities in all the model systems. Compound 3 exhibited moderate antioxidant activities in both radical scavenging and these lipid peroxidation systems tested. Compound 4 showed significant inhibitions in liposome peroxidation and compound 5 displayed weak inhibition in all four tested systems. All the results presented herein indicate that products of C. trichotoma maybe useful in inhibiting membrane lipid peroxidation and preventing free radical-linked diseases.
Up-regulation of Asiaticoside Biosynthesis by Methyl Jasmonate and Thidiazuron in Centella asiatica L. Urban
Kim, Ok-Tae ; Kim, Min-Young ; Ahn, Jun-Cheul ; Hwang, Baik ;
Korean Journal of Medicinal Crop Science, volume 13, issue 5, 2005, Pages 233~236
Centella asiatica accumulates large amounts of triterpene saponin, such as centellasaponin, asiaticoside, madecassoside. We examined the effect of two candidates, MJ (Methyl jasmonate) and TDZ (thidiazuron), on asiaticoside production and the accumulation of bAS mRNA associated with asiaticoside biosynthesis in leaves of cultured whole plants. The growth of whole plants treated with 0.1 mM MJ was found to decrease significantly, however, the growth of whole plants treated with 0.1 mM MJ plus 0.025 mg/l TDZ was better than that treated with MJ alone. When MJ alone was added to culture medium, asiaticoside contents in leaves were higher than that of control after 7 days of treatments. The maximum level of bAS
mRNA in leaves of whole plant treated TDZ and MJ was transiently observed after exposure to 5 days. These results showed the up-regulation of bAS gene by adding TDZ and MJ at the molecular level, however, synergic effects of TDZ and MJ on asiaticoside biosynthesis were not testified.
Production of Tropane Alkaloids by Optimal Culture Conditions in Adventitious Root of Hyoscyamus niger L.
Park, Dong-Jin ; Min, Ji-Yun ; Kim, Yong-Duck ; Kang, Seung-Mi ; Jung, Ha-Na ; Kang, Hoon-Serg ; Choi, Myung-Suk ;
Korean Journal of Medicinal Crop Science, volume 13, issue 5, 2005, Pages 237~241
Scopolamine and hyoscyamine are important anticholinergic compounds obtained from Hyoscyamus niger. Adventitious roots induced from rhizome of H. niger and roots were cultured in SH medium supplemented with 3% (w/v) sucrose and 0.5 mg/L IBA. Roots were grown rapidly after 10 days of cultures. Scopolamine production was increased 7 times and hyoscyamine production was increased 12 times after 10 day of cultures. SH medium was best in root growth. But, highest scopolamine productivity was observed in WPM medium, followed White medium and best hyoscyamine productivity was resulted in MS medium. Sucrose was increased scopolamine and hyoscyamine production were increased the medium supplemented by sucrose comparing to than those by other carbon sources.
Physiological Changes with Age by the Chronic Administration of Korean Red Ginseng in Spraque-Dawley Rats
Lim, Heung-Bin ; Sohn, Hyung-Ok ; Lee, Dong-Wook ;
Korean Journal of Medicinal Crop Science, volume 13, issue 5, 2005, Pages 242~248
The present study was designed to elucidate the physiological changes with age by chronic administration of red ginseng. All rats were reared in the conventional system. Ginseng treated rats were continously supplied with ginseng water extracts together water from 6 weeks of age to the age 24 months. Rats did not show any discernible signs or the rejection symptoms by red ginseng water extracts. A long-term administration of red ginseng extracts did not cause any physiological changes in the gain of body and organs weight, food intake and general properties of urine. However, red ginseng caused to decrease the level of serum cholesterol, glucose and TBARS, and it attenuated effectively the age-dependent decline of LDH activity. Other biochemical parameters measured from blood and general properties of urine were not significantly changed. These results suggest that long-term administration of red ginseng to rat does not cause any clear physiological changes in appearance and urine, and it retards age-related deteriorations in some biochemical parameters such as LDL-cholesterol, glucose and LDH in serum.
Genetic Relationships of Panax Species by RAPD and ISSR Analyses
In, Dong-Su ; Kim, Young-Chang ; Bang, Kyong-Hwan ; Chung, Jong-Wook ; Kim, Ok-Tae ; Hyun, Dong-Yoon ; Cha, Seon-Woo ; Kim, Tae-Soo ; Seong, Nak-Sul ;
Korean Journal of Medicinal Crop Science, volume 13, issue 5, 2005, Pages 249~253
This study was carried out to develop convenient and reproducible methods for identifying the genetic relationship among germplasms of Panax species based on molecular genetics. Using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) analyses, genetic polymorphism of the Panax species was investigated with following cultivars and accessions, such as Chunpoong, Yunpoong, Kopoong, Sunpoong, and Kumpoong in domestic cultivars, Hwangsuk, Jakyung and Suckju in domestic accessions, and Panax quinquefolius L. and Panax japonicus C.A. Meyer in foreign introduced accessions, respectively. Specific DNA fragments ranging from 200 to 3,000 base pairs in size could be obtained with various ISSR and RAPD primers under the optimized PCR conditions. The dissimilarity coefficients among the genetic polymorphisms of ginseng cultivars and accessions were calculated from 0.26 to 0.90 in RAPD and from 0.12 to 0.89 in ISSR analysis, respectively. Eleven plant samples were grouped siblings together with cultivars and parents based on cluster analysis of genetic distance depending on genetic property such as origin of the species. In results, both RAPD and ISSR analyses were useful for identifying the genetic relationship among cultivars and accessions of Panax species at DNA level.
Effects of Interactions Among Age, Cultivation Method (Location) and Population on Ginsenoside Content of Wild Panax Quinquefolium L. One Year after Transplanting from Wild
Lim, Wan-Sang ;
Korean Journal of Medicinal Crop Science, volume 13, issue 5, 2005, Pages 254~261
To evaluate the effects of cultivar, environment, age and cultivation times on ginsenoside content among 8 wild populations of American ginseng (Panax quinquefolium), the concentrations of 6 ginsenosides in root were determined at the time of collection (T0) of plants from the wild and 1 year after (T1) transplanting the roots to each of two different forest garden locations. Both location and population had significant effects on root and shoot growth. Overall, ginsenoside Rb1 was most abundant. The second most abundant ginsenoside were Re and Rg1, however the contents of them were not significantly different from each other. Concentrations of Rg1 and Re were inversely related. Ginsenoside Re was influenced by population and location. Ginsenoside Rg1, Rb1, Rc, Rb2 and Rd were influenced by population, location and age. Ginsenoside levels were consistently lower but growth was consistently higher at the more intensively managed garden location.
Genetic Diversity of Wild and Cultivated Populations of American Ginseng (Panax Quinquefolium) from Eastern North America Analyzed by RAPD Markers
Lim, Wan-Sang ;
Korean Journal of Medicinal Crop Science, volume 13, issue 5, 2005, Pages 262~269
The objective of this study was to assess genetic diversity among 6 different wild ginseng populations from New York, Kentucky, North Carolina, Pennsylvania, Tennessee and Virginia, and to compare these wild populations to one cultivated population. RAPD markers were used to estimate the genetic difference among samples from the 7 populations. The 64 random primers were screened, and 15 primers were selected which exhibited the 124 highly reproducible polymorphic markers. The ratio of discordant bands to total bands scored was used to estimate the genetic distance within and among populations. Multidimensional scaling (MDS) of the relation matrix showed distinctive separation between wild and cultivated populations. The MDS result was confirmed using pooled chi-square tests for fragment homogeneity. This study suggests that RAPD markers can be used as population-specific markers for American ginseng.
Expression of a Cu-Zn Superoxide Dismutase Gene in Response to Stresses and Phytohormones in Rehmannia Glutinosa
Park, Myoung-Ryoul ; Ryu, Sang-Soo ; Yoo, Nam-Hee ; Yu, Chang-Yeon ; Yun, Song-Joong ;
Korean Journal of Medicinal Crop Science, volume 13, issue 5, 2005, Pages 270~275
Superoxide dismutases (SOD) are metalloenzymes that convert
. Rehmannia glutinosa is highly tolerant to paraquat-induced oxidative stress. The primary objective of this study was to characterize regulation of SOD gene expression in R. glutinosa in response to oxidative stresses and hormones. A full-length putative SOD clone (RgCu-ZnSOD1) was isolated from the leaf cDNA library of R. glutinosa using an expressed sequence tag clone as a probe. RgCu-ZnSOD1 cDNA is 777 bp in length and contains an open reading frame for a polypeptide consisted of 152 amino acid residues. The deduced amino acid sequence of the clone shows highest sequence similarity to the cytosolic Cu-ZnSODs. The two to three major bands with several minor ones on the Southern blots indicate that RgCu-ZnSOD1 is a member of a small multi-gene family. RgCuZnSOD1 mRNA was constitutively expressed in the leaf, flower and root. The expression of RgCu-ZnSOD1 mRNA was increased about 20% by wounding and paraquat, but decreased over 50% by ethylene and
. This result indicates that the RgCu-ZnSOD1 expression is regulated differentially by different stresses and phytohormones at the transcription level. The RgCu-ZnSOD1 sequence and information on its regulation will be useful in investigating the role of SOD in the paraquat tolerance of R. glutinosa.
Cloning and Characterization of GDP-mannose Pyrophosphorylase from Solanum Tuberosum L.
Hyun, Tae-Kyung ; Lim, Jung-Dae ; Kim, Jae-Kwang ; Seong, Eun-Soo ; Lee, Jae-Geun ; Yoon, Byeong-Sung ; Kim, Myong-Jo ; Cho, Dong-Ha ; Yu, Chang-Yeon ;
Korean Journal of Medicinal Crop Science, volume 13, issue 5, 2005, Pages 276~283
Ascorbic acid is a great antioxidant and helps protect the body against pollutants. GDP-mannose pyrophosphorylase (GMPase) is a key enzyme in manufacturing GDP-mannose, a glycosyl donor for ascorbate and cell wall biosynthesis as well as for protein glycosylation. In this study, we described molecular cloning of a full-length cDNA from Potato (Solanum tuberosum L. cv. Jasim), using tuber. The cDNA isolated encoded a GDP-mannose pyrophosphrylase. The nucleotide sequence of pGMPC showed about 95%, 89% and 80% homology with S. tuberosum (AF022716), N. tabacum (AB066279) and A. thaliana (AF076484) cDNAs clone known as GMPase, respectively. We detected the expression of GMPase using RT-PCR. The highest expression of GMPase was found in stems, and the largest amount of ascorbic acid was also presented in stems. In contrast, the leaf showed minimal level of GMPase transcript and ascorbic acid content. We propose that GMPase expression patterns were similar to the changes of ascorbic acid content in the leaves treated with diverse stresses.
, Phytol and Zingerone
from Chrysanthemum Boreale Makino
Kim, Dong-Hyun ; Bang, Myun-Ho ; Song, Myoung-Chong ; Kim, Soon-Un ; Chang, Young-Jin ; Baek, Nam-In ;
Korean Journal of Medicinal Crop Science, volume 13, issue 5, 2005, Pages 284~287
The flowers of Chrysanthemum boreale Makino were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with n-hexane, EtOAc, n-BuOH and
. Two compounds from the n-hexane fraction and one glucoside from the n-BuOH fraction were isolated through the repeated silica gel and ODS column chromatographies. From the result of physico-chemical data including NMR, MS and IR, the chemical structures of the compounds were determined as
(1), phytol (2) and zingerone
(3). Compounds 2 and 3 were isolated for the first time from this plant.
Molecular Cloning and Characterization of Type 2 Metallothionein cDNA from Codonopsis lanceolata (S. et Z.) Trautv
In, Jun-Gyo ; Lee, Bum-Soo ; Yi, Tae-Hoo ; Yu, Chang-Yeon ; Yang, Deok-Chun ;
Korean Journal of Medicinal Crop Science, volume 13, issue 5, 2005, Pages 288~292
A class I type 2 metallothionein (CMet2) cDNA from taproot of Codonopsis lanceolata was isolated and characterized. A CMet2 cDNA was 572 nucleotides long and had an open reading frame of 234 bp with a deduced amino acid sequence of 78 residues (pI = 4.99). The deduced amino acid sequence of CMet2 matched to the previously reported type 2 metallothionein-like protein genes and showed 74% identity with that of G. max (BAD18377) and C. arietinum (CAA65009). Expression of CMet2 by the RT-PCR was increased at 1 hr after cadmium and hydrogen peroxide treatment, respectively.
Isolation and Characterization of Thioredoxin cDNA from Codonopsis lanceolata (S. et Z.) Trautv
In, Jun-Gyo ; Lee, Bum-Soo ; Rho, Yeong-Deok ; Yu, Chang-Yeon ; Yang, Deok-Chun ;
Korean Journal of Medicinal Crop Science, volume 13, issue 5, 2005, Pages 293~297
A thoredoxin (CTRX) gene was cloned and characterized from a full length cDNA library prepared from taproot of three-year old Codonopsis lanceolata. A CTRX was 666 nucleotides long and has an open reading frame of 372 bp with 124 amino acid residues (pI = 4.92). The deduced amino acid sequence of the CTRX matched to the previously reported plant thioredoxin h genes. The deduced amino acid sequence of CTRX exhibited the similarity of 33-67% among previously registered thioredoxin genes. The expression of CTRX in leaves of Codonopsis lanceolata was increased by wounding and 1 mM
, but decreased by 0.1 mM cadmium.
Root Yields and Saikosaponin Contents Depending on Planting Time and Cultivated Regions of Bupleurum falcatum L.
Kim, Choon-Shik ; Seong, Jae-Duck ; Park, Chung-Heon ; Park, Chun-Gun ; Cho, Yang-Hee ; Kwak, Jun-Soo ;
Korean Journal of Medicinal Crop Science, volume 13, issue 5, 2005, Pages 298~302
In order to decide the optimum planting time for Bupleurum falcatum L. which is grown widely in Korea, some experiments were carried out in different regions such as mid-north area (Gangwon-do, Gyeonggi-do and Chungcheongnamdo), mid-south area (Jeollanam-do, Jeollabuk-do, Gyeongsangnam-do and Gyeongsangbuk-do). The mid-south area had the highest total saikosaponin contents of 1.001, which is the medicinal ingredients of Bupleurum falcatum L., in accordance with the different Bupleurum falcatum L. regions. Also this area has the highest extract contents of 24.3%. Average dry root yields in mid-north area (Gangwon-do) were
kg/ha at planting time of March 20th to March 30th, 500 kg/ha at the time of March 30th to April 10th, and
kg/ha at the time of March 10th to March 20th in Chungcheongnam-do. The optimum planting time in the mid-south area (Jeollabuk-do) was March 10th to March 30th with the average dry root yield of
kg/ha. Average dry root yield in Euiseong, Gyeongsangbuk-do area was 470 kg/ha, and the optimum planting time was April 10th, which suggested that the yield increased as the planting time was delayed. At Jeju-do, the volcanic soil of farsouthern area, average dry root yield was 510 kg/ha at the time of March 10th, but the yield from non-volcanic soil was lower than from volcanic soil, which had a dry root yield of 470 kg/ha.