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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Journal of Medicinal Crop Science
Journal Basic Information
Journal DOI :
The Korean Society of Medicinal Crop Science
Editor in Chief :
Volume & Issues
Volume 13, Issue 6 - Dec 2005
Volume 13, Issue 5 - Oct 2005
Volume 13, Issue 4 - Aug 2005
Volume 13, Issue 3 - Jun 2005
Volume 13, Issue 2 - Apr 2005
Volume 13, Issue 1 - Feb 2005
Selecting the target year
Screening and Extraction Condition of Anti-skin Aging Elastase Inhibitor from Medicinal Plants
Kwak, Yoon-Jin ; Lee, Dae-Hyoung ; Kim, Na-Mi ; Lee, Jong-Soo ;
Korean Journal of Medicinal Crop Science, volume 13, issue 6, 2005, Pages 213~216
To develop a new anti-skin aging cosmetics or functional foods by using elastase inhibitor, a potent elastase inhibitor was screened from various extracts of medicinal plants and its optimal extraction condition was investigated. Methanol extracts of Rubi fructus showed the highest elastase inhibitory activity of 85%. The elastase inhibitor of Rubi fructus was maximally extracted when it was treated with 80% methanol at
for 12hr and its elastase inhibitory activity
was 0.52 mg.
Genotoxicity and Mutagenicity of the Extracts of Morus alba L.
Jin, Hyou-Ju ; Lee, Hyeon-Yong ; Kim, Jong-Dai ; Heo, Moon-Young ; Lee, Jin-Ha ;
Korean Journal of Medicinal Crop Science, volume 13, issue 6, 2005, Pages 217~225
This study was carried out to investigate the genotoxicity in comet and in vitro micronucleus assay and mutagenicity in Ames test of the extracts from leaves and stem of Morus alba L. The samples showed a very weak cytotoxicity on the NIH/3T3 cells by SRB assay. The cell viability of the extracts and fractions from leaves and stems of Morus alba L. was 80% over at
, and that of the chloroform fractions from leaves and stems showed lower than others. The genotoxicity at
of 100% EtOH and water extracts on the NIH/3T3 cells in comet assay was about 40% compared to positive control, and most fractions from 100% EtOH extract of the leaves showed stronger genotoxicity than that offractions from the stem. The genotoxicity with S-9 mix in vitro micronucleus assay of the 100% EtOH and water extracts form Morus alba L. did not indicate any significant difference as compared with control group. The cytokinesis-binucleated cells were showed in the hexan, chloroform, ethylacetate and butanol fractions from the extract of the leaves without S-9, and sample with S-9 showed CB cells in the chloroform fraction from the leaves. In the Ames test, the water and 100% ethanol extracts of Morus alba L. did not have a strong mutagenicity in TA98 and TA100, but the fractions of organic solvents of the ethanol extract had
of mutagenicity on the TA100 strain.
Growth and Fruit Characteristics of Vitis amurensis Rupr. Collected in Gangwondo
Park, Young-Sik ; Heo, Jae-Yun ; Kim, In-Jong ; Heo, Su-Jeong ; Kim, Kyung-Hee ; Jeong, Byung-Chan ; Park, Sung-Min ;
Korean Journal of Medicinal Crop Science, volume 13, issue 6, 2005, Pages 226~233
This study was carried out to evaluate the fruit and growth characteristics of selecting Vitis amurensis through functional material analysis and sensory evaluation in V. amurensis collected in Gangwondo. For evaluation of growth characteristics in V. amurensis, experiments were carried out by compared with the two grape cultivars 'Campbell Early' and 'Kyoho'. The full bloom and verasion time in V. amurensis was investigated faster than those of examined cultivar grapes, while harvest time was investigated latter than those of examined cultivar grapes, but agronomic characteristics was not thought significantly difference between cultivar grapes and V. amurensis. For evaluation of shoot growth phase, the growth curve was very similar to cultivar grapes. The berry size in V. amurensis showed that increases rapidly between 3 and 4 days after full bloom time, and approximately doubles between the second growth period and harvest time, and the berry development phase investigated that consist of two sigmoid growth periods separated by a lag phase. The berry weight and soluble solids in V. amurensis increased with the tree age, but acidity and total sugar contents decreased, and showed a special quality and stable growth according to vine age. To investigation of functional materials, the anthocyanin content in V. amurensis ranged from 16.6 to 50.2 mg/100 g, and the resveratrol content ranged from 0.143 to
which was higher than those of cultivar grapes. These result indicated that V. amurensis tended to have the useful material larger than cultivar grapes. Therefore, other edibility factors of V. amurensis collected in Gangwondo may contribute to breeding studies in Vitis. spp.
Pollen Characteristics of Flower Type and Cross Compatibility with Table Grape in Vitis amurensis Rupr.
Park, Young-Sik ; Kim, In-Jong ; Jeong, Byeong-Chan ; Heo, Jae-Yoon ; Park, Sung-Min ;
Korean Journal of Medicinal Crop Science, volume 13, issue 6, 2005, Pages 234~240
This study was conducted to identify cross compatibility with table grape and pollen characteristics by flower type in Vitis amurensis Rupr. (GW-22, GW-45, GW-56, GW-200, GW-202 and GW-300) collected in Gangwon area. Flower structure, germinability and morphology of pollen, and cross compatibility with table grape were examined. Flowers of GW-22 and GW-56 had an aborted gynoecium and five erect fdaments, and all of them was male. The percentage of pollen germination of GW-22 and GW-56 on the agar medium was 27.6-29.8%. Flowers of GW-45, GW-200, GW-202 and GW-300 had well-developed pistils and five stamens with curled filaments. The percentage of pollen germination of female plant on the agar medium was 0%. Pollen grail)s from GW-22 and GW-56 (male flower) have symmetrically distributed three furrows on their surface and each furrow contains a germ pore at the equatorial level (Tricolporated). GW-45, GW-200, GW202, and GW-300 (Female flower) produced pollen grains with no germinative structures (acolporated). In self-pollina-tion of female flowers, berries of clusters were no berry and no seed. Therefore, flower structure in V. amurensis proved to be that of other dioecious Vitis species. In cross combination of female and male flower of V. amurensis made normal seeds. In cross combination of table grape and V. amurensis of male flower made normal seeds. But, in cross combination of female flower of V. amurensis did not make seeds. Therefore, V. amurensis was cross compatible with vitis spp, and V. amurensis varieties may contribute to breeding studies of Vitis spp.
Comparison of Growth Characteristics, and Extract and Crude Saponin Contents in 4- Year-Old Ginseng Cultured by Direct Seeding and Transplanting Cultivation
Lee,, Sung-Woo ; Cha, Seon-Woo ; Hyun, Dong-Yun ; Kim, Young-Chang ; Kang, Seung-Won ; Seong, Nak-Sul ;
Korean Journal of Medicinal Crop Science, volume 13, issue 6, 2005, Pages 241~244
This study was carried out to compare growth characteristics, and extract and crude saponin contents of 4-year-old ginseng cultured by direct seeding and transplanting cultivation at several farms of the main producing district, Geumsan and Eumsung. Though root weight per plant of direct seeding cultivation was lower than that of transplanting cultivation, but yield of the former was higher than that of the latter owing to high rate of survival plant and Leaf Area Index. Dry matter partitioning ratio of direct seeding cultivation was high in primary root and low in secondary root because direct seeding cultivation elongated the length of primary root, while it suppressed the growth of secondary root. Ratio of rusty root was decreased in condition of direct seeding cultivation, while the contents of extract and crude saponin were lower than that of transplanting cultivation.
Anti-inflammatory Activity of the Ethanol Extract from Magnoliae Flos on PAR2-mediated Edema
Lim, Jong-Pil ; Park, Yeong-Seo ;
Korean Journal of Medicinal Crop Science, volume 13, issue 6, 2005, Pages 245~249
The flower of Magnolia denudata Desrousseaux(Magnoliaceae) has long been used for treatment of nasal disorder in Korea. The physiological activity of the Magnoliae Flos ethanol extract (MFX) was investigated. MFX showed antimicrobial activity. At doses of 100 and 200 mg/kg, MFX showed significant inhibition on both change in paw volume and vascular permeability. MFX (100 mg/kg) significantly inhibited PAR2 agonist-induced myeloperoxidase (MPO) activity in paw tissue. These results indicate that MFX has anti-inflammatory activity in PAR2-mediated paw edema.
Changes in Flavonol Glycoside Contents of Orostachys Japonicus a. Berger according to Cultivation Conditions
Jang, Sang-Hun ; Kang, Dong-Min ; Kang, Jin-Ho ; Park, Jong-Cheol ; Lee, Sang-Gyeong ; Shin, Sung-Chul ;
Korean Journal of Medicinal Crop Science, volume 13, issue 6, 2005, Pages 250~254
The contents of flavonol glycosides,
(5) in the houseleeks controlled by night-break, day-length control, and temperature during overwintering were determined to be compared with those in wild one. The contents of the flavonol glycosides 1-5 in the houseleeks were decreased roughly with warming during overwintering, and increased with longer light duration under the day-length control experiments. While warming functioned negatively on the production of the flavonol glycosides in the houseleek, longer light irradiation did positively during overwintering.
In vitro Antioxidant Activity of Mentha viridis L. and Mentha piperita L.
Lee, Seung-Eun ; Han, Hee-Sun ; Jang, In-Bok ; Kim, Geum-Soog ; Shin, Yu-Su ; Son, Yeong-Deck ; Park, Chung-Berm ; Seong, Nak-Sul ;
Korean Journal of Medicinal Crop Science, volume 13, issue 6, 2005, Pages 255~260
For selecting a new candidate as functional material, this study was conducted on in vitro antioxidant activity and total phenol content of methanol and water extracts prepared from two Mentha species (M viridis L. (M spicata L.) and Mentha piperita L, Extracts of M. viridis showed more efficient scavenging activity on superoxide and DPPH
radical and inhibitory activity on oxidation of human low density lipoprotein (LDL) induced by
and auto-oxidation of linoleic acid than those of M piprita. Methanol extract
and water extract
of M. viridis also exhibited more potent inhibitory activity on LDL oxidation than that of
of final concentration. The total phenol contents of methanol extract and water extract of Mviridis (17.95% & 10.18%, respectively) as tannic acid equivalent were higher than those of M piperita (15.44% & 9.19%). But the yields of methanol and water extracts of M. viridis (13.3 % & 13.5%) were lower than those of M. piperita (14.1 % & 14.6%). The results implies that the extracts from M. viridis (spicata) is more useful material for industrialization as functional food than those from M. piperita.
In vivo Physiological Activity of Mentha viridis L. and Mentha piperita L.
Lee, Seung-Eun ; Han, Hee-Sun ; Jang, In-Bok ; Kim, Geum-Soog ; Seong, Nak-Sul ;
Korean Journal of Medicinal Crop Science, volume 13, issue 6, 2005, Pages 261~267
Alcohol metabolizing and antioxidant activity of Mentha species were investigated in rat liver. Fifty six Sprague Dawley rats were randomly divided into seven groups such as normal (ethanol excluded), negative control (40% ethanol (10 g/kg of body weight/day) fed), positive control (1 g Silymarin/kg of body weight/day with ethanol fed), two Mentha viridis extracts (0.2 g & 1 g M. viridis methanol ext./kg of body weight/day with ethanol fed) and two M piperita extracts (0.2 g & 1 g M. piperita methanol ext./kg of body weight/day with ethanol fed) groups. After 2 weeks, rats were sacrificed under ether. The activities of alcohol dehydrogenase (ADH), aldehyde dehydrogenase (ALDH), catalase (CAT), manganese superoxide dismutase (Mn-SOD), glutathione peroxidase (GAH-px) and the content ofthiobarbituric acid reactive substance (TBARS) in the rat livers and the activity of glutamate pyruvate transferase (GPT) in serum were evaluated. From the analyses, 1 g M. viridis and 0.2 g M. piperita administrated groups showed higher ADH and ALDH activity than the other groups. Groups fed with 0.2 g and 1 g M. viridis ext. and 0.2 g M. piperita ext. showed higher CAT activity than the other groups. All the Mentha extract fed groups exhibited more effective in recovering Mn-SOD, GSH-px and GPT acitivities to a similar degree of normal group. TBARS contents of two M. viridis ext. fed group and 0.2 g M. piperita ext. fed group were higher than those of the other groups. M. viridis extract fed groups showed more effective in CAT and Mn-SOD activities than M. piperita extract groups at p < 0.05. Finally, it is concluded that both Mentha species have alcohol metabolizing and antioxidant activity and M viridis is more effective than M. piperita.
Effects of Nonsupporting Methods on Growth and Yield of Cynanchum auriculatum Royle ex Wight
Kim, Min-Ja ; Song, Beom-Heon ; Nam, Sang-Young ; Kim, In-Jae ; Lee, Cheol-Hee ; Yun, Tae ;
Korean Journal of Medicinal Crop Science, volume 13, issue 6, 2005, Pages 268~272
This study was carried out to investigate the labor saving cultivation of C. auriculatum, which has much bigger root than local variety C. wilfordii, and tested three nonsupporting methods, i.e., untreated control, top pinching, and cutting of branch-top compared with I type stake. Top growth was more vigorous in staking cultivation than in nonsupporting cultivations. Among nonsupporting methods, main vine length and total length of main vines per plant were increased in order of untreated control > top pinching > cutting of branch-top, while the opposite is true to diameter of main vein. Number of leaves per plant was lower, but leaf length and leaf width were higher in cutting of branch-top than in other nonsupporting methods. Number of branched root and diameter of main root were bigger in cutting of branch-top and in staking cultivation, while length of main root and diameter of branched root were greater in staking cultivation and in top pinching than in other treatments. Although yield of marketable root per 10a was
lower, income was
higher in nonsupporting methods than in staking cultivation by cost saving to staking installation. Among nonsupporting methods, yield of marketable root and income were 6% and 8% higher in cutting of branch-top than in untreated control, respectively.
Efficient Procedures for Direct Shoots Regeneration from Leaf Explants of Rehmania glutinosa Lib.
Hwang, Sung-Jin ;
Korean Journal of Medicinal Crop Science, volume 13, issue 6, 2005, Pages 273~277
Adventitious shoots were directly induced from leaf explants of R. glutinosa, an important medicinal plant. Proliferating shoot cultures were obtained by culturing leaf discs on Murashige and Skoog(MS) medium alone or combination with auxins and cytokinins. MS medium supplemented with 1
IAA was the most effective, providing high shoot bud formation frequency without formation of intervening callus. The effect of leaf age on adventitious shoot formation was also investigated. The maximum shoot bud production (93.4%) was achived using 3rd leaf from apex of 6 weeks old plantlets after seed germination. Plantlet were rooted on an half-strength MS (1/2MS) medium containing 0.1
. This prtocol is useful for clonal propagation and Agrobacterium-mediated transforamtion in R. glutinosa.
Classification of Artemisia spp. Collections Based on Morphological Characters and RAPD Analysis
Park, Sang-Kyu ; Chung, Bong-Hwan ; Kim, Hong-Sig ; Cho, Yong-Gu ;
Korean Journal of Medicinal Crop Science, volume 13, issue 6, 2005, Pages 278~286
This study was conducted to classify 80 Artemisia spp. collections based on morphological characters and RAPD analysis to get the basic information of Artemisia spp. collections as medicinal plants. The eighty Artemisia spp. collections were classified into 5 groups with the maximum distance 0.82 between clusters based on the complete linkage cluster analysis with morphological traits. Out of 80 operon primer, 10 primers showing polymorphic bands were selected for RAPD analysis. Among the 98 bands amplified with the primers, 68 (69%) bands showed polymorphism. The number of amplified bands ranged from 8 to 10 with an average number of 9.8 bands. Artemisia spp. collections classified into 6 groups with the similarity value of 0.63 in dendrogram derived from the cluster analysis based on RAPDs. Group consisted of 29 collections. Group, which is the largest one, contained 40 collections. Most of the A. asiatica and A. feddei LEV et VNT. were classified into Group and. The rest of the collections (31%) were classified into Group
Effects of Covering Materials on Prevention of Freeze Damage and Labor Saving in Saururus chinensis Baill During Wintering
Nam, Sang-Young ; Kim, In-Jae ; Kim, Min-Ja ; Yun, Tae ; Lee, Cheol-Hee ; Park, Sung-Gue ; Lee, Woo-Young ; Kim, Hong-Sig ;
Korean Journal of Medicinal Crop Science, volume 13, issue 6, 2005, Pages 287~292
To investigate the effects of heat conservation materials on freeze damage and weed occurrence during overwintering in Saururus chinensis, Temperature difference was
in chaff, and was lower than those ranged from 9.7 to
in other materials. Heat conservation index, calculated from average temperatures below
of earth's surface during the winter, was higher
in chaff and lagging, respectively than in straw. Preservation of water is greater
in covering than in open field, and it increased in the order of lagging > chaff > straw among heat conservation materials. The survival rate of rhizome was increased in the order of 99% in lagging > 75% in chaff > 58% in straw, 32% in open field after overwintering, budbreak began fast, and the numbers of total budbreak per unit area were greater 22 times in lagging than 35.0 units in open field. Weeds occurrence was decreased in covering, i.e.,
of dry weight, and
of labor input compared with
of dry weight, and 65.7 hours/10a of labor input in open field. Growth of top part was better in covering than in open field, and the number of tillers per unit area showed
compared with 293 in open field. Marketable yields of dried stem and leaves and rhizome were higher
, respectively in covering than in open field, and among heat conservation materials, those were highest in lagging.
Effects of the Fermentation Periods on the Qualities and Functionality of the Vegetable Fermentation Broths
Kim, Na-Mi ; Lee, Jong-Won ; Do, Jae-Ho ; Park, Chae-Kyu ; Yang, Jae-Won ;
Korean Journal of Medicinal Crop Science, volume 13, issue 6, 2005, Pages 293~299
To determine optimal fermentation period of vegetables mixed with black sugar without innoculation of microorganisms, changes in chemical components, quality characteristics of the fermented broth and physiological functionality during the fermentation period were investigated, pH and
of the fermented broths were decreased gradually during the fermentation period. Except for radish, L and a color values of fermented broths were increased but b values were decreased during fermentation period. Viscosity of fermented broths of vegetables were decreased after 3 months of fermentation.
activity in fermentation broth of brocolli, eggplant, cabbage, chicory, aralia, radish were increased to 460, 430, 180, 420, 560, 260 after six months fermentation period. In radish and tomato fermentation broth, invertase activity were increased to 200 and 460 units and cellulase activity were increased to 280 and 140 after six months fermentation period. The content of total phenolic compounds and electron donating ability were the highest after 2 to 4 months fermentation period and decreased thereafter. No significant level of tyrosinase inhibitory activity and SOD-like activity were observed. In the sensory evaluation test of aralia fermentation broth of droop, sweet and sour flavor and bitter, astringent taste were decreased during the fermentation period and droop tastes were highest in 3 months. In radish fermentation broth, radish flavor and pungent taste were decreased and sweet taste was increased during fermentation period. Acceptability in overall was the greatest after three months. Based on the results stated above, optimal fermentation period was appropriated 3 or 4 months.
In vitro propagation from axillary bud explants of Fatsia japonica Deene. et Planeh
Choi, Kyung-Mi ; Hwang, Sung-Jin ; Ahn, Jun-Cheul ; Lee, Hyeon-Yong ; Kim, Jae-Heun ; Hwang, Baik ;
Korean Journal of Medicinal Crop Science, volume 13, issue 6, 2005, Pages 300~303
A propagation for Fatsia japonica using axillary bud explants were established. Cultures were initiated from axillary bud explants on MS medium supplemented with IAA
in combination with BA
. The maximum shoot bud formation was obtained in MS medium supplemented with
IAA after 4 weeks culture. The microshoot rooted within 4 week in MS medium containing