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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Medicinal Crop Science
Journal Basic Information
Journal DOI :
The Korean Society of Medicinal Crop Science
Editor in Chief :
Volume & Issues
Volume 14, Issue 6 - Dec 2006
Volume 14, Issue 5 - Oct 2006
Volume 14, Issue 4 - Aug 2006
Volume 14, Issue 3 - Jun 2006
Volume 14, Issue 2 - Apr 2006
Volume 14, Issue 1 - Feb 2006
Selecting the target year
Fibrinolytic Enzyme Activity of Extract from Camellia japonica L.
Lim, Chae-Young ; Lee, Sook-Young ; Pyo, Byeong-Sik ; Kim, Sun-Min ;
Korean Journal of Medicinal Crop Science, volume 14, issue 4, 2006, Pages 195~201
The fibrinolytic activities of soluble proteins extracted from young leaves of Camellia japonica L. were studied. Fibrinolvity activity of extract from partitions of C. japonica L. showed 1.6-2.0 times higher than plasmin used as positive control. The fibrinolytic enzyme was confirmed directly from young leaves of C. japonica L. by a fibrin Plate and fibrin zymography. The protein was composed of a single polypeptide and its apparent molecular weight was found to be 45 kDa, as judged by SDS-polyacrylamide gel electrophoresis. The optimum pH and temperature for the fibrinolytic activity were pH 5.5 and
, respectively. Also, the fibrinolytic activity was clearly inhibited by PMSF and TLCK, suggesting that it is a member of the trypsin-like serine protease. All these results suggest the protease is a fibrinolytic enzyme belong to a family of trypsin-like serine protease.
Isolation of Antagonistic Bacteria against Major Diseases in Panax ginseng C.A. Meyer
Chung, Ki-Chae ; Kim, Chang-Bae ; Kim, Dong-Ki ; Kim, Bok-Jin ;
Korean Journal of Medicinal Crop Science, volume 14, issue 4, 2006, Pages 202~205
Ginseng is major medicinal plant in Korea. Because of its long cultivation period the yield losses of 5 years of ginseng is 50% due to various diseases. The objective of this study is to select potential biocontrol agents. As the result of research so far achieved to contribute to rational prevention of ginseag plant disease for the stable cultivation of ginseng, three bacterial strains, Streptomyces lauretii strain B8180, Bacillus subtilis strain 8856, and Burkholderia cepacia strain 7944 were isolated from oak leaf compost. The strains showed antagonistic activities against five ginseng pathogenic fungi (Cylindrocarpon destructans, Rhizoctonia solani, Phytophthora cactorum, Botrytis cinerea, Fusarium solani f. sp. panacis) and control effects on Phytophthora blight.
Effect of Aqueous Extracts from Rubus coreanus Miquel and Angelica gigas Nakai on Anti-tumor and Anti-stress activities in mice
Kim, Jung-Hwa ; Kim, Cheol-Hee ; Kim, Hyou-Sung ; Kwon, Min-Chul ; Song, Young-Kyu ; Seong, Nak-Sul ; Lee, Seung-Eun ; Yi, Jae-Seon ; Kwon, Oh-Woung ; Lee, Hyeon-Yong ;
Korean Journal of Medicinal Crop Science, volume 14, issue 4, 2006, Pages 206~211
This study was performed to examine antitumor activities of Rubus coreanus Miquel and Angelica gigas Nakai extracts against sarcoma-180 and anti-stress activities in ICR mice. The variation of body weights of the 20 days of Rubus coreanus extracts-administrated mice group was very low. The survival rate (T/C %) of Rubus coreanus extract administrated group was 161% after 50 days from the inoculation of sarcoma-180 and the increment of their body weights was suppressed. Anti-stress effect of the extracts of R. coreanus and A. gigas were estimated by maeasuring blood chemical value and internal organs weight in ICR mice. The extracts of R. coreanus reduced the cholesterol and glucose to the normal level in the all stress animal models. The extracts of R. coreanus reduced the hypertrophy of the internal organs such as adrenal, spleen and liver to the regular level.
Isolation of Antioxidative Compound from Scutellaria baicalensis G.
Kim, Seok-Chang ; Ahn, Kun-Seok ; Park, Chae-Kyu ; Jeon, Byeong-Seon ; Lee, Jong-Tae ; Park, Won-Jong ;
Korean Journal of Medicinal Crop Science, volume 14, issue 4, 2006, Pages 212~216
Root of Scutellaria baicalensis G. was extracted with methanol and water to give the yield of 30.0% in order to find the antioxidant substance. The extract was fractionated with diethyl ether, n-butanol and water to test the inhibitive activity against xanthine oxidase. Three fractions inhibited the activities of xanthine oxidase by 48.2%, 10.2% and 2.8%, respectively, at the amount of
. A component that exhibited strong inhibition of xanthine oxidase was isolated from diethyl ether fraction (SE Fr.) by silica gel column chromatography and HPLC, and then identified by
and MS spectrophotometry. EDA (Electron Donating ability) of the compound was 28.5% at the concentration
. That was identified to be 3,5,7-trihydroxy-2'-methoxyflavanone by spectrophotometric analysis using
and Mass spectrophotometry.
Effects of Insects on Pollination in Angelica gigas Nakai and Angelica acutiloba Kitagawa
Kim, Dong-Hwi ; Park, Hee-Woon ; Park, Chun-Geun ; Sung, Jung-Sook ; Seong, Nak-Sul ;
Korean Journal of Medicinal Crop Science, volume 14, issue 4, 2006, Pages 217~220
It is known that the process of variety development and maintenance of original plant is difficult in Angelica gigas and Angelica acutiloba because they are allogamous plants. The contents like this are stand in the way of theirs breeding. This study was conducted to investigate the foraging activity and pollinational efficacy by insects to flowers of A. gigas and A. acutiloba. Foraging activity by pollinators was observed every day from 10:00 to 10:30 during 3 days after September 21. Honey bee and fly were major pollinators. The rate of hymenoptera and diptera to total numbers of insects was the 56.5% and 40.6% in A. gigas, respectively. It was also 46.3% and 51.8% in A. acutiloba, respectively. The other insects were lepidoptera, coleoptera and hemiptera. The numbers of hymenoptera and diptera were more in flowers of A. gigas was 49.2% with bee, 36.0% with fly, 15.3% with human, 0.9% without anything in the nethouse and 76.6% in the open field. That of A. acutiloba was 43.6% with bee, 27.9% with fly, 20.3% with human, 0.6% without anything in the nethouse and 46.9% in the open field. Excepting open field, seed setting rate of with bee in the nethouse was the highest of all the others. However, we concluded that the use of fly was the most effective method for pollination of A. gigas and A. acutiloba considering low cost and convenient handling.
Effect of Furrow Directions on Growth and Yield in Panax ginseng C.A. Meyer
Lee, Sung-Woo ; Cha, Seon-Woo ; Hyun, Dong-Yun ; Kim, Young-Chang ; Kang, Seung-Won ; Seong, Nak-Sul ;
Korean Journal of Medicinal Crop Science, volume 14, issue 4, 2006, Pages 221~224
To analyze how the furrow directions made by the azimuth of
effect on the growth characteristics and yield of 2 and 3-year-old ginseng, both of
recommended as the standard practices for ginseng cultivation.
showed lower quantum and air temperature during forenoon, while it showed higher quantum and air temperature from 15:00 to 19:00 than that of
showed high quantum and air temperature due to the distinct increase of sunlight penetrated from 9:00 to 11:00, and which were similar to that of
during afternoon. Stem length and leaf area in
, respectively, were decreased more than that of
, and those in
were decreased more distinctly than
. Heat injury rate was decreased in
, while it was increased distinctly in
that showed high quantum and air temperature, and which showed great variation according to the year. Yield of
was decreased more than that of
in 2004, the year when it was a relatively high air temperature during summer season, but which was increased more than that of
in 2005 showing a rotatively low air temperature.
Effects of Plant Growth Regulation on Adventitious Root Formation of Pulsatilla koreana Nakai
Yoon, Eui-Soo ; Kwon, Hye-Kyoung ; Cho, Yi-Yun ;
Korean Journal of Medicinal Crop Science, volume 14, issue 4, 2006, Pages 225~228
To acquire the normal regeneration of plantlets, we investigated combinations and concentrations of plant growth regulations for optimal conditions of adventitious root formation. Based on the previous study, we performed callus and shoot induction. When induced shoot was transferred into a rooting medium containing plant hormones, it wilted and died. Thus, the shoot proliferated on 1/2 MS medium for 10 days and was then treated with MS medium supplemented with 3.0 mg/L NAA for 3 days. Adventitious root formations were observed after shoot planlets were transferred to 1/3 MS medium. The concentrations of salt and sucrose were gradually reduced in MS medium and the rooted plantlets were transferred for acclimatization into a mixture of peatmoos : perlite (3 : 2).
Comparison of Grade of Raw and Red Ginseng on each Factor of Quality in Korean and American Ginseng
Chung, Chan-Moon ; Shin, Ju-Sik ;
Korean Journal of Medicinal Crop Science, volume 14, issue 4, 2006, Pages 229~233
Comparison of the grade of raw ginseng and that of red ginseng was investigated. The materials used in this study were Korean ginseng (Panax ginseng C. A. Meyer) and American ginseng(Panax quinquefolium L.) Coefficient of body term, length of main stem and weight of raw ginseng were used as the classifying criteria of the root size and grades. Korean ginseng distinguished the distribution of weight size from that of American ginseng. Korean ginseng distributed largely in middle and large root size, and American ginseng distributed largely in middle and small root size. American ginseng had shorter length of main root, bigger diameter of main root and more number of adventitious roots than Korean ginseng. The quality of Korean ginseng was better than that of American ginseng. In Korean ginseng, high quality of red ginseng above second grade (Jisam) was obtained, but low quality of red ginseng under third grade (Yangsam) in American ginseng. In Korean raw ginseng, the coefficients of body form of middle weight and large weight size were under 0.5, but those of American ginseng was over 0.5. So American ginseng were not adequate to produce good red ginseng. Those factors as length of main root and weight of main root were not significantly influenced on the qualify of red ginseng in both Korean ginseng and American ginseng. Coefficient of body form was leading factor affecting the quality of red ginseng. To improve the quality of red ginseng, coefficient of body form, weight of main root and length of main root were controlled adequately in both Korean ginseng and American ginseng.
Total Phenol Contents and Antioxidant Activity in Orostachys japonicus A. Berger Grown under various Cultivation Conditions
Yu, Eun-Ae ; Lee, Sung-Joong ; Lee, Sang-Gyeong ; Kang, Jin-Ho ; Shin, Sung-Chul ;
Korean Journal of Medicinal Crop Science, volume 14, issue 4, 2006, Pages 234~238
Changes in the contents of total phenolic compounds in as Orostachys japonicus well as their antioxidant capacity according to the control of night-break, day-length control, variation in luminosity and amount of fertilizer used were examined. The results were compared with those obtained from wild Orostachys japonicus. The contents of the total phenolic compounds in Orostachys japonicus were roughly proportional to the increasing level of irradiation and nitrogen fertilization. The total content of phenolic compounds in the wild Orostachys japonicus of was higher than cultivated under various conditions. The antioxidant capacities of the Orostachys japonicus were increased roughly with increasing level of irradiation and nitrogen fertilization. Overall, the total content of phenolic compounds of Orostachys japonicus and their antioxidant capacity were not significantly affected by the amount of phosphorus and potassium fertilizer used.
Shading Effect on Growth and Flowering of Orostachys japonicus A. Berger
Hong, Dong-Oh ; Lee, Chang-Woo ; Kim, Hong-Young ; Kang, Jin-Ho ; Ryu, Yeong-Seop ; Shin, Sung-Chul ;
Korean Journal of Medicinal Crop Science, volume 14, issue 4, 2006, Pages 239~243
Orostachys japonicus, a monocarpic plant, is artificially grown in greenhouses. The study was carried out to examine the effect of shading (0, 35, 55, 75, 95%) on growth, morphological characters, and flowering. The treatments were done on August 25 and afterward samples were taken every 2 weeks until October 20, in which growth, morphological and flowering related characters were measured. With severer shading plant height, inflorescence length, number of leaves including bracts and stem diameter were decreased although severely declined in 95% shading treatment. Leaves and bracts, stem, root, shoot and total dry weights increasingly declined with severer shading. Florets formed on the inflorescence showed similar response to the shading treatment as plant height did. Flowering of the florets was not observed throughout all the sampling period, meaning that different light intensities by shading did not affect their flowering.
Effects of Light Intensity on Photosynthesis and Growth in Seedling of Kalopanax pictus Nakai
Lee, Cheul-Ho ; Shin, Chang-Ho ; Kim, Kyu-Sick ; Choi, Myung-Suk ;
Korean Journal of Medicinal Crop Science, volume 14, issue 4, 2006, Pages 244~249
This study was carried out to determine leaf photosynthetic capacity and growth characteristics of Kalopanax picturs Nakai seedlings under the different light condition. The seedlings grown under low light condition showed larger leaf area and petiole, and higher relative growth rate than those grown under high light condition. Chlorophyll contents as chlorophyll a, chlorophyll b, and chlorophyll a + b were high in the seedlings grown under highlight condition compared to those grown in low light conditions. The mean absorption value of shade leaf within photosynthetically active radiation (400-700 nm) was slightly higher than that of sun leaf, Leaf photosynthetic capacity of seedling was variable under the different light conditions. Seedling grown under high light condition had the higher photosynthetic capacity. Leaf photosynthetic rates under forestry and nursery were 700 and
, respectively. However, leaf photosynthetic rates under high and low light conditions were 500 and
Cytogenetic Analyses of Astragalus Species
Kim, Soo-Young ; Choi, Hae-Woon ; Kim, Chan-Soo ; Sung, Jung-Sook ; Lee, Joong-Ku ; Bang, Jae-Wook ;
Korean Journal of Medicinal Crop Science, volume 14, issue 4, 2006, Pages 250~254
To elucidate cytogenetic differences, karyotype analysis and FISH (fluorescence in situ hybridization) with 45S and 5S rDNAs were carried out in the three Astragalas species: Astragalas membranaceus Bunge, A. membranaceus var. alpinus Nakai and A. mongholicus Bunge. The somatic metaphase chromosome numbers of all three species were 2n=2x=16 and the size of chromosomes ranged
. The chromosome complement of A. membranaceus consisted of each four pairs of metacentrics (chromosomes 3,4,6 and 7) and submetacentrics (chromosomes 1,2,4 and 8). In A. membranaceus var. alpinus, the chromosome complement consisted of two pairs of metacentrics (chromosomes 4 and 8) and six pairs of submetacentrics (chromosomes 1,2,3,5,6 and 7). A. mongholicus had three pairs of metacentrics (chromosomes 6,7 and 8) and five pairs of submetacentrics (chromosomes 1,2,3,4 and 5). Using bicolor-FISH, one pair of 45S and 5S rDNA signals could be detected on the centromeric regions of chromosomes 8 and 7 of A. membranaceus and A. mongholicus, respectively. In contrast, A, membranaceus var. alpinus had one pair of 45S signals on the centromeric region of chromosome 8 and two pairs of 5S rDNA signals on the short arms of chromosomes 7 and 8.
Somatic Embryogenesis and Plant Regeneration in Embryogenic Cell Suspension Cultures of Hovenia dulcis Thunb
Li, Cheng-Hao ; Zhao, Bo ; Kim, Na-Young ; Kim, Myong-Jo ; Cho, Dong-Ha ; Lee, Dong-Wook ; Lee, Jae-Geun ; Lim, Jung-Dae ; Yu, Chang-Yeon ;
Korean Journal of Medicinal Crop Science, volume 14, issue 4, 2006, Pages 255~260
Culture conditions for high frequency plant regeneration via somatic embryogenesis from embryogenic cell suspension cultures of Hovenia dulcis are described. Germinated somatic embryos were selected for induction of secondary embryogenesis. Friable embryogenic cells were induced directly from somatic embryos when transfer to 1/3 MS solid or liquid medium lacking plant growth regulators. The temperature strongly effected on induction of secondary embryognesis than other conditions in culture. All somatic embryos produced friable embryogenic cell clumps within 10 days when germinated somatic embryos cultured in 1/3 MS medium at
in suspension culture. No somatic embryos formed from embryogenic cell suspension cultures at
. Numerous somatic embryos were induced and subsequently developed uniformly into germination stage from suspended cell clumps after 4 weeks of culture on
. Plantlets conversion were observed on
when germinated somatic embryos were transferred to 1/3 MS solid medium without plant growth regulators or supplemented with 0.1-0.5 mg/l benzyladenine.