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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Medicinal Crop Science
Journal Basic Information
Journal DOI :
The Korean Society of Medicinal Crop Science
Editor in Chief :
Volume & Issues
Volume 15, Issue 6 - Dec 2007
Volume 15, Issue 5 - Oct 2007
Volume 15, Issue 4 - Aug 2007
Volume 15, Issue 3 - Jun 2007
Volume 15, Issue 2 - Apr 2007
Volume 15, Issue 1 - Feb 2007
Selecting the target year
Development of SCAR Marker for Discriminating between Violet Flowered Lines and White Flowered Lines in Chinese Bellflower (Platycodon grandiflorum A.)
Park, Chun-Geon ; Bang, Kyong-Hwan ; Kim, Ok-Tae ; Jin, Dong-Chun ; Kim, Dong-Hwi ; Sung, Jung-Sook ; Seong, Nak-Sul ; Park, Hee-Woon ; Lee, Sang-Chul ;
Korean Journal of Medicinal Crop Science, volume 15, issue 1, 2007, Pages 1~5
To develop a convenient method for discriminating between violet flowered lines and white flowered lines in Chinese bellflower, RAPD analysis was carried out and SCAR markers were generated. Eighteen specific RAPD bands were obtained from 6 OPERON primer sets. Two out of eighteen RAPD bands were cloned into pGEM-T-Easy vectors and then subjected to the nucleotide sequence analysis. PgR1 and PgR2 DNA fragment, each specific for violet and white flowered lines, consist of 887 bp and 863 bp sequences, respectively. Two SCAR markers were developed from RAPD clones: SPgR1 (355 bp) from PgR1 and SPgR2 (493 bp) from PgR2. One (SPgR2) of these two markers was useful to differentiate between violet flowered lines and white flowered lines in Chinese bellflower.
Isolated and Identification of Biological Activity Compounds from Leaves and Stem of Paeonia lactiflora Pallas
Kim, Se-Jong ; Park, Jun-Hong ; Choi, Seong-Yong ; Son, Kun-Ho ; Kim, Kil-Ung ;
Korean Journal of Medicinal Crop Science, volume 15, issue 1, 2007, Pages 6~11
This study was conducted to identify physiologically active substances from leaves and stems of peony plant. MeOH extracts and column chromatography were employed to isolate active compounds and chemical structure were identified by IR, UV, Mass and NMR. The results obtained can be summerized as followings : Chemical structure of compound 1 was identified as oleanolic acid (white color form) of triterpenoid group, which is firstly identified from the above part of peony. Compound 2 was identified as kaempferol (yellow needle form) of flavonoid group, which was firstly identified from the root, leaf and stem of peony. Compound 3 was identified as methyl gallate (white power form) of phenol group, which was firstly identified from the above part of peony. Compound 4 was identified as astragalin (bright yellow needle form) of flavonoid group, that was firstly identified from the leaf and stem of peony. Compound 5 was identified as paeoniflorin (white color form) of monoterpene group, that was firstly identified from the above part of peony.
Soil Properties and Plant Yield in the Cultivation Area of Ostericum Koreanum Kit.
Hur, Bong-Koo ; Kim, Chan-Yong ; Son, Seong-Gon ; Oh, O-Jin ;
Korean Journal of Medicinal Crop Science, volume 15, issue 1, 2007, Pages 12~16
This study was carried out to investigate the cultivated soil properties, the growth and yield of Ostericum koreanum Kit. in the Bonghwa area. The results were as follows: In Bonghwa area, soil texture of upland soil were mostly sandy loam and loam. Sloped areas of
were plentiful than the other slopes, that of next was
slope. Sandy loam and loam soil were mostly 'well' in the drainage class, but loamy coarse sand and loamy sand soil were 'excessively well' drainage class. In the 'moderately well' drainage class, yield of Ostericum koreanum was 289kg/10a, and that of 'poorly' drainage class was low. The yield of loam soil texture was 284kg/10a. Soil organic matter, Potassium and EC were lower in 'poorly' drainage, but the other constituents were not definite tendency by different drainage classes.
Effects of Various Composts and NPK Fertilizers Application to the Yacon (Polymnia sonchifolia POEPP) Growth
Hur, Bong-Koo ; Choung, Nack-Hyun ; Kim, Zhoo-Hyeon ; Oh, O-Jin ; Son, Seong-Gon ; Kang, Dae-Yong ;
Korean Journal of Medicinal Crop Science, volume 15, issue 1, 2007, Pages 17~20
This study was conducted to investigate the effect of various composts and NPK fertilization rates on the growth of yacon (Polymnia sonchifolia POEPP). Fertilization of used soil was low. Mean temperature of July was higher than that of normal year, but those of another months were same or the lower. The yield of the no-compost plot was higher by 74% than the no-fertilization plot. Increased NPK fertilization rates of bark compost and organic fertilizer plots increased the growth and yield of yacon. The yield of bark compost 1.5 times plot was highest by 6,905 ㎏/10a. The chemical contents of leaves in the various compost and NPK fertilization rates were not normal tendency, and also that of no-compost plot was not difference to the NPK fertilization rates plot.
Hepatoprotective Effect of Catechin Isolated from the Root of Rosa rugosa Thunb
Hur, Jong-Moon ; Kim, In-Ho ; Park, Jong-Cheol ;
Korean Journal of Medicinal Crop Science, volume 15, issue 1, 2007, Pages 21~25
The root of Rosa rugosa has been used in folkloric medicine as a treatment agent for diabetes. In the present study, we investigated whether (+)-catechin isolated from this plant can change the activities of hepatic drug metabolizing enzymes in rats treated with bromobenzene. Pretreatment with (+)-catechin gave no effects on the activities of aminopyrine N-demethylase and aniline hydroxylase, enzymes forming toxic bromobenzene epoxide intermediates and glutathione Stransferase, an enzyme removing toxic epoxides. However, the activity of epoxide hydrolase, an enzyme detoxifying the bromobenzene toxic intermediates was mildly recovered by (+)-catechin treatment.
Search for Medicinal Plants on Improvable Effect of Intestinal Microflora
Cho, In-Sook ; Han, Young-Hee ; Lee, Gee-Young ; Park, Kyeong-Yeol ;
Korean Journal of Medicinal Crop Science, volume 15, issue 1, 2007, Pages 26~29
Extracts of 13 medicinal plants were tested to investigate the improvable effects on microflora growth in the intestines of human. Water extracts of Artemisia capillaris, Taraxacum platycarpum and Ethanol extracts of Allium tuberosum, Artemisia capillaris, Artemisia vulgaris, Plantago asiatica, Schizandra chinensis, Sedum sarmentosum, Taraxacum platycarpum, Zingiber officinale inhibited the growth of C. perfringens. Water and ethanol extracts of Sedum sarmentosum, Schizandra chinensis inhibited the growth of E. coli. While 13 medicinal plants were not remarkably inhibited the growth of B. longum. Therefore, ethanol extracts of Schizandra chinensis, Sedum sarmentosum can be utilized as functional food materials to improve in the intestines.
Effect of Korean Mistletoe Extract M11C (Non-lectin Components) on the
Expression and Secretion from Human Peripheral Blood Monocytes
Jun, Myung-Ha ; Kang, Tae-Bong ; Chang, Sung-Ho ; Choi, Wahn-Soo ; Seong, Nak-Sul ; Her, Erk ;
Korean Journal of Medicinal Crop Science, volume 15, issue 1, 2007, Pages 30~37
It is well-known that Korean mistletoe (Viscum album) extract has an immune activity and anticancer effect. In this study, Korean mistletoe extract, M11C (non-lectin components), was used to examine whether this extract might activate human peripheral monocyte to produce tumor necrosis
. To examine the effect of M11C on the production of
from monocyte, the monocyte were stimulated by the M11C, and then collected the supernatant (M11C stimulated monocyte-conditioned media; MCM). MCM was treated to the
sensitive L929 cells, and then L929 cytotoxicity was measured by means of MTT. MCM had cytotoxic effect on L929. And the cytotoxic effect of MCM on L929 was almost abolished by
antibody. These data indicated that MCM contained
, suggesting the
generation from M11C-stimulated monocyte. This suggestion was confirmed from the data that
was highly detected in MCM by immunoblotting technique. M11C effect on
production from monocyte was in the dose and stimulating time dependent manners. Also the effect of M11C on the expression of
mRNA from monocyte was shown in the dose and stimulating time dependent manners. As a result, Korean mistletoe extract, M11C, could be used for an immunostimulator.
Effect of M11C (Non-lectin Components) Obtained from Korean Mistletoe on the
Secretion from Mouse Splenocytes
Jun, Myung-Ha ; Kang, Tae-Bong ; Chang, Sung-Ho ; Choi, Wahn-Soo ; Seong, Nak-Sul ; Her, Erk ;
Korean Journal of Medicinal Crop Science, volume 15, issue 1, 2007, Pages 38~45
Korean mistletoe (Viscum album L) extract has been found to posses immunoregulating activity. In this study, Korean mistletoe extract, M11C (non-lectin components), was used to know whether this extract activates splenocytes to secret interleukin
. The splenocytes were treated with M11C, and then collected the supernatant and cell lysate that were prepared to analyze the level of
, using ELISA, immunoblotting, and RT-PCR. Maximum effective dose and time of M11C on
secretion from splenocytes were
and 8 hours, respectively. Treatment dose and time for the maximum expression of
and 4 hours, respectively. Saccharide degradation enzyme Viscozyme L completely blocked the effect of M11C on
secretion from splenocytes. As the result, among non-lectin components saccharide could be regarded as a main component for
expression from splenocytes.
Optimal Culture Conditions for Transformed Root Growth and Trichosanthin Formation in Trichosanthes kirilowii Max.
Hwang, Sung-Jin ; Na, Myung-Suk ;
Korean Journal of Medicinal Crop Science, volume 15, issue 1, 2007, Pages 46~50
Transformed hairy roots were induced from in vitro grown plantlets of Trichosanthes kirilowii by infection with Agrobacterium rhizogenes strain ATCC15834. Transformed hairy roots exhibited active growth with high branching of roots on plant growth regulators-free medium. Cloned line (TR-03) of hairy root was tested for its growth and extracellular protein accumulation in medium under various culture conditions. Among the culture media tested, a full-strength MS medium had a pronounced effect on root biomass and extracelluar protein accumulation in medium. The maximum root biomass (2.4 g DRW/flask) and extracellular total protein contents
in medium was obtained at inoculum size of 2 g (FRW) and in MS medium supplemented with 4% sucrose. In addition, the optimal shaking speed for root growth and extracellular protein accumulation in medium were 100 rpm. The total extracellualr protein concentration reached a maximum of
at 4 weeks and decreased thereafter. Protein translation inhibitory activity was observed in culture broths and reached levels of 21.3 unit. These studies demonstrate that the transformed hairy roots can be utilized for the in vitro production of ribosome-inactivating proteins.
Antioxidant Activity of Momordica charantia L. Extracts
Park, Yeol ; Boo, Hee-Ock ; Park, Young-Lan ; Cho, Dong-Ha ; Lee, Hyun-Hwa ;
Korean Journal of Medicinal Crop Science, volume 15, issue 1, 2007, Pages 56~61
This study was carried out to determine the antioxidant activity and vitamin C contents in plant extracts of the Momordica charantia L. The vitamin C was detected as the highest content in immature fruit (92.2 mg/100 g), while the content in stem (2.5 mg/100 g) was lower 40 times than that of immature fruit. Antioxidant activity for the dried sample was investigated by TBA method. The lowest TBARS values were obtained from extracts of dried leaf and followed by ascorbic acid and BHT, showing that the extracts from dried leaf possess the strongest antioxidant activity. Compared with fresh tissues, SOD activity, ATX activity and CAT activity were high level in the dried tissue. These results suggest that the Momordica charantia L. would be a promising antioxidant source as an alternative antioxidant, based on natural plant resources.
Plant Regeneration via Organogenesis from Leaf Explant Culture of Taraxacum coreanum Nakai
Lee, Hyun-Hwa ; Kim, Young-Soon ; Park, Hyeon-Yong ;
Korean Journal of Medicinal Crop Science, volume 15, issue 1, 2007, Pages 62~66
Plant Regeneration via organogenesis from leaf disk of Korean dandelion was investigated. Leaf disk cultured on MS medium with various combinations of BA (0-4 mg/L) and 2,4-D (0-1 mg/L). Shoot regeneration from leaf explant was observed after 3 weeks of culture. The highest shoot regeneration frequency from leaf disk was obtained with 2 mg/L BA. To analyze the effect of leaf age along shoot formation, we measured number of shoots per explant, shooting rate, fresh and dry weight of leaf explant. The highest number of shoots (11.5) per explant were obtained leaf from 7 weeks old plantlets after seed germination. The regenerated shoots were transferred in 1/2 MS medium with 0.5 mg/L NAA for root formation. Regeneratied plantlets thought organogenesis were growing to whole plants in the pots with acclimation.