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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Journal of Medicinal Crop Science
Journal Basic Information
Journal DOI :
The Korean Society of Medicinal Crop Science
Editor in Chief :
Volume & Issues
Volume 15, Issue 6 - Dec 2007
Volume 15, Issue 5 - Oct 2007
Volume 15, Issue 4 - Aug 2007
Volume 15, Issue 3 - Jun 2007
Volume 15, Issue 2 - Apr 2007
Volume 15, Issue 1 - Feb 2007
Selecting the target year
Inhibitory Effects of Rehmannia glutinosa Liboschitz on Endothelial Cell Proliferation
Lee, Sung-Jin ; Lee, Hak-Kyo ;
Korean Journal of Medicinal Crop Science, volume 15, issue 5, 2007, Pages 311~314
Vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) are the most important angiogenic molecules associated with tumor-induced neovascularization. This study was carried out to investigate inhibitory effect of extracts from root of Rehmannia glutinosa LIBOSCHITZ (Rehmannia Radix and Rehmannia Radix Preparata) on endothelial cell proliferation. The methanol extracts from the medicinal herb were fractionated into n-hexane, ethyl acetate, n-butanol and aqueous fractions. Among the four fractions, the n-butanol fraction from R. Radix on exhibited highly effective inhibition (
inhibition) on the binding of KDR/Flk-1-Fc to immobilized
and then ethyl acetate fraction from R. Radix (
inhibition) at the concentration of
. The n-butanol fraction efficiently blocked the VEGF- and bFGF-induced HUVEC proliferation in a dose-dependent manner, but did not affect the growth of HT1080 human fibrosarcoma cells. The n-butanol fraction more efficiently blocked the binding of KDR/Flk-1-Fc to immobilized
and VEGF- and bFGF-induced human umbilical vein endothelial cell proliferation than the fraction from R. Radix Preparata. Our results suggest that Rehmannia Radix may be used as a candidate for developing anti-angiogenic agent.
Rapid Propagation through Tissue Culture of Cudrania tricuspidata, Medicinal Plant
Lee, Cheul-Ho ; Min, Ji-Yun ; Jung, Ha-Na ; Kim, Kyu-Sick ; Choi, Myung-Suk ;
Korean Journal of Medicinal Crop Science, volume 15, issue 5, 2007, Pages 315~318
An effective rapid propagation method was established through in vitro cultures of the medicinal plant, Cudrania tricuspidata. In vitro plantlets were obtained from in vitro germinated seeds. The various levels of cytokinins (BAP, Kinetin and TDZ) were tested on multiple shoot formation from plantlets. BAP (1.0 mg/l) treatment induced highest number of multiple shoots. Single shoot cultures gave higher initial shoot numbers than 5 shoots per culture. Among the various culture media, the shoot elongation was optimal on 2 MS basal medium without growth regulators. The IAA (2.0 mg/l) treatment induced highest number of roots. IBA (2.0 mg/l) treatment more promoted in vitro root growth than other concentrations. Rooted shoots were transferred directly to small pots with an artificial soil and successfully acclimatized.
Far Infrared Ray Irradiation Stimulates Antioxidant Activity in Vitis flexuosa THUNB. Berries
Eom, Seok-Hyun ; Jin, Cheng-Wu ; Park, Hyoung-Jae ; Kim, Eun-Hye ; Chung, Ill-Min ; Kim, Myong-Jo ; Yu, Chang-Yeon ; Cho, Dong-Ha ;
Korean Journal of Medicinal Crop Science, volume 15, issue 5, 2007, Pages 319~323
Wild grapes have been used as traditional medicinal use and alcoholic beverage production in Korea. The objective of this study is to improve antioxidant properties in Sae-muru by far infrared ray (FIR) treatment, with expecting potential benefits of FIR treatment for wild grape products during manufacturing processes. FIR treatment in berries induced increased content of catechin, epicatechin gallate, epigallocatechin gallate, gallic acid, rutin, ellagic acid, and resveratrol, while content of epicatechin and epigallocatechin was decreased. Although FIR treatment resulted either increased or decreased chemical component groups, presenting in HPLC chromatograms, antioxidant activity in Sae-muru extract was significantly increased by the FIR treatment. Our results suggest that FIR treatment should be an efficient process in the production of high content of bioactive molecules in Sae-muru.
In-vitro Anti-inflammatory Activity of Rubus coreanus Miq. on Nitric Oxide,
, Cycloxygenase-2, and Tumor Necrosis
Production in the Macrophage like Cell Line RAW 264.7 Activated by Lipopolysccharide
Choi, Se-Young ; Lee, Kyou-Chae ; Jeoung, Young-Jun ; Lim, Beong-Ou ;
Korean Journal of Medicinal Crop Science, volume 15, issue 5, 2007, Pages 324~328
To search for immunoactive natural products exerting anti-inflammatory activity, we have evaluated the effects of the ethanol extracts of Rubus coreanus Miq. (ERC) on lipopolysaccharide-induced nitric oxide (NO), tumor necrosis
production by RAW 264.7 macrophage cell line. Our data indicate that this extract is a potent inhibitor of NO production and it also significantly decreased
production. Consistent with these results, the protein level of inducible Nitric Oxide Synthase (iNOS) and cyclooxygenase-2 (COX-2) was inhibited by ethanol extracts of ERC in a dose-dependent manner. These results suggest that ERC may exert anti-inflammatory and analgesic effects possibly by suppressing the inducible NO synthase and COX-2 expressions.
Antioxidant and Anticancer Activity of Fractions from Picrasma quassioides (D. Don) Benn. Methanolic Extract
Yin, Yu ; Wang, Myeong-Hyeon ;
Korean Journal of Medicinal Crop Science, volume 15, issue 5, 2007, Pages 329~334
The potential antioxidant and anticancer activities of Hexane, EtOAc (Ethyl acetate), BuOH (n-Buthanol) and water fractions from methanolic (MeOH) extract of Picrasma quassioides (D. Don) Benn. were evaluated in vitro. Tested fractions showed strong antioxidant activity, especially EtOAc fraction had the highest activity (
), containing high total phenolics and total flavonoids contents, showed
respectively. Anticancer activity of these fractions was tested by MTT assay on HT-29 (the human colon carcinoma cells) cell line. BuOH fraction not only showed very high anticancer activity, but also had no cytotoxic effect on 293 (the human normal kidney cells) cell line. Considering these results, we used BuOH fraction of MeOH crude extract from P. quassioides (D.Don) Benn. to do assessment of apoptosis by flow cytometry and the mRNA expression levels of widely established apoptotic-related genes on HT-29 cell line. All the experiments showed that BuOH fraction can induce apoptosis on HT-29 cell line strongly. Taken together, methanolic extract of P. quassioides has potential for antioxidant and anticancer activities products.
Antioxidant Potential in the Fruits of Pyrus Species (Pear) in Korea
Park, Young-Ki ; Kim, Sea-Hyun ; Chung, Hun-Gwan ;
Korean Journal of Medicinal Crop Science, volume 15, issue 5, 2007, Pages 335~338
Five samples (Bakwoonbae, Chungsilbae, Sandolbae, Dolbae, and Chuwhangbae) of Pyrus species differing in their origin of production were analyzed for total phenolic content, vitamin C content, and antioxidant activities. Two antioxidant activities were measured by the free-radical scavenging activity and reducing power method. Total phenolic content of the Chungsilbae extract (20.35 mg/g) was higher than those of other pears. Vitamin C content in five Pyrus species ranged from
in Sandolbae to
in Bakwoonbae. Chungsilbae also had the highest free radical scavenging activity (76.56%) and reducing power (0.35).
Field Performance and Morphological Characterization of Transgenic Codonopsis lanceolata Expressing
Ghimire, Bimal Kumar ; Li, Cheng Hao ; Kil, Hyun-Young ; Kim, Na-Young ; Lim, Jung-Dae ; Kim, Jae-Kwang ; Kim, Myong-Jo ; Chung, Ill-Min ; Lee, Sun-Joo ; Eom, Seok-Hyun ; Cho, Dong-Ha ; Yu, Chang-Yeon ;
Korean Journal of Medicinal Crop Science, volume 15, issue 5, 2007, Pages 339~345
Field performance and morphological characterization was conducted on seven transgenic lines of Codonopsis lanceolata expressing
gene. The shoots were obtained from leaf explants after co-cultivation with Agrobacterium tume-faciens strain LBA 4404 harboring a binary vector pYBI 121 that carried genes encoding
methyltransferase gene (
) and a neomycin phosphotransferase II gene (npt II) for kanamycin resistance. The transgenic plants were transferred to a green house for acclimation. Integration of T-DNA into the
generation of transgenic Codonopsis lanceolata genome was confirmed by the polymerase chain reaction and southern blot analysis. The progenies of transgenic plants showed phenotypic differences within the different lines and with relative to control plants. When grown in field, the transgenic plants in general exhibited increased fertility, significant improvement in the shoot weight, root weight, shoot height and rachis length with relation to the control plants. However, all seven independently derived transgenic lines produced normal flower with respect to its shape, size, color and seeds number at its maturity. Indicating that the addition of a selectable marker gene in the plant genome does not effect on seed germination and agronomic performance of transgenic Codonopsis lanceolata.
progenies of these plants were obtained and evaluated together with control plant in a field experiment. Overall, the agronomic performance of
progenies of transgenic Codonopsis lanceolata showed superior to that of the seed derived non-transgenic plant. In this study, we report on the morphological variation and agronomic performance of transgenic Codonopsis lanceolata developed by Agrobacterium transformation.
Somatic Embryogenesis and Plant Regeneration from Embryogenic cell Suspension Cultures of Schisandra chinensis Baill
Li, Cheng Hao ; Niu, YudA ; Zhao, Bo ; Ghimire, Bimal Kumar ; Kil, Hyun-Young ; Heo, Kwon ; Kim, Myong-Jo ; Eom, Seok-Hyun ; Cho, Dong-Ha ; Yu, Chang-Yeon ;
Korean Journal of Medicinal Crop Science, volume 15, issue 5, 2007, Pages 346~351
An efficient somatic embryogenesis and plant regeneration protocol was developed for Schisandra chinensis Baill, using embryogenic cell suspensions and optimized media conditions. Friable embryogenic callus was induced from cotyledonary leaf and hypocotyl explants of 7 days old seedlings on MS agar medium supplemented with 1.0 to
of 2,4-dichlorophenoxyacetic acid (2,4-D). Fast growing and well dispersed embryogenic cell suspensions were developed within two months when embryogenic calli were transferred to MS liquid medium containing
. One third strength of MS medium was the best for both overall growth and development of somatic embryos in liquid culture. Over 3400 viable somatic embryos were produced from each 150 ml flask with an initial cell density of 30 mg in 30 ml medium. Germinated somatic embryos developed in liquid medium converted into plantlets after transferred to half-strength MS semi-solid medium. Approximately 90% of the converted plantlets were successfully transplanted to soil and grew into fertile plants.
The Influence of Long-term Aloe Supplement on Anti-oxidative Defenses and Cholesterol Content in Brain and Kidney of Aged Rats
Lim, Beong-Ou ; Park, Pyo-Jam ; Park, Dong-Ki ; Choi, Wahn-Soo ; Kim, Jong-Dai ; Yu, Byung-Pal ;
Korean Journal of Medicinal Crop Science, volume 15, issue 5, 2007, Pages 352~356
The present study was investigated the anti-oxidative effects of aloe vera ingestion on brain and kidney in aged rats by monitoring several oxidative-related parameters. Male specific pathogen-free Fischer 344 rats were randomly divided into four groups of five rat each: Group A was fed test chow without aloe supplementation; Group B was fed a diet containing a 1% freeze-dried aloe filet; Group C was fed a diet containing a 1% charcoal-processed, freeze-dried aloe filet; and Group D was fed a diet containing a charcoal-processed, freeze-dried, whole leaf aloe in drinking water. Analyses of tissues were done at 4 months and 16 months of age. Results showed that a long-term intake of aloe, regardless of the preparation used, enhanced antioxidant defenses against lipid peroxidation, as indicated by reduced phosphatidylcholine hydroperoxide levels in both brain and kidney. The additional benefit of aloe intake on the anti-oxidative action was evidenced by enhanced superoxide dismutase and catalase activity in all aloe-ingested groups. Another beneficial effect of aloe shown in this study, although not an anti-oxidative parameter, was its cholesterol-lowering effect as detected in brain and kidney with significant decreases at age16 months of aloe-fed rats. Based on these findings, we conclude that a long-term dietary aloe supplementation modulated the anti-oxidative defense systems and cholesterol level.
Vegetation Structure of Peucedanum japonicum Thunb. Community in Southern Coast of Korea
Kim, Seong-Min ; Shin, Dong-Il ; Yoon, Seong-Tak ; Song, Hong-Seon ;
Korean Journal of Medicinal Crop Science, volume 15, issue 5, 2007, Pages 357~361
This study was conducted to evaluate the vegetation structure of the Peucedanum japonicum community by the phytosociological method of floristic composition table and cluster analysis on the southern coast of Korea. The vegetation of the Peucedanum japonicum community was classified mainly into 2 communities such as the Miscanthus sinensis community and the Lysimachia mauritiana-Rosa wichuraiana community. The Carex boottiana and Sedum oryzifolium community were classified as the lower rank of Miscanthus sinensis community. On level 1 of the cluster analysis of plant species, they were classified into Lysimachia mauritiana and Rosa wichuraiana group, also Miscanthus sinensis, Carex boottiana and Sedum oryzifolium in Peucedanum japonicum community, which is similar to the community classification shown in the synoptic table. On level 1 of the cluster analysis of relev, inland coast with Jejudo was Lysimachia mauritiana and Rosa wichuraiana of group such as level 1 of the cluster analysis of plant species, and island coast with Geomundo was Miscanthus sinensis Carex boottiana and Sedum oryzifolium of group such as cluster analysis of plant species.
Distribution and Habitat Chracteristics of Lonicera japonica Thunb. in the Inland and the Seashore Areas of Korea
Kim, Seong-Min ; Shin, Dong-Il ; Yoon, Seong-Tak ; Song, Hong-Seon ;
Korean Journal of Medicinal Crop Science, volume 15, issue 5, 2007, Pages 362~366
This study was conducted to evaluate the status of habitat distribution, environmental characteristics and plant species growing with Lonicera japonica in its habitats. In the distribution of plant coverage below 30%, it was 84.6% in the inland area and 80.7% in the seashore area. There was similar coverage in both inland and seashore areas. But the average relative coverage in the seashore area was 26.1%, which is a little higher than that of the inland area (22.5%). In the habitats, both inland and seashore areas showed a high distribution ratio below 10 degrees. But among them, inland areas showed a high distribution ratio (62.4%) below 10 degrees, which was about twice as much as the seashore areas (32.2%). Habitat distribution was most often found on the southern slopes in both inland and seashore areas. The average soil pH of Lonicera japonica habitat was 5.2 in the inland areas and 6.9 in the seashore areas. Also, the seashore areas showed little content of
, but high content of K, Mg, Na on the other hand compared to the inland area. Miscanthus sinensis was the highest importance value (16.4) among plants growing with Lonicera japonica in their habitats in both inland and seashore areas. But in the inland areas, Artemisia princeps showed the highest importance value, and Miscanthus sinensis was the highest in the seashore areas.