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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Medicinal Crop Science
Journal Basic Information
Journal DOI :
The Korean Society of Medicinal Crop Science
Editor in Chief :
Volume & Issues
Volume 16, Issue 6 - Dec 2008
Volume 16, Issue 5 - Oct 2008
Volume 16, Issue 4 - Aug 2008
Volume 16, Issue 3 - Jun 2008
Volume 16, Issue 2 - Apr 2008
Volume 16, Issue 1 - Feb 2008
Selecting the target year
Immune Activities of Rhodiola sachalinensis A. Bor Extracts Isolated with Various Extraction Process
Kim, Cheol-Hee ; Kwon, Min-Chul ; Han, Jae-Gun ; Ha, Ji-Hye ; Jeong, Hyang-Suk ; Choi, Geun-Pyo ; Park, Uk-Yeon ; Nam, Jong-Hyun ; Hwang, Baik ; Lee, Hyeon-Yong ;
Korean Journal of Medicinal Crop Science, volume 16, issue 6, 2008, Pages 383~389
This study was performed to compare effect of immune activities of Rhodiola sachalinensis by various extraction process with different temperature and extraction solvents. Experiments were performed for investigate the immune activities on human B and T cell growth and secretion of their cytokines. Also, antibodies in serum were investigated in female ICR mouse by feeding the extracts of R. sachalinensis at doses of 40, 120 and 360 mg/kg orally for 15 days. The immune cell growth and secretion of cytokines (IL-6, TNF-
) on human B and T cells were increased by adding R. sachalinensis extracts compare to the control. Also, total serum IgG levels increased by feeding R. sachalinensis extracts. It can be conclude that optimum condition for efficient extraction of R. sachalinensis as functional material is slovent extraction process using water with ultrasonification at below
than typical process.
Residues of Tolclofos-methyl, Azoxystrobin and Difenoconazole in Ginseng Sprayed by Safe Use Guideline
Kim, Jang-Eok ; Kim, Tae-Hwa ; Kim, Young-Hwan ; Lee, Ju-Hee ; Kim, Jin-Su ; Paek, Seung-Kyoung ; Choi, Su-Yeon ; Youn, Young-Nam ; Yu, Yong-Man ;
Korean Journal of Medicinal Crop Science, volume 16, issue 6, 2008, Pages 390~396
The aim of this work is to assess the safety of fungicide tolclofos-methyl, difenoconazole and azoxystrobin in ginseng sprayed by safe use guideline. When tolclofos-methyl was sprayed on ginseng by safe use guideline, the residue amounts (MRL) of it in ginseng was 0.13 mg/kg which is below than 0.3 mg/kg, maximum residue limit established by Korea Food & Drug Administration (KFDA). The residue amounts of ginseng parts, head part was 0.37 mg/kg and main body part was 0.13 mg/kg. In case of difenoconazole, the residue amounts in ginseng was 0.81 mg/kg. which was exceed the MRL, 0.2 mg/kg. By the analyze results of ginseng part, the residues of head and main body part were 3.01 and 0.40 mg/kg, respectively. In experiment of vinyl mulching, the residue amount of difenoconazole in ginseng was 0.05 mg/kg. The residue amounts of azoxystrobin in ginseng sprayed by safe use guideline was 0.14 mg/kg. This residue was not exceed the MRL 0.5 mg/kg. The residue amounts by ginseng parts was 0.51 mg/kg for head part and 0.28 mg/kg for main body part. In case of vinyl mulching, the residue amount of azoxystrobin was 0.02 mg/kg.
Changes in Total Polyphenol Contents and DPPH Radical Scavenging Activity of Agrimonia pilosa According to Harvest Time and Various Part
Jang, Sang-Hun ; Yu, Eun-Ae ; Han, Ki-Soo ; Shin, Sung-Chul ; Kim, Hee-Kyu ; Lee, Sang-Gyeong ;
Korean Journal of Medicinal Crop Science, volume 16, issue 6, 2008, Pages 397~401
Changes in the contents of total phenolic compounds in as Agrimonia pilosa well as their antioxidant capacity according to the havest time and positions were examined. The contents of the total phenolic compounds were determined by extraction with MeOH. Among havest times harvestry in July showed highest contents of the total phenolic compounds and harvestry in May showed lowest contents of the phenolic compounds. Among the 4 positions (root, branch, leaf, flower) of Agrimonia pilosa the root contained highest contents of the phenolic compounds. The antioxidant capacities of Agrimonia pilosa were increased roughly with increasing level of contents of phenolic compounds according to positions.
Enhancement of Immune Activities of Natural Water-Soluble Sulforaphane by Nano Encapsulation Process
Ha, Ji-Hye ; Han, Jae-Gun ; Jeong, Hyang-Suk ; Oh, Sung-Ho ; Kwon, Min-Chul ; Choi, Young-Beom ; Ko, Jung-Rim ; Lee, Hyeon-Yong ;
Korean Journal of Medicinal Crop Science, volume 16, issue 6, 2008, Pages 402~408
This study was performed to investigate improving immune activities of natural water-soluble sulforaphane extracted from Brassica oleracea var. italica by nano encapsulation process. The nanoparticles of the sulforaphane extracted with ultrasonification process at
promoted human B and T cell growth, about
compared to the control. The secretion of IL-6 and TNF-
from T cells were also enhanced as
, respectively, by the adding nano samples. NK cell activation was improved about 8%, compare to the control in adding cultured medium of T cell added nano samples. It was also found that sulforaphane extracted from B. oleracea var. italica had highly inhibitory activity on hyaluronidase as
. It can be concluded that natural water-soluble sulforaphane samples by nano-encapsulation, each size is 200 nm, extracted from B. oleracea var. italica has high immune activities through higher efficiency of bio-activation than conventional extracts.
Moutan Cortex Extract Inhibits Amyloid
Protein (25-35)-induced Neurotoxicity in Cultured Rat Cortical Neurons
Kim, Joo-Youn ; Ju, Hyun-Soo ; Ban, Ju-Yeon ; Song, Kyung-Sik ; Seong, Yeon-Hee ;
Korean Journal of Medicinal Crop Science, volume 16, issue 6, 2008, Pages 409~415
Moutan cortex, the root bark of Paeonia suffruticosa Andrews (Paeoniaceae), has pharmacological effects such as anti-inflammatory, antiallergic, analgesic and antioxidant activities. We investigated a methanol extract of Moutan cortex for neuroprotective effects on neurotoxicity induced by amyloid
) (25-35) in cultured rat cortical neurons. Exposure of cultured cortical neurons to
(25-35) for 24 h induced neuronal apoptotic death. Moutan cortex inhibited
(25-35)-induced neuronal cell death at 30 and
, which was measured by a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and Hoechst 33342 staining. Moutan cortex inhibited
(25-35)-induced elevation of intracellular calcium concentration (
), and generation of reactive oxygen species (ROS) which were measured by fluorescent dyes. Moutan cortex also inhibited glutamate release into medium induced by
(25-35), which was measured by HPLC. These results suggest that Moutan cortex prevents
(25-35)-induced neuronal cell damage by interfering with the increase of
, and then inhibiting glutamate release and ROS generation. Moutan cortex may have a therapeutic role in preventing the progression of Alzheimer's disease.
Changes of Chlorophyll Fluorescence and Photosynthesis under Different Shade Materials in Korean Ginseng(Panax ginseng C. A. Meyer)
Won, Jun-Yeon ; Lee, Chung-Yeol ; Oh, Dong-Joo ; Kim, Sung-Man ;
Korean Journal of Medicinal Crop Science, volume 16, issue 6, 2008, Pages 416~420
This study was conducted to investigate the influence of shading material on the chlorophyll fluorescence, photosynthesis, transpiration, stomatal conductance and its any correlations in Panax ginseng C.A.Meyer. Fo was higher in polyethylene shade net than in silver-coated shading plate, but this treatment caused a lower Fm in comparison with silver-coated shading plate. Also, Fv/Fm and PhiPS2 showed higher in silver-coated shading plate than in polyethylene shade net. The relationship between net photosynthetic rate and transpiration, stomatal conductance were increased as the PAR (Photosynthetic active radiation) was increased and reached maximum at the
of PAR in all of leaves, and the higher in silver-coated shading plate than in polyethylene shade net. A linear equation was obtained between net photosynthetic rate and transpiration, net photosynthetic rate and stomatal conductance. SPAD was higher in silver-coated shading plate than in polyethylene shade net.
Influence of Forcing Cultivation Time on Cut Flower, Root Quality, and Yield in Peony (Paeonia lactiflora Pall. cv. Taebaek)
Bae, Su-Gon ; Kim, Jwoo-Hwan ; Park, Sang-Jo ; Kim, Jae-Cheol ;
Korean Journal of Medicinal Crop Science, volume 16, issue 6, 2008, Pages 421~426
Three year-old peony (Paeonia. lactiflora Pall. cv. Taebaek) was cultivated in green-house at Jan. 15, Feb. 15, or Mar. 15, respectively. The mean of temperature during the forcing cultivation was higher (air;
) than that of open-field condition. From sprouting to flowering in peony cultivated at Jan. 15 was about 54 days, which is shorted the cut flower periods (ca. 26 days) compared with the open-field cultivation. However, earlier forcing cultivars were very susceptible to pathogens such as powdery mildew or gray mold. The yield in green house was also lower than in the open-field cultivation. The content of bioactive compounds such as paeoniflorin and albiflorin in green-house cultivars was similar that of open-field cultivars. These results showed the forcing cultivation time of peony at Feb. 15 in green-house was most desirable for commercialization.
Comparison of Antioxidant and Glutathione S-Transferase Activities of Extracts from Acer mono and A. okamotoanum
Jin, Ling ; Han, Jae-Gun ; Ha, Ji-Hye ; Jeong, Hyang-Suk ; Kwon, Min-Chul ; Jeong, Myoung-Hoon ; Lee, Hak-Ju ; Kang, Ha-Young ; Choi, Don-Ha ; Lee, Hyeon-Yong ;
Korean Journal of Medicinal Crop Science, volume 16, issue 6, 2008, Pages 427~433
This study was performed to investigate antioxidant activities and glutathione S-transferase (GST) activity according to parts of the Acer mono and A. okamotoanum. Most extracts showed high scavenging activities on DPPH. Especially, the bark of A. okamotoanum showed higher activity as 98.4% than the control, BHA as 96.5%. A. mono and A. okamotoanum showed high ability on nitrite scavenging, but decreasing tendency according to decreasing of pH. On SOD-like test, the wood of A. okamotoanum showed highest activity as 35.4% at 1.0mg/ml concentration. Also, the extracts obtained high activity on GST test. Therefore, the water extracts from the bark of A. mono and A. okamotoanum have relatively good antioxidant activity and GST activity. Especially, the bark of A. okamotoanum showed the highest activity on all of extracts, could be the use of functional foods and biomaterials.
Effect of Variety and Shading Material on Growth Characteristics and Ginsenoside Contents of 2-Year-Old Ginseng (Panax ginseng C. A. Meyer) Grown in Imperfectly Drained Paddy Soil
Lee, Sung-Woo ; Yeon, Byeong-Yeol ; Kim, Chung-Guk ; Shin, Yoo-Su ; Hyun, Dong-Yun ; Kang, Seung-Won ; Cha, Seon-Woo ;
Korean Journal of Medicinal Crop Science, volume 16, issue 6, 2008, Pages 434~438
To selection of optimal shading material, two-year-old ginseng (Panax ginseng C. A. Meyer) of new cultivar, 'Cheonpoong' (CP), and native species 'Hwangsookjong' (HS) were cultured under three kinds of shading materials such as three-layered blue and a one-layered black PE (polyethylene) net (TBSB), blue PE sheet (BS), and aluminium coated PE sheet (AS) in imperfectly drained paddy soil. Growth characteristics, yield and ginsenoside contents were investigated under three shading materials. Yield and ginsenoside contents of ginseng were distinctly affected by intensity and quality of sunlight penetrated through shading materials. Light transmission ratio, air and soil temperature according to shading materials were higher in order of BS, AS, and TBSB. However, ratio of aerial phase and porosity of the soil were higher in order of AS, BS, and TBSB, respectively. There was no significantly difference in the ratio of rusty colored root by shading materials. CP showed higher stem length, leaf area, and root weight than that of HS, while the former showed distinctly lower discolored leaf ratio than that the other. Eight kinds of ginsenosides content of CP were higher than that of HS in
, Re, Rf,
and Rc except
. Total ginsenoside contents of CP was distinctly higher than that of HS. Total ginsenoside contents as affected by shading materials was higher in order of BS, TBSB, and AS in CP, while TBSB, BS, and AS in HS.
Effect of Immune Activity on Berberis koreana Palibin by Ultra High Pressure Low Temperature Process
Jin, Ling ; Han, Jae-Gun ; Ha, Ji-Hye ; Jeong, Hyang-Suk ; Kwon, Min-Chul ; Ahn, Ju-Hee ; Kim, Jin-Chul ; Choi, Geun-Pyo ; Chung, Eul-Kwon ; Lee, Hyeon-Yong ;
Korean Journal of Medicinal Crop Science, volume 16, issue 6, 2008, Pages 439~445
This study was performed to investigate a methodology of improving immune activities of Berberis koreana Palibin by ultra high pressure on low temperature extraction process. Extraction yield was enhanced up to more than 35% by above process, compare to the control and extraction average. The cytotoxicity on human kidney cell (HEK293) was showed below 20.4%, 21.6% in adding 1.0 mg/ml of the highest concentration. Generally, the extracts by ultra high pressure extraction process showed less toxicity about 5% than the other processes. It could tell that toxic materials that existing in the plant could be reduced or broken by ultra high pressure process due to can be broken bond such the hydrogen bond, the electrostatic bond, the van der Waals bond, and the hydrophobic bond, can be broken by high pressure. The result could be employed to develop a new type of functional food from B. koreana Palibin by low temperature high pressure process.
Extraction and Preprocessing Methods for Ginsenosides Analysis of Panax ginseng C.A. Mayer
Kim, Geum-Soog ; Hyun, Dong-Yun ; Kim, Young-Ock ; Lee, Sung-Woo ; Kim, Young-Chang ; Lee, Seung-Eun ; Son, Yeong-Deck ; Lee, Min-Jeong ; Park, Chung-Berm ; Park, Ho-Ki ; Cha, Seon-Woo ; Song, Kyung-Sik ;
Korean Journal of Medicinal Crop Science, volume 16, issue 6, 2008, Pages 446~454
An advanced extraction method by ultrasonic extraction with applied solid phase extraction (SPE) has been developed for the determination of simultaneous eight major ginsenosides, namely ginsenosides Rg1, Re, Rf, Rb1, Rg2, Rc, Rb2, and Rd in the root of Panax ginseng. Four extraction methods including n-BuOH reflux extraction (Method A), 70% EtOH reflux extraction (Method B), 50% MeOH reflux extraction with SPE (Method C), and 50% MeOH ultrasonication with SPE clean-up process (Method D) were investigated for the determination of eight major ginsenosides. Total contents of ginsenosides were highest by extraction of Method C as
. However, Method D was evaluated as relatively simpler and more efficient method due to short extraction time, small solvent consumption and less expensive, compared to conservative reflux method. Ginsenosides were also satisfactorily separated with good resolution and the accuracy range was between 1.05 and 4.06% as relative standard deviation (RSD) by Method D. SPE condition and HPLC condition were further optimized for determination of eight major ginsenosides by the ultrasonic extraction method. Conclusively, ultrasonic extraction of 2 g sample of ginseng using ultrasonic bath and 1 loading for SPE was evaluated as proper condition for extraction of ginseng.