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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Journal of Medicinal Crop Science
Journal Basic Information
Journal DOI :
The Korean Society of Medicinal Crop Science
Editor in Chief :
Volume & Issues
Volume 17, Issue 6 - Dec 2009
Volume 17, Issue 5 - Oct 2009
Volume 17, Issue 4 - Aug 2009
Volume 17, Issue 3 - Jun 2009
Volume 17, Issue 2 - Apr 2009
Volume 17, Issue 1 - Feb 2009
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Antioxidative and Hepatoprotective Effects of Lythrum salicaria
Lee, Seung-Eun ; Park, Chun-Geun ; Ahn, Young-Sup ; Son, Yeong-Deck ; Cha, Sun-Woo ; Seong, Nak-Sul ;
Korean Journal of Medicinal Crop Science, volume 17, issue 1, 2009, Pages 1~7
Several parts of Lythrum salicaria were used for this study. Scavenging activities on radicals, inhibitory activity on linoleic acid peroxidation and total phenol contents of extracts from root, flower, and aerial part were evaluated. Flower and root selected from in vitro assay were subjected to in vivo assay on
-induced liver injury rat model for two weeks. Carbon tetrachloride intoxication on rats produced large amounts of hepatic lipid peroxidation product, thiobarbituric acid reactive substance (TBARS) compared with normal rats. Treatment with root extract of L. salicaria (LSR) showed effective inhibitory activity on lipid peroxidation product. Administration with LSR extract significantly alleviated
-induced increase in GPT activity which were more effective than silymarin. The results of this study suggest that root and flower of L. salicaria have antioxidant and liver protecting activities, and root part is the most effective candidate to develop a new functional material.
Immunomodulatory Effect of Pueraria lobata on the Functional Activation of Macrophages by Lipopolysaccharide Treatment
Shen, Ting ; Kim, Jong-Dai ; Cho, Jae-Youl ;
Korean Journal of Medicinal Crop Science, volume 17, issue 1, 2009, Pages 8~14
Pueraria lobata (Willd.) Ohwi was investigated to check its modulatory effects on the activation of macrophages upon inflammatory conditions treatment. For this purpose, we examined several inflammatory responses such as nitric oxide (NO) production, reactive oxygen species (ROS) generation, cytoprotection and phagocytosis under the treatment of methanol extract from P. lobata (Pl-ME). Pl-ME dose-dependently blocked NO production in lipopolysaccharide (LPS)- stimulated RAW264.7 cells but not sodium prusside (SNP)-generated NO release. The NO inhibition seemed to be due to blocking inducible NO synthase (iNOS), since Pl-ME suppressed its expression in a NF-
-independent manner. Similarly, this extract also effectively protected RAW264.7 cells from LPS-induced cytotoxicity. However, Pl-ME did not block ROS generation and rather it enhanced. Finally, this extract negatively modulated FITC-dextran uptake. Therefore, our data suggested that Pl-ME may be involved in negatively regulating some macrophage-mediated inflammatory responses such as NO production and phagocytic uptake.
Different of Total Phenolics and Flavonoids, Radical Scavenging Activities and Nitrite Scavenging Effects of Momordica charantia L. According to Cultivars
Boo, Hee-Ock ; Lee, Hyun-Hwa ; Lee, Jang-Won ; Hwang, Sung-Jin ; Park, Sang-Un ;
Korean Journal of Medicinal Crop Science, volume 17, issue 1, 2009, Pages 15~20
This study was carried out in order to determine the biological activities such as antioxidant activities in nine cultivars of Momordica charantia L. (Korea, China, Japan and Philippine native cultivars). Their antioxidant activities were measured using DPPH free radical scavenging, ABTS cation radical scavenging, and Nitrite scavenging ability. The highest total polyphenol content (16.82
) measured in the native Korea cultivar, and this value was 4.0
higher than that of the 'Verde Beuhas' cultivar (12.82
). The 'Peacock' cultivar had the highest total flavonoid amount which was 4.38
. The free radical scavenging activity using DPPH method was the highest in the native China cultivar (
). ABTS cation radical scavenging activity according to cultivars was significantly higher in 'Peacock'. Nitrite scavenging ability showed the most remarkable effect at the pH 1.2, exhibited to 81.5
86.9% by addition of ethanol extract 1
from Momordica charantia L. These results suggest that Momordica charantia L. had the potent biological activities, and that their activities exhibited differently depending on cultivars.
Antioxdant Activity of Hibiscus cannabinus L. Leaves in Different Growth Time
Jin, Cheng-Wu ; Eom, Seok-Hyun ; Park, Hyoung-Jae ; Ghimeray, Amal Kumar ; Yu, Chang-Yeon ; Cho, Dong-Ha ;
Korean Journal of Medicinal Crop Science, volume 17, issue 1, 2009, Pages 21~25
The results on the useful functional compounds of kenaf (Hibiscus cannabinus L.) leaves cultivated in reclaimed lands and the biological activity effects of extracts were as follows. On 98 days after sowing (DAS) Tainung-2 showed the highest total chlorophyll content (1.68 mg/g), and on 141 DAS Dowling showed higher chlorophyll content (1.50 mg/g) than the other two did. In all cultivars the total chlorophyll content was higher on 141 DAS than on 98 DAS. Total polyphenol and total flavonoid contents were the highest in Tainung-2 (30.50 mg/g and 57.03 mg/g, respectively), and total polyphenol and total flavonoid contents (30.50 mg/g and 57.03 mg/g, respectively) were the highest in 30% ethanol extraction. Ascorbic acid contents were higher on 141 DAS than on 98 DAS in three cultivars. SOD activities of kenaf leaf extract were generally over 90%. DPPH radical scavenging activity of Tainung-2 was higher than others.
DNA Sequencing and Phylogenetic Analysis of the 18S rRNA Gene of Atractylodes japonica Koidz and Analysis of Atractylon
Bae, Young-Min ;
Korean Journal of Medicinal Crop Science, volume 17, issue 1, 2009, Pages 26~32
The region containing 18S rRNA gene, ITS 1 and part of the 5.8S rRNA gene of the Atractylodes japonica Koidz was amplified by PCR and the product cloned in a pBluescript SK II plasmid. DNA sequence of the cloned DNA was determined and submitted to the GenBank (accession number EU678363). Phylogenetic analysis of the ITS 1 DNA showed close similarity with the other plant species of the family Compositae. The extract of the plant materials of five different members of the family Compositae was analyzed by HPLC to detect atractylon. Extract of the A. japonica Koidz showed presence of significant amount of atractylon. However, noticeable amount of atractylon was not detected by the same analyses from the extracts of the other plants belonging to the family Compositae including Artemisia capillaris, Chrysantemum zawadskii, Eclipta prostrata or Taraxacum platycarpum.
Molecular Characterization of the Perilla frutescens Limonene Gene (PFLS) by Agroinfiltration into Nicotiana benthamiana
Seong, Eun-Soo ; Seo, Eun-Won ; Kim, Hyoung-Seok ; Heo, Kweon ; Lee, Ju-Kyung ; Chung, Ill-Min ; Ghimire, Bimal Kumar ; Kim, Myong-Jo ; Lim, Jung-Dae ; Yu, Chang-Yeon ;
Korean Journal of Medicinal Crop Science, volume 17, issue 1, 2009, Pages 33~38
The full-length cDNA encoding Perilla frutescens limonene synthase (PFLS) (603 amino acids, GenBank accession no. D49368) was cloned. To elucidate the role of PFLS in gene regulation, we transiently transformed full-length PFLS into tobacco plants. PFLS mRNA was first detected in the intact leaves of the plants at 6 h, and the LS transcript level increased after 12 h in leaves treated with oxidative stress-related chemicals. The transient overexpression of PFLS resulted in increased transcription of NbPR1 and NbSIP in Nicotiana benthamiana leaves. Thus, our result confirmed that the infiltration of PFLS gene act as a transcriptional regulator of NbPR1 or NbSIP genes in the tobacco.
Effects of LED on Growth, Morphogenesis and Eleutheroside Contents of in vitro Cultured Plantlets of Eleutherococcus senticosus Maxim
Jeong, Jae-Hun ; Kim, Young-Seon ; Moon, Heung-Kyu ; Hwang, Sung-Jin ; Choi, Yong-Eui ;
Korean Journal of Medicinal Crop Science, volume 17, issue 1, 2009, Pages 39~45
The effects of red, blue, and far-red light by illumination of light emitting diodes (LEDs) on growth, morphogenesis and eleutheroside contents of in vitro plantlets of Eleutherococcus senticosus were examined. As a control, plantlets were grown under a broad spectrum white fluorescent lamp (16/8 h illumination). The length of plantlets grown under the red/blue LEDs was taller than those under fluorescent lamps. Leaf area, root length and fresh weight of plantlets were highest under blue light compared to other kinds of light sources. Chlorophyll contents in plantlets grown under fluorescent lamps were higher than those in plantlets grown under LED illumination. Production of eleuthroside B and E in plantlets was highest under blue LED. However, production of eleuthroside E1 was highest under fluorescent lamps. These results suggest that plant growth and eleuthroside accumulation can be controlled by wave length of light under LED illumination system.
Phylogenetic Analysis of Schizonepeta Spike on the Basis of DNA Sequences
Jigden, Baigalmaa ; Kim, Myung-Kyum ; Noh, Jong-Hun ; Sun, Hua ; Yang, Deok-Chun ;
Korean Journal of Medicinal Crop Science, volume 17, issue 1, 2009, Pages 46~53
Schizonepeta spike (Korean name "Hyung-Gae") has been used for oriental medicinal purposes in Korea, China and Japan. In this study, twenty six "Hyung-Gae" samples were collected including nine certified Schizonepeta tenuifolia plants, and seventeen commercially marketed "Hyung-Gae" products. Chloroplast trnL-F and rDNA ITS regions of the "Hyung-Gae" samples were sequenced and used to identify whether the samples were genuine S. tenuifolia or not. As the result, the trnL-F and ITS sequences of all the "Hyung-Gae" samples were shown to be identical and it was proven that commercially available medicinal products "Hyung-Gae" are genuine S. tenuifolia. Phylogenetic tree of S. tenuifolia using the trnL-F sequences was constructed and compared with phylogenetic tree using ITS of rDNA region sequences. In these tree, S. tenuifolia was affiliated in the family Lamiaceae. It is proven that trnL-F and ITS phylogenetic trees are useful to study taxonomic position of S. tenuifolia.
Enhancement of Immuno Modulatory Activities of Rubus coreanus Miquel Extracts by Nano-encapsulation Process
Han, Jae-Gun ; Kwon, Min-Chul ; Ha, Ji-Hye ; Jeong, Hyang-Suk ; Kim, Young ; Jeong, Myoung-Hoon ; Kim, Jin-Chul ; Lee, Hyeon-Yong ;
Korean Journal of Medicinal Crop Science, volume 17, issue 1, 2009, Pages 54~60
This study was performed to improve immune activities of Rubus coreanus Miquel by encapsulation of nanoparticles. Immuno-activities of R. coreanus were investigated through aqueous extracts associated with process of water at
. It showed high promotion of human B and T cells growth about 50%, compared to the case of other conditions. The secretion of IL-6 and TNF-
was also enhanced as
pg/cell, results by adding nano samples. NK cell activation was improved up to 29% higher than the conventional extraction process. The secretion of NO from macrophage showed 14.9
on the nano-encapsulation process extracts, which was higher than others. The size of nanoparticles was in the range of 50
300 nm, which can effect the penetration into the cells. It was clearly observed by real time confocal microscope.
Antimicrobial Agent from Schima wallichii ssp. liukiuensis against Candida spp.
Shin, Kuem ; Min, Ji-Yun ; Kang, Seung-Mi ; Park, Dong-Jin ; Song, Hyun-Jin ; Kwon, Oh-Woong ; Yang, Jae-Kyung ; Karigar, Chandrakant S. ; Choi, Myung-Suk ;
Korean Journal of Medicinal Crop Science, volume 17, issue 1, 2009, Pages 61~67
This study carried out development of a natural antimicrobial agent from Schima wallichii ssp. liukiuensis. Compound I exhibiting potent antimicrobial activity against Candida spp. was isolated from the methanol extracts of Schima wallichii ssp. liukiuensis. The structure of I identified as a sterol glycoside consisted of a trisaccharide and
-sitosterol. Trisaccharide composed of L-rhamnose, D-galactose and D-glucose residues. The antimicrobial activity of I was selective on yeast rather than bacteria or other fungi. Compound I was demonstrated to be ineffective against toxicity to mouse liver cells where as protective to human dermal fibroblast cells at low concentrations. Thus, it is reasonable to expect a sterol glycoside (I) as a valuable alternative for synthetic antifungal.
Protective Effect of Vitis amurensis Stems and Leaves Extract on Hydrogen Peroxide-induced Oxidative Neuronal Cell Damage in Cultured Neurons
Kim, Joo-Youn ; Ju, Hyun-Soo ; Ban, Ju-Yeon ; Song, Kyung-Sik ; Bae, Ki-Hwan ; Seong, Yeon-Hee ;
Korean Journal of Medicinal Crop Science, volume 17, issue 1, 2009, Pages 68~74
Vitis amurensis (VA; Vitaceae) has long been used in oriental herbal medicine. It has been reported that roots and seeds of VA have anti-inflammatory and antioxidant effects. In the present study, the protective effect of ethanol extract from stems and leaves of VA on hydrogen peroxide (
)-induced neuronal cell damage was examined in primary cultured rat cortical neurons. VA (10-100
/ml) concentration-dependently inhibited
-induced apoptotic neuronal cell death measured by 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and Hoechst 33342 staining. VA inhibited
-induced elevation of intracellular
) and generation of reactive oxygen species (ROS), which were measured by fluorescent dyes. Pretreatment of VA also prevented glutamate release into medium induced by 100
, which was measured by HPLC. These results suggest that VA showed a neuroprotective effect on
-induced neuronal cell death by interfering with
-induced elevation of
, glutamate release, and ROS generation. This has a significant meaning of finding a new pharmacological activity of stems and leaves of VA in the CNS.