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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Journal of Medicinal Crop Science
Journal Basic Information
Journal DOI :
The Korean Society of Medicinal Crop Science
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Volume & Issues
Volume 19, Issue 6 - Dec 2011
Volume 19, Issue 5 - Oct 2011
Volume 19, Issue 4 - Aug 2011
Volume 19, Issue 3 - Jun 2011
Volume 19, Issue 2 - Apr 2011
Volume 19, Issue 1 - Feb 2011
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Genetic Diversity and Relationship Analysis of Taraxacum officinale Weber and Taraxacum coreanum Nakai Accessions Based on Inter-Simple Sequence Repeats (ISSR) Markers
Ryu, Jai-Hyunk ; Bae, Chang-Hyu ;
Korean Journal of Medicinal Crop Science, volume 19, issue 3, 2011, Pages 149~156
DOI : 10.7783/KJMCS.2011.19.3.149
The genetic diversity and the genetic relationship among 30 genetic resources of T. officinale and T. coreanum collected from 20 regions in Korea were evaluated by using ISSR markers. Out of 127 loci detected overall, 122 were identified to be polymorphic with a rate of 96.0% at the 30 individuals. The intraspecific polymorphism between T. officinale and T. coreanum was 92.6% and 88.2%, respectively. The genetic similarity matrix (GSM) revealed a wide range of variablility among the 30 accessions, spanning from 0.179 to 922. According to the clustering analysis, different species T. officinale and T. coreanum, were divided into independent groups and all of the accessions could be classified into 7 categories. Especially, all of the mountain collected accessions belonged to independent groups. The study findings indicate that T. officinale and T. coreanum accessions have a high genetic diversity and accordingly carry a germ-plasm qualifying as good genetic resources for breeding.
Comparison of Growth Characteristics and Ginsenoside Content of Ginseng (Panax ginseng C. A. Meyer) Cultivated with Greenhouse and Traditional Shade Facility
Lee, Sung-Woo ; Kim, Gum-Sook ; Hyun, Dong-Yun ; Kim, Yong-Burm ; Kim, Jang-Wook ; Kang, Seung-Won ; Cha, Seon-Woo ;
Korean Journal of Medicinal Crop Science, volume 19, issue 3, 2011, Pages 157~161
DOI : 10.7783/KJMCS.2011.19.3.157
Growth characteristics, root yield and ginsenoside contents of 3-year-old ginseng in greenhouse shaded by
sloped-curtain made of aluminum were compared to traditional shade facility in order to develop cultural practice for organic ginseng. Light transmittance ratio in greenhouse with
sloped-curtain shade was distinctly lower than that of traditional shade from sunrise to 9 a.m., while its ratio in greenhouse was higher than traditional shade since 9 a.m. due to the reflection of light. Air temperature of greenhouse was
higher than that of traditional shade on the first ten days of August due to more reflected light. Root yield of greenhouse was 44% higher than that of traditional cultivation because of the inflow of reflected light and the decrease of disease of Alternaria and Anthracnose by blocking rainfall. Dry matter partitioning ratio of rhizome and lateral root were increased in ginseng cultivated at greenhouse due to longer survival time in leaf than traditional cultivation. Total ginsenoside contents cultivated at greenhouse was decreased in the part of taproot, while it was increased in the part of lateral and fine root compare to traditional cultivation. Individual ginsenoside contents between greenhouse and traditional cultivation showed significant difference more frequent in fine root than taproot and lateral root. Total ginsenoside contents including
, Rc, Rd, Re, Rf,
in whole root of 3-year-old ginseng did not showed significant difference by greenhouse and traditional cultivation.
Development of SCAR Markers for the Authentication of Acori Rhizoma Based on the Analysis of RAPD and Multiplex-PCR
Moon, Byeong-Cheol ; Ji, Yun-Ui ; Lee, Young-Mi ; Chun, Jin-Mi ; Lee, A-Yeong ; Choo, Byung-Kil ; Kim, Ho-Kyoung ;
Korean Journal of Medicinal Crop Science, volume 19, issue 3, 2011, Pages 162~169
DOI : 10.7783/KJMCS.2011.19.3.162
The rhizomes and herbal medicines originating from Acorus gramineus, A. calamus, A. tatarinowii, and A. gramineus var. pusilus, show significant similarity, and the correct identification of species is very difficult. Random Amplified Polymorphic DNA (RAPD) and Sequence Characterized Amplified Region (SCAR) were used to develop a reliable method for identification of these four species. Several distinct SCAR markers were developed from species-specific RAPD amplicons for each species. Furthermore, a useful molecular marker was established for multiplex-PCR, in order to the four species could be distinguished concurrently. These markers allow efficient and rapid identification of closely-related Acorus species and will be useful for standardization of herbal medicines.
Inhibitory Effect of Chrysanthemum zawadskii var. latilobum Kitamura Extracts against Mutagenicity of Cigarette Smoke Condensates (CSC)
Lee, Jin-Hee ; Lim, Heung-Bin ;
Korean Journal of Medicinal Crop Science, volume 19, issue 3, 2011, Pages 170~176
DOI : 10.7783/KJMCS.2011.19.3.170
This study was carried out to investigate whether Chrysanthermum zawadskii var. latilobum Kitamura (C. zawadskii) extracts has an inhibitory effect against the mutagenicity by cigarette smoke condensates (CSC). C. zawadskii was extracted with 70% ethanol and the yield was 18.5%. We further fractioned 70% ethanol extract sequentially to diethylether, chloroform, dichloromethane, and aqueous water, and gained the yield of 17.5%, 5.6%, 5.8%, 32.8% and 35.5%, respectively. In the Ames test, there was no mutagenic effect of crude extract and its solvent fractions up to 2 mg/plate toward Salmonella typhimurium TA 98 with or without S-9 mix metabolic activations. On the contrary, the crude extract showed an inhibitory activity against the mutagenicity of CSC in the presence of S-9 mix metabolic activation. Diethyl ether layer among five solvent fractions showed the highest inhibitory activity. The inhibitory activity of diethyl ether fraction was also increased in a dose-dependent manner and the inhibitory rate was about 97.7% at the concentration of 1 mg/plate. In this study, we conclude that crude extract of C. zawadskii itself is potentially safe for mutagenicity, and the diethyl ether fraction has an inhibitory effect against the mutagenicity of CSC.
Antioxidant and Whitening Effects of Agrimonia pilosa Ledeb Water Extract
Kim, Tae-Hyuk ; Kim, Jeong-Mi ; Baek, Jong-Mi ; Kim, Tae-Woo ; Kim, Dae-Jung ; Park, Jeong-Hae ; Choe, Myeon ;
Korean Journal of Medicinal Crop Science, volume 19, issue 3, 2011, Pages 177~184
DOI : 10.7783/KJMCS.2011.19.3.177
This study was performed to assess the antioxidant activities and whitening effects of Agrimonia pilosa Ledeb on melanin synthesis. The whitening effects of Agrimonia pilosa Ledeb water extracts were examined by in vitro mushroom tyrosinase assay and B16BL6 melanoma cells. We assessed inhibitory effect of Agrimonia pilosa Ledeb water extract on expression of melanogenic enzyme proteins including tyrosinase, tyrosinase-related protein 1 (TRP-1) and tyrosinase-related protein 2 (TRP-2) in B16BL6 cells. Inhibitory effect of Agrimonia pilosa Ledeb onto free radical generation was determined by measuring DPPH and hydroxyl radical scavenging activitie. Our results indicated that Agrimonia pilosa Ledeb water extract effectively inhibited free radical generation. In DPPH and hydroxy radical scavenging activity, Agrimonia pilosa Ledeb water extract had a potent anti-oxidant activity in a dose-dependent manner. They significantly inhibited tyrosinase activity in vitro and in B16BL6 melanoma cells. Also, Agrimonia pilosa Ledeb suppressed the expression of tyrosinase in B16BL6 melanoma cells. These results show that Agrimonia pilosa Ledeb inhibited melanin production on the melanogenesis. The underlying mechanism of Agrimonia pilosa Ledeb on whitening activity may be due to the inhibition of tyrosinase activity. We suggest that Agrimonia pilosa Ledeb may be useful as new natural active ingredients for antioxidant and whitening cosmetics.
Development of SSR Markers for Identification of Korean Ginseng (Panax ginseng C. A. Meyer) Cultivars
Bang, Kyong-Hwan ; Chung, Jong-Wook ; Kim, Young-Chang ; Lee, Jei-Wan ; Jo, Ick-Hyun ; Seo, A-Yeon ; Kim, Ok-Tae ; Hyun, Dong-Yun ; Kim, Dong-Hwi ; Cha, Seon-Woo ;
Korean Journal of Medicinal Crop Science, volume 19, issue 3, 2011, Pages 185~190
DOI : 10.7783/KJMCS.2011.19.3.185
The principal objective of this study was to develop a discrimination method using SSR markers in Korean ginseng cultivars. Five cultivars--Chunpoong, Yunpoong, Gopoong, Sunpoong, and Kumpoong--were evaluated by nine markers out of 22 SSR markers. A total of 23 alleles were detected, ranging from 1 to 4, with an average of 2.6 alleles per locus, and an averages of gene diversity (GD) of 0.480. Nine markers were tested in order to distinguish among five Korean ginseng cultivars. Two markers out of nine SSR markers, GB-PG-065 and GB-PG-142, were selected as key markers for discrimination among Korean ginseng cultivars. Two genotypes were detected in GB-PG-065. Chunpoong and Kumpoong shared the same allele type, and Yunpoong, Gopoong, and Sunpoong shared another identical allele type. In the case of GB-PG-142, a specific allele type differentiated from those of other four cultivars was observed only in Sunpoong cultivar. Consequently, the SSR markers developed in this study may prove useful for the identification of Korean ginseng cultivars and the development of ginseng seed management systems, as well as tests to guarantee the purity of ginseng seeds.
Enhancement of Antioxidant Activities and Whitening Effect of Acer mono Sap Through Nano Encapsulation Processes
Kim, Ji-Seon ; Seo, Yong-Chang ; Choi, Woon-Yong ; Kim, Hack-Soo ; Kim, Bo-Hyeon ; Shin, Dae-Hyeon ; Yoon, Chang-Soon ; Lim, Hye-Won ; Ahn, Ju-Hee ; Lee, Hyeon-Yong ;
Korean Journal of Medicinal Crop Science, volume 19, issue 3, 2011, Pages 191~197
DOI : 10.7783/KJMCS.2011.19.3.191
In this study, we investigated antioxidant activities and whitening effects of Acer mono sap by encapsulation of nanoparticles. Acer mono sap was through ultra high pressure process and then encapsulated by lecithin. Nano-encapsulated The nanoparticles of Acer mono sap showed highest free radical scavengering effect as 89.7% in adding sample (1.0 mg/ml), compared to sap of non-encapsulation. It was showed strong inhibition effect on melanin production test by Clone M-3 cells as 47.8%. High inhibitory of tyrosinase was also measured as 85.8% by adding lecithin nano-particle of 1.0 mg/ml. The nano-particles also showed 14.8% of low cytotoxicity against human normal fibroblast cells in adding 1.0 mg/ml of the highest concentration. These results indicate that Acer mono sap may be a source of cosmetic agents capable of improving whitening effect and antioxidant activites.
Enhancement of Antifungal Activity of Anthracnose in Pepper by Nanopaticles of Thiamine Di-lauryl Sulfate
Seo, Yong-Chang ; Cho, Jeong-Sub ; Jeong, Hae-Yoon ; Yim, Tae-Bin ; Cho, Kyoung-Sook ; Lee, Tae-Woo ; Jeong, Myoung-Hoon ; Lee, Gang-Hyeong ; Kim, Sung-Il ; Yoon, Won-Byung ; Lee, Hyeon-Yong ;
Korean Journal of Medicinal Crop Science, volume 19, issue 3, 2011, Pages 198~204
DOI : 10.7783/KJMCS.2011.19.3.198
This study was performed to enhance antifungal activity of anthracnose in chili pepper by nanopaticles of thiamine di-lauryl sulfate (TDS) through high pressure homogenization process. Yield of TDS was 79.14% by reaction of thiamine hydrochloride and sodium lauryl sulfate. TDS nanopaticle solution was manufactured through high pressure homogenization process. The turbidity of nanoparticles solution was increased with increasing the concentration of TDS, and nanoparticles solution of 100 ppm was showed the highest turbidity with absorbance of 3.212. The size of nanoparticles solution was measured as average 258.6 nm by DLS. Nanoparticles solution of 100 ppm showed growth inhibition activity with higher than about 80% compared to the control group against Colletotrichum gloeosporioides. Finally, nanoparticles solution was increased effectively the penetration of the TDS nanopaticles on attached cell membrane of hyphae and started to destruct the cells under microscope observation. Consequently, we suggested that the TDS nanoparticle solution by high pressure homogenization process might be suitable biochemical pesticides for improving the antifungal activities against anthracnose in pepper.