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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Journal of Medicinal Crop Science
Journal Basic Information
Journal DOI :
The Korean Society of Medicinal Crop Science
Editor in Chief :
Volume & Issues
Volume 20, Issue 6 - Dec 2012
Volume 20, Issue 5 - Oct 2012
Volume 20, Issue 4 - Aug 2012
Volume 20, Issue 3 - Jun 2012
Volume 20, Issue 2 - Apr 2012
Volume 20, Issue 1 - Feb 2012
Selecting the target year
Comparative Analysis of Terpenoids in in vitro Culture Media of Metabolically Engineered Transgenic and Wild Type Spearmint (Mentha spicata L.)
Kang, Young-Min ; Park, Dong-Jin ; Song, Hyun-Jin ; Ma, Ho-Seop ; Karigar, Chandrakant ; Choi, Myung-Suk ;
Korean Journal of Medicinal Crop Science, volume 20, issue 5, 2012, Pages 301~307
DOI : 10.7783/KJMCS.2012.20.4.301
IPP isomerase (Iso) and Limonene synthase (Limo) are important enzymes in terpenoids biosynthesis pathway. The wild type and each metabolically engineered (Iso and Limo) transgenic spearmint (Mentha spicata Linne) plants were compared for their growth patterns and the contents of essential oil in in vitro culture media. The profile of terpenoid metabolites was obtained from the essential oil of the metabolically engineered transgenic spearmint, which was extracted using a modified SDE method, by GC-MS analysis. The growth of wild spearmint was more profuse in B5 culture medium than in other media. Significant differences in leaf and root growth patterns were observed between metabolically engineered transgenic and wild type spearmint plants. The leaves of the transgenic spearmint plants were slightly elongated but were dramatically narrower than those of wild type spearmints. The content of essential oil of transgenic spearmint was different slightly depending on the target terpenoid genes. The content of essential oils in Limo transgenic plants was higher than that of Iso, except for transgenic plant in B5 medium. The transgenic spearmint produced more terpenoids than the wild type. Iso spearmint extracts showed eleven terpenoids and a phenylpropane, while Limo spearmint extracts contained nine terpenoids. However, extracts from the wild type showed the presence of only four terpenoids.
Antibacterial Activity and Nitric Oxide Production Inhibitory Activity of the Extract and its Fractions from the Leaves of Prunus sargentii
Yang, Sun-A ; Pyo, Byoung-Sik ; Kim, Sun-Min ; Lee, Kyoung-In ;
Korean Journal of Medicinal Crop Science, volume 20, issue 5, 2012, Pages 308~314
DOI : 10.7783/KJMCS.2012.20.4.308
This study was carried out to investigate the antibacterial activity against pathogens of acne and the anti-inflammatory effect of 75% ethanol extract and its fractions from the leaves of Prunus sargentii. In the antibacterial activity by the disc diffusion assay, the extract showed the highest effect against Propionibacterium acnes, Staphylococcus aureus and Staphylococcus epidermidis in 5 mg/disc. However, the ethyl acetate fraction showed the highest antibacterial activity in 1 mg/disc. On the other hand, the hexane and chloroform fraction showed strong nitric oxide (NO) production inhibitory effect in lipopolysaccharide (LPS)-stimulated Raw 264.7 cell. In the cell viability of Raw 264.7 by MTT assay, the extract and all fractions were exhibited normal viabilities as nontoxic result. Consequently, the extract from the leaves of P. sargentii and its ethyl acetate fraction could be applicable to functional materials for antibacterial activity related fields. Moreover, the hexane and chloroform fraction could be applicable to candidate materials as anti-inflammatory agent.
Effects of Plant Growth Regulator Treatment on Ginseng berry and seed development in Panax ginseng C. A. Meyer
Jo, Seo-Ri ; Kim, Jung-Sun ; Lee, Nu-Ri ; Choi, Jae-Eul ;
Korean Journal of Medicinal Crop Science, volume 20, issue 5, 2012, Pages 315~319
DOI : 10.7783/KJMCS.2012.20.5.315
This study was conducted to select plant growth regulators effective at ginseng berry set inhibition to help root growth in Korean ginseng (Panax ginseng C. A. Meyer). PGRs (ethephon, gibberellic acid, maleic hydrazide, coumarin) were applied to field grown 5-year-old Korean ginseng between one and two times, before and during bloom in 2009, 2010. The number of treatment was more effective in ginseng berry set inhibition when used two times compared with one time in GA 1,000 ppm, MH (5,000, 10,000 ppm), coumarin (5,000, 10,000 ppm) treatment. According to treatment period of plant growth regulator, ginseng berry set inhibition rate from 20days before flowering date to 5days after blooming was the highest in MH 5000 ppm showing 99.9% and the lowest in GA 100 ppm showing 32.8%. The spray treatments of Ethephon (50, 150 ppm) and MH (5,000, 10,000 ppm) from 20 days before the flowering bloom up to 5 days before, and coumarin (5,000, 10,000 ppm) from 20 days to 6 days and before blooming that induced the inhibitory effect more than 90% after 12 weeks. Considering ginseng berry set inhibition characteristics and treatment period ethephon and coumarin was important about applied period but, MH treatment appeared to effective ginseng berry set inhibition regardless of treatment period.
Changes of Characteristics Related to Photosynthesis in Synurus deltoides under Different Shading Treatments
Lee, Kyeong-Cheol ; Noh, Hee-Sun ; Kim, Jong-Whan ; Ahn, Soo-Yong ; Han, Sang-Sup ;
Korean Journal of Medicinal Crop Science, volume 20, issue 5, 2012, Pages 320~330
DOI : 10.7783/KJMCS.2012.20.4.320
This study was conducted to investigate the changes of chlorophyll contents, chlorophyll fluorescence, photosynthetic parameters, and leaf growth of Synurus deltoides under different shading treatments. S. deltoides was grown under non-treated (full sunlight) and three different shading conditions (Shaded 88~93%, 65~75%, and 45%~55%). Light compensation point (
), dark respiration (
), maximum photosynthesis rate (
), photo respiration rate (
), carboxylation efficiency (
), and photochemical efficiency were decreased with increasing shading level; However,
compensation point (
), total chlorophyll content, and specific leaf area (SLA) were shown the opposite trend. S. deltoides under 88~93% treatment showed the lowest photosynthetic activity such as maximum photosynthetic rate (
), photochemical efficiency, and
compensation point (
). Therefore, photosynthetic activity will be sharply decreased with a long period of 8~12% of full sunlight. With the shading level decreased, carotenoid content and non-photochemical fluorescence quenching (NPQ) increased to prevent excessive light damage. This result suggested that growth and physiology of S. deltoides adapted to high light intensity through regulating its internal mechanism.
Sensory Characteristics of Granular Tea and the Components of Mulberry Fruit Extracts by Different Extraction Process
Ryu, Il-Hwan ; Kwon, Tae-Oh ;
Korean Journal of Medicinal Crop Science, volume 20, issue 5, 2012, Pages 331~338
DOI : 10.7783/KJMCS.2012.20.5.331
In the present work, mulberry fruit extracts by four extraction processes, namely wet pressing extraction (WPE), hot-water extraction (HWE), enzymatic hydrolysis (EH), and lactic-acid bacteria fermentation (LBF) by Lactobacillus plantarum TO-2100, were analyzed for nutrients and functional compounds. The sugar contents of extracts by WPE, HWE, EH, and LBF were 12.0, 10.9, 14.5, and 14.3 brix, respectively, and the extraction yields by EH and LBF were 1.65 and 1.50 times higher than those by WPE. Among the organic acids, tartaric acid and malic acid contents were the highest in the extracts by WPE. Acetic acid was best extracted by LBF, and citric acid was best extracted by EH. Lactic acid was detected only in LBF. The extracts by EH showed the highest contents of all vitamins with an exception that the extracts by LBF showed the highest contents of the folic acid, vitamin B12, and vitamin C. We also noted that vitamin B group was not detected in the extracts by LBF. The extracts by EH showed the highest contents of all the amino acids, whereas LBF showed the lowest. Polyphenol contents of extracts by EH and LBF were 3.05 and 2.51 times more than those by WPE respectively. Anthocyanin contents were 7.66, 7.14 times higher for EH and LBF compare to WPE. We manufactured mulberry fruit granular teas with different compositions and tested them for their sensory characteristics. We found that 15% mulberry fruit extracts by enzymatic hydrolysis and 85% dextrin composition gave the most satisfactory result.
Screening of Antifungal Bacillus spp. against Alternaria Blight Pathogen (Alternaria panax) and Anthracnose Pathogen (Colletotrichum gloeosporioides) of Ginseng
Lee, Hye-Jin ; Park, Kee-Choon ; Lee, Seung-Ho ; Bang, Kyong-Hwan ; Park, Hong-Woo ; Hyun, Dong-Yun ; Kang, Seung-Weon ; Cha, Seon-Woo ; Chung, Ill-Min ;
Korean Journal of Medicinal Crop Science, volume 20, issue 5, 2012, Pages 339~344
DOI : 10.7783/KJMCS.2012.20.5.339
This study was carried out to research microorganisms having the antifungal activity against ginseng Alternaria blight pathogen Alternaria panax and ginseng anthracnose pathogen Colletotrichum gloeosporioides. Eleven Bacillus strains. were isolated from Korean traditional soybean paste and Kimchi. Among the 11 isolates, DJ5, KC1, KC2 and KC4 showing antagonistic activity on the mycelial growth of A. panax and C. gloeosporioides in pairing culture were finally selected as the antagonistic microorganisms. Based on 16s rRNA sequence and phylogenetic tree analysis, they were identified as Bacillus spp.. The selected microorganisms were investigated antagonistic activity by measured leaf-segment colonization in pot test. When Bacillus sp. were injected after A. panax treatment, KC1, KC2 and KC4 showed similar effect to chemical pesticides treated control. To measure preventive effect of Bacillus sp, antagonistic microorganisms were injected and C. gloeosporioides was treated in pot. When measuring the effectiveness for the prevention of Anthracnose, All Bacillus spp. showed approximately 83~90 % degree of superior preventive effect. In general, The four Bacillus spp. isolated from Korean traditional fermented foods showed therapeutic effect of Alternaria blight and preventive effect of Anthracnose.
Effect of Root Extract of Lythrum salicaria L. on Liver Function of Rat Acutely Administrated with Alcohol
Lee, Seung-Eun ; Lee, Jeong-Hoon ; Kim, Geum-Soog ; Hong, Yoon-Pyo ; Noh, Hyung-Jun ; Park, Chun-Geun ; Kim, Seung-Yu ;
Korean Journal of Medicinal Crop Science, volume 20, issue 5, 2012, Pages 345~352
DOI : 10.7783/KJMCS.2012.20.5.345
This experiment was conducted to verify whether one time feeding of Lythrum salicaria root crude extract (LSR extract) exhibits liver protecting activities in acutely ethanol administrated rat. Experiment groups were composed of normal, negative control (alcohol control), 2 positive control (Hovenia dulcis extract 900 mg/kg and milk thisle 100 mg/kg), betulinic acid (20 mg/kg), a compound separated from LSR, and 3 LSR (100, 300, 900 mg/kg) groups. LSR treated groups showed decrease (p < 0.05) in serum triglyceride by dose-dependent manner. The content of serum albumin and the activity of ADH and ALDH in LSR extract fed rats were increased (p < 0.05) dependently on the administration amounts. Our study indicated that one time supplement of LSR downregulates oxidative stress and shows liver protective activity in the acute alcohol-fed rats.
Effects of Cutting Date, Shading Ratio and Growth Regulator on Rooting of Acanthopanax divaricatus var. albeofructus and Acanthopanax koreanum Nakai
Lee, Jung-Jong ; Lee, Sang-Hyun ; Seo, Pil-Dae ; Park, Chun-Geun ; Lee, Sang-Chul ;
Korean Journal of Medicinal Crop Science, volume 20, issue 5, 2012, Pages 353~358
DOI : 10.7783/KJMCS.2012.20.5.353
This study was conducted to enhance the propagation of Acanthopanax divaricatus and Acanthopanax koreanum through different cutting practices. For propagation by cuttings, scions were taken on September 1. This already has hard skin and showed much higher values in terms of root length, root number, rooting ratio and root weight than those taken on June 30 and August 1. Regardless of cutting date, 50% shading resulted to the highest root length, root number, root ratio and root weight. Meanwhile, 95% shading significantly reduced these parameters compared with no shading. These results suggest that over-shading may inhibit root growth. Two growth regulators, IBA (concentration of 1,000, 2,000, 3,000 ppm) and Rootone-F (0.4%) were also tested of its effect to the cuttings. Rootone-F was found to be more effective than IBA. Cutting treated with Rootone-F had slightly higher root length and root number. A. koreanum which grows well in hot climatic condition showed better rooting ability than A. divaricatus.
Variation of Bolting at Cultivation of Different Regions and Molecular Characterization of FLC homologs in Angelica gigas Nakai
Kim, Young-Guk ; Yeo, Jun-Hwan ; An, Tae-Jin ; Han, Sin-Hee ; Ahn, Young-Sup ; Park, Chung-Beom ; Jang, Yun-Hee ; Kim, Jeong-Kook ;
Korean Journal of Medicinal Crop Science, volume 20, issue 5, 2012, Pages 359~364
DOI : 10.7783/KJMCS.2012.20.5.359
This study were carried out to find bolting response of cultivation in different regions and to isolate FLC (FLOWERING LOCUS C) homologs in Angelica gigas Nakai. The mean temperature of different regions, ordering in altitude, were as follows: 100 m > 350 m > 530 m > 700 m. The largest amount of rainfall was occurred in the region of 350 m while the longest time of sunshine was occurred in the region of 100 m. The content of soil chemical properties in regions showed pH 6.2 ~ 7.4, T-N 0.17 ~ 26, organic mater
, exchangeable potassium and calcium and magnesium were 0.78 ~ 1.15, 3.9 ~ 10.0,
. L5 line of A. gigas was occurred in bolting at all regions, but the bolting ratio was 60.0% in 700 m region with non-mulching treatment. Manchu of A. gigas was not occurred in bolting at all regions. The accumulation bolting ratio of L5 line by non-mulching was higher than that of mulching as 90.4% and 72.8% in 100 m region. The MADS-box transcription factor FLC is one of the well-known examples as a strong floral repressor. We decided to isolate FLC homologs from A. gigas as a starting point of flowering mechanism research of this plant. We have isolated two RT-PCR products which showed very high amino acid sequence homology to Arabidopsis FLC.
Study on Flowering, Bearing Fruit, Seed Harvesting and Seedling Transplanting Cultivation of Valeriana fauriei Briquet
Ahn, Young-Sup ; Hur, Mok ; An, Tae-Jin ; Park, Chun-Geun ; Kim, Young-Guk ; Park, Chung-Berm ; Baek, Wan-Sook ;
Korean Journal of Medicinal Crop Science, volume 20, issue 5, 2012, Pages 365~371
DOI : 10.7783/KJMCS.2012.20.5.365
This study was carried out to know the characteristics of flowering and bearing fruit, the optimum period, regions and methods for seed harvesting, the optimum temperatures for seed storage and germination, and the optimum period for sowing at nursery bed and seedling transplanting of Valeriana fauriei Briquet. The flowering and bearing fruit of Valeriana fauriei was developed from the before-year root. Optimum period for seed harvest of Valeriana fauriei was from late July to middle August, and optimum areas were the high elevated areas over 500 m above the sea level as Jinbu-myeon, Pyeongchang-gun, Gangwon-do. Using of net-bag for seed harvesting was the effective method to gather the full ripe seed, and bagging of net-bag was necessary from the season of middle May that was the flowering middle-stage. Germination rates don`t show the difference among the different temperatures of storage as approximately 41% at
of seed storage temperatures. The optimum temperature range was in
for seed germination at nursery bed. The optimum period for seed sowing at nursery bed was the late February, and the optimum period for seedling transplanting was the middle April.
Variation of Astragalosides Contents in Cultivated Astragalus membranaceus
Kim, Seung-Hyun ; Jun, Yoon-Mi ; Lim, Ju-Jin ; Kim, Sung-Hyop ; Chung, Ill-Min ; Kim, Eun-Hye ;
Korean Journal of Medicinal Crop Science, volume 20, issue 5, 2012, Pages 372~380
DOI : 10.7783/KJMCS.2012.20.5.372
This study was conducted to determine the contents of astragalosides in Astragalus membranaceus. The total astragaloside content (
) of 2 year-cultivated astragalus was the highest among the variously cultivated astragalus plants. Upon an increase in cultivation time, the average value of the total astragaloside content decreased (from
). Especially, the content of astragaloside decreased rapidly. Comparison between the top and subterranean parts of harvested astragalus plants showed that as the cultivation time increased, the astragaloside content of the top part decreased. On the other hand, the subterranean part demonstrated the opposite pattern. The astragaloside content of the top root increased compared to that in the lateral root (from
). Further, the content of astragaloside increased in the top root as the cultivation time increased.
Anti-inflammatory Effects of Water Extract of Eucommia ulmoides OLIVER on the LPS-induced RAW 264.7 Cells
Kim, Young-Ock ; Lee, Sang-Won ; Sohn, Sang-Hyun ; Kim, Seung-Yu ; Oh, Myung-Sook ; Kim, Su-Kang ;
Korean Journal of Medicinal Crop Science, volume 20, issue 5, 2012, Pages 381~386
DOI : 10.7783/KJMCS.2012.20.5.381
Eucommia ulmoides OLIVER (EU) is a traditional Korean herbal used for the treatment of rheumatoid arthritis (RA). In the present study, the molecular pharmacology basis of its anti-inflammatory effect is revealed in this work, EU was studied in lipopolysaccharide (LPS)-activated macrophage cells (RAW 264.7) as an established inflammation model. After activation, nitric oxide (NO) production and iNOS mRNA were measured by using a colorimetric assay (Griess reagent), and reverse transcription polymerase chain reaction (RT-PCR), respectively. The change in the content of
, and IL-6 was concurrently monitored by ELISA. In results, we found that in the concentration range without showing cytotoxicity, EU produced a remarkable anti-inflammatory effect and showed a dose-dependent inhibition of LPSinduced NO production. Compared with indomethacin, EU has more potency and a specific action of NO inhibition,
, IL-6, and TNF-
inhibition. These results suggest that EU may be a suitable herbal medicine to yield the greatest anti-inflammatory activity for food additives and medicine.
Molecular Identification of Korean Ginseng Cultivars (Panax ginseng C. A. Mey.) using Peptide Nucleic Acid (PNA) Microarray
Bang, Kyong-Hwan ; Jo, Ick-Hyun ; Kim, Young-Chang ; Kim, Jang-Uk ; Park, Hong-Woo ; Shin, Mi-Ran ; Kim, Young-Bae ; Kim, Ok-Tae ; Hyun, Dong-Yun ; Kim, Dong-Hwi ; Cha, Seon-Woo ;
Korean Journal of Medicinal Crop Science, volume 20, issue 5, 2012, Pages 387~392
DOI : 10.7783/KJMCS.2012.20.5.387
This study was carried out to identify Korean ginseng cultivars using peptide nucleic acid (PNA) microarray. Sixty-seven probes were designed based on nucleotide variation to distinguish Korean ginseng cultivars of Panax ginseng. Among those PNA probes, three (PGB74, PGB110 and PGB130) have been developed to distinguish five Korean ginseng cultivars. Five Korean ginseng cultivars were denoted as barcode numbers depending on their fluorescent signal patterns of each cultivar using three probe sets in the PNA microarray. Five Korean ginseng cultivars, Chunpoong, Yunpoong, Gopoong, Gumpoong and Sunpoong, were simply denoted as `111`, `222`, `211`, `221` and `122`, respectively. This is the first report of PNA microarray which provided an objective and reliable method for the authentication of Korean ginseng cultivars. Also, the PNA microarray will be useful for management system and pure guarantee in ginseng seed.
The Germination Characteristics of Rheum undulatum L. Seeds in Treatment Conditions
Yoo, Ji-Hye ; Hwang, In-Seong ; Seong, Eun-Soo ; Lee, Jae-Geun ; Kim, Hee-Young ; Kim, Nam-Jun ; Lim, Jung-Dae ; Ham, Jin-Kwan ; Ahn, Young-Sup ; Yu, Chang-Yeon ;
Korean Journal of Medicinal Crop Science, volume 20, issue 5, 2012, Pages 393~397
DOI : 10.7783/KJMCS.2012.20.5.393
This study was investigated seed germination of Rheum undulatum L. depends on storage temperature (-20,
and room temperature), germination temperature (5, 15, 25 and
) and a soaking treatment (no soaking, 2 days and 4 days). There was not much difference from the germination rate of R. undulatum L. in storage temperature conditions for 8 weeks. The highest of germination rate occurred over 80 percentage at
. When R. undulatum L. was soaked with tap water for four days, the average values of germination velocity and germination day were represented of 1.7 ea/day and 1.68 days. These results may help our understanding of variations in germination characteristics for R. undulatum L. seeds treated under various germination conditions.