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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Journal of Medicinal Crop Science
Journal Basic Information
Journal DOI :
The Korean Society of Medicinal Crop Science
Editor in Chief :
Volume & Issues
Volume 21, Issue 6 - Dec 2013
Volume 21, Issue 5 - Oct 2013
Volume 21, Issue 4 - Aug 2013
Volume 21, Issue 3 - Jun 2013
Volume 21, Issue 2 - Apr 2013
Volume 21, Issue 1 - Feb 2013
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Buckwheat Extract Increases Resistance to Oxidative Stress and Lifespan in Caenorhabditis elegans
Kim, Chul Kyu ; Park, Sang Kyu ;
Korean Journal of Medicinal Crop Science, volume 21, issue 1, 2013, Pages 1~6
DOI : 10.7783/KJMCS.2013.21.1.1
Buckwheat (Fagopyrum esculentum) has been known for having strong anti-oxidant, anti-mutagenic, and anti-carcinogenic activities. The free radical theory of aging, also known as the oxidative stress theory of aging, claims that cellular oxidative damage accumulated with time is a major causal factor of aging. In the present study, we investigated the effect of buckwheat extracts on resistance to oxidative stress and aging using Caenorhabditis elegans as a model system. Survival under an oxidative-stress condition induced by paraquat increased markedly following 500mg/L buckwheat extracts treatment, suggesting lower cellular oxidative damage by buckwheat extracts. A lifespan assay also revealed that treatment of buckwheat extracts significantly extended both the mean and maximum lifespan in C. elegans. Interestingly, this lifespan-extension by buckwheat extracts was not accompanied by reduced fertility. These findings suggest that buckwheat extracts can confer longevity phenotype to C. elegans through its strong anti-oxidant activity and support the aging theory which emphasizes a pivotal role of oxidative stress during aging.
Effect of Priming and Seed Pellet Technique for Improved Germination and Growth in Fraxinus rhynchophylla and Alnus sibirica
Park, Hae Il ; Shim, Hoon Seob ; Choi, Li Na ; Jo, Hyeon Gil ; Han, Seung Ho ; Lee, Jae Geun ; Yu, Chang Yeon ; Lim, Jung Dae ;
Korean Journal of Medicinal Crop Science, volume 21, issue 1, 2013, Pages 7~19
DOI : 10.7783/KJMCS.2013.21.1.7
This study was carried out to select new pelleting binder and material for seeds from Fraxinus rhynchophylla Hance and Alnus sibirica Fisch. ex Turcz. The optimum treatments of the various concentrations and species of priming agents to improve seed germination of both woody medicinal plants were also estimated. Germinability was increased when the seeds of Fraxinus rhynchophylla Hance was soaked in -1.0 MPa of PEG6000 solution at
for 4 days significantly, the optimum treatment for improving germination of Alnus sibirica Fisch. ex Turcz was observed when the tested seeds was soaked in 100 mM of KCl at
for 4 days. The influence of physical and chemical properties of pelleting solid materials, the mixture of gypsum, diatomaceous earth, dalma ceramic and vermicuolite (6:1:1:1 ratio) were found as the best pelleting materials for Fraxinus rhynchophylla Hance and Alnus sibirica Fisch. ex Turcz. seeds. To satisfy the requirements of absorption and compatibility for multi-layer seed pelleting, SGPA (Starch-grafted cross-linked polyacrylates) hydrogel was prepared using starch, acrylonitrile, ceric ammonium nitrate, nitric acid, methyl alcohol and potassium hydroxide. The resulting SGPA hydrogel showed high water absorption but not plant compatibility. It suggested that seed pelleting using pelleting materials and SGPA hydrogel (multi-layer coating) after priming agent treatment is to increase germinability and seedling growth and it can reduced irrigation labours and can also save seed.
Studies on Germination Rates and Early Seedling Growth Characteristics by Different Storing Temperatures, Durations, and Methods in Aralia cordata var. continentalis (Kitagawa) Y.C.Chu
Lee, Gyeong A ; Kim, Do Hyun ; Kim, Min Su ; Wu, Wen Guo ; Kim, Young Gook ; Ahn, Young Sup ; Park, Chung Beom ; Song, Beom Heon ;
Korean Journal of Medicinal Crop Science, volume 21, issue 1, 2013, Pages 20~26
DOI : 10.7783/KJMCS.2013.21.1.20
This study was conducted to improve the managing and storing methods of the seeds of Aralia cordata var. continentalis (Kitagawa) Y.C.Chu, to examine the viability and the germination ability of seeds with different storing conditions and methods, and to develop new ways to propagate and have better healthy seedling. Therefore, the germination rate, days required for germinating seeds, and early growth responses of Aralia cordata var. continentalis (Kitagawa) Y.C.Chu were investigated with different storing temperatures, durations and methods. The germination rate was higher in stratified storage than that in dry storage condition. The highest germination rate was with outdoor temperature at 30 days after stratified storage. The days required for germinating seeds were less than 10 days with the treatment of
and outdoor temperature in stratified storage. In dry condition, they were shorter with
than those with
and outdoor temperature. Leaf number of seedling was higher in stratified storage compared to that in dry condition, while it was not clearly different according to storage temperatures and durations. Leaf length and leaf width of seedling was not difference among the treatment of storage methods, temperatures, and durations. Stem length of seedling was higher in stratified storage than those in dry condition, while root length was not clearly different among the treatments. It would be assumed that temperatures, methods and durations of storage could affect much to the germination rate and the early seedling growth response.
Identification of Fungal Pathogen Causing Seedling Rot of Lithospermum erythrorhizon and Study on the Optimum Growing Temperature for Decreasing of the Seedling Rot
An, Tae Jin ; Shin, Kyu Seop ; Ahn, Young Sup ; Hur, Mok ; Park, Chung Berm ;
Korean Journal of Medicinal Crop Science, volume 21, issue 1, 2013, Pages 27~31
DOI : 10.7783/KJMCS.2013.21.1.27
This study was conducted to isolate and identify the fungal pathogen causing seedling rot of Lithospermum erythrorhizon Siebold & Zuccarini, and to know the optimum growing temperature for decreasing seedling rot of Lithospermum erythrorhizon. On the basis of morphological characteristics, EF-1a sequence analysis, and pathogenecity to host plant, the fungi isolated from seedling rot and seeds of Lithospermum erythrorhizon were identified as Fusarium fujikuroi, indicating that disease causing fungus is seed-borne pathogen. Optimum temperature for germination of seeds of Lithospermum erythrorhizon was
, but pathogenicity of Fusarium fujikuroi was shown more readily at
. These results suggested that seedling culture of Lithospermum erythrorhizon between
might reduce seedling rot of Lithospermum erythrorhizon caused by seed-borne pathogen Fusarium fujikuroi.
Overexpression of Farnesyl Diphosphate Synthase by Introducing CaFPS Gene in Panax ginseng C. A. Mey.
Park, Hong Woo ; Kim, Ok Tae ; Hyun, Dong Yun ; Kim, Yong Bum ; Kim, Jang Uk ; Kim, Young Chang ; Bang, Kyong Hwan ; Cha, Seon Woo ; Choi, Jae Eul ;
Korean Journal of Medicinal Crop Science, volume 21, issue 1, 2013, Pages 32~38
DOI : 10.7783/KJMCS.2013.21.1.32
FPS (farnesyl diphosphate synthase) plays an essential role in organ development in plants. However, FPS has not previously been identified as a key regulatory enzyme in triterpene biosynthesis. In order to investigate the effect of FPS on ginsenosides biosynthesis, we over-expressed FPS of Centella asiatica (CaFPS) in Panax giseng adventitious roots. PCR analysis showed the integrations of the CaFPS and hygromycin phosphotransferase genes and we ultimately selected three lines. The result of Southern blot analysis demonstrated the introduction of the CaFPS gene into genome of ginseng. In addition, the results of RT-PCR analysis revealed that CaFPS gene overexpression induced an accumulation of its transcription in the ginseng adventitious roots. To determine whether or not the overexpression of the CaFPS gene contributes to the downstream gene expression associated with triterpene biosynthesis, the level of mRNAs was analyzed by real-time PCR. The result showed that no differences were detected in any expression of all genes. To determine quantitatively the content of ginsenosides in transgenic ginseng adventitious roots, HPLC analysis was conducted. The content of total 7 ginsenosides was increased to 1.8, 1.4, and 1.7 times than that of the controls, respectively. This indicated that the overexpression of CaFPS in ginseng adventitious roots causes an increase in ginsenoside content, although down stream genes of FPS gene were suppressed by CaFPS overexpression.
Effect of Shading and Pinching on Growth and Acanthoside-D content of Acanthopanax divaricatus var. albeofructus and Acanthopanax koreanum Nakai
Lee, Jung Jong ; Lee, Sang Hyun ; Park, Jae Sang ; Ahn, Young Sup ; Lee, Sang Chul ;
Korean Journal of Medicinal Crop Science, volume 21, issue 1, 2013, Pages 39~44
DOI : 10.7783/KJMCS.2013.21.1.39
This study was conducted to identify the effect of shading and pinching on growth and acanthoside-D content of Acanthopanax divaricatus var. albeofructus and A. koreanum Nakai. Different pinching heights showed no significant differences in terms of plant growth and acanthoside-D content but higher values showed that pinching A. divaricatus at 60 cm and A. koreanum at 30 cm favored good growth and higher fresh weight in the shoots. The content of acanthoside-D was not significantly affected by pinching heights. Also, no significant difference in acanthoside-D content was found between the lower and upper part of plant in the first year. However it was much higher in the lower part than the upper part in the second year, which indicated that the content of acanthoside-D was comparatively high in the lower part where lignification is much advanced. Shading showed benefits in terms of growth of A. divaricatus while only 50%-shading was favorable for A. koreanum to achieve superior growth. Overall, results indicated that shading had favorably affected the growth of the 2 Acanthopanax species while no-shading is better if we opt to achieve higher acathoside D content.
Effects on the Application of Popped Rice Hulls Compost as Substitute Material of Yacto in Yang-jik Nursery Bed of Panax ginseng C. A. Meyer
Kang, Seung Weon ; Lee, Sung Woo ; Hyun, Dong Yun ; Kim, Jang Wook ; Kim, Yong Bum ; Lee, Hye Jin ; Cha, Seon Woo ;
Korean Journal of Medicinal Crop Science, volume 21, issue 1, 2013, Pages 45~48
DOI : 10.7783/KJMCS.2013.21.1.45
This study was carried out to know the substitute effect of Yacto (leaf mold compost) on popped rice hulls compost (PRHC) in Yang-jik nursery bed of Panax ginseng. PRHC was mixed with Yacto as 50:50 ratio, and 1 ~ 2% of the mixed oil cake, rice bran and urea were also added to promote decaying the mixed compost. The mixed compost made by PRHC and Yacto was showed that positive effect on the growth of ginseng seedling when it was mixed with 1% of oil cake and rice bran, and 2% of mixed oil cake. But addition to the 2% of urea in the mixture of PRHC and Yacto was not positive effect on the growth of ginseng seedling. Root yield of the mixed compost was similar to that of conventional compost by made 100% of Yacto. Therefore, the mixed compost can substitute for Yacto when PRHC and Yacto were mixed by 50:50 ratio and added 1% of oil cake and rice bran.
Adaptation of Farm Field Transplanting and Growth Habitat of Artemisia capillaris in Korea
Song, Hong Seon ; Kim, Seong Min ;
Korean Journal of Medicinal Crop Science, volume 21, issue 1, 2013, Pages 49~53
DOI : 10.7783/KJMCS.2013.21.1.49
This study was performed to evaluate the adaptability of farm field soil transplanting and habitat growth in inland and seashore areas of Artemisia capillaris in South Korea. In habitat, Artemisia capillaris was distributed both in the inland and seashore area as hemicryptophyte, and it grows individually on the slope of the open sunlight. The inland and seashore soils of habitat was the slightly acid and weakly alkaline, respectively. Plant height was 55.6 cm, and it was higher in inland than that of the seashore area. The stem and branch number was less in inland than that of the seashore area. Flowering period was mid-August, and flower of inland blossomed early 1 ~ 3 days than that of the seashore area. The flowering and fruiting rate was slightly lower in inland than that of the seashore area. After transplanting of Artemisia capillaris, plant height was 71.7 cm, and it was higher 16.1 cm than that of the habitat. The stem and branch number was more than that of the habitat, and flower blossomed early 3 ~ 4 days than that of the habitat. Transplanting survival rate was 85.1%, it was slightly higher in inland than that of the seashore area.
Screening of Skin Whitening Activity of Codonopsis lanceolata Extract by Complex Steaming Process
Kim, Ji Seon ; Kim, Ji Woong ; Kwon, Hee Seok ; Lim, Hye Won ; Lee, Hyeon Yong ;
Korean Journal of Medicinal Crop Science, volume 21, issue 1, 2013, Pages 54~60
DOI : 10.7783/KJMCS.2013.21.1.54
According to previous reports, antioxidant activities of Codonopsis lanceolata could be increased by a steaming process. This study was performed to improve its antioxidant activity and skin whitening activities of C. lanceolata by high pressure and stepwise steaming complex process. The complex processed C. lanceolata showed highest free radical scavenging acitivity as 45.21%, and for phenol and flavonoid contents, complex processed C. lanceolata contained higher than those from conventional extraction process or steaming process alone. The Cytotoxicity of all C. lanceolata extracts also showed low cytotoxicity against human fibroblast cell (CCD-986sk) as 4.49 ~ 10.40%. In whitening activity, high inhibition of tyrosinase activity was estimated as 25.08% by adding the extracts from complex process. We found that whitening and antioxidant activity of complex processed C. lancolata extract was higher than those obtained from conventional extraction and a steaming process because various kinds of antioxidant compounds could be easily released by combined process, compared to one of each process.