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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Journal of Medicinal Crop Science
Journal Basic Information
Journal DOI :
The Korean Society of Medicinal Crop Science
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Volume & Issues
Volume 21, Issue 6 - Dec 2013
Volume 21, Issue 5 - Oct 2013
Volume 21, Issue 4 - Aug 2013
Volume 21, Issue 3 - Jun 2013
Volume 21, Issue 2 - Apr 2013
Volume 21, Issue 1 - Feb 2013
Selecting the target year
Development of RAPD-Derived SCAR Markers and Multiplex-PCR for Authentication of the Schisandrae Fructus
Lee, Young Mi ; Moon, Byeong Cheol ; Ji, Yunui ; Seo, Hyeong Seok ; Kim, Ho Kyoung ;
Korean Journal of Medicinal Crop Science, volume 21, issue 3, 2013, Pages 165~173
DOI : 10.7783/KJMCS.2013.21.3.165
The fruits of Schisandra chinensis have been used as an edible ingredient and traditional medicine in Korea. Due to morphological similarities of dried mature fruits, the correct identification of S. chinensis from other closely related Schisandrae species is very difficult. Therefore, molecular biological tools based on genetic analysis are required to identify authentic Schisandrae Fructus. Random amplifed polymorphic DNA (RAPD) and Sequence Characterized Amplified Region (SCAR) were used to develop an easy, reliable and reproducible method for the authentication of these four species. In this paper, we developed several RAPD-derived species specific SCAR markers and established a multiplex-PCR condition suitable to discriminate each species. These genetic markers will be useful to distinguish and authenticate Schisandrae Fructus and four medicinal plants, S. chinensis, S. sphenanthera, S. repanda and K. japonica, in species level.
Effect of Spraying Lime-Bordeaux Mixture as Concentration and Appling Time Series on Growth and Disease Occurrence of Three-year-old Ginseng (Panax ginseng C. A. Meyer)
Jung, Won Kwon ; Ahn, Deok Jong ; Choi, Jin Kook ; Jang, Myeong Hwan ; Kwon, Tae Ryong ;
Korean Journal of Medicinal Crop Science, volume 21, issue 3, 2013, Pages 174~178
DOI : 10.7783/KJMCS.2013.21.3.174
Lime-bordeaux mixture has been used to prevent diseases in the field of ginseng. The purpose of this study is to investigate the efficacy of lime-bordeaux depending on the concentration and treatment time on major diseases of ginseng such as Alternaria blight and anthracnose, and to evaluate the root growth of ginseng. Lime-bordeaux caused damage on leaf when it was sprayed on ginseng between April and early May. No difference was found in root growth by spraying lime-bordeaux mixture between ratio 4-4 and 8-8 ratio in concentration. Plot of 6-6 raito and 8-8 ratio appeared to be similar efficacy compared to that of practical chemical control. However, the plot of 4-4 ratio showed lower than that of chemical control.
Characteristics of Growth and Yield by Planting Density and Mulching Materials in Salvia miltiorrhiza Bunge
Kim, Young Guk ; Yeo, Jun Hwan ; Han, Sin Hee ; Hur, Mok ; Lee, Young Seob ; Park, Chung Berm ;
Korean Journal of Medicinal Crop Science, volume 21, issue 3, 2013, Pages 179~183
DOI : 10.7783/KJMCS.2013.21.3.179
This study was carried out to investigate the variation of growth and yield using different planting densities and vinyl mulching in S. miltiorrhiza cultivation. Top plant growth was observed in the
planting density of S. miltiorrhiza;, plant height, leaves and branches were larger than in the other treatments. Root length, root diameter, and supporting roots of underground part were increased as planting density was wide, and dry root weight was increased in
. However, yield was highest at 294 kg/10a in the planting density of
. The use of 30-31cm white and black vinyl mulching had no significant effect on the plant height of S. miltiorrhiza, as compared to non-mulching. However, the highest volume of leaves and plant weight was observed in plants using 24.7 black vinyl mulching. Dry underground root weight was highest in black vinyl mulching at 21.7 g compared to 17.0 g for non-mulching. Yield per 10a increased by 28% using black vinyl mulching compared to non-mulching. Based on the results of this study, planting density of
and black vinyl mulching are the most suitable in the cultivation of S. miltiorrhiza.
Comparison of Ginsenoside Content According to Age and Diameter in Panax ginseng C. A. Meyer Cultivated by Direct Seeding
Han, Jin Soo ; Tak, Hyun Seong ; Lee, Gang Seon ; Kim, Jung Sun ; Choi, Jae Eul ;
Korean Journal of Medicinal Crop Science, volume 21, issue 3, 2013, Pages 184~190
DOI : 10.7783/KJMCS.2013.21.3.184
This study was carried out to investigate ginsenoside content in different root parts and the correlation between root diameter and ginsenoside composition of Panax ginseng C. A. Meyer cultivated by direct seeding. The unit contents of ginsenoside were 29.65, 28.76, 26.34 mg/g, respectively in 4, 5, 6 years old. However, the total contents of ginsenoside were 431.97, 606.56, 657.80 mg/root, respectively. Total ginsenoside content of fine root was higher than that of main root and lateral root. These tendencies were related to decrease by the increase of root diameter. When diameter of main root and lateral root were the same in different ages, the total ginsenoside content was higher in the order of 4 > 5 > 6 years old roots. Except for ginsenoside-Rg1, other ginsenosides components (PD/PT and total ginsenosides) had highly negative correlation with the root diameter within whole root, main root, lateral root and fine root, which indicated that ginsenoside content is correlated to root diameter. As results, it is suggested that ginsenoside content can be predicted.
Effect of Five Korean Native Taraxacum on Antioxidant Activity and Nitric Oxide Production Inhibitory Activity
Choi, Kyeong Hee ; Nam, Hyeon Hwa ; Choo, Byung Kil ;
Korean Journal of Medicinal Crop Science, volume 21, issue 3, 2013, Pages 191~196
DOI : 10.7783/KJMCS.2013.21.3.191
The effect on the antioxidant activity and Nitric Oxide activity production inhibitory activity of Taraxacum has not been known. Therefore, phenolics and flavonoid contents were investigated from the ethanol extracts of five different Taraxacum species. The results showed that, among the five Taraxacum, T. hallaisanensis contains the highest total phenolic and flavonoid contents. When the antioxidant activity was measured by DPPH,
and reducing power activity, the free radical scavenging activity of T. hallaisanensis was also the highest among five Taraxacum species. However, measurement by CCK-8 assay in Raw264.7 cells indicated that the extracts of Taraxacum species have no effect on cell viability. Moreover, we also investigated the effect of Taraxacum species on NO scavenging activity in lipopolysaccharide (LPS)-stimulated Raw264.7 cells. The results clearly showed that Taraxacum species inhibited NO production, and the inhibitory effect of T. hallaisanensis was the strongest. The above results suggested that Taraxacum species affected the antioxidant and NO scavenging activity, and among the five species, antioxidant and NO scavenging activity assay of T. hallaisanensis was significantly higher than those of other four Taraxacum species. Therefore, T. hallaisanensis could be used as a potential drug with anti-oxidant and anti-inflammatory effect.
Antioxidant Activities and α-Glucosidase Inhibitory Effect of Water Extracts from Medicinal Plants
Kim, Hyun Sook ; Kim, Tae Woo ; Kim, Dae Jung ; Lee, Jae Sung ; Kim, Kyoung Kon ; Choe, Myeon ;
Korean Journal of Medicinal Crop Science, volume 21, issue 3, 2013, Pages 197~203
DOI : 10.7783/KJMCS.2013.21.3.197
We studied the total polyphenol content, DPPH radical scavenging activity, hydroxyl radical scavenging activity and
-glucosidase inhibition of water extracts from 17 medicinal plants. Total polyphenol contents ranged from 10.0 (Coix lachryma-jobi L, CL) ~ 279.7 (Perilla sikokiana, PS)mg/g. The water extract from medicinal plants were evaluated for its free radical scavenging activities and compared with a commercial antioxidant, ascorbic acid. DPPH radical scavenging activity of Pyrus pyrifolia (PP), Chamaecyparis obtusa L. (COL), Chamaecyparis obtusa F. (COF), and PS were higher than positive control. Higher hydroxyl radical scavenging activity were shown in Acanthopanax senticosus (AS) and Cordyceps militaris (CM) than the other plants. The highest anti-
-glucosidase activity was observed in Cornus officinalis (CO) and Paeonia suffruticosa Andrews (PSA) water extracts. PSA showed not only the higher DPPH radical scavenging activity but also the anti-
-glucosidase activity. The results of our study that PP, COL, COF, PS, AS, CM, CO and PSA could be potential candidates for natural antioxidants.
Enhancement of Cosmeceutical Activity from Codonopsis lanceolata Extracts by Stepwise Steaming Process
Kim, Ji Seon ; Choi, Woo Seok ; Chung, Jae Youn ; Chung, Hee Chul ; Lee, Hyeon Yong ;
Korean Journal of Medicinal Crop Science, volume 21, issue 3, 2013, Pages 204~212
DOI : 10.7783/KJMCS.2013.21.3.204
In general, stepwise hot steaming process is known to be effective in improving its biological activities; however, not much employed in processing Codonopsis lanceolata due to its hardness. The complex processed C. lanceolata showed highest free radical scavenging acitivity as 45.21%. Total phenol and flavonoid content were of complex processed C. lanceolata higher than conventional extract and alone steaming process. It was showed the lower melanogenesis rate on melanin production test by B16F10 cells as 27.46%. High inhibitory of tyrosinase was also measured as 28.61% by adding steamed Codonopsis lanceolata extracts by high pressure extraction of 1.0
. And anti-wrinkle activity were 39.08%. In comparing phenolic acids profiles in the extract, in general higher amounts of polyphenol were obtained possibly by easy release of active components during thermal processing, which results in better antioxidant activities than that of general extract. This findings can also be supported by result that the extract by steaming process showed better activities than the general extraction extract.
Preparation Method of Indigo Standard Solution and Variation of Indigo Contents in Blue Dye Extract from Breeding Lines of Persicaria tinctoria H. Gross
Kim, Seong Ju ; Ko, Jae Hyung ; Park, Si Hyung ; Kim, Myeong Seok ; Kim, Kwan Su ;
Korean Journal of Medicinal Crop Science, volume 21, issue 3, 2013, Pages 213~219
DOI : 10.7783/KJMCS.2013.21.3.213
This study was carried out to find out the optimum method of preparation of indigo standard solution and its stability, and to investigate the indigo contents in Niram, blue dye extract, from a total of 7 indigo plants and 34 breeding lines of Persicaria tinctoria H. Gross. Proper solvent for indigo standard was dimethyl sulfoxide (DMSO), and appropriate concentration was 1 mg of indigo in 10 mL of DMSO. Absorbance value of UV/Vis Spectrophotometer at 620 nm of standard solution was changed decreasingly 12 hours after the preparation of standard solution irrespective of the storage conditions such as temperature and light. Average value of absorbance of 8-fold diluted standard solutions prepared daily during 16 days was
, indicating the powder of indigo compound was stable chemically. Calibration curve was made for quantitative analysis of indigo of 7 Niram samples, and indigo contents ranged from 0.69% to 18.76% showing relatively larger variation. Across all 34 breeding lines, the range of indigo content was from 7.9 mg to 56.4 mg per 100 g of fresh leaves, averaging 25.2 mg of indigo content and showing a 47.7% coefficient of variation.
Comparison of Genetic Diversity among Amaranth Accessions from South and Southeast Asia using SSR Markers
Wang, Xiao Qiang ; Park, Yong Jin ;
Korean Journal of Medicinal Crop Science, volume 21, issue 3, 2013, Pages 220~228
DOI : 10.7783/KJMCS.2013.21.3.220
This study was conducted to assess the genetic diversity and population structure of 70 amaranth accessions collected from South and Southeast Asia using 14 simple sequence repeat (SSR) markers. In total, 67 alleles were detected, with an average of 4.79 per locus. Rare alleles comprised a large portion (46.3%) of the detected alleles, and 29 unique alleles associated with rice accessions were also discovered. The mean major allele frequency (MAF), genetic diversity (GD) and polymorphic information content (PIC) of the 14 SSR loci were 0.77, 0.36, and 0.34, respectively. A model-based structural analysis revealed the presence of three subpopulations. The genetic relationships revealed by the neighbor-joining tree method were fairly consistent with the structure-based membership assignments for most of the accessions. All 70 accessions showed a clear relationship to each cluster without any admixtures. We observed a relatively low extent of genetic exchange within or among amaranth species from South and Southeast Asia. The genetic diversity results could be used to identify amaranth germplasms and so facilitate their use for crop improvement.