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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Medicinal Crop Science
Journal Basic Information
Journal DOI :
The Korean Society of Medicinal Crop Science
Editor in Chief :
Volume & Issues
Volume 7, Issue 4 - Dec 1999
Volume 7, Issue 3 - Sep 1999
Volume 7, Issue 2 - Jun 1999
Volume 7, Issue 1 - Mar 1999
Selecting the target year
Effects of Carbon, Nitrogen Sources and pH on Direct Somatic Embryogenesis in Liquid Culture of Rehmannia glutinosa Lib.
Chae, Young-Am ; Park, Ju-Hyun ;
Korean Journal of Medicinal Crop Science, volume 7, issue 1, 1999, Pages 1~6
Basic informations for direct somatic embryo formation in Rehmannja glutinosa Lib. were obtained in 500ml erlenmyer flask. The ratio of ammonium to nitrate nitrogen of 825(mg/l) : 1900(mg/l) was proper condition for somatic embryo formation from stem and petiole explants and 3% sucrose was the most effective carbon source. Full strength MS medium with 2mg/l BA was better than LS medium for somatic embryogenesis. The initial pH 5.7 of medium(full strength MS with 2.0mg/l BA and 0.1mg/l NAA) was good for embryo production. Potassium ion was taken up rapidly within 2 weeks. while
ion contents were almost constant during culture period. Sucrose hydrolysis occurred throughout the culture, while glucose and fructose were absorbed simultaneously from the third week of culture.
Effects of Storage Temperatures and Periods on the Direct Somatic Embryogenesis in Rehmannia glutinosa Liboschitz and Scrophularia buergeriana Miquel
Park, Ju-Hyun ; Song, Ji-Sook ; Ohk, Hyun-Choong ; Lim, Wan-Sang ; Chae, Young-Am ;
Korean Journal of Medicinal Crop Science, volume 7, issue 1, 1999, Pages 7~10
The effects of storage temperature and periods as pretreatments on direct somatic embryogenesis or shoot development in liquid culture of leaves, stems and petioles of Rehmannia glutinosa and Scrophularia buergeriana were investigated. Proper storage Temperature for Rehmannia glutinosa was
and the leaf stock could be stored up to 12 weeks. In Scrophularia buergerjana. shoots were developed from the 20 week-stored petiole and stem at
. In general,
was considered the most effective storage temperature in terms of storage cost.
Germanium(IV) Content in the Korean Angelica keiskei Koidz
Jeong, Seung-Il ; Han, Wan-Soo ;
Korean Journal of Medicinal Crop Science, volume 7, issue 1, 1999, Pages 11~15
The uptake of germanium(Ge) by health foodstuffs was interesting because of a therapeutic effect of organic germanium. Germanium contents in different plant parts of the cultivated Korean Angelica keiskej Koidz in several growing districts were determined by square wave anodic stripping voltammetry. Experimental conditions in germanium determination from Angelica kejskej Koidz were as follows : deposition time; 20 sec, deposition potential; -0.9 volts vs Ag/AgCl, and frequency; 100 Hz in
, supporting electrolyte solution at pH 2.43 containing
pyrocatechol violet. Calibration curve showed a good lineality in the range of 0.4 ppb to 2.0 ppm and the detection limit was 0.08 ppb. There was a large difference in content according to growing districts ranging from 102 to 386ppm. Germanium content in leaf parts was much higher than that in stems.
Effect of Gibberellin and Chilling Treatments on Breaking Epicotyl Dormancy and Seedling Growth of Polygonatum odoratum Druce
Kang, Jin-Ho ; Kim, Dong-Il ; Bae, Kee-Soo ; Kim, Seok-Hyeon ; Chung, Jong-Il ;
Korean Journal of Medicinal Crop Science, volume 7, issue 1, 1999, Pages 16~21
Seed-propagation of Solomon's seal (Polygonatum odoratum) takes 2 years to shorten the period for becoming a practical method. The experiment was done to establish a proper method of breaking the epicotyl dormancy in bulk seed-propagation. Seedlings with a bulbil were treated with
every 2 days for 4 or 8 days and chilling treatments at
were enforced for 4, 6, 8 or 12 weeks. Emergence- and growth-related characteristics were examined immediately after the treatments, 3 and 6 weeks later. Rate of cotyledonary sheath rupture immediately after
treatment was greater in its 8-day treatment than in 4-day although its effect disappeared later. However, any epicotyl treated with
solution did not elongate so that new seedlings disemerged over the bed soil. That resulted from not breaking the epicotyl dormancy since
did not rupture all of the cotyledonary sheath formed with several sheets and consequently, the solution did not reach it. The
treatment for bulk seed-propagation, therefore, was impractical method. On the contrary, the chilling treatment was able to be applied to the seed-propagation because of getting the cotyledonary sheath rupture and the epicotyl elongation. Seedling emergence and its growth after chilling treatment were influenced by chilling period although required at least over 6-week treatment for satisfactory results.
Growth Characteristics of Acanthopanax sessiliflorus Seem Seedling Grown under Different Conditions and Seasons
Cho, Seon-Haeng ;
Korean Journal of Medicinal Crop Science, volume 7, issue 1, 1999, Pages 22~26
This study was investigated to elucidate the growth characteristics of Acanthopanax sessiliflorus grown under different conditions and seasons. The emergence percent of seeds sown in the vinyl house was about 2.2 times better than that in the open field. The plants which had 2 to 3 normal leaves showed the highest value in root setting percent, and the root setting percent was decreased significantly in plants with one normal leaf and 4 to 5 normal leaves. The root setting percent of plants transplanted from April 19 to May 2 was 100%, but plants transplanted at April 4 or from May 17 to June 1 showed lower root setting. Tree percent, plant height. root length and fresh weight of plants grown by transplanting culture were better than those of plants grown by direct seeding.
Effect of Mechanical Peeling Time on Yield and Quality of Paeonia lactiflora Pallas Root
Kim, Ki-Jae ; Park, Chun-Hong ; Shin, Jong-Hee ; Kim, Se-Jong ; Park, So-Deuk ; Choe, Boo-Sull ;
Korean Journal of Medicinal Crop Science, volume 7, issue 1, 1999, Pages 27~30
This experiment was carried out to determine the adequate machine peeling and retting time of peony roots, which are used as medicinal resources in Korea. When the roots were peeled for
minitues in the peeling machine, the epidermis of root was removed, and higher yield was obtained and paeoniflorin content as compared with roots which were peeled for longer time. The paeoniflorin content of root varied with the different parts of root. The highest content was recorded in the upper part and the lowest in the middle part. The loss of paeoniflorin increased in the roots processed for expanded peeling and retting time. Lightness of peeled root was higher and
was lower in the roots which were peeled by machine for longer time.
Effects of Sulfuric Fertilizers on Growth and Allylisothiocyanate Contents of Wasabia japonica
Cultivated in Heating Condition
Moon, Jung-Sub ; Song, Young-Ju ; Ko, Bok-Rae ; Kim, Dong-Won ; Sung, Moon-Ho ;
Korean Journal of Medicinal Crop Science, volume 7, issue 1, 1999, Pages 31~36
This study was carried out to investigate the effect of sulfuric fertilizers on allylisothiocyanate(allylNCS) content of upland Wasabia japonica Matsum cultivated in heating condition in the winter season. The rhizome's growth characters following soil application of sulfur dust were not significantly different compared to control, though its soil acidity and available sulfur content was higher than other treatments. Total sulfur content of plant was increased by soil application as compared to foliar application and allylNCS content of rhizome increased up to 22.8% in the sulfur dust treatment. The result showed that soil application was effective for supplying
in upland Wasabia japonica Matsum when cultivated in heating condition. In the analysis of correlation coefficients between growth and quality characteristics, allylNCS content of rhizome showed the significantly positive correlation with total sulfur content of shoot, rhizome and allylNCS content of shoot.
Screening of Korean Medicinal and Food Plants with Antitumoral Activity
Chung, Ill-Min ; Kim, Kwang-Ho ; Ahn, Joung-Kuk ; Ahn, Jong-Seog ; Ahn, Soon-Cheol ;
Korean Journal of Medicinal Crop Science, volume 7, issue 1, 1999, Pages 37~44
This study was conducted to screen antitumoral activity by in vitro bioassay method using 60 Korean medicinal and food plants extracted by 80% EtOH. Antitumor activity test was applied by the PKC(protein kinase C) and antibleb formation, PLC (Phospholipase C), and colorimetric tetrazolium assay (MTT assay) methods. Chenopodjum album and black Glycine max showed high antitumoral activity by 73.5% and 81.0%, respectively, against PKC by bleb-forming assay and PKC enzyme assay on human chronic leukemia K562 cell. Black Glycine max also showed 91.2% antitumoral activity in the PLC method and the lowest
by MTT method against P-338 cell line. In the effect of the concentration treatment on antitumoral test, the more concentration indicated the more activity value.
Isolation and HPLC Analysis of Timosaponin A III from Rhizomes of Anemarrhena asphodeloides
Kim, Geum-Soog ; Park, Chang-Kie ; Seong, Jae-Duck ; Kim, Hyun-Tae ; Han, Sang-Ik ; Kwack, Yong-Ho ;
Korean Journal of Medicinal Crop Science, volume 7, issue 1, 1999, Pages 45~50
Timosaponin A III, an active and major compound, was isolated from rhizomes of Anemarrhena asphodeloides. The quantitative analysis of timosaponin AIII was performed by a high performance liquid chromatographic(HPLC) method using ELSD and the useful extraction method for HPLC analysis was examined as well. This HPLC method can be utilized as the standard analytical method for the evaluation of the quality of Anemarrhena rhizoma in the steps of breeding and cultivation. Additionally, the HPLC analysis method can be useful for the evaluation of the quality of Anemarrhena rhizoma sold as a traditional medicine in current markets.
Antioxidative Compounds in Extracts of Acer ginnala Max.
Han, Seong-Soo ; Lo, Seog-Cho ; Choi, Yong-Hwa ; Kim, Myong-Jo ; Kwak, Sang-Soo ;
Korean Journal of Medicinal Crop Science, volume 7, issue 1, 1999, Pages 51~57
To search for antioxidative compounds from plant resources, methanol extracts of 45 plant species were investigated using DPPH method. The highest activity was shown in the methanol extract of Acer ginnala(
), followed by Stewartia koreana(
) and Carpinus laxiflora(
). Two antioxidative compounds were isolated from the methanolic extract of Acer ginnala Max and identified as acertannin(2, 6-di-O-galloyl-1, 5-anhydro-D-glucitol) and gallicin (methyl-3, 4, 5-trihydroxybenzoic acid) on the basis of mass spectroscopy,
data. The DPPH free radical scavenging activities of acertannin(
) and gallicin(
) were more effective than those of BHA(
Aromatic Constituents and Essential Oil Content of Codonopsis lanceolata Trautv Cultivated at Different Altitudes
Kim, Sang-Kuk ; Kang, Dong-Kyoon ; Min, Gi-Gun ; Chung, Sang-Hwan ; Lee, Seong-Phil ; Lee, Sang-Chul ; Choi, Boo-Sull ;
Korean Journal of Medicinal Crop Science, volume 7, issue 1, 1999, Pages 58~62
This study was to determine aromatic constituents and essential oil content in the root of Codonopsis lanceolata grown at different altitudes; Andong(150m), Mt. Hagka(550m) and Mt. Irwol(750m). Soil pH in Mt. Hagka and Mt. Irwol was 5.6 and 6.1, respectively, which was lower than in Andong(150m), but organic matter content was higher than that of Andong with 6.7 to 8.8%. Fresh root yield also increased as cultural region was located at higher altitude. In particular, yield in Mt. Irwol was 281kg/10a. Essential oil content was highest of 0.006% in the Mt. Irwo1. Forty-eight volatile aromatic compounds in the root of Codonopsis lanceolata were identified by GC/MS. Major aromatic compounds were 1-hexanol, cis-3-hexano1. and Trans-2-hexanol. In partucular, Trans-2-hexanol, was highest in Mt. Irwol attaining the 47.22% area. As a result, it was considered that cultural region above 700m in altitude was the most effective for the improvement of essential oil and aromatic constituents in the roots of Condonopsis lanceolata Trautv.