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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Medicinal Crop Science
Journal Basic Information
Journal DOI :
The Korean Society of Medicinal Crop Science
Editor in Chief :
Volume & Issues
Volume 7, Issue 4 - Dec 1999
Volume 7, Issue 3 - Sep 1999
Volume 7, Issue 2 - Jun 1999
Volume 7, Issue 1 - Mar 1999
Selecting the target year
In vitro selection and plant regeneration from fusaric acid-tolerant Cell Lines of Rehmannia glutinosa Lib.
Yu, Chang-Yeon ; Jin, Zheng-Lu ; Jeong, Jae-Young ; Lim, Jeong-Dae ; Chae, Young-Am ;
Korean Journal of Medicinal Crop Science, volume 7, issue 4, 1999, Pages 245~250
Callus growth of fusaric aicd-tolerance cell lines was different depending on fusaric acid concentrations. But callus growth on medium with fusaric acid was higher than that on medium without fusaric acid. Especially, RF-9, RF-11 and RF-15 showed high callus growth at
fusaric acid. After subculturing on medium without fusaric acid for 5 weeks, fusaric acid -tolerant stability was investigated. Cell lines at
fusaric acid were showed over 60% callus growth, callus growth rate at
fusaric acid was decreased until 30-80% of control. Regeneration capacity of fusaric acid-tolerant cell lines was different depending on fusaric acid concentrations. Thirteen cell lines regenerated the shoot over at
fusaric acid, and only two cell lines were not regenerated.
The Effect of Various Culture Media on Histological Anatomy and Saikosaponin Content in Hairy Root Culture of Buplurum falcatum
Ahn, Jun-Cheul ; Jeong, Young-Jae ; Lee, Kyu-Bae ; Kim, Ock-Tae ; Hwang, Baik ;
Korean Journal of Medicinal Crop Science, volume 7, issue 4, 1999, Pages 251~256
Growth rate and saikosaponin content of Buplurum falcatum hairy root on MS and RCM basal media were measured. And using hairy root cultured in those media, it was investigated whether some correlation between differences in growth rate and saikosaponin content and histiological difference was or not. Results obtained showed that among media tested, MS and 3RCM medium which showed the faster growth in fresh weight and dry weight, respectively. And saikosaponin content of hairy root cultured in 3RCM was
times higher than that of 1 year cultivated roots, whereas that in MS was extremely low. On the other hand, histological differences of root tips and about 1 cm region from root tip cultured in both MS and 3RCM were not prominent, however, in mature region, a lot of cells only in 3RCM contained densely stained vacuoles or organelles which probably contain tannin, suggesting that development of those has a correlation with biosynthesis of secondary metabolites containing saikosaponin.
Comparison of Biological Activities on Rehmannia Radix and R. Radix Preparata produced in Korea
Ahn, Sang-Wook ; Kim, Young-Gil ; Kim, Min-Hae ; Lee, Hyeon-Yong ; Seong, Nak-Sul ;
Korean Journal of Medicinal Crop Science, volume 7, issue 4, 1999, Pages 257~262
We investigated the biological activities on ethanol extracts of R. glutinosa and R. glutinosa Preparata. The result of anti-mutagenicity suggested that the ethanol extract of R. glutinosa Preparata showed stronger activity than that of R. glutinosa ethanol extract. All of the ethanol extracts showed over 50% growth inhibition of several cancer cell lines. Especially, 61% of the cell growth of Hep3B was inhibited by adding 1g/ l of ethanol extract of R. glutinosa Preparata. In hypoglycemic activities and controlling blood pressure, 63.69% of
and 56.58% of ACE activities were inhibited by adding 1g/ l of ethanol extract of R. glutinosa Preparata. In general, the ethanol extract of R. glutinosa Preparata showed higher biological activities than those of R. glutinosa.
Comparison of Hepatic Detoxification activity and reducing Serum Alcohol concentration of Hovenia dulsis
and Alnus japonica Steud.
An, Sang-Wook ; Kim, Young-Gil ; Kim, Min-Hae ; Lee, Byung-Ik ; Lee, Sang-Ho ; Kwon, Hyuk-Il ; Hwang, Baik ; Lee, Hyeon-Yong ;
Korean Journal of Medicinal Crop Science, volume 7, issue 4, 1999, Pages 263~268
It was found that the level of alcohol concentration in both mouse and human sera can be significantly decreased up to 42% by oral administration of the mixtures of the extracts of Hovenia dulcis THUNB and Alnus japonica Steud. A single treatment of extract from Hovenia dulcis reduced the serum alcohol concentration to 32%, compared to 13% in treating the extract of Alnus japonica. Similar patterns were observed in enhancing alcohol dehydrogenase (ADH) and glutathion-S-transferase (GST) activity in the liver. The inhibition of cathepsin activity was also greatly reduced by administrating the mixture of both extracts : however, the extract of Alnus japonica did not affect the acitivity of cathepsin. It was concluded that the mixture of both extracts had synergic effect on reducing serum alcohol concentration and improving the detoxification process due to alcohol administration in the liver.
Growth and Seed Yield of Safflower in Plastic house
Kim, Ki-Jae ; Shin, Jong-Hee ; Park, Chun-Hong ; Park, So-Deuk ; Choi, Boo-Sull ;
Korean Journal of Medicinal Crop Science, volume 7, issue 4, 1999, Pages 269~274
This experiment was carried out to investigate the effect of rain shelter cultivation on the its quality of safflower. The development and growth of safflower, especially the number of secondary branches per plant was increased by rain shelter. Under the rain shelter started earlier, the seed yield was increased by 95% compared with that of non-shelter condition. The number of plants in a unit area the number of flower buds and seeds per plant and seed weight were increased in rain shelter condition. As those plants were cultivated under the rain shelter, lightness(L) of their seeds was increased but redness (a) and yellowness (b) were decreased.
Plant Regeneration and Saponin Contents in Codonopsis lanceolata L.
Choi, Myung-Suk ; Choi, Pil-Son ;
Korean Journal of Medicinal Crop Science, volume 7, issue 4, 1999, Pages 275~281
Embryogenic calli of Codonopsis lanceolata were cultured on MS agar medium containing various concentrations of sucrose as a carbon source. Upon transfer to MS basal medium, somatic embryos of cotyledonary stage converted to plantlets. When sucrose was added with greater than 4%, the number of shoots and roots regenerated from somatic embryo increased. However, the growth of shoots and roots was retarded in agar medium with more than 2% sucrose, but promoted in medium with lower concentration of sucrose. Saponin contents of shoots regenerated from somatic embryos, embryogenic calli, non-embryogenic calli, and native roots were determined by HPLC. Saponin contents of native root was variable, depending on regenerant, embryogenic calli, and cotyledonary embryos. The saponin contents of regenerated roots in medium with high sucrose was similar to native roots. Saponins content based on cell differentiation to shoot and root was dramatically decreased. This results could be effectively controlled for the production of useful secondary metabolites.
Root Yield and Saponin content in Different soil Texture of Platycodon grandiflorum A.DC.
Seong, Jae-Duck ; Kim, Hyun-Tae ; Kim, Geum-Soog ; Han, Sang-Ik ; Kwack, Yong-Ho ;
Korean Journal of Medicinal Crop Science, volume 7, issue 4, 1999, Pages 282~287
This study was conducted to investigate the effects of different soil texture in agronomic characteristics, root yield and quality of baloon flower (Platycodon grandiflorum A. DC.). Three kinds of soil texture, sandy loam, silt loam and clay loam, were tested for two years. Baloon flowers were seeded with 20cm row space and cultivated in the one square meter pot with 0. 6m depth. Fifty plants per pot were remained. In growth characteristics of one-year-old plants, shooting date was not significantly different according to the changes of soil texture. The ratio of stem growth was highest between July and August. In two-years-old plants, it was showed between May and June. Plants grown on clay loam showed the highest growing characteristics than those of other soil textures. Dry root weight cultivated on clay loam was 274kg/10a in one-year-old and 934kg/10a in two-years-old plants. The content of ethanol extracts from root was highest at those of sandy loam as 47.1 % in one-year-old root. The crude saponin content was not different significantly among the three kinds of soil texture.
Growth and Yield in different Hydroponic Solutions of Angelica keiskei Koidzumi
Han, Seoung-Ho ; Choi, Byung-Jun ; Han, Kwang-Seop ; Shin, Cheol-Woo ; Moon, Chang-Sik ; Seo, Sang-Duck ; Chang, Ki-Woon ; Park, Sang-Il ;
Korean Journal of Medicinal Crop Science, volume 7, issue 4, 1999, Pages 288~295
This experiment was conducted to evaluate the appropriate nutrient solution for hydroponics in Angelica keiskei. The nutrient solutions used were Korean solution, Yamazaki solution, Netherlands solution and open field cultivation. The highest yield of Angelica keiskei was 4, 542kg/10a at Netherlands solution. 'The yield at Netherlands solution was increased about 139 percent, compared to that at open field cultivation. The highest germanium contents was 0. 35ppm at Korean solution. The contents increased 40 percent at Korean solution compare to that at open field cultivation.
Enrichment on the Differentiation of Multi-shoots and Saponin contents in Tissue culture of Korean ginseng (Panax ginseng C. A. Meyer)
Chung, Chan-Moon ; Bae, Kil-Kwan ;
Korean Journal of Medicinal Crop Science, volume 7, issue 4, 1999, Pages 296~302
This experiment was conducted to study the effect of
(0, 2, 500, 5, 000, 10, 000ppm) enrichment by enabling ventilation on micropropagation of multi-shoot and on the saponin contents in vitro in Korean ginseng (Panax ginseng C. A. Meyer). Embryo was cultured in Murashige and Skoog medium added 3mg/ l of Indolbutyric acid, Benzyladenin and Gibberellic acid
, enrichment had little effects on the number of adventitious buds and shoots originated from adventitious buds. The ratio of differentiated shoots to adventitious buds were about 50% in
, enrichment treatment. The shoots originated from adventitious bud showed more rapid growth and had larger leaf area than the shoots originated from the leaf primordia did. The number of shoot primordia was the highest in 2, 500ppm of
enrichment treatment. On the contrary, 10,000ppm of
, enrichment made smaller the number of shoot primordia and ratio of shoots to shoot primordia. The range of shoots differentiated was from shoot primordia were 15. 4 to 23. 9. The rate of dry weight of cultured shoots showed lowest (7. 5%) in control and highest (8. 59%) in 2, 500ppm of
, enrichment. Rate of in vitro flower in control was 7.6% and that in 2500ppm of
was about twice (15.7-16.3%) as much as in control. Flower number per a embryo cultured was about 1.2-1.3. In the multi-shoots with callus enriched by 2, 500ppm of
, the contents of crude saponin and ginsenosides in multi-shoots alone were higher than in multi-shoots with callus. The characteristics of ginsenosides in multi-shoots were especially the higher content of ginsenoside Rd, Re, and
Purification and Identification of Antioxidative Components from the Fruits in Pyrus ussuriensis Maximowicz
Kim, Myong-Jo ; Rim, Yo-Sup ; Song, Won-Seob ; Kim, E-Hun ; Yu, Chang-Yeon ;
Korean Journal of Medicinal Crop Science, volume 7, issue 4, 1999, Pages 303~307
Three antioxidative compounds were isolated from the EtOAc extract of the fruits of Pyrus ussuriensis. On the basis of spectroscopic evidences, the structures of these compounds were established as 1, 4-dibenzenediol, chlorogenic acid and quercitrin. The DPPH free radical scavenging activities of 1, 4-dibenzenediol
and chlorogenic acid
were more effective than those of BHA
Farm Study of Direct Seeding Cultivation of the Korean Ginseng(Panax ginseng C. A. Meyer)
Won, Jun-Yeon ; Jo, Jae-Seong ;
Korean Journal of Medicinal Crop Science, volume 7, issue 4, 1999, Pages 308~313
Direct seeding is a cultivation method of Korean ginseng which can cut down production cost and increase productivity. This study was conducted to obtain detailed information about this method and to aid the development of it in Korea. Major pre-crops before ginseng cultivation were rice, corn and rye, and organic matters, such as rice straw, rye straw and poultry feces were used for basal fertilization. In direct seeding of the ginseng field, seeding density was 196 to 210 seeds per
and germination ratio was 67%. Survival ratio of 4-year-old ginsengs which were products of direct seeding was 51%, and more than 100 plants per
survived. The most critical diseases in the directly seeded ginseng field were grey mold, damping off, and stem diseases including stem spot disease. Plant growth of the ginseng cultivated by the direct seeding method was not different from the ginseng cultivated by transplanting method. But the root of the ginseng cultivated by the direct seeding method took the shape of a shorter main root compared to the ginseng cultivated by the transplanting method. Root yield per
of the direct seeding ginseng field was larger compared to the field of the transplanting cultivation.
New Cultivar ‘Yeonjeong’ of Medicinal Forsythia
Kim, Jae-Chul ; Park, So-Deuk ; Kim, Jeong-Hye ; Park, Jun-Hong ; Kim, Se-Jong ; Kim, Ki-Jae ; Ryu, Jung-Ki ; Choi, Boo-Sull ; Kim, Tak ;
Korean Journal of Medicinal Crop Science, volume 7, issue 4, 1999, Pages 314~315
Industrial Development of Medicinal Plants in China
Gao, Wen-Yuan ; Paek, Kee-Yoeup ; Peng, Yong ; Xiao, Pei-Gen ;
Korean Journal of Medicinal Crop Science, volume 7, issue 4, 1999, Pages 316~324
In China, medicinal plants play almost the same important role in the general health service as the western medicine. In this paper, we present a brief review about the industrial development of medicinal plants in China. A nation-wide survey shows that there are 11, 118 species of medicinal plants in China. The total annual output value of natural products is about $6, 000. The output value of preparations is 3 billion US dollars. Over 100 new drugs have been developed from the medicinal plants. As the trend worldwide today that human-being favors the way of life back to nature, medicinal plants will play a very important role, and their industrial exploitation will certainly be keeping a key position for human health.