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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Journal of Medicinal Crop Science
Journal Basic Information
Journal DOI :
The Korean Society of Medicinal Crop Science
Editor in Chief :
Volume & Issues
Volume 8, Issue 4 - Dec 2000
Volume 8, Issue 3 - Sep 2000
Volume 8, Issue 2 - Jun 2000
Volume 8, Issue 1 - Mar 2000
Selecting the target year
Antioxidative compounds of Achillea sibirica Ledeb
Moon, Hyung-In ; Lyu, Sung-Hyo ; Roh, Jong-Hwa ; Zee, Ok-Pyo ;
Korean Journal of Medicinal Crop Science, volume 8, issue 1, 2000, Pages 1~8
Achillea sibirica Ledeb. is widely distributed in Korea and has been often used as folk medicine in peptic and tonic. As one of our searches for bioactive (anti oxidation) compounds from medicinal plants, we studied Achillea sibirica Ledeb. (Compositae). Antioxidant activity of Achillea sibirica was determined by measuring lipid peroxide produced when a mouse liver homogenate was exposed at
using 2-thiobarbituric acid (TBA) and by evaluation the radical scavenging activity on 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical. Whole parts of Achillea sibirica was extracted with methanol and its extracts was fractionated with organic solvent; n-Hexane, methylene chloride, ethyl acetate, n-Butanol. EtOAc fraction exhibited antioxidant activity and From its, two flavonoid glycosides were isolated by silica gel and gel filtration colume chromatography and identified to kampferol 3-O-glucoside and luteolin 7-O-neo-hesperidoside, respectively, by physico-chemical and spectroscopical method. At antioxidant activity test for two compounds isolated, antioxidant activity was showed too. And from hexane fraction sterol was is isolated and identificated to mixture of campesterol, stigmasterol, and
Effects of Carbon and Nitrogen Sources on the Shoot Formation in bioreator culture of Scrophularia buergeriana Miquel
Lim, Wan-Sang ; Chae, Young-Am ;
Korean Journal of Medicinal Crop Science, volume 8, issue 1, 2000, Pages 9~13
To determine the proper carbon and nitrogen sources and their proper levels for mass micro propagation of Scrophularia buergeriana Miquel, tonic and curing cough experiment were applied and a method for mass cultivation by using bioreactors (2.5 L) was expinined. Proper ratio of
was 413 mg/L : 1900 mg/L for multiple shoot production. Sucrose was more effective than glucose or fractose as carbon source and 3% concentration was good for shoot formation. Total nitrogen was not detected after six weeks both in 500 ml flask and bioreactor culture. Sucrose was decreased sharply after two weeks and there was no sucrose left after three weeks both in 500 ml flask and bioreactor culture. The stirrer in bioreactor caused shear stress to shoots severely. The sphere type bioreactor was better than the cylinder type and removal of inner loop in sphere type was more effective to avoid shear stress.
Enrichment on the Differentiation and Growth in tissue culture of Panax ginseng C. A. Meyer
Chung, Chan-Moon ; Bae, Kil-Kwan ; Aoki, Masatoshi ;
Korean Journal of Medicinal Crop Science, volume 8, issue 1, 2000, Pages 14~20
This experiment was conducted to investigate the effects of length of storage period under low temperature,
enrichment and addition of plant growth regulators in Murashige and Skoog medium on the plant regeneration of Korean ginseng (Panax ginseng C. A. Meyer). Seeds were treated for 60 and 80 days respectively under
environment. 2500ppm of
was enriched by ventilation. Three plant growth regulators added to the medium were Indolbutyric acid, Benzyladenin and Gibberellic acid (GA3). The result indicated that : The capacity of differentiation was higher in the aged cotyledons from the seeds treated for 80 days under low temperature condition than in those treated for 60 days.
enrichment had stimulating effects on the growth and development of shoot primordium significantly but less effects on the formation of adventitious buds. From one zygotic embryo hundreds of plantlets were differentiated.
enrichment had effects on the formation of both indirect somatic embryo and direct somatic embryo. Indirect somatic embryo showed little growth and differentiation, being undifferentiated vascular stele and epicotyl. Direct somatic embryos were formed on the epidermis of backside basal part of cotyledon. Those embryos developed to whole plant having latent bud.
Comparison of biological activity according to extracting solvents of four Acanthopanax root bark
Kim, Sung-Kyoung ; Kim, Young-Gil ; Lee, Mi-Kyoung ; Han, Jong-Soo ; Lee, Jin-Ha ; Lee, Hyeon-Yong ;
Korean Journal of Medicinal Crop Science, volume 8, issue 1, 2000, Pages 21~28
The biological activities of water, ethanol and 50% ethanol extracts from Acanthopanax root bark were compared. 94% of Hep3B cell growth was inhibited by adding 1.0g/L of 50% ethanol extracts from A. senticosus root bark. It was also showed that above 90% of A549 cell growth was inhibited by adding 1.0g/L of 50% ethanol extracts. The 50% ethanol extracts of A. sessiliflorum root bark showed that the extracts selectivity were from 1.5 to 3.4 by adding all samples. For screening immunomodulating activities, Jurkat(T-cell) was showed that the cell growth and viability were more increased and activited 275% by adding the 50% ethanol extracts from A. senticosus root bark. The result of anti-mutagenicity of 50% ethanol extracts of A. senticosus root bark was most effective than any other samples. The enhancement of glutathione-S-transferase activity was increased 241% by adding 1.0g/L 1 : 1 extracts of A. senticosus root bark. 72% of oxidation was inhibited by adding 1.0g/L of 50% ethanol extracts from A. senticosus root bark.
Effect of Different Media on Growth and Yield in Hydroponic culture of Angelica keiskei Koidzumi
Han, Seoung-Ho ; Choi, Byung-Jun ; Shin, Cheol-Woo ; Chang, Ki-Woon ; Han, Kwang-Seop ; Choi, Kang-Ju ; Woo, In-Sik ; Park, Sang-Il ;
Korean Journal of Medicinal Crop Science, volume 8, issue 1, 2000, Pages 29~40
This experiment was conducted to find appropriate media in hydroponic Culture of Angelica keiskei. The mediaused were carbonized rice hull, perlite, vermiculite, mixture(carbonized rice hull (1) : perlite (1) : peatmoss (1)), mixture+peatmoss20%, mixture+peatmoss40%, mixtrue+peatmoss60%, perlite 40%+peatmoss40%+vermiculite20% and open field cultivation. The highest yield of Angeli ca keiskei was shown 4,428ka/10a at vermiculite. The yield of vermiculite was increased about 133 percent compared to that of open field cultivation. The highest germanium contents was shown 0.52ppm at mixture+peatmoss 40%. The contents of germanium increased about 108 percent at mixture+peatmoss40% compared to that of open field cultivation.
Multiple Shoot Formation of Gentiana axillariflora Leveille by in Vitro Culture
Lim, Jung-Dae ; Yu, Chang-Yeon ;
Korean Journal of Medicinal Crop Science, volume 8, issue 1, 2000, Pages 41~48
This study was aimed to proliferate Gentiana axillariflora Leveille which is one of the important medicinal and ornamental plants, by establishment of multiple shoot formation and embryogenesis through tissue culture technique. Callus was formed on MS (Murashige and Skoog) medium supplemented with 2,4-D, CPA, but not formed with BAP. The addition of 2,4-D 2 mg/ l into the medium was effective for callus formation and the rate of callus formation was about 90%. Somatic embryos were obtained on MS medium for two months. When callus was cultured on MS medium with combination treatment of 2,4-D 0.5 mg/ l and BAP 0.5 mg/ l, the number of embryo formed was better than that of other single or combination treatments and the total numbers of embryo a were 18.8 (number of total embryo/number of explants incubated = 753/40) at mean. Callus induction from stem and node explants was increased by addition of TDZ 2 mg/ l in the presence of 2,4-D 2 mg/ l, respectively. The best result about the differentiation of shoots was obtained on MS medium added BAP 2 mg/ l from node culture. Multiple shoots from shoot apex were induced on MS medium containing BAP 1 mg/ I and TDZ 1 mg/ l , BAP 2 mg/ l and TDZ 1 mg/ l. The number of multiple shoots per one explant was above seventy plants. It was the most effective regeneration system for rapid multiplication of Gentiana axillariflora Leveille.
Changes in Volatile Compounds of Schizandra chinensis Fruits According to Drying and Extracting Methods
Kim, Kwan-Su ; Song, Ji-Sook ; Bang, Jin- Ki ;
Korean Journal of Medicinal Crop Science, volume 8, issue 1, 2000, Pages 49~57
To understand the effects of drying conditions on changes of volatile compounds in fruits of Schizandra chinensis, we analyzed SDE (steam distillation and extraction) extract and Headspace vapor of fresh and dried samples using GC/MS (Gas chromatograph/Mass spectrometer). Contents of essential oils from samples with different drying conditions were 0.58% in fresh ones, 0.60% in freeze dried ones, and 0.30% in hot-air dried ones. In SDE extract, major volatile compounds in fresh samples were terpinen-4-ol(9.01%),
, unidentified sesquiterpenes(28.48%), showing almost the same composition as that in freeze-dried ones, but those in hot-air dried samples at
, unidentified sesquiterpenes(50.38%), indicating the chemical changes during drying procedure. In Headspace vapor, major compounds in fresh samples were
, sabinene(8.48%), which were different from those in SDE extract. In chemical compositions of volatile compounds in dried samples,
decreased in the order of freeze-drying > hot-air drying at
> hot-air drying at
, camphene increased in the reverse order of the former. We observed the changes of the contents and compositions of essential oils compounds during drying procedure, especially a decrease in monoterpenes and alcohols and an increase in sesquiterpenes with relatively weak volatility.
Changes of Root Yield and Paeoniflorin Content affected by Harvesting Times in Peony(Paeonia lactiflora)
Kim, Ki-Jae ; Park, So-Deuk ; Park, Chun-Hong ; Shin, Jong-Hee ; Kim, Jae-Cheol ; Choi, Boo-Sull ;
Korean Journal of Medicinal Crop Science, volume 8, issue 1, 2000, Pages 58~63
Changes of yield and paeoniflorin content according to chemical control and harvest time were investigated. Peonies were harvested after four years from planting. Disease and pest control method was applied normally in first year. From second year to forth year we set two blocks and controlled them differently. In one, peonies were withered early under non-control condition. In the other which was controlled by spraying chemicals four time a year, top part withering was delayed. Harvest was taken four times top part withering was delayed. Harvest was taken four times: Jul. 25, Aug. 25, Sep. 25, and Oct. 25. In non-chemical, at harvest of Jul. 25, paeoniflorin content was highest and dry root weight was 1,126kg/10a which was little less than 1,177kg/10a of Aug. 25. In chemical control, dry root yield of Oct. 25. showed an increase of 33% over that of Jul. But paeoniflorin content in root at Oct. was lower than at Jul. or Aug. For high qualities and harvest yields of peony root, chemical control is necessary to maintain top part to late in Oct.
Effects of Seeding Date and Method on Growth and Yield in Dill (Anethum graveolens L.)
Han, Sang-Ik ; Sung, Jae-Deuk ; Kim, Hyun-Tae ; Kim, Geum-Soog ; Kwack, Yong-Ho ;
Korean Journal of Medicinal Crop Science, volume 8, issue 1, 2000, Pages 64~68
This study was conducted to determine effects of seeding date, method, and amount on the growth and yield in dill (Anethum graveolens L.). Dill was seeded at six different dates (from Feb. 15 to May 16 at 15 days interval) and two seeding methods (drilling and broadcasting). Required days to emergence, days to flowering and days to maturity were decreased as seeding date was late. In growth characteristics, plant height was significantly different at different seeding times, and lodging affect plant growth in the case of Feb. 15 and Mar. 2 seeding. Essential oil contents was not affected by different seeding times except Feb. 15. Seed yield was different at different seeding times. In seeding method tests, yield was increased by 14% in drill seeding compared with those in broadcast seeding. Regarding the amount of seeding, the yield from the plot of 3 l /10a was increased more 24% than 1 l/10a plot. As a result, this study suggest that dill have to be seeded before April 1 with drill seeding method and the optimum seeding amount appeared to be 3 l/10a.
Effects of Nitrogen Levels and Split Application Ratio on Growth and Yield in Liriope platyphylla WANG et TANG
Seong, Jae-Duck ; Park, Ki-Do ; Kwack, Yong-Ho ; Kim, Sung-Man ; Kang, Jin-Ho ;
Korean Journal of Medicinal Crop Science, volume 8, issue 1, 2000, Pages 69~73
To investigate proper method of nitrogen application, six levels of nitrogen fertilizer and five different split applications were tested in Liriope platyphylla. Leaf length and content of total nitrogen in plant increased with the increase of nitrogen application level from zero to 360kg per ha. Number and weight of tuber increased with the increase of nitrogen level and tuber production reached to highest level of 4.44M/T per ha with the level of 220kg nitrogen. And then, tuber production decreased with the addition of extra nitrogen fertilizer to level of 360kg per ha. In the research of proper nitrogen split application, tuber yield (3.52M/T per ha) was increased by 18% at the combination of 40% basal application and three times of top dressing, than that of 100% basal application.
Control System of Asian corn borer, Ostrinia furnacalis (Guenee) in Coix lacryma-jobi L. var. ma-yuen Stapf
Chang, Seog-Won ; Kim, Hee-Dong ; Kang, Chang-Sung ; Kim, Sung-Kee ;
Korean Journal of Medicinal Crop Science, volume 8, issue 1, 2000, Pages 74~78
This study was carried out to select the effective insecticides for control of Ostrinia furnacalis(Guenee) in Yonchon, northern part of Kyunggi province, where is main producing region of adlay in Korea. The Asian corn borer in adlay field had three generations a year. The first stage of adult activity was higher from late May to middle June, the second from late July to early August and the third late September. Based on difference in control value, the relative ranks of some insecticides were lambda cyhalothrin EC > cartap hydrochloride GR > carbaryl EC. In control value by different application timing of Lambda cyhalothrin EC, yields were significantly different, that is, twice applications on second generation showed the control value of
. Unhulled grain yield on Lambda cyhalothrin EC treated plot on second generation was higher than that in untreatred plot by 82%.
Effects of Seed Disinfectant Treatments on Leaf Blight and Smut Disease in Coix lacryma-jobi L. var. ma-yuen Stapf
Chang, Seog-Won ; Kim, Hee-Dong ; Jeon, Dae-Hoon ;
Korean Journal of Medicinal Crop Science, volume 8, issue 1, 2000, Pages 79~82
This study was carried out to investigate the effect of seed disinfectant on leaf blight and smut in adlay. Leaf blight and smut disease in adlay are known to be seed borne. Bjpolan's cojds, pathogen causing leaf blight in adlay, was detected from 32. 7 to 45. 7 % on seeds collected from adlay cultivated farms in Yonchon province. Periods of seed infestation are
days on the variety 'yulmu 1' and
days on 'Yonchon jaerae'. Distribution ratio of seed disinfestation periods was that no disinfestation,
days and more 11days were 20%, 20%, 45% and 15%, respectively. Frudioxonil W. S. treatment was effective on inhibition of leaf blight and smut disease when treated with infested seeds ; that is, disease control values of the fungicide are 97 and 93.4 percentage, repectively.