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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Medicinal Crop Science
Journal Basic Information
Journal DOI :
The Korean Society of Medicinal Crop Science
Editor in Chief :
Volume & Issues
Volume 9, Issue 4 - Dec 2001
Volume 9, Issue 3 - Sep 2001
Volume 9, Issue 2 - Jun 2001
Volume 9, Issue 1 - Mar 2001
Selecting the target year
Effects of Shading treatments and Harvesting methods on the Growth of Eleutherococcus senticosus Maxim
Han, Jong-Su ; Kim, Seung-Kyeong ; Kim, Sae-Won ; Kim, Young-Jin ;
Korean Journal of Medicinal Crop Science, volume 9, issue 1, 2001, Pages 1~7
This experiment was carried out to investigate the effects of shading treatments and harvesting methods on the growth of Eleutherococcus senticosus, which was known as the medicine of anticancer, anti-stress, hepatic detoxification activity, immunoactivity, and tonic. Light transmission ratio and air temperature were decreased as
, respectively, in 30% to 70% shading net treatment compared to non-shading. Fifty percentage shading net treatment was effective for the growth and yield of Eleutherococcus senticosus. During summer Eleutherococcus senticosus was growing under shading net treatment compared to non-shading. Tunnel type was more effective for growth than vertical type in shading method. 30cm length included 2 to 3 nodes cutting from soil surface was the most effective for branching stem length, plant height and yield as harvesting methods.
Cytotoxic Effects of Extracts from Hairy Roots of Rheum undulatum L.
Hwang, Sung-Jin ; Kim, Jae-Hun ; Ra, Myung-Suk ; Hwang, Baik ;
Korean Journal of Medicinal Crop Science, volume 9, issue 1, 2001, Pages 8~14
The purpose of this research was to investigate the effects of extracts from cultured hairy roots of R. undulatum on human kidney epithelial cells. The cytotoxicity was measured by colorimetric assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), neutral red (NR) and sulforhodamine protein B (SRB) with human kidney epithelial cell lines A498. MTT, NR and SRB quantities decreased propotionally in cultured A498 cells treated with the water or chloroform extracts of cultured hairy roots at increasing concentrations. These results suggest that extracts of cultured hairy roots are cytotoxic on human epithelial cells. The cytotoxicity of chloroform fraction was stronger than that of water fraction. The values of
of the extracts of chloroform fraction and those of water fraction were measured to be
, in A498 cell line.
In vitro Propagation of Trichosanthes kirilowii Max.
Hwang, Sung-Jin ; Hwang, Baik ;
Korean Journal of Medicinal Crop Science, volume 9, issue 1, 2001, Pages 15~20
Adventitious shoot buds, without an intervening callus phase, were induced from stem explants of Trichosanthes kirilowii on MS medium supplemented with 2 mg/L BA. The culture medium, carbon source as well as plant growth regulators were found to influence further shoot multiplication and eventual shoot growth. A maximum number of shoots was obtained on a MS medium containing 0.5 mg/L BA and 3% sucrose. Shoot produced in vitro were rooted on half-strength phytagelled 1/2MS medium prior to transfer to green house condition.
Determination of Ginseng Saponins by Reversed-Phase High Performance Liquid Chromatography
Kim, Cheon-Suk ; Kim, Se-Bong ;
Korean Journal of Medicinal Crop Science, volume 9, issue 1, 2001, Pages 21~25
Major saponins in ginseng were analysed using reverse phase high performance liquid chromatography with binary mobile phase gradient control system instead of normal phase column. The optimum condition were as following : reverse phase column;
), methyl cyanaide/water binary mobile phase gradient control system, solvent flow rate; 1.5 ml/min, and UV(
) detector. The complete separation of ginsenoside
was achieved within 55 min. The Regression coefficients of the calibration curves for seven ginsenosides were 0.99.
Effect of Anaerobic Treatments on the
acid and Quality of Green Tea(Camellia sinensis var. sinensis)
Park, Jang-Hyun ;
Korean Journal of Medicinal Crop Science, volume 9, issue 1, 2001, Pages 26~32
The contents of chemical components such as total nitrogen, total amino acid and vitamin C were somewhat higher in
gas treatment at
for 3 hours than those of other treatments. However, the contents of tannin and chlorophyll were slightly lower than that in the other treatments. The content of
gas treatment was higher
times with values of
than in control (35mg/100g). The scores of sensory test was not different between
gas treatment for 3 hours and control. The contents of chemical components such as total nitrogen, total amino acid including theanine and caffeine were slightly higher in
gas treatment at
for 3 hours than those of other treatments. However, the contents of tannin and vitamin C were slightly lower than those of other treatment. The content of GABA in tea leaves treated with
gas was higher
times with values of
than in control (35mg/100g). The sensory test was lower in
point) than in control(80.4 point). The contents of chemical components were not different between
gas treatment at
for 3 hours and control. Whereas the contents of chemical components were somewhat lower in
gas treatment for 1 hour and 5 hours than in control. The content of GABA in tea leaves treated with
gas was higher
times with values of
than in control(35mg/100g). The sensory test was lower in
gas treatment (
point) than in control(80.4 point). Consequently, tea mading within 5 time
gas treatment at
or 3 time
gas treatment at 20,
after plucking was considered to be the best green tea in terms of functional nature as well as taste nature.
Effect of Nitrogen Split Application on Growth and Quality of Okro Tea Leaves(Camellia sinensis var. sinensis)
Lim, Keun-Cheol ; Park, Jang-Hyun ;
Korean Journal of Medicinal Crop Science, volume 9, issue 1, 2001, Pages 33~39
The split application of a nitrogen fertilizer was applied at the levels to 3, 4, 5, 6 and 7 time. The results of yield and quality of Okro tea are summarized as follows: Among five treatments, the growth and yield were somewhat more excellent in the five time split treatments compared with other treatments. The chemical components such as total nitrogen, total amino acid and vitamin C were somewhat higher in the five time split treatment compared with other treatment. However, the content of components such as tannin, caffeine, chlorophyll were not different among plots of
time split application except three time split application manuring. The content of fatty acid was producted
. Especially, the content of oleic acid, linoleic acid and linolenic acid was the higheat in the five time split application. Sixteen kinds of free amino acid including theanine as a good taste component of Okro tea were isolated and measured by HPLC. Theanine in the five time split application was higher with value of
than in other treatment. In scoring test, tea appearance and interal qualities of 3 time split application become lower than any other treatment as 80.2 point, while five time application had the highest with 84.7 point. Conquently, those results meant that five time split application was optimum split application of fertilizer for high yield and good quality of Okro tea.
Comparison of antioxidative activities from different organs of Rosa rugosa Thunb
Kim, M.J. ; Kim, J.S. ; Kim, K.E. ; Shin, K.H. ; Heo, K. ; Cho, D.H. ; Park, C.H. ; Yu, C.Y. ;
Korean Journal of Medicinal Crop Science, volume 9, issue 1, 2001, Pages 40~44
DPPH(1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity and Vitamin contents in different plant parts and harvesting time of Rosa rugosa Thunb were determined. Among the plants parts analysed, the highest DPPH radical scavenging activity in part was root
, leaf contained the highest ascorbic acid content of 2999.6 mg/100g. Fruit contained ascorbic acid (713.6 mg/ 100g) 4.2 times less than leaf. Vitamin contents in leaf parts also differed depanding on harvesting time. Vitamin content in leaf harvested in November was much higher than that in September. Spectrophotometric analysis of total
in leaf showed lower contents than that in fruit.
Biological Activities in roots of Glycyrrhiza uralensis Fisch
Chung, Woo-Teak ; Lee, Seo-Ho ; Cha, Moon-Suk ; Sung, Nak-Sul ; Hwang, Baek ; Lee, Hyeon-Yong ;
Korean Journal of Medicinal Crop Science, volume 9, issue 1, 2001, Pages 45~54
The biological activities of ethanol, ethanol: water(1 : 1v/v) and water extracts from Glycyrrhiza uralensis Fisch, glycyrrhizin and enzymatically hydrolyzed glycyrrhizin were compared. About 50% of the growth of MCF7, A549, Hep3B and AGS cells were inhibited in adding 1.0 g/L of the crude extracts, glycyrrhizin and enzymatically hydrolyzed glycyrrhizin. For example, the ethanol extract inhibited 76%, 66% in MCF7 and Hep3B cells by adding 1.0 g/L. For cytotoxicity on human normal liver cell(WRL-68), the crude extracts were scored as above 26%. For the result of antimutagenecity using CHO V79 cell, the crude extracts proved more effective than other samples. The growth of human immune B and T cells were enhanced up to
times by adding the crude extracts. In inhibitory effect of
activity was showed that the ethanol extract, water extract and ethanol: water (1 : 1v/v) extract were appeared 65%, 68%, 62% in adding 1.0 g/L. The higher enhancement of glutathione -S-transferase activity was observed in the ethanol extract as 257% compared to the control in adding 1.0 g/L. From the results, the biological activities of the crude extracts were equivalent or higher than glycyrrhizin and enzymatically hydrolyzed glycyrrhizin.
Effects of Peduncle Cutting on Yield and Crude Saponin Content in Anemarrhena asphodeloides Bunge
Han, Seung-Ho ; Choi, Byung-Jun ; Shin, Cheol-Woo ; Son, Seok-Young ; Park, Sang-Il ;
Korean Journal of Medicinal Crop Science, volume 9, issue 1, 2001, Pages 55~61
In this study, effects of wiling peduncle on production yield and crude saponin content in Anemarrhena asphodeloides Bunge were investigation. The peduncle cutting were conduct on May 30, June 15, June 30, non-cutting of seed propagation type and young plant propagation type in Anemarrhena asphodeloides Bunge. The highest rhizome yield was 276.9g/plant on May 30, cutting puduncle in seed propagation type. The rhizome yield at seed propagation type was increased about 40.8%, compared to that at non-cutting. Crude saponin content in rhizome was 7.50% on May 30, cutting puduncle in seed propagation type. The crude saponin content at seed propagation type was increased about 32.2%, compared to that at non-cutting.
Effects of Composition and Concentration of Media on the Acclimation of the Shoots of Scrophularia buergeriana Miquel Produced in Bioreactor
Hahn, Suk-Hoon ; Chae, Young-Am ;
Korean Journal of Medicinal Crop Science, volume 9, issue 1, 2001, Pages 62~67
This study was carried out to investigate the optimum composition and concentration of medium for the acclimation of the shoots derived from bioreactor culture of Scrophularia buergeriana. Rooting and growth of the shoots cultured in bioreactor for 4 weeks were better on liquid full strength MS medium while one-tenth of MS medium was good for rooting and growth of the shoots cultured in bioreactor for 6 weeks. Leaf expansion was more effective in the shoots cultured for 6 weeks on solid MS medium than in those cultured for 4 weeks. No leaf expanded in liquid medium. Vitrification rate was higher in full strength solid MS medium than in half strength MS medium. It is concluded that acclimation of the shoots is more effective on the half strength solid MS medium containing 1.2% agar.
Effects of Seed Treatments for Promoting Seedling Emergence of Codonopsis lanceolata Trautv.
Kang, Jin-Ho ; Shim, Young-Do ; Jeong, Jong-Il ;
Korean Journal of Medicinal Crop Science, volume 9, issue 1, 2001, Pages 68~75
Reasonable seedling emergence is a prerequisite for successful crop cultivation especially in medicinal crops. The study was carried out to model the pretreatment or seed treatment of Codonopsis lanceolata seeds by evaluating the treatment effects of priming,
, drying and water imbibition after drying on their germination and then their successive seed treatment on the basis of its seedling emergence. The priming using
treatment under their different concentrations and light quality illuminated for 12 hours a day were separately done and the two best results from priming and
treatments were compared to determine the better one. The drying of imbibed seeds using the above best result and water imbibition of the dried seeds were successively done to measure the rates of germination and emergence. On the greatest germination rate of the first two individual treatments, priming was obtained at
150 mM under blue, red light or darkness for 2 days but
was done at 0.1 mM under blue light imposed for 3 days. However, the result of the latter
treatment was better than that of priming.
treated seeds were best desiccated under
and 4 hour red light illumination. A day water imbibition immediately before sowing increased the germination rate of seeds dried after
treatment. Seedling emergence tested after all 3 successive treatments,
, drying and water imbibition before sowing was greater than the two others, only
treatment and the combination of
and drying, meaning that its pretreatment of seeds or seed treatment must follow the successive procedure of the above 3 ones.
Effects of Soil Physical properties on Growth in Wasabia japonica Matsum
Byeon, Hak-Soo ; Seo, Jeong-Sik ; Lim, Soo-Jeong ; Heo, Su-Jeong ; Seo, Sang-Myung ;
Korean Journal of Medicinal Crop Science, volume 9, issue 1, 2001, Pages 76~82
This study was conducted to find out the optimum ranges of soil physical properties for wasabi growth by the relationship of soil physical properties and plant growth. Soil bulk density and hardness were higher in decomposition of granite and river sand than hydroball. This root distribution of surface layer was higher in decomposition of granite and river sand than hydroball. Growth characteristics and yield were higher in hydroball than decomposition of granite and river sand. In inlet site, the marketable rhizome weight in decompasition of granite, river sand, hydroball were 298kg/10a, 401kg/10a, 766kg/10a, respectively. But outlet, the weight in three soils were 251kg/10a, 256kg/10a, 633kg/10a, respectively.