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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Maxillofacial Plastic and Reconstructive Surgery
Journal Basic Information
Journal DOI :
Korean Association of Maxillofacial Plastic and Reconstructive Surgeons
Editor in Chief :
Volume & Issues
Volume 31, Issue 6 - Nov 2009
Volume 31, Issue 5 - Sep 2009
Volume 31, Issue 4 - Jul 2009
Volume 31, Issue 3 - May 2009
Volume 31, Issue 2 - Mar 2009
Volume 31, Issue 1 - Jan 2009
Selecting the target year
TOPICAL GENE DELIVERY TO NORMAL ORAL EPITHELIUM USING ADENOVIRUS IN ORGAN CULTURE MODEL
Kim, Tae-Hwan ; Kwak, Myung-Ho ; Lee, Choon-Ho ; Park, Jun-Woo ; Park, Young-Wook ; Kim, Seong-Gon ;
Maxillofacial Plastic and Reconstructive Surgery, volume 31, issue 3, 2009, Pages 193~197
Background: Though it is clear that many types of viruses can infect the oral mucosa, its condition for infection is unclear. The purpose of this study was to analyze the conditions for viral infection of normal oral mucosa and explore the possibility of topical gene therapy to oral mucosa using a viral vector. Methods: Freshly taken fragments of the palate and the tongue of mice were used for organ culture. The specimens were exposed to green fluorescent protein (GFP)-adenoviral vector for 1 hour except for the control. Initial viral titer was
and the virus was diluted to working concentrations. The dilution ratio was 1:1,000 (
), 1:10,000 (
), and 1:100,000 (
). They were then cultured on a stainless steel wire mesh in an organ culture dish. The specimens were stereoscopically examined every 24 hours for 6 days, after which they were fixed and analyzed through immunohistochemical methods Results: There was no visible expression in the control,
groups. Initial expression was observed at 24 hours after infection in both the palate and the tongue in
and the expression significantly increased until 3 days in the palate and 2 days in the tongue after infection (P<0.05). In both groups, the expression was mostly observed at the resection margin. Immunohistochemical studies showed that the epithelial cells were positive to GFP. Conclusion: The present study showed that topically applied adenovirus containing specific genetic information of GFP could successfully transduce GFP in normal oral epithelial cells at the resection margin in organ culture in terms of dose and exposure time.
MANDIBULAR BONE REGENERATION USING AUTOGENOUS SKIN-DERIVED PRECURSOR CELLS WITH A MIXED DEMINERALIZED BONE AND FIBRIN GLUE SCAFFOLD IN MINIATURE PIGS
Byun, June-Ho ; Choi, Mun-Jeong ; Choi, Young-Jin ; Shim, Kyoung-Mok ; Kim, Uk-Kyu ; Kim, Jong-Ryoul ; Park, Bong-Wook ;
Maxillofacial Plastic and Reconstructive Surgery, volume 31, issue 3, 2009, Pages 198~206
Purpose: The aims of this study were to assess the in vitro co-culturing pattern of isolated skin-derived precursor cells (SKPs) with a mixed demineralized bone (DMB) and fibrin glue scaffold and to evaluate in vivo osteogenesis after transplantation of autogenous SKPs with a these mixed scaffold in the animal's mandibular defects. Materials and Methods: We isolated SKPs from the ears of adult 4 miniature pigs. The isolated SKPs were co-cultured with a mixed DMB and fibrin glue scaffold in a non-osteogenic medium for 1, 2, and 4 weeks. Histological characteristics of in vitro co-cultured cells and scaffold were evaluated.
of autogenous porcine SKPs were grafted into the mandibular defects with a DMB and fibrin glue scaffold. In the control sites, only a scaffold was grafted, without SKPs. After two animals each were euthanized at 2 and 4 weeks after grafting, the in vivo osteogenesis was evaluated with histolomorphometric and osteocalcin immunohistochemical studies. Results: Homogeneously shaped skin-derived cells were isolated from porcine ear skin after 3 or 4 weeks of primary culture. In vitro osteogenic differentiation of SKPs was observed after co-culturing with a DMB and fibrin glue scaffold in a non-osteogenic medium. Von Kossa-positive bone minerals were also noted in the co-cultured medium at 4 weeks. As the culture time progressed, the number of observable cells increased. Trabecular new bone formation and osteocalcin expression were more pronounced in the SKP-grafted group compared to the control group. Conclusion: These findings suggest that autogenous SKP grafting with a DMB and fibrin glue scaffold can serve as a useful alternative to bone grafting technique.
THE HISTOLOGIC STUDY OF BONE HEALING AFTER HORIZONTAL RIDGE AUGMENTATION USING AUTO BLOCK BONE GRAFT
Oh, Jae-Kwen ; Choi, Byung-Jun ; Lee, Baek-Soo ;
Maxillofacial Plastic and Reconstructive Surgery, volume 31, issue 3, 2009, Pages 207~215
Purpose: The aim of the present study is to evaluate the long term bone healing after horizontal ridge augmentation using auto block bone graft for implant installation timing. Materials and Methods: Five Beagle dogs(which were 14 months old and weighted approximately 10kg). In surgery 1(extraction & bone defect), premolars(P2, P3,P4) were extracted and the buccal bone plate was removed to create a horizontally defected ridge. After three months healing, in surgery 2(ridge augmentation). Auto block bone grafts from the mandibular ramus were used in filling the bone defects were fixed with stabilizing screws. The following fluorochrome labels were given intravenously to the beagle dogs: oxytetracycline 1week after the surgery, alizarin red 4 weeks after the surgery, calcein blue 8 weeks after the surgery. The tissue samples were obtained from the sacrificed dogs of 1, 4, 8, 12, 16 weeks after the surgery. Non-decalcified sections were prepared by resin embedding and microsection to find thickness of
for the histologic examination and analysis. Results: 1. We could achieve the successful reconstruction of the horizontal bone defect by auto block bone graft. The grafted bone block remained stable morohologically after 16 weeks of the surgery. 2. In the histologic view. We observed osteoid tissue from the sample
week sample and active capillary reconstruction in the grafted bone from the
week sample. Healing procedures of auto bone grafts were compared to that of the host bone. 3. Bone mineralization could be detected from the
week sample. 4. Fluorochrome labeling showed active bony changes and formation at the interface of the host bone and the block graft mainly. Bony activation in the grafted bone could be seen from the
week samples. Conclusions: Active bone formation and remodeling between the grafted bone and host bone can be seen through the revascularization. After the perfect adhesion to host bone, Timing of successful implant installation can be detected through the ideal ridge formation by horizontal ridge augmentation.
HEALING PATTERNS OF THE ACELLULAR DERMAL MATRIX DEPEND ON GRAFT METHOD IN THE RABBIT EARS
Ryu, Jae-Young ; Ryu, Sun-Youl ;
Maxillofacial Plastic and Reconstructive Surgery, volume 31, issue 3, 2009, Pages 216~221
Purpose: The retention of the basement membrane complex, which was the unique feature of the acellular dermal matrix (
), plays an important role in the normal process of wound healing. The present study was aimed to compare the healing of the acellular dermal matrix according to the graft method in the rabbit ear. Materials and methods: Six mature rabbits weighing about 3.0 kg were used,
sized subcutaneous pockets were created between the ear skin and the underlying perichondrium. In the control group, the acellular dermal matrix was grafted with the basement membrane facing toward the perichondrium. On the contrary, the acellular dermal matrix was grafted with the basement membrane facing toward the skin side in the experimental group I. In the experimental group II, the acellular dermal matrix was grafted like rolled configuration with basement membrane side in. The grafted site was picked at 3, 7, and 21 days after the graft. Serial sections were processed by H-E stain and examined under light microscopy to assess the healing patterns. Results: There was no distinct volume loss in the gross examination, but resorption was observed from the edge of the acellular dermal matrix in the histological examination. The space of resorption was replaced by the newly formed fibrous tissues and vessels. The inflammatory cells were more increased at 7 days after the graft than the early days. However, inflammation was decreased at 21 days after the graft. Regardless of the graft direction, no differences were observed between the control and the experimental group I in the healing patterns. Conclusion: These results suggest that the acellular dermal matrix can be used simply and effectively without regard to the graft direction as a substitute of autogenous material for repairing soft tissue defect.
AN EXPERIMENTAL STUDY ON THE EFFECT OF LASER ON SOFT TISSUE HEALING
Park, Young-Wook ; Jang, Jae-Hyun ; Kim, Jung-Hwan ; Park, Jung-Min ; Lee, Suk-Keun ;
Maxillofacial Plastic and Reconstructive Surgery, volume 31, issue 3, 2009, Pages 222~228
Objectives: This study is aimed to compare the wound healing processes between conventional scapel wound and Er,Cr:YSGG (Erbium,Chromium, Yttrium, Scandium, Gallium, Garnet) laser wound using experimental animals. Experimental Design: Two types of wounds were made by linear and round incisions using scalpel and Er,Cr:YSGG laser, respectively, on the thigh of Sprague-Dawley rats. Sprague-Dawley rats were serially sacrified as follows: post operative 12, 24, 48 hours, and 3, 7, 14 days. The skin wounds were grossly and microscopically analyzed during the healing period. Result: The Er,Cr:YSGG laser incision showed better wound healing for the linear incision experiment than the scapel incision. Whereas the scapel incision showed better wound healing for the round incision experiment than the Er,Cr:YSGG linear incision. As the Er,Cr:YSGG laser damage in the round incision experiment could be much increased compared with the round incision by scapel. So, the round incisions by the Er,Cr:YSGG laser were resulted in the poor wound healing compared with those by the scapel. Conclusion: The Er,Cr:YSGG laser is more favorable for the fast linear incision, while the scapel is more favorable for the modified round incision.
PANORAMIC ANALYSIS ABOUT SPONTANEOUS BONE REGENERATION AFTER ENUCLEATION OF JAW CYST
Yim, Jeong-Hoon ; Lee, Jae-Hoon ;
Maxillofacial Plastic and Reconstructive Surgery, volume 31, issue 3, 2009, Pages 229~236
Purpose: Some recent literatures report that it is possible to recover defected areas caused by enucleation of relatively large jaw cysts without using bone grafts. The aim was to find out whether spontaneous recovery of defected area with time occurred and what the contributing factors were. Materials and methods: In total, 194 patients were considered as patients. Out of these 194 patients, 74 patients who had no wound dehiscence and who were available for follow-up studies were selected. They were classified into two groups according to the size of radiolucent area in the preoperative panoramic radiographs: in one group, it was larger than
, while in the other group, it was smaller than
. Follow-up panoramic radiographs were taken immediately after the surgery, then after 3, 6, 9 and 12 months. On those radiographs, changes in size and density of the defected areas were observed using the Gray-level histogram of Adobe photoshop v7.0. Correlation between bone regeneration and factors such as the type and size of the cysts, age, sex, site of the cysts and systemic disease was evaluated using the General repeated measure and Mann-Whitney Test. Results: Analyses of panoramic radiographs showed that the recovery of radiopacity after 12 months was more than 97% on average in defected areas that were smaller than
. in the defected areas that were larger than
, considerable portion showed recovery of radiopacity. No statistically significant change was observed in bone density according to the type of cysts. Young patients under 20 years of age with highly active metabolism presented more significant bone regeneration than patients over 20 years of age. Bone regeneration was more hampered in patients who had medical disease, compared with patients who didn’t have any medical problem. No statistically significant change was seen in bone density according to sex. Changes in bone density according to the site of cysts such as maxilla, mandible, anterior or posterior region were not considered to be significant. Conclusion: Analyses of panoramic radiographs suggest that in approximately 12 months after the enucleation of cysts, clinically acceptable spontaneous bone regeneration can be observed even though normal bone graft procedures have not been applied.
FOUR-YEAR SURVIVAL RATE OF RBM SURFACE INTERNAL CONNECTION NON-SUBMERGED IMPLANTS AND THE CHANGE OF THE PERI-IMPLANT CRESTAL BONE
Jeon, Hye-Ran ; Kim, Myung-Rae ; Lee, Dong-Hyun ; Shin, Jung-Sub ; Kang, Na-Ra ;
Maxillofacial Plastic and Reconstructive Surgery, volume 31, issue 3, 2009, Pages 237~242
Implant-supported fixed and removable prostheses provide a proper treatment modality with reliable success. The SS
Implants is a one-stage nonsubmerged threaded titanium implants with Resorbable Blasting Media (RBM) surface developed by Osstem company (Busan, Korea) in October of 2002. This study is to evaluate the survival rate of the SS
Implants for 4 years using radiographic parameters and to review the retrieved implants by the cytotoxicity tests. Since September 2003, 439 SS
implants had been used for 173 patients at Ewha Womans University Medical Center in Korea. Patients consisted of 91 females (52.6 %) and 82 males (47.4 %). The patients' mean age was
years, ranging from 21 to 83 years. The follow-up period ranged from 9 to 46 months (mean F/U
months). The results are as follows; 1. Of 439 implants, 17 implants were removed and 4-year cumulative survival rate was 96.1%. 2. 82.3% of 17 failed implants were founded during healing phase, and 94.1% of failed fixtures were removed within 5 months after implantation. 3. Crestal bone around the implants was resorbed to 1 mm in 89.0%, to 1 - 2 mm loss of the marginal bone in 8.3%, and the bone loss over 2 mm was occurred in 2.7%. 4. Microscopic examination of the retrieved implants disclosed Grade 0 cytotoxicity in 4 and Grade 1 cytotoxicity in 2 of 6 groups divided according to LOT numbers. Inhibition rate with optical density was acceptable as low as ISO standard.
SINUS FLOOR GRAFTING USING CALCIUM PHOSPHATE NANO-CRYSTAL COATED XENOGENIC BONE AND AUTOLOGOUS BONE
Pang, Kang-Mi ; Li, Bo-Han ; Alrashidan, Mohamed ; Yoo, Sang-Bae ; Sung, Mi-Ae ; Kim, Soung-Min ; Jahng, Jeong-Won ; Kim, Myung-Jin ; Ko, Jea-Seung ; Lee, Jong-Ho ;
Maxillofacial Plastic and Reconstructive Surgery, volume 31, issue 3, 2009, Pages 243~248
Purpose: Rehabilitation of the edentulous posterior maxilla with dental implants often poses difficulty because of insufficient bone volume caused by pneumatization of the maxillary sinus and by crestal bone resorption. Sinus grafting technique was developed to increase the vertical height to overcome this problem. The present study was designed to evaluate the sinus floor augmentation with anorganic bovine bone (Bio-
) using histomorphometric and clinical measures. Patients and methods: Thirteen patients were involved in this study and underwent total 14 sinus lift procedures. Residual bone height was
. Lateral window approach was used, with grafting using Bio-
only(n=1) or mixed with autogenous bone from ramus and/or maxillary tuberosity(n=13). After 6 months of healing, implant sites were created with 3mm diameter trephine and biopsies taken for histomorphometric analysis. The parameters assessed were area fraction of new bone, graft material and connective tissue. Immediate and 6 months after grafting surgery, and 6 months after implantation, computed tomography (CT) was taken and the sinus graft was evaluated morphometric analysis. After implant installation at the grafted area, the clinical outcome was checked. Results: Histomorphometry was done in ten patients.Bio-
particles were surrounded by newly formed bone. The graft particles and newly formed bone were surrounded by connective tissue including small capillaries in some fields. Imaging processing revealed
of new bone,
connective tissue, and
of remaining Bio-
particles. All grafted sites received an implant, and in all cases sufficient bone height was achieved to install implants. The increase in ridge height was about
immediately after operation (from 13mm to 19mm). After 6 months operation, ridge height was reduced about
. After implant installation, average marginal bone loss after 6 months was
. Conclusion: Bio-
showed new bone formation similar with Bio-
histomorphometrically and appeared to be an effective bone substitute in maxillary sinus augmentation procedure with the residual bone height from 2 to 6mm.
USE OF CORTICOSTEROIDS IN THE TREATMENT OF LOCALIZED LANGERHANS CELL HISTIOCYTOSIS OF THE MANDIBLE : CASE REPORT
Choi, Jung-Yong ; Lee, Sang-Hwa ; Yoon, Hyun-Joong ;
Maxillofacial Plastic and Reconstructive Surgery, volume 31, issue 3, 2009, Pages 249~253
Langerhans cell histiocytosis(LCH), previously known as histiocytosis X, is rare, proliferative disorder in which the accumulation of pathologic Langerhans cell leads to tissue infiltration and destruction. The jaw is involved 10~20% of all LCH and most common oral symptoms are jaw swelling or a palpation mass. Most of subjects are males and disease appears to start before the age of 10 years. An 11 years old girl was referred from pediatric department. Histological examination confirmed the diagnosis LCH. She was treated intra-lesional injection of corticosteroid. The overall outcome was excellent. After a follow-up period of 20 month, the patient present with no evidence of residual disease on Panoramic view and whole body bone scan. We report this early recognized LCH case treated by corticoid injection in good progress with literature review.
MAXILLARY SINUS BONE GRAFT USING PARTICULATED RAMAL AUTOBONE AND BOVINE BONE
Kim, Kyoung-Won ; Lee, Eun-Young ;
Maxillofacial Plastic and Reconstructive Surgery, volume 31, issue 3, 2009, Pages 254~261
The maxillary sinus bone graft procedure is one of the predictable and successful treatments for the rehabilitation of atrophic and pneumatized edentulous posterior maxilla. Materials used for maxillary sinus floor augmentation include autogenous bone, allogenic bone, xenogenic bone and alloplastic materials. Among them, autogenous bone grafts still represents 'gold standard'for bone augmentation procedures. We selected the mandibular ramus area as a donor site for the autogenous bone graft because of low donor site morbidity. We performed maxillary sinus bone graft procedures with implant placement using particulated ramal autobone and bovine bone mixture, and got good results. This is a preliminary report of the maxillary sinus bone graft using particulated ramal autobone and bovine bone, requires more long-term follow up and further studies.
SOLITARY FIBROUS TUMOR IN BUCCAL CHEEK : CASE REPORT
Choi, Min-Hye ; Yoon, Kyu-Ho ; Cheong, Jeong-Kwon ; Park, Kwan-Soo ; Shin, Jae-Myung ; Kim, Hae-Lin ; Na, Hye-Jung ;
Maxillofacial Plastic and Reconstructive Surgery, volume 31, issue 3, 2009, Pages 262~266
Solitary fibrous tumor (SFT) is a neoplasm that arises most commonly in pleura. Although SFT occasionally occurs in extrapleural locations, the incidence in the oral cavity is rare. SFT is benign in almost cases and surgical excision is the effective treatment. SFT occurred in the left cheek of a 60-year-old man presented with a painless submucosal mass. The tumor was surgically removed. Immunohistochemical study showed that tumoral cells were negative for SMA, S-100, but positive for Bcl-2, CD34. SFT is easily over-diagnosed if strict criteria are not carefully applied, and strict diagnostic criteria are necessary to avoid confusion of SFT with more aggressive lesions.
ALVEOLAR CLEFT GRAFT
Jun, Sang-Ho ; Padwa, Bonnie L. ; Jung, Young-Soo ;
Maxillofacial Plastic and Reconstructive Surgery, volume 31, issue 3, 2009, Pages 267~272
Bone grafting the alveolar cleft allows for stability and continuity of the dental arch, provides bone for eruption of permanent teeth or placement of dental implants, and gives support to the lateral ala of the nose. Closure of residual oronasal fistula can occur simultaneously. Repair of alveolar clefts can occur at a variety of stages defined as primary, early secondary, secondary, and late. Most centers perform this surgery as secondary bone grafting. Autogenous bone provides osteogenesis, osteoinduction and conduction and is recommended for grafting to the cleft alveolus and several donor sites are available. The surgeon should select the best flap design considering the amount of mucosa available, blood supply and tension-free closure, and the extent of the oronasal communication. The authors provide a comprehensive understanding of alveolar clefts and their repair by reviewing the historical perspective, objectives for treatment, timing, source of graft, presurgical orthodontics, surgical techniques, postoperative care, and complications.