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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Biomolecules and Therapeutics
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Journal DOI :
The Korean Society of Applied Pharmacology
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Volume & Issues
Volume 9, Issue 4 - Dec 2001
Volume 9, Issue 3 - Sep 2001
Volume 9, Issue 2 - Jun 2001
Volume 9, Issue 1 - Mar 2001
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The effects of Sa-Mul-Tang (Si-Wu-Tang), a Traditional Chinese Medicine, on Phenylhydrazine-induced Anemic Rats
Ryu, Jong-Hoon ; Yook, Chang-Soo ;
Biomolecules and Therapeutics, volume 9, issue 1, 2001, Pages 1~6
Sa-Mul-Tang (Si-Wu-Tang, SMT), a kind of Chinese medicine, has been used for the hemato-deficient disease for hundreds of years. In this work, investigations on the anti-anemic activity of an aqueous extract of SMT were undertaken in order to find the pharmacological basis for the ethnomedical use of the formulation. Three kinds of Angelicase species, such as Angelica sinensis, Angelica acutiloba, and Angelica gigas, were used for preparing the water extracts of SMT. Anemic model rats were induced by the treatment of phenylhydrazine (40 mg/kg/day, i.p.) for 4 days. After the treatment of phenylhydrazine, rats were divided into several groups for their different treatment of three kinds of SMT. Red blood cell (RBC), hemoglobin (Hg), and hematocrit (Hct) were determined on the day 0, 3, 6, 10, 14 after the treatment of SMTs and erythrocytes deformabilities were also determined at the end of experiments. Oral administration of SMT (1g/kg/day) for 14 days did not ameliorate drug-induced anemic states evaluated by RBC counts, Hg contents, and Hct values. However, the erythrocyte deformabilities were improved in phenylhydrazine-treated group by the administragest that SMTs (p<0.05). Especially, these effects were high in the Angelica acutiloba group. These results suggest that SMTs have an ameliorative effect on blood rheology related to the blood stasis syndrome in oriental diagnostics not on the blood deficient states related to the anemic syndrome.
Identification of the Chicken Meat Allergens
Biomolecules and Therapeutics, volume 9, issue 1, 2001, Pages 7~14
The chicken meat has been reported as one of the food causing allergic reactions predominantly to Korean. At present, several in vitro tests for immunoglobulinG (IgG)-mediated as well as IgE-mediated food allergy are available. 13 clinically chicken meat-allergic patients were investigated together with 4control subjects for identification of chicken meat-specific reactivity by ELISA. Also, protein profile and IgE, IgGtotal and IgG4-reacting allergens were detected by means of sodium dodecyl sulfate-polyacrylamide gel electro-phoresis (SDS-PAGE)and immunoblotting. Chicken meat extracts were prepared as raw, heated, heat and simulated gastric fluid (SGF) treated samples to characterize the stability of allergen to physicochemical treatment. SDS-PAGE revealed 9~200 kDa bands. And in immunoblotting 7 sera were identified most major bands between 10 and 78 kDa. In case of IgE, six proteins (17, 26, 35, 40, 78 kDa) were predominant in heat-treated extract, and the one (35 kDa) was present in SGF-treated preparations. In case of IgG
and IgG4, most of them showed a patters simmilar to IgE. There were significant differences (P<0.05) in IgE, IgG
, IgG4 Abs to chicken meat between the allergic and control subjects in ELISA. In addition, the concentration of IgG4Abs in the challenge-positive subjects was significantly higher than that of control subjects. It is considered that the specific IgE response to chicken meat was rarely prevalent to Koreans. However, the specific IgG4 response play an important role in the development of allergic symptoms.
Hair-growth Effect of Chrysin 7-O-cyclopropanecarboxylate
Biomolecules and Therapeutics, volume 9, issue 1, 2001, Pages 15~19
The derivative of chrysin 7-O-cyclopropanecarboxylate was synthesized by condensing cyclopropanecarboxylic acid with chrysin in organic solvent, and its structure was identified by NMR, MS, UV IR etc. We also investigated the physico-chemical properties, anti-diabetic effect and set up the quantitative analytical method of this compound. The correlation coefficient of calibration curve on this compound was approximately 0.9985 by absorption spectrophotometry. And, this study was carried out to investigate the hair-growth effect of chrysin derivative to the black mouse (C57BL/6). When this derivative in ethanol solution was administered to the back of mouse by method of skin paste, this derivative promoted the hair growth of mouse.
A Study on the Serotonin Metabolism and the Morphine-related Analgesic Mechanism in Mice Fed Tryptophan Supplemented Deit (II)
Biomolecules and Therapeutics, volume 9, issue 1, 2001, Pages 20~25
In this study we fed control diet and tryptophan supplemented diets containing 0.35% tryptophan to ICR mice for 2 weeks. The concentrations of serotonin and 5-HIAA were changed by injection of the serotonin synthesis inhibitor, p-CPA and the serotonin precursor, serotoninP and the change of brain serotonin concentration negatively correlated with that of pain sensitivity, and p-CPA and serotoninP also changed the analgesic effect of morphine. The injection of naloxone, the opiate antagonist, resulted in an increase in the writhing frequency, but its antagonistic effect was not significant. The concentration of 5-HIAA elevated in mice brain at least 3hr after administration of morphine hydroxide indicates that the changes in brain serotonin metabolism may be associated with the acute effects of morphine analgesia. In short, these results not only suggest that tryptophan supplemented diet suppress pain sensitivity in mice, but also indicate that at least in part analgesic mechanism of serotonin may be associated with morphine analgesia.
Effect of Glibenclamide,
Channel Blocker, on Renal Function in Rabbit
Biomolecules and Therapeutics, volume 9, issue 1, 2001, Pages 26~32
This study was investigated about the effect of glibenclamide (GLY) which is
channel blocker on renal function in rabbit, GLY, when given into the vein, produced the diuretic action accompanied with the increases of amounts of N
excreted into urine (
), and then osmolar and negative free water clearances (
), fraction excretory rates of filtered N
) and ratios of
were augmented. Filtration fraction (FF) were reduced because renal plasma flow (RPF) were not changed but glomerular filtration rates (GFR) were diminished. GLY administered into a renal artery exhibited significant reduction of urine volume along with the decreases of GFR and RPF in only experimented kidney whereas changes of renal function was not observed in control kidney. GLY given intracerebroventricularly exhibited diuretic action along with the increase of
by small dose which was not affect on renal function when it given into the vein. Above results suggest that GLY given into the vein in rabbit produce the diuretic action by inhibition of electrolytes reabsorption in renal tubules through central function. function.n. function.ion.
Efficacy of Physostigmine and Procyclidine as Combinational Prophylactics of Organophosphate Poisoning
Biomolecules and Therapeutics, volume 9, issue 1, 2001, Pages 33~39
Antidotal efficacy of combinational prophylactics composed of physostigmine plus procyclidine, alone or in combination with antidotes such as atropine plus 2-pralidoxime or atropine plus HI-6, was evaluated in rats. Physostigmine (0.1 mg/kg) plus procyclidine (3 mg/kg), pretreated subcutaneously 30 min prior to subcutaneous exposure to organophosphates of militarily importance, exerted protection ratios of 7.2, 6.5, 4.0, 2.9 and 8.0 fold for tabun, saris, soman, cyclosarin and V-agent, respectively. In comparison, low effects (1.7 fold for soman and 1.3 fold for cyclosarin) were achieved with the traditional antidotes atropine (17.4 mg/kg) plus 2-pralidoxime (30 mg/kg) administered intramuscularly immediately after organophosphate, in contrast to high effects (5.5 fold for soman and 160.0 fold for cyclosarin) with atropine (17.4 mg/kg) plus HI-6 (125 mg/kg), although the protection ratio markedly decreased when treatment of antidotes was delayed. Note- worthy, the combinational prophylactics markedly potentiated the effects of antidotes to higher than 5.0 fold in all cases. In addition, the combinational prophylactics fully prevented the seizures and excitotoxic brain injuries induced by a high dose (100 mg/kg, 1.3 LD
) of soman. Taken together, it is suggested that the prophylactics composed of physostigmine and procyclidine, in combination with posttreatment antidotes, could be a promising regimen for the prevention of lethality, seizures and brain injuries induced by organophosphates possessing diverse properties.s.
Effect of Steroid Hormones and Intracellular
on Taurine Transporter Activity in Murine Macrophage Cell Line
Biomolecules and Therapeutics, volume 9, issue 1, 2001, Pages 40~45
The activity of taurine transporter is affected by various extracellular stimuli such as ion, hormone and stress. To assess effects of steroid hormones antral cyclosporin A (CsA) on the taurine transporter activity, murine monocytic RAW264.7 cell line was stimulated with dexamethasone (DM), triamcinolone (TA), cortisone (CS), hydrocortisone (HCS), prednisone (PSN), prednisolone (PSL) and methylprednisolone (MPSL) in the presence of 12-0-tetradecanoylphorbol-13-acetate(TPA). Treatment of TPA on the cell line led to significant reduction of taurine transporter activity. However, in case of stimulation of the cells with steroid hormones in the presence of TPA, all of them recovered TPA-induced reduction of the taurine transporter activity. Treatment of the cells with CsA led to significant reduction of the taurine transporter activity. Ionomycin (IM) recovered the reduced taurine transporter activity by CsA, but failed in the presence of EDTA, a calcium chelating agent. These results showed that glucocorticoid hormone recovered TPA-induced reduction of taurine transporter activity and that IM recovered CsA-induced reduction of the transporter activity by increasing intracellular free
Acute Oral Toxicity Studies of Extract of Sanghwang Mushroom (Phellinus linteus)
Biomolecules and Therapeutics, volume 9, issue 1, 2001, Pages 46~50
The current study was performed to determine the acute oral toxicity of a crude extract of sanghwang mushroom (Phellinus linteus), in SD rats. 5 rats of each sex were orally treated with a single dose of extract of sanghwang mushroom at doses of 0, 500, 1,000, 2,000 mg/kg, respectively. After the treatment, clinical signs and body weight change, the food and water consumption were observed for 14 days. All animals survived during the study and did not show any clinical signs. Body weight gain showed no significant difference between the control and treated rats. However, body weight gain delayed in high dose group (2,000 mg/kg) on day 1~3 after administration. Another 5 rats of each sex were orally treated with a single dose of extract of sanghwang mushroom at dosages 4,000, 5,000 mg/kg respectively, but all animals survived during the study and did not show any clinical signs. It is suggested that LD
of extract of sanghwang mushroom by oral administration was estimated to be over 5,000 mg/kg in both sexes of rats.s.
Pharmacological Effects of Extract of Bufonis Yenenum
Biomolecules and Therapeutics, volume 9, issue 1, 2001, Pages 51~54
Bufonis Venenum is a toad venom and its main components are bufadienolides, namely resibufogenin, bufalin and cinobufagin. The desensitizing effect of Bufonis Venenum is useful for the treatment of the premature ejaculation in Chinese medicine. But, minor components of Bufonis Venenum cause problems such as topical burring, pain, and erectile dysfunction. To clarify and eliminate the components responsible for these side effects, we prepared two extracts of Bufonis Venenum with either 70% ethanol or ethylacetate and tested their pharmacological effects. The extract of Bufonis Venenum with 70% ethanol produced pain response in rat hind paw, and exhibited contraction of rabbit corpus cavernosal muscle in vitro. On the other hand, the ethylacetate extract did not cause pain and smooth muscle contraction. The desensitizing effect of the ethylacetate extract was similar to that of the 70% ethanol extract. In conclusion, these results show that the extract of Bufonis Venenum with ethylacetate does not have the components causing side effects and deserve further study for therapeutic potential in premature ejaculation in men.
General Pharmacology of CJC-50100, a Recombinant Hepatitis B Virus Surface Antigen
Biomolecules and Therapeutics, volume 9, issue 1, 2001, Pages 55~62
CJC-50100 is a recombinant hepatitis B virus surface antigen (HBsAg) expressed in yeast. The general pharmacological properties of CJC-50100 were evaluated in mice, rats, dogs and isolated guinea pig ileum. The doses were 0.33~33.3
g/kg i.m. for mice and rats and 3.3~9.9
g/kg i.v. for dogs. The concentrations of 0.002~0.02
g/ml were used for the assay with guinea pig ileum. Intramuscular administration of CJC-50100 at the doses did not alter general behavior and the responses for central nervous system, smooth muscle, gastrointestinal system, cardiovascular and respiratory system, and water and electrolytes excretion. In summary, CJC-50100 had no pharmacological effect in these studies even up to the 100-fold of the expected clinical dose, 20
General Pharmacology of (R)-JG-381, A New Antidiabetic Agent
Biomolecules and Therapeutics, volume 9, issue 1, 2001, Pages 63~68
General pharmacological properties of (R)-JG-381 were examined in laboratory animals to investigate its safety profile. Administration of (R)-JG-381 (50 and 100 mg/kg) in mice and rats had no effects of general behaviors, central nervous system of the animals in test systems of pentobarbital-induced sleeping time, writhing syndromes induced by 0.7% acetic acid, chemo-shock produced by pentylenetetrazole, and, however, had mild effects on motor coordination. Heart rate and blood pressure were not changed by (R)-JG-381 treatment. (R)-JG-381 also showed mild effects on intestinal propulsion and gastric secretion. These results suggest that (R)-JG-381 dose not exert serious pharmacological effects.