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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Research in Plant Disease
Journal Basic Information
Journal DOI :
Korean Society of Plant Pathology
Editor in Chief :
Volume & Issues
Volume 10, Issue 4 - Dec 2004
Volume 10, Issue 3 - Sep 2004
Volume 10, Issue 2 - Jun 2004
Volume 10, Issue 1 - Mar 2004
Selecting the target year
Review of Disease Incidence of Major Crops in 2003
Kim, Choong-Hoe ;
Research in Plant Disease, volume 10, issue 1, 2004, Pages 1~7
DOI : 10.5423/RPD.2004.10.1.001
The year of 2003 was characterized as a cool humid year. Low temperature and frequent rains were continued during March to July, resulting in 1.6 times higher rainfalls and 32％ less sunshine period compared to the average yean Due to 2003`s climatic condition, rice blast, and bacterial leaf blight occurred severely. Higher rainfalls caused severe epidemic of phytophthora disease and, in case of red-pepper, 55％ of cultivation acreage was devastated by the disease over the country. Besides, crop diseases which become severe under cool-humid conditions, such as gray mold, sclerotinia rot, downy mildew, increased significantly compared to the previous year. In fruit trees, brown spot of apple, and pear scab occurred severely causing much yield loss.
Root Rot of Panax Ginseng Caused by Serratia liquefaciens
Research in Plant Disease, volume 10, issue 1, 2004, Pages 8~12
DOI : 10.5423/RPD.2004.10.1.008
The diseased ginseng plants, their trunks fall down anil roots rot, were observed in ginseng cultivation field at Bongwha, Kyungbuk. Inoculation of the bacterium isolated from root rot lesion induced a range of symptoms on leaves, trunks and roots; The bacterium caused wilting with chlorosis and black discoloration on leaves, empty of inside trunks and rot on roots. The bacterium was identified as Serritia liquefaciens based on the morphologcal and physiological characteristics. This is the first report in Korea on roots rot of ginseng occurred by S. liquefaciens.
Potato Basal Stem Rot Caused by Pythium myriotylum in Hydroponic Cultural System
Hong, Soon-Yeong ; Kim, Jin-Won ; Kang, Yong-Kil ; Yang, Young-Moon ; Kang, Hyeong-Sik ;
Research in Plant Disease, volume 10, issue 1, 2004, Pages 13~16
DOI : 10.5423/RPD.2004.10.1.013
A basal stem rot disease of potato (Solanum tubersum) grown on hydroponic system in greenhouse was found in Jeju-city, Jeju Province of Korea in January 2001. The symptoms occurred on basel stem and root, and than plants eventually were died. The casual fungus isolate from basal stem rot symptom and identified to be Pythium myriotylum on the basis of mycological characteristics; Main hyphae wide were 8.4
in size. Oogonia were spherical, smooth, mostly terminal in shape and 26.4∼31.2
in size. Antheridia were 9.6∼14,4
in size. Oospores were spherical, smooth, aplerotic in shape and 21.6∼26.4
in size. This is the first report on the basal stem rot of potato caused by P. myriotylum in Korea.
Foliage Blight of Vinca (Catharanthus roseus) by Phytophthora nicotianae
Lim, Yang-Sook ; Choi, Chung-Don ; Kim, Byung-Soo ;
Research in Plant Disease, volume 10, issue 1, 2004, Pages 17~20
DOI : 10.5423/RPD.2004.10.1.017
A disease causing blights on leaves, stems, flowers, and pods of Vinca (Catharanthus roseus L.) was occurred in Aug. 2003 in Chengdo Peach Experiment Station. A species of Phytophthora was isolated from the diseased tissues. The causal fungus was identified as Phytophthora nicotianae on the basis of mycological characteristics and pathogenicity. Sporangia were ovoid to spherical, noncaducous, prominently papillate and averaged 38.0
in dimension. Chlamydospores were abundantly produced on agar media and sized about 30.5 fm in diameter, The fungus was heterothallic and Al mating type. Oospores were measured 23.1
in size. Optimum temperature for growth of the fungus was 25 to 3
. This is the first report of occurrence of foliage blight of Vinca caused by P. nicotianae in Korea.
Stem Rot of Capsicum annuum Caused by Sclerotium relfsii in Korea
Kwon, Jin-Hyeuk ; Park, Chang-Seuk ;
Research in Plant Disease, volume 10, issue 1, 2004, Pages 21~24
DOI : 10.5423/RPD.2004.10.1.021
A destructive stem rot of pepper (Capsicum annuum) was found from the often field sporadically in Jingyemyon, Hadong-gun in July 2002 and vinyl houses in Moonsan-eup, Jinju City in October 2003. The same fungus also caused collar and crown rot and systemic wilt or blight of whole plant. White mycelium spread over stems of infected plants and sclerotia formed on the old lesions and near the soil surface. The fungus showed maximum mycelial growth around 3
. The mycelial color is white and width of hyphae ranges 3.6∼10.2
, and formed clamp connection. Numerous sclerotia were farmed in artificial media such as PDA at 3
. The shape of sclerotia were sphere and 1.0∼2.1 mm in diameter, The fungus was isolated repeatedly from the infected tissues and the pathogenecity of fungus to pepper (Capsicum annuum) was confirmed, and identified as Sclerotium rolfsii. This is the first report on the stem rot of pepper (Capsicum annuum) caused by Sclerotium rolfsii in Korea.
Occurrence of Root-knot Nematode on Dendranthema x grandiflorum and Influences on Plant Growth
Kim, Dong-Geun ; Kim, Hyun-Seok ; Kim, Seung-Han ; Lee, Jung-Hwan ; Yoon, Jae-Tag ;
Research in Plant Disease, volume 10, issue 1, 2004, Pages 25~29
DOI : 10.5423/RPD.2004.10.1.025
Over 98％ of Dendranthema x grandiflorum plants growing under a glasshouse in Gumi, Korea were infested with Meloidogyne hapla. Dendranthema x grandiflorum cv, `Ardilo` had fewer eggmass (3.3/g root), followed by cv. `Euro` (6.5), and cv. `Monarisa` had the most eggmasses(17). The coefficient correlation between plant growth and nematode reproduction(number of eggmass per plant) indicated that nematode reduced chlorophyll content in leaf (r
Detection of Rice Stripe Virus using RT-PCR
Lee, Bong-Choon ; Hong, Yeon-Kyu ; Kwak, Do-Yeon ; Oh, Byeong-Geun ; Park, Sung-Tae ; Kim, Soon-Chul ;
Research in Plant Disease, volume 10, issue 1, 2004, Pages 30~33
DOI : 10.5423/RPD.2004.10.1.030
Until now, occurrence of rice stripe virus (RSV) is limitted in southern part of Korea. However, recently the occurrence of RSV is increased and spreaded in central part of Korea including Chungcheong and Kyonggi province. It is very difficult to distinguish RSV symptoms on virus symptom physiological damage of rice. We detected RSV viral RNA from infected rice and its insect vector Laodelphax striatellus using specific primer of RSV-polymerase and coat protein gene with reverse transcription (RT)-PCR. The result of RT-PCR, we observed specific band including RSV-polymerase (1,,023 bp) and CP (969 bp) in both host of rice and insect vector.
Growth Damage and Alteration of Cellular Tissue of Barley Infected by Barley yellow mosaic virus
Park, Jong-Chul ; Lee, Jae-Dong ; Seo, Jae-Hwan ; Kim, Yang-Kil ; Jeong, Seon-Gi ; Kim, Hyung-Moo ;
Research in Plant Disease, volume 10, issue 1, 2004, Pages 34~38
DOI : 10.5423/RPD.2004.10.1.034
The damage of plant growth and alteration of cellular tissues of barley infected by Barley yellow mosaic virus (BaYMV) was explored. The infected plots significantly damaged in all of measured factors by the disease. In severely diseased plant, the viral infection affected on plant growth like as shorten culm length about 25cm, 36％ constrained ratio, comparing to healthy. The yield decreased over 70％ in diseased plots by fewer numbers of spike and kernel per square meter and spike, respectively. BaYMV constructed typical inclusion body like a pinwheel type inside barley leaves, and the infection affected on cellular elongation or growth not cell division in examined three parts as stem, neck of panicle and node, related to dwarfness of infected barley. The stem tissues were most severely affected on cell growth as restrained epidermis cell length in diameter and vascular bundle size. In neck of panicle tissues, distribution and size of tissues of fiber and cortex parts, respectively, showed differences between healthy and infected plants. In node part, healthy plant showed bigger tissue size as 1.5 times than infected plant. Theses results suggest that BaYMV infection could affect on the cell growth not cell division, and which resulted shorten culm length in plant growth and decreased yield, finally.
Virus Diseases Occurred on Watermelon in Jeonnam Province
Ko, Sug-Ju ; Lee, Yong-Hwan ; Cha, Kwang-Hong ; Park, Jin-Woo ; Lee, Su-Heon ; Yang, Kwang-Yeol ;
Research in Plant Disease, volume 10, issue 1, 2004, Pages 39~43
DOI : 10.5423/RPD.2004.10.1.039
Recent occurrence of virus diseases on watermelon plants cultivated in Jeonnam province was investigated from 1998 to 2002. While virus diseases were severely occurred on watermelon cultivated in green house in 1998, those of open field were severer than in green house since 2000. When 128 samples collected from different fields were examined by electron microscopy, 87.8％ of the samples contained rod-shaped or filamentous virus particles. RT-PCR analysis of the samples revealed that Cucumber green mottle mosaic virus (CGMMV) was only detected from collected samples at May. Watermelon mosaic virus (WMV) was most frequently found and CGMMV and Zucchini yellow mosaic virus (ZYMV) were slightly at June and July. However Cucumber mosaic virus (CMV) and Papaya ringspot virus (PRSV) have not been detected.
Soil Transmission of Cucumber green mottle mosaic virus and Its Control Mensures in Watermelon
Choi, Gug-Seoun ; Kim, Jae-Hyun ; Kim, Jeong-Soo ;
Research in Plant Disease, volume 10, issue 1, 2004, Pages 44~47
DOI : 10.5423/RPD.2004.10.1.044
Soil transmission ratio of Cucumber green mottle mosaic virus (CGMMV) was 0.2 to 3.5 ％ in watermelon growing fields naturally infested with the virus. Biological activities of CGMMV lost after 17 months in moist well-aerated soil but still continued more than 33 months in waterlogged soil. To inhibit the virus infection through soil, the roots of watermelon seedlings were soaked in 10％ solution of skim milk prior to transplanting. The seedlings treated with skim milk solution were not infected, while 5.0 to 7.6％ out of control seedlings were infected. The roots treated with skim milk were coated with membrane around the roots under scanning electron microscope.
Influence of CGMMV Infection Times on Growth and Quality of Watermelon and Cucumber
Ko, Sug-Ju ; Lee, Yong-Hwan ; Lee, Tae-Seon ; Yang, Kwang-Yeol ; Park, Jin-Woo ; Choi, Hong-Soo ;
Research in Plant Disease, volume 10, issue 1, 2004, Pages 48~52
DOI : 10.5423/RPD.2004.10.1.048
We investigated the effect of infection time of CGMMV on the growth and quality of watermelon and cucumber plants. The effect (damages by CGMMV) was estimated on the watermelon where CGMMV had been inoculated at different growth stages, vegetative (transplanting stage, vegetative growth stage) and reproductive growth stage (fruiting stage and fruit hypertrophy stage). In the case of cucumber, CGMMV was inoculated at transplanting stage and Erst flowering stage, respectively. When watermelon was infected with CGMMV at vegetative growth stage, vine length, internode length, leaf area, and fruit weight of the plants largely decreased compared with control plants, while the infected plant growth was not very different from control plants when it was infected at reproductive growth stage. Brix of the fruit of watermelon also decreased when the plants was infected with the virus earlier than fruiting stage. The occurrence of `Pisubag`, internal discoloration and decomposition of watermelon fruits, tended to be increased as earlier infection time with CGMMV In the case of cucumber infection time with CGMMV did not influence earlier growth of the plants, but did later growth showing that plant height, vine length, internode length, number of leaf, leaf wide, and leaf length of the plants decreased as infection time became to be earlier.
Detection of Cucumber green mottle mosaic virus in Bottle Gourd Seeds by RT-PCR
Lee, Sook-Kyung ; Song, Wan-Yeob ; Kim, Hyung-Moo ;
Research in Plant Disease, volume 10, issue 1, 2004, Pages 53~57
DOI : 10.5423/RPD.2004.10.1.053
Cucumber green mottle mosaic virus (CGMMV) was a major pathogen of watermelon and had affected seriously to watermelon production in Korea. Rapid and sensitive detection method of CGMMV associated with bottle gourd (Lagenafia siceraria) seeds was developed by using RT-PCR in this study. A pair of primeri Wmfl and Wmrl, specific for CGMMV was designed from coat protein gene sequences of CGMMV-W and used for amplifying 420 bp product in RT-PCR. To simplify the virus extraction procedure and reduce an inhibitor from the extract for the RT-PCR, some methods using ethanol precipitation, double filtration, polyethylene glycol precipitation and phenol/chloroform/isoamyl alcohol extraction procedure were compared and the phenol/chloroform/isoamyl alcohol extraction procedure was selected by its enhanced sensitivity. This detection method using the selected extraction step and the primers for RT-PCR could reliably detect an infected level of one CGMMV-infested seed in 1,000 seeds. This rapid and sensitive RT-PCR assay provides auseful tool for the specific detection of CGMMV in bottle gourd seed samples containing high levels of back-ground inhibitors.
Contamination Level of Ralstonia solanacearum in Soil of Greenhouses Cultivating tomato Plants in Chungbuk Province and Characteristics of the Isolates
Yun, Gon-Sig ; Park, Sang-Yong ; Kang, Hyo-Jung ; Lee, Ki-Yeol ; Cha, Jae-Soon ;
Research in Plant Disease, volume 10, issue 1, 2004, Pages 58~62
DOI : 10.5423/RPD.2004.10.1.058
Contamination level and characteristics of Ralstonia solanacearum in soil of greenhouses cultivating tomato plants in Chungbuk province was determined. R. solanacearum was isolated with the semiselective media in 27 greenhouse soil samples out of 133 greenhouses soil investigated, which indicates 20.3 ％ of tomato cultivating greenhouses in Chungbuk contaminated with the bacterial wilt pathogen. Density of R. solanacearum was estimated to 10
cfu/g in the contaminated soil. All 71 isolates of R. solanacearum which containing 12 isolates from the diseased tomato plants were race 1, ann 35 isolates of them were biovar 3 and 36 isolates were biovar 4. More than 88％ of 71 isolates were inhibited growth on nutrient agar containing oxolinic acid 0.5
/ml, streptomycin 25
/ml, tetracycline 5
/ml and cupric sulfate 375
/ml (1.5 mM). The 11.3％, 4.2％ and 5.6 ％ of the isolates can grow on nutrient agar containing 10 times more oxolinic acid, streptomycin, tetracycline than minimal inhibitory concentration of the sensitive strains. Five isolates were resistant to 2 bactericides and one isolates was resistant to all 3 bactericides.
Isolation and Identification of Rice Root Endophytic Antagonistic Serratia marcescens
Lee, Sook-Kyung ; Song, Wan-Yeob ; Kim, Hyung-Moo ;
Research in Plant Disease, volume 10, issue 1, 2004, Pages 63~68
DOI : 10.5423/RPD.2004.10.1.063
Twenty-three strains of Serratia sp., isolated from surface-sterilized rice roots collected in Chonbuk and Chungnam province, were identified and characterized. They were Gram-negative, rod shaped and red pigmented typically and their endophytism was confirmed by inoculation and reisolation of the strains in planta. Their antifungal activity against 4 rice pathogenic fungi was compared and ranged from 62.4 to 85.2％ against Rhizoctonia solani and 68.0 to 88.5％ against Pyricularia grisea. Among the 23 strains tested, strain Rsm220 showed the strongest inhibition activity against 4 pathogenic fungi. The strain was, therefore, selected as a biocontrol candidate for both the pathogens and its bacteriological characteristics and 165 rDNA sequences were analyzed. Phenotypic and biochemical characteristics of the selected Rsm220 were highly related to the type strain of S. marcescens and 165 rDNA sequencing of Rsm220 showed a homology of 98.2％ to the type strain of S. marcescens. The strain Rsm220 was identified as S. marcescens and the inhibition result of this endophytic strain indicates that it is a potential biocontrol agent for R. solani and R grisea.
The Effect of Fungicides against Rice Blast by the Nursery Treatment at Rice Seedling
Kang, Beum-Kwan ; Min, Ji-Young ; Kim, Yun-Sik ; Van Bach, Nguyen ; Jung, Hae-Yeon ; Cho, In-Joon ; Kim, Heung-Tae ;
Research in Plant Disease, volume 10, issue 1, 2004, Pages 69~72
DOI : 10.5423/RPD.2004.10.1.069
The control activity of isoprothiolane and tricyclazole mixed with carbosulfan, and probenazole by the nursery treatment was performed against rice leaf and neck blast caused by Magnaporthe grisea. In the paddy field, three fungicides showed good activities against leaf blast 3 months after nursery treatment. Especially the activity of tricyclazole against leaf blast gradually increased by the laps of time to 85.5％, which was assessed at 6 September,2003. Although the control value of isoprotholane and tricyclazole mixed with carbosulfan against neck blast was 47.5％ and 61.1％, respectively, probenazole showed a very high activity against not only leaf blast but also neck blast, of which that was 91.2％. No phytotoxicity was observed in all the treatments after transplanting rice seedling in the paddy field. Based on these results, three systemic fungicides tested in this study showed such a good potential that they might be used to formulate the nursery treating granule.
Gene Analysis of Resistance to Bacterial Blight, Xanthomonas oryzae pv, oryzae in Korean Six Rice Cultivars
Ryuk, Jin-Ah ; Choi, Chun-Hwan ; Kang, Hee-Kyoung ; Choi, Jae-Eul ;
Research in Plant Disease, volume 10, issue 1, 2004, Pages 73~77
DOI : 10.5423/RPD.2004.10.1.073
The gene analysis of resistance in rice cultivars, Daeanbyeo, Hwasunchalbyeo, Daejinbyeo, Naepungbyeo, Hwajinbyeo and Surabyeo to strains of Xanthomonas oryzae pv. oryzae was studied. F
plants and F
populations from the crosses between six cultivars and near isogenic lines carrying the single bacterial blight(BB) resistance gene were analyzed using Korean and Japanese BB races. Daeanbyeo, Hwasunchalbyeo, Daejinbyeo, Naepungbyeo, Hwajinbyeo and Surabyeo are alleic with IRBB101 but are non-alleic with IRBB104 and IRBB105. The allelic tests indicated that Daeanbyeo, Hwasunchalbyeo, Daejinbyeo, Naepungbyeo, Hwajinbyeo and Surabyeo have the Xal gene for resistance.
Disease Resistance Test Method of Cucumber Powdery Mildew(Sphaerotheca fusca) Using A Leaf Disk Assay
Lee, Yong-Hwan ; Seo, Jong-Bun ; Choi, Kyong-Ju ; Park, In-Jin ; Yang, Won-Mo ;
Research in Plant Disease, volume 10, issue 1, 2004, Pages 78~81
DOI : 10.5423/RPD.2004.10.1.078
The resistance of 10 varieties of cucumber (Cucumis sativus L.) to powdery mildew, caused by Sphaerotheca fusca, was evaluated by a leaf disk assay. Leaf disks (10 mm in diameter) were removed from fully expanded leaves and then placed in petri dishes containing 0.16％ water agar amended with benzimidazole. Leaf disks were inoculated by dropping a 10
l of conidia suspension. Conidiophore formation of powdery mildew was the greatest at
. The response of the host to powdery mildew, based on the inoculation onto disks of the first leaf, highly correlated with results obtained from harvesting stage of cucumber plants in greenhouse test (r
Suppression of Bacterial Soft Rot on Chinese Cabbage by Calcium Fertilizer Treatment
Kim, Byung-Sup ; Yeoung, Young-Rog ;
Research in Plant Disease, volume 10, issue 1, 2004, Pages 82~85
DOI : 10.5423/RPD.2004.10.1.082
Bacterial soft rot by Erwinia carotovora subsp. carotovora is one of the diseases causing the biggest damages in Chinese cabbage cultivation. This study was conducted to evaluate on suppressive effect of calcium fertilizer to bacterial soft rot of Chinese cabbage. Seven calcium fertilizers were selected for evaluation. And screening was conducted to select effective agents for controlling bacterial soft rot. When applied by the nursery test condition using mineral oil inoculation method with Chinese cabbage, calcium hydroxide had more suppressive efficacy than any other calcium fertilizer, While nitrogen fertilizer was induced the disease, calcium hydroxide was suppressed soft rot disease in field test as well as seedling test. Treatment of calcium+nitrogen fertilizer as well as calcium only showed a significant control effect in the field experiment with Chinese cabbage `Sanchon` in 2003.