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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Research in Plant Disease
Journal Basic Information
Journal DOI :
Korean Society of Plant Pathology
Editor in Chief :
Volume & Issues
Volume 12, Issue 3 - Dec 2006
Volume 12, Issue 2 - Aug 2006
Volume 12, Issue 1 - Apr 2006
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Potato Pests Observed in Seed Potatoes, North Korea during 2001 to 2005
Hahm Young-Il ;
Research in Plant Disease, volume 12, issue 1, 2006, Pages 1~4
DOI : 10.5423/RPD.2006.12.1.001
During visiting North Korea from 2001 to 2005, I have had a few chances to observe and discuss several North Korean scientists for the seed production program and also, the occurrence of potato pests. Healthy seed production, especially in the early generations, e.g. the production of virus-free starting materials as well as in vitro pre-basic seeds (G0) by hydroponics and basic seeds under netted houses according to her new national seed potato program of Academy of Agricultural Science, Pyongyang, North Korea, has been done well so far. Some major pests occurred, however, in the early generations such as pre-basic seed (G0) in greenhouse, basic seed (G1) in screenhouse, foundation seed-I (G2) and even ware potatoes in the fields are Phytopitthora infestans, Spongospora subterrunea, Ralstonia solanacearum, Pythium spp. and some viruses such as Potato virus X, Potato virus Y, Potato leafroll virus, and also larger potato ladybeetle, greenhouse whitefly and potato tuber moth. Therefore, the success of healthy seed production in North Korea will be thoroughly depended on the pest control and the multiplication of virus-free seed stocks in the isolated areas, especially where no infected potatoes are grown.
Effects of Development Time and Severity of Rice Ear Blight on Rice Yield and Quality
Shim Hong-Sik ; Hong Sung-Jun ; Hong Sung-Gi ; Kim Yong-Ki ; Ye Wan-Hae ; Sung Jae-Mo ;
Research in Plant Disease, volume 12, issue 1, 2006, Pages 5~9
DOI : 10.5423/RPD.2006.12.1.005
Grain discoloration, caused by several microorganisms, is a minor disease, but it is gaining importance in almost all rice growing areas of the world in recent years. Such grains are of low market value and consumption appeal in addition to their abnormal performance as seed of source of nutrient value. When the casual agents were inoculated on rice plant,`Ilmi-byeo`(at right before heading time and on heading time), the incidence of rice ear blight caused by Alternaria alternate and Nigrospora oryzae was higher in a plot inoculated at right before heading time than (in n plot inoculated) on heading time. Meanwhile, the incidence of rice ear blight caused by Bipolaris oryzae and Curvularia lunate was higher in a plot inoculated on heading time than in a plot inoculated at right before heading time. In rice quality, the ratio of imperfect grains was increased at right before heading time or on heading time by Nigrospora oryzae or Alternaria alternata, respectively. As discoloration rate of rice grains increased on `Ilmi-byeo`, the percentage of perfect grains decreased proportionally and the percentage of damaged rice grains increased remarkably.
Effect of Temperature on Pathogen Growth and Damage Analysis of Leaf Spot Disease on Grapevine Caused by Paeudocercospora vitis in Korea
Park Jong-Han ; Han Kyeong-Suk ; Lee Jung-Sup ; Seo Sang-Tae ; Jang Han-Ik ; Kim Heung-Tae ;
Research in Plant Disease, volume 12, issue 1, 2006, Pages 10~14
DOI : 10.5423/RPD.2006.12.1.010
This study was carried out to know damages of the grapevine trees by the disease to the leaf spot from 2000 to 2003. The isolates collected from different varieties and locations were identified as Pseudocercospora vitis (
.) Speg. based on the morphological and cultural characteristics. According to the in vitro test, the range of temperatures for the mycerial growth and the conidical germination of the fungus were from
and optimum temperature was
. There were remarkably different features between a low infection trees group and high infection trees group in terms of number of leaves per fruit branch, length and diameter of internode and leaf area.
Pathogenicity and Infection Mechanism of Pseudocercospora vitis Causing Leaf Spot Disease on Grapevine in Korea
Park Jong-Han ; Han Kyeong-Suk ; Lee Jung-Sup ; Seo Sang-Tae ; Jang Han-Ik ; Kim Heung-Tae ;
Research in Plant Disease, volume 12, issue 1, 2006, Pages 15~19
DOI : 10.5423/RPD.2006.12.1.015
This study was carried out to know pathogenicity of the pathogen isolated in several location of Korea and penetration mechanism of the Pseudocercospora vitis (
.) Speg. Inoculation tests at room temperature were performed on both sides of leaves with different isolates of the fungus. The typical symptoms appeared on the abaxial leaf surface, but no symptom was observed on the adaxial leaf surface with all isolates. The average incubation period was nine days, since all symptoms appeared from 8 to 10 days after inoculation. In order to know the mechanism of invasion of P. vitis to grapevine, the behavior of penetration hyphae through stomata were compared in two cultivars having different level of resistance. In order to know the mechanism of resistance of grape vine to P.vitis, two cultivars having different level of resistance were compared by counting the numbers and measuring size of the stomata per leaf. In a susceptible cultivar Campbell Early, the number of stomata was more than that of a resistance cultivar `Kyoho`. In a susceptible cultivar `Campbell Early`, the fungus entered readily into stomata after inoculation. However, in a resistant cultivar `Kyoho`, the fungus seemed to pass over or surrounded only the guard cells. In comparison of height of guard cells of stomata between the two cultivars, significant differences were observed by scanning electron microscopy and light microscopy. The height of guard cells of `Campbell Early` showed a little higher than those of `Kyoho` known to be resistant to the fungus.
Identification of Pathogens Associated with Bulb Rot of Lily during Storage and Effects of Bulb Disinfection on Development of Lily Bulb Rot
Hahm Soo-Sang ; Oh So-Young ; Lee Eun-Mo ; Yu Seung-Hun ;
Research in Plant Disease, volume 12, issue 1, 2006, Pages 20~24
DOI : 10.5423/RPD.2006.12.1.020
Several pathogens associated with bulb rot of lilies in storage house were identified with respect to rot types. Rot patterns were grouped into four different types; brown rot of bottoms, brown rot of shoots, water-soaked rot and blue mold. Brown rot of bottoms was the highest in frequency with 72.5%, and brown rot of shoots the least with 23.0%. Dominant pathogens were differed with rot patterns, brown rot of bottoms by Fusarium oxysporum, blue mold and brown rot of shoots by Penicillium brevicompactum and P. fellutanum. In wound-inoculation tests, Penicillium and Fusarium isolates caused severe rot on the bulbs. Bulb disinfection before storage by captan showed the most prominent control value of 95.2% followed by thiophanate-methyl with 85.6%.
In Situ Localization of Rice dwarf phytoreovirus P12 Protein in Infected Rice Plant
Lee Bong-Choon ; Hong Yeon-Kyu ; Hong Sung-Jun ; Park Sung-Tae ;
Research in Plant Disease, volume 12, issue 1, 2006, Pages 25~27
DOI : 10.5423/RPD.2006.12.1.025
Rice dwarf phytoreovirus (RDV), a member of the family Reoviridae has a genome composed of 12 segmented dsRNAs designated as 51 to 512 with an increasing order of mobility in polyacrylamide gel electrophoresis (PAGE). RDV encode 12 structural and non-structural proteins,
which are encoded by the
segments of the dsRNA genome, respectively. In this experiment, we confirmed in situ localization of RDV particles and P12 in cytoplasm of infected rice plant. We observed specific reaction of the gold particles using virus particle and P12 protein specific antiserum with protein A-gold immunolabelling in electron microscope. It was observed that gold particles specifically react to virus particles in cytoplasm in case using the antiserum for virus particles. In the case of antiserum for P12 protein, gold particles sporadically existing on cytoplasm without existing in organelle of cytoplasm specifically. As this result, RDV P12 protein encoded by S12 located in cytoplasm.
Seasonal Incidence of Potato virus f Infection on Potato Cultivars for the Double Crops in Korea
Hahm Young-Il ; Lee Young-Gyu ;
Research in Plant Disease, volume 12, issue 1, 2006, Pages 28~31
DOI : 10.5423/RPD.2006.12.1.028
One of major potato viruses is Potato virus Y (PVY) in Korea. In the southern part of Korea, potatoes have been grown as double crops in a year by using cv. `Dejima` and `Chubak` due to very short dormancy. However, they have caused a serious problem such as a rapid degeneration. It has been thought that the degeneration is affected by the high incidence of PVY in neighboring potato fields. Therefore, the investigation of factors causing the degeneration is very important in the production of healthy seed potato. In this study, the PVY reinfection rates of several potato varieties and the different seed sources of cv. `Chubak` have been investigated. Results show that the lowest infection rate of PVY among four potato cultivars derived from minitubers is cv. `Superior`. The others are in order of `Dejima`, `Atlantic` and `Chubak`. Also, the incidences of PVY differ significantly when several seed sources are examined. When the seed potatoes (G2, the progeny of microtuber) as spring potato crops are planted in area without potato field nearby, the infection rate of PVY is as low as that of microtubers. However, PVY incidence in the progenies of minitubers as fall potato crops largely increases. Therefore, the best way of potato production under double cropping system is to use the healthy seed potato produced in area without potato field and plant relatively resistant cultivar such as Dejima.
Response to Fungicides of Colletotrichum Species Isolated from Infected Tissues of Several Crops
Kim Joon-Tae ; Min Ji-Young ; Kim Heung-Tae ;
Research in Plant Disease, volume 12, issue 1, 2006, Pages 32~39
DOI : 10.5423/RPD.2006.12.1.032
This study has been conducted to investigate the responses of various isolates of four Colletotrichum species such as C. gloeosporioides, C acutatum, C. coccodes, and C. dematium isolated from infected tissues of several crops to fungicides such as carbendazim, carbendazim+diethofencarb, four protective fungicides, and three ergosterol biosynthesis-inhibiting (EBI) fungicides. All the isolates of C. acutatum showed
values in a range of 0.001-3.040
against carbandazim, a benzimidazole fungicide. As for the response to carbendazim, the isolates of C. gloeosporioides obtained from pepper, apple, and strawberry were clearly divided into two groups, resistant or sensitive isolates. All the resistant isolates showed
values above 1000
, whereas the sensitive isolates had lower
values than 0.550
. The isolates of C. gloeosporioides exhibited a negative cross resistance between carbendazim and diethofencarb (a N-phenylcarbamate fungicide), but isolates of C. acutatum did not. Toward carbendazim, C. coccodes and C. dematium isolates showed a similar response to C. acutatum isolates and the sensitive isolates of C. gioeosporioides, respectively. As for response of Protective fungicides, all the isolates of C. acutatum showed a more resistant reaction than all the isolates of C. gloeosporioides. However, there was no difference among 4 species of Colletotrichum against EBI fungicides.
Induction of Disease Resistance by Acibenzolar-S-methyl, the Plant Activator against Gray Mold (Botrytis cinerea) in Tomato Seedlings
Lee Jung-Sup ; Kang Nam-Jun ; Seo Sang-Tae ; Han Kyoung-Suk ; Park Jong-Han ; Jang Han-Ik ;
Research in Plant Disease, volume 12, issue 1, 2006, Pages 40~45
DOI : 10.5423/RPD.2006.12.1.040
The plant defence activator, Acibenzolar-S-methyl [benzo (1,2,3) thiadiazole-7-carbothioic acid-S-methyl ester, ASM] was assayed on tomato seedlings for its ability to induce resistance against Botrytis cinerea, the causal agent of gray mold in tomato. Pre-treatment of plants with ASM reduced the severity of the disease as well as the growth of the mycelium in plants. In ASM treated plants, reduction in disease severity (up to 55%) was correlated with suppression of mycelia growth (up to 46.5%) during the time course of infection. In plants treated with ASM, activities of peroxidase were determined as markers of resistance. Applications of ASM induced Progressive and significant increase of the enzyme in locally treated tissues. Such responses were expressed earlier and with a much higher magnitude when ASM-treated seedlings were challenged with the pathogen, thus providing support to the concept that a signal produced by the pathogen is essential for triggering enhanced synthesis and accumulation of the enzymes. No such activities were observed in water-treated control plants. Therefore, the slower symptom development and reduction in mycelium growth in ASM treated plants might be due to the increase in activity of oxidative and antioxidative protection systems in plants.
Effects of Streptomyces griseofuscus 200401 on Growth of Pepper Plants and Phytophthora Blight by Phytophthora capsici
Lim Tae-Heon ; Kwon Soon-Youl ; Kim Jin-Ho ;
Research in Plant Disease, volume 12, issue 1, 2006, Pages 46~50
DOI : 10.5423/RPD.2006.12.1.046
The microorganisms with the antifungal activity against Phytophthora capsici and Colletotrichum acutatum and the plant growth-promoting activity were screened from a forest and natural fields of Gajang-Dong, Sangju-city. One of the isolates, Streptomyces griseofuscus 200401, was selected as a good plant growth-promoting strain in this study. In greenhouse test, the number of leaf, fresh weight, and dry weight of pepper plants, that were grown with treatment of culture suspension or powder containing S. griseofuscus 200401, were higher than those without the bacterial cells. Cultivation of S. griseofuscus 200401 strain for 7 days in a nutrient rich medium produced ammonium chloride up to 0.13
in the culture solution of S. griseofuscus. Treatment of the selected strain significantly reduced the severity of the late blight of pepper plants to show the equivalent disease control activity to chemical fungicide. This study suggests that S. griseofuscus 200401 strain could be a potential biological agent with the biocontrol activity and the plant growth-promoting activity.
Control of Powdery Mildew by Garlic Oil in Cucumber and Tomato
Seo Sang-Tae ; Lee Jung-Sup ; Park Jong-Han ; Han Kyoung-Suk ; Jang Han-Ik ;
Research in Plant Disease, volume 12, issue 1, 2006, Pages 51~54
DOI : 10.5423/RPD.2006.12.1.051
The effectiveness of four plant oils (garlic, ginger, cinnamon and lemongrass) against a range of plant pathogenic organisms was tested in vitro. Of the four oils, two oils (garlic and cinnamon) showed relatively good antimicrobial activities. Activity of garlic oil was shown against the plant pathogenic bacteria Agrobacterium tumefaciens, Ewinia carotovora, Ralstonia solanacearum, Xanthomonas campestris, and the fungi Phytophthora infestans, Fusarium oxysporum, Collectotricum sp., whereas Acidovorax avenae and Pythium sp. showed the resistance towards garlic oil. Results from the planta bioassays under greenhouse conditions indicated that garlic oil significantly reduced the cucumber powdery mildew (Sphaerotheca fusca) and tomato powdery mildew (Erysiphe cichoracearum) showing control value 70.0-74.6% and 71.2%, respectively.
Occurrence of Stem Rot of Houttuynia cordata Thunb. Caused by Sclerotium rolfsii
Park Kyeng-Seok ; Park Jun-Hong ; Kim San-Young ; Choi Jang-Soo ;
Research in Plant Disease, volume 12, issue 1, 2006, Pages 55~57
DOI : 10.5423/RPD.2006.12.1.055
Stem rot of Houttuynia cordata occurred in the experiment field of Research Institute of Natural Product, Uiseong, Gyeongbuk, Korea in July 2003. The typical symptoms of the disease were water-soaking, stem rot, necrotic tuber. White mycelial mats were spread over lesions and sclerotia were formed on died plant and near soil line. The sclerotia were spherical and irregular, 1-2.5 mm on potato dextrose agar (PDA), white to brown in color, The optimal temperature for hyphal growth was
and the hyphal diameter was
. Clamp connections were observed on PDA. Pathogenicity of the casual organism was provided on H. cordata according to Koch`s postulate. The fungus was identified as Sclerotium rolfsii Saccardo on the basis of mycological characteristics and pathogenicity test to host plants. This is the first report on the stem rot of H. cordata caused by S. rolfsii in Korea.
Occurrence of Gray Mold on Balsam Pear (Momordica charantia) Caused by Botrytis cinerea in Korea
Kwon Jin-Hyeuk ; Park Chang-Seuk ;
Research in Plant Disease, volume 12, issue 1, 2006, Pages 58~61
DOI : 10.5423/RPD.2006.12.1.058
Ggray mold caused by Botrytis cinerea occurred on Balsam pear (Momordica charantia) at Daesan-myon, Changwon city, Gyeongnam province in Korea from 2004 to 2006. The disease symptoms usually started with water-soaking lesions on the fruits tip and stem, and then the infected plants became withered and eventually died. The conidia of the pathogen appeared on the surface of fruit and stem of infected plants. The conidia were one celled and mostly ellipsoid or ovoid in shape and were light gray in color, The conidia were
in size and conidiophores were
in length. The sclerotia formed abundantly on potato-dextrose agar 18 days after incubation. The optimum temperature for sclerotial formation was
. Pathogenicity of the causal organism was proved according to Koch,s postulates. The causal organism was identified as Botrytis cinerea Persoon: Fries based on the mycological characteristics. This is the first report on gray mold of M. charantia caused by B. cinerea in Korea.
Occurrence of Rice black-streaked dwarf fijivirus in Maize
Lee Bong-Choon ; Hong Yeon-Kyu ; Hong Sung-Jun ; Park Sung-Tae ;
Research in Plant Disease, volume 12, issue 1, 2006, Pages 62~64
DOI : 10.5423/RPD.2006.12.1.062
July in 2005, we collected infected maize plant that showing stripe dwarf disease on maize leaf in Jeonbuk provinces including Gochang-gun and conducted genomic dsRNA extraction and RT-PCR. Genomic dsRNA was extracted directly in infected maize plant and electrophoresis in agarose gel. We confirmed 10 segments of genomic dsRNA. We conducted RT-PCR by genomic dsRNA and specific primer of S7, S8 and S10. As a result, specific band of expected size was confirmed respectively. In the results of dsRNA and RT-PCR analysis, we confirmed Rice black-streaked dwarf fijivirus (RBSDV) from naturally infected maize plant. Occurrence of RBSDV of maize plant was dealt 22 ha`s damage in maize field. The occurrence rate was 80% in a lot of places of disease.