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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Research in Plant Disease
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Journal DOI :
Korean Society of Plant Pathology
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Volume & Issues
Volume 13, Issue 3 - Dec 2007
Volume 13, Issue 2 - Aug 2007
Volume 13, Issue 1 - Apr 2007
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Survey of Disease Occurrence in Tobacco Plants of the Kyeongbuk Area during 2005-2006
Yi, Young-Keun ; Yim, Young-Gu ;
Research in Plant Disease, volume 13, issue 1, 2007, Pages 1~5
DOI : 10.5423/RPD.2007.13.1.001
Major diseases of tobacco and farmer`s control practices were surveyed in the Kyeongbuk province area from 2005 to 2006. Mosaic caused by potato virus Y and bacterial wilt caused by Ralstonia solanacearum were most severe during the harvest season. Compared with the disease occurrence of ten years ago, the damage by tobacco mosaic virus reduced but bacterial wilt increased. These changes in the disease occurrences may probably be due to releasing the resistant tobacco cultivar to the mosaic virus but susceptible to the bacterial wilt pathogen. More than thirty percentage of the farmers have misused fungicides and also have applied the continuous mono-cropping system for more than ten years, and have chosen the incorrect crops for the rotation.
Root and Basal Stem Rot of Moth Orchid (Phalaenopsis spp.), Pung-nan (Neofinetia falcata) and Nadopung-nan (Aerides japonicum) Caused by Fusarium spp.
Kim, Jin-Won ; Chun, Se-Chul ;
Research in Plant Disease, volume 13, issue 1, 2007, Pages 6~14
DOI : 10.5423/RPD.2007.13.1.006
Root and basal stem rot disease occurred on moth orchid (Phalaenopsis spp.), Pung-nan (Neofinetia falcata) and Nadopung-nan (Aerides japonicum) grown in the farmers` greenhouses located in Namyangju Kyonggi province, Korea during 2005 to 2006. Wilting symptoms occurred on these orchard plants at initial stage and the infected plant leaves turned yellow to red. The discolored leaves were fallen down to lead to eventual death of the entire plant. A total of 59 isolates of Fusarium spp. was obtained from roots and leaf bases of the diseased plants. The cultural and morphological characteristics of isolated Fusairum spp. were identified as Fusarium oxysporum, F. proliferatum and F. solani. F. oxysporum and F. proliferatum were isolated from all these orchard plants but F. solani was isolated only from Phalaenopsis spp. Pathogenicity of the three Fusarium spp. was confirmed by artificial inoculation. Although F. oxysporum, F. proliferatum and F. solani cusing root rot disease in Phalaenopsis spp. have been reported in Korea, the pathogens in N. falcata and A. japonicum were not reported yet. Therefore, this is the first report on the root and stem rot of N. falcata and A. japonicum caused by F. oxysporum and F. proliferatum in Korea.
Occurrence and Pathogenicity of Verticillium Wilt on Chrysanthemum Caused by Verticillium dahliae
Han, Kyung-Sook ; Park, Jong-Han ; Lee, Jung-Sup ; Seo, Sang-Tae ; Cheong, Seung-Ryong ;
Research in Plant Disease, volume 13, issue 1, 2007, Pages 15~19
DOI : 10.5423/RPD.2007.13.1.015
A wilt disease on the greenhouse-grown chrysanthemum occurred at Kumi, Masan and Busan, Korea in
. Symptoms of wilting, yellowing, and marginal burning started from lower leaves and progressed upward. Early symptoms often appeared on one side, involving only one part of the plant or one side of individual leaves or stems without a vascular discoloration. Vascular tissues of the infected leaf-base`s discolored to brown. Fungal isolates obtained from discolored tissues were identified as Verticillium dahliae based on its cultural and morphological characteristics. The fungus showed whitish to creamy colony pattern with abundant dark brown to black elongated microsclerotia on PDA. Conidiophores were verticillately branched and conidia were ellipsoidal to sub-cylindrical shape, and measured as
. Pathogenicity tests by root dipping resulted in similar symptoms observed in the naturally infected chrysanthemum in fields.
Effects of Bacterial Leaf Blight Occurrence on Rice Yield and Grain Quality in Different Rice Growth Stage
Noh, Tae-Hwan ; Lee, Du-Ku ; Park, Jong-Chul ; Shim, Hyeong-Kwon ; Choi, Man-Yeong ; Kang, Mi-Hyung ; Kim, Jae-Duk ;
Research in Plant Disease, volume 13, issue 1, 2007, Pages 20~23
DOI : 10.5423/RPD.2007.13.1.020
We investigated the first symptom emerging date of rice bacterial leaf blight disease during four years from 2002 to 2005. The disease occurrence date was earlier 20
30 days in 2005 than that of 2002. The damage in different rice growth stage by the bacterial disease on rice yield and grain quality in southern part of Korea was examined. The disease decreased rice yield following by increased infection rates. Slight loss in rice yield and brown head rice rate were observed at below 10% level of infected leaf area, while over 25% infected leaf area caused the significant decrease in rice yield and brown head rice rate. More than 50% of the infected leaf area rate caused 29% yield reduction in case of infection at panicle formation stage and 18% of brown head rice rate. Cooked rice quality was also affected in the diseased rice by damage in palatability score and viscosity.
Genetic Diversity of Ralstonia solanacearum Strains Isolated from Pepper and Tomato Plants in Korea
Seo, Sang-Tae ; Park, Jong-Han ; Han, Kyoung-Suk ; Cheong, Seung-Ryong ; Lee, Seung-Don ;
Research in Plant Disease, volume 13, issue 1, 2007, Pages 24~29
DOI : 10.5423/RPD.2007.13.1.024
A total of 35 strains of Ralstonia solanacearum isolated from wilted pepper and tomato plants in Korea were analyzed for their genetic diversity by bacteriological, pathological and molecular biological approaches. All the strains were identified as R. solanacearum biovar 4 on the basis of physiological and biochemical tests, and species-specific PCR primers. Pathogenicity of the strains was confirmed by inoculating on 4-week-old pepper and tomato seedlings. Using cluster analysis based on repetitive sequence-based polymerase chain reaction (rep-PCR) genomic fingerprints, R. solanacearum strains isolated from pepper and tomato in Korea divided into 6 groups showing a high degree of genetic diversity at 55% similarity level. The genetic diversify of strains was not significantly correlated with their geographic origins and host plants.
Severity of Cowpea mosaic virus and Putkong Disease Monitoring and Purification of Cowpea mosaic virus
Cho, Eui-Kyoo ;
Research in Plant Disease, volume 13, issue 1, 2007, Pages 30~33
DOI : 10.5423/RPD.2007.13.1.030
One hundred and eighty-six leaves of soybean cv. Seokryangputkong that showed mild mosaic symptoms were collected randomly and ELISA tests were conducted with those leaf samples to screen the presence of Cowpea mosaic virus (CPMV). Ninety-three out of 186 samples reacted positively to CPMV, but those samples did negatively to Soybean mosaic virus (SMV). At least, 55 leaf samples revealed higher values than that of positive control. The results strongly confirmed that CPMV occurred severely in soybean cv. Seokryangputkong. However, a question is raised on the primary reservoir and vector for transmission of this virus. Since the farmer changes seeds every year, seed transmission is excluded. The virus was also purified, the analysis of coat protein conformed the virus of cowpea mosaic virus and UV absorption pattern confirmed that the causal virus of mosaic disease in soybean putkong was cowpea mosaic virus.
Effects of Lichen Colonization of Dirinaria applanata on the Death of Rhododendron schlippenbachii
Oh, Soon-Ok ; Koh, Young-Jin ; Hur, Jae-Seoun ;
Research in Plant Disease, volume 13, issue 1, 2007, Pages 34~39
DOI : 10.5423/RPD.2007.13.1.034
Lichen thalli of Dirinaria applanata heavily colonized on the twigs of dead or dying Rhododendron trees in Solok island, Jeonnam province in Korea. Pathogenesis of the lichen on the trees was investigated to find out the possibility of lichen as a causal agent. Histological examination of the lichen colonized twigs was attempted with differential staining technique. Lichen-forming fungus colonized only on the surface of bark and there was no direct penetration of fungal hyphae into the plant tissues. Symbiotic algae of the lichen was also examined. The isolated algal cells were inoculated on artificially induced wounds of the healthy trees. Histological examination of the inoculated tissues showed that some algal cells were successfully colonized inside the tissues without any pathogenic symptoms, even 2 months later, The extract of the lichen thalli was also examined using 10% of DMSO solution. Treated tissues showed no pathogenic symptoms, even 4 weeks later. The results suggested that the lichen was not directly involved in the death of the trees.
Control of Powdery Mildew of Pepper Using Culture Solutions of Chitinolytic Bacteria, Chromobacterium sp. and Lysobacter enzymogenes
Seo, Chong-Chan ; Jung, Hyun-Chae ; Park, Seur-Kee ;
Research in Plant Disease, volume 13, issue 1, 2007, Pages 40~44
DOI : 10.5423/RPD.2007.13.1.040
Powdery mildew of pepper is one of the most devastating diseases which is occurring all the year under greenhouse condition. In this study, control efficacy against powdery mildew was evaluated by mixed culture solutions of two chitinolytic bacteria, Lysobacter enzymogenenes strain C-3 and Chrornobacterium sp. strain C-61, cultivated in the chitin-supplemented medium. In all experiments, white powder on the reverse side of pepper leaves perfectly disappeared 3 days after application of mixed culture solutions. However, periods required for formation of new white powder on the same sites after application (control-lasting period) were largely differed according to environmental conditions. In particular, the control-lasting period was much longer when sprayed on 6 PM than 9 AM and especially, on rainy days than sunny days. This indicates that control efficacy of culture solution may be largely affected by environmental conditions after application. The undiluted culture solution resulted in a perfect control with control value more than 95% by application of 5-day-intervals under severely diseased field and 7-day-intervals under disease-started field. A ten-fold diluted product also showed control value more than 81% by application of the same method. These results suggest that this culture solution can be practically used to control powdery mildew disease in pepper plants.
Races and Dominant Population of Chinese Cabbage Clubroot Pathogen, Plasmodiophora brassicae in Korea
Jang, Se-Jeong ; Heo, Seung-Hwan ; Jang, Chang-Soon ; Kang, Sung-Woo ; Lim, Yong-Pyo ; Kim, Hong-Gi ;
Research in Plant Disease, volume 13, issue 1, 2007, Pages 45~49
DOI : 10.5423/RPD.2007.13.1.045
Single spores were isolated from infected roots of Chinese cabbage with a typical clubroot symptom, collected from different Chinese cabbage cultivation areas in Korea. When the single spore isolates were inoculated on Chinese cabbage, radish, turnip, kale, leaf mustard and Williams` differential varieties, among 321 roots harvested two weeks after inoculation, a visual symptom was observed on only one root and light/uncommon symptoms were done on 70 roots. These 71 individuals were homogenized and used as inocula. These inocula caused generally higher pathogenicity than that of single spore. Finally 15 isolates, with enough growth for conducting further experiment, were selected. These 15 individuals were grouped four, seven, two and two into race 1, race 4, race 9 and race 11, respectively, using Williams` differential set. It was confirmed that race 4 were dominantly present in Korea. These 15 had been obtained from roots of Chinese cabbages, radishes and turnips inoculated with single resting spores and had shown pathogenicity to Laurentian and Wilhelmsburger belong to Rutabaga in Williams` differential variety set. Therefore, we assume that such characteristic pathotypes including race 4, especially, of P. brassicae showing strong pathogenicity to Chinese cabbage, radish and turnip may be dominant in Korea.
Stem Rot of Disporum sessile Caused by Sclerotium rolfsii in Korea
Kwon, Jin-Hyeuk ; Shim, Chang-Ki ; Park, Chang-Seuk ;
Research in Plant Disease, volume 13, issue 1, 2007, Pages 50~52
DOI : 10.5423/RPD.2007.13.1.050
From 2004 to 2005, the stem rot of Disporum sessile D.
caused by Sclerotium rolfsii occurred spoyadieally in an herbs exhibition farm of Hamyang, Gyeongsangnam-do Agricultural Research and Extension Services, Korea. The typical symptom is water-soaking, dark brown on the stem and rotting, wilting. The infected plants were mostly died. White mycelial mats were spread over lesions, and then sclerotia were formed on stems and near soil line. The sclerotia were globoid in shape, 1
3 mm in size and white to brown in color, The optimum temperature and sclerotia on PDA was 30
and the hyphal width was
. The typical clamp connections were observed in the hyphae of the fungus grown on PDA. Pathogenicity of the causal organism was proved on D. sessile according to Koch`s postulate. On the basis of mycological characteristics and pathogenicity to host plants, this fungus was identified as Sclerotium rolfsii Saccardo. This is the first report on the stem rot of D. sessile caused by S. rolfsii in Korea.
Occurrence of Fruit Rot of Pumpkin Caused by Sclerotium rolfsii
Kwon, Jin-Hyeuk ; Shen, Shun-Shan ; Chae, Yun-Seok ; Park, Chang-Seuk ;
Research in Plant Disease, volume 13, issue 1, 2007, Pages 53~56
DOI : 10.5423/RPD.2007.13.1.053
The fruit rot of pumpkin caused by Sclerotium rolfsii occurred sporadically at Gyeongsangnam-do Agricultural Research and Extension Services, Korea in 2004 and 2005. The typical symptom of the disease included water-soaking, fruit rotting and wilting. White mycelial mats were spread over infected fruits and then sclerotia were formed on the lesions and near soil surface. The sclerotia were globoid in shape, 1
3 mm in size and white to brown in color. The optimum temperature for mycelial growth and sclerotia formation was at
on PDA and the hyphal width ranged from 4 to 8
. The typical clamp connections were observed in the hyphae of the fungus grown on PDA. On the basis of mycological characteristics and pathogenicity to host plants, this fungus was identified as Sclerotium rolfsii Saccardo. This is the first report on the fruit rot of Cucurbita spp. caused by S. rolfsii in Korea.
Rhizopus Soft Rot on Grape Caused by Rhizopus stolonifer in Korea
Kwon, Jin-Hyeuk ; Shim, Chang-Ki ; Chae, Yun-Seok ; Park, Chang-Seuk ;
Research in Plant Disease, volume 13, issue 1, 2007, Pages 57~60
DOI : 10.5423/RPD.2007.13.1.057
A rhizopus soft rot caused by Rhizopus stolonifer occurred sporadically on grape in orchard in Gyeongsang-nam-do Agricultural Research and Extension Services and in Wholesale Market of Agricultural Products in Jinju city. The infected fruits were rapidly water-soaked, softened and eventually rotted, and the symptoms were initiated from wounds or cracks occurred at harvest time. Sporangiophores were
in length and
in width. Sporangia were globose or hemispheric and
in size. The color of sporangia was white and cottony at first and gradually to brownish black, bearing abundant spores when it matured. Columella were hemispheric and
in size. Sporangiospores were irregular round or oval, brownish-black streaked and
in size. The optimum temperature for mycelial growth of the fungus on PDA was 25
. On the basis of mycological characteristics and pathogenicity to grape (Vitis vinifera), the causal fungus was identified as Rhizopus stolonifer (Ehrenberg ex. Fr,) Lind. This is the first report of Rhizopus soft rot by R. stolonifer on grape (V. vinifera) in Korea.
Bacterial Leaf Spot of English Ivy Caused by Xanthomonas hortorum pv. hederae
Lee, Seung-Don ; Lee, Jung-Hee ; Han, Kyoung-Suk ; Seo, Sang-Tae ; Kim, Yong-Ki ; Heu, Sung-Gi ; Ra, Dong-Soo ;
Research in Plant Disease, volume 13, issue 1, 2007, Pages 61~65
DOI : 10.5423/RPD.2007.13.1.061
English ivy (Hedera helix) in Araliaceae family is an evergreen climbing vine. A severe bacterial disease of English ivy was observed and investigated in January 2005. Initial symptoms on the leaves appeared as small water-soaked lesions on the lower surface. As the spots enlarged, the center became brown to brownish black and greenish-brown water-soaked, irregular margins surrounded the center, The spots developed into large irregular blotches, sometimes 5
10 mm in diameter, then coalesced. Finally, the water-soaked margins raised, dried out, became corky and broke in the center. A bacterial organism, isolated from the advancing margins of the lesions, was tested for its pathogenicity according to the Koch`s postulates and biochemical and physiological tests identified the isolated bacterium as a Xanthomonas. The representative Xanthomonas strains (SL4821 and SL4822) isolated from English ivy were compared with a reference strain X. hortorum pv. hederae for fatty acid profiles, metabolic fingerprints and 16s rDNA sequences, showing that all outcomes were indistinguishable between the representative and reference strains. This is the first report of bacterial leaf spot of English ivy in Korea.
Bacterial Leaf Spot and Dry Rot of Lettuce Caused by Xanthomonas campestris pv. vitians
Lee, Seung-Don ; Lee, Jung-Hee ; Kim, Yong-Ki ; Heu, Sung-Gi ; Ra, Dong-Soo ;
Research in Plant Disease, volume 13, issue 1, 2007, Pages 66~70
DOI : 10.5423/RPD.2007.13.1.066
During 1997 and 1998, a new disease of lettuce (Lactuca sativa) was observed on greenhouse-grown plants in Kwangju and Kwangmyung. Lesions on leaves were irregular, small, pale green to black, water-soaked, and 2 to 5 mm in diameter, Coalescing lesions sometimes caused defoliation of older leaves. Isolations made from diseased leaves on yeast extract dextrose calcium carbonate agar yielded nearly pure cultures of a yellow pigmented bacterium typical of a xanthomonad. Two bacterial strains (SL0246 and SL1352) were purified and used for further tests. Pathogenicity of strains was confirmed on 5-week-old lettuce plants injected with bacterial suspensions containing
cfu/ml of phosphate buffered saline. The representative Xanthomonas strains isolated from lettuce were compared with a reference strain X. campestris pv, vitians for fatty acid profiles and metabolic fingerprints using GN2 microplate, showing that all outcomes were indistinguishable between the representative and reference strains. This is the first report of bacterial leaf spot and dry rot of lettuce in Korea.
Virus Diseases Occurred on Squash in Jeonnam Province
Ko, Sug-Ju ; Lee, Yong-Hwan ; Cha, Kwang-Hong ; Lee, Su-Heon ; Choi, Hong-Soo ;
Research in Plant Disease, volume 13, issue 1, 2007, Pages 71~73
DOI : 10.5423/RPD.2007.13.1.071
Field disease incidences of squash virus diseases in Jeonnam province were estimated to be 76.1% and of delayed planting on August-September (retarding culture) and on February-March (semi-forcing culture) on glass house were 55.0% and 0%, respectively, in 2000. Disease incidences of individual squash plant within a field were 100% and 3.6%, respectively, in wild culture and retarding culture. Total of 61 samples suspected to be infected with viruses were collected in 2000 and tested by RT-PCR using specific oligonulceotide primer sets designed for the detection of Cucumber green mottle mosaic virus (CGMMV), Kyuri green mottle mosaic virus (KGMMV), Zucchini yellow mosaic virus (ZYMV), Papaya ring spot virus (PRSV), Watermelon mosaic virus (WMV), and Cucumber mosaic virus (CMV). Each specific primer set for WMV, ZYMV, and PRSV amplified expected size of DNA fragments from 16, 10, and 2 samples in wild culture, respectively. Double or triple infection were observed in 7 samples tested. In contrast, each specific primer set for WMV, ZYMV, and PRSV confirmed virus infection from 7, 6, and 6 samples, respectively, in samples collected from semi-forcing culture. Double infection of WMV and PRSV was observed in only one sample. However, no DNA fragment was amplified from RT-PCR using CGMMV, KGMMV, and CMV specific oligonucleotide primer sets indicating no CGMMV, KGMMV, or CMV infection in squash fields in Jeonnam province in 2000.