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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Research in Plant Disease
Journal Basic Information
Journal DOI :
Korean Society of Plant Pathology
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Volume & Issues
Volume 13, Issue 3 - Dec 2007
Volume 13, Issue 2 - Aug 2007
Volume 13, Issue 1 - Apr 2007
Selecting the target year
Aspects of Popcorn Disease Occurrence on Mulberry Fruits in Korea
Hong, Sung-Kee ; Kim, Wan-Gyu ; Sung, Gyoo-Byung ; Nam, Sung-Hee ; Kim, Jong-Sun ;
Research in Plant Disease, volume 13, issue 3, 2007, Pages 131~136
DOI : 10.5423/RPD.2007.13.3.131
Occurrence of popcorn disease on mulberry fruits was surveyed in several locations in Korea from 2005 to 2007. The diseased fruits turned grayish white and changed to hard and black sclerotia during overwintering after falling onto the ground. Apothecia produced from overwintered sclerotia in the fields of mulberry trees were observed in early May. Two types of apothecia were produced from the sclerotia, which were cup-shaped or club-shaped. The fungus with cup-shaped apothecia was identified as Ciboria shiraiana, and that with club-shaped apothecia as Scleromitrula shiraiana. C. shiraiana occurred at the ratio of about 6 vs. 4 in the fields. Occurrence of the disease differed remarkably depending on years, locations, and varieties of mulberry trees investigated. Field survey of the disease incidence showed that Baekhahyeongsang, Dahojosaeng, Hasusang, a native variety and Sawonppong 22 were resistant to the disease, Cheongilppong, Daeseongppong, Guksang 20 and Turkey-D were moderately susceptible, and Cateneo, Moreji and Sanggwa were highly susceptible. Disease incidence in the fields mulched with nonwoven fabrics or plastic films was much lower than that in the non-mulched fields. Disease incidence in the fields mulched with rice straws was similar to that in the non-mulched fields.
Genetic Diversity of Agrobacterium vitis Strains in Korea
Kim, Jong-Kun ; Choi, Jae-Eul ; Kang, Hee-Wan ;
Research in Plant Disease, volume 13, issue 3, 2007, Pages 137~144
DOI : 10.5423/RPD.2007.13.3.137
Fifty nine strains of Agrobacterium vitis, the causal agent of crown-gall disease on grapevine, originating from different geographical regions and 16 grapevine cultivars including 35 Kyoho cultivar of Korea, were characterized by PCR polymorphic analysis using Universal Rice Primer(URP). Of 12 URP primers, primers URP1F, URP2R, URP2F, and URP4R, URP17R were available for detecting PCR polymorphic bands among the A. vitis strains. PCR polymorphic bands produced by primers URP2F and URP17R were profiled to 12 strain types. A. vitis strains originated from Kyoho cultivar of grapevine showed relatively simple genetic diversify of the four PCR types, while the A. vitis strains originated from other grapevine cultivars and type culture strains showed various genetic diversity with 8 types. Unweighted Pair-Group Method with Arithmetic mean(UPGMA) cluster analysis using the URP-PCR polymorphic bands showed 59.4. vitis strains are genetically clustered into large seven groups.
Detection of Xanthomonas axonopodis pv. glycines and Survey on Seed Contamination in Soybean Seeds Using PCR Assay
Hong, Sung-Jun ; Hong, Yeon-Kyu ; Lee, Bong-Choon ; Lim, Mi-Jung ; Yoon, Young-Nam ; Hwang, Jae-Bok ; Song, Seok-Bo ; Park, Sung-Tae ;
Research in Plant Disease, volume 13, issue 3, 2007, Pages 145~151
DOI : 10.5423/RPD.2007.13.3.145
Xanthomonas axonopodis pv. glycines is the causal agent of bacterial pustule of soybean(Glycine max. (L.) Merr), which is one of the most prevalent bacterial diseases in Korea. In this study, Polymerase Chain Reaction (PCR) assay was applied to detect Xanthomonas axonopodis pv. glycines and to survey on seed contamination in 36 soybean cultivars of Korea. And we have to compare PCR assay with dilution-plating assay of detection and identification. We confirmed detection of pathogen from artificial infected seeds and natural Infected seeds using PCR assay. This assay gave results similar to a seed-wash dilution plating assay and proved more effective than classical methods. Results of survey on seed contamination by X. axonopodis pv. glycines from 36 cultivar seeds showed that the pathogen was detected from Pungsan-namulkong, Mallikong, Taekwangkong, Daemangkong, Ajukkarikong using PCR assay. Therefore, The PCR assay provides a sensitive, rapid tool for the specific detection of X. axonopodis pv. glycines in soybean seeds.
Control and Incidence of Leaf Blight on Lily with Different Cultural Systems
Hahm, Soo-Sang ; Lee, Ki-Hwan ; Lee, Jong-Won ; Lee, Hee-Duck ; Yu, Seung-Hun ;
Research in Plant Disease, volume 13, issue 3, 2007, Pages 152~156
DOI : 10.5423/RPD.2007.13.3.152
Incidences of leaf blight of lily cultivars Raizan and Casa Blanca in the open field cultivation were 50% and 45.4%, respectively, while those in the green house cultivation were significantly reduced to 1.5% and 1.9%, respectively, In the green house, the incidences of the disease in sprinkler watering cultivation were
, while those in drip watering cultivation were only
. Incidence of the disease was severe in the field where the lily was cultivated successively for 2 to 3 years. Isolation frequencies of B. elliptica from overwintered plant debrises such as leaves, stems, capsules, and bulbs were 43.3%, 46.7%, 60% and 0%, respectively, while those of B. cinerea were 10.3%, 0%, 3.3% and 0%, respectively, Incidence of leaf blight in the field where diseased plant debris was cleaned was 7.3%, while that in the field where diseased plant debris was not cleaned was 56.5%. Incidences of the disease in the field where coverages of soil surface with black vinyl, bark or rice straw were used were 6.6%, 8.2% and 11.3%, respectively, while that in the field where the coverage was not used was 21.3%.
Evaluation of Hot Water Treatment for Disinfection of Vegetable Seeds for Organic Farming
Lee, Ji-Hyun ; Shen, Shun-Shan ; Park, Yong-Ju ; Ryu, Kyung-Yul ; Jee, Hyeong-Jin ;
Research in Plant Disease, volume 13, issue 3, 2007, Pages 157~163
DOI : 10.5423/RPD.2007.13.3.157
Hot water treatment that is the most appropriate seed disinfection method for organic vegetable farming was evaluated in this study. Among the leafy vegetable seeds lettuce that was the most sensitive to hot water was suitable to treat at
for 25 min, while Chinese cabbage and radish seeds were optimally treated at
for 25 min. The treatments resulted in similar or higher seed germination rate than non-treated seeds and promoted plant growth. In addition, fungi such as Alternaria, Aspergillus, Penicillium, or Mucor grown on the seeds were suppressed over 90% and the bacterial growth on lettuce seeds reduced 98.5% by the treatment. Among the fruit vegetable seeds pumpkin that was vulnerable to hot water was suitable to treat at
for 15 min, while cucumber and hot pepper seeds revealed optimum treatment at
for 25 min as chinese cabbage and radish. The treatment also showed similar or higher seed germination rate and growth than non-treated seeds. Furthermore, fungi such as Rhizopus, Aspergillus, Penicillium or Mucor grown on the seeds reduced from 72.0% to 95.4%. The bacterial growth on cucumber and red pepper seeds was suppressed from 65.5% to 86.0% by the treatment. Results indicated that the hot water treatment is practical for disinfection of organic vegetable seeds and the optimum temperature and soaking time varied among the seeds.
Development of Control System with Fungicides against Diseases of Ginseng Plant
Kim, Joo-Hyung ; Lee, Seon-Wook ; Min, Ji-Young ; Bae, Young-Seok ; Shin, Myeong-Uk ; Kim, Sun-Bo ; Kim, Myoung-Ki ; Yeon, Cho-Rong ; Lim, Jin-Young ; Kim, Heung-Tae ;
Research in Plant Disease, volume 13, issue 3, 2007, Pages 164~169
DOI : 10.5423/RPD.2007.13.3.164
Three kinds of disease occurring on ginseng leaves, such as grey mold, Alternaria blight, and anthracnose, started at the beginning of June, July, and August, respectively. The disease incidence was rapidly increased from the beginning of rainy season. To develop the control system with fungicides, 6 fungicides were selected and applied on ginseng at the indicated time. Calculating the control value by using the area under the disease progressing curve (AUDPC), the control activities of the supervised control system with fungicides were 61.7, 78.8 and 70.5% against grey mold, Alternaria blight, and anthracnose, respectively. The application of the mixture of carbendazim and diethofencarb on first of June was very important in control system with fungicides. If it was deleted in control system, control value was decreased against grey mold. In the case of Alternaria blight and anthracnose, the application of difenoconazole on July 18, and trifloxystrobin on August 7 were indispensible. If difenoconazole and trifloxystrobin were not applied on July 18, and August 7, the control activities against Alternaria blight and anthracnose, respectively, were decreased to 28.9% and 44.4%.
Monitoring for the Resistance of Botrytis cinerea Causing Gingseng Gray Mold to Procymidone and Its Multiple resistance with the Mixture of Carbendazim/Diethofencarb
Lee, Seon-Wook ; Kim, Joo-Hyung ; Min, Ji-Young ; Bae, Young-Seok ; Kim, Heung-Tae ;
Research in Plant Disease, volume 13, issue 3, 2007, Pages 170~176
DOI : 10.5423/RPD.2007.13.3.170
Effects of fungicides on the mycelial growth of Botrytis cinerea isolated from ginseng leaves were investigated by an agar dilution method. By using a agar dilution method, it was investigated the effect of fungicides, procymidone, carbendazim and the mixture with both of carbendazim and diethofencarb, on the mycelial growth of Botrytis cinerea isolates, which were isolated from infected leaves of ginseng in 2005 and 2006. With MIC (minimum inhibiton concentration) of procymidone against B. cinerea, pathogens were divided into two groups. While one showed the low MIC between 0.8 and
, the other showed higher MIC above
. In terms of the inhibition ratio of mycelial growth at the indicated concentration of procymidone, isolates of B. cinerea were divided into three groups; the sensitive, the intermediate resistant, and the resistant group. Each group was differentiated by
; the sensitive group showed below
, the intermediate resistant group between 2.0 to
, and resistant group above
. Compared with the ratio of resistant isolates of B. cinerea in 2005, the ratio in 2006 increased from 19.3% to 27.5%. Furthermore, the average
value of them increased from
in 2005 to
in 2006. The ratio of isolates showing the multiple resistance between procymidone and carbendazim was 40.2%, whereas the ratio was 4.0% showing the multiple resistance in the mixture.
Variation of the Sensitivity of Botrytis cinerea causing Ginseng Grey Mold to Fungicides Inhibiting β Assembly
Kim, Joo-Hyung ; Min, Ji-Young ; Baek, Young-Soon ; Bae, Yeoung-Seuk ; Kim, Heung-Tae ;
Research in Plant Disease, volume 13, issue 3, 2007, Pages 177~182
DOI : 10.5423/RPD.2007.13.3.177
In this experiment, 236 isolates of Botrytis cinerea isolated from the lesions of ginseng grey mold in 2005 and 2006 were examined for their sensitivity to fungicide inhibiting
assembly. The baselines of fungicide resistance were determined as 10.0 and
values for carbendazim and the mixture of carbendazim and diethofencarb, respectively. The ratios of isolates resistant to carbendazim in 2005 and 2006 was investigated to be 87.6 and 96.6%, respectively. In the case of the mixture of carbendazim and diethofencarb, the ratio of the resistant isolates was 23.6% for 2005 and 24.5% for 2006. The ratio of the resistant isolates to the mixed fungicide was fluctuated according to regions where isolates of B. cinerea were obtained. In Yeoncheon of Gyeonggi Province, 4.3% of the isolates used in the experiment was resistant in 2005 and no resistant isolate was obtained in 2006. Among 5 regions, the ratio of resistant isolates was the highest as 70.0% in Yecheon of Gyeongbuk Province.
New Hosts of Ampelomyces quisqualis Hyperparasite to Powdery Mildew in Korea
Lee, Sang-Yeob ; Kim, Yong-Ki ; Kim, Hong-Gi ; Shin, Hyeon-Dong ;
Research in Plant Disease, volume 13, issue 3, 2007, Pages 183~190
DOI : 10.5423/RPD.2007.13.3.183
308 isolates of Ampelomyces quisqualis were isolated from powdery mildew fungi of 73 plant species in Korea from 1994 to 2004. Among them, the new mycohosts and new plant hosts of A. quisqualis were found in 13 species of powdery mildew fungi in 38 species of plant. The new hosts of A. quisqualis were Erysiphe heraclei on Heracleum moellenderfii; E. hommae on Elsholtzia splendins; E. glycines on Glycine max; E. lespedezae on Lespedeza biclor; E. pileae on Pilea mongolica; E. pisi on Phaseolus radiatus; E. polygoni on Rumex aquatica and Rumex crispus; Golovinomyce artemisiae on Artemisia princeps var. orientalis; G. cichoracearum on Rudbeckia laciniata var. hortensis; G. rubiae on Rubia akane; Oidium sp. on Valeriana fauriei, Lactuca indica var. laciniata, Carpesium triste var. manshuricum, Aster ageratoides var. turczaninow, Lufa cylindrica, Ixeris demtats Nakai, Phlox paniculata, Bidens bipinnata and Pathenocissus tricuspidata; Microsphaera alphitoides on Quercus aliema, M. pseudolonicerae on Cocculus trilobos; Podosphaera sp. on Ligustrum obtusifolium; Sphaerotheca aphanisi on Fragaria ananassa; S. balsaminae on Impatiens textori; S. fusca on Cucurbita pepo, Cucurbita maxima, Ligularia fischeri, Solanum melongena, Lagenaria leucantha, Cucumis melo var. makuwa, Acalypha australis, Cosmos bipinnatus and Aster scaber; Uncinuliella simulans on Rosa muliflora and Uncinula australiana on Lagerstroemia indica.
Mass Cultivation of A Hyperparasite, Ampelomyces quisqualis 94013 for Biological Control of Powdery Mildew
Lee, Sang-Yeob ; Kim, Yong-Ki ; Kim, Hong-Gi ;
Research in Plant Disease, volume 13, issue 3, 2007, Pages 191~196
DOI : 10.5423/RPD.2007.13.3.191
An isolate of Ampelomyces quisqualis 94013(AQ94013) was selected as an effective agent for biological control against cucumber powdery mildew. In order to develop mass production technique, six cereal media made with barley, rice, mille and brown rice, sorghumand rice seed were tested. Among them, barley medium was the best for the growth and conidial production of AQ94013. Optimum temperature for the mass production of AQ94013 was
and followed by
. Light radiation inhibited conidial production of AQ94013 since number of conidia formed on barely medium under continuous light or 12 hrs alternative light were much less than cultured in darkness. Tthe conidia produced on the medium at
maintained the parasitic ability to Sphaerotheca fusca for 30 days. A culture method of AQ94013 in barley liquid medium with adding barely power(40 g/l) in darkness for five days at
using a 30 l-fermenter was very effective for mass production of conidia.
Biological Control of Cucumber Powdery Mildew Using A Hyperparasite, Ampelomyces quisqualis 94013
Lee, Sang-Yeob ; Kim, Yong-Ki ; Kim, Hong-Gi ;
Research in Plant Disease, volume 13, issue 3, 2007, Pages 197~203
DOI : 10.5423/RPD.2007.13.3.197
An isolate of Ampelomyces quisqualis 94013(AQ94013) was selected as an effective parasite for biological control of cucumber powdery mildew. In the greenhouse, occurrence of cucumber powdery mildew was significantly suppressed for nine days by pre-treatment with
of conidial suspension of AQ94013. The disease was effectively controlled within three to seven days by post-treatment with the
suspension of AQ94013. When AQ94013 was treated at concentration of
three times at seven-day interval in the vinylhouse, the control effect was higher than that treated twice at ten-day interval and that treated with fenarimol twice. As the results, Ampelomyces quisqualis 94013 could be a prospective biofungicide for biological control of powdery mildew of cucumber.
Survey of Overwintering Inoculum Potential of Anthracnose of Sweet Persimmon Caused by Colletotrichum gloeosporioides
Kwon, Jin-Hyeuk ; Jeong, Seon-Gi ; Chung, Bu-Keun ;
Research in Plant Disease, volume 13, issue 3, 2007, Pages 204~206
DOI : 10.5423/RPD.2007.13.3.204
In 2006 to 2007, the potential inoculum source of the anthracnose of sweet persimmon caused by Colletotrichum gloeosporioides was surveyed. The infected twigs, buds, dead twigs, petiole, leaves, dropped fruits were collected and tested for their possibility as overwintering inoculum. The detection rates of the pathogen from various parts of sweet persimmon tree were varied. When the collected samples were examined in April. Over than 93.3% of infected twig samples were harbored mycelia of C. gloeosporioides, and 46.7% of infected buds, 36.7% of dead twigs, 23.3% of petioles, and 16.7% of leaves were beared pathogenic fungus. No pathogenic fungus were detecded from healthy twigs and buds. Infected twigs and bud was important overwintering sites and formed conidia actively in next spring. The infected twigs, leaves, petioles, and fruits in growing season produced great number of conidia and caused active dissemination of the anthracnose disease in sweet persimmon. In growing season, all of the infected parts, such as twigs, leaves, petioles, and fruits produced pathogenic fungus.
Stem Rot of English Ivy Caused by Phytophthora cinnamomi
Kim, Hyung-Moo ; Jin, Kyo-Hoon ; Ko, Jung-Ae ;
Research in Plant Disease, volume 13, issue 3, 2007, Pages 207~210
DOI : 10.5423/RPD.2007.13.3.207
A stem and root rot disease of English ivy (Hedera helix) was found in the flower market, Jeonju, Chonbuk province, Korea in May 2007. A Phytophthora species was isolated from the diseased part of the plant. Based on the mycological properties and pathogenesis the isolate was identified as Phytophthora cinnamomi. The fungus produce ovoid sporangium which was ovoid to spherical and obpyriform which is noncaducous, non-papillate and averaged
in dimension. Chlamydospores were abundantly produced on agar media and sized about
in diameter. The fungus was heterothallic and A1 mating type. Oospores were measured
in size. Optimum temperature for growth of the fungus was 25 to
. Up to our knowledge, this is the first report demonstrating the stem rot on English ivy caused by P. cinnamomi in Korea.
Occurrence of Anthracnose Caused by Glomerella cingulata on Eucaly trees in Korea
Kwon, Jin-Hyeuk ; Jeong, Seon-Gi ; Jee, Hyeong-Jin ;
Research in Plant Disease, volume 13, issue 3, 2007, Pages 211~215
DOI : 10.5423/RPD.2007.13.3.211
Since 2003, anthracnose symptoms on Eucalyptus globulus were observed in farmer's field at Jisepo, Ilwoon-myon, Geoje city, Gyeongnam province, Korea. Typical symptoms of dark brown to black spot appeared on the leaves, twigs, and stems. Infected young trees were wilted, blighted and died eventually. The pathogen isolated from the typical symptom formed gray to dark gray colony on potato dextrose agar and showed optimum growth at
. Conidia were single celled, colorless, cylindrical with obtuse ends, and
in size. Appressoria were dark brown, ovate to obovate, and
in size. Perithecia were black and globose in shape and
in size. Asci were clavate to cylindrical in shape and
in size. Ascospores were cylindrical, fusiform, slightly curved at the center, and
in size. On the basis of mycological characteristics and pathogenicity test on E. globulus, the pathogen was identified as Glomerella cingulata. This is the first report of the anthracnose on E, globulus caused by G. cingulata in Korea.
Occurrence of Gray Mold on Prunus mume Caused by Botrytis cinerea in Korea
Kwon, Jin-Hyeuk ; Kim, Won-Il ; Park, Chang-Seuk ;
Research in Plant Disease, volume 13, issue 3, 2007, Pages 216~219
DOI : 10.5423/RPD.2007.13.3.216
Gray mold caused by Botrytis cinerea occurred on Prunus mume Sieb. & Zucc. in an experimetal orchard of Gyeongsangnam-do Agricultural Research and Extension Services in Korea from 2005 to 2007. The symptoms usually started with water-soaking lesions and hollow depression on the fruit surface. The conidia of the pathogen appeared on the surface fruits. The conidia were one celled and mostly ellipsoid or ovoid in shape and were colorless or pale brown in color. The conidia were
in size and conidiophores were
in length. The sclerotial formed abundantly on potato-dextrose agar, They were flat or irregular in shape and black in color The optimum temperature for sclerotial formation was
. On the basis of mycological characteristics and pathogenicity test on host plants, the fungus was identified as Botrytis cinerea Persoon: Fries. This is the first report on gray mold of P. mume caused by B. cinerea by in Korea.
Rust of Korean Azalea (Rododendron yedoense) Caused by Chrysomyxa rhododendri
Do, Yun-Jeong ; Kim, Hyung-Moo ; Lee, Kui-Jae ; Lee, Wang-Hyu ; Ko, Jeong-Ae ;
Research in Plant Disease, volume 13, issue 3, 2007, Pages 220~222
DOI : 10.5423/RPD.2007.13.3.220
Rust disease on Korean Azalea (Rododendron yedoense) was occurred in Jeonju, Jeonbuk province of Korea. The typical symtoms of the disease appeared first as small yellowish spots on leaves. Uredinia were observed at hypophyllous of the leaves, yellow or dark yellow in color and globoid in shape with the size of
mm. Urediniospores were also observed in the leaves, yellow in color with globoid or ovoid in shape with the size of
. Surface characterization of the spores revealed that these spores have papilla-like projections, which were made of
annulation (SA; small annulate). Based on these morphological characterizations the causative fungus was identified as Chrysomyxa rhododendri.
Lichen Diseases on Prunus mume Caused by Colonization of Consortium of Several Crustose Lichens Including Parmotrema autrosinense
Koh, Young-Jin ; Kim, Gyoung-Hee ; Jeong, In-Ho ; Lim, Myoung-Taek ; Park, Hyun-Su ; Lee, Gyu-San ; Jo, Kyoung-Youn ; Hur, Jae-Seoun ;
Research in Plant Disease, volume 13, issue 3, 2007, Pages 223~227
DOI : 10.5423/RPD.2007.13.3.223
Lichen is a symbiotic association which lichen-forming fungi associate with green algae or cyanobacteria capable of producing food by photosynthesis. Lichen is one of the most widely distributed eukaryotic organisms on every kind of surface of soils, rocks and trees. Lichen-forming fungi commonly colonize on the surface of barks of dead or dying trees, which they use merely as a means of support without parasitizing. Lichen consortium of Parmotrema autrosinense and several crustose lichens heavily colonized on the surface of P. mume in Suncheon, Jeonnam province in Korea. The lichen consortium on the surface of P. mume gave the affected trees a dull gray appearance and suppressed their normal growths. Severely affected twigs or branches resulted in the death when the lichen thalli were not rubbed off the barks of P. mume. Twigs or branches of P. mume were recovered from blight syndrome when the lichen thalli were rubbed off, The recovered twigs or branches became healthy and clean when
20 or thiophanate-methyl paste were treated on the barks of P. mume. This is the first report of lichen diseases on P. mume caused by colonization of consortium of several crustose lichens including P. autrosinense.