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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Research in Plant Disease
Journal Basic Information
Journal DOI :
Korean Society of Plant Pathology
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Volume & Issues
Volume 14, Issue 3 - Dec 2008
Volume 14, Issue 2 - Aug 2008
Volume 14, Issue 1 - Apr 2008
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Survey of Viral Diseases Occurrence on Major Crops in 2007
Kim, Jeong-Soo ; Lee, Su-Heon ; Choi, Hong-Soo ; Choi, Guk-Sun ; Cho, Jeom-Deog ; Chung, Bong-Nam ;
Research in Plant Disease, volume 14, issue 1, 2008, Pages 1~9
DOI : 10.5423/RPD.2008.14.1.001
The severe damage induced by the important viruses of Rice stripe virus (RSV), Cucumber green mottle mosaic virus (CGMMV), Melon necrotic spot virus (MNSV), Tomato spotted wilt virus (TSWV) and Tomato bushy stunt virus (TBSV) was described on major crops in Korea. In 2007, the plot incidence rate of RSV was 100% on the precocious rice cultivars at the Western coastal provinces of Gyeonggido, Chungcheongnamdo, Jellabugdo and Jellanamdo, and Jejudo. RSV occurred in 2,441 ha with incidence rate of 70% over at 5 areas of Seocheon, Seosan, Boryung, Hongsung and Buyou in Chungcheongnamdo. At 4 areas of Buan, Gimje, Gunsan and Gochang in Jellabukdo, RSV occurred in 2.016 ha. CGMMV occurred on watermelon in 4.6 ha at Cheongyang area, and its outbreak was also 890 ha on oriental melon for 120 farmers with the incidence area of 23% against total cultivation areas of Seongju. MNSV was recorded firstly on watermelon in 2006 at Andong and it spread to 3 areas of Hapcheon, Gochang and Yanggu. TSWV occurred firstly at Danggin in Chungcheongnamdo in 2005. TSWV in 2006 spread to 6 areas; Taian, Hongsung and Seosan in Chungcheongnando, Namwon in Jellabukdo, and Sunchon and Kwangju in Jellanamdo. In 2007, TSWV covered 17 areas of western and southern parts; the 5 area including Taian in Chungcheongnamdo, Kwangju in Jellanamdo, Bucheon in Gyunggido, and so forth. TBSV was described firstly on table tomato at Sacheon in Kyungsangnamdo in 2004. TBSV occurred on cherry tomato at Chungju in 2006 and on table tomato at Busan area.
Occurrence and Biovar Classification of Bacterial Wilt Caused by Ralstonia solanacearum in Eggplant (Solanum melongena)
Lim, Yang-Sook ; Lee, Mun-Jung ; Cheung, Jong-Do ; Rew, Young-Hyun ; Kim, Byung-Soo ;
Research in Plant Disease, volume 14, issue 1, 2008, Pages 10~14
DOI : 10.5423/RPD.2008.14.1.010
Batcterial wilt caused by Ralstonia solanacearum is one of important and widespread diseases worldwide as well as in Korea. Bacterial wilt disease caused by R. solanacearum has been reported mainly in solanaceous crops including eggplant (Solanum melongena), tomato (Solanum lycopersicum), potato (S. tuberosum), and pepper (Capsicum annuum). A total of 48 strains of R. solanacearum from eggplant were collected during 2005 and 2006. They were confirmed as R. solanacearum by PCR amplification with primer pair flipcF/flipcR resulting in production of 470-bp DNA fragment. The 15 isolates exhibited pathogenicity on eggplant and tomato, but less virulent on pepper than other species. The biovar of collected isolates, which have been reported of five types worldwide, were classified as biovars 3 and 4 by physiological test. Biovar 4 was the dormant type without pathogenicity on eggplant rootstock, whereas biovar 3 had pathogenicity on eggplant rootstocks that is resistant to R. solanacearum, indicating necessity of breeding new rootstock with resistance to R. solanacearum biovar 3
Identification and Characterization of Three Isolates of Cucumber mosaic virus Isolated from Weed Hosts
Lee, Hyeok-Geun ; Kim, Sung-Ryul ; Jeon, Yong-Woon ; Kwon, Soon-Bae ; Ryu, Ki-Hyun ; Choi, Jang-Kyung ;
Research in Plant Disease, volume 14, issue 1, 2008, Pages 15~20
DOI : 10.5423/RPD.2008.14.1.015
Three isolates of Cucumber mosaic virus (CMV) were isolated from weed hosts showing typical mosaic symptoms, and some properties of the viruses were investigated. CMV isolates, designated as Is-CMV, Jd-CMV and Pla-CMV from Isodon inflexus, Jeffersonia dubia and Phryma leptostachya var. asiatica, respectively, were identified and characterized by biological reaction in several host plants, serological property, dsRNA analysis, reverse transcription-polymerase chain reaction (RT-PCR), restriction fragment-length polymorphism (RFLP). All isolates systemically infected in Nicotiana benthamiana, Cucurbita pepo cv. Black beauty and Cucumis sativus, and did not reveal any differences in these host plants between the isolates. However, remarkable difference in the symptoms was found between the CMVs in Capsicum annuum. Is-CMV induced an asymptomatic symptoms, while Jd-CMV and Pla-CMV produced severe mosaic symptoms in C. annuum plants. In dsRNA analysis, all isolates revealed four major bands with estimated molecular size of 3.4, 3.2, 2.1 and 1.0 kbp. The cDNAs of coat protein gene of the isolates were amplified by RT-PCR using a genus-specific single pair primers that designed to amplify a DNA fragment of approximately ranging from 938 to 966 bp. By restriction mapping analysis using RFLP of the RT-PCR products as well as by serological properties of gel diffusion test, the CMV isolates belong to a typical members of CMV subgroup IA. This is the first report on the occurrence of CMV in the three weed hosts.
Establishment of Economic Threshold by Evaluation of Yield Component and Yield Damages Caused by Rice Leaf Blast (Magnaporthe grisea)
Yeh, Wan-Hee ; Park, Hong-Hyun ; Nam, Young-Ju ; Kim, Seol-A ; Lee, Jeong-Hee ; Shim, Hong-Sik ; Kim, Yong-Ki ; Lee, Yong-Hwan ; Lee, Yeong-Hoon ;
Research in Plant Disease, volume 14, issue 1, 2008, Pages 21~25
DOI : 10.5423/RPD.2008.14.1.021
This study was conducted to decide disease incidence level of rice leaf blast required for reasonable fungicide application in paddy field. We induced the disease development by inoculating rice blast pathogens on rice seedlings (Jinmibyeo) in the greenhouse and transplanting the infected seedlings in the field two weeks after rice plants were transplanted. We scored the disease incidence, grouped and marked according to degree of percentage of diseased leaf area at maximum stage of disease development. The percent diseased leaf area (PDLA) had negative correlations with panicle number per hill, ripened grain (%), and total yield; their correlation coefficients (r),
, respectively. However, it had positive correlations with spikelets per panicle and thousand grain weight; their correlation coefficients (r),
, respectively. Gain threshold (GT) calculated based on control cost and market price was estimated to be 8.35. Economic injury level (EIL) obtained based on GT and coefficient of damage of regression equation between disease incidence and the different yield components; panicle number per hill, spikelets per panical, ripened grain(%), thousand grain weight and yield were 41.8, 9.7, 19.1, 291.1 and 3.4%, respectively. Economic threshold (ET) for yield was 2.7% (
) on PDLA. These results suggest that application of fungicide is necessary when two under leaves are almost covered with lesions or contained more than twenty large lesions under leaves at maximum tillering stage.
Fungicide Selection for Control of Lycium chinense Anthracnose Caused by Colletotrichum spp.
Koo, Han-Mo ;
Research in Plant Disease, volume 14, issue 1, 2008, Pages 26~31
DOI : 10.5423/RPD.2008.14.1.026
Anthracnose caused by Colletotrichum spp. has been known as a significant disease which commonly infects to the fruits of Chinese matrimony (Lycium chinese) in the field conditions. To select effective fungicides for the control of Chinese matrimony anthracnose, the antifungal activity of 15 fungicides were evaluated with 13 different strains of Colletotrichum spp. in the laboratory condition. Six fungicides (Dithianon WG, Tebuconazole SC, Tebuconazole WG, Trifloxystrobin + Tebuconazole SC, Azoxystrobin SC and Polyoxin D zinc salt + Carbendazim WP) out of them, showed effective suppression with the mycelium growth of pathogenic fungus, and were selected to test in vivo of the field condition. Five fungicides, Dithianon WG, Tebuconazole WG, Trifloxystrobin + Tebuconazole SC, Tebuconazole SC and Azoxystrobin SC, were significantly effective to protect anthracnose of Chinese matrimony, the variety "Chungyang Jerae".
Suppression of Bacterial Wilt in Tomato Plant Using Pseudomonas putida P84
Seo, Sang-Tae ; Park, Jong-Han ; Kim, Kyung-Hee ; Lee, Sang-Hyun ; Oh, Eun-Sung ; Shin, Sang-Chul ;
Research in Plant Disease, volume 14, issue 1, 2008, Pages 32~36
DOI : 10.5423/RPD.2008.14.1.032
Bacterial wilt caused by Ralstonia solanacearum has become a severe problem on tomato in Korea and no effective control measures are available yet. Pseudomonas species play key roles for the biocontrol of many plant diseases especially in soil. A rhizobacterial population of 150 Pseudomonas strains, isolated from the rhizosphere soil of various plants grown at different sites, was screened for 2,4-diacetylphloroglucinol producing gene (PhlD) by PCR. Two strains (P83 and P84) among them were found to be phlD positive. When the isolates were analysed by 16S rDNA (Sensu Stricto), all isolates yielded amplified products of 1,018bp. Of the 150 isolates of Pseudomonas spp., a bacterial strain P. putida P84 isolated from tomato rhizosphere showed to suppress a wide range of phytopathogenic bacteria in vitro. The best source of carbon for P84 strain were glucose, arabinose, inositol and melibiose. In greenhouse experiments, P84 strain suppressed the development of bacterial wilt in tomato with a control value of 60%.
Control of Gray Blight of Tea Plants Using a Biofungicide
Kim, Gyoung-Hee ; Lim, Myoung-Taek ; Hur, Jae-Seoun ; Yum, Kyu-Kim ; Koh, Young-Jin ;
Research in Plant Disease, volume 14, issue 1, 2008, Pages 37~42
DOI : 10.5423/RPD.2008.14.1.037
Bacillus subtilis BD0310 isolated from tea leaves was used for the development of a biofungicide against Pestitalotiopsis longiseta causing gray blight of tea plants. After mass culture of the antagonistic bacteria, the biofungicide formulated as a suspension concentrate was evaluated for its control efficacy against the gray blight of tea plant in a greenhouse and a tea plantation, respectively. Spray of the biofungicide 2 days before inoculation of P. longiseta inhibited more efficiently the development of gray blight compared with spray of the biofungicide 2 days after inoculation of the pathogen onto the leaves of tea plants in a greenhouse. In the field investigation under application of the biofungicide in 2005 and 2006, control efficiencies increased according to the number of spray of the biofungicide. Control efficiencies of the biofungicide were 52.4%, 66.7%, 71.4% and 85.7% against gray blight in 4 times spray of the biofungicide alone at 7 days interval, 6 times spray of the biofungicide alone at 7 days interval, 2 times alternate spray of biofungicide and chemical fungicide at 7 days interval and 4 times spray of chemical fungicide alone at 7 days interval, respectively. Therefore, the alternate application of the biofungicide and chemical fungicide at 7 days interval can increase the control efficiency with reduction of the amount of chemical fungicides and the number of spray for the control of gray blight of tea plants in the field.
Efficacy of Antagonistic Bacteria for Biological Control of Rhizoctonia Blight (Large patch) on Zoysiagrass
Jung, Woo-Chul ; Shin, Taek-Su ; Kim, Bong-Su ; Im, Jae-Seong ; Lee, Jae-Ho ; Kim, Jin-Won ;
Research in Plant Disease, volume 14, issue 1, 2008, Pages 43~50
DOI : 10.5423/RPD.2008.14.1.043
Rhizoctonia blight (large patch) caused by Rhizoctonia solani AG2-2 is one of the major diseases on zoysiagrass in golf courses. In this study, anatgonistic bacteria to R. solani AG2-2 were selected in vitro tests using confrontation bioassay and triple layer agar diffusion method. The most active bacteria, Bacillus subtilis CJ-9 were tested for controlling large patch in pots. Relative Performance Indies (RPI) was used as a criterion for the selection of potential biocontrol agent. B. subtilis CJ-9 showed resistance to major synthetic agrochemicals used in golf course. In field tests at golf course, B. subtilis CJ-9 was more effective in suppression of large patch severity and population development of R. solani AG2-2 in soil than chemical fungicides. B. subtilis CJ-9 could be an alternative to chemical fungicides for eco-friendly management of large patch on zoysiagrass.
Effect of COY (Cooking Oil and Yolk mixture) and ACF (Air-circulation Fan) on Control of Powdery Mildew and Production of Organic Lettuce
Jee, Hyeong-Jin ; Ryu, Kyung-Yul ; Park, Jong-Ho ; Choi, Du-Hoe ; Ryu, Gab-Hee ; Ryu, Jae-Gee ; Shen, Shun-Shan ;
Research in Plant Disease, volume 14, issue 1, 2008, Pages 51~56
DOI : 10.5423/RPD.2008.14.1.051
Powdery mildew of lettuce that is a newly reported disease became a threat to organic cultivation of lettuce in Korea since the disease caused by Podosphaera fusca resulted in a half of yield loss in heavily infected fields. To improve micro-environmental conditions around lettuce, ACF (air-circulation fan) was installed on inside roof of plastic house at 6 m intervals. The ACF increased 57% of lettuce yield and reduced 71.4% of lettuce seedling death. COY (cooking oil and yolk mixture) consisted of cooking oil 0.3% and egg yolk 0.08% reduced lettuce seedling death from 89.3% to 92.9% under the greenhouse. Seven-day interval spray of COY resulted in high control values of powdery mildew of lettuce ranging from 89.6% to 96.3%, which was comparable to a fungicide, Azoxystrobin. Lettuce yield was increased about two times compared to a non-treated conventional cultivation. Qualities of lettuce such as hardness and chlorophyll content were also improved by COY and ACF combination. Effect of COY on control of the disease was improved when
1,000 ppm was supplemented. Results indicated that the COY made of cooking oil such as canola emulsified with yolk was highly effective on control of powdery mildew of lettuce and suitable for organic agriculture, especially when combined with ACF.
Occurrence of Rhizopus Soft Rot on Apple Fruit Caused by Rhizopus stolonifer in Korea
Kwon, Jin-Hyeuk ; Jee, Hyeong-Jin ;
Research in Plant Disease, volume 14, issue 1, 2008, Pages 57~60
DOI : 10.5423/RPD.2008.14.1.057
A rhizopus soft rot caused by Rhizopus stolonifer occurred sporadically on apple fruits (Malus pumila var. dulcissima Koidz.) at a wholesale market of agricultural products in Jinju, Korea in 2006 and 2007. Infected fruits were rapidly water-soaked, softened and rotted. The symptoms were initiated mainly from wounds or cracks occurred at harvest time. Sporangiophores were
in length and
in width. Sporangia were globose or hemispheric and
in size. The color of sporangia was white and cottony at first and gradually turned to brownish black, bearing abundant spores when matured. Columella were hemispheric and
in size. Sporangiospores were irregular round or oval, brownish-black streaked and
in size. Optimum temperature for mycelial growth of the fungus on PDA was
. On the basis of mycological characteristics and pathogenicity to apple the causal fungus was identified as Rhizopus stolonifer (Ehrenb.) Vuill. This is the first report of Rhizopus soft rot by R. stolonifer on Fuji apple in Korea.
Anthracnose of Blueberry (Vaccinium spp.) of Caused by Colletotrichum gloeosporioides in Korea
Kwon, Jin-Hyeuk ; Cheon, Mi-Geon ; Park, Chang-Seuk ;
Research in Plant Disease, volume 14, issue 1, 2008, Pages 61~63
DOI : 10.5423/RPD.2008.14.1.061
Anthracnose of blueberry (Vaccinium spp.) caused by Colletotrichum gloeosporioides was observed in the exhibition orchard of Gyeongsangnam-do Agricultural Research and Extension Services in Korea, 2007. The typical symptoms were initially water-soaked lesions and then rotten on fruits, the infected fruits were dropped but some of them were hunged and mummified. The pathogenic fungus grown on potato dextrose agar showed greyish to dark gray color. Conidia were straight, cylindrical apex obtuse in shape and
in size. Appressoria were brown in color, clavate or ovate in shape and
in size. Optimum temperature for mycelial growth was
. On the basis of mycological characteristics and pathogenicity test on host plants, the fungus was identified as Colletotrichum gloeosporioides (Penz.) Penz. & Sacc.. This is the first report on the anthracnose of blueberry (Vaccinium spp.) caused by C. gloeosporioides in Korea.
Petiole and Root Rot on Spathiphyllum Caused by Cylindrocladium spathiphylli in Korea
Han, Kyung-Sook ; Park, Jong-Han ; Han, You-Kyoung ; Cheong, Seung-Ryong ;
Research in Plant Disease, volume 14, issue 1, 2008, Pages 64~67
DOI : 10.5423/RPD.2008.14.1.064
In 2005 and 2007, petiole and root rot of Spathiphyllum spp. was occurred at commercial greenhouse during summer (June-September) in Koyang city and Yongin city, Kyunggi-do, Korea. The pathogenic fungus was isolated from diseased plants and the cultural and morphological chracteristic were observed. Conidia were rod in shape, 1-3 septa and
) in size. The optimum temperature for the mycelial growth of the isolates was
. According to result the pathogenicity test, first disease symptoms appeared five days after inoculation. On the basis of mycological characteristics and pathogenicity test on host plants, the fungus was identified as Cylindrocladium spathiphylli. This is the first report on Spathiphyllum spp. caused by C spathiphylli in Korea.
Brown Ring Spot on Leaves of Kiwifruit Caused by Alternaria alternata
Jeong, In-Ho ; Kim, Gyung-Hee ; Lim, Myoung-Taek ; Hur, Jae-Seoun ; Shin, Jong-Sup ; Koh, Young-Jin ;
Research in Plant Disease, volume 14, issue 1, 2008, Pages 68~70
DOI : 10.5423/RPD.2008.14.1.068
Brown leaf spots on leaves of kiwifruit(Actinidia deliciosa) were observed at farmers` orchards in Suncheon and Goheung, Jeonnam Province, Korea in June, 2006. They developed to form dark brown ring spots and severely infected leaves resulted in defoliation during the growing season of kiwifruit. Alternaria sp. was isolated from the diseased leaves repeatedly and was identified as Alternaria alternata on the basis of its mycological characteristics on potato dextrose agar and its pathogenicity was confirmed by wound inoculation on healthy leaves of kiwifruit. A. alternata formed gray to dark sooty gray colony and produced numerous conidia on potato dextrose agar. The conidia, commonly in long chains of 5 or more produced on conidiophores, have
longitudinal septa and mostly ovoid or obclavate in shape and were pale brown golden brown in color. The condia were
in size and conidiophores were
in length. This is the first report on the brown ring spot on leaves of kiwifruit caused by A. alternata in Korea.
Some Properties of an Isolate of Peanut stunt virus Isolated from White Clover (Trifolium repens L.)
Jung, Goo-Ho ; Jeon, Yong-Woon ; Choi, Jang-Kyung ; Hong, Jin-Sung ; Ryu, Ki-Hyun ; Lee, Sang-Yong ;
Research in Plant Disease, volume 14, issue 1, 2008, Pages 71~75
DOI : 10.5423/RPD.2008.14.1.071
An isolate of Peanut stunt virus (PSV), named as Tr-PSV, was isolated from white clover (Trifolium repens L) showing mosaic symptom. Tr-PSV systemically infected all plants tested in the Nicotiana spp. and induced local lesions on inoculated leaves of Chenopodium amaranticolor. However, Tr-PSV induced typical mosaic symptoms as ER-PSV on Vigna unguiculata 5 to 6 days after inoculation, while Fny-CMV used as a control virus of Cucumovirus produced local lesions on inoculated leaves. In dsRNA analysis, Tr-PSV consisted of four dsRNAs, but satellite RNA was not detected. The cDNA of coat protein gene of Tr-PSV was amplified by RT-PCR using a Cucumovirus-specific single pair primers that designed to amplify a DNA fragment of approximately 950 bp. By restriction mapping analysis using RFLP of the RT-PCR products and by serological properties of gel diffusion test, Tr-PSV belongs to a typical member of PSV subgroup I. This is the first report on the occurrence of PSV in white clover in Korea.
Allium tuberosum, a New Host of Root-knot Nematode, Meloidogyne Incognita in Korea
Kim, Dong-Geun ; Lee, Joong-Hwan ;
Research in Plant Disease, volume 14, issue 1, 2008, Pages 76~78
DOI : 10.5423/RPD.2008.14.1.076
During the investigation of Allium decline in Pohang, Korea, root-knot nematode was found from root of Allium tuberosum Roth. It was identified as Meloidogyne incognita and was first reported from Allium tuberosum. Allium decline was associated with root-knot nematode, root mite and Fusarium sp. but root-knot nematode appeared to be the main cause of Allium decline.