Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Research in Plant Disease
Journal Basic Information
Journal DOI :
Korean Society of Plant Pathology
Editor in Chief :
Volume & Issues
Volume 15, Issue 3 - Dec 2009
Volume 15, Issue 2 - Aug 2009
Volume 15, Issue 1 - Apr 2009
Selecting the target year
Convenient Genetic Diagnosis of Virion Captured (VC)/RT-PCR for Rice Viruses (RSV, RBSDV) and Small Brown Plant Hopper
Kim, Jeong-Soo ; Lee, Su-Heon ; Choi, Hong-Soo ; Cho, Jeom-Deog ; Noh, Tai-Whan ; Kim, Jin-Young ;
Research in Plant Disease, volume 15, issue 2, 2009, Pages 57~62
DOI : 10.5423/RPD.2009.15.2.057
Genetic diagnosis method of Virion Captured (VC)/RT-PCR for Rice stripe virus (RSV) and Rice black-streaked dwarf virus (RBSDV), Korean major rice viruses transmitted by small brown plant hopper, Laodelphax striatellus, was developed. Virion extraction buffer for rice plant was 0.01M potassium phosphate buffer, pH 7.0, containing 0.5% sodium sulfite. However, the extraction buffer for L. striatellus was 0.01M potassium phosphate buffer, pH 7.0, containing 0.5% sodium sulfite and 2% polyvinylpyrrolidone wt 40,000 (PVP-40). Specific primers for detection of RSV and RBSDV were selected for VC/RT-PCR method. The specific primers were used as a duplex primer to detect viruliferous small brown plant hopper collected from Gimpo, Pyeongtaek and Siheung areas in Gyeonggi province. The genetic diagnosis methods of single and duplex VC/RT-PCR for RSV and RBSDV could be used easily and economically, especially on the diagnosis of L. striatellus. The rate of viruliferous insect (RVI) for RSV was compared with ELISA and VC/RT-PCR for L. striatellus collected from fields. RVI by ELISA was same as 9.2% with RVI by VC/RT-PCR. However, there were some different detection results between the methods. It could be suggested that there is a possibility of serological and/or genomic differences among RSV isolates. The portion of RVI detected simultaneously by ELISA and VC/RT-PCR was 71.0%, and the detection rate from VC/RT-PCR was higher as 3.2% than that from ELISA, which had a reason of simultaneous detection ability both RSV and RBSDV of VC/RT-PCR.
New Alternate Host of Rice stripe virus - `Deulmuksae`
Yoon, Young-Nam ; Lee, Bong-Choon ; Jung, Ji-Hun ; Kim, Jung-In ; Hwang, Jae-Bok ; Kim, Chang-Seok ; Hong, Sung-Jun ; Kang, Hang-Won ; Song, Suk-Bo ; Hong, Yeun-Gyu ; Park, Sung-Tae ; Lee, Key-Woon ;
Research in Plant Disease, volume 15, issue 2, 2009, Pages 63~67
DOI : 10.5423/RPD.2009.15.2.063
Rice stripe virus (RSV) is one of Tenuivirus Group, which is carried by small brown planthopper. There is an outbreak of RSV in South Korea at 20Ot, and 2007. The infection caused by RSV had been investigated on weeds around the rice cultivated areas 13 region and 26 site including Jeonbuk Buan and Chungnam Seocheon. There have a doubt as to alternate host of RSV is total 15 Family and 50 Species including Gramineae 24 species of Duksaepul (Alopecurus aequalis), H. sativum var. vulgare etc.. There is identified the infected RSV in Festuca myuros, Alopecurus aequalis, Hordeum sativum var. vulgare, Trisetum bifidum, Echinochloa crus-galli var. crus-galli, Digitaria ciliaris among this species. Deulmuksae is the overwintering exotic weed which sprout in Autumn and wither in Spring and commonly growed as green manure crop or cover crop. In order to identify the infection rate furthermore, 111 samples which were collected at Buan Gyehwa-myeon region, and 50 samples from Seocheon Maseo-myeon in June, 2008, were ELISA tested. The results are 32 positives from Buan, 28.8% infection rate, 8 positives from Seocheon 16.0% infection rate. RSV infection of Deulmuksae is not reported currently, and follow report first describes the Deulmuksae as an alternate host of RSV.
Cucumber green mottle mosaic virus Moved into the Non-Host Figleaf Gourd Passing through Cucumber in Grafting System
Choi, Gug-Seoun ; Lee, Jin-A ; Cho, Jeom-Deog ; Chung, Bong-Nam ; Cho, In-Sook ;
Research in Plant Disease, volume 15, issue 2, 2009, Pages 68~71
DOI : 10.5423/RPD.2009.15.2.068
Cucumber green mottle mosaic virus (CGMMV) was not infected in figleaf gourd by sap inoculation. However CGMMV was detected by RT-PCR from the figleaf gourd collected from a field growing cucumber grafted onto figleaf gourd in Cheonan, Chungcheongnam Province in 2008. Which field showed 100% infection rate of the virus disease. In the experiment grafted with cucumber onto figleaf gourd, transportation of CGMMV through cucumber to figleaf gourd was confirmed by RT-PCR when the virus was mechanically inoculated on the leaves of the cucumber. The amplified DNA concentration of the virus on electrophoresis gel was much higher in the cucumber than in the figleaf gourd. However, the virus particles from the figleaf gourds were not observed under electron microscopy, also sap of the figleaf gourds was not transmittable to Nicotiana benthamiana. To identify the existence of CGMMV particle, the virus was purified from figleaf gourd and cucumber growing together in the graft system. CGMMV solution extracted from the cucumber represented a typical absorption spectrum of the virus but that from the figleaf gourd did not. Only a few CGMMV particles were observed in the purified preparation from the figleaf gourd. These results confirmed that CGMMV only passed through figleaf gourd in the grafting system. This study indicated that figleaf gourd is not a host of CGMMY.
Responses of Resistant Genes to Barley Yellow Mosaic Virus (BaYMV) Strains in Korea
Park, Jong-Chul ; Noh, Tae-Hwan ; Park, Chul-Soo ; Kang, Chun-Sik ; Kang, Mi-Hyung ; Lee, Eun-Sook ; Lee, Jun-Hee ; Lee, Jung-Joon ; Kim, Tae-Soo ;
Research in Plant Disease, volume 15, issue 2, 2009, Pages 72~76
DOI : 10.5423/RPD.2009.15.2.072
It was investigated the responses of BaYMV resistant genes to Korean BaYMV(Barley yellow mosaic virus) strains. BaYMV was distributed dominantly with about 51% detection ratio among the three investigated virus such as BaYMV, BaMMV(Barley mild mosaic virus) and SBWMV(Soil-borne wheat mosaic virus) in ELISA test. Double infection with BaYMV and BaMMV was detected also higher as 38.8%, however, BaMMV sole infection ratio was lower with only 1.4%. The 11 BaYMV resistant genes were tested their responses to four Korean BaYMV strains, BaYMV-N, H, I and M. Generally, rym 3 genes showed resistant to Korean BaYMV strains and rym 4m and 5a also was better. Three genes, rym 1+5(Mokusekko-3), rym 3(Ea 52, Baitori) and rym 5a(Solan) showed resistant responses to BaYMV-N type. In -H strain test, seven genes that rym 2(Mihori Hadaka 3), rym 3(Ea 52, Haganemugi, Baitori), rym 4m(Diana, Franka), rym 5a(Solan), rym 7(Hor 3365), rym 9(Bulgarian 347), rym 12(Jochiwon Covered 2) were considered as resistant. The three genes that rym 1+5, rym 3 and rym 5a was effective to -I strain, and rym 3, rym 4m and rym 5a showed resistant to -M strain.
Characterization of Agrobacterium spp. Isolated from Roots of the Crown Gall-infected Grapevine in Chungbuk
Yang, Seung-Up ; Park, Se-Jung ; Lee, Young-Kee ; Cha, Jae-Soon ;
Research in Plant Disease, volume 15, issue 2, 2009, Pages 77~82
DOI : 10.5423/RPD.2009.15.2.077
The roots of grapevine in the field in which the crown gall was occurred severely in Chungbuk province were collected and Agrobacterium spp. were isolated from the roots using the selective media. The selected 13 isolates were identified as A. tumefaciens with fatty acid analysis using MIDI system, nucleotide sequence of 16S rDNA, biochemical characteristics, and PCR with the species-specific primers. A. vitis, a pathogen of crown gall disease of grapevine was not isolated from the roots. All of the isolates did not show pathogenicity on the tomato seedlings and the stem and root of grapevine. Eric-PCR showed that DNA band patterns of the root isolates were a little more similar to A. tumefaciens than A. vitis. However, overall similarity between the root isolates and the pathogenic strains of A. tumefaciens and A. vitis was low by rep-PCR. These results suggest that a pathogen causing crown gall in grapevine in Chungbuk province may transmitted through the seedlings rather than via soil or roots.
Direct PCR Detection of the Causal Agents, Soybean Bacterial Pustule, Xanthomonas axonopodis pv. glycines in Soybean Seeds
Lee, Yong-Ju ; Kang, Mi-Hyung ; Noh, Tae-Hwan ; Lee, Du-Ku ; Lee, Geon-Hwi ; Kim, Si-Ju ;
Research in Plant Disease, volume 15, issue 2, 2009, Pages 83~87
DOI : 10.5423/RPD.2009.15.2.083
Direct Polymerase Chain Reaction (PCR) method that combines biological and enzymatic amplification of PCR targets was developed for the detection of Xanthomonas axonopodis pv. glycines on soybeen seeds without DNA isolation. Primers Xag F1 and Xag R1 were designed to specifically amplify a 401 bp fragment of the glycinecin A gene of X axonopodis pv. glycines. Xag F1 and Xag R1 were used to carry out the PCR analysis with genomic DNA from 45 different bacterial strains including phylogenetically related bacteria with X axonopodis pv. glycines, and other bacterial strains of different genus and species. The PCR assay using this set of primers were able to detect X axonopodis pv. glycines with DNA concentration as low as 200 fg and
cfu/ml. The Xag was detected from the seed samples incubated for 2 hrs with shaking and the intensity of the band was increase with the incubation time of seeds. The Direct PCR assay method without DNA isolation makes detection of X. axonopodis pv. glycines on soybean seeds easier and more sensitive than other conventional methods. The developed seed assay using direct PCR method will be useful for the specific detection of X. axonopodis pv. glycines in soybean seed samples.
Pathogenicity and Occurrence of Pepper Seedling Anthracnose Caused by Colletotrichum acutatum
Han, Kyung-Sook ; Park, Jong-Han ; Han, You-Kyoung ; Hwang, Jung-Hwan ;
Research in Plant Disease, volume 15, issue 2, 2009, Pages 88~93
DOI : 10.5423/RPD.2009.15.2.088
In 2008, leaf rot and blight on pepper seeding ("Dokya-chungchung") occurred in a pepper farm at Hwaseong-si, Gyeonggi-do, Korea. The typical symptom is water-soaking and dark brown leaf blight at edges and tips of leaves. The fungal colonies isolated from infected tissues were pinkish at first and turned gradually to gray. Conidia were fusiform, non-septum, and
in size. Several specific PCR primers derived from the sequence of the internal transcribed spacer (ITS) region of the rDNA, such as CaINT, CgINT and CcINT were used for the identification of the fungal pathogen. The C. acutatum-specific primer CaINT was amplified single fragment of 496 bp that discriminated C. acutatum from the other species. The pathogenicity test was performed on seedlings and fruits of red pepper. On the basis of the morphological, molecular characteristics and pathogenicity test, we identified as Colletotrichum acutatum. This is the first report on leaf rot and blight on pepper seedling caused by C. acutatum in Korea.
Control of Bakanae Disease of Rice by Seed Soaking into the Mixed Solution of Procholraz and Fludioxnil
Park, Woo-Sik ; Choi, Hyo-Won ; Han, Seong-Suk ; Shin, Dong-Beum ; Shim, Hyeong-Kwon ; Jung, En-Seon ; Lee, Se-Weon ; Lim, Chun-Keun ; Lee, Yong-Hwan ;
Research in Plant Disease, volume 15, issue 2, 2009, Pages 94~100
DOI : 10.5423/RPD.2009.15.2.094
These experiments were conducted to improve the effect of seed disinfection on rice seed severely infected Bakanae disease by seed soaking into mixed solution of prochloraz EC and fludioxonil FS. We investigated the effects of various concentrations of two fungicides mixed solution on spore germination and mycelial growth of Fusarium fujikuroi. Mycelial growth was inhibited 100% at
/ml of prochloraz and 33.3% at
/ml of fludioxonil. Spore germination was inhibited 81.4% at
/ml of prochloraz. Interestingly, mixed solution of
/ml of each fungicide inhibitied 100% of mycelial growoth and 99.2% of spore germination, respectively. Severely infected rice seeds soaked into mixed solution composed of
/ml of prochloraz and
/ml of fludioxonil showed 2.1% of disease symptoms compared to 20.4% of prochloraz
/ml, but higher concentrations of prochloraz decreased the seedling stand rate. When the seed soaking time was longer and temperature was higher, control effect on Bakanae disease was improved, but seedling stand was lower about 80% over
The Effect of the Colonization of Serratia plymuthica A21-4 in Rhizosphere Soil and Root of Pepper in Different Soil Environment
Cao, Pu ; Shen, Shun-Shan ; Wen, Cai-Yi ; Song, Shuang ; Park, Chang-Seuk ;
Research in Plant Disease, volume 15, issue 2, 2009, Pages 101~105
DOI : 10.5423/RPD.2009.15.2.101
The biocontrol agent Serratia plymuthica A21-4 was selected and proved as an excellent inhibitor of Phytophthora blight of pepper through in vitro and in vivo experiments in previous studies. To enhance the colonizing density of S. plymuthica A21-4 on plant root and rhizosphere soil, some soil conditions might effect on the colonization of the bacteria were examined. The results obtained from the study indicated that the soils containing more sand were favorable to root colonization of S. plymuthica A21-4. Organic amendment such as 3% maize straw(w/w) was helpful to colonize the bacteria in root and soil. The soil temperature about
, water content around 40%, and soil pH near to neutral or slightly acidic, were optimum condition for the colonization of S. plymuthica A21-4 in the rhizosphere soil and roots of pepper. In addition, existence of indigenous biotic entities was beneficial to the colonization of S. plymuthica A21-4.
Soil-blending Effect of Eggshell Powder on the Control of Club root Disease and the Growth of Chinese Cabbage in the Field
Gao, Yuliang ; Kim, Byeong-Kwan ; Lim, Tae-Heon ; Li, Kui-Hua ; Paek, Kee-Yoeup ; Cha, Byeong-Jin ;
Research in Plant Disease, volume 15, issue 2, 2009, Pages 106~111
DOI : 10.5423/RPD.2009.15.2.106
Before transplanting Chinese cabbage seedlings, two kinds of eggshell powder were blended into the soil of cabbage field where the club root pathogen, Plasmodiophora brassicae, was infested. The incidence of clubroot disease, the shoot and root growth of cabbages, and soil pH were examined four times at 10 to 13 days interval from transplanting Chinese cabbage. As results, the cabbages treated with eggshell powder without membrane showed the fastest growth in above ground part, and the lowest disease index for clubroot disease. The cabbages treated with eggshell powder with membrane showed better growth than the cabbages of non-treated check, but lower growth than those treated with eggshell powder without membrane. Soil pH started to increase from 3 weeks after soil blending of eggshell powder, and it reached to above 8.0. However, the soil pH of non-treated check stayed at around 6.8. In the experiment to compare the effect of eggshell powder with other calcium compounds, soil-blending of
resulted the lowest disease incidence of 1.7 and the registered fungicide, `flusulfamide`, and the resistant variety `CR Green cabbage` followed with the incidence of 1.9. Cabbages of non-treated check scored the highest disease incidence, 3.4, and that of eggshell powder without membrane was as high as 2.7. However, the growth of Chinese cabbage showed the different pattern to the disease incidence. Chinese cabbages treated with eggshell without membrane recorded the highest average growth, around 2.1 kg. On the other hand, the average growth of CR Green Chinese cabbage was about 2.0 kg, that of flusulfamide-treatment plot was 1.7, and that of non-treated check was as low as 1.3 kg. Soil blending of eggshell powder without membrane did not inhibit the development of clubroot, but increased the growth of cabbage to a great extent. Therefore, it was confirmed that soil blending of eggshell powder before transplanting makes the Chinese cabbage culture possible even in the field infested with club root pathogen.
Identification and Biological Activity of Two New Phytotoxins Isolated from Botrytis cinerea
Kim, Geum-Jung ; Yoon, Mi-Young ; Kim, Heung-Tae ; Choi, Gyung-Ja ; Jang, Kyoung-Soo ; Choi, Yong-Ho ; Park, Myung-Soo ; Cha, Byeong-Jin ; Kim, Jin-Cheol ;
Research in Plant Disease, volume 15, issue 2, 2009, Pages 112~119
DOI : 10.5423/RPD.2009.15.2.112
We discovered two novel phytotoxins produced by the pathogenic fungus, Botrytis cinerea. Among the twenty-five B. cinerea isolates, which were obtained from various host plants in 1994 and 1996, twenty-two showed strong or moderate pathogenicity on five plants such as cucumber, tomato, red pepper, tobacco and Chinese cabbage. The culture filtrate of the B. cinerea 2-16 strain showed the most potent phytotoxic activity in a tobacco leaf-wounding assay. Two novel phytotoxins were isolated from the liquid cultures of B. cinerea 2-16 by ethyl acetate extraction, flash silica gel column chromatography, silica gel column chromatography, Sephadex LH-20 column chromatography, preparative TLC and subsequently preparative HPLC. Their chemical structures were determined to be 3-O-acetyl botcinol and 3-O-acetyl botcinolide, respectively, by mass and NMR spectral analyses. These two phytotoxins caused leaf necrosis in a leaf-wounding bioassay, and significant electrolyte leakage from leaf tissues of tobacco. In the two bioassays tested, 3-O-acetyl botcinol exhibited stronger phytotoxic activity than 3-O-acetyl botcinolide. This is the first report on the production of both 3-O-acetyl botcinol and 3-O-acetyl botcinolide from B. cinerea.
Role of Two New Phytotoxins in the Pathogenicity of Botrytis cinerea
Kim, Geum-Jung ; Yoon, Mi-Young ; Kim, Heung-Tae ; Choi, Gyung-Ja ; Jang, Kyoung-Soo ; Choi, Yong-Ho ; Park, Myung-Soo ; Cha, Byeong-Jin ; Kim, Jin-Cheol ;
Research in Plant Disease, volume 15, issue 2, 2009, Pages 120~126
DOI : 10.5423/RPD.2009.15.2.120
In the course of study on the roles of phytotoxins in the pathogenicity of Botrytis cinerea, we isolated two novel phytotoxins. They were identified as 3-O-acetyl botcinol and 3-O-acetyl botcinolide. In this study, we investigated correlation between the two phytotoxins and the pathogenicity of B. cinerea. In liquid cultures, the two phytotoxins were not produced by three low pathogenic isolates out of 25 B. cinerea isolates. Among strong or moderate pathogenic isolates, some produced the two phytotoxins, but the others did not. On the other hand, the ethyl acetate extracts of fermentation broths of 10 out of 25 isolates showed phytotoxic activity against various plants tested in a whole plant assay. The phytotoxins were detected in all of the 10 phytotoxic ethyl acetate extracts. In planta, the two phytotoxins were detected in all of the plant tissues infected with strong pathogenic isolates. However, there was no correlation between the ability of B. cinerea isolates to produce the two phytotoxins and their pathogenicities. The two phytotoxins began to detect in tomato plant tissues infected with B. cinerea 2-16 at 3 days after inoculation, increased gradually till 4 days after inoculation, and then decreased. The above results suggest that 3-O-acetyl botcinol and 3-O-acetyl botcinolide are one of pathogenicity factors for B. cinerea, but not a primary determinant of its pathogenicity.
Occurrence of Gray Mold on Astragalus sinicus L. Caused by Botrytis cinerea in Korea
Kwon, Jin-Hyeuk ; Lee, Heung-Su ; Oh, In-Seok ;
Research in Plant Disease, volume 15, issue 2, 2009, Pages 127~129
DOI : 10.5423/RPD.2009.15.2.127
Gray mold caused by Botrytis cinerea occurred on Astragalus sinicus in an experimetal rice field of Gyeongsangnam-do Agricultural Research and Extension Services in Korea from 2008 to 2009. The symptoms appeared on the leaves, petiole and stems of infected plants. and then started with water-soaked, rotted and eventually died blight on plants. The colony were grayish brown and the optimum temperature for sclerotial formation was
on potato dextrose agar. The conidia were one celled, mostly ellipsoid or ovoid in shape, and were colorless or pale brown in color. The conidia were
in size and conidiophores were
in length. On the basis of mycological characteristics and pathogenicity test on host plants, the fungus was identified as Botrytis cinerea Persoon: Fries.
Bactericidal Effects of Nano-silver Liquid Against Various Plant Pathogenic Bacteria
Kim, Sang-Woo ; Min, Ji-Seon ; Lee, Youn-Su ;
Research in Plant Disease, volume 15, issue 2, 2009, Pages 130~133
DOI : 10.5423/RPD.2009.15.2.130
We have conducted in vitro experiments with nano-silver liquid for their effect against various plant pathogenic bacteria. Different types of nano-silver liquid WA-CV-WA13B, WA-AT-WB13R and WA-PR-WB13R were used. These are classified based on different manufacturing processes. The tested bacteria were provided by KACC. We experimented ten bacterial isolates in Clavibacter, Erwinia, Pseudomonas, Ralstonia, and Xanthomonas genera. In order to determine the level of concentrations of control effects, different concentrations (10, 25, 50, and 100 ppm) of each different nano-silver liquid were added in the culture media. As a result, WA-CV-WA13B showed high inhibition effect against C-1 at 10 ppm, and showed minor inhibition effects against P-6, X-1, and X-2. WA-AT-WB13R showed bactericidal effect against P-6 at 10 ppm. At 10 ppm, WA-AT-WB13R showed relatively high inhibition effects against C-1, X-1, and X-2. WA-PR-WB13R showed bactericidal effects against P-5, P-6 and X-2 at 10 ppm or higher concentrations. All the tested three nano-silver liquid showed bactericidal effects against all the tested plant pathogenic bacteria at concentrations of 25 ppm or higher. These results indicated the possible use of nano-silver liquid for the control of plant pathogenic bacteria.