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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Research in Plant Disease
Journal Basic Information
Journal DOI :
Korean Society of Plant Pathology
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Volume & Issues
Volume 15, Issue 3 - Dec 2009
Volume 15, Issue 2 - Aug 2009
Volume 15, Issue 1 - Apr 2009
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Comparative Analysis of Coat Protein Gene of Isolates of Cucumber mosaic virus Isolated from Pepper Plants in Two GMO Environmental Risk Assessment Fields
Hong, Jin-Sung ; Park, Ho-Seop ; Ryu, Ki-Hyun ; Choi, Jang-Kyung ;
Research in Plant Disease, volume 15, issue 3, 2009, Pages 165~169
DOI : 10.5423/RPD.2009.15.3.165
Twelve Cucumber mosaic virus (CMV) isolates were isolated from genetically modified (GM) and non-GM Capsicum annuum in two GM fields, Namyangju and Anseong, and their properties were investigated in this study. Coat protein (CP) gene of the CMV isolates were synthesized by RT-PCR using genus-specific primers which designed to amplify a DNA fragment of 950 bp. Purified cDNA fragments were cloned into the pGEMT easy vector for sequence determination. Nucleotide sequences (internal 657 bp) of CMV isolates were compared with Fny-CMV CP sequences and there were no significant collection site specific sequence similarities found. When predicted amino acid sequences (219 amino acids) were compared with Fny-CMV CP amino acids sequences, there were 96.8% to 97.3% similarities found from Namyangju collections and 95.9% to 96.8% similarities from Anseong collections. The phylogenetic analysis with nucleotide sequences showed definite differences in CMVs which have been isolated from the two regions.
Characterization of Prunus necrotic ringspot virus Isolate from Peach in Korea
Kim, Hyun-Ran ; Lee, Sin-Ho ; Shin, Il-Sheob ; Kim, Jeong-Hee ; Cho, Kang-Hee ; Heo, Seong ; Kim, Jeong-Soo ; Choi, Yong-Mun ;
Research in Plant Disease, volume 15, issue 3, 2009, Pages 170~174
DOI : 10.5423/RPD.2009.15.3.170
In this paper, we report a characterization of Prunus necrotic ringspot virus (PNRSV) isolate. The virus was identified from 'Yumyeong' peach showing mild mosaic on leaves in commercial orchard of 'Umsung', Chungbuk province in Korea. The virus isolate produced ringspot symptom on the inoculated cotyledons and systemic mosaic and malformation on the upper leaves of Cucumis sativus. Systemic mottles were appeared in Chenopodium quinoa. When the buds of the virus infected stem were grafted on the healthy young Prunus persica GF305 seedlings, line pattern with mosaic appeared within 3 months. Isometric virus-like particles were found in parenchyma cells and plasmodesmata of C. sativus leaves inoculated mechanically with the virus. The cDNA fragments of PNRSV coat protein (CP) region, approximately 675bp, were synthesized from genomic RNA extracted from virus-infected leaves by RT-PCR using specific primer pairs. Partial nucleotide sequences of the CP regions were determined and analyzed with the known PNRSV. The CP gene of PNRSVKorea isolates showed 93.9~94.7% similarity to the 4 known PNRSV isolates.
Effect of the Infection Times by Zucchini yellow mosaic virus on the Yield and Growth in Cucumber
Ko, Sug-Ju ; Kim, Do-Ik ; Kang, Beom-Ryong ; Kim, Seon-Gon ; Lee, Yong-Hwan ; Choi, Hong-Soo ;
Research in Plant Disease, volume 15, issue 3, 2009, Pages 175~182
DOI : 10.5423/RPD.2009.15.3.175
We investigated the effect of the infection times and infection degrees at transplanting time of Zucchini yellow mosaic virus (ZYMV) on the growth and yield of cucumber plants at the semi-forcing and the retarding culture in 2007. When cucumber was inoculated with ZYMV at transplanting time, 15, 30, 45, 60, and 75 days after transplanting, vine length, internode length, number of leaf of the plants and marketable yield largely decreased as the cucumber infected earlier. The regression models were obtained between the disease incidence levels at 20 days after transplanting as the inoculation degrees and the marketable yield of cucumber: y =
= 0.8946) at the semi-forcing culture and y =
= 0.8735) at the retarding culture. And the cucumber yield loss regression models between the symptoms incidence days from the final harvesting time and the rates of yield decrease were expressed as y=1.0851x - 6.7067 (
= 0.9567) at the semi-forcing culture andy=1.0439x + 2.1321 (
= 0.9674) at the retarding culture.
Incidence of Bacterial Brown Spot of Phalenopsis Orchids Caused by Acidovorax avenae subsp. cattleyae
Han, Kyung-Sook ; Lee, Seung-Don ; Park, Jong-Han ; Han, You-Kyoung ; Kim, Dae-Hyun ; Lee, Jung-Sup ;
Research in Plant Disease, volume 15, issue 3, 2009, Pages 183~186
DOI : 10.5423/RPD.2009.15.3.183
Leaf spot symptoms were observed in a commercial Palenopsis cultivated fields. Circular-elliptical watersoaked spots surrounded by a light green or yellow halo and turned to black when it was severe infection or blacken with gathered symptoms. These spots were finally enlarged to form of larger areas. Under the favorable conditions of temperature and moisture, the infection extended rapidly and dispersed to the crown and sometimes the infected plant became dead. A bacterial organism, isolated from the advancing margins of the lesions, was tested to characterize causing bacterium based on pathogenicity. The biochemical and physiological tests of that bacterium identified that as an Acidovorax avenae subsp. cattleyae. Therefore, we suggested to call that the new Phalaenopsis disease was bacterial brown spot caused by A.avenae subsp. cattleyae in Korea.
Effect of Temperature, Relative Humidity on Germination and Development of Powdery Mildew (Leveillula taurica) on Pepper and Its Inoculation Method
Kim, Dae-Hyun ; Park, Jong-Han ; Lee, Jung-Sup ; Han, Kyung-Sook ; Han, You-Kyoung ; Hwang, Jeong-Hwan ;
Research in Plant Disease, volume 15, issue 3, 2009, Pages 187~192
DOI : 10.5423/RPD.2009.15.3.187
Pepper powdery mildew, Leveillula taurica is one of the most important pathogens of pepper in the greenhouses and fields in Korea and is becoming a worldwide disease. These experiments were carried out to investigate the optimal germination, disease development conditions, disease incidence and effective inoculation methods of pepper powdery mildew. The incidence of powdery mildew was investigated throughout the country based on the major pepper growing areas in 2009. The average rate of infected leaves ranged from 15.3% to 81.8% in greenhouses and fields. Powdery mildew incidences were more severe in greenhouses than those of fields. The optimal temperature for conidial germination was
and showed the highest germination at 6 hs after treatment. The range of temperature for germination was
. Temperature of greater than
affected the abnormal germination and germ tube elongation. The optimal relative humidity for germination and germ tube elongation was 85% and germination increased as relative humidity increased. Disease development started 8 days after inoculation and showed the highest disease severity at 15 days in greenhouse and field regardless of varieties. Among three different inoculation methods tested the spore dropping method was better than touching with infected leaves and spraying with spore suspension. However, the method has limitation in mass inoculation due to the amount of time consumed.
Regional Differences of Leaf Spot Disease on Grapevine cv. 'Campbell Early' Caused by Pseudocercospora vitis in Plastic Green House
Jung, Sung-Min ; Park, Jong-Han ; Park, Seo-Jun ; Lee, Han-Chan ; Lee, Jae-Wook ; Ryu, Myung-Sang ;
Research in Plant Disease, volume 15, issue 3, 2009, Pages 193~197
DOI : 10.5423/RPD.2009.15.3.193
Pseudocercospora leaf spot was major disease of grape cultivar 'Campbell Early' in Korea. Leaf spot first appeared in early June and rapidly dispersed to other leaves through rainy season. Disease progress of leaf spot by Pseudocercospora vitis in plastic green house, in the two provinces (Gimje and Gimcheon), were investigated in 2007. Differences of Infected leaves (%) between cultivation systems were observed in field and plastic green house, but there was no difference between provinces. Micro environmental factors, such as temperature and relative humidity, were correlated with infected leaves by PROC REG procedure of SAS (Statistical Analysis System). As a result, regression model best described (
) the infected leaves as a function of the interaction of cumulated temperatures; Y (Infected leaves)=-7.0101+0.0496
20Hcum (Cumulated hour above 20 degree)+0.0208
20cum (Cumulated temperature above 20 degree)-0.2781
25Hcum (Cumulated hour above 25 degree). A statistics model was shown that cumulated hour and temperature above specific degree were critical factor for Pseudocercospora leaf spot on the grapevine leaves in plastic green house.
Screening of Tomato Cultivars Resistant to Bacterial Wilts
Han, You-Kyoung ; Min, Ji-Seon ; Park, Jong-Han ; Han, Kyung-Sook ; Kim, Dae-Hyun ; Lee, Jung-Sup ; Kim, Hyeong-Hwan ;
Research in Plant Disease, volume 15, issue 3, 2009, Pages 198~201
DOI : 10.5423/RPD.2009.15.3.198
Bacterial wilts, caused by Ralstonia solanacearum, is a very destructive disease to tomato plants (Solanum lycopersicum) in Korea. Selection of resistant cultivar is the best way to prevent or reduce the occurrence of this disease. Thirty-nine tomato cultivars, twenty-one cherry tomato cultivars and thirteen rootstock tomato cultivars were inoculated with Ralstonia solanacearum, to evaluate tomato cultivars for resistance against bacterial wilts. Thirty-seven cultivars were highly susceptible to bacterial wilts and 61-100% of their whole tissue became wilted within 10 days after inoculation. Twenty-four cultivars showed moderate resistance and twelve were resistant to bacterial wilts. In an evaluation of 73 major commercial cultivars, 'Lilyance', 'TP-7', 'Choice', 'Dadaki', 'Akiko', 'Redstar', 'Match', 'B-blocking', 'Magnet', 'Support', 'Friend' and 'Special' were found to have a high level of resistance to bacterial wilts of tomatoes.
Evaluation of Disease Resistance of a Leaffolder-resistant (Cry1Ac1) Rice Event and Gene Transfer to Plant Pathogens
Nam, Hyo-Song ; Shim, Hong-Sik ; Yu, Sang-Mi ; Lee, Se-Won ; Kwon, Soon-Jong ; Kim, Myung-Kon ; Lee, Yong-Hoon ;
Research in Plant Disease, volume 15, issue 3, 2009, Pages 202~208
DOI : 10.5423/RPD.2009.15.3.202
The genetically modified leaffolder-resistant (Cry1Ac1) rice plant was evaluated for the changes of resistance by comparing the occurrence of major diseases with a japonica type Korean rice variety, Nakdong which was the mother plant of the transgenic rice event, in greenhouse and field conditions. There was no difference in the occurrence of sheath blight and Helminthosporium blight between the two varieties in the fields. We couldn't find any difference of resistance for fungal blast and bacterial leaf blight by artificial inoculation in greenhouse. There was also no difference in the susceptibility to sheath blight in artificial inoculation tests confirming the results in the fields. The possibility of gene transfer of Bar and Cry1Ac1 from the genetically modified rice plant to naturally infected pathogens such as Fusarium moniliforme and Pyricularia oryzae in the field conditions was tested by PCR. And the possible transfer of those genes by continuous inoculation of Xanthomonas oryzae pv. oryzae and Rhizoctonia solani was also tested. However, we couldn't find any possibility of transfer of the genes in natural and artificial conditions.
Physical Characteristics and Antagonistic Effect of Ampelomyces
Kim, Ji-Young ; Lee, Wang-Hyu ; Kim, Hyung-Moo ;
Research in Plant Disease, volume 15, issue 3, 2009, Pages 209~216
DOI : 10.5423/RPD.2009.15.3.209
During the period of June, 2005 to May, 2008, 44 host plants infected with powdery mildew were collected in the Jeon-ju and Jang-su districts of Jeonbuk province and in the Jang-sung district of Jeonnam province. The hyperparasites, Ampelomyces were confirmed in 12 plant species. Most of the pycnidium shapes of Ampelomyces were circular or oval shaped, and the sizes were different even within the same host plant, and also the color of pycnidium was ranged from light brown to dark brown. Ampelomyces species were isolated from 4 hosts including Impatiens balsamina L., Cucurbita pepo, Rudbeckia laciniata var. elatier and Youngia sonchifolia, and thus the most appropriate 12 Ampelomyces strains for the current experiment were selected. When analyzing the selected 12 strains' incubational and nutritional characteristics, the malt extract agar was the most appropriate media. When investigating the effect of osmotic pressure on the spore germination, 0.15M NaCl concentration was the optimum germination concentration. When the isolated Ampelomyces sp. was tested in-vitro, it was found to be effective to control in other plant pathogens, isolated Ampelomyces showed no pathogenicity to the plant. strains isolated . studied on rDNA ITS sequence analysis. The rDNA ITS sequence data of Ampelomyces sp. isolate BSLAH16 from Impatiens balsamina L. were analyzed and identified.
Control Efficacy of a New Fungicide Fludioxonil on Lettuce Gray Mold According to Several Conditions
Choi, Gyung-Ja ; Jang, Kyoung-Soo ; Choi, Yong-Ho ; Kim, Jin-Cheol ;
Research in Plant Disease, volume 15, issue 3, 2009, Pages 217~221
DOI : 10.5423/RPD.2009.15.3.217
Fludioxonil is derived from the antifungal compound pyrrolnitrin produced by Pseudomonas pyrrocinia and classified as a reduced-risk fungicide by the US EPA. The efficacy of fludioxonil for the control of lettuce gray mold caused by Botrytis cinerea was evaluated under several conditions such as growth stages of host, inoculum concentrations, and amounts of potato dextrose broth (PDB) included in spore suspension of B. cinerea. At 4-leaf stage of lettuce plants, fludioxonil applied at 2
/ml was more effective for the control of gray mold than at 5- and 6-leaf stages. However, fludioxonil at more than 10
/ml provided similar control activity in all growth stages of lettuce tested. The fungicide (10 and 50
/ml) also gave excellent control of gray mold on lettuce seedlings inoculated with spore suspensions of B. cinerea (
spores/ml). But, control efficacy of fludioxonil (2
/ml) was negatively correlated with inoculum concentration. Addition of PDB in spore suspension of B. cinerea resulted in higher disease severity than non-treated control. By inoculating spore suspension including 0.5% PDB, the fungicide gave the most control activity on the disease, followed by 1% and 2% PDB. The results suggest that fludioxonil has potential to control gray mold of lettuce, but the fungicide at a concentration having moderate activity may represent low control efficacy on the disease under some conditions.
Suppression Effect of Gray Mold and Late Blight on Tomato Plants by Rhamnolipid B
Ahn, Ji-Ye ; Park, Myung-Soo ; Kim, Seul-Ki ; Choi, Gyung-Ja ; Jang, Kyoung-Soo ; Choi, Yong-Ho ; Choi, Jae-Eul ; Kim, In-Seon ; Kim, Jin-Cheol ;
Research in Plant Disease, volume 15, issue 3, 2009, Pages 222~229
DOI : 10.5423/RPD.2009.15.3.222
A Pseudomonas strain SG3 producing biosurfactant and showing antifungal and insecticidal activities was isolated from agricultural soil severely contaminated with machine oils. The antagonistic bacterium inhibited mycelial growth of all of the tested fungal pathogens. The fermentation broth of SG3 also effectively suppressed the development of various plant diseases including rice blast, tomato gray mold, tomato late blight, wheat leaf rust, barley powdery mildew and red pepper anthracnose. An antifungal substance was isolated from the fermentation broth of SG3 by ethyl acetate partitioning, silica gel column chromatography and preparative HPLC under the guide of bioassay. The chemical structure of the antifungal substance was determined to be rhamnolipid B by mass and NMR spectral analyses. The antifungal biosurfactant showed a potent in vivo antifungal activity against gray mold and late blight on tomato plants. In addition, rhamnolipid B inhibited mycelial growth of B. cinerea causing tomato gray mold and zoospore germination and mycelial growth of P. infestans causing tomato late blight. Pseudomonas sp. SG3 producing rhamnolipid B could be used as a new biocontrol agent for the control of plant diseases occurring on tomato plants.
Effect of Skin Sooty and Decay Disease Control on ‘Niitaka’ Pear Fruit for Storage
Lee, Jung-Sup ; Choi, Jin-Ho ; Park, Jong-Han ; Kim, Dae-Hyun ; Han, Kyung-Sook ; Han, You-Kyoung ;
Research in Plant Disease, volume 15, issue 3, 2009, Pages 230~235
DOI : 10.5423/RPD.2009.15.3.230
Postharvest skin sooty dapple and decay disease of pear fruit often originates at small stain symptoms that occurred during harvest and handling. Experiments were conducted to characterize the effect of timing of application of disease control materials, and to evaluate sequential postharvest applications of fungicides or fungicides and bio-control agents. Fungicides and bio-control agents were increasingly less effective when the period between harvest and application was prolonged. Thiabendazole (TBZ) applied to fruit without artificial wounding or inoculation effectively reduced skin sooty and decay disease when applied within 3 weeks or 6 weeks in 2 years of study. TBZ, Fludioxonil and pyrimethanil were effective in controlling skin sooty and decay disease at artificial wounds inoculated with Cladosporium tenuissimum up to 14 days after inoculation. Application of TBZ at harvest followed 3 weeks later by application of Fludioxonil was superior to application of TBZ at harvest alone. Two bacterial biocontrol agents reduced skin sooty and decay disease at pear wounds inoculated with C. tenuissimum up to 14 days after inoculation with C. tenuissimum, but were ineffective when applied at 28 days after inoculation. Of possible sequential arrangements of fungicide and bio-control treatments, application of the most effective material promptly after harvest generally resulted in the highest level of disease control.
Environment Friendly Control of Gray Mold, a Ginseng Storage Disease Using Essential Oils
Kim, Jung-Bae ; Kim, Nam-Kyu ; Lim, Jin-Ha ; Kim, Sun-Ick ; Kim, Hyun-Ho ; Song, Jeong-Young ; Kim, Hong-Gi ;
Research in Plant Disease, volume 15, issue 3, 2009, Pages 236~241
DOI : 10.5423/RPD.2009.15.3.236
The objective of this study was to find an environment friendly method of ginseng storage disease control using a natural plant extract. Essential oil was evaluated in terms of its antifungal ability against a variety of ginseng storage pathogens, and a variety of essential oils was conducted in order to assess the possibility of applying them as a component of a disease control strategy. Direct treatment with essential oil was demonstrated to exert a ginseng storage control effect. Methyl eugenol and thymol were shown to exert a mycelial growth inhibition effect of 80% on PDA media, using a paper disc containing 200 ppm of essential oil against Botrytis cinerea. The application of direct methyl eugenol treatment to ginseng resulted in a profound control effect. Both spray and dipping treatment of each methyl eugenol as well as thymol, evidenced a disease develoment of 10-20% as compared with the over 80% observed from all non-treated packages. Methyl eugenol in the large packages resulted in a disease index of 0.60 in the two essential oil treatments and also a small diseased area, as compared with the disease index of 1.65 and the wide diseased area observed in the non-treatment groups. Treatment with a mixture (methyl eugenol + thymol) in the synergistic effect test resulted in a relatively wide diseased area, as no discernable synergistic effect was detected. Methyl eugenol and thymol can be utilized as control agents in an environmentally friendly ginseng storage treatment, owing to the avirulent and clear effects detected in this study. In particular, ginseng must be ingested when fresh, and this is why a product for the control of ginseng storage diseases is so necessary.
Effect of Crop Rotation on Control of Clubroot Disease of Chinese Cabbage Caused by Plasmodiophora brassicae
Kim, Jeom-Soon ; Lee, Jeong-Tae ; Lee, Gye-Jun ;
Research in Plant Disease, volume 15, issue 3, 2009, Pages 242~247
DOI : 10.5423/RPD.2009.15.3.242
To select rotation crops for control of clubroot of Chinese cabbage, potato, corn, soybean, onion and groundsel were planted in the field infected with clubroot pathogen (Plasmodiophora brassicae) in highland area in 2000. In comparison of economical efficiency among rotation crops, potato and onion gained about 16.9 and 14.9 times higher, respectively, than successive cultivation of Chinese cabbage. Resting spore densities of Plasmodiophora brassicae after harvesting rotation crops were in the range of
soil in all cultivated soils with rotation crops while that of successive Chinese cabbage cultivation soil was very high as much as
soil. And disease severity of Chinese cabbage clubroot was 4.9, 20.2, 24.4, 25.1 and 27.8% in onion, soybean, potato, corn, and groundsel cultivation plot, respectively, while that of successive Chinese cabbage cultivation plot was very high as 77.8%. Effect of rotation period of onion, potato, soybean on disease control was investigated from 2002 to 2005. Resting spore densities of Plasmodiophora brassicae after cultivating rotation crops were decreased until
year and maintained low density at
year in all plots, while that of successive Chinese cabbage cultivation plot was increased 2.6 to 23.6 times for three years. When Chinese cabbage was rotation-cultivated with potato, soybean and onion for three years, disease severities of Chinese cabbage clubroot decreased 92 to 4.4%, 72 to 10.4% and 72 to 12.2%, respectively, while that of successive Chinese cabbage cultivation plot maintained 100%. As the rotation period increased, the yields of Chinese cabbage increased, while that of successive Chinese cabbage cultivation plot decreased. At
year, Chinese cabbage with high quality could be much more produced 2,205, 2,493 and 2,476 g in potato, soybean and onion cultivation plot, respectively, than 95 g in successive Chinese cabbage cultivation plot.
Disease Control Efficacy of Chitosan Preparations against Tomato Leaf Mold
Chang, Tae-Hyun ;
Research in Plant Disease, volume 15, issue 3, 2009, Pages 248~253
DOI : 10.5423/RPD.2009.15.3.248
Chitosan has an antifungal activity and is widely used for control of various plant disease and plants growth in the field in Korea. Disease control efficacy of two preparations (SH-1, SH-2) of mixtures of high and low (chitooligosaccharide) molecular weight chitosan compounds against tomato leaf mold caused by Fulvia fulva was investigated under plastic greenhouse conditions. Both SH-1 and SH-2 formulations displayed potent disease control activity in two experiments. The protective activity of both preparations was comparable to synthetic thiophanate-M. The persistence activity of the formulations was sustained until 21 days after application. Effective concentration of the chtosan compounds for disease control was 1,200 mg a.i./L. In pot tests, chitosan preparations, at a concentration of 600 mg a.i./L, promoted plants growth. These results indicate that the chitosan preparations have a potential as an eco-friendly natural fungicide for the control of tomato leaf mold and plant growth regulator.
A Subgroup IB Isolate of Cucumber mosaic virus Isolated from Lagenaria leucantha var. gourda
Oh, Sun-Mi ; Hong, Jin-Sung ; Ryu, Ki-Hyun ; Lee, Gung-Pyo ; Choi, Jang-Kyung ;
Research in Plant Disease, volume 15, issue 3, 2009, Pages 254~258
DOI : 10.5423/RPD.2009.15.3.254
An isolate of Cucumber mosaic virus (CMV), called as Lag-CMV, was identified from Lagenaria leucantha var. gourda showing mosaic symptom, and its properties was compared to Fny-CMV (subgroup IA) and As-CMV (subgroup IB) by host reaction in several indicator plants, dsRNA analysis, RT-PCR analysis, restriction enzyme profile of the PCR products and nucleotide sequence of coat protein gene. Lag-CMV was similar to As-CMV used as a control CMV by the induced chlorotic spot on inoculated leaves and mosaic symptoms on upper leaves of N. tabacum. cv. Xanthi nc. In the cucumber and zucchini squash, Lag-CMV and As-CMV induced a mild mosaic symptoms than that of Fny-CMV. Size and shapes of local lesions on Chenophodium amaranticolor and Vigna unguiculata induced by Lag-CMV was similar those by Fny-CMV or As-CMV. In experiments of dsRNA profiles and RT-PCR analysis of coat protein gene, Lag-CMV was come within subgroup I CMV. Moreover, restriction enzyme analysis using EcoRI, SalI, MspI, XhoI, and HindIII of the RTPCR products and nucleotide sequence analysis of the coat protein gene showed that Lag-CMV belong to a member of CMV subgroup IB of the same to As-CMV.
Sclerotinia Shoot Rot of Grapevine (Vitis spp.) Caused by Sclerotinia sclerotiorum in Korea
Park, Jong-Han ; Han, Kyung-Sook ; Han, You-Kyoung ; Lee, Jung-Sup ; Kim, Dae-Hyun ; Hwang, Jeong-Hwan ;
Research in Plant Disease, volume 15, issue 3, 2009, Pages 259~261
DOI : 10.5423/RPD.2009.15.3.259
Sclerotinia shoot rot of Grapevine(Vitis labruscana) occurred at Gapyeong and Yeongwol area from 2003 to 2005. Infected plants showed shoot blight at the one-year-old fruit bearing branches. The first visible symptom noticed was wilting and blighting of the branches. The obvious and typical initial symptom is the presence of a cottony, white, dense mat of mycelial growth on the surface of the diseased lesions. The base or stem of the infected young shoots develop a pale brown rotted area, which girdled and killed shoots. In advanced stages of the disease, stems and branches became bleached and eventually died. The isolates collected from diseased grapevine stem were identified as Sclerotinia sclerotiorum based on the morphological and cultural characteristics. The pathogenicity test revealed that Vitis vinifera(cultivar: Kyoho) was stronger than V. labruscana(cultivar: Campbell early) to the pathogen. This is the first report on sclerotinia shoot rot of grapevine in Korea.
Transmission of Fusarium oxysporum by the Fungus Gnat, Bradysia difformis (Diptera: Sciaridae)
Kim, Hyeong-Hwan ; Jeon, Heung-Yong ; Yang, Chang-Yeol ; Kang, Taek-Joon ; Han, You-Kyoung ;
Research in Plant Disease, volume 15, issue 3, 2009, Pages 262~265
DOI : 10.5423/RPD.2009.15.3.262
The fungus gnat, Bradysia difformis was examined for its ability to transmit Fusarium oxysporum in PDA culture. Larvae and adults of B. difformis were able to transmit the fungus as ingested and sticking. We constructed GFP-expressed mutants with Fusarium oxysporum, then feed it to larvae of fungus gnat, B. difformis. So that mycelia were placed in the alimentary canal of larva.