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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Research in Plant Disease
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Journal DOI :
Korean Society of Plant Pathology
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Volume & Issues
Volume 16, Issue 3 - Dec 2010
Volume 16, Issue 2 - Aug 2010
Volume 16, Issue 1 - Apr 2010
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Analysis of the Factors Involved in the Occurrence of Rice Stripe virus in Chungcheongbukdo in 2008 and 2009
Kang, Hyo-Jung ; Ahn, Ki-Su ; Han, Chong-U ; Jeong, Kyeong-Heon ; Park, See-Jung ; Ji, Jae-Jun ; Kim, Jeong-Soo ;
Research in Plant Disease, volume 16, issue 2, 2010, Pages 109~114
DOI : 10.5423/RPD.2010.16.2.109
The occurrence factors of Rice stripe virus (RSV) in Chungcheongbukdo were analyzed by investigating the viruliferous insect rate (VIR) of overwintered small brown plant hopper (SBPH), the population density of SBPH, the infection rate of natural host plants, and the occurrence rate of RSV on rice paddy fields at the 3 areas of Cheongwon, Jincheon, and Boeun in 2008 and 2009. The average VIR of overwintered SBPH was 0.0% in 2008 and 1.1% in 2009. From SBPH collected on early June in 2009, VIR was higher as 1.4% at Jincheon and 4.2% at Boeun than those of overwintered SBPH, and this higher VIR might relate stronlgy with the adult population of SBPH immigrated from China. The populations of SBPH at Cheongwon, Jincheon and Boeun in 2008 were 3.8, 7.5 and 20.8 Head/
, respectively. However, those of Cheongwon and Jincheon increased up to about two folds as 8.4 and 13.1 in 2009. No RSV was detected on the natural host plants including barley. The factors involved in RSV occurrence were affected negatively by the low VIR of overwintered SBPH, the low population of overwintered SBPH, the low infection rate of RSV on the natural host plants, and the clean cultivation environment in Chungcheongbukdo.
Effect of Cropping System on Disease Incidence by Soil-borne Bymovirus in Barley and on Density of the Vector, Polymyxa graminis
Park, Jong-Chul ; Noh, Tae-Hwan ; Kim, Mi-Jung ; Lee, Sang-Bok ; Park, Chul-Soo ; Kang, Chun-Sik ; Lee, Jung-Joon ; Kim, Tae-Soo ;
Research in Plant Disease, volume 16, issue 2, 2010, Pages 115~120
DOI : 10.5423/RPD.2010.16.2.115
In this study, changes in virus disease occurrence and yield were monitored in conventional cropping system(rice-barley) and soybean-barley double cropping system in virus-prone area for 5 years. Also, changes in the density of Polymyxa graminis, a fungal vector, was investigated. In assay tests, mixed infection of Barley yellow mosaic virus (BaYMV) and Barley mild mosaic virus (BaMMV) was observed. Disease severity was in the range of 7~9 in conventional cropping system. In continuous cropping of soybean-barley and 3-yearfallow land, disease severity also was around 7. However, disease severity was reduced to medium level (5) when barley cultivation was paused for one or two years in soybean-barley cropping. When barley cultivation was paused for a year, the density of P. graminis, a fungal vector for BaYMV and BaMMV, reduced in barley root and soil. Similarly, barley growth was also enhanced by adopting fallow seasons. Compared with the fifth year of conventional cropping, the number of tillers per
was increased by 158 when barley cultivation was paused for an year in soybean-barley cropping. When soybean and barley were cultivated continuously or complete fallow period was extended to three years, plant height and the number of tillers of barley were decreased. Yield components of barley in soybean-barley cropping were superior to those in rice-barley cropping. Compared with the fifth year of conventional cropping and soybean-barley cropping, culm length of barley was 1.3~2.3 cm higher and the number of tillers per
was 36~90 higher when barley cultivation was paused for one or two years. However, those in continuous cropping of soybean-barley and 3-year-fallow land were lower compared with conventional cropping. Similarly, yield was increased when barley cultivation was paused for one or two years in the third, forth, and fifth years when compared with conventional cropping.
Immunocapture RT-PCR for Detection of Seed-borne Viruses on Cucurbitaceae Crops
Lee, Hyok-In ; Kim, Jung-Hee ; Yea, Mi-Chi ;
Research in Plant Disease, volume 16, issue 2, 2010, Pages 121~124
DOI : 10.5423/RPD.2010.16.2.121
Immunocapture reverse transcription polymerase chain reaction (IC-RT-PCR) was applied to the detection of Cucumber green mottle mosaic virus (CGMMV), Kyuri green mottle mosaic virus (KGMMV), and Zucchini green mottle mosaic virus (ZGMMV) on Cucurbitaceae crops. These seed-borne tobamoviruses were accurately detected from the infected leaves and seeds by IC-RT-PCR. This method was estimated to be about 100 times more sensitive than ELISA, and also it allowed the direct confirmation of ELISA results by using the captured antigens from a completed ELISA microwell. This convenient and reliable method could be used routinely for large-scale field surveys or seed tests of Cucurbitaceae crops.
PCR Primer Developed for Diagnosis of Xanthomonas arboricola pv. pruni in Prune
Ryu, Young-Hyun ; Lee, Joong-Hwan ; Kwon, Tae-Young ; Kim, Seung-Han ; Kim, Dong-Geun ;
Research in Plant Disease, volume 16, issue 2, 2010, Pages 125~128
DOI : 10.5423/RPD.2010.16.2.125
Bacterial black spot disease of prune fruit (Prunus salicina cv. formosa) has outbroke around major prune production area, Gimcheon, Euiseong and Gunwi in Gyungbuk province and has caused severe economic loss. Integrons PCR primer was designed along with sample pre-incubation and nested PCR method to enhance detection sensitivity for early detection of bacteria in fields. Designed integrons PCR primer successfully detected Xanthomonas arboricola pv. pruni from field collected samples, fruit, leaf, branch and even in raindrop collected from prune orchard. Pre-incubation along with nested PCR enhanced sensitivity to detect X. arboricola pv. pruni from seemingly healthy looking, symptomless branches. Designed integrons PCR can be used in prune nursery fields and in plant quarantine practice for the detection of X. arboricola pv. pruni.
Development of PCR Primers to Detect Pseudomonas savastanoi pv. phaseolicola from the Bean Seeds
Cho, Jung-Hee ; Jeong, Min-Jung ; Song, Min-Ji ; Yim, Kyu-Ock ; Lee, Hyok-In ; Kim, Jung-Hee ; Baeg, Ji-Hyun ; Cha, Jae-Soon ;
Research in Plant Disease, volume 16, issue 2, 2010, Pages 129~135
DOI : 10.5423/RPD.2010.16.2.129
PCR primers were developed to detect Pseudomonas savastanoi pv. phaseolicola, a causal agent of halo blight that occurs in all species of common bean (Phaseolus vulgaris L.), from the bean seeds. A primer set, Psp-JHF and Psp-JH-R, specifically amplified 513 bp fragment from Pseudomonas savastanoi pv. phaseolicola only. A nested primer set, psp-JH-F-ne and psp-JH-R-ne, designed from the
PCR amplicon, amplified 169 bp fragment. The primer sets did not amplify any non-specific DNA from the seed extracts of Fabaceae including 4 beans, 2 soybeans, and 2 peas. The detection sensitivity of the nested PCR method developed in this study was much higher than that of ELISA and selective medium. PCR assays developed in this study should be useful to detect Pseudomonas savastanoi pv. phasolicola from the bean seeds.
Suppression Effect on Soft-rot by Bacteriocin-producing Avirulent Pectobacterium carotovorum subsp. carotovorum Pcc21-M15
Roh, Eun-Jung ; Lee, Seung-Don ; Heu, Sung-Gi ;
Research in Plant Disease, volume 16, issue 2, 2010, Pages 136~140
DOI : 10.5423/RPD.2010.16.2.136
Pectobacterium carotovorum subsp. carotovorum causes soft rot disease in diverse plants. Carocin D is bacteriocin that is produced by Pectobacterium carotovorum subsp. carotovorum Pcc21 strain. Nonpathogenic mutant P. carotovorum subsp. carotovorum Pcc21-M15 strain was obtained by mutagenesis with Tn5 insertion and screened pathogenesity. P. carotovorum subsp. carotovorum Pcc21-M15 and E. coli (pRG3431), carocin D gene-transformed E. coli, produce carocin D against P. carotovorum subsp. carotovorum Pcc3. Pathogenic P. carotovorum subsp. carotovorum Pcc3 and mixture with Pcc21-M15 or E. coli (pRG3431) were treated with lettuces. Pcc21-M15 and E. coli (pRG3431) effectively suppressed the development of soft-rot disease. While symptoms in 90% of Pcc3-treated lettuces were observed after 3 days, only 25% of Pcc3 and Pcc21-M15-treated lettuces were observed to be infected after 6 days. These results suggest that the nonpathogenic strain P. carotovorum subsp. carotovorum. Pcc21-M15 and E. coli (pRG3431) are effective to soft-rot disease suppression.
Resistance to Phytophthora Blight of Commercial Pepper Cultivars in Korea
Kim, Byung-Soo ; Kwon, Tae-Ryong ; Hwang, Ji-Eun ; Lee, Jae-Moo ; Park, Dong-Guen ; Ahn, Joon-Hyung ; Kim, Hye-Yeon ;
Research in Plant Disease, volume 16, issue 2, 2010, Pages 141~147
DOI : 10.5423/RPD.2010.16.2.141
Resistance to Phytophthora blight (Phytophthora capsici Leonian) of commercial cultivars of Capsicum pepper in Korea were evaluated from 2008 to 2010. In 2008 and 2009, the cultivars were tested for resistance to the Pc003 isolate collected in Youngyang, Gyeongbuk province. In 2010, the selected cultivars in the previous years were tested by inoculating with 3 isolates obtained from the pepper plants in Miryang in Gyeongnam province, Youngyang in Gyeongbuk province, and Goesan in Chungbuk province. A continuous variation in resistance from highly resistant to susceptible was observed among the commercial pepper cultivars. It was also noted that some cultivars, although their names were initiating with `Yeokgang` or `PR` meaning Phytophthora resistance, were very low in resistance or susceptible. When the resistant cultivars selected in 2008 and 2009 experiment were inoculated with the 3 isolates, all the commercial cultivars except a rootstock, `Tantan`, succumbed to the exceptionally virulent Pc005 (Miryang) isolate. Pc002 (Goesan) was a little more virulent than Pc003 (Youngyang). A few cultivars resistant to Pc003 (Youngyang) were severely infected by Pc002 (Goesan). Significant interaction in analysis of variance suggested the differential interactions between cultivars and pathogen isolates. Strategies to breed cultivars having high level of resistance to the highly variable pathogenic fungus, Phytophthora capsici, were discussed.
Development of Effective Screening Method and Evaluation of Radish Cultivars for Resistance to Fusarium oxysporum f. sp. raphani
Baik, Song-Yi ; Kim, Jin-Cheol ; Jang, Kyoung-Soo ; Choi, Yong-Ho ; Choi, Gyung-Ja ;
Research in Plant Disease, volume 16, issue 2, 2010, Pages 148~152
DOI : 10.5423/RPD.2010.16.2.148
To establish the efficient screening method for resistance of radish to F. oxysporum f. sp. raphani, we investigated the development of Fusarium wilt of two radish cultivars, `Songbaek` (susceptible) and `Tokwang` (moderately resistant), according to several conditions such as inoculation methods, inoculum concentrations, and dipping periods of radish roots in spore suspension. By infected soil and soil-drenching inoculation methods, Fusarium wilt did not occur on the seedlings of both cultivars. In root dipping inoculation method using cut or non-cut roots of radish plants, the cut roots were easily infected by the pathogen than non-cut roots. And the disease development of two cultivars represented significant difference in non-cut root method. On the other hand, disease severity of Fusarium wilt on radish seedlings according to inoculum concentration increased in a dose-dependant manner, regardless of dipping periods. Using screening method established from the results, the 41 commercial radish cultivars were evaluated the degree of resistance to F. oxysporum f. sp. raphani. Among them, 6 radish cultivars were resistant, 22 cultivars were moderately resistant, and 13 cultivars were susceptible to Fusarium wilt.
The Race Differentiation of Phytophthora capsici in Korea
Lee, Sang-Jun ; Park, Yong-Ju ; Kim, Heung-Tae ; Kim, Byung-Sup ;
Research in Plant Disease, volume 16, issue 2, 2010, Pages 153~157
DOI : 10.5423/RPD.2010.16.2.153
This study was examined to identify the race differentiation and distribution of mating type on Phytophthora capsici population in Korea. One hundred forty three isolates of P. capsici were collected from several locations of Korea in 2005-2007. In 2005, 20 isolates of P. capsici were collected and surveyed as A1 mating type of 75% and A2 mating type of 25%. In 2006, a total of 91 isolates were collected and separated as A1 mating type of 49.0%, A2 mating type of 42.9% and S type (sterile) of 3.3%. Isolates obtained in 2007 were similar to 2006 results. Totally, ratio of mating type of 153 isolates was confirmed that A1 type was 56.6%, A2 type was 39.2%, and S type was 4.2%. Thirteen pepper cultivars with different pathogenic response to 3 typical isolates having different mating were screened among 50 pepper cultivars and determined as race differential cultivars for investigation. The 11 races of P. capsici were found by using 13-race differential cultivars. These results indicated that at least 11 races of P. capsici are existed and confirmed race differentiation of P. capsici in pepper.
Selection of Biocontrol Agents against Phytophthora Blight of Pepper and Its Root Colonization Ability
Zhang, Li-Jing ; Shi, Hong-Zhong ; Wang, Jing-Jing ; Chang, Shu-Xian ; Shen, Shun-Shan ;
Research in Plant Disease, volume 16, issue 2, 2010, Pages 158~162
DOI : 10.5423/RPD.2010.16.2.158
Four promising biocontrol agents against Phytophthora capsici were selected from 507 bacterial isolates collected from rhizosphere soils and roots of pepper plants. In vitro experiment, these four biocontrol agents inhibited mycelial growth, germination of cystospores, and formation of zoosporangia and zoospores of Phytophthora capsici. In the pot experiment, the four biocontrol agents showed control efficiency higher than 70%. In greenhouse experiment, the isolates G28-6 gave the control value of 79.4%. These four biocontrol agents successfully colonized in the population density beyond 105 cfu/g on roots of pepper in vitro. The isolates G28-6 was identified as Pseudomonas aurantiaca, based on its cultural, morphological, and biochemical characterization and 16S rRNA gene sequence analysis.
Elucidation of Mode of Action of Pantoea agglomerans 59-4 for Controlling Garlic Blue Mold
Kim, Yong-Ki ; Kwon, Mi-Kyung ; Yeh, Wan-Hae ; Hong, Sung-Jun ; Jee, Hyung-Jin ; Park, Jong-Ho ; Han, Eun-Jung ; Park, Kyung-Seok ; Lee, Sang-Yeob ; Lee, Seong-Don ;
Research in Plant Disease, volume 16, issue 2, 2010, Pages 163~169
DOI : 10.5423/RPD.2010.16.2.163
To screen for potential biocontrol agents against postharvest disease of garlics caused by Penicillium hirsutum, a total of 1292 isolates were isolated from the rhizoshere or rhizoplane of Allium species. Among them, S59-4 isolate was selected as a potential biocontrol agent by in vivo wounded garlic bulb assay. The isolate was identified as Pantoea agglomerans (Pa59-4) through Biolog system. Pa59-4 did not inhibit the mycelial growth of P. hirsutum in dual-culture with P. hirsutum on tryptic soy agar. In order to elucidate mode of action of Pa59-4 on biological control, nutrient competition between Pa59-4 and P. hirsutum was investigated by the simple method using tissue culture plates with cylinder inserts containing defusing membrane reported by Janisiewicz et al. (2000). The results showed that Pa59-4 effectively suppressed spore germination and mycelial growth of blue mold in the low concentration (0.5%) of garlic juice, but it did not suppress those of blue mold in the high concentration (5%) of garlic juice. This result suggests that the mechanism in biocontrol of garlic blue mold by Pa 59-4 may be involved in nutrient competition with P. hirsutum on garlic bulbs.
Control Effects of Benzylideneacetone Isolated from Xenorabdus nematophilla K1 on the Diseases of Redpepper Plants
Park, Su-Jin ; Jun, Mi-Hyun ; Chun, Won-Su ; Seo, Ji-Ae ; Yi, Young-Keun ; Kim, Yong-Gyun ;
Research in Plant Disease, volume 16, issue 2, 2010, Pages 170~175
DOI : 10.5423/RPD.2010.16.2.170
A monoterpenoid benzylideneacetone (BZA) is a bacterial metabolite isolated from culture broth of an entomopathogenic bacterium, Xenorhabdus nematophila K1. It was tested in this study the control efficacy of the metabolite against two major fungal diseases occurring in red-pepper plants. BZA exhibited significant antifungal activities against Phytophthora capsici and Colletotrichum acutatum. Under natural light conditions, the antifungal activity of BZA was maintained for more than sixty days. The antifungal activity of BZA was not lost even in soil because the incidence of Phytophthora blight against red-pepper plants was significantly reduced when the suspensions of P. capsici were poured to the rhizosphere soils mixed with BZA. Application of the BZA suspension spray to the fruit surface infected with C. acutatum significantly suppressed the disease occurrence of anthracnose on the red-pepper plants. These results suggest that BZA can be used to develop a promising agrochemical to control phytophthora blight and anthracnose of redpepper plants.
Evaluation of Disease Occurrence by Cultivar, Sowing Date and Locational Difference in Korean Soybean Fields
Kim, Hong-Joe ; Oh, Ji-Yeon ; Kim, Dong-Kwan ; Yun, Hong-Tai ; Jung, Woo-Suk ; Hong, Jeum-Kyu ; Kim, Ki-Deok ;
Research in Plant Disease, volume 16, issue 2, 2010, Pages 176~182
DOI : 10.5423/RPD.2010.16.2.176
Occurrence of plant diseases is dependent on various factors in the agricultural system. Due to recent extensive environmental climate changes, i.e. global warming, agricultural systems such as planting dates and cultivars are being affected. Gradual transition in disease occurrence and incidence in the agricultural fields can also be affected by direct and/or indirect environmental changes. In this study, we evaluated disease occurrence and incidence in soybean plants to investigate whether it could be related with cultivars, planting dates and geographical differences in Korea in 2008. Soybean cultivars including `Taekwang`, `Pungsan`, `Cheongja 3`, `Saeol`, and `Dawon` were planted in four different dates, May 15, June 1, June 15, and June 30, in two locations, Suwon, and Naju. Soybean diseases such as wild fire and bacterial pustule were mainly found depending on cultivars, planting dates, and areas. Wild fire occurred severely on cv. `Taekwang` while bacterial blight did on cv. `Dawon` among tested cultivars. Disease developments of wild fire and bacterial blight generally decreased in delayed planting regardless of cultivars.
Survey of Major Disease Incidence of Rice in Korea during 1999-2008
Lee, Yong-Hwan ; Ra, Dong-Soo ; Yeh, Wan-Hae ; Choi, Hyo-Won ; Myung, Inn-Shik ; Lee, Se-Won ; Lee, Yeong-Hoon ; Han, Sung-Suk ; Shim, Hong-Sik ;
Research in Plant Disease, volume 16, issue 2, 2010, Pages 183~190
DOI : 10.5423/RPD.2010.16.2.183
During the period from 1999 to 2008, we surveyed the major diseases incidence of rice in Korea. Leaf blast showed 0.85% of the diseased leaf area in 2000 and then consistently decreased to 0.02% by 2008. However, panicle blast irregularly appeared by the years depending on weather conditions for disease development from heading to milk stage of rice. Diseased lesion height of sheath blight had the lowest (10.1%) incidence in 2001 and the highest (21.4%) incidence in 2007. A negative corelation ($r