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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Research in Plant Disease
Journal Basic Information
Journal DOI :
Korean Society of Plant Pathology
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Volume & Issues
Volume 18, Issue 4 - Dec 2012
Volume 18, Issue 3 - Sep 2012
Volume 18, Issue 2 - Jun 2012
Volume 18, Issue 1 - Apr 2012
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A Duplex PCR for Detection of Phytophthora katsurae Causing Chestnut Ink Disease
Lee, Dong-Hyeon ; Lee, Sun-Keun ; Kim, Hye-Jeong ; Lee, Sang-Hyun ; Lee, Sang-Yong ; Lee, Jong-Kyu ;
Research in Plant Disease, volume 18, issue 2, 2012, Pages 73~79
DOI : 10.5423/RPD.2012.18.2.073
Phytophthora katsurae is a fungal pathogen responsible for chestnut ink disease. We designed two duplex primer sets (SOPC 1F/1R+KatI 3F/5R, SOPC 1-1F/1-1R+KatI 3F/5R) to detect P. katsurae. SOPC 1F/1R and SOPC 1-1F/1-1R primer pairs were designed for sequence characteristic amplification regions (SCAR) marker, and KatI 3F/5R primer pair was used for P. katsurae-specific primer designed from internal transcribed spacer (ITS) region. To assess the sensitivity of duplex PCR, genomic DNA was serially diluted 10-fold to make the final concentrations from 1 mg/ml to 1 ng/ml. The sensitivity for two primer sets were 1
and 100 ng/ml, respectively. To find detection limits for zoospores of P. katsurae, each zoospore suspension was serially diluted 10-fold to make the final concentrations from
cells/ml, and then DNA was extracted. The limits of detection for all of two primer sets were
cells/ml. All of two primer sets were specific to P. katsurae in PCR detection and did not produce any P. katsurae-specific PCR amplicons from other 16 Phytophthora species used as the control. This study shows that duplex PCR using two primer sets might be a useful tool for rapid and efficient detection of P. katsurae.
Comparative Analysis between Healthy and Powdery Mildew-infected Plants of Strawberry Cultivar Seolhyang
Nam, Myeong-Hyeon ; Jeon, Yi-Nae ; Lee, Hee-Chul ; Lee, He-Duck ; Kang, Hee-Kyoung ;
Research in Plant Disease, volume 18, issue 2, 2012, Pages 80~85
DOI : 10.5423/RPD.2012.18.2.080
We examined the incidence of powdery mildew in strawberry cv. Seolhyang plants, and performed a comparative analysis on the temperature, photosynthesis rate, and nutrient content of healthy and powdery mildew-infected plants. Powdery mildew first infected the fruit of the strawberries in mid-January, and the disease severity increased in both fruits and leaves during the late harvest season. The rate of photosynthesis and leaf temperatures of healthy plants were higher than those of powdery mildew-infected leaves and significantly decreased with an increase in the disease severity. The healthy and powdery mildew-infected plants in the soil analysis were not significantly different in chemical compositions. The leaves of the healthy plants contained lower potassium and higher manganese and chlorophyll concentrations than the powdery mildew-infected plants. In particular, manganese was significantly higher in healthy leaves than in infected leaves. Therefore, the concentrations of potassium, manganese and chlorophyll in strawberry leaves may be an important factor for disease suppression.
Development of Efficient Screening Method for Resistant Cabbage and Broccoli to Plasmodiophora brassicae
Jo, Su-Jung ; Shim, Sun-Ah ; Jang, Kyoung-Soo ; Choi, Yong-Ho ; Kim, Jin-Cheol ; Choi, Gyung-Ja ;
Research in Plant Disease, volume 18, issue 2, 2012, Pages 86~92
DOI : 10.5423/RPD.2012.18.2.086
Clubroot caused by Plasmodiophora brassicae Woron. is one of the most important diseases in Brassica crops worldwide. To establish more simple and reliable screening method for resistant cabbage and broccoli to P. brassicae, the development of clubroot on the plants according to inoculum concentration and incubation period after inoculating with the pathogen was investigated using P. brassicae GN1 isolate (race 9). To facilitate and acquire precise result of resistance screening of cabbage and broccoli to clubroot, 14-day-old seedlings were inoculated by drenching roots with the spore suspension of P. brassicae to give inoculum density of
spores/pot. To develop the disease, the inoculated seedlings were incubated in a growth chamber at
for 3 days, and then cultivated in a greenhouse (
) for five weeks. Under the optimum conditions, 16 cabbage and 17 broccoli cultivars were tested for resistance to four field isolates (GN1, GN2, GS and YC) of P. brassicae collected from four regions in Korea. Among them, some cabbage and broccoli cultivars showed different resistance response to three isolates (GN1, GN2 and GS) determined as race 9 by using the differential varieties of Williams. On the other hand, all the tested cultivars were highly susceptible to YC isolate (race 2). The results suggest that this method is efficient screening method of cabbage and broccoli for resistance to P. brassicae.
Evaluation of Resistance to Colletotrichum acutatum in Pepper Genetic Resources
Kim, Sang-Gyu ; Ro, Na-Young ; Hur, On-Sook ; Ko, Ho-Cheol ; Gwag, Jae-Gyun ; Huh, Yun-Chan ;
Research in Plant Disease, volume 18, issue 2, 2012, Pages 93~100
DOI : 10.5423/RPD.2012.18.2.093
Resistance of pepper (Capsicum spp.) to anthracnose (Colletotrichum acutatum) was evaluated during regeneration of Capsicum spp. in National Agrobiodiversity Center. Disease severity of 896 pepper accessions (430 accessions of C. annuum, 219 accessions of C. baccatum, 14 accessions of C. chacoense, 153 accessions of C. chinense, 70 accessions of C. frutescens, 2 accessions of C. pubescens, and unidentified 8 accessions) was investigated at 14 days after inoculation in
humid chamber. Forty nine accessions of pepper germplasm were resistant to C. acutatum. Among them, nine accessions were highly resistant to C. acutatum without wounding spray inoculation. Four accessions belonged to the species C. baccatum, one accession to C. chacoense, and four accessions to C. frutescens. Forty two resistant candidate accessions were inoculated with pin-prick wounding using a syringe needle. Five accessions were resistant as a less than 3% of disease severity to C. acutatum with wounding inoculation 5 days after inoculation. All resistant accessions were C. baccatum. These five pepper germplasm might be used as breeding resources for the anthracnose resistance breeding program.
Distribution of Bursaphelenchus xylophilus in Naturally Infected Pinus densiflora and P. koraiensis and Migration of B. xylophilus in Artificially Inoculated P. densiflora Seedlings
Kim, Jae-Geun ; Kim, Byung-Kwan ; Lee, Seung-Kyu ; Kim, Jin-Cheol ; Han, Sang-Sub ; Cha, Byeong-Jin ;
Research in Plant Disease, volume 18, issue 2, 2012, Pages 101~108
DOI : 10.5423/RPD.2012.18.2.101
In 2006, pinewood nematode, Bursaphelenchus xylophilus, was isolated from about 50 years old trees of Pinus densiflora and P. koraiensis showing leaf-wilt and -drying symptoms in Gwangju, Gyeonggi-do and Chuncheon, Gangwon-do. Isolation of pinewood nematodes from sapwood of infected pine trees showed no difference in population density between tree species and among the sampling heights on the main stem. Migration of pinewood nematodes in the host tree were investigated by inoculation of red pine (P. densiflora, 3 years old) seedlings with B. xylophilus. The nematodes seemed to move in red pine seedlings prior to multiplication and it might have taken about 20 days to start multiplication and expression of symptoms including wilt and dieback. In initial time after inoculation, nematodes started migration through the cortical resin canal from inoculated site and further showed upward and downward movements. More nematodes were observed in cortical resin canal during early period of inoculation and later in resin canal of xylem and tracheid also while, the pith still remained free from nematode. The density of B. xylophilus was higher in seedlings of low-vigor with poor root growth than in seedlings of normal root growth. Seedlings showing high density of B. xylophilus exhibited stem discoloration and secondary infection by fungus at the inoculation site.
Antifungal Activity of Benzoic Acid from Bacillus subtilis GDYA-1 against Fungal Phytopathogens
Yoon, Mi-Young ; Seo, Kook-Hwa ; Lee, Sang-Heon ; Choi, Gyung-Ja ; Jang, Kyoung-Soo ; Choi, Yong-Ho ; Cha, Byeong-Jin ; Kim, Jin-Cheol ;
Research in Plant Disease, volume 18, issue 2, 2012, Pages 109~116
DOI : 10.5423/RPD.2012.18.2.109
A bacterial strain antagonistic to some fungal phytopathogens was isolated from the stem of a Persimmon tree in Yeongam, Korea. This bacterium was identified as Bacillus subtilis by 16S rRNA gene sequencing and designated as B. subtilis GDYA-1. In in vivo experiment, the fermentation broth exhibited antifungal activities against Magnaporthe oryzae on rice plants, Phytophthora infestans on tomato plants, and Puccinia recondita on wheat plants. We isolated one antifungal compound and its chemical structure was determined by mass and
-NMR spectral data. The antifungal substance was identified as benzoic acid. It inhibited mycelial growth of M. oryzae, Rhizoctonia solani, Sclerotinia sclerotiorum, and P. capsici with minimum inhibition concentration (MIC) values, ranging from 62.5 to 125
. Moreover, the substance effectively suppressed Phytophthora blight of red pepper caused by P. capsici in a pot experiment. To the author's knowledge, this is the first report on the antifungal activity of benzoic acid against phytopathogenic fungi. Benzoic acid and B. subtilis GDYA-1 may contribute to environmental-friendly protect crops from phytopathogenic fungi.
Control of Common Scab of Potato Caused by Streptomyces spp. by Soil pH Adjustment and Crop Rotation
Kim, Jeom-Soon ; Lee, Young-Gyu ; Kwon, Min ; Kim, Ju-Il ; Lee, Gye-Jun ; Lee, Jeong-Tae ; Ryu, Jong-Soo ;
Research in Plant Disease, volume 18, issue 2, 2012, Pages 117~122
DOI : 10.5423/RPD.2012.18.2.117
In 2006, the effect of soil pH adjustment on control of common scab of potato caused by Streptomyces spp. was evaluated in the field. Soil with an original pH 5.7 was treated with sulfur of 42 kg/10a and calcium hydroxide of 81.6 kg/10a and 184.5 kg/10a to adjust pH 5.0, 6.0 and 6.5, respectively. Additionally, the effect of gypsum (522 kg/10a) on soil pH and disease severity was examined. The soil pH of sulfur treatment plot was continuously declined from 5.13 at tuber initiation time to 5.01 at harvest. The disease severity of sulfur treatment plot was relatively low (22.8%) and the marketable yield of that was high (90.5%). By contrast, the soil pHs treated with calcium hydroxide (81.6 kg/10a and 184.5 kg/10a) were increased (pH 6.06 and 6.49, respectively) at harvest. In addition, calcium hydroxide treatment plots showed higher disease severities (51.0% and 61.1%), and lower marketable yields (55.7% and 37.0%). Meanwhile, the soil pH of gypsum treatment plot was not changed until harvest, and the disease severity and the marketable yield were not significantly different from those of control. The effect of crop rotation was also evaluated with four crops such as welsh onion, soybean, corn and Chinese cabbage. These crops were planted in the infested field from 2005 to 2007, and potatoes were planted in the same field in 2008. The disease severities of potatoes produced after rotations with welsh onion, soybean, corn and Chinese cabbage for 3 years were 13.1%, 16.7%, 28.9% and 30.2%, respectively. However, the disease severity of 73.1% was shown in continuous cropping of potato for 3 years. In addition, similar effects were exhibited in the marketable yield. These results demonstrate that the adjustment of soil pH and crop rotation might be very useful tools to control common scab of potato.
Screening of Resistance Cultivar to Clubroot Caused by Plasmodiophora brassicae for Organic Cultivation of Chinese Cabbage
Kim, Min-Jeong ; Shim, Chang-Ki ; Kim, Yong-Ki ; Hong, Sung-Jun ; Park, Jong-Ho ; Han, Eun-Jung ; Lee, Min-Ho ; Jee, Hyeong-Jin ;
Research in Plant Disease, volume 18, issue 2, 2012, Pages 123~128
DOI : 10.5423/RPD.2012.18.2.123
We investigated the resistance of 50 commercial Chinese cabbage cultivars against clubroot disease caused by Plasmodiophora brassicae in the three difference fields, Suwon, Hwacheon, and Pyeongchang. Wilting symptom on Chinese cabbage was first observed at 15 days after transplanting in Pyeongchang and Hwacheon, while disease symptoms appeared later in Suwon after the rainy season. Among 50 cultivars, eight cultivars, SC26, SC29, SC30, SC31, SC34, SC46, SC47 and SC50 showed highly susceptible symptoms like wilting and heavy root galls in all three fields. Meanwhile, seven cultivars such as SC05, SC06, SC07, SC09, SC11, SC17, and SC36 showed moderate resistance with delayed wilting and few root galls. Only two cultivars, Chuwol (CB22) and Gohyangssam (CB23) were highly resistant to clubroot disease until the harvest season in all of the three fields. These two commercial cultivars may be considered as candidate cultivars for cultivation of organic Chinese cabbage in Suwon, Hwacheon, and Pyeongchang.
Detection of Xanthomonas axonopodis pv. aurantifolii and Xanthomonas axonopodis pv. citrumelo by Triplex PCR
Yu, Sang-Mi ; Lee, Se-Won ; Lee, Seung-Don ; Park, Eun-Woo ; Lee, Yong-Hoon ;
Research in Plant Disease, volume 18, issue 2, 2012, Pages 129~132
DOI : 10.5423/RPD.2012.18.2.129
Citrus bacterial canker is an economically important disease affecting citrus production in many citrusgrowing areas and several pathotypes have been recognized within the Xanthomonas pathogens causing canker. In view of the containment of the disease, accurate identification of the causal bacterium is important. In this study, triplex PCR method was developed by using the previously reported primers. Two groups of primer combination, such as, one group including primers 2/3, J-pth1/J-pth2 and XACF/XACR, and another group 2/3, J-pth1/J-pth2 and Xac01/Xac02, were suitable for the detection and differentiation of X. a. pv. citri
, X. a. pv. aurantifolii B and C, and X. a. pv. citrumelo E strains. Moreover, the primer combination of Xac01 and J-pth2 promised us to use as a specific primer set to detect X. a. pv. citrumelo E strain. The PCR methods developed in this study could be used for the rapid differentiation of Xanthomonas pathotypes of citrus.
Occurrence of Powdery Mildew Caused by Erysiphe abeliicola on Glossy Abelia in Korea
Cho, Sung-Eun ; Park, Ji-Hyun ; Lee, Seung-Kyu ; Lee, Sang-Hyun ; Shin, Hyeon-Dong ;
Research in Plant Disease, volume 18, issue 2, 2012, Pages 133~138
DOI : 10.5423/RPD.2012.18.2.133
In November 2009, a powdery mildew on glossy abelia (Abelia
grandiflora) was found in Seogwipo, Jeju Island, Korea. Further survey in the southern part of Korea, e.g., Jeju, Busan, and Tongyeong confirmed occurrence of the disease. White colonies were present on leaves, young stems, and flowers, detracting from their beauty in landscape plantings. Severely infected lesions were discolored to red-purplish. Based on the morphological characteristics and analysis of rDNA, the fungus associated with the symptoms was identified as Erysiphe abeliicola U. Braun & S. Takam. This work provides the morphological feature of its anamorph for the first time, which is characterized by having multi-lobed hyphal appressoria and short foot-cells of conidiophores. Morphological characteristics of mature chasmothecia were consistent with the previous Japanese record of this species. The sequence of internal transcribed spacer region of ribosomal DNA obtained from a Korean sample showed that this species places in the section Microsphaera of the genus Erysiphe in phylogenetic position, corresponding with the classical taxonomy. This is the first report of E. abeliicola and its host plant in Korea. The host plant A.
grandiflora is newly listed in the host range of E. abeliicola.
Stem Rot of Common Bean (Phaseolus vulgaris L.) Caused by Sclerotium rolfsii
Kwon, Jin-Hyeuk ; Kang, Dong-Wan ; Kwak, Youn-Sig ; Choi, Ok-Hee ; Han, Seong-Sook ;
Research in Plant Disease, volume 18, issue 2, 2012, Pages 139~141
DOI : 10.5423/RPD.2012.18.2.139
Stem rot symptoms of common bean occurred sporadically in Jinju, Korea. The typical symptom included water-soaking on the main stem, rotting, wilting, and blighting, which eventually leads to death of the plant. The sclerotia, white to brown, spherical with size of 1-3 mm, formed over lesions and surface soil line. The optimum temperature for mycelial growth and sclerotia formation was
on potato dextrose agar (PDA). The typical clamp connections were observed in the hyphae of the fungus grown on PDA. Mycological characteristics and pathogenicity to host plants indicated that the fungus was Sclerotium rolfsii Saccardo. This is the first report of stem rot on common bean caused by S. rolfsii in Korea.