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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Research in Plant Disease
Journal Basic Information
Journal DOI :
Korean Society of Plant Pathology
Editor in Chief :
Volume & Issues
Volume 19, Issue 4 - Dec 2013
Volume 19, Issue 3 - Sep 2013
Volume 19, Issue 2 - Jun 2013
Volume 19, Issue 1 - Mar 2013
Selecting the target year
Optimal Spray Time, Interval and Number of Preventive Fungicides for the Control of Fruit Rots of Green and Gold Kiwifruit Cultivars
Kim, Gyoung Hee ; Lee, Young Sun ; Jung, Jae Sung ; Hur, Jae-Seoun ; Koh, Young Jin ;
Research in Plant Disease, volume 19, issue 1, 2013, Pages 1~6
DOI : 10.5423/RPD.2013.19.1.001
Optimal spray time, interval and number of preventive fungicides against fruit rots of kiwifruit were investigated at the orchard which both green kiwifruit cultivar 'Hayward' and gold kiwifruit cultivar 'Hort16A' are cultivating side by side during 2009 and 2010 growing seasons in Jeju island, Korea. The highest control efficiency was obtained from benomyl WP and followed by thiophanate-methyl WP and carbendazim+diethofencarb WP. The control efficacies of the fungicides were much higher when applied onto the kiwifruit canopy after the flowering time than before the flowering time but thereafter their control efficiencies were decreased drastically according to delays of spray timing. With increasing spray numbers of the fungicides, the control efficacy increased. However, optimal spray time, interval and number of the preventive fungicides for the effective control of fruit rots of kiwifruit were determined as 4 time-spray schedule with 2-week-interval just after the flowering time on both 'Hayward' and 'Hort16A' cultivars.
Changes in Sensitivity Levels of Botrytis spp. Population Isolated from Lily to Fungicides and Control under Field Condition
Hahm, Soosang ; Kyeong, Kicheon ; Kim, Byungryun ; Han, Kwangseop ; Choi, Jongjin ; Nam, Yunkyu ; Yu, Seunghun ;
Research in Plant Disease, volume 19, issue 1, 2013, Pages 7~11
DOI : 10.5423/RPD.2013.19.1.007
Forty eight isolates of Botrytis elliptica and 23 isolates of B. cinerea from several locations in Korea were tested for resistance to fungicides used in the farmer's fields. Isolation frequency of B. elliptica having
(effective concentration of 50%) value
to benomyl and mancozeb appeared highly, suggesting that the two fungicides are not effective in controlling leaf blight of lily in the field. The isolates were tested for resistance to fungicides procymidone and iprodione which were most commonly used in the farmer's fields. The rates of
to procymidome and iprodione were 93.7% and 100%, respectively, and those of
to diethofencarb+carbendazim and fludioxonil were 98.0% and 93.8%, respectively. In the rain-protected cultivation, control of leaf blight of lily was the most effective when iprodine, diethofencarb+carbendazim, and fludioxonil were sprayed alternately four times during the growing season.
Biological Control of Sclerotinia sclerotiorum in Lettuce Using Antagonistic Bacteria
Chon, Bong-Goan ; Park, Suji ; Kim, Jin-Won ;
Research in Plant Disease, volume 19, issue 1, 2013, Pages 12~20
DOI : 10.5423/RPD.2013.19.1.012
To isolate antagonistic bacteria against sclerotinia rot of lettuce, caused by Sclerotinia sclerotiorum, soil samples were collected from the diseased greenhouse field in Namyangju city, Gyeong-gi province from 2007 to 2008. A total of 196 bacterial isolates were isolated using serial dilution method. In dual culture assay in vitro, 26 isolates showed more than 80% of inhibition rates of mycelial growth of S. sclerotiorum. Based on 16S rDNA sequence analysis, the 26 isolates were identified as Bacillus megaterium, B. cereus, B. subtilis, Arthrobacter nicotianae, A. ramosus, Pseudomonas filiscindens, Stenotrophomonas maltophilia, Brevibacterium frigoritolerans and Sphingobacterium faecium. The 26 isolates inhibited the mycelial growth of S. sclerotiorum up to 80% and the sclerotial germination 0-100%. In the greenhouse pot test of ten isolates conducted in summer, 2 isolates B. megaterium (DK6) and B. cereus (C210) showed control efficacy on sclerotia viability of S. sclerotiorum, 20% and 35%, respectively. In the greenhouse pot test in winter, the disease incidence of the control group was 80%, whereas those of 9 isolates among 26 were approximately 20%. From the result, the 9 isolates are expected as potentially antagonistic bacteria for biological control of sclerotinia rot of lettuce caused by S. sclerotiorum.
Biological Control of White Rot in Garlic Using Burkholderia pyrrocinia CAB08106-4
Han, Kwang Seop ; Kim, Buyng Ryun ; Kim, Jong Tae ; Hahm, Soo Sang ; Hong, Ki Heung ; Chung, Chang Kook ; Nam, Yun Gyu ; Yu, Seung Hun ; Choi, Jae Eul ;
Research in Plant Disease, volume 19, issue 1, 2013, Pages 21~24
DOI : 10.5423/RPD.2013.19.1.021
White rot caused by Sclerotium cepivorum was reported to be severe soil-born disease on garlic. Disease progress of white rot of garlic (Allium sativum L.) was investigated during the growing season of 2009 to 2011 at Taean and Seosan areas. The white rot disease on bulb began to occur from late April and peaked in late May. The antifungal bacteria, Burkholderia pyrrocinia CAB08106-4 was tested in field bioassay for suppression of white rot disease. As a result of the nucleotide sequence of the gene 16S rRNA, CAB008106-4 strain used in this study has been identified as B. pyrrocinia. B. pyrrocinia CAB080106-4 isolate suppressed the white rot with 69.6% control efficacy in field test. These results suggested that B. pyrrocinia CAB08106-4 isolate could be an effective biological control agent against white rot of garlic.
Resistance of Commercial Tomato Cultivars to Meloidogyne arenaria and M. incognita
Kim, Donggeun ; Ryu, Younghyun ; Park, Hyunro ; Huh, Changseok ; Bae, Changhwan ;
Research in Plant Disease, volume 19, issue 1, 2013, Pages 25~30
DOI : 10.5423/RPD.2013.19.1.025
Root-knot nematodes (Meloidogyne spp.) are among the main pathogens of greenhouse crops worldwide. Plant resistance is currently the method of choice for controlling these pests. To select resistant tomato against two common species of root-knot nematodes, M. incognita and M. arenaria, 36 commercial tomato (Lycopersicon esculentum Mill.) cultivars were screened. Seventeen tomato cultivars were resistant to both root-knot nematodes: six in cherry tomato, 'Tenten', 'Cadillac', 'Cutti', 'Sweet', 'Ppotto', 'Lycopin-9', eight in globe tomato, 'Lovely 240', 'Dotaerang Dia', 'Cupirang', 'Dotaerang Master', 'Super Dotaerang', 'Dotaerang Season', 'Miroku', 'Hoyong', and three in root stock, 'Special', 'Fighting', and 'Magnet'.
Studies on HG Type of Heterodera glycines in Korea
Kim, Donggeun ; Choi, Insoo ; Han, Wonyoung ; Ryu, Younghyun ; Kim, Myungsik ; Bae, Changhwan ;
Research in Plant Disease, volume 19, issue 1, 2013, Pages 31~35
DOI : 10.5423/RPD.2013.19.1.031
Thirteen soybean cyst nematode (SCN) (Heterodera glycines) populations collected in Korea were examined in their HG type by their reproductivity on 7 Plant Introduction indicators for the identification of HG type. Six HG types were identified, HG type 0, 2, 5, 2.5, 1.2.7, and 2.5.7. HG type 2.5 was the most frequent (4 samples, 30.8%), followed by HG type 2.5.7 (3 samples, 23.0%). About 76.9% of SCN populations were reproduced on PI 88788, followed by PI 209332 (61.5%), PI 548316 ('Cloud') (30.8%), and PI 548402 ('Peking') (7.7%). No population could reproduce on PI 90763, PI 437654, thus, they could be used for resistant source for developing SCN resistant soybean in Korea.
Detection of Carnation necrotic fleck virus and Carnation ringspot virus Using RT-PCR
Lee, Siwon ; Kang, Eun-Ha ; Heo, Noh-Yeol ; Kim, Sang-Mok ; Kim, Yu-Jeong ; Shin, Yong-Gil ;
Research in Plant Disease, volume 19, issue 1, 2013, Pages 36~44
DOI : 10.5423/RPD.2013.19.1.036
Carnation is considered to be one of the top three cutting flowers in the world, which is a main crop with 21 billion annual volume of manufacture. The four carnation items such as cuttings, seed, plant and unrooted cuttings are imported and exported. Viruses can be easily transmitted during vegetative propagation of carnation. Carnation necrotic fleck virus (CNFV) and Carnation ringspot virus (CRSV) are designated as Korea plant quarantine viruses and inspected. This study was aimed to develop specific primer sets for easy and rapid detection of CNFV and CRSV. Two RT-PCR primer sets were efficiently amplified 288 and 447 bp fragments for CNFV and 503 549 bp fragments for CRSV. Furthermore, developed nested primer sets make possible to high sensitive detection and verification. CNFV nested PCR primer sets all produced band of 147 bp and CRSV nested PCR primer sets did bands of 395 and 347 bp. In addition, plasmid inserted 6 sequences in amplicon were used as a positive control to improve inspection confidence. The successful application of PCR module newly developed in this study will be highly useful for detect of CNFV and CRSV for quarantine inspections.
Disease Control Efficacy of the Extract of Magnolia officinalis against Perilla and Zoysiagrass Rusts
Yoon, Mi-Young ; Choi, Yong Ho ; Kang, Mun Seong ; Lee, Jae Hong ; Han, Seong Sook ; Myoung, In Sik ; Han, Byoung Soo ; Choi, Gyung Ja ; Jang, Kyoung Soo ; Kim, Jin-Cheol ;
Research in Plant Disease, volume 19, issue 1, 2013, Pages 45~48
DOI : 10.5423/RPD.2013.19.1.045
Rusts are plant diseases caused by pathogenic fungi of the order Pucciniales. Rusts can affect a variety of plants. Development of new effective and environmentally benign agents against rusts is of great interest. In this study, we investigated the disease control efficacy of the emulsion concentrate (EC10) and wettable powder (WP20) type formulations of the extract of Magnolia officinalis (Magjia90; containing honokiol and magnolol at 90%) against rust diseases of perilla and zoysiagrass in fields. The treatment of EC10 and WP20 of Magjia90 showed control values of 47.9% to 69.6% and Magjia90-WP20 reduced more effectively the development of rust symptoms on perilla plants than Magjia90-EC10. Magjia90-WP20 also highly suppressed zoysiagrass rust with control values of 65.7% to 80.5%. On the other hand, no harmful effect of Magjia90-EC10 and Magjia90-WP20 was observed on the perilla and zoysiagrass plants tested. The results strongly indicate that the extract of M. officinalis (Magjia90) can be used as a natural fungicide for the control of rust diseases.
First Report of Sclerotinia White Rot Caused by Sclerotinia nivalis on Panax ginseng in Korea
Cho, Hye Sun ; Shin, Jeong-Sup ; Kim, Jae-Hyun ; Hong, Tae-Kyun ; Cho, Dae-Hui ; Kang, Je Yong ;
Research in Plant Disease, volume 19, issue 1, 2013, Pages 49~54
DOI : 10.5423/RPD.2013.19.1.049
Sclerotinia white rot disease was observed on 5 and 6-year-old ginseng (Panax ginseng) roots in Hongchun, Cheorwon, and Yanggu, Gangwon Province, Korea from 2006 to 2010. Symptoms included a brownish watery soft rot of the roots, and black sclerotia were often found on the rotten roots. The causal agent of the disease was identified as Sclerotinia nivalis based on cultural characteristics and sequence analyses of the internal transcribed spacer region of rDNA and
-tubulin gene with 100% sequence similarity. Pathogenicity tests were performed on 2-year-old ginseng roots with mycelium plugs without wounds. A watery soft rot of the roots and black sclerotia were observed 10 days after inoculation. These symptoms were identical to those observed on naturally infected roots. The same fungus was re-isolated from the lesions induced by artificial inoculation. This is the first report of sclerotinia white rot caused by S. nivalis on P. ginseng in Korea.
Occurrence of Target Spot on Rosemary Caused by Corynespora cassiicola in Korea
Lee, Wang-Hyu ; Han, Sang-Jun ; Choi, In-Young ;
Research in Plant Disease, volume 19, issue 1, 2013, Pages 55~59
DOI : 10.5423/RPD.2013.19.1.055
The purpose of this experiment was to investigate the development of new spot disease on the leaf and stem of rosemary (Rosmarinus officinalis) in commercial greenhouses at Jeonju and Namwon in Korea. Incidence of target spot on rosemary was higher at the end of the rainy season with high humidity. Those symptoms were black ring spots (3-5 mm in diameter) and withering on green leaves and stems. Conidiophores and conidia were formed on the infected tissue in moist chamber and conidia were shown as the cylindrical and oval types in chain, ranged from 55 to
in length, and 7 to
in width. Conidia with eight to ten pseudosepta were formed on the conidiapore. The optimum growth temperature of isolates was
on the PDA medium under the dark condition. In the pathogenesis test, the target spot and withering symptoms were appeared on the leaves and stems 3 days after inoculation showing similar symptoms compared to those of in nature. The same fungus was re-isolated from infected lesion, indicating that Corynespora cassiicola caused leaf target spot and twig blight on rosemary. The rDNA ITS nucleotide sequences of the pure cultured isolate from the diseased area on rosemary showed 100% similarity to the sequences of C. cassiicola available in the GenBank database (JQ595296, JQ595297, FJ852715 and AY238606). Therefore, we report that the target spot of leaves and stems in rosemary was caused by C. cassiicola.