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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Research in Plant Disease
Journal Basic Information
Journal DOI :
Korean Society of Plant Pathology
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Volume & Issues
Volume 19, Issue 4 - Dec 2013
Volume 19, Issue 3 - Sep 2013
Volume 19, Issue 2 - Jun 2013
Volume 19, Issue 1 - Mar 2013
Selecting the target year
The Complete Nucleotide Sequence of a Korean Isolate Bean yellow mosaic virus from Freesia sp. and Comparison to Other Potyviruses
Choi, Sun-Hee ; Yoon, Ju-Yeon ; Ryu, Ki-Hyun ; Choi, Seung-Kook ;
Research in Plant Disease, volume 19, issue 2, 2013, Pages 77~83
DOI : 10.5423/RPD.2013.19.2.077
Bean yellow mosaic virus (BYMV; genus Potyvirus, family Potyviridae) causes severe losses to various legume species and a number of non-legume species, particularly freesia plants. In a survey of virus diseases in Gyeonggi province, Korea, BYMV isolates were identified from many cultivated freesia species. Here, we determined the complete nucleotide sequences of a BYMV freesia isolate (BYMV-Fr; accession number FJ492961). BYMV-Fr genome consists of 9,545 nucleotides (nt) excluding the poly (A) tail and encodes 3,057 amino acid (aa), with an AUG start and UAG stop codon, containing one open reading frame typical of a potyvirus polyprotein. The polyprotein of BYMV-Fr was divided to ten proteins and the cleavage sites of each protein were determined. The coat protein (CP) and polyprotein of BYMV-Fr were compared at the aa level with those of the previously reported 4 BYMV isolates. BYMV-Fr shared 90.1 to 97.1 and 91.0 to 92.5% at the CP and polyprotein homology. Interestingly, BYMV-Fr showed identities of a lower level at the nt level of 5' noncoding region (61.4 to 67.6%) and at the aa level of P1 (71.4 to 72.8%), comparing with four BYMV isolates. Based on the aa sequence diversity of CP and polyprotein, phylogenetic analysis with the four BYMV isolates showed two distinct groups and BYMV-Fr and most BYMV isolates were most closely related to the clover yellow vein virus among 52 potyviruses. To our knowledge, this is the first report of the complete genome sequence of BYMV freesia strain.
Analysis of the Factors for Decrease of Rice Stripe Disease in Chungnam Province
Kim, Byung-Ryun ; Jeong, Tae-Woo ; Han, Kwang-Seop ; Hahm, Soo-Sang ; Kim, Young-Jin ; Nam, Yun-Gyu ; Choi, Hong-Soo ; Kim, Jeong-Soo ; Yu, Seung-Hun ;
Research in Plant Disease, volume 19, issue 2, 2013, Pages 84~89
DOI : 10.5423/RPD.2013.19.2.084
The incidence factors of Rice stripe virus (RSV) were analyzed by studying the population density and the viruliferous insect rate (VIR) of small brown planthopper (SBPH), the incidence of stripe disease, alternate host, and susceptible cultivar in Chungnam Province. The population of overwintering SBPH had been decreasing, but the VIR of overwintering SBPH had not been differing for three years, 2008 to 2010. No RSV was detected in the natural host plants, such as short awn, annual bluegrass, and barley. In 2009, relatively large population of SBPH with the VIR of 5.4% migrated from China. However, there was no evidence relating of migration large amount of SBPH from China in 2008 and 2010. Also the infection rate of RSV in rice was less than 1% in these periods. The cultivation area of the susceptible varieties had steadily decreased from 41% to 19% from 2007 to 2009. Therefore, the reduction factors of rice stripe disease in Chungnam Province with higher influx of inoculum could be with an appropriate forecasting and chemical control, cultivation of resistant varieties, changes in the cropping system, and the low winter-spring temperature.
Superficial Tuber Necrosis in Potato Cultivar 'Haryeong' Caused by Potato virus Y
Lee, Young-Gyu ; Kim, Jeom-Soon ; Kim, Ju-Il ; Park, Young-Eun ;
Research in Plant Disease, volume 19, issue 2, 2013, Pages 90~94
DOI : 10.5423/RPD.2013.19.2.090
Potato cv. 'Haryeong' was bred with high solids, resistance to late blight and good culinary quality. It has been registered as new potato variety in 2005. Tuber necrosis symptoms such as severe superficial necrosis, raised surface lesions and ringed necrotic areas were found in tubers of 'Haryeong' during storage of seed potatoes in 2010. Potato virus Y (PVY) was detected from these symptomatic tubers by the analysis of RT-PCR using a primer set specific to coat protein gene of PVY. The nucleotide sequence of RT-PCR product (
) was determined and compared to those of other strains, such as
registered in GeneBank. The result showed that
was exactly the same as
, Korean isolate reported in 2005, except two nucleotides. To verify the PVY was responsible for the tuber necrosis symptoms shown in the tubers of 'Haryeong', a bioassay was done using two viruses (PVY and Potato leafroll virus) and five potato cultivars ('Haryeong', 'Superior', 'Atlantic', 'Dejima', and 'Chubaek'). As expected, the same necrosis symptom appeared in tubers of 'Haryeong' infected with PVY only during the storage period.
Development of Efficient Screening Method for Resistance of Cabbage Cultivars to Black Rot Disease Caused by Xanthomonas campestris pv. campestris
Lee, Ji Hyun ; Kim, Jin-Cheol ; Jang, Kyoung Soo ; Choi, Yong Ho ; Ahn, Kyoung Gu ; Choi, Gyung Ja ;
Research in Plant Disease, volume 19, issue 2, 2013, Pages 95~101
DOI : 10.5423/RPD.2013.19.2.095
Black rot caused by Xanthomonas campestris pv. campestris (Xcc) is one of the most serious diseases of crucifers world-wide. To establish the efficient screening method for resistant cabbage to Xcc, different inoculation methods, inoculation positions, growth stages of seedlings, and incubation temperatures after inoculation were investigated with the seven cabbage cultivars showing different resistance degrees to the pathogen. Clipping with mouse-tooth forceps was better inoculation method than piercing with 18 pins or cutting with scissors to distinguish the level of resistance and susceptibility. In inoculation using mouth-tooth forceps, clipping the edges of the leaves near veins is more effective than injuring the veins of the leaves directly. In addition, the inoculated plants kept at
showed more clear resistant and susceptible responses than those kept at 26 or
. On the basis of the results, we suggest that an efficient screening method for resistance of cabbage cultivars to black rot is to clip the edges of the leaves near veins of the four-week-old seedlings with mouth-tooth forceps dipped in a suspension of Xcc at a concentration of
cfu/ml and incubate the inoculated plants in a growth room at
with 12-hr light a day.
Aspect of Incidence of the Major Citrus Diseases Recently
Hyun, Jae-Wook ; Yi, Pyoung-Ho ; Hwang, Rok-Yeon ; Moon, Kyung-Hwan ;
Research in Plant Disease, volume 19, issue 2, 2013, Pages 102~107
DOI : 10.5423/RPD.2013.19.2.102
The percent of diseased fruit by Diaporthe citri was the most 16.8% in 2010 and the least 3.8% in 2005, and the average was 8.8% from 2003 to 2012. The degrees of disease incidence were relatively high, 17.0, 22.6, 19.2 and 18.9%, in 2003, 2004, 2007 and 2010, respectively. The percent of diseased fruit by Elsinoe fawcettii was the most 0.72% in 2004, and then gradually decreased to 0.08% in 2008, and the average was from 0.28% from 2004 to 2011. In case of canker by Xanthomonas citri subsp. citri, the percent of diseased fruit was the most 2.19% in 2004, and gradually decreased to 0.08 and 0.10% in 2009 and 2010, respectively. The average was 0.81% from 2004 to 2012. The melanose by D. citri was most in south-east area of Jeju Island, and east and west in case of canker. The melanose symptoms were initially appeared from mid June, mainly peaked late of July to late August. The canker symptoms were initially produced on leaves of natsudaidai and grapefruit cultivated in open field middle of June in 2010 and 2011 and late of May in 2012. The scab disease was firstly appeared approx. early or mid May and then rapidly increased late of May to middle of June. The inoculum was mainly produced from dead twigs late June to early of August.
Reduction of Bacterial Wilt Diseases with Eggplant Rootstock EG203-Grafted Tomatoes in the Field Trials
Lee, Mun Haeng ; Kim, Ji Kwang ; Lee, Hee Kyoung ; Kim, Keyng Jae ; Yu, Seung Hun ; Kim, Young Shik ; Lee, Youn Su ;
Research in Plant Disease, volume 19, issue 2, 2013, Pages 108~113
DOI : 10.5423/RPD.2013.19.2.108
Wilt damage on tomato plants caused by Ralstonia solanacearum has been increased as the areas of tomato cultivation increased during the warm seasons. Also, the tomato rootstocks used to prevent the disease occurrence are not effective in the highly prevailing regions. Therefore, bacterial wilt resistant eggplant rootstock EG203, collected from AVRDC, was tested for its effect to deter the Ralstonia solanacearum wilt disease in the greenhouses at Buyeo Tomato Experiment Station from 2003 to 2005, and at Gumi, Kyungpook province from 2009-2011. Planting of eggplant rootstock EG203 should be done three weeks before the planting of tomato scions so that they can have similar stem diameter (2.5-3.0 mm) and can be easily grafted. Both insertion and inarching grafting showed 93-96% success rates. In the greenhouse tests at Buyeo Tomato Experiment Station from 2003 to 2005, eggplant rootstock EG203-grafted tomatoes showed the disease occurrence of 4.3%. On the other hand, non-grafted or other commercial rootstock-grafted tomatoes showed disease occurrence of 58.0% and 25.0-36.7%, respectively. In the greenhouse tests at Gumi, Kyungpook province in 2009, the disease occurrence on the EG203-grafted and non-grafted tomatoes was 2-5% and 20-80%, respectively. In 2010, at Gumi, Kyungpook province, when the wilt disease occurred slightly, the tomatoes grafted with tomato rootstocks B-blocking and Chung-gang, and eggplant rootstock EG203 showed similar disease severities, but EG203-grafted tomatoes formed lately cluster, resulting in the reduction of yield compared to tomato-grafted tomatoes. In 2011, at Gumi, Kyungpook province, when the wilt disease occurred severely, the tomato rootstocks 'B-blocking' and Chung-gang and eggplant rootstock EG203-grafted tomatoes showed disease occurrences of 60-85% and 0-1%, respectively. Therefore, it was concluded that tomato rootstocks 'B-blocking' and 'Chung-gang' are more useful in the areas contaminated with low levels of pathogen and eggplant rootstock EG203 is more useful in the areas contaminated with high levels of pathogen.
Seed Transmission Rates of Bean pod mottle virus and Soybean mosaic virus in Soybean May Be Affected by Mixed Infection or Expression of the Kunitz Trypsin Inhibitor
Nam, Moon ; Bae, Hanhong ; Hammond, John ; Domier, Leslie L. ; Youn, Young-Nam ; Lee, Bong-Choon ; Lim, Hyoun-Sub ;
Research in Plant Disease, volume 19, issue 2, 2013, Pages 114~117
DOI : 10.5423/RPD.2013.19.2.114
To facilitate their spread, plant viruses have developed several methods for dispersal including insect and seed transmission. While insect transmission requires virus stability against insect digestion, seed-transmitted viruses have to overcome barriers to entry into embryos. Bean pod mottle virus (BPMV) is transmitted through seed at levels typically below 0.1%, but co-infection with Soybean mosaic virus (SMV) enhanced the seed transmission rate of BPMV in one experiment. In contrast, the rate of SMV seed transmission was not affected by BPMV co-infection. In a second preliminary study, the rate of SMV transmission was lower in an isoline of Williams 82 that contained a null mutation for the Kunitz trypsin inhibitor gene than in Williams 82. In this preliminary study, we observed that factors such as protease inhibitor expression and dual infection may affect the frequency of seed transmission of BPMV and SMV.
Stem Rot of Sweet Potato (Ipomoea batatas) Caused by Sclerotium rolfsii in Korea
Kim, Ju-Hee ; Kim, Shin-Chul ; Cheong, Seong-Soo ; Choi, Kyu-Hwan ; Kim, Du-Yeon ; Shim, Hong-Sik ; Lee, Wang Hyu ;
Research in Plant Disease, volume 19, issue 2, 2013, Pages 118~120
DOI : 10.5423/RPD.2013.19.2.118
During the early spring of 2007 to 2009, stem rot of sweet potato (Ipomoea batatas L.) caused by Sclerotium rolfsii occurred in seedling stage grown in plastic film house at Iksan. In seedling stage of sweet potato, symptoms were initially appeared in yellowing and then the seedlings were eventually wilted. The fungus produced abundant white silky mycelium on infected tissues and soil line. Seedlings were very susceptible and died quickly once they were infected. The whole area of a petridish was rapidly covered with white mycelium on agar medium. Sclerotia began to produce after 7 days of mycelial growth and white sclerotia quickly melanized to a dark brown coloration. The causal agent isolated from the diseased plants was identified as Sclerotium rolfsii Saccardo on the basis of the morphological and cultural characteristics. All isolates of S. rolfsii caused similar symptoms on the host petioles by artificial inoculation.
Pathogenicity and Host Range of Pathogen Causing Black Raspberry (Rubus coreanus) Anthracnose in Korea
Jeong, Uh Seong ; Kim, Ju Hee ; Lee, Ki Kwon ; Cheong, Seong Soo ; Lee, Wang Hyu ;
Research in Plant Disease, volume 19, issue 2, 2013, Pages 121~123
DOI : 10.5423/RPD.2013.19.2.121
The strains of Colletotrichum gloeosporioides, C. coccodes, C. acutatum isolated from black raspberry were pathogenic to apple and strawberry after dropping inoculation, but showed weak pathogenicity in hot-pepper and tomato. The anthracnose pathogens of C. gloeosporioides, C. orbiculare, C. acutatum isolated from apple, hot-pepper and pumpkin showed pathogenicity in black raspberry. Moreover, the anthracnose pathogens isolated from apple caused disease symptoms in non-wounded inoculation.
A Pathotype of Pepper mild mottle virus Causing Necrotic Spot Symptoms in Paprika Fruit
Choi, Gug-Seoun ; Choi, Sung-Kook ; Cho, Jeom-Deog ; Cho, In-Sook ;
Research in Plant Disease, volume 19, issue 2, 2013, Pages 124~127
DOI : 10.5423/RPD.2013.19.2.124
Black necrotic spots were observed from the fruits of paprika that were cultivating in a vinylhouse. The casual agents of the symptom were identified as several isolates of Pepper mild mottle virus (PMMoV) by responses of indicator plants, electron microscopy, and RT-PCR analysis. Symptoms of the viral disease were mild mottle in the young leaves, necrotic spots on the fruits and the fruit apex of paprika, but the symptoms were not shown on the mature leaves. All of the PMMoV isolates were determined as
pathotypes from the biological responses on the chilli pepper lines used for discrimination of tobamovirus pathotypes. Pathogenicity of the PMMoV isolates was also confirmed using mechanical inoculation method to paprika seedlings. The coat protein (CP) genes of the PMMoV isolates were compared at the nucleotide and amino acid levels with the previously published PMMoV isolate. The isolates share 96 to 99% CP nucleotide identity among the isolates. The CP of
-pathotype PMMoV-P2 presented Met at position 139, But the CPs of
-pathotype PMMoVs from paprika showed Met to Asn substitution at the same position. This is the first report of identification of
-pathotype PMMoV isolates from paprika in Korea.