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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Research in Plant Disease
Journal Basic Information
Journal DOI :
Korean Society of Plant Pathology
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Volume & Issues
Volume 19, Issue 4 - Dec 2013
Volume 19, Issue 3 - Sep 2013
Volume 19, Issue 2 - Jun 2013
Volume 19, Issue 1 - Mar 2013
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Inhibitory Effects of Resveratrol and Piceid against Pathogens of Rice Plant, and Disease Resistance Assay of Transgenic Rice Plant Transformed with Stilbene Synthase Gene
Yu, Sang-Mi ; Lee, Ha Kyung ; Jeong, Ui-Seon ; Baek, So Hyeon ; Noh, Tae-Hwan ; Kwon, Soon Jong ; Lee, Yong Hoon ;
Research in Plant Disease, volume 19, issue 3, 2013, Pages 177~182
DOI : 10.5423/RPD.2013.19.3.177
Resvestrol has been known to inhibit bacterial and fungal growth in vitro, and can be accumulated in plant to concentrations necessary to inhibit microbial pathogens. Hence, stilbene synthase gene has been used to transform to synthesize resveratrol in heterologous plant species to enhance resistance against pathogens. In the present study, we investigated the antimicrobial activities of resveratrol and piceid to bacterial and fungal pathogens, which causing severe damages to rice plants. In addition, disease resistance was compared between transgenic rice varieties, Iksan 515 and Iksan 526 transformed with stlibene synthase gene and non-transgenic rice varieties, Dongjin and Nampyeong. Minimum inhibitory concentration of resveratrol for Burkolderia glumae was 437.5
, and the mycelial growth of Biplaris oryzae was slightly inhibited at concentration of 10
. However, other bacterial and fungal pathogens are not inhibited by resveratrol and piceid. The expression of the stilbene synthase gene in Iksan 515 and Iksan 526 did not significantly enhanced resistance against bacterial grain rot, bacterial leaf blight, sheath blight, and leaf blight. This study is the first report on the effect of resveratrol and piceid against pathogens of rice plant, and changes of disease resistance of transgenic rice plants transformed with stilbene synthase gene.
Fungal Distribution and Varieties Resistance to Kernel Discoloration in Korean Two-rowed Barley
Shin, Sang-Hyun ; Seo, Eun-Jo ; Choi, Jae-Seong ; Kang, Chun-Sik ; Lee, JungKwan ; Park, Jong-Chul ;
Research in Plant Disease, volume 19, issue 3, 2013, Pages 183~187
DOI : 10.5423/RPD.2013.19.3.183
Barley kernel discoloration (KD) leads to substantial loss in value through downgrading and discounting of malting barley. The objective of this research is to investigate fungal distribution and varieties resistance to KD in Korean two-rowed barley. Several fungal organisms including Alternaria spp., Fusarium spp., Aspergillus spp., Epicoccum spp. and Rhizopus spp. were isolated from Korean two-rowed barley representing KD. The symptoms of KD were brown and black discolorations of the lemma and palea. The most frequently detected fungal species was Alternaria spp. which exhibited 69.1% and 72.2% in 2011 and 2012, respectively. Epicoccum spp., Fusarium spp., and Aspergillus spp. were also detected. Fusarium spp., primary pathogen of barley head blight, were rarely occurred in the 2011 and their occurrence increased to 4.7% in 2012. Twenty cultivars of Korean two-rowed barely were evaluated to KD. The average percentage of KD was 8.0-36.0% in 2011 and 5.2-36.6% in 2012. Two cultivars ('Sacheon 6' and 'Dajinbori') showed KD of 6.2% to 8.8% and determined resistant, however 'Samdobori' and 'Daeyeongbori' demonstrating KD of 22.2-36.6% were highly susceptible. 'Jinyangbori', 'Danwonbori', 'Sinhobori' and 'Kwangmaegbori' showing KD of less than 15% were moderately resistant cultivar.
Screening for Resistance to Downy Mildew among Major Commercial Cucumber Varieties
Lee, Jung-Sup ; Han, Kyung-Sook ; Lee, Seong-Chan ; Soh, Jae-Woo ;
Research in Plant Disease, volume 19, issue 3, 2013, Pages 188~195
DOI : 10.5423/RPD.2013.19.3.188
This study was carried out for the downy mildew resistant test between 2010 and 2012. A set of 22 accessions belonging to 2 wild species and 20 varieties of the genus Cucumis, originating mainly from the Asian Vegetable Research and Development Center (AVRDC) Gene Centre, was evaluated for resistance to Pseudoperonospora cubensis, causal agent of cucumber downy mildew. The youngest fully expanded true leaves were found suitable for in vitro screening. Both leaf discs and full leaves could be kept fresh longer when applying 0.2
of gibberellin acid (GA). The incubation temperature of
was found to be the most suitable temperature for symptom development comparing with 15 and
. Symptom development was faster when contact diseased leaf discs (2 weeks after inoculation) on to fresh leaf samples comparing with using conidia suspension (
spores/ml). The numbers of spots in 'C-19' were lower than other varieties. 'C-19' variety was also showed the highest level of downy mildew resistant at
chamber in 6 days after inoculating with pathogen and displayed 0.90 (under 10%) of the infected rate. However, other varieties displayed susceptible in the pathogen sprayed plots. 'C-19' was the most resistant variety and no lesion was observed. Based on all data, 'C-19' can be a useful variety for the prevention of downy mildew.
Establishment of Economic Threshold by Evaluation of Yield Component and Yield Damages Caused by Leaf Spot Disease of Soybean
Shim, Hongsik ; Lee, Jong-Hyeong ; Lee, Yong-Hwan ; Myung, Inn-Shik ; Choi, Hyo-Won ;
Research in Plant Disease, volume 19, issue 3, 2013, Pages 196~200
DOI : 10.5423/RPD.2013.19.3.196
This study was carried out to investigate yield loss due to soybean leaf spot disease caused by Cercospora sojina Hara and to determine the economic threshold level. The investigations revealed highly significant correlations between disease severity (diseased leaf area) and yield components (pod number per plant, total grain number per plant, total grain weight per plant, percent of ripened grain, weight of hundred seed, and yield). The correlation coefficients between leaf spot severity and each component were -0.90, -0.90, -0.92, -0.99, -0.90 and -0.94, respectively. The yield was inversely proportional to the diseased leaf area increased. The regression equation, yield prediction model, between disease severity (x) and yield (y) was obtained as y = -3.7213x + 354.99 (
= 0.9047). Based on the yield prediction model, economic injury level and economic threshold level could be set as 3.3% and 2.6% of diseased leaf area of soybean.
Contamination of Chinese Cabbage Soil with Plasmodiophora brassicae
Soh, Jae-Woo ; Han, Kyung-Sook ; Lee, Seong-Chan ; Lee, Jung-Sup ;
Research in Plant Disease, volume 19, issue 3, 2013, Pages 201~207
DOI : 10.5423/RPD.2013.19.3.201
This research was performed to establish basic technology for Chinese cabbage clubroot chemical control by investigating the soil contamination of Plasmodiophora brassicae in major producing regions of fall Chinese cabbage. PCR primers were developed to detect P. brassicae, a causal agent of Chinese cabbage club-root that generally occurs in Cruciferae family. A primer set, PbbtgF761 and PbbtgR961, specifically amplified a 245 bp fragment from P. brassicae only. At places well known for fall Chinese cabbage, 10 out of 33 in Haenam-gun, 5 out of 13 in Yeongam-gun and Yeonggwang-gun, 1 out of 6 in Gochang-gun, 2 out of 12 in Hongseong-gun, and 5 out of 17 in Dangjin-si resulted positive for P. brassicae contamination. The results show that the soil contamination rate of P. brassicae was 30.3% in Haenam-gun, 38.5% in Yeongam-gun and Yeonggwang-gun, 16.7% in Gochang-gun, 16.7% in Hongseong-gun, and 29.4% in Dangjin-si. The six places where Chinese cabbage clubroot was visible by naked eye were 100% confirmed by the PCR test of the P. brassicae contaminated soil. Thus, simple PCR test may be utilized as an index to decide on chemical control of P. brassicae.
Control Effects of 3-(4-Hydroxyphenyl)-propionic Acid Isolated Xenorhabdus nematophila K1 against Phytophthora Blight and Anthracnose of Red Pepper
Cheon, Wonsu ; Kim, Doyeon ; Kim, Yonggyun ; Hong, Yong Pyo ; Yi, Youngkeun ;
Research in Plant Disease, volume 19, issue 3, 2013, Pages 208~215
DOI : 10.5423/RPD.2013.19.3.208
3-(4-Hydroxyphenyl)-propionic acid (HPP) is a bacterial metabolite synthesized and released by an entomopathogenic bacterium Xenorhabdus nematophila K1. In this study, the control efficacy of HPP was tested against Phytophthora blight and anthracnose of red pepper plants. HPP suppressed mycelial growth of Phytophthora blight and anthracnose pathogens. Under natural sunlight condition, HPP maintained the antifungal activity on the diseases for at least twenty five days. The antifungal activity was not decreased even in the condition of soil-water. It was proved that HPP was able to penetrate the roots and travel upward of the red pepper plants. When HPP suspension was applied to soil rhizosphere before transplanting the red pepper seedlings or was regularly sprayed to the foliage of the plants with ten days interval, it resulted in significant reduction of the disease occurrences (Phytophthora blight and anthracnose) without any phytotoxicity. These results suggested that HPP can be developed to a systemic agrochemical against Phytophthora blight and anthracnose of red pepper plants.
Resistance of Soybean Cultivars to Heterodera glycines HG type 2.5 in Korea
Kim, Donggeun ; Choi, Insoo ; Ryu, Younghyun ; Lee, Younsu ;
Research in Plant Disease, volume 19, issue 3, 2013, Pages 216~219
DOI : 10.5423/RPD.2013.19.3.216
A total of 75 soybean cultivars developed in Korea was screened against soybean cyst nematode (SCN), Heterodera glycines HG type 2.5. Cysts developed on soybean cultivars ranged from 104 to 624 cysts per pot. There was no resistant cultivar but 'Jangyeopkong', 'Saealkong', 'Miryangkong', and 'Mansukong' were moderately resistant; 33 cultivars were moderately susceptible and the other cultivars were susceptible. 'Jangyeopkong', 'Saealkong', 'Miryangkong', and 'Mansukong' could be recommended for soybean fields infested with SCN until developing SCN resistant soybean.
Development of RT-PCR and Nested PCR for Detecting Four Quarantine Plant Viruses Belonging to Nepovirus
Lee, Siwon ; Kang, Eun-Ha ; Shin, Yong-Gil ; Lee, Su-Heon ;
Research in Plant Disease, volume 19, issue 3, 2013, Pages 220~225
DOI : 10.5423/RPD.2013.19.3.220
For quarantine purpose, we developed the RT- and nested PCR module of Tomato black ring virus (TBRV), Arabis mosaic virus (ArMV), Cherry leafroll virus (CLRV) and Grapevine fanleaf virus (GFLV). The PCR modules, developed in this study make diagnosis more convenient and speedy because of same PCR condition. And also, the methods are more accurate because it can check whether the result is contamination or not using the mutation-positive control. We discard or return the 27 cases of Nepovirus infection seed by employing the module past 3 years. This study provides a rapid and useful method for detection of four quarantine plant viruses.
Pink Mold Rot on Unishiu Orange (Citrus unshiu Mac.) Caused by Trichothecium roseum (Pers.) Link ex Gray in Korea
Kwon, Jin-Hyeuk ; Kang, Dong-Wan ; Choi, Okhee ; Shim, Hong-Sik ;
Research in Plant Disease, volume 19, issue 3, 2013, Pages 226~228
DOI : 10.5423/RPD.2013.19.3.226
In 2012, a pink mold rot was observed on unishiu orange (Citrus unshiu Mac.) fruits at the Wholesale Market for Agricultural Products, Jinju, Korea. The symptom on unishiu orange was a water-soaked lesion on the surface of fruit, which later on enlarged to form softened brown rot lesions. The diseased fruits were covered with pink-colored mold, consisting of conidia and conidiophores of the pathogen. Optimum temperature for mycelial growth was
. Conidia were hyaline, smooth, 2-celled, and thick-walled conidia with truncate bases, ellipsoidal to pyriform, characteristically held together zig-zag chains and
in size. Conidiophore was erect, colorless, unbranched, and 4-5
wide. On the basis of mycological characteristics, pathogenicity test, and molecular analysis with complete ITS rDNA region, the causal fungus was identified as Trichothecium roseum (Pers.) Link ex Gray. This is the first report of pink mold rot caused by T. roseum on unishiu orange in Korea.
Occurrence of Sclerotium Rot of Cucumber Caused by Sclerotium rolfsii
Kwon, Jin-Hyeuk ; Lee, Sang-Dae ; Choi, Okryun ; Shen, Shun-Shan ; Shim, Hong-Sik ;
Research in Plant Disease, volume 19, issue 3, 2013, Pages 229~232
DOI : 10.5423/RPD.2013.19.3.229
Sclerotium rot of cucumber (Cucumis sativus L.) occurred at the experimental field of Gyeongsangnam-do Agricultural Research and Extension Services in July 2012. The typical symptoms included wilt, rot, and water-soaking on stems and fruits and severely infected plants eventually died. White mycelial mats spread over lesions, and then sclerotia were formed on fruit and near soil line. The sclerotia were globoid in shape, white to brown in color and 1-3 mm in size and the hyphal width was 4-8
. The optimum temperature for mycelial growth and sclerotia formation on PDA was
. The typical clamp connections were observed in the hyphae of the fungus grown on PDA. For further identification, the complete internal transcribed spacer (ITS) rDNA region was amplified and sequenced. On the basis of mycological characteristics, ITS rDNA region comparison, and pathogenicity to host plants, this fungus was identified as Sclerotium rolfsii Saccardo. This is the first report of sclerotium rot on cucumber caused by S. rolfsii in Korea.
Incidence of Viral Infection of Main Garlic Growing Areas of Gangwon Province in Korea
Shin, Ji-Eun ; Lee, Yeong-Gyu ; Kim, Byung-Sup ;
Research in Plant Disease, volume 19, issue 3, 2013, Pages 233~236
DOI : 10.5423/RPD.2013.19.3.233
This research was conducted to investigate the incidence of viral infection in Samcheok-shi and Jeongseon-gun, main garlic growing areas of Gangwon province in Korea. Four primers sets (GLV, LYSV, GCLV, OYDV) were used for RT-PCR to test of 100 samples collected from each location. Infection rates of GLV, LYSV, GCLV and OYDV of garlic samples from Samcheok-shi and Jeongseon-gun were 95, 95, 92 and 33%, respectively. All garlic samples tested in this research were infected at least one virus. Coinfection of 3 kinds of virus (GLV, LYSV and GCLV) and 4 kinds of virus (GLV, LYSV, GCLV and OYDV) were 60 and 25%, respectively. While infections of GLV, LYSV and GCLV were evenly found in both two regions, OYDV infection of garlic samples collected from Jeongseon-gun was higher than Samcheok-shi. Viral infections at garlic fields of whole country and Gangwon province are very serious now. Therefore, it is very necessary to develop technic for virus-free bulb and renew seed bulb of garlic.