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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Research in Plant Disease
Journal Basic Information
Journal DOI :
Korean Society of Plant Pathology
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Volume & Issues
Volume 20, Issue 4 - Dec 2014
Volume 20, Issue 3 - Sep 2014
Volume 20, Issue 2 - Jun 2014
Volume 20, Issue 1 - Mar 2014
Selecting the target year
Effect of Gamma Irradiation and Its Convergent Treatments on Lily Leaf Blight Pathogen, Botrytis elliptica, and the Disease Development
Kim, Ji-Hoon ; Yun, Sung-Chul ;
Research in Plant Disease, volume 20, issue 2, 2014, Pages 71~78
DOI : 10.5423/RPD.2014.20.2.071
Gamma irradiation and its convergence with nano-silver particles and sodium dichloroisocyanurate (NaDCC) were investigated to inhibit germination and mycelial growth of Botrytis elliptica, the pathogen of lily leaf blight. In addition, the same treatments were studied on the process of disease development with detached leaf of lily cv. Siberia. Spray inoculation, which is closer to natural infection than wound inoculation, can be a way to investigate infection ability of the treated pathogen. The irradiating dose required to reduce the population by 90%,
, was 526 Gy irradiating with 0-2000 Gy gamma ray on the conidial suspension as well as the growing mycelia. Even at 2000 Gy, the mycelium was not killed but just delayed its growth at 1-2 days behind. Convergent treatment with 40 mg/l of NaDCC just before 200 Gy gamma irradiation was the best way to decrease the conidial germination about 1/1000 times. The control values of gamma irradiation were 23% and 19.5% at wound inoculation and spray inoculation, respectively. On wound-inoculation, the control value of NaDCC only was 89%, and that of NaDCC convergent with 200 Gy gamma irradiation was 32%. On sprayinoculation, the highest control value was NaDCC at 50%, and that of NaDCC convergent with gamma irradiation was 24%.
Application of Gamma Irradiation and Its Convergent Treatments on Several Varieties of Oriental Hybrid Lily to Control Leaf Blight
Kim, Ji-Hoon ; Koo, Tae-Hoon ; Hong, Sung-Jun ; Yun, Sung-Chul ;
Research in Plant Disease, volume 20, issue 2, 2014, Pages 79~86
DOI : 10.5423/RPD.2014.20.2.079
In order to seek more eco-friend, economic and safer quarantine method than current methyl bromide fumigation, the convergent treatment with 200 Gy of gamma irradiation and several chemicals such as nano-siver particles (NSS), sodium dichloroisocyanurate (NaDCC) was tried on the cuttings of lily in the packing of catonnage box for export. With 6 independent experiments of gamma irradiation on the three lily cultivars, cvs. Siberia, Le reve and Sorbonne, incidence and severity of lily leaf blight was investigated on leaves and petals at 8-d after infection. 200 Gy of gamma irradiation decreased at 13-25% of severity on the leaf of Sorbonne, but it increased at 2-5% of severity on the leaf of Siberia and Le reve. Chemical substitutes such as NSS and NaDCC were not effective to control of lily blight on cuttings. By 200 Gy of gamma irradiation treatment, chlorophyll contents were statistically significantly decreased at 12-d after irradiation and the longevities vaselife of fully open flower of Siberia and Sorbonne were increased at 0.4 to 1.2 days. In addition, the relative fresh weights of the gamma irradiated cuttings were severely dried compared to the non-irradiated control. On the other hands, the symptoms of phyto-toxicity of high dose gamma irradiation at 1 or 2 kGy on cv. Siberia were to be blight at the tip of bloom, bent necks of flower, and delayed the process of flowering.
Environmental Factors on the Development of Root Rot on Ginseng Caused by Cylindrocarpon destructans
Lee, Jung Sup ; Han, Kyung Sook ; Lee, Seong Chan ; Soh, Jae Woo ; Kim, Doo Wook ;
Research in Plant Disease, volume 20, issue 2, 2014, Pages 87~94
DOI : 10.5423/RPD.2014.20.2.087
The fungus Cylindrocarpon destructans is the cause of root rot in many ginseng production areas in Korea. A total of 57 isolates of C. destructans were recovered from diseased roots in a survey of ginseng-growing fields from 2011-2012. Among these isolates, 37% were classified as highly virulent (causing lesions on unwounded mature roots) and 61% were weakly virulent(causing lesions only on previously wounded roots). Radial growth of highly and weakly virulent isolates on potato dextrose agar was highest at
and there was no growth at
. Mycelial mass production was significantly (P = 0.05) lower at pH 7.0 compared with pH 5.0. To study the effects of pH (5.0 and 7.0) and wounding on disease development, ginseng roots were grown hydroponically in nutrient solution. Lesions were significantly larger (P < 0.01) at pH 5.0 compared with pH 7.0 and wounding enhanced disease by a highly virulent isolate at both pHs. In artificially infested soil, 2-yearold ginseng roots were most susceptible to Cylindrocarpon root rot among all root ages tested (1 to 4 years) when evaluated using a combined scale of disease incidence and severity. Root rot severity was significantly (P<0.05) enhanced by increasing the inoculum density from
of soil to
Envrionment-Friendly Effects of Espil and Copper Hydroxide for Prevention of Powdery Mildew on Cucumber, Tomato, and Red Pepper
Soh, Jae-Woo ; Han, Kyung-Sook ; Lee, Seong-Chan ; Lee, Jung-Sup ; Park, Jong-Han ;
Research in Plant Disease, volume 20, issue 2, 2014, Pages 95~100
DOI : 10.5423/RPD.2014.20.2.095
This research was performed to examine the effects of mixed espil and copper hydroxide for powdery mildew prevention on cucumber, tomato and pepper. On prevention effect for powdery mildew on cucumber, results revealed that mixed espil and copper hydroxide with a ratio of 4 : 1, disease incidence rate was 18.9% and prevention effect was 69.9%. Another treatment with a ratio of 8 : 1 showed an disease incidence rate of 18.1% and prevention effect of 71.1%, thus, showed great effectiveness. For powdery mildew on tomato, espil and copper hydroxide were mixed using the ratio 4 : 1, results showed an disease incidence rate of 12.4% and prevention effect of 85.3%. Treatment using the ratio of 8 : 1, results showed an disease incidence rate of 14.3% and prevention effect of 83.0%, thus, showed great effectiveness. For powdery mildew on redpepper, espil and copper hydroxide were mixed using the ratio of 4 : 1 with results showed disease incidence rate of 17.7% and prevention effect of 83.0%. From the results, this treatment is the most effective with the lowest attack rate and highest prevention effect. Deducing from the study, it was found out that using mixed espil and copper hydroxide using the ratios 4 : 1 or 8 : 1 are the most effective method for powdery mildew prevention. Mixed ratio of 4 : 1 or 8 : 1 was most effective for preventing powdery mildew on cucumber and tomato, while the espil and copper hydroxide ratio of 4 : 1 was the most effective method for powdery mildew prevention on pepper.
Suppression of Citrus Canker by Pretreatment with Rhizobacterial Strains Showing Antibacterial Activity
Yang, Ji Seun ; Kang, So Young ; Jeun, Yong Chull ;
Research in Plant Disease, volume 20, issue 2, 2014, Pages 101~106
DOI : 10.5423/RPD.2014.20.2.101
Citrus canker caused by Xanthomonas citri subsp. citri (Xcc) is one of the most important diseases on citrus. Although Satsuma mandarin cultivating mostly in Korea is moderately resistance to canker, occurrence of the disease were more frequently reported since last decade. Like other diseases in citrus, citrus canker was mainly protected by chemical fungicide in the field. Due to the side effect of the chemicals, alternative method of disease control is recently required. In this study four rhizobacterial strains TRH423-3, MRL408-3, THJ609-3 and TRH415-2 are selected by testing its antifungal activity against Xcc. Pre-inoculation with the selected rhizobacterial strains caused disease suppression on the citrus leaves after inoculation with the citrus canker pathogen. Similarly, in the field test symptoms of citrus canker were less developed in the citrus trees applied several times with the selected rhizobacterial strains compared with those of untreated trees. Therefore, it is suggested that the selected rhizobacterial strains may be valuable as an alternative method in the environment-friendly citrus farm.
Effect of Density of Helicotylenchus dihystera on Growth of Solanum lycopersicum
Kim, Donggeun ; Ryu, Younghyun ; Lee, Younsu ; Choi, Insoo ; Hu, Changsuk ;
Research in Plant Disease, volume 20, issue 2, 2014, Pages 107~111
DOI : 10.5423/RPD.2014.20.2.107
A greenhouse experiment was conducted to examine the effect of initial population density (Pi) of Helicotylenchus dihystera on six commercial tomato cultivars. Two-week-old tomato seedlings of six commercial cultivars were transplanted in d-10-cm clay pot and was inoculated with to give 0, 0.02, 0.2, and 2 nematodes/g soil. Plants were grown in a greenhouse for 60 days. Root and plant weights were unaffected but plant height declined only at inoculum level of 2 nematodes/g soil. At the highest initial population density (2 nematodes/g soil), plant height of tomato cv. Poseidon was reduced by 24%. Tomato cv. Hoyong produced the most nematodes with 7.0 nematodes/g soil and the least was tomato cv. Miniheuksu with 2.2 nematodes/g soil.
Control of the Root-Knot Nematode (Meloidogyne spp.) on Cucumber by a Liquid Bio-Formulation Containing Chitinolytic Bacteria, Chitin and Their Products
Ha, Woo Jong ; Kim, Young Cheol ; Jung, Hyuncha ; Park, Seur Kee ;
Research in Plant Disease, volume 20, issue 2, 2014, Pages 112~118
DOI : 10.5423/RPD.2014.20.2.112
A liquid bio-formulation containing chitinolytic bacteria, chitin and their products was assessed for its potential biological control against root-knot nematodes on cucumber. The bio-formulation was prepared by cultures of three chitinolytic bacteria, Chromobacterium sp. strain C-61, Lysobacter engymogenes and Serratia plymuthica in minimal medium supplemented with chitin. Under pot conditions, the bio-formulation showed better growth of cucumber plants, and less root galls and population density of Meloidogyne spp. than control media without the bio-formulation. In a greenhouse, 75-fold diluted bio-formulations were treated instead of water around cucumber plants through hoses for drip irrigation six times at 5-day intervals from the transplanting date. After 30 and 60 days, the treatment provided about 7% and 10% enhancement in the plant height and about 78% and 69% reduction in population density of Meloidogyne spp. in the rhizosphere, respectively. In addition, the experiments showed that the control effects occurred only in the soils contacted with the bio-formulation. Undiluted bio-formulations were drenched three times at 10-day intervals around cucumber plants severely infested with Meloidogyne spp. The treatment showed about 37% plant enhancement without dead plants compared with 37% death in the untreated control, and about 82% nematode reduction. These results suggest that the bio-formulation can be practically used to control the root-knot nematode on cucumber.
Development of Efficient Screening Methods for Resistant Cucumber Plants to Meloidogyne incognita
Hwang, Sung Min ; Jang, Kyoung Soo ; Choi, Yong Ho ; Kim, Jin-Cheol ; Choi, Gyung Ja ;
Research in Plant Disease, volume 20, issue 2, 2014, Pages 119~125
DOI : 10.5423/RPD.2014.20.2.119
Root-knot nematodes represent a significant problem in cucumber, causing reduction in yield and quality. To develop screening methods for resistance of cucumber to root-knot nematode Meloidogyne incognita, development of root-knot nematode of four cucumber cultivars ('Dragonsamchuk', 'Asiastrike', 'Nebakja' and 'Hanelbakdadaki') according to several conditions such as inoculum concentration, plant growth stage and transplanting period was investigated by the number of galls and egg masses produced in each seedling 45 days after inoculation. There was no difference in galls and egg masses according to the tested condition except for inoculum concentration. Reproduction of the nematode on all the tested cultivars according to inoculum concentration increased in a dose-dependent manner. On the basis of the result, the optimum conditions for root-knot development on the cultivars is to transplant period of 1 week, inoculum concentration of 5,000 eggs/plant and plant growth stage of 3-week-old in a greenhouse (
). In addition, under optimum conditions, resistance of 45 commercial cucumber cultivars was evaluated. One rootstock cultivar, Union was moderately resistant to the root-knot nematode. However, no significant difference was in the resistance of the others cultivar. According to the result, we suggest an efficient screening method for new resistant cucumber to the root-knot nematode, M. incognita.
The Comparison of Resistance of Sweet Potato Cultivars to Sclerotium Rot Caused by Sclerotium rolfsii
Kim, Shin-Chul ; Kim, Ju-Hee ; Cheong, Seong-Soo ; Choi, Kyu-Hwan ; Lee, Wang-Hyu ; Shim, Hong-Sik ;
Research in Plant Disease, volume 20, issue 2, 2014, Pages 126~128
DOI : 10.5423/RPD.2014.20.2.126
This study was carried out to assay the disease incidence degree of Sclerotium rot caused by Sclerotium rolfsii in sixteen most popular commercial cultivars of sweet potato (Ipomoea batatas) in Republic of Korea. The degree of disease incidence was evaluated on pot experiments. In pot experiments using artificial inoculation, the Sclerotium rolfsii caused a stem rot on seedling of sweet potato plants and causes a crown rot on lower stems near or at the soil line at favorable environmental conditions. White mycelial mats and sclerotia were formed at the infection sites. Plants severely infected were fell over or died because lower stems near soil surface were rotten. The degree of disease incidence was varied according to cultivars. Two cultivars, Bio-mi and Deayumi, were very resistant, while five cultivars, Shinjami, Shingeonmi, Hongmi, Yeonjami, and Shinhung-3 were highly susceptible.
First Report of Postharvest Gray Mold Rot on Carrot Caused by Botrytis cinerea in Korea
Aktaruzzaman, Md. ; Kim, Joon-Young ; Xu, Sheng-Jun ; Kim, Byung-Sup ;
Research in Plant Disease, volume 20, issue 2, 2014, Pages 129~131
DOI : 10.5423/RPD.2014.20.2.129
In February 2014, gray mold rotting symptoms were observed in carrots in cold storage at Gangneung, Gangwon province, Korea. The typical symptom of gray mold rot showed abundant blackish gray mycelia and conidia was observed on the infected root. The pathogen was isolated from infected root and cultured on PDA for further fungal morphological observation and confirming its pathogenicity according to Koch's postulates. Results of morphological data, pathogenicity test and rDNA internal transcribed spacer (ITS 1 and 4) sequence showed that the postharvest gray mold rot of carrot was caused by Botyrtis cinerea. This is the first report of postharvest gray mold rot on carrot in Korea.