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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Research in Plant Disease
Journal Basic Information
Journal DOI :
Korean Society of Plant Pathology
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Volume & Issues
Volume 20, Issue 4 - Dec 2014
Volume 20, Issue 3 - Sep 2014
Volume 20, Issue 2 - Jun 2014
Volume 20, Issue 1 - Mar 2014
Selecting the target year
A Review of Detection Methods for the Plant Viruses
Jeong, Joo-Jin ; Ju, Ho-Jong ; Noh, Jaejong ;
Research in Plant Disease, volume 20, issue 3, 2014, Pages 173~181
DOI : 10.5423/RPD.2014.20.3.173
The early and accurate detection of plant viruses is an essential component to control those. Because the globalization of trade by free trade agreement (FTA) and the rapid climate change promote the country-to-country transfer of viruses and their hosts and vectors, diagnosis of viral diseases is getting more important. Because symptoms of viral diseases are not distinct with great variety and are confused with those of abiotic stresses, symptomatic diagnosis may not be appropriate. From the last three decades, enzyme-linked immunosorbent assays (ELISAs), developed based on serological principle, have been widely used. However, ELISAs to detect plant viruses decrease due to some limitations such as availability of antibody for target virus, cost to produce antibody, requirement of large volume of sample, and time to complete ELISAs. Many advanced techniques allow overcoming demerits of ELISAs. Since the polymerase chain reaction (PCR) developed as a technique to amplify target DNA, PCR evolved to many variants with greater sensitivity than ELISAs. Many systems of plant virus detection are reviewed here, which includes immunological-based detection system, PCR techniques, and hybridization-based methods such as microarray. Some of techniques have been used in practical, while some are still under developing to get the level of confidence for actual use.
Identification and Characterization of Novel Biocontrol Bacterial Strains
Lee, Seung Hwan ; Kim, In Seon ; Kim, Young Cheol ;
Research in Plant Disease, volume 20, issue 3, 2014, Pages 182~188
DOI : 10.5423/RPD.2014.20.3.182
Because bacterial isolates from only a few genera have been developed commercially as biopesticides, discovery and characterization of novel bacterial strains will be a key to market expansion. Our previous screen using plant bioassays identified 24 novel biocontrol isolates representing 12 different genera. In this study, we characterized the 3 isolates showing the best biocontrol activities. The isolates were Pantoea dispersa WCU35, Proteus myxofaciens WCU244, and Exiguobacterium acetylicum WCU292 based on 16S rRNA sequence analysis. The isolates showed differential production of extracellular enzymes, antimicrobial activity against various fungal or bacterial plant pathogens, and induced systemic resistance activity against tomato gray mold disease caused by Botrytis cinerea. E. acetylicum WCU292 lacked strong in vitro antimicrobial activity against plant pathogens, but induced systemic resistance against tomato gray mold disease. These results confirm that the trait of biological control is found in a wide variety of bacterial genera.
Disease Occurrence in Transgenic Rice Plant Transformed with Silbene Synthase Gene and Evaluation of Possible Horizontal Gene Transfer to Plant Pathogens
Yu, Sang-Mi ; Jeong, Ui-Seon ; Lee, Ha Kyung ; Baek, So Hyeon ; Kwon, Soon Jong ; Lee, Yong Hoon ;
Research in Plant Disease, volume 20, issue 3, 2014, Pages 189~195
DOI : 10.5423/RPD.2014.20.3.189
Genetic engineering is being used to enhance disease resistance and nutritional value of crops including rice plant. Considering the fast-growing agricultural biotechnology and rapidly increasing global area of transgenic crops, the risk evaluation on environment is necessary. In this study, we surveyed the difference of disease occurrence between transgenic rice variety, Iksan526 transformed with peanut stilbene synthase gene and non-transgenic rice varieties, Dongjin and Nampyeong in the field. Moreover, the possibility of gene transfer from transgenic rice to bacterial and fungal pathogens was investigated. The results of this study indicated that there was no significant difference in the occurrence and severity of the diseases between Iksan526 and Dongjin or Nampyeong. In addition, the results suggested that rice pathogen, such as Xanthomonas oryzae pv. oryzae, Rhizoctonia solani and Magnaporthe grisea did not take up stilbene synthase and bar genes under natural conditions. Moreover the transformed DNA was not transferred to the pathogens even in repetitive contacts.
Improvement of Control Efficacy of Mancozeb Wettable Powder against Citrus Melanose by Mixing with Paraffin Oil
Yi, Pyoung-Ho ; Hyun, Jae-Wook ; Hwang, Rok-Yeon ; Kim, Kwang-Sik ;
Research in Plant Disease, volume 20, issue 3, 2014, Pages 196~200
DOI : 10.5423/RPD.2014.20.3.196
This study was carried out to determine the effect of mixing with paraffin oil on rainfastness of mancozeb on citrus fruits and assay the improvement of control effect of mancozeb against citrus melanose by mixing with paraffin oil. In artificial rainfall condition (7.2 mm/hr), the attached contents of mancozeb on detached fruits were the most in treatment of mancozeb 0.2% + paraffin oil 0.1% as the contents was
after treatment of rainfall for 10 hr. In open field condition, the contents of attached mancozeb on fruits were significantly more in treatment of mancozeb 0.2% + paraffin oil 0.1% or 0.25% than the other treatments 1, 15 and 25 days after treatment 2009 and 2010 seasons. The disease incidence was significantly lower in treatment of mancozeb 0.2% + paraffin oil 0.1% or 0.25% than treatment of mancozeb 0.2% only 2009, 2010 and 2011 seasons. Based on this study, it was suggested that the control effect of mancozeb against citrus melanose can be improved by mixing with paraffin oil.
Control Efficacy of Serenade Formulation against Rhizoctonia and Pythium Damping-off Diseases
Jo, Eun Ju ; Kang, Bum Gwan ; Jang, Kyoung Soo ; Choi, Yong Ho ; Kim, Jin-Cheol ; Choi, Gyung Ja ;
Research in Plant Disease, volume 20, issue 3, 2014, Pages 201~205
DOI : 10.5423/RPD.2014.20.3.201
Damping-off, caused by Rhizoctonia solani and Pythium ultimum, is a very important plant disease and affects seeds and seedlings of many plant species worldwide. To investigate control efficacy of Serenade formulation (1.34%, SC) against Rhizoctonia and Pythium damping-off diseases, seeds of chili pepper and cucumber were sown in soils inoculated with R. solani and P. ultimum and Serenade formulation was applied by soil-drenching with 9-, 19-, and 39-fold dilutions. Control values of Serenade formulation on Rhizoctonia damping-off of chili pepper was 58% and 29% for 9- and 19-fold dilutions, respectively. In the case of cucumber Rhizoctonia damping-off, Serenade treatments showed similar control efficacy with damping-off of chili pepper. On the other hand, control efficacy of Serenade formulation on Pythium damping-off of cucumber was less than control effects on Rhizoctonia damping-off. Only Pythium damping-off of chili pepper treated with 9-fold dilution Serenade was statistically different with untreated control. This result suggest that Serenade formulation could be effectively used for controlling Rhizoctonia and Pythium damping-off diseases.
Characteristics of Watermelon Mosaic Virus Transmission Occurring in Korean Ginseng
Choi, Seung-Kook ; Cho, In-Sook ; Chung, Bong-Nam ; Kim, Mi-Kyeong ; Jung, Won-Kwon ; Choi, Gug-Seoun ;
Research in Plant Disease, volume 20, issue 3, 2014, Pages 206~210
DOI : 10.5423/RPD.2014.20.3.206
Korean ginseng (Panax ginseng) is the most popular herb for medical purpose in Korea. Recently, viral diseases from Korean ginseng showing various degrees of severe mottling, variegation and mosaic symptoms have caused quantity losses of Korean ginseng in a large number of farms. Watermelon mosaic virus (named WMV-gin) was identified as a causal agent for the disease of Korean ginseng. Interestingly, WMV-gin failed to infect both Korean ginseng plant and susceptible host species including cucurbitaceous plants by mechanical inoculation. However, WMV-gin could successfully infect Korean ginseng by transmission of two aphid species (Myzus persicae and Aphis gossypii). It is likely that transmission of WMV-gin was done by both the aphid species during feeding behavior of the two aphid species on Korean ginseng, though the aphids dislike feeding in Korea ginseng. Similarly, a strain of WMV (WMV-wm) isolated from watermelon was transmitted successfully to Korean ginseng plant by the two aphid species, but not by mechanical inoculations. Transmission assays using M. persicae and A. gossypii clearly showed both WMV-gin and WMV-wm were not transmitted from infected Korean ginseng plant to cucurbit species that are good host species for WMV. These results suggest WMV disease occurring in Korean ginseng plant can be controlled by ecological approaches.
Protective Activity of the Mixtures of Pine Oil and Copper Hydroxide against Bacterial Spot and Anthracnose on Red Pepper
Soh, Jae-Woo ; Han, Kyung-Sook ; Lee, Seong-Chan ; Lee, Jung-Sup ; Park, Jong-Han ;
Research in Plant Disease, volume 20, issue 3, 2014, Pages 211~215
DOI : 10.5423/RPD.2014.20.3.211
This research was performed to examine the protective activities of the mixtures of pine oil and copper hydroxide against bacterial spot and anthracnose on pepper plants. As for bacterial spot, the treatment of pine oil alone displayed high disease incidence (59.6%) and low protective effect (28.9%). In comparison, the treatments of mixtures and copper hydroxide alone showed protective activities of 66.8-76.1%. The mixture of pine oil and copper hydroxide (4:1) suppressed the most effectively bacterial spot on pepper. On the other hand, the mixture of pine oil and copper hydroxide (4:1) also showed the strongest protective effect against pepper anthracnose among the 4 treatments tested; its disease incidence and disease control value were 49.8% and 41.7%, respectively. The other treatments showed low protective activities with control values of 7.4-17.1%. These results suggested that the mixture of pine oil and copper hydroxide (4:1) can be used for the environmental-friendly disease control of bacterial spot and anthracnose on pepper.
Draft Genome Sequence of a Chitinase-Producing Biocontrol Bacterium, Lysobacter antibioticus HS124
Gardener, Brian B. McSpadden ; Kim, In Seon ; Kim, Kil Yong ; Kim, Young Cheol ;
Research in Plant Disease, volume 20, issue 3, 2014, Pages 216~218
DOI : 10.5423/RPD.2014.20.3.216
Lysobacter antibiocus HS124 is a chitinase-producing rhizobacterium with proven capacities to suppress plant diseases. Bacterial cultures of L. antibioticus HS124 showed strong biocontrol efficacies against various plant diseases compared to those of bacterial cultures of Bacillus subtilis QST713 which is an active ingredient of a commercial biopesticide, Serenade. Here, we report the draft genome sequence and automated annotation of strain HS124. This draft genome sequence indicates the novelty of L. antibiocus HS124 and a subset of gene functions that may be related to its biocontrol activities.
Molecular Characterization of Tomato Yellow Leaf Curl Virus in Korea and the Construction of an Infectious Clone
Lee, Bong Choon ; Ueda, Shigenori ; Yoon, Young-Nam ; Shin, Dong Bum ; Kang, Hang-Won ;
Research in Plant Disease, volume 20, issue 3, 2014, Pages 219~222
DOI : 10.5423/RPD.2014.20.3.219
Several tomato production regions in Korea were surveyed for tomato yellow leaf curl disease (TYLCD). Tomato leaf samples showing TYLCD-like symptoms were collected from Tongyeong (To), Geoje (Gi), and Gimhae (Gh) cities of the southern part of Korea. Tomato yellow leaf curl virus (TYLCV) was detected and the full-length genomes of the isolates were sequenced. The TYLCV isolates found in Korea shared high sequence identity (> 99%) with TYLCV-IL [JR:Omu:Ng] (AB110217). Phylogenetic relationship analysis revealed that they formed two groups (with little genetic variability), and the To, Gj, and Gh isolates belonged to the TYLCV-IL group. An infectious clone of TYLCV-To (JQ013089) was constructed and agroinoculated into Nicotiana benthamiana, Nicotiana tabacum var. Xanthi, Petunia hybrida, Capsicum annuum, and Lycopersicon esculentum cv. Hausumomotaro. Agroinfection with a dimeric infectious clone of TYLCV-To induced severe leaf curling and stunting symptoms in these plants, excluding C. annuum. Tomato plants then developed typical yellow leaf curl symptoms.
Sclerotium Rot of Cyclamen europaeum Caused by Sclerotium rolfsii
Kwon, Jin-Hyeuk ; Lee, Heung-Su ; Kim, Jinwoo ; Kim, Won-Il ; Shim, Hong-Sik ; Shen, Shun-Shan ;
Research in Plant Disease, volume 20, issue 3, 2014, Pages 223~226
DOI : 10.5423/RPD.2014.20.3.223
Sclerotium rot caused by Sclerotium rolfsii occurred on Cyclamen europaeum grown at the experimental greenhouse of Gyeongsangnam-do Agricultural Research and Extension Services in April 2013. Infected plants showed water-soaked appearance, wilting, and rotting; severely infected plants eventually died. White mycelial mats spread over lesions, and sclerotia were formed on leaves, petioles, and flower stalks near soil line. On the basis of mycological characteristics, ITS rDNA sequence analysis, and pathogenicity to host plants, this fungus was identified as S. rolfsii Saccardo. This is the first report of sclerotium rot on C. europaeum caused by S. rolfsii in Korea.
Effect of Acetic and Lactic Acid Mixtures on Control of Quarantine Nematode, Bursaphelenchus xylophilus, in Exporting Cymbidium
Seo, Yunhee ; Park, Jiyeong ; Cho, Myoung Rae ; Chun, Jae Yong ; Kim, Young Ho ;
Research in Plant Disease, volume 20, issue 3, 2014, Pages 227~233
DOI : 10.5423/RPD.2014.20.3.227
The mixture (MX) of acetic acid (AA) and lactic acid (LA) was examined for its effectiveness in the control of the pine wood nematode Bursaphelenchus xylophilus contaminated in cymbidium culture medium. Nematode mortality in vitro was nearly 100% in AA and MX at the concentrations of 5.0-1.0% (pH 2.6 - 4.2) and in LA only at 5.0% (pH 3.5), but lowered at concentrations of 0.5-0.1% (pH 5.1-6.9) more significantly in LA than AA and MX. MX of most concentrations caused higher nematode mortality than the average response to AA and LA. All treatments of MX (0.5% and 0.25%), fosthiazate (standard and double concentrations) and culture dilution of Paenibacillus polymyxa GBR-1 (
colony-forming units/ml) reduced significantly the nematode populations in the cymbidium culture medium, compared to non-treatment control, with no significant difference among the treatments. No phytotoxicity occurred in all treatments. pH of the medium with the time after treatment and growths of 2-year-old cymbidium were not significantly different among treatments. Considering the safety and price of the organic acids, use of MX in the processes for culturing cymbidium may be a practically reliable and eco-friendly way in the control of the quarantine nematode in cymbidium.