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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Research in Plant Disease
Journal Basic Information
Journal DOI :
Korean Society of Plant Pathology
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Volume & Issues
Volume 20, Issue 4 - Dec 2014
Volume 20, Issue 3 - Sep 2014
Volume 20, Issue 2 - Jun 2014
Volume 20, Issue 1 - Mar 2014
Selecting the target year
Evaluation of Cabbage- and Broccoli-genetic Resources for Resistance to Clubroot and Fusarium Wilt
Lee, Ji Hyun ; Jo, Eun Ju ; Jang, Kyoung Soo ; Choi, Yong Ho ; Kim, Jin-Cheol ; Choi, Gyung Ja ;
Research in Plant Disease, volume 20, issue 4, 2014, Pages 235~244
DOI : 10.5423/RPD.2014.20.4.235
Clubroot and Fusarium wilt of cole crops (Brassica oleracea L.) are destructive diseases which for many years has brought a decline in quality and large losses in yields all over the world. The breeding of resistant cultivars is an effective approach to reduce the use of chemical fungicides and minimize crop losses. This study was conducted to evaluate the resistance of 60 cabbage (B. oleracea var. capitata) and 6 broccoli (B. oleracea var. italica) lines provided by The RDA-Genebank Information Center to clubroot and Fusarium wilt. To investigate resistance to clubroot, seedlings of the genetic resources were inoculated with Plasmodiophora brassicae by drenching the roots with a mixed spore suspension (1 : 1) of two isolates. Of the tested genetic resources, four cabbage lines were moderately resistant and 'K166220' represented the highest resistance to P. brassicae. The others were susceptible to clubroot. On the other hand, to select resistant plants to Fusarium wilt, the genetic resources were inoculated with Fusarium oxysporum f. sp. conglutinans by dipping the roots in spore suspension of the fungus. Among them, 17 cabbage and 5 broccoli lines were resistant, 16 cabbage lines were moderately resistant, and the others were susceptible to Fusarium wilt. Especially, three cabbage ('IT227115', 'K161791', 'K173350') and two broccoli ('IT227100', 'IT227099') lines were highly resistant to the fungus. We suggest that the resistant genetic resources can be used as a basic material for resistant B. oleracea breeding system against clubroot and Fusarium wilt.
Efficient Screening Method for Resistance of Cucumber Cultivars to Fusarium oxysporum f. sp. cucumerinum
Lee, Ji Hyun ; Kim, Jin-Cheol ; Jang, Kyoung Soo ; Choi, Yong Ho ; Choi, Gyung Ja ;
Research in Plant Disease, volume 20, issue 4, 2014, Pages 245~252
DOI : 10.5423/RPD.2014.20.4.245
The study was performed to establish an efficient screening method for resistant cucumber to Fusarium oxysporum f. sp. cucumerinum. The isolate KR5 was identified as F. oxysporum f. sp. cucumerinum based on molecular analyses of ITS and TEF genes and host-specificity test on cucurbits including melon, oriental melon, cucumber, and watermelon. Then four cucumber and two rootstock cultivars showing different resistance degrees to the Fusarium wilt pathogen KR5 were selected. And development of Fusarium wilt of the six cultivars according to several conditions, including incubation temperature after inoculation, inoculum concentration, root wounding, and growth stages of seedlings, was investigated. Disease severity of Fusarium wilt on the resistant cultivars was changed with incubation temperatures after inoculation. The resistant cultivars showed the higher resistance when inoculated plants were kept at 25 or
. Among four different growth stages of the seedlings, seven-day-old seedling represented the most difference of resistance and susceptibility to Fusarium wilt. From above results, we suggest that an efficient screening method for resistant cucumber to F. oxysporum f. sp. cucumerinum is to dip the non-cut roots of seven-day-old seedlings in spore suspension of
conidia/ml and to transplant the seedling into a non-infected soil, and then to incubate the inoculated plants in a growth room at
for 3 weeks to develop Fusarium wilt.
Resistance Evaluation of Tomato Germplasm against Bacterial Wilt by Ralstonia solanacearum
Jung, Eun Joo ; Joo, Hae Jin ; Choi, Soo Yeon ; Lee, Seung Yeup ; Jung, Yong Hoon ; Lee, Myung Hwan ; Kong, Hyun Gi ; Lee, Seon-Woo ;
Research in Plant Disease, volume 20, issue 4, 2014, Pages 253~258
DOI : 10.5423/RPD.2014.20.4.253
This study was conducted to evaluate tomato plant resistance against bacterial wilt by Ralstonia solanacearum using tomato cultivars or tomato breeding lines maintained in RDA-Genebank of Rural Development Administration and to select resistant tomato lines for breeding purpose. We evaluated the disease responses of a total of 13 cultivars and 39 breeding lines from RDA-Genebank using R. solanacearum SL341 strain, which is a representative strain in Korea. Tomato cultivar Hawaii 7996 and Moneymaker were used as a resistant control plant and a susceptible control plant, respectively. A total of 32 cultivars were susceptible and 10 cultivars showed various disease response suggesting resistant phenotype segregation in the lines. Five commercial cultivars and 5 breeding lines exhibited strong resistance to bacterial wilt by the SL341 strain. These 5 breeding lines might be used for further study of plant defense response against bacterial wilt and cloning of the resistance gene from tomato plants. Ultimately, the selected lines could be used for tomato breeding to generate bacterial wilt resistant tomato plants.
Use of Sodium Hypochlorite for the Control of Bakanae Disease in Rice
Shin, Dong Bum ; Goh, Jaeduk ; Lee, Bong Choon ; Kang, In Jeong ; Kang, Hang-Won ;
Research in Plant Disease, volume 20, issue 4, 2014, Pages 259~263
DOI : 10.5423/RPD.2014.20.4.259
For application of sodium hypochlorite as a seed disinfectant to the control of bakanae disease caused by Gibberella fujikuroi in rice, we investigated the effects of sodium hypochlorite for antifungal activity, eliminating fungus from seeds and reducing disease occurrence in vitro and greenhouse. The viability of the pathogen was significantly reduced at
concentration of sodium hypochlorite, and the pathogens did not grow at over
concentration of sodium hypochlorite. The effect of eliminating fungus was 90% at treatment of 0.3% sodium hypochlorite solution to infected rice seeds for eight hours. When the rice seeds were soaked into 0.5% and 0.3% sodium hypochlorite solutions for twelve hours, the disease incidences of rice seedling were remarkably reduced to 4.3% and 4.7%, respectively, compared to 97.3% of non-treatment control. The rates of seedling stand were 29.1% and 26.9% higher with the sodium hypochlorite treatment than that of non-treatment control. When prochloraz and sodium hypochlorite was treated to naturally severely infested rice seeds with bakanae disease, the disinfection effect was higher than that of prochloraz alone treatment. When the seeds were soaked in sodium hypochlorite before or after prochloraz, the rate of seed contamination was low as 4.0% or 6.3%, respectively, compared to prochloraz alone as 13.7%. The disease incidence was low as 3.7% or 8.3%, respectively, compared to prochloraz alone as 14.3%. The disinfection effect of treatment with prochloraz after sodium hypochlorite was higher than that of treatment with prochloraz before sodium hypochlorite.
Control Effect of Sudan Grass on Root-Knot Nematode, Meloidogyne incognita, in Cucumber and Lettuce Greenhouses
Kim, Hyeong-Hwan ; Kim, Dong-Hwan ; Yang, Chang-Yeol ; Kang, Taek-Jun ; Han, Kyung-Sook ; Park, Hae-Woong ; Jung, Young-Hak ; Jeon, Sung-Wook ; Song, Jin-Sun ; Choo, Ho Yul ;
Research in Plant Disease, volume 20, issue 4, 2014, Pages 264~269
DOI : 10.5423/RPD.2014.20.4.264
The effect of biological control of the root-knot nematode, Meloidogyne incognita, on cucumber and lettuce was evaluated with green manure crop species in greenhouse. Nematicidal effect of sudan grass cultivation in cucumber greenhouse was comparable to that of chemical treatment with fosthiazate GR, showing the high activity of 88.6%. Sudan grass cultivation in lettuce greenhouse significantly reduced the number of M. incognita in soil, showing 93.5% of nematiidal activity. In addition, since growth of sudan grass was superior to other green manure crop species, it is considered that cultivation of sudan grass is proper to control M. incognita in greenhouse.
Assessment of Virulence of Raffaelea quercus-mongolicae and Raffaelea spp. Isolates by Artificial Inoculation of Quercus mongolica Logs
Son, Su-Yeon ; Seo, Sang-Tae ; Park, Hyung-Cheol ;
Research in Plant Disease, volume 20, issue 4, 2014, Pages 270~274
DOI : 10.5423/RPD.2014.20.4.270
Division of Forest Diseases & Insect Pests, Korea Forest Research Institute, Seoul 130-712, Korea The objective of this study was to evaluate the virulence of geographically different isolates of oak wilt pathogen, Raffaelea quercus-mongolicae and other Raffaelea species. In this study, mature trees of Quercus mongolica were inoculated with the various isolates of Raffaelea spp. and their virulence was evaluated by measuring the extent of sapwood discoloration resulting from the inoculation. The average length of discolored sapwood in a lateral direction was longest in the trees inoculated with the isolates from Korea (8.69 cm) followed by R. quercivora (7.51 cm) and the other Raffaelea spp. (3.35 cm). The lateral length of discolored sapwood caused by the inoculation with Korean strains varied from 4.71 to 14.90 cm indicating their differences in virulence. The area of discolored sapwood caused by the inoculation with Raffaelea spp. varied from 1.57 to
indicating their differences in virulence. Based on the length and area of the discolored sapwoods, isolated YY and wj43 appeared to have the highest virulence among all the Raffaelea isolates tested. Each of the two isolates was obtained from Gangwon Province and Jeonbuk Province, respectively.
Post-harvest Decay of 'Campbell Early' Grape
Noh, Young-Hee ; Kim, Yong-Eon ; Song, Min-Ji ; An, Ji-Hye ; Jeong, Min-Jung ; Hong, Seung-Beom ; Kim, Seon-Hwa ; Lee, Hyok-In ; Cha, Jae-Soon ;
Research in Plant Disease, volume 20, issue 4, 2014, Pages 275~282
DOI : 10.5423/RPD.2014.20.4.275
The occurrence of post-harvest diseases and their pathogens in 'Campbell Early' which is the most produced grape in Korea was investigated. The 'Campbell Early' grapes produced in 3 main grape-producing areas were stored in a cold room (
) for 2 weeks then at room temperature for 4 weeks prior to investigation. The major post-harvest diseases occurred were gray mold, blue mold, ripe rot, new decay 1, and new decay 2. Pathogens isolated from the symptoms were identified as Botrytis cinerea for gray mold, Penicillium sclerotiorum for blue mold and Collectrichum acutatum for ripe rot. Pathogens for new decay 1 and new decay 2 were not identified yet. Incidences of new decay 1 and new decay 2 were much higher than the other 3 decays in all grapes produced from 3 areas. Gray mold and blue mold occurred at much lower frequencies than these two decays, and ripe rot occurred least.
Inactivation of Xanthomonas citri subsp. citri and Effect on Infection of Citrus Canker by Gamma Irradiation
Kim, Kyung Nam ; Song, Min A ; Han, Sang Heon ; Song, Seong Joon ; Jeun, Yong Chull ;
Research in Plant Disease, volume 20, issue 4, 2014, Pages 283~288
DOI : 10.5423/RPD.2014.20.4.283
Citrus canker caused by Xanthomonas citri subsp. citri (Xcc) has been quarantined by many countries in the world. Recently, the usage of methyl bromide should be limited, application by gamma irradiation on the agricultural production is raised as an alternative method. In this study, the level of gamma irradiation which could decrease of population of Xcc in the suspension or on the surface of citrus fruit was investigated. The
value of Xcc, which is radiation dose required to reduce the number of the microorganism, was 55 and 28 Gy in the suspension and on the surface of citrus fruit, respectively. Furthermore, disease severity was suppressed on the citrus leaves inoculated with Xcc suspension pre-treated with gamma irradiation. Based on this study, it is suggested that Xcc on the citrus fruit could be eradicated by gamma irradiation and the results of this study may be valuable for application of gamma ray in quarantine activity.
Occurrence of Fungi and Fusarium Mycotoxins in the Rice Samples from Rice Processing Complexes
Lee, Theresa ; Lee, Soohyung ; Kim, Lee-Han ; Ryu, Jae-Gee ;
Research in Plant Disease, volume 20, issue 4, 2014, Pages 289~294
DOI : 10.5423/RPD.2014.20.4.289
Rice samples including paddy, husk, brown rice, blue-tinged rice, broken rice, discolored rice and polished rice were collected from rice processing complexes(RPC) nationwide to determine the contamination of fungi and mycotoxins on rice during 2010-2013. Among the samples, paddy rice had the highest frequencies of fungal and Fusarium occurrence, and the frequencies decreased along with milling as husk was the next. Blue-tinged rice or discolored rice was similar with brown rice for fungal occurrence, and polished rice showed the lowest frequency. Among Fusarium species, F. graminearum species complex occupied 87% in 2012 but did 35-39% in 2011 and 2013. Aspergillus and Penicillium species appeared at low frequency in most samples but occurred at higher frequency in certain RPC samples. Alternaria, Nigrospora, and Epicoccum species occurred similarly to the pattern of total fungi. The rice samples from 2010-2012 were analyzed for the occurrence of Fusarium mycotoxins including deoxynivalenol, nivalenol, zearalenone, and other trichothecenes. The most highly contaminated sample was discolored rice in terms of frequency, level, ratio of simultaneous contamination with multiple toxins, followed by blue-tinged rice.
Control Efficacy of Fungicide Injection on Oak Wilt in the Field
Son, Su-Yeon ; Seo, Sang-Tae ; Park, Ji-Hyun ;
Research in Plant Disease, volume 20, issue 4, 2014, Pages 295~298
DOI : 10.5423/RPD.2014.20.4.295
Oak wilt caused by Raffaelea quercus-mongolicae was first noticed in South Korea in 2004 and, ever since, its distribution and damage have been increasing. To screen a fungicide effective for oak wilt control by tree injection, laboratory and field experiments were conducted. Ten fungicides and one antibiotic were examined in vivo for their effectiveness in restricting the growth of R. quercus-mongolicae and R. quercivora (Japanese oak wilt pathogen) isolates. To the Korean isolates of R. quercus-mongolicae, chlorothalonil showed the highest fungicidal effects, followed by benomyl and propiconazole. To the Japanese one, propiconazole was highest in the fungicidal effectiveness, followed by benomyl and bitertanol. Propiconazole was selected for field-testing of its control efficacy because it showed good fungicidal effects in vitro and systemic activity. The control efficacy in the field was 87.5% in the first year of injection and 66.7% in the second year, indicating the fungicidal effects last at least over one year.
Resistance Screening to Pepper mild mottle virus Pathotypes in Paprika Cultivars
Choi, Gug-Seoun ; Choi, Seung-Kook ; Cho, In-Sook ; Kwon, Sun-Jung ;
Research in Plant Disease, volume 20, issue 4, 2014, Pages 299~302
DOI : 10.5423/RPD.2014.20.4.299
The Paprika plant infected with Pepper mild mottle virus (PMMoV) do not produce commercial fruit as causing necrotic spots symptom on the fruit. Ten cultivars of paprika were analyzed to select the resistance cultivars against PMMoV pathotypes,
, using bioassay and genetic markers.
genotypes expressing resistance to the pathotypes existed in those cultivars but
genotype did not.
in cvs. Easy and Magnifico,
in cvs. Scirocco and Orange glory F1,
in cv. Special F1,
in cvs. Fiesta, Piero and Derby, and
in Cupra and Mazzona F1 were identified with SCAR and CAPS markers. The resistant cvs. to the 2 pathotypes were Magnipico, Easy, Scirocco F1, Orange glory and Special F1 and the susceptible cvs. were Fiesta, Piero, Derby, Cupra and Mazzona F1. The susceptible cvs. of the absence of
genotype showed systemic infection when inoculated with PMMoV-
. However, those cvs. despite the presence of
genotype showed vein necrosis on the inoculated leaf and hypersensitive necrosis symptom on the upper parts when inoculated with PMMoV-
Pattern of the Occurrence of Tomato yellow leaf curl virus on Cultivation
Ko, Sug-Ju ; Choi, Duck-Soo ; Ma, Kyung-Cheol ; Kim, Do-Ik ; Kim, Mi-Kyeong ; Choi, Hong-Soo ; Kim, Hyun-Woo ;
Research in Plant Disease, volume 20, issue 4, 2014, Pages 303~306
DOI : 10.5423/RPD.2014.20.4.303
Tomato yellow leaf curl virus (TYLCV) disease survey of tomato plants cultivated at Youngam, Changseong and Hwasun in Jeonnam Province was conducted in 2012 to 2013. To detect TYLCV in the field, PCR assay was performed using genomic DNAs extracted from tomato samples. According to cropping pattern, 23 and 59.4% of TYLCV infections were shown in semi-forcing and retarding cropping systems, respectively, and the frequencies of diseased plants were 0.4 and and 13.1% in the two cropping systems, respectively. Especially, TYLCV incidences in the susceptible varieties were 24.3 and 83.0% in semi-forcing and retarding cropping systems, respectively. Resistant varieties showed 9.1% infection in retarding cropping system but did not in semi-forcing cropping system. Also, TYLCV was occurred on early June after transplanting on April and at approximate 20 days after transplanting on August or September.
Characterization of Brugmansia mosaic virus Isolated from Brugmansia spp. in Korea
Park, Chung Youl ; Kim, Bong-Sub ; Nam, Moon ; Lee, Min-A ; Baek, Da-Som ; Bae, Yang Su ; Park, Eun-Hye ; Kim, Jeong-Sun ; Choi, Jong-Yoon ; Lim, Seungmo ; Moon, Jae Sun ; Lee, Su-Heon ;
Research in Plant Disease, volume 20, issue 4, 2014, Pages 307~313
DOI : 10.5423/RPD.2014.20.4.307
In May 2013, an angel's trumpet leaves showing mosaic and malformation symptoms were collected from Suwon city, Gyeonggi-do. An analysis of the collected sample by transmission electron microscopy observation showed filamentous rod particles of 720-800 nm in length. On the basis of the these observations, we performed PCR against three reported Potyviruses (Brugmansia mosaic virus, Colombian datura virus and Brugmansia suaveolens mottle virus), and the sample was positive for BruMV. Pathogenicity and host range test of BruMV was determined by mechanical inoculation. Solanaceae (tobacco, tomato and eggplant) and Amaranthaceae (ground cherry) appeared typical virus symptoms. To determine coat protein of this virus, we designed specific primer pairs, and performed PCR amplification, cloning, and sequencing. Phylogenetic analysis showed that BruMV-SW was most closely related to BruMV isolate SK. Comparison of the BruMV-SW coat protein nucleotide sequences showed 92% to 99% identities to the other BruMV isolates.