Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Research in Plant Disease
Journal Basic Information
Journal DOI :
Korean Society of Plant Pathology
Editor in Chief :
Volume & Issues
Volume 21, Issue 4 - Dec 2015
Volume 21, Issue 3 - Sep 2015
Volume 21, Issue 2 - Jun 2015
Volume 21, Issue 1 - Mar 2015
Selecting the target year
PCR-based Assay for the Specific Detection of Pseudomonas syringae pv. tagetis using an AFLP-derived Marker
Song, Eun-Sung ; Kim, Song-Yi ; Chae, Soo-Cheon ; Kim, Jeong-Gu ; Cho, Heejung ; Kim, Seunghwan ; Lee, Byoung-Moo ;
Research in Plant Disease, volume 21, issue 1, 2015, Pages 1~5
DOI : 10.5423/RPD.2015.21.1.001
A PCR method has been developed for the pathovar-specific detection of Pseudomonas syringae pv. tagetis, which is the causal agent of bacterial leaf spots and apical chlorosis of several species within the Compositae family. One primer set, PSTF and PSTR, was designed using a genomic locus derived from an amplified fragment length polymorphism (AFLP) fragment produced a 554-bp amplicon from 4 isolates of P. syringae pv. tagetis. In DNA dot-blot analysis with the PCR product as probe, a positive signal was identified in only 4 isolates of P. syringae pv. tagetis. These results suggest that this PCR-based assay will be a useful method for the detection and identification of P. syringae pv. tagetis.
Yield Loss Assessment and Determination of Control Thresholds for Anthracnose on Red Pepper
Kim, Ju-Hee ; Cheong, Seong-Soo ; Lee, Ki-Kwon ; Yim, Ju-Rak ; Choi, Sun-Woo ; Lee, Wang-Hyu ;
Research in Plant Disease, volume 21, issue 1, 2015, Pages 6~11
DOI : 10.5423/RPD.2015.21.1.006
This study was carried out to develop control thresholds for anthracnose on red pepper. To investigate relationship between anthracnose incidence and diseased fruits, experimental plots with six treatments were established. There existed close correlation between rate of diseased fruits and yields in field (Y = -16.83X + 327.57,
), in rain shelter (Y = -4.92X + 361.02,
), and in rain shelter with rain (Y = -5.91X + 359.71,
). Control thresholds for anthracnose of pepper were 0.9% diseased fruits per plant in field and from 1.7 to 2.3% diseased fruits rate per plant in rain shelter.
Antimicrobial Activity of Nano Materials against Acidovorax citrulli and Other Plant Pathogens
Kim, Sang Woo ; Adhikari, Mahesh ; Yadav, Dil Raj ; Lee, Hyun Goo ; Um, Young Hyun ; Kim, Hyun Seung ; Lee, Youn Su ;
Research in Plant Disease, volume 21, issue 1, 2015, Pages 12~19
DOI : 10.5423/RPD.2015.21.1.012
Antimicrobial activities of nano-materials were tested against several plant pathogens. Twelve different nano-materials were used to observe the antagonistic activity against three kinds of mold and sixteen different kinds of watermelon fruit rot pathogens (Acidovorax citrulli). According to the results, no antagonism have been found against the pathogen, Cylindrocarpon destructans. However in the case of Pythium ultimum, combination of Brass/Glucose 1,000 ppm confirmed the mycelial growth reduction by 94%. In addition, little effect was found against Rhizoctonia solani by Ag/Glucose 3,000 ppm. The remaining other nano-materials have different antimicrobial effect depending on the strains of A. citrulli. But in the case of lime (Cu/Salt 1,000 ppm) highest antimicrobial activity was observed with 97%. Moreover growth of five different strains of A. citrulli was checked by 99% with the combination of Ag/Glucose 1,000 ppm. 92% reduction of A. citrulli growth was observed with
3,000 ppm. Tested nano-materials against different plant pathogens in this study showed the antimicrobial activity at the range of 24-70%.
Evaluation of Sequential Planting Method for Screening of Durable Resistance against Rice Blast in Rice Breeding Program
Goh, Jaeduk ; Kim, Byoung-Ryun ; Shin, Dong Bum ; Kang, In Jeong ; Lee, Bong Choon ; Kang, Hang-Won ; Han, Seong-Sook ;
Research in Plant Disease, volume 21, issue 1, 2015, Pages 20~23
DOI : 10.5423/RPD.2015.21.1.020
A sequential planting method was developed to screen rice plants with durable resistance against rice blast in a short time, and applied for several years in Korean rice breeding program. In this study, we showed the advantages of a sequential planting method compared to other pathogenicity tests. The correlation analysis among three pathogenicity tests and other factors demonstrated that durable resistance depended on the average of diseased leaf area and the number of compatible pathogens. Significant correlations were found in the nursery test but not in the field test result. In addition, we traced changes in the pathogen population during sequential planting stages through re-isolation of the pathogen. The portion of compatible pathogens was increased during sequential planting. Through this study, we provide an effective sequential planting method and direction of durable resistance in a breeding program.
First Report of Fusarium Wilt of Fallopia multiflora Caused by Fusarium oxysporum in Korea
Park, Jong-Han ; Han, Kyung-Sook ; Lee, Seong-Chan ; Soh, Jae-Woo ; Park, Mi-Jeong ;
Research in Plant Disease, volume 21, issue 1, 2015, Pages 24~26
DOI : 10.5423/RPD.2015.21.1.024
In April 2014, seedlings of Fallopia multiflora showing wilt symptom were first found at a greenhouse in Punggi-eup, Yeongju-si, Korea. A Fusarium-like fungus was isolated from the wilted plant and it was identified as Fusarium oxysporum based on morphological characteristics and nucleotide sequence data of translation elongation factor 1-
. The fungus isolated from the diseased plant was revealed to be pathogenic to the host plant through pathogenicity tests, and the reisolation of the pathogen confirmed Koch's postulates. This is the first report of Fusarium wilt occurring on Fallopia multiflora in the world.
First Report of Fusarium Wilt Caused by Fusarium oxysporum on Kohlrabi in Korea
Choi, In-Young ; Kim, Ju ; Ju, Ho-Jong ; Park, Ji-Hyun ; Shin, Hyeon-Dong ;
Research in Plant Disease, volume 21, issue 1, 2015, Pages 27~31
DOI : 10.5423/RPD.2015.21.1.027
In April 2014, Fusarium wilt was found on kohlrabi seedlings in Iksan, Korea. Symptoms included wilting of foliage, drying and withering of older leaves, and stunting of the plants. The infected plants eventually died during growth. Colonies on potato dextrose agar were pinkish white, and felted with cottony and aerial mycelium. Macroconidia were falcate to almost straight, thin walled and usually 3-septate. Microconidia were usually formed abundantly in false-heads on short monophialides on the hyphae and were hyaline, smooth, oval to ellipsoidal, aseptate or medianly 1-septate, very occasionally 2-septate, slightly constricted at the septa,
. On the basis of the morphological characteristics and phylogenetic analyses of molecular markers (internal transcribed spacer rDNA and translation elongation factor
), the fungus was identified as Fusarium oxysporum. Pathogenicity of a representative isolate was proved by artificial inoculation, fulfilling Koch's postulates. To our knowledge, this is the first report on the occurrence of Fusarium oxysporum on kohlrabi in Korea.
First Report of Anthracnose on Bitter Gourd Caused by Colletotrichum gloeosporioides in Korea
Kim, Ju-Hee ; Kim, Ju ; Choi, In-Young ; Cheong, Seong-Soo ; Uhm, Mi-Jeong ; Lee, Wang Hyu ;
Research in Plant Disease, volume 21, issue 1, 2015, Pages 32~35
DOI : 10.5423/RPD.2015.21.1.032
Anthracnose occurred in bitter gourd grown in Jeongup areas of Korea in 2011. Anthracnose of bitter gourd appeared as dark brown circular spots on naturally infected leaves and fruits. The symptoms of infected leaves and fruits were small brown to dark brown spots and gradually enlarged to larger cylindrical dark brown lesions. The causal fungus of anthracnose isolated from the diseased plants was identified as Colletotrichum gloeosporioides based on the morphological and cultural characteristics and ITS rDNA sequence analysis. All isolates of C. gloeosporioides produced symptoms on the host leaves by artificial inoculation. This is the first report of anthracnose on bitter gourd caused by C. gloeosporioides in Korea.
Stem Rot on Ligularia fischeri Caused by Sclerotium rolfsii in Korea
Moon, Youn-Gi ; Kim, Se-Won ; Choi, Jun-Keun ; Kwon, Soon-Bae ; Shim, Hong-Sik ; Ju, Ho-Jong ; Choi, In-Young ;
Research in Plant Disease, volume 21, issue 1, 2015, Pages 36~39
DOI : 10.5423/RPD.2015.21.1.036
In June 2012 and 2013, a destructive stem rot symptoms of Ligularia fischeri occurred sporadically in Hoengseong-gun and Pyeongchang-gun Gangwon-do, Korea. The typical symptom included water-soaking on the main stem, rotting, wilting and blighting, which eventually leads to death of the plant. White mycelial mats were spread over lesions and brown sclerotia were formed on stems and near soil surface. The sclerotia were white to brown, spherical or irregular, 1-3 mm in size on potato dextrose agar (PDA), The optimum temperature range of hyphal growth was
and the hyphal diameter was
. The typical clamp connections were observed in the hyphae of the fungus grown on PDA. The resulting sequence of 695 bp was deposited in GenBank. A BLAST search revealed that sequences of the this isolates showed >99% identity with those of Sclerotium rolfsii. On the basis of the morphological characteristics and phylogenetic analyses of molecular markers ITS rDNA, the fungi were identified as S. rolfsii. A pathogenicity test was carried out to fulfill Koch's postulates. To our knowledge, this is the first report of S. rolfsii on Ligularia fischeri in Korea.
Hairs as Physical Barrier against Adhesion of Xanthomonas axonopodis pv. glycines on Soybean Leaf
Kim, Seung-Han ; Park, Seuk-Hee ; Woo, Jin-Ha ; Choi, Sung-Young ;
Research in Plant Disease, volume 21, issue 1, 2015, Pages 40~43
DOI : 10.5423/RPD.2015.21.1.040
Bacterial pustule of soybean is caused by Xanthomonas axonopodis pv. glycines, one of the most important diseases in soybean. The symptom of bacterial pustule is mainly distributed around leaf veins. However, the reason has not been known. In order to determine pathosystem of bacterial pustule in leaf, soybean leaves were collected and observed using scanning electron microscopy (SEM) and light microscopy. Many hairs were observed at abaxial sides of the leaf, few hairs were observed at tissue around the leaf veins. In addition, unidentified bacterial cells and dusts at the no hair part near veins were observed. In the inoculation assays, the cells of X. axonopodis pv. glycines were observed near leaf veins. The imprint of underside of soybean leaves inoculated with X.axonopodis pv. glycines on PDA showed that the growth of bacteria around veins was observed but no bacterial growth at the part with leaf hairs. Our data demonstrated that soybean leaf hairs play an important role as a physical barrier for structural resistance of soybean against bacterial pustule pathogen.