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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Research in Plant Disease
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Korean Society of Plant Pathology
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Volume & Issues
Volume 22, Issue 2 - Jun 2016
Volume 22, Issue 1 - Mar 2016
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Observation of Growth Inhibition of Elsinoe fawcettii on Satsuma Mandarin Leaves Pre-treated with Rhizobacterial Strains by a Scanning Electron Microscope
Park, Jae Sin ; Song, Min-A ; Jeun, Yong Chull ;
Research in Plant Disease, volume 22, issue 1, 2016, Pages 1~8
DOI : 10.5423/RPD.2016.22.1.1
Elsinoe fawcettii causing citrus scab was suppressed by rhizobacterial strains such as Burkholderia gladioli MRL408-3, TRH423-3 and Pseudomonas fluorescens THJ609-3, TRH415-2 having antifungal activity. The leaf surface of Satsuma mandarin, which was pre-treated with the rhizobacterial strains, was observed by a scanning electron microscope (SEM) after inoculation with E. fawcettii. The number of lesions was reduced on the leaves pre-treated with the rhizobacterial strains compared to those of untreated leaves. Especially, the lesions numbers was apparently reduced on the leaves pre-treated with B. gladioli MRL408-3. The observation by SEM revealed that not only the germination rate but also the length of germ tube of the pathogen were decreased on the rhizobacterial strains pre-treated leaves. These inhibition of the fungal growth was more strongly expressed on the leaves pre-treated with commercial fungicide imibenconazole, by which the lesions was rarely found on the leaves. Based on these results, it was suggested that rhizobacterial strains may inhibit the germination and growth of the E. fawcettii on the surface of citrus leaves, resulting in decrease of disease severity.
Distribution of Bacterial Angular Leaf Spot of Strawberry and Characterization of Xanthomonas fragariae Strains from Korea
Yoon, Myung-Ju ; Myung, Inn-Shik ; Lee, Jae-Yeon ; Kim, You-Shin ; Lee, Yong-Hwan ; Kim, Dae-Young ; Lee, Young-Ki ;
Research in Plant Disease, volume 22, issue 1, 2016, Pages 9~17
DOI : 10.5423/RPD.2016.22.1.9
Nationwide survey for angular leaf spot (ALS) of strawberry caused by Xanthomonas fragariae, a quarantine disease in Korea, was performed in November 2012. In the survey, ALS was observed in eighty eight farmers` fields of Sukok, Jinju and Okjong, Hadong in Gyeongnam Province, and one field in Namwon of Jeollabuk Province. The infected field of Namwon closed immediately after the disease diagnosed ALS. In detailed survey of Sukok and Okjong areas during February 2012 to January 2015, ALS occurrence decreased from 45% farmer`s fields on December 2012 to 5% on January 2015, and from 38% on November 2013 to 5% on January 2015, respectively. Phenotypic characteristics of the Korean strains were similar to those of the type strain of X. fragariae. A multilocus sequence analysis of Korean strains of X. fragariae was conducted using four genes; dnaK, fyuA, gyrB, and rpoD. All the Korean strains had the same sequences of the four genes. The concatenated sequences of the Korean strains shared 100% with that of the type strain of X. fragariae. All strawberry cultivars tested were susceptible to the strains of X. fragariae two weeks after inoculation. The inoculated sites were necrosis and expanded, which were rated 4 based on evaluation of inoculation site.
Screening Methods for Resistant Cucumber Cultivars against Cucumber Scab Caused by Cladosporium cucumerinum Using Cucumber Fruits and Seedlings
Park, So-Hyang ; Hong, Sung-Jun ; Shim, Chang-Ki ; Kim, Min-Jeong ; Park, Jong-Ho ; Han, Eun-Jung ; Park, Jong-Won ; Jee, Hyeong-Jin ; Kim, Seok-Cheol ; Kim, Yong-Ki ;
Research in Plant Disease, volume 22, issue 1, 2016, Pages 18~24
DOI : 10.5423/RPD.2016.22.1.18
This study was conducted to elucidate the cultural and pathogenic characteristics of Cladosporium cucumerinum PT1 and resistance of 81 commercial cucumbers (Cucumis sativus). Cucumber leaves and fruits appeared as scab were collected from a plastic film house located in Pyeongtaek, Gyeonggi-province, Korea in late March, 2015. A casual fungus was isolated from the diseased fruits on potato dextrose agar and it was identified as C. cucumerinum PT1 based on the morphological characteristics. To find out the effect of wounding and fruit size on the development of cucumber scab, small (<10 cm long), medium (10 to 20 cm long), and large (>20 cm long, commercially mature fruit) size cucumber fruits were harvested, C. cucumerinum PT1 pathogens were inoculated with a single droplet of suspension (
spores/ml) on wounded or unwounded cucumber fruits. Small fruits were completely damaged with showing severe water-soaking symptoms and fast pathogen growth regardless of wounded or unwounded. Meanwhile slight water-soaking symptoms on medium and large size fruits occurred and disease development into plant tissues was observed only on wounded fruits. Disease resistance of 81 commercial cucumber cultivars was evaluated on third-stage seedlings and small fruits by inoculating suspension (
spores/ml) of C. cucumerinum PT1. As a result, mini and pickling cultivar groups were resistant, `Cheoeumcheoreom` cultivar was symptomless and the other cultivars were resistant to medium resistant. On the other hand, most of cucumber cultivars belonging to the other groups were susceptible. Disease resistance of cucumber against cucumber scab was significantly different among cultivars and a few cucumber cultivars showed different disease resistant responses to two bioassay methods using seedlings and small fruits. Therefore, to screen scab resistance in cucumber, a test using both fruits and seedlings is advisable. We think that the selected resistant cultivars can be used to control cucumber scab effectively under the farmhouse condition.
Phylogenetic Analyses of Pepper mild mottle virus and Cucumber mosaic virus Isolated from Rorippa palustris
Kwon, Sun-Jung ; Yoon, Ju-Yeon ; Cho, In-Sook ; Choi, Seung-Kook ; Choi, Gug-Seoun ;
Research in Plant Disease, volume 22, issue 1, 2016, Pages 25~31
DOI : 10.5423/RPD.2016.22.1.25
During a field survey in 2014, a Rorippa palustris plant showing virus-like symptom was collected from a pepper field in Dangjin in Korea. The collected sample was subjected to examine infection with pepper-infecting viruses. Molecular diagnosis assay showed that the collected R. palustris sample was co-infected with Pepper mild mottle virus (PMMoV) and Cucumber mosaic virus (CMV). This is the first identification of PMMoV from R. palustris and the first report of CMV infection of R. palustris in Korea. To examine phylogenetic positions of the identified PMMoV and CMV isolates, their complete genome sequences of were determined and compared with those of previously reported isolates of the cognate viruses. Phylogenetic analyses revealed the isolates of PMMoV and CMV obtained from R. palustris are closely related to the pepper isolates of the cognate viruses. Our results suggest that R. palustris could act a weed reservoir of PMMoV and CMV.
In Vitro Expression and Antibody Preparation of Rice black-streaked dwarf virus Coat Protein Gene
Lee, Bong Choon ; Cho, Sang-Yun ; Bae, Ju Young ; Kim, Sang Min ; Shin, Dong Bum ; Kim, Sun Lim ;
Research in Plant Disease, volume 22, issue 1, 2016, Pages 32~37
DOI : 10.5423/RPD.2016.22.1.32
In this work, major outer capsid protein (P10) encoded by genome segment S10 of Rice black-streaked dwarf virus (RBSDV) was expressed in Escherichia coli. Genomic dsRNA was extracted from RBSDV-miryang isolate infected rice plants. Based on the sequence of S10 (RBSDV-miryang, GenBank JX994211), a pair of S10 specific primers were designed and used to amplify the fragment encoding the N-part of P10. We amplified the partial gene (S10 1-834 nt) of RBSDV P10 (1-278 aa) by RT-PCR. Amplified RBSDV S10 (1-834 nt) was cloned into the expression vector pET32a (+). Recombinant RBSDV S10 (1-834 nt) was expressed in E. coli BL21(DE3) and purified by nickel-nitrilotriacetic acid (Ni-NTA) affinity column. We successfully obtained P10 partial protein of RBSDV and the purified protein was used to immunize rabbits. The resulting polyclonal antiserum specifically recognized RBSDV from infected plant in both Western blotting and enzyme-linked immunosorbent assay. In this study, we provide purified RBSDV P10 (1-278 aa), which would be good material for the serological study of RBSDV-miryang isolates.
Incidence of Wildfire Disease on Soybean of Korea during 2014-2015
Kang, In Jeong ; Kim, Seung-Han ; Shim, Hyeong Kwon ; Seo, Min Jeong ; Shin, Dong Bum ; Roh, Jae Hwan ; Heu, Sunggi ;
Research in Plant Disease, volume 22, issue 1, 2016, Pages 38~43
DOI : 10.5423/RPD.2016.22.1.38
Wildfire caused by Pseudomonas amygdali pv. tabaci has been seen a steady increase in the incidence and prevalence on soybean in Korea. But up to know there is no official report on the prevalence of the disease in Korea. The nationwide survey of the occurrence of soybean wildfire showed that 19 out of 30 regions in 2014 and 9 out of 28 regions in 2015 had been infected with P. amygdali pv. tabaci. The severity of wildfire varied according to the cultivar, cultivation regions and climates. However, cultivar seoeitae was susceptible in both 2014 and 2015. The disease severity of 2014 was twice that of 2015. Since the temperature of 2014 and 2015 were very similar but the precipitation of 2014 was more than twice that of 2015, the precipitation of 2014 had a great impact on the wildfire disease severity.
Twig Blight on Chinese Magnolia Vain Caused by Botryosphaeria dothidea in Korea
Park, Sangkyu ; Kim, Seung-Han ; Lee, Seung-Yeol ; Back, Chang-Gi ; Kang, In-Kyu ; Jung, Hee-Young ;
Research in Plant Disease, volume 22, issue 1, 2016, Pages 44~49
DOI : 10.5423/RPD.2016.22.1.44
The twig blight symptoms were observed in Chinese magnolia vine (Schisandra chinensis) at Mungyeong city, Gyeongbuk province, Korea in June 2015. The typical symptoms of infected plant were shriveled and wilted in leaves which led to blight resulted in death. Based on the morphological characteristics, the isolate was suspected as Botryosphaeria sp. Inoculation of isolated pathogen was performed to identify its pathogenicity according to Koch`s postulates. Re-isolated fungi from the inoculated stem was showed same morphological characteristics with original pathogen. Phylogenetic analysis was performed using combined sequence of rDNA internal transcribed spacer region, EF1-
-tubulin gene. The isolated pathogen was identified to the B. dothidea by phylogenetic analysis. This is the first report of twig blight on S. chinensis caused by B. dothidea in Korea.
Alternaria Leaf Spot Caused by Alternaria mali on Black Chokeberry in Korea
Hahm, Soo-Sang ; Kwon, Mi-Kyung ; Kim, Byung-Ryun ; Han, Kwang-Seop ; Nam, Yun-Gyu ;
Research in Plant Disease, volume 22, issue 1, 2016, Pages 50~54
DOI : 10.5423/RPD.2016.22.1.50
In early June 2014, leaf spot symptoms were observed on black chokeberry (Aronia melanocarpa) in Yesan-gun and Geumsan-gun, Chungcheongnam-do in Korea. The initial symptoms on leave surfaces were brown small-circular spots with a yellow halo lesion, and gradually the small spots were fused, all of infected leaves dropped eventually. A fungus were isolated from the initial lesion, and cultured on potato dextrose agar. Colony color on upper surface of plate varied from olive gray to charcoal gray. Size of conidia mostly extend to
in nature and
in culture, with 3-8 transverse septa and usually no longitudinal septum or only 1 longitudinal septum in 1-3 of the transverse compartments, and also have a short or long beak. Pathogenicity was investigated using wounded or unwounded black chokeberry and apple leaves. After 7 days of inoculation, leaf spots were similar to the symptoms naturally occurred in the field. On the basis of mycological characteristics, pathogenicity, and ITS rDNA sequence analysis, this fungus was identified as Alternaria mali. This is the first report of Alternaria leaf spot on black chokeberry caused by A. mali in Korea.
Characterization of Cucumber mosaic virus Isolated from Trifolium repens in Korea
Park, Tae Seon ; Choi, Gug Seoun ; Hong, Jin Sung ;
Research in Plant Disease, volume 22, issue 1, 2016, Pages 55~58
DOI : 10.5423/RPD.2016.22.1.55
A Cucumber mosaic virus (named CMV-Tr1) isolated from the white clover (Trifolium repens) showing mosaic and malformation that found in a pepper field. Cucumber mosaic virus was identified through confirmation with PT-PCR, PCR-restriction fragment length polymorphism, and sequence analysis of coat protein (CP) gene. CMV-Tr1 mosaic symptom on the upper leaves of five tobacco species including Nicotiana benthamiana, Cucumis sativus, Physalis angulata, and Solanum lycopersicon. In Chenopodium quinoa and Vigna unguiculata the isolate showed local lesions in inoculated leaves. CMV-Tr1 compared with CMV-As in the sequence identity of CP gene. CMV-Tr1 showed 98.9% and 99.5% homologies at nucleotide and amino acid levels, respectively. Phylogenetic analysis of the CP gene indicated that CMV-Tr1 belongs to the CMV subgroup IB base on the CP. To our knowledge, this is the first report of CMV in T. repens in Korea.
Identification of Daphne Mottle Virus Isolated from Daphne odora, a New Member of the Genus Potyvirus
Park, Chung Youl ; Park, Jungan ; Lee, Boo-Ja ; Bak, Sangmin ; Lee, Hong-Kyu ; Kim, Jeong-Sun ; Yoon, Youngnam ; Suh, Sang Jae ; Lee, Su-Heon ;
Research in Plant Disease, volume 22, issue 1, 2016, Pages 59~63
DOI : 10.5423/RPD.2016.22.1.59
A new poty-like virus was isolated from plants of winter daphne (Daphne odora) that showed virus-like symptoms on leaves, from four regions of Korea during 2014. Filamentous-shaped particles were observed by transmission electron microscopy of preparations extracted from symptomatic leaves and examined by the direct negative stain method. RT-PCR assay showed that three samples were positive for both Cucumber mosaic virus and potyvirus, and only one sample was positive for potyvirus only. A BLAST comparison to partial sequences from helper-component proteinase, cylindrical inclusion and coat protein genes detected the highest nucleotide identity of 76%, 72%, and 72% with Daphne mosaic virus, respectively, levels below the potyvirus species discrimination threshold. The new potyvirus was isolated using indicator plants (Chenopodium amaranticolor), in which local lesions were produced. In this study, we identified a novel potyvirus from winter daphne, which we have named Daphne mottle virus (DapMoV).