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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Research in Plant Disease
Journal Basic Information
Journal DOI :
Korean Society of Plant Pathology
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Volume & Issues
Volume 3, Issue 4 - Dec 1987
Volume 3, Issue 3 - Sep 1987
Volume 3, Issue 2 - May 1987
Volume 3, Issue 1 - Jan 1987
Selecting the target year
Differentiation of Colletotrichum spp. Causing Anthracnose on Capsicum annum L. by Electrophoretic Method
Park Won Mok ; Park Sang Ho ; Lee Yong Se ; Ko Young Hee ; Cho Eui Kyoo ;
Research in Plant Disease, volume 3, issue 2, 1987, Pages 85~85
The present researches were carried out to differentiate the species of Colletotrichum by elecrophoretic method. C. gloeosporioides, C. dematium and Gloeosporium fructigenum could be differentiated by the isozyme patterns of esterase, leucine aminopeptidase, acid phosphatase and glutamic oxaloacetic transaminase. Especially. G-strain and R-strain of C. gloeosporioides were differentiated by the enzyme patterns. The Gstrain damaged all stage fruits (green and red fruits) of Capsicum annum. The R-strain could not infect the unripe (green) fruits, but it could damage only ripe (red) fruit of Capsicum annum.
Carbohydrate, Amino Acid and Phenolic Contents of Rice Leaves in Relation to Adult-Plant Resistance to Leaf Blast
Hwang Byung Kook ; Kim Ki Deok ;
Research in Plant Disease, volume 3, issue 2, 1987, Pages 93~93
Six rice cultivars showing various types of resistance or susceptibility to Pyricularia oryzae in the field were used to study the relationship between susceptibility and contents of carbohydrates, amino acids and phenolics at different plant maturities and leaf ages under controlled environmental conditions. No consistent differences between the susceptible cultivars and adult-plant-resistant cultivars were found in terms of the contents of individual carbohydrates, total amino acids and phenolics in rice leaves throughout the plant development. Only the adult-plant-resistant cultivar Dobong had lower contents of inositol throughout the plant development than the other cultivars. The amounts of sucrose, inositol, glucose, and fructose increased in all tested cultivars at eight leaf stage than those at five leaf stage, but slightly decreased at twelve leaf stage. In contrast, total amino acids and phenolics gradually decreased during plant development. With increasing age of rice leaves, the amounts of total soluble amino acids and phenolics gradually increased in healthy fifth leaf tissues, although there were no significant differences between the cultivars. In particular, a high level of phenolics existed in old fifth leaves of the cultivar Dobong. These results suggest that increased resistance to blast of matured rice plants and old leaves may be the two different phenomina derived from the physiological changes occurring during plant development and leaf senescence, probably functioning differently in P. oryzae development.
Effect of Soil Moisture on the Pre-Penetration Activity of Pyricularia oryzae Cav. on Rice Leaf Epidermis
Kim Choong Hoe ;
Research in Plant Disease, volume 3, issue 2, 1987, Pages 100~100
Pre-pentration activity of Pyricularia oryzae Cav. from the stage of conidia germination to appressorium formation was examined on rice leaf epidermis under light and scanning electron microscopes to determine the causes· for differences in blast susceptibility between plants grown under three different soil moisture conditions in the greenhouse. No significant differences were found in the external shape of leaf epidermal cells including bulliform cells between plants grown under different soil moisture conditions. Growth and orientation of germ tube and morphology and size of appressorium of P. oryzae did not vary with soil moisture treatment. Site of appressorium formation was consistent over soil moisture treatment with the highest frequency of bulliform cell
, followed by short cell
, and long and guard cells
. No appressorium was formed on trichome. This result suggests that the observed differences in blast susceptibility between plants grown under different soil moisture conditions were not due to the differences in the pre-pentration activity of P. oryzae on those plants.
Detection of Rice Black-Streaked Dwarf Virus In Rice, Maize and Insect Vectors by Enzymelinked Immunosorbent Assay
Woo Yong Bum ; Lee Key Woon ;
Research in Plant Disease, volume 3, issue 2, 1987, Pages 108~108
Rice black-streaked dwarf virus(RBSDV) was purified from infected maize leaves. Antiserum against RBSDV was prepared for virus detection by enzyme-linked immunosorbent assay(ELISA). In detection of RBSDV by ELISA, effective dilution range of antiserum extracted in RBSDV-containing host plants and insect vectors was from 320 to 2,560 times in rice plant, 320 to 5,120 in maize plant, and 160 to 2,560 times in insect vector, Laodelphax striatellus F, respectively. The percentage of viruliferous vector in overwintered nymphs of Laodelphax striatellus determined by ELISA were 3.0 in Milyang, 2.3 in Chilgok, and 3.7 in Sunsan area. Dead insect vector which could not be tested for vims infection by conventional rice seedling inoculation test could be tested by ELISA. One hundred plants of rice and maize were randomly sampled in the field and tested whether or not they were infected with RBSDV. In rice plants, 4 out of 98 plants turned out to be infected with RBSDV by ELISA. In maize plant, 3 out of 92 plants which were excepted 8 plants to be appeared symptom already were infected. As a result, ELISA could be detected even in case of symptomless plants at early stage of viral infection.
Banana Blast Caused by Pyricularia angulata Hashioka
Kim Wan Gyu ; Kim Chang Kyu ; Lee Eun Jong ;
Research in Plant Disease, volume 3, issue 2, 1987, Pages 114~114
Banana blast was frequently encountered in greenhouses of Jeju province during a local survey of plan diseases from 1985 to 1986. The disease incidence on banana was very severe up to
in a greenhouse. The causal fungus was identified as Pyricularia angulata Hashioka based on mycological characteristics and Path genicity of the fungus. The symptom of blast produced on leaves of banana was circular to oval, dark brow spots with yellow margins which measured 1-10mm in diameter, and the symptom on fruits was circular to oval reddish brown or dark brown, depressed spots which measured up to 20mm in diameter. The symptoms were scattered not only on leaves and fruits of banana but. also on petioles, leaf sheaths, bunch stalks and crowns The pathogen was characterized that conidia were hyaline, 2 septated, ovate to pyriform with a small hilum solitary at ends of den tides of conidiophores, and measured
) and conidiophores were mostly 2 septated, occasionally 2 or 3 branched, denticulate at the tips, and measured
). The optimum temperature range for mycelial growth of the fungus on PDA was
. The result of pathogenicity tests revealed that P. angulata had pathogenicity only on banana. On the other hand Pyricularia grisea (Cke.) Sacc. isolated from Digitaria sanguinalis(L.) Scopo. was not pathogenic to banana. It was revealed that the leaves were the most susceptible to P. angulata among several parts of banana in inoculation tests. Thirty-day-old fruits of banana were more susceptible to the fungus than 60-day-old fruits, and bunch stalks were more susceptible than the fruits.
Epidemiological Studies of Rice Blast Disease Caused by Pyricularia oryzae Cavara I. Measurement of the Amount of Spores Released from a Single Lesion
Kim Chang Kyu ; Yoshino Reiichi ;
Research in Plant Disease, volume 3, issue 2, 1987, Pages 120~120
Four types of spore trap (Kim''s original, improved Kim''s original, Yoshino''s original and mixed type of Kim''s and Yoshino''s original) were evaluated for their efficacy to "estimate the amount of spores released from leaf blast lesions under the natural conditions. It was found that all four types had one or two defects in allowance for adequate sporulation/release, spore catch or spore counting. Thus, an improved type of spore trap was devised considering that it could cover the defects mentioned above. As a result, newly developed spore trap was quite satisfactory in above mentioned aspects and it could be used for pursuit of spore release phase under the natural conditions.
Purification and Cell Wall Regeneration of Protoplasts from Pyricularia oryzae Cav.
Han S. S. ; Lee Y. H. ; Yoo J. D. ; Lee E. J. ;
Research in Plant Disease, volume 3, issue 2, 1987, Pages 124~124
The optimum conditions for protoplast formation and regeneration from Pyricularia oryzae Cav. were selected as follows. As a basic solution, 0.02M potassium phosphate buffer solution plus 0.6M KCl adjusted to pH 5.2 with 1N HCl was used. A mixture of enzyme combinations with 20mg Cellulase R-l0/ml, 5mg Macerozyme R-l0/ml and l0mg Driselase/ml used as a lytic enzyme showed better lytie effect than any single enzyme treatment for protoplast formation. Two-day-old mycelia of P. oryzae grown in the mixture of three lytie enzyme solution at
for 3 hr showed best condition for protoplasts formation. For regeneration from the protoplasts of P. oryzae, potato dextrose agar containing 0.02M potassium phosphate plus 0.6M KCl used as a stabilizer was best for regeneration medium
Disease Dispersal Gradients of Rice Blast from a Point Source
Kim Choong Hoe ;
Research in Plant Disease, volume 3, issue 2, 1987, Pages 131~131
Rates of lesion development over time and disease gradients over distance for blast disease on the two rice varieties, Brazos and M-20 1 were significantly affected by two different cultural conditions, upland and flooded conditions. Flooding rice field plots lowered the rates of lesion increase and flattened the disease gradients for both varieties. Despite absence of statistically significant differences in the rate of lesion increase between four sampled distances from infection focus, rate of lesion development tended to be slightly greater as distance from the infection focus increases. Rate of lesion increase was greater with more susceptible variety M-201 than with Brazos. Disease gradient was steeper for M-201 than for Brazos. As blast disease progressed, disease gradients became flattened regardless of variety due to the infections originated from secondary foci. Between two empirical disease gradient models examined, Kiyosawa & Shiyomi model was fitted better over Gregory model. Rates of blast isopath movement under upland conditions were calculated as approximately 0.2m/day and 0.4 m/day for Brazos and M-201, respectively. The results in this study suggest that differences in varietal resistance to blast could be detected by measuring disease gradient as efficiently as by measuring infection rate.
Pathogenicity of Fusarium moniliforme Sheld. Isolated from Banana(Musa sapientum L.)
Kim Wan Gyu ; Kim Chang Kyu ;
Research in Plant Disease, volume 3, issue 2, 1987, Pages 137~137