Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Research in Plant Disease
Journal Basic Information
Journal DOI :
Korean Society of Plant Pathology
Editor in Chief :
Volume & Issues
Volume 3, Issue 4 - Dec 1987
Volume 3, Issue 3 - Sep 1987
Volume 3, Issue 2 - May 1987
Volume 3, Issue 1 - Jan 1987
Selecting the target year
Distribution of Root-knot Nematodes, Meloidogyne spp. and Their Races in Economic Crops in Korea
Cho H. J. ; Kim C. H. ; Park J. S. ; Jeoung M. G. ;
Research in Plant Disease, volume 3, issue 3, 1987, Pages 159~159
In order to investigate the distribution and density of root-knot nematodes in economic crops, samples were collected from 3,226 fields of 18 economic crops. Nematodes were extracted using a modified Baermann''s funnel technique. Identification of races was based on the differential host-test for Meloidogyne spp. by Tayler and Sasser. M. hapla was dominant in fields in the middle parts of Korean peninsula; M. incognita, in the southern parts, and distribution of M. javanica was limited to Jeju island and southern seaside areas. Two races of M. arenaria identified in this study were races 1 and 2. The three races identified in M. incognita were races, 1, 2 and 3. Of these races, race 1 of M. arenaria and race 3 of M. icognita were identified for the first time in Korea.
Effects of Root-knot Nematodes, Meloidogyne hapla, M. incognita, M. arenaria and M. javanica on Growth and Yield of Tomato
Cho H. J. ; Kim C. H. ; Park J. S. ; Jeoung M. G. ;
Research in Plant Disease, volume 3, issue 3, 1987, Pages 164~164
The effects of infection severity of susceptible tomato varieties, Rutgers and Boksu 2, by root-knot nematode, meloidogyne hapla, M. arenaria or M. javanica on plant growths and tomato yields were investigated. The inoculum levels of each nematode species were 0, 1,000 and 10,000 nematodes per 42cm diam. pot. Tomato yield was reduced by the nematode infection. Severity of infection was on the decreasing order of M. javanica, M. incognita, M. arenaria, M. hapla, ranging from
by M. javanica to only
by M. hapla. Yield reduction by infection of M. hapla was more prominent in Boksu 2 than in Rutgers. However the results were vice versa for the other nematode species, The top fresh-weight of Rutgers inoculated with 10,000 nematodes was greater than root weight, regardless of the nematode species, whereas plant height and top fresh weight decreased with increasing root weight when inoculated with inoculum density of 1,000/pot.
Esterase Isozyme of Mycelium of Pyricularia oryzae under Various Cultural Conditions
Park Sang Ho ; Park Won Mok ; Kim Seong Hoe ; Lee Eun Jong ;
Research in Plant Disease, volume 3, issue 3, 1987, Pages 168~168
The constancy of the patterns of esterase isozymes in the mycelium of Pyricularia oryzae grown under various cultural conditions was observed by
polycrylamide gradient slab gel. The position of the isozyme was not affected by cultural age for 24 days. However the intensity of the band was affected. The band patterns were not affected by the carbon sources (glucose, maltose, fructose, sucrose or starch), single spore isolates, successive transfer culture and regenerated isolates from protoplast cultures.
Regional and Annual Fluctuation of Races of Pyricularia oryzae During 1978-1985 in Korea
Ryu J. D. ; Yeh W. H. ; Han S. S. ; Lee Y. H. ; Lee E. J. ;
Research in Plant Disease, volume 3, issue 3, 1987, Pages 174~174
Four thousand eight hundred and eighty five isolates of Pyricularia oryzae were obtained from the diseased rice specimens collected from various areas of Korea for race identification during 1978 - 1985. A total of 15 races inculding 6 races pathogenic to Tongil lines, 2 T-races, 5 C-races and 2 N-races was identified using a old Japanese differential set during 1978-1980. Since 1981, number of races identified by a Korean differential set was 18 races which were composed of 11 KI-races pathogenic to either Tongil lines or Japonica cultivars and 8 KJ-races pathogenic to only Japonica cultivars. The prevalent race was
during 1978-1979 and race KJ-301 since 1980, respectively. Races KI-315a and KI-315b pathogenic to most of Tongil lines were identified in 1983. being widely distributed to date. Races KJ-105 and KJ-201 pathogenic to the cultivars possessing resistance genes Pi-k and Pi-i were prevalent in Gangweon province, whereas race KI-315b was prevalent in Chungbug and Jeonnam districts.
Ice-Nucleation Activity of Pseudomonas syringae Isolated in Korea
Kim Yong Hwan ; Kim Young Cheol ; Cho Baik Ho ; Kim Ki Chung ;
Research in Plant Disease, volume 3, issue 3, 1987, Pages 180~180
Cell suspensions of two isolates of Pseudomonas syringae. PS8401 from sweet persimon and PS8402 from tea plant, were active in ice nucleation at -2.5 and
, respectively. Ice nucleation at those temperature was, using micropipette method, detected in suspensions (
olony forming unit/ml of distilled water) of cells that had been grown on nutrient agar supplemented with
glycerol. Using the same method, on the other hand, the freezing temperature of distilled water only was approx.
, and those of various plant saps including corn were lower than
. Corn seedlings sprayed with cell suspensions
of nutrient broth) of PS8401 began to be damaged at
and were almost completely damaged at
, whereas seedlings sprayed with nutrient broth only were not injured until the temperature down to
. Amounts of frost damage measured 48 hr after application of PS8401 suspensions increased as applied bacterial cell densities were increased. Ice-nucleation activity of the cell suspensions in vitro increased with increasing the number of cells in suspension. The activity also affected by growth-medium composition or growth-temperature. Ice nucleation thus occured at -4.0, -4.4 and
of PS8401 that had been grown at
nutrient agar with
glycerol, nutrient agar with
glucose and nutrient agar only, respectively, and occured at -4.0 and
of PS8401 hat had been grown on nutrient agar with
Isolation, Identification and Antagonisms of Rhizospheric Antagonists to Cucumber Wilt Pathogen, Fusarium oxysporum f. sp. cucumerinum Owen
Jee Hyeong Jin ; Kim Hee Kyu ;
Research in Plant Disease, volume 3, issue 3, 1987, Pages 187~187
Bacteria and fungi antagonistic to Fusarium oxysporum f. sp. cucumerinum Owen were effectively isolated with each of modified Triple Layer Agar (TLA) technique from rhizosphere soil where cucumber had been grown healthily in plastic film house. Three predominant bacterial isolates selected were identified as Pseudomonas fluorescens, and P. putida, Serratia sp. and three fungal isolates were Gliocladium sp. Trichoderma harzianum, and T. viride. Antagonistic bacteria inhibited
of germination and
of germ tube elongation of microconidia of F. oxysporum f. sp. cucumerinum on Water Agar (WA). P. fluorescens was the strongest inhibitor. Several my co parasitisms were observed on dual culture of WA between antagonistic fungi and F. oxysporum f. sp. cucumerinum such as coiling, penetration, overgrowing, and lysis. Mycelial lysis of the pathogen was the most severe at pH 4.6, followed by 3.6, 5.6 and 6.6 of the medium in decreasing order. At pH 6.6, mycelia of the pathogen were not conspicuously damaged, however, the antagonistic fungi formed abundant chlamydospores especially Gliocladium sp. T. harzianum revealed the most excellent antagonism in vitro.
Measurement of Dilution End-Points and Phytotoxicity of Toxic Metabolites Produced by Helminthosporium sativum in Barley, Wheat and Lettuce Roots
Lee Sang. S. ;
Research in Plant Disease, volume 3, issue 3, 1987, Pages 198~198
Toxic metabolites (''Toxins''), produced by Helminthosporium sativum causing leaf blotch in barley and root rot in barley and wheat were partially purified through C-18 column. The partially purified toxins appeared heat unstable and lipophilic. The responses of toxins to wheat and barley root corresponded with those to lettuce growth with the different concentrations. The determination of the concentration of toxins produced was developed using the dilution end-points. The equation [Y = a log X + b) was obtained from the semi-loggraphy with the linear analysis. The values ''a'' and ''b'' were discussed with the responses of several plants on the toxin produced by H. sativum.
Production of Antibacterial Substance, and Changes in Peroxidase nd Polyphenoloxidase Activities in Tobacco Plants Inoculated with Avirulent Isolate of Pseudomonas solanacearum
Yi Young Keun ; Min Tae Gi ; Park Won Mok ;
Research in Plant Disease, volume 3, issue 3, 1987, Pages 203~203
The substances obtained from the leaf, stem and root of tobacco plants inoculated with avirulent and virulent isolates of Pseudomonas solanacearum were at
on TLC plate, respectively. Both substances showed antibacterial activities not only on P. solanacearum but also on Erwinia carotovora subsp. carotovora and Escherichia coli in vitro. However, the antibacterial substances were not detectable from the filtrate of the autoclaves tobacco sap medium, in which the avirulent or virulent bacterium was cultured for 3 days. Peroxidase and poly phenoloxidase activities and their isozyme patterns did not differ significantly between plants treated with the virulent and avirulent isolates, or between the susceptible cultivar BY 4 and the resistant cultivar NC 82. However, activities of the two enzymes were increased in leaves of the susceptible cultivar BY 4 treated with either the virulent or the avirulent isolate.
A Model to Forecast Rice Blast Disease Based on Weather Indexing
Kim Choong-Hoe ; MacKenzie D. R. ; Rush M. C. ;
Research in Plant Disease, volume 3, issue 3, 1987, Pages 210~210
A computer program written to predict blast occurrence based on micro climatic events was developed and tested as an on-site microcomputer in field plots in 1984 and 1985. A microcomputer unit operating on alkaline batteries; continuously monitored air temperature, leaf wetness, and relative humidity; interpreted the microclimate information in relation to rice blast development and displayed daily values (0-8) of blast units of severity (BUS). Cumulative daily BUS values (CBUS) were highly correlated with blast development on the two susceptible cultivars, M-201 and Brazos grown in field plots. When CBUS values were used to predict the logit of disease proportions, the average coefficients of determination
between these two factors were 71 to
, depending on cultivar and year. This was a significant improvement when compared to 61 to
when days were used as a predictor of logit disease severity. The ability of CBUS to predict logit disease severity was slightly less with Brazos than M-201. This is significant inasmuch as Brazos showed field resistance at mid-season. The results in this study indicate that the model has the potential for future use and that the model could be improved by incorporating other variables associated with host plants and pathogen races in addition to the key environmental variables.
Pathogenicity of Rhizoctonia Isolates from Southern Horticultural Area in Korea
Roh Myung Ju ; Kim Hee Kyu ;
Research in Plant Disease, volume 3, issue 3, 1987, Pages 217~217
Pathogenicity of nine Rhizoctonia solani isolates of different anastomosis groups (AG) on seed and hypocotyls of red pepper, cucumber, Chinese cabbage and radish varied considerably from nonvirulent to highly virulent. Rhizoctonia solani AG 1 was highly virulent on the above four plant species. AG 2 type 1 was highly virulent on radish and Chinese cabbage, moderately virulent on red pepper, and AG 2 type 2 was avirulent or weakly virulent except red pepper. R. solani AG 5 was moderately virulent on hosts tested. In general, virulence of the R. solani isolates to a given host varied among anastomosis groups, but not within anastomosis groups. Anastomosis groups lacked host specificity. The pathogenicity was stronger in steam-sterilized soil than in non-sterilized field soil, if the inoculated plants were closely related with orginal host from which the pathogen was isolated. On the other hand, pathogen was more virulent in non-sterilized field soil than in steam-sterilized soil, if the inoculated ones were not closely related. Generally, contrary to other soil-brone plant pathogenic fungi, Rhizoctonia isolates tended to be more virulent in non-sterilized field soil than in the same soil which had been steamed. A potential danger of building up propagules of R. solani in southern horticultural area are discussed in terms of cropping system.
Studies on Viruses Isolated from Lilium spp. In Korea I. Broad Bean Wilt Virus and Cucumber Mosaic Virus
Chang Moo Ung ; Chung Jae Dong ;
Research in Plant Disease, volume 3, issue 3, 1987, Pages 223~223
Samples showing yellow mosaic symptom of Lilium spp. with necrotic fleck, stunting, malformation, and colour breaking were collected from lily-growing areas in the southern part of Korea. Two viruses were distinguished under a electron microscope and their host range, serological reaction, stability in sap, type of aphid transmission, and relations with cells and tissues were examined. Broad bean wilt virus (BBWV) was transmitted by sap-inoculation to 23 plant species in 8 families and by the aphid, Myzus persicae. This virus was inactivated after 10 min at 70C, at dilution of
, and after 6 days at about 20C. Electron microscopic examination of purified preparation showed that the virus is spherical particle of 28nm in diameter. The virus reacted positively with BBWV-antiserum in agar gel diffusion test. In ultrathin sections of BBWV infected tissues, large aggregates or crystalline array of virus particles and vesicular body were found in the cytoplasm, vacuole, and nucleus of mesophyll cells. Cucumber mosaic virus (CMV) was transmitted by sap-inoculation. Electron microscopic examination of its purified preparation showed spherical particles of 30nm in diameter. The virus reacted positively with CMV-Y strain-antiserum in agar gel diffusion test. In ultrathin sections of CMV infected tissues, crystalline array of virus particles were found in the vacuole and a large number 0f virus particles were found in the cytoplasm and the plasmodesmata of mesophyll cells. When each of these viruses was retransmitted to Lilium tigrinum. L. concolor, and L. auratum, BBWV induced slight symtoms and colour breaking, but CMV induced yellowing mosaic or necrotic fleck.
Bacterial Soft Rot of Elephant Foot Caused by Erwinia carotovora subsp. carotovora
Choi Jae Eul ; Park Jong Seong ; In Moo Seung ; Ahn Byeong Chang ;
Research in Plant Disease, volume 3, issue 3, 1987, Pages 236~236
A bacterial disease of elephant foot, Hydrosome rivieri Engl., was newly found in Taejon, Korea in August 1986. The affected plants showed symptoms of soft rot on leaf blades and petioles. Bacterial isolates from affected plants found to be pathogenic to elephant foots by antificial inoculation, producing similar symptoms with those produced naturally. The baterium was also pathogenic to carrot, Chinese cabbage, radish, potato and onion, and developed symptom of soft root on them. On the basis of bacteriological characteristics and pathogenicity, tested bacterium was identified as Erwinia carotovora subsp. carotovora and this disease was proposed to name ''Bacterial soft rot of elephant foot''.