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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Research in Plant Disease
Journal Basic Information
Journal DOI :
Korean Society of Plant Pathology
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Volume & Issues
Volume 7, Issue 3 - Dec 2001
Volume 7, Issue 2 - Aug 2001
Volume 7, Issue 1 - Apr 2001
Selecting the target year
Phytotoxins of Pseudomonas syringae and PCR Primers for Detection of Phytotoxin-Producing Strains
Research in Plant Disease, volume 7, issue 3, 2001, Pages 123~133
Many pathovars of the species Pseudomonas syringae are known to produce different phytotoxins as secondary metabolites. Although phytotoxins generally enhance the virulence of P. syringae, they are not required for pathogenesis. Among the phytotoxins produced by P. syringae, lipodepsipeptides, coronatine, phaseolotoxin, and tabtoxin are the most well-known toxins which have been intensively studied for their structure, mode of action, biosynthesis, and regulation. A polymerase chain reaction (PCR) technique that amplifies a segment of the phytotoxin gene cluster using a primer set has been developed in recent years. This method offers the advantages of speed and sensitivity compared to the approaches based on physiological and biochemical methods. PCR detection of genes involved in the production of toxins could be exploited for early diagnosis of plant diseases caused by P. syringae pathovars.
Effects of Tomato-Juice and Potassium Phosphate on the Infection of Botryis cinerea LVF12 on the Tomato Leaves
Research in Plant Disease, volume 7, issue 3, 2001, Pages 134~139
Effects of tomato-juice and KH
as exogenous nutrients on the infection of Botrytis cinerea LVF12 and pathogenicity to tomato were investigated. B. cinerea LVF12, which was previously reported as a casual agent of the gray mold rot of perilla, was used for pathogenesis on tomato leaves. No infection was induced, and no lesion developed on tomato leaves by the conidial suspension of LVF12 when the inoculum was prepared in sterilized water. However, when the conidial suspensions of LVF12 added with various concentrations and conditions of tomato-juice were inoculated on whole tomato plants, the disease was induced readily, Among them, 20% tomato juice with 0.1M KH
appeared to be the most suitable nutrient to promote high disease incidence on tomato. For the pathogenicity test according to the growing stage of tomato, the mature leaves were more susceptible than seedlings. Symptoms on the infected plants were initial small gray spots at the inoculated area. Later the whole leaves, petioles and stems became gray and eventually fell off, Under high humidity conditions, the diseased leaves and stems were covered with gray hyphae and conidia. All symptoms of infected plants were identical to those in the field conditions.
Etiological Characteristics and Chemical Control of Ripe Rot in Grape Cultivar Campbell Early
Research in Plant Disease, volume 7, issue 3, 2001, Pages 140~144
Occurrence of ripe rot in Vitis vinifera cv, Campbell Early was examined at Daegu, Yaungchon, and Kimchon in Kyungbuk province, Korea from 1997 through 1999. Its occurrence was related to rainfall days in July and Auguest. In cv, Campbell Early, the disease started in late July, and increased rapidly from mid August. Considering the incubation time, the pathogen infection might have increased from late July, which was confirmed by wrapping grape clusters in field and in vitro tests. By scanning electron microscopy, it took 24 h or longer for infection to occur in moisturized conditions. Chlrorothalonyl WP (500
) sufficiently reduced ripe rot disease when the chemical was sprayed 3 or 4 times during the growing season.
The Bacterial Gall of Wistaria floribunda Caused by Pantoea agglomerans pv, milletiae
Research in Plant Disease, volume 7, issue 3, 2001, Pages 145~149
A pathogenic bacterium was isolated from galls on Wistaria floibunda at Jinryang of Kyungsan, Kyungbuk, Korea in May, 2000. This bacterium was cultured and tested for pathogenicity on the host. Also its morphological and physiological characteristics were examined. Inoculation with the bacterium isolated from the gall caused the same symptoms as those under natural conditions. On the basis of bacteriological characteristics and pathogenecity on the host plant of the organism, the causal bacterium was identified as Pantoea agglomerans pv. milletiae. This is the first report of this bacterium to occur on W. floribunda plant in Korea. Therefore we proposed to name the disease as "bacterial gall of Wistaria floribunda" by P. agglomerans pv. milletiae.milletiae.
Influence of Weather Condition at Heading Period on the Development of Rice Bacterial Grain Rot Caused by Burkholderia glumae
Research in Plant Disease, volume 7, issue 3, 2001, Pages 150~154
The relationships between the weather conditions and the occurrence of rice bacterial grain rot caused by Burkholderia glumae during the rice heading period, were examined by analying the data accumulated from 1992 through 2000 and by conducting greenhouse and field experiment to develop a model far forecasting the disease. The disease severely occurred in 1994, 1995, 1998, and 2000, when it was high in temperature and rainfall during the heading period of middle-late rice varieties. While it occurred weakly in 1993 was high in rainfall and low temperature and it was reversely in 1997. When treated under wetting condition (above the 24-hour)after inoculation at heading period, the infection rate increased as the inculum concentration increased, it was 86.1% at 10
cfu/ml. When under drying condition, the disease of 12.5% occurred only at
cfu/ml. On the other hand, 1,000 grain weight and the percentage of ripened grains remarkably decreased as the infection rate increased.
Cross-Protection Effectiveness of Cucumber mosaic virus (CMV) Isolates Associated with Satellite RNA for Prevention of CMV Disease in Pepper Plants
Research in Plant Disease, volume 7, issue 3, 2001, Pages 155~163
Two attenuated Cucumber mosaic virus (CMV) isolates, Paf-CMV and Rs2-CMV that had been selected from CMV isolates associated with satellite RNA (satRNA) were tested for cross-protection effect in pepper plants. The viruses selected as attenuated strains appeared to be identical serologically and physically to the challenge virus (Mf-CMV), but they were lower in the dilution end-point of infectivity of crude sap than Mf-CMV When symptoms were observed in several indicator plants after inoculation, Paf-CMV and Rs2-CMV were symptomless or showed mild mosaic symptoms while another satRNA isolate Ap-CMV developed severe mosaic symptoms on the leaves as Mf-CMV. The nucleotide sequences of the satRNAs were determined by sequencing full-length cDNA clones. Paf-, Rs2- and Ap-satRNAs were 386, 335, and 347 nucleotides long, respectively, The sequences were then compared with the other known Y-satRNA, revealing that nucleotide sequences of the satRNAs consisted of 5'- and 3'-terminal conserved regions. However variations occurred on the middle regions of the sequences, especially those related to symptom interference, showing significant differences between Paf-satRNA and other isolates. Infectious transcripts of Paf-satRNA and Rs2-satRNA induced mild mosaic symptoms in pepper plants when supported by genomic RNAs of Mf-CMV. Under greenhouse conditions, Paf-CMV and Rs2-CMV were tested for cross-protection effect in pepper and tobacco (Nicotiana tabacum cv, Xanthi nc) plants against Mf-CMV. No symptoms were developed on the plants vaccinated with Paf-CMV until 3 weeks after inoculation with the virulent strain; however another attenuated isolate, Rs2-CMV, showed less effectiveness in cross-protection. Depending on the concentration of the challenged virus, symptoms sometimes appeared later in the upper leaves. However, in plants challenged with low concentrations (below 0.2 mg/ml) of the challenge inoculum, symptoms caused by the virulent strain did not develop on the plants vaccinated with Paf-CMV. In the field experiments, the number of pepper plants with severe mosaic symptoms in the control plots was progressively increased after transplanting and reached approximately 50% after 50 days. On the other hand, the incidence of mosaic disease appeared very low on the plants that had received the protective inoculation with Paf-CMV.
Identification and Characterization of Tobamoviruses Isolated from Commercial Pepper Seeds
Research in Plant Disease, volume 7, issue 3, 2001, Pages 164~169
Two Tobamoviruses showing different local lesion types on Nicotiana glutinosa was isolated from commercial pepper seeds. These viruses were designated Tobamovirus-6 (T-6) and Tobamovirus-19 (T-19). The biological and serological assays revealed that T-6 and T-19 were closely related to Pepper mild mottle virus (PMMoV) and Tomato mosaic virus (ToMV), respectively, The isolates also had low similarity in the array of viral coat protein gene sequences, of which T-19 was most identical to known strains of ToMV, while T-6 was closely related to PMMoV
Detection of Tobamoviruses and Survey on Contamination Rate in Commercial Pepper Seeds Using Gelatin Particle Agglutination Test
Research in Plant Disease, volume 7, issue 3, 2001, Pages 170~174
Gelatin particle agglutination test (GPAT) was optimized for detection of Tobamovirus and contamination of the virus in commercial pepper seeds was evaluated. The optimum concentration of
-globulin G, specific to tobacco mosaic virus pepper strain, was 100 ug/ml. The sensitivity of GPAT for the detection of Tobamovirus in pepper seeds was as high as enzyme-linked immunosorbent and dot immunoblotting assays. Optimum dilution ranges of the seed extract for GPAT was 5-25 folds. Using the optimized GPAT with above conditions, the rate of Tobamovirus contamination in seeds was turned out to be average of 79.1%.